LOCUS       DACELF010000001       109195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.001, whole genome shotgun sequence.
ACCESSION   DACELF010000001 DACELF010000000
VERSION     DACELF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 109195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.001"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(220..2112)
                     /locus_tag="IW687_000001"
     CDS             complement(220..2112)
                     /locus_tag="IW687_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949303.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2109..3836)
                     /locus_tag="IW687_000002"
     CDS             complement(2109..3836)
                     /locus_tag="IW687_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949304.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3852..4502)
                     /locus_tag="IW687_000003"
     CDS             complement(3852..4502)
                     /locus_tag="IW687_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0949305.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4706..5002
                     /locus_tag="IW687_000004"
                     /pseudo
     CDS             4706..5002
                     /locus_tag="IW687_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5174..5794
                     /locus_tag="IW687_000005"
     CDS             5174..5794
                     /locus_tag="IW687_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0949306.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5886..7052)
                     /locus_tag="IW687_000006"
     CDS             complement(5886..7052)
                     /locus_tag="IW687_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0949307.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7064..7417)
                     /locus_tag="IW687_000007"
     CDS             complement(7064..7417)
                     /locus_tag="IW687_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0949308.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7545..8567
                     /locus_tag="IW687_000008"
     CDS             7545..8567
                     /locus_tag="IW687_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0949309.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9006..9440)
                     /locus_tag="IW687_000009"
     CDS             complement(9006..9440)
                     /locus_tag="IW687_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949310.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9550..11409)
                     /locus_tag="IW687_000010"
     CDS             complement(9550..11409)
                     /locus_tag="IW687_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0949311.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11527..12687)
                     /locus_tag="IW687_000011"
     CDS             complement(11527..12687)
                     /locus_tag="IW687_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0949312.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12994..14418)
                     /gene="araA"
                     /locus_tag="IW687_000012"
     CDS             complement(12994..14418)
                     /gene="araA"
                     /locus_tag="IW687_000012"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0949313.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14434..15132)
                     /locus_tag="IW687_000013"
     CDS             complement(14434..15132)
                     /locus_tag="IW687_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0949314.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15089..16750)
                     /locus_tag="IW687_000014"
     CDS             complement(15089..16750)
                     /locus_tag="IW687_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0949315.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16968..18368
                     /locus_tag="IW687_000015"
     CDS             16968..18368
                     /locus_tag="IW687_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0949316.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18620..19699)
                     /locus_tag="IW687_000016"
     CDS             complement(18620..19699)
                     /locus_tag="IW687_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0949317.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19785..20744)
                     /locus_tag="IW687_000017"
     CDS             complement(19785..20744)
                     /locus_tag="IW687_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0949318.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20756..22279)
                     /locus_tag="IW687_000018"
     CDS             complement(20756..22279)
                     /locus_tag="IW687_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0949319.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22289..22852)
                     /locus_tag="IW687_000019"
     CDS             complement(22289..22852)
                     /locus_tag="IW687_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0949320.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22858..23721)
                     /locus_tag="IW687_000020"
     CDS             complement(22858..23721)
                     /locus_tag="IW687_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0949321.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23735..24631)
                     /locus_tag="IW687_000021"
     CDS             complement(23735..24631)
                     /locus_tag="IW687_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0949322.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24806..26077)
                     /locus_tag="IW687_000022"
     CDS             complement(24806..26077)
                     /locus_tag="IW687_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0949323.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26067..27584)
                     /locus_tag="IW687_000023"
     CDS             complement(26067..27584)
                     /locus_tag="IW687_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0949324.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27782..28672
                     /locus_tag="IW687_000024"
     CDS             27782..28672
                     /locus_tag="IW687_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0949325.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28745..29569)
                     /gene="nadE"
                     /locus_tag="IW687_000025"
     CDS             complement(28745..29569)
                     /gene="nadE"
                     /locus_tag="IW687_000025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0949326.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29581..31053)
                     /locus_tag="IW687_000026"
     CDS             complement(29581..31053)
                     /locus_tag="IW687_000026"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0949327.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31690..32052)
                     /locus_tag="IW687_000027"
     CDS             complement(31690..32052)
                     /locus_tag="IW687_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0949328.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32275..33027
                     /locus_tag="IW687_000028"
     CDS             32275..33027
                     /locus_tag="IW687_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0949329.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33167..34090)
                     /locus_tag="IW687_000029"
     CDS             complement(33167..34090)
                     /locus_tag="IW687_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0949330.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34105..34377)
                     /locus_tag="IW687_000030"
     CDS             complement(34105..34377)
                     /locus_tag="IW687_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949331.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34847..36472)
                     /gene="groL"
                     /locus_tag="IW687_000031"
     CDS             complement(34847..36472)
                     /gene="groL"
                     /locus_tag="IW687_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0949332.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36523..36807)
                     /locus_tag="IW687_000032"
     CDS             complement(36523..36807)
                     /locus_tag="IW687_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0949333.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37032..37694
                     /locus_tag="IW687_000033"
     CDS             37032..37694
                     /locus_tag="IW687_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0949334.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37770..39062)
                     /locus_tag="IW687_000034"
     CDS             complement(37770..39062)
                     /locus_tag="IW687_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0949335.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39232..39861
                     /locus_tag="IW687_000035"
     CDS             39232..39861
                     /locus_tag="IW687_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0949336.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39964..40773)
                     /locus_tag="IW687_000036"
     CDS             complement(39964..40773)
                     /locus_tag="IW687_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0949337.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40828..41697)
                     /locus_tag="IW687_000037"
     CDS             complement(40828..41697)
                     /locus_tag="IW687_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949338.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41698..43014)
                     /locus_tag="IW687_000038"
     CDS             complement(41698..43014)
                     /locus_tag="IW687_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949339.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(43011..44846)
                     /gene="walK"
                     /locus_tag="IW687_000039"
     CDS             complement(43011..44846)
                     /gene="walK"
                     /locus_tag="IW687_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0949340.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44851..45555)
                     /locus_tag="IW687_000040"
     CDS             complement(44851..45555)
                     /locus_tag="IW687_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949341.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45745..46605)
                     /locus_tag="IW687_000041"
     CDS             complement(45745..46605)
                     /locus_tag="IW687_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0949342.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46592..47161)
                     /locus_tag="IW687_000042"
     CDS             complement(46592..47161)
                     /locus_tag="IW687_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0949343.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47542..47614
                     /locus_tag="IW687_000043"
     tRNA            47542..47614
                     /locus_tag="IW687_000043"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47575..47577,aa:Lys,seq:ctt)
     gene            complement(48110..48496)
                     /locus_tag="IW687_000044"
     CDS             complement(48110..48496)
                     /locus_tag="IW687_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0949344.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48493..49014)
                     /locus_tag="IW687_000045"
     CDS             complement(48493..49014)
                     /locus_tag="IW687_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0949345.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(49016..50050)
                     /gene="sppA"
                     /locus_tag="IW687_000046"
     CDS             complement(49016..50050)
                     /gene="sppA"
                     /locus_tag="IW687_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0949346.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50083..51498)
                     /locus_tag="IW687_000047"
     CDS             complement(50083..51498)
                     /locus_tag="IW687_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0949347.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51467..53620)
                     /locus_tag="IW687_000048"
     CDS             complement(51467..53620)
                     /locus_tag="IW687_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0949348.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53878..54114
                     /locus_tag="IW687_000049"
     CDS             53878..54114
                     /locus_tag="IW687_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0949349.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54133..54348
                     /locus_tag="IW687_000050"
     CDS             54133..54348
                     /locus_tag="IW687_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0949350.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55051..55740)
                     /locus_tag="IW687_000051"
     CDS             complement(55051..55740)
                     /locus_tag="IW687_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949351.1"
                     /translation="MVLIKSMNYSGFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDL
                     NHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNE
                     IVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDG
                     QYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQ
                     KIKEKLKESTV"
     gene            55864..57183
                     /locus_tag="IW687_000052"
     CDS             55864..57183
                     /locus_tag="IW687_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0949352.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(57389..57487)
                     /locus_tag="IW687_000053"
                     /pseudo
     CDS             complement(57389..57487)
                     /locus_tag="IW687_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(57502..58749)
                     /locus_tag="IW687_000054"
     CDS             complement(57502..58749)
                     /locus_tag="IW687_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0949353.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(58825..58971)
                     /locus_tag="IW687_000055"
     CDS             complement(58825..58971)
                     /locus_tag="IW687_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0949354.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(59075..59386)
                     /locus_tag="IW687_000056"
     CDS             complement(59075..59386)
                     /locus_tag="IW687_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0949355.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(59388..59585)
                     /locus_tag="IW687_000057"
     CDS             complement(59388..59585)
                     /locus_tag="IW687_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0949356.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(59979..61706)
                     /locus_tag="IW687_000058"
     CDS             complement(59979..61706)
                     /locus_tag="IW687_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0949357.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(61699..62892)
                     /locus_tag="IW687_000059"
     CDS             complement(61699..62892)
                     /locus_tag="IW687_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0949358.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(63079..63723)
                     /locus_tag="IW687_000060"
     CDS             complement(63079..63723)
                     /locus_tag="IW687_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0949359.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(63817..63951)
                     /locus_tag="IW687_000061"
     CDS             complement(63817..63951)
                     /locus_tag="IW687_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0949360.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(63953..66085)
                     /gene="feoB"
                     /locus_tag="IW687_000062"
     CDS             complement(63953..66085)
                     /gene="feoB"
                     /locus_tag="IW687_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0949361.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(66082..66555)
                     /locus_tag="IW687_000063"
     CDS             complement(66082..66555)
                     /locus_tag="IW687_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0949362.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            66956..67180
                     /gene="nrdH"
                     /locus_tag="IW687_000064"
     CDS             66956..67180
                     /gene="nrdH"
                     /locus_tag="IW687_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0949363.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            67339..69498
                     /gene="nrdE"
                     /locus_tag="IW687_000065"
     CDS             67339..69498
                     /gene="nrdE"
                     /locus_tag="IW687_000065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0949364.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            69548..70513
                     /gene="nrdF"
                     /locus_tag="IW687_000066"
     CDS             69548..70513
                     /gene="nrdF"
                     /locus_tag="IW687_000066"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0949365.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(70602..71741)
                     /locus_tag="IW687_000067"
     CDS             complement(70602..71741)
                     /locus_tag="IW687_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0949366.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(72050..72763)
                     /locus_tag="IW687_000068"
     CDS             complement(72050..72763)
                     /locus_tag="IW687_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0949367.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(73017..73718)
                     /gene="nagB"
                     /locus_tag="IW687_000069"
     CDS             complement(73017..73718)
                     /gene="nagB"
                     /locus_tag="IW687_000069"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0949368.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            73952..75484
                     /locus_tag="IW687_000070"
     CDS             73952..75484
                     /locus_tag="IW687_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0949369.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(75675..76877)
                     /locus_tag="IW687_000071"
     CDS             complement(75675..76877)
                     /locus_tag="IW687_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0949370.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(76959..77648)
                     /locus_tag="IW687_000072"
     CDS             complement(76959..77648)
                     /locus_tag="IW687_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0949371.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(77754..78122)
                     /gene="rplL"
                     /locus_tag="IW687_000073"
     CDS             complement(77754..78122)
                     /gene="rplL"
                     /locus_tag="IW687_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0949372.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(78175..78678)
                     /locus_tag="IW687_000074"
     CDS             complement(78175..78678)
                     /locus_tag="IW687_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0949373.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(79088..79777)
                     /gene="rplA"
                     /locus_tag="IW687_000075"
     CDS             complement(79088..79777)
                     /gene="rplA"
                     /locus_tag="IW687_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0949374.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(79881..80303)
                     /gene="rplK"
                     /locus_tag="IW687_000076"
     CDS             complement(79881..80303)
                     /gene="rplK"
                     /locus_tag="IW687_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0949375.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            80646..81314
                     /gene="sdaAB"
                     /locus_tag="IW687_000077"
     CDS             80646..81314
                     /gene="sdaAB"
                     /locus_tag="IW687_000077"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0949376.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            81327..82199
                     /gene="sdaAA"
                     /locus_tag="IW687_000078"
     CDS             81327..82199
                     /gene="sdaAA"
                     /locus_tag="IW687_000078"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0949377.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(82290..83309)
                     /locus_tag="IW687_000079"
     CDS             complement(82290..83309)
                     /locus_tag="IW687_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0949378.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(83482..84369)
                     /locus_tag="IW687_000080"
     CDS             complement(83482..84369)
                     /locus_tag="IW687_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0949379.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(84446..84991)
                     /gene="nusG"
                     /locus_tag="IW687_000081"
     CDS             complement(84446..84991)
                     /gene="nusG"
                     /locus_tag="IW687_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0949380.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(85092..85262)
                     /gene="secE"
                     /locus_tag="IW687_000082"
     CDS             complement(85092..85262)
                     /gene="secE"
                     /locus_tag="IW687_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0949381.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(85286..85438)
                     /gene="rpmG"
                     /locus_tag="IW687_000083"
     CDS             complement(85286..85438)
                     /gene="rpmG"
                     /locus_tag="IW687_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0949382.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            85600..86244
                     /locus_tag="IW687_000084"
     CDS             85600..86244
                     /locus_tag="IW687_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0949383.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(86300..86803)
                     /locus_tag="IW687_000085"
     CDS             complement(86300..86803)
                     /locus_tag="IW687_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0949384.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(86816..86862)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            87400..88365
                     /locus_tag="IW687_000086"
     CDS             87400..88365
                     /locus_tag="IW687_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0949385.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(88464..89636)
                     /locus_tag="IW687_000087"
     CDS             complement(88464..89636)
                     /locus_tag="IW687_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949386.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(89623..90768)
                     /locus_tag="IW687_000088"
     CDS             complement(89623..90768)
                     /locus_tag="IW687_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949387.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(90782..91706)
                     /locus_tag="IW687_000089"
                     /pseudo
     CDS             complement(90782..91706)
                     /locus_tag="IW687_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            91823..92902
                     /locus_tag="IW687_000090"
     CDS             91823..92902
                     /locus_tag="IW687_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0949388.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            92934..93527
                     /locus_tag="IW687_000091"
     CDS             92934..93527
                     /locus_tag="IW687_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949389.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(93637..93864)
                     /locus_tag="IW687_000092"
     CDS             complement(93637..93864)
                     /locus_tag="IW687_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0949390.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(93967..96555)
                     /locus_tag="IW687_000093"
     CDS             complement(93967..96555)
                     /locus_tag="IW687_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0949391.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(96575..97234)
                     /locus_tag="IW687_000094"
     CDS             complement(96575..97234)
                     /locus_tag="IW687_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0949392.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(97283..97786)
                     /gene="trmL"
                     /locus_tag="IW687_000095"
     CDS             complement(97283..97786)
                     /gene="trmL"
                     /locus_tag="IW687_000095"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0949393.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(98007..98873)
                     /locus_tag="IW687_000096"
     CDS             complement(98007..98873)
                     /locus_tag="IW687_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0949394.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            98982..99623
                     /locus_tag="IW687_000097"
     CDS             98982..99623
                     /locus_tag="IW687_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0949395.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(99685..101046)
                     /gene="mgtE"
                     /locus_tag="IW687_000098"
     CDS             complement(99685..101046)
                     /gene="mgtE"
                     /locus_tag="IW687_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0949396.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(101064..101963)
                     /locus_tag="IW687_000099"
     CDS             complement(101064..101963)
                     /locus_tag="IW687_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0949397.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(101965..102762)
                     /locus_tag="IW687_000100"
     CDS             complement(101965..102762)
                     /locus_tag="IW687_000100"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0949398.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(102740..103444)
                     /locus_tag="IW687_000101"
     CDS             complement(102740..103444)
                     /locus_tag="IW687_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0949399.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            103575..104150
                     /locus_tag="IW687_000102"
     CDS             103575..104150
                     /locus_tag="IW687_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0949400.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            104268..104945
                     /locus_tag="IW687_000103"
     CDS             104268..104945
                     /locus_tag="IW687_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0949401.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(105024..106832)
                     /gene="pepF"
                     /locus_tag="IW687_000104"
     CDS             complement(105024..106832)
                     /gene="pepF"
                     /locus_tag="IW687_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0949402.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(107008..108006)
                     /locus_tag="IW687_000105"
     CDS             complement(107008..108006)
                     /locus_tag="IW687_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0949403.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(108232..108885)
                     /locus_tag="IW687_000106"
     CDS             complement(108232..108885)
                     /locus_tag="IW687_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0949404.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        32620 a        22570 c        18651 g        35354 t
ORIGIN      
        1 gcagatgcaa gcttttgaac accgtttaag gaagaggttg taaaatcagc ggtttgacct
       61 gagtattaga gatacaaaac ggaaaaatag cttctcatat tttgccgttt tttgagctaa
      121 taccgaagga cagctgattg aacaccgttt attcggaaaa agggactgtg acacgacttt
      181 tgtcacagcc cctaaaaaaa ataataaatt tgtactattc tatgaagctt cttcttcaga
      241 aaactgactg ttataaagat ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt
      301 tcctgtttct acgatcgaac catgattcat cacaataatg ttgtctgcat cgcgtattgt
      361 agacaagcgg tgagcaacaa cgaaacttgt ccggttttcc aataggcggt tcatcgcttt
      421 ttgaatcagt atttctgttc gtgtatctac actagatgtt gcttcatcaa ggatcaggac
      481 atctggattt gctaagaatg cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat
      541 attgcttgca tcctcgttta atacagtgtt atatccttct ggcaatttac gaacaaaatc
      601 gtctacgtga gcagctttag cagcctcaat gatttgttcg tctgttgcat cgtcgttccc
      661 atatttgata ttgtcataga tacttcctgt aaacagccaa gtatcttgca gtaccattga
      721 aaactgtgcg cgcaattcat cacgtgaaag atcacgggta tctacaccat cgtaacgaat
      781 actacctcca gaaacatcgt agaaccgttc aagaaggttg atcagtgtcg tcttccctgc
      841 accagtcgga cctacaatag cgaccatctc accaggtttg acatccaagt taaaatcagt
      901 cattagcaag ttatctggtg tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg
      961 tgtatctttt tcttctggaa ggccagattt ttcattggac atctcttctt catccaacac
     1021 ttcaaatacc cgttcggctg aagcaactgt tgattggata gtgttcatca aacttgcaag
     1081 ttgagtgatt ggttgagaga actgtgttgt atattgcaag aacgcttgaa catcccctaa
     1141 tgtcatgttc ccatttgcaa cttttactcc gcctagtaca gcgacgaaca catagcctag
     1201 atttttaacg aaattcatca acggcatgat gattgccgaa ataaattgag ctttccaacc
     1261 agcggcatac agattctgat tttctttttc aaatacttcc tggtcttttt cagtatgatt
     1321 gaatgtttta acaatggtat gaccaccata tgtttcttct acttggttat tcaataaacc
     1381 taaacttttt tgctgatctg cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat
     1441 gatcaagctt aaagggatca tcaataacgc gactaacgtc aattgccagc tgattgaaag
     1501 catcatcacg atgaccccaa tcaatgtgac tgcactagtt acaaattgag tcaaattttg
     1561 ctgcagcgtg cttgcgatgt tatccatatc attgattgct cgagacatga tatccccatt
     1621 tgtatgtgta tcataatagg aaatcggtac acgattcatt ttggcttcca aatcatgacg
     1681 taattcataa actgttcgtt gggaaacacg tgtcataata aattgctgta aaaagttgaa
     1741 tacagcagaa atcaaataca tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa
     1801 gtctatcgga aattttgtta cttgttggcc ttgttgcatc tgttgagcgc cagccattac
     1861 acctttgaat atttctgtgg ttgctttccc caagaccttt ggtgtttgga tttggaagat
     1921 cacggctgct attgctagta caaatacagc gatgatcgca accatacgtt tagacatata
     1981 gccaaacaat cggcgtaccg ttccccagaa atttttaggc ttttcacctt ttacaccgag
     2041 atttctgcca ggtccaccat gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt
     2101 tgttttactc atgctaagtc ctcctctctt agttgtgaat gaacgatttc ttgataagtt
     2161 tcgtttgttg ctaatagttc ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc
     2221 acaaggatca tatctgcatc cattactgta cttactcgtt gagcaactaa aacggtgata
     2281 ctttctttca tattcgtttt caatgcttgg cgaagattgg catccgtttt gaaatccaac
     2341 gctgagaagg aatcatcaaa cacatagata tctgctgttt tgactaatgc ccgtgcaatt
     2401 gccagacgct gtctttgtcc accagagaag tttcctcctc cttgttccac atggctatct
     2461 aagccatctt ccatctctga aacaaaatct ttagcttgtg cgatttctaa tgctttccaa
     2521 atcatttcat ctgttgcatc cggcttacca tactgcatat tgtctcggat cgttccagag
     2581 aacagaactg ctttttgagg aacaaagccc attaattctc gcaaattata ttgagtcatg
     2641 tcactaatgt ccgttccatt tatttggatc gaaccagatt cgatgtcata taatcgaggg
     2701 atcaaattga ctaaggtcgt tttacctgaa ccagtccccc ctatgattgc gacgatctcg
     2761 cccgacttgg cttggaaatc aatatcttcc aatgcaagtt tttctgctcc atgatatcga
     2821 taattgacat gattgaaggc caatgttgcg ttttttccag caaaagaaac tgttttagga
     2881 ttttcagggt catggatacc gattttttca tccaacactt cattaatacg gtcagctgaa
     2941 gcttgtgcac gaggcaccat gataaaaata gcggaaagca tcataaagct gatcaagatc
     3001 tgcatcgcat aagtcataaa tgcaatcaga ttaccgactt ccaaagtcat atctgctatg
     3061 tagtgtccac caaaccaagt gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt
     3121 ggcatcatca atgcgacgat cgtattaact ttgattgccg tatttgtata atctttattc
     3181 gctaaatcaa aacgattttc ttcaaagcgt gtcttatcga atgctcgaat cactctgacc
     3241 cctgtcaatc cttctcgaaa taccaggttt aaacggtcag ttttcttttg catacttttg
     3301 aataatggaa cagcaaagta catgattccg ccgatcaaaa taatcattac tgggatgaca
     3361 actaagaaga tttttgtcaa ctgatgatct ttttgataag caagtatgct cgcgccaatc
     3421 aacgtgattg gtgcattgat catcatacgt aaaaacattt gtgtaaccat ttggatttga
     3481 gtcacatcat tggtcgttct tgtgatcaaa gaggccgttc catatttatc aaacgcatct
     3541 tttgtcagac tctctgattt tttatagaca tccgtacgca attgcttacc cagtttctgt
     3601 gattcacgag tagcaaaaaa tgtatttgcg atagcagcta agatactgat caaagaaaag
     3661 ccgatcatca caaaacctgt atgccaaata taatcgacat ctccttgcgc taccccttta
     3721 tcaataatat tggaggtaag tgtcggcaaa tataaatctg ccagtacttg gatcaccatg
     3781 aaaactgcag ctgcaattgc tgaagtcaag gagatccgtt ttacgatttt tagcatctag
     3841 ttcccttctt tctatttata tactccgttt ttcagccaag aaagtttcaa attgaattca
     3901 ctgattgctt gctctactga gtaatcttca gatgttgaaa ataagcgata cgaatgcgca
     3961 attgttgtaa atacagtatt catcactaat tggatcagta attccaattc atgatcattt
     4021 tttactttca gcttcgtccg atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt
     4081 tctttatgtg gatcgtgagg cgttttttgg cgactcgtat gaggagcgac ttttcttgag
     4141 gcacggtaat ccatgttcat aaacaagtta cgataaaaag aagcattctc gccttccaaa
     4201 atctcataaa tcattttaga aaaataggat tcgaatcctt tgaatagatc accatcggat
     4261 tctttcatta gttgtaacat atctttagag ctgtcacgtc tcattgtctc aaagtaataa
     4321 aaataaagat cctctttatc ttcaaaatac tgatagaaac tgcctctagg gatatccgct
     4381 aatttgacga tattagcaat cgaagcttct tttaagggta ctctagaaaa ctcgattctc
     4441 gcagcctcga ttagtctttt ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc
     4501 atatggatcc tcctttcttt caagatgaca cattgtcata tgacgctgtg tcattataac
     4561 acctcctacc tgttttgcaa gacatttgca tagttttttt cattatcatc cgatacatta
     4621 aaaaagtgat aagaggaatc cttccatttg aattaatgag agttgcccat taaaatagcc
     4681 acatactaaa caaaggatga gataaatggc ccaatcaatt agtgtccaag acttcaataa
     4741 attgcctctc gatgctgaca gcatcgtttt agatacaaga aataccttag attacacaaa
     4801 aaaccactta agccactcgc tttctatccc cgctgctatc ttgccaagac aattaaaaaa
     4861 tctagatcca aagaaacttt aatatattct aagctataca ggcaggcgat cagaagcatt
     4921 agccgatcaa cttactgcta atggttttcg tgctgtttat atcattggtg ggatggaagc
     4981 tcttcagtcg agtgttgctt gaatattgct tgttagattc aaaagaggct gtgacaaaaa
     5041 atcgtgtcac agcctctttt ataccctgtc tttttttagt tatttattct tcaaccttat
     5101 caatatgact tgatagcctt ttctttttct catagttata acaatcatag caataaaagg
     5161 agaaggtaag catatggcat ctgttttagt agtcaaaggt catcccctaa ctgcagaaga
     5221 atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt tataaagaaa atcatccaga
     5281 tgatgaagtg actgttttgg aactttatcg cgatgatatt ccggaaatcg atgaagaatt
     5341 attatcaggt tgggaagcat tacgtgctgg tgcagaattc acttccttat cagaaagtca
     5401 acaacaaaaa attgctcgtt tcaatgaatt gactgatcag ttccttgctg ctgataaaat
     5461 cgttatcgca aacgccttat ggaatctaaa tatcccaaca aaattaaaag cttggttcga
     5521 taccgtcaat gtagctggaa aaacgttccg ctatacagaa aacgggccag aaggattagt
     5581 gacaggtaaa aaagcactgc acatccaatc taatggcggt gtatacaacg gacaagactt
     5641 tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag tagatcagct
     5701 attcatcgaa ggaatcgatt atgaccctga ccgtgcagat gagttgatgc aaaatgcatt
     5761 agataaagct gctgctttag gtaaatcatt ttaaaaattg agataaatgc atacaaaaga
     5821 gacagaggaa aaaatcttca aagcagcatt tgtaccttgg atattttccc atctgtctct
     5881 ttttgttatt ctttctgctt tttcaacgga ttttcttcat aaagagaaga gattgttgtg
     5941 taccattcaa aaacatgagt aatcactact ttagctgctg cataaacagg aatccccaag
     6001 atcacaccga tgattccgaa caatttccca gaagtcagca gaactaataa aatcgttact
     6061 ggatggattg ctaactgatt ccctagtact aatggagaga tcaagcgtcc ttctattgtc
     6121 tgctctactg caaaaacgat cagtactttg atgatcatga atggacctgc tacaatggct
     6181 aaaaagatag ccggaatcat cgctaaaaat gatcctaagt aagggatcag gttcaaaaaa
     6241 cccgcaataa tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa accaatcata
     6301 aacatgattg ccacagcaaa agccacagtt agttgtcctc ttatataaga agaaacttga
     6361 ctattcattt cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg
     6421 acaaaataag gtgctaattg tttaccgtct ttcaatagat aaaataagat aaacggcatc
     6481 gtcagcagag ccactaaaat agtcgctacc gcgccgacaa aacttccaag actctgaact
     6541 gtactcttag atatatcttg gatcatatcg cctgccgaat tcatgaactt atcacctgct
     6601 gtttccaact gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc ggttaattta
     6661 ttatcaatcg tatctacata ttgagggaag ttactgatga agctcactgt ttgttcctga
     6721 atcttaggga tgatcactac gctcccccat gcgattagtg caacaaccaa aacaaacagc
     6781 ccaatgatac tgtaaagcct tggtactttt cgcttttcaa agtaatcaac taccggattc
     6841 atcaagtaat acaagattcc tgctaaaatg ataggcagac ctacgattgc taaaaattgc
     6901 cagattggtg caaacaaata tgaaactttt gtaaataaaa agacgatcag caatattaac
     6961 aatacaacaa gtaaggctgt gactacttga ttatttaaaa accagcgcca aaaccacgat
     7021 accggtctgt tcttcttatt ttttccttcc ataattcctc ctgttagtta taaaaaattt
     7081 ggcttcccaa accaatagca ggcagtctat gcggatgaag atagattcca tttgcaattt
     7141 gtccttcttc tggacaagca tcaaaaatca atgatacgtg agctactgtc tctaaattcc
     7201 cattggccat cggaccgaca taatcgatcg tatatacctg atcatcaaaa gcaattgttc
     7261 cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc gaatattttc
     7321 gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc ttgtttattg
     7381 cttctgtccc aatttctgta atctttgcct gaatcattgc actctctcct ttaattgctt
     7441 tcaaaaacag tataacacat tctcattctt tagccgtgtg gaataattat gtgatagaat
     7501 aaaaacgaaa actatttcat tacaattagg aggaatcagc tcatatgatc gaaaagataa
     7561 ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac cttgatacag
     7621 aaaaagaaga attagccaac ctttcattag ctgttgaaga aaatagccct acctatgtag
     7681 caaaaagtaa agctggcaat ctttacacag ttactagtgt ggatggatta ggtggtgccg
     7741 gtgcgtacga taaggatttt cattttttga atgcggtaac agagtctggc gctcctttgt
     7801 gttatgtagc ggttgatgaa gcacgtcaat tactatacgg ggcgaactat cataaaggtg
     7861 aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc
     7921 atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc
     7981 tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg
     8041 atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag
     8101 gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag
     8161 acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga
     8221 aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt
     8281 caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg
     8341 cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt
     8401 ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca
     8461 ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa
     8521 aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga
     8581 atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat attcctttct
     8641 ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg
     8701 atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata
     8761 ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa
     8821 ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt
     8881 aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct
     8941 atcataaagg taatattaca tttatattag ataactctag tttttattgt tatcaaagat
     9001 ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct
     9061 gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca
     9121 agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca
     9181 aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcttaagc
     9241 attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat
     9301 ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga
     9361 tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa
     9421 tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt
     9481 ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc
     9541 tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac
     9601 aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg
     9661 cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc
     9721 ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat
     9781 tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa
     9841 ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt
     9901 tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt
     9961 ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga
    10021 aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg
    10081 atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg
    10141 aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc
    10201 atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat
    10261 gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta
    10321 tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag
    10381 ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacgc
    10441 catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg
    10501 agaaagcata ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc
    10561 accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa
    10621 cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta
    10681 tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc
    10741 taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg
    10801 gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt
    10861 gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat
    10921 caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat
    10981 cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag
    11041 caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact
    11101 cattttcata taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc
    11161 taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca
    11221 ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga
    11281 tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact
    11341 gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata
    11401 tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt
    11461 agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct
    11521 ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt
    11581 ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa
    11641 tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc
    11701 attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa
    11761 tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt
    11821 accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc
    11881 ttcctgagtc gatcccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc
    11941 ttcttctgga aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac
    12001 agcatattta tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat
    12061 tttttctata ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac
    12121 tacgatgtaa tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa
    12181 ctgggcatcg cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc
    12241 tacttttgtg cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg
    12301 tcccacataa gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt
    12361 ttgtaccata cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac
    12421 aagctctttt actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc
    12481 agaagctttt tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa
    12541 atctggtgta agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg
    12601 caaatcatct attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat
    12661 agcatcaatc aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa
    12721 tttctgcaat tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga
    12781 gcagagtagg ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt
    12841 tcgagagcaa aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt
    12901 gataataaga ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt
    12961 cttaaaacta agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc
    13021 aactaatttt gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg
    13081 atgaccgcca cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt
    13141 ccataacaca cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac
    13201 ttcattgatc agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc
    13261 tcctgctttc ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg
    13321 agaaacaacg attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga
    13381 ttccaagatt gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt
    13441 atctttccct cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc
    13501 ttccccagca aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg
    13561 tttcattcca tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa
    13621 gaaacggcga ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt
    13681 ttcttcataa tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc
    13741 gacttcttcg tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc
    13801 aacagtccaa ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt
    13861 tctcatgttg tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc
    13921 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    13981 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    14041 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    14101 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    14161 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    14221 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    14281 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    14341 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    14401 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    14461 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    14521 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    14581 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    14641 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    14701 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    14761 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    14821 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    14881 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    14941 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    15001 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    15061 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    15121 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    15181 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    15241 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    15301 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    15361 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    15421 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    15481 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    15541 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    15601 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    15661 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    15721 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    15781 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    15841 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    15901 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
    15961 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    16021 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    16081 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    16141 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    16201 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    16261 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    16321 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    16381 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    16441 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    16501 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    16561 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    16621 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    16681 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    16741 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    16801 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
    16861 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    16921 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    16981 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    17041 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    17101 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    17161 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    17221 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    17281 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    17341 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    17401 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
    17461 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    17521 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    17581 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    17641 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    17701 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    17761 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    17821 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    17881 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    17941 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    18001 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    18061 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    18121 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    18181 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    18241 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    18301 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    18361 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    18421 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    18481 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    18541 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    18601 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    18661 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    18721 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    18781 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    18841 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    18901 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    18961 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    19021 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    19081 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    19141 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    19201 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    19261 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    19321 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    19381 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    19441 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    19501 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    19561 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    19621 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    19681 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    19741 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    19801 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    19861 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    19921 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    19981 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    20041 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    20101 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    20161 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    20221 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    20281 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    20341 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    20401 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    20461 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    20521 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    20581 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    20641 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    20701 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    20761 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    20821 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    20881 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    20941 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    21001 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    21061 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    21121 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    21181 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    21241 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    21301 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    21361 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    21421 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    21481 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    21541 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    21601 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    21661 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    21721 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    21781 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    21841 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    21901 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    21961 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    22021 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    22081 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    22141 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    22201 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    22261 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    22321 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    22381 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    22441 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    22501 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    22561 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    22621 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    22681 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    22741 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    22801 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    22861 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    22921 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    22981 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    23041 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    23101 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    23161 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    23221 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    23281 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    23341 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    23401 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    23461 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    23521 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    23581 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    23641 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    23701 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    23761 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    23821 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    23881 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    23941 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    24001 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    24061 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    24121 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    24181 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    24241 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    24301 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    24361 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    24421 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    24481 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    24541 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    24601 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    24661 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    24721 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    24781 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    24841 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    24901 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    24961 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    25021 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    25081 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    25141 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    25201 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    25261 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    25321 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    25381 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    25441 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    25501 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    25561 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    25621 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    25681 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    25741 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    25801 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    25861 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    25921 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    25981 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    26041 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    26101 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    26161 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    26221 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    26281 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    26341 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    26401 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    26461 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    26521 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    26581 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    26641 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    26701 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    26761 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    26821 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    26881 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    26941 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    27001 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    27061 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    27121 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    27181 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    27241 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    27301 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    27361 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    27421 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    27481 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    27541 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    27601 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    27661 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    27721 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    27781 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    27841 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    27901 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    27961 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    28021 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    28081 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    28141 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    28201 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    28261 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    28321 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    28381 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    28441 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    28501 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    28561 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    28621 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    28681 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    28741 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    28801 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    28861 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    28921 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    28981 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    29041 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    29101 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    29161 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    29221 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    29281 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    29341 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    29401 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    29461 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    29521 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    29581 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    29641 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    29701 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    29761 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    29821 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    29881 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    29941 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    30001 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    30061 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    30121 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    30181 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    30241 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    30301 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    30361 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    30421 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    30481 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    30541 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    30601 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    30661 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    30721 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    30781 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    30841 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    30901 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    30961 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    31021 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    31081 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    31141 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    31201 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    31261 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    31321 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    31381 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    31441 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    31501 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    31561 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    31621 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    31681 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    31741 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    31801 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    31861 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    31921 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    31981 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    32041 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    32101 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    32161 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
    32221 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    32281 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    32341 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    32401 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    32461 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    32521 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    32581 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    32641 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    32701 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    32761 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    32821 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
    32881 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    32941 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    33001 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    33061 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    33121 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    33181 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    33241 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    33301 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    33361 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    33421 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    33481 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    33541 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    33601 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    33661 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    33721 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    33781 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    33841 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    33901 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    33961 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    34021 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    34081 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    34141 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    34201 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    34261 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    34321 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    34381 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    34441 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    34501 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt
    34561 ttaatagata aaatattata actattaaaa ataactattt tcattataat tacacacaaa
    34621 aaagagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaaca
    34681 gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    34741 tttccgtgaa tttcttctta tttcttggag ttaaacgctt ctgttccagc ctctttttat
    34801 ctgtctttca ctaatgatca gttgtagata agcaagttgc tttttcttac atcataccgc
    34861 ccatcattga tggatccatt gcaggagctg ctggcgctgc tggttctggt ttatctgcaa
    34921 cgactgcttc tgtcgttaat aacaaggcag aaacggaagc ggcattttgc aaggcagaac
    34981 gtgttacttt agttgggtct acgatacctg cttcaaccat gtttacccat tctccagtag
    35041 cagcattgaa accagtgcct agttccacgt tcttcaattt atcaacaatc actgatcctt
    35101 cataaccagc gttttcagcg atttgacgaa ttggttcttc taaagcacga acaacgatct
    35161 tgatacctgt tgccacgtct ccttctgctt ctacagcaga aactttgctg ataacgttca
    35221 ctagagctgt accaccacct gagaccattc cttcttctac tgcggcacgt gtagcattca
    35281 atgcatcttc aatgcgtaat tttaattctt tcaattctgt ttcagtagct gcaccaactt
    35341 taacaacagc tacaccacct gctaatttag ctaagcgttc ttgtaatttt tcgcggtcaa
    35401 aatcagaagt tgtttcagca atttggtttt tgatcaattg cacacgagct tcgattgctt
    35461 ctttttctcc agaaccttct acgatcgttg tattgtcttt atctacaaca actttagaag
    35521 catttcctaa gttttcgatt gttgcatctt tcaattctaa acctagatcg tcagtaatta
    35581 ctgtaccacc tgtcaaaata gcgatatctt caagcattgc tttacgacga tcgccgaaac
    35641 caggagcttt tacagctact acgttgaatg ttccacggat tttgttcaat acaagagttg
    35701 gcaatgcttc accatctaca tcgtcagcaa taatcaacaa tggacgtgat tgttgcaaga
    35761 tttgttctaa caatggcaga atatcttgga tgttagagat ttttttgtct gtgatcaaga
    35821 tatatgggtt ttctagaaca gcttccattt tgtcgttatc tgttaccata tattgtgata
    35881 agtaaccacg atcaaactgc ataccttcta caacatctag ttctgtttcg atcccttttg
    35941 attcttcgat tgtgatgaca ccgtcgtttc caactttttc cattgcatca gcaatcaaat
    36001 gtccaacttt atcagaaccc gaagaaacag ctgctacttg tgcaatggct tcttttgaat
    36061 caacaacagt tgagatatta tgcaattctt cgactgctgc ttttgttgcc aattcgatcc
    36121 cgcgacgaat gcctagagga ttagcacctg cagttacgtt ttttaaacct tcgcgaacga
    36181 ttgcttgtgt cagaacagtt gctgtcgttg taccgtctcc tgcaatatca tttgtttttg
    36241 atgctacttc tgatacaagt tttgcaccca tgttttcaaa atgatcttct aattcgattt
    36301 cttttgcgat cgtcacacca tcatttgtga tcaatggaga gccgtaagat ttttctagta
    36361 cgacatttcg gcctttaggt cctaatgtta ctttcactgt atctgctaat ttatctaccc
    36421 cgcgtaacat agctgcgcgt gcgtcttctg caaatttcaa ttcttttgcc atgattgatt
    36481 cacctcattg attatttttg attatttttt tatcttccaa atttactcaa caatcgccat
    36541 gatatctttt cctgcgacga tcagatattc ttttccttcg tattttactt ctgtaccggc
    36601 gtatttttcg aacataactt ggtcgcctgc ttttacagcg gcaggaactt tttcaccgtt
    36661 ttctaacaaa cggccttcac cgactgcaac aactgtccca gtttggggtt tctctttagc
    36721 agcagaagct aaaacgattc ctccgacagt tttttcttct tcttttgcga cttcgataat
    36781 gacgcgatca cctaatggtt ttaacacgat aaatccctcc gataactctt tttttaatct
    36841 gttagcactc ttaaccttcg agtgctaacc tacattttta ataatactca ttctccctct
    36901 tttttgcaag cttttaaatc aaagaaaaga aattttattc tgacagctgc ttattgaaat
    36961 tatgatataa ttgaaatcaa tcgatataag cttgccttgc atgcttgtta tcccttaaaa
    37021 ggaggaatta catgtcttta aaaaaatata gcttcctcag cattttttgc tatggtctaa
    37081 tttttatctc acccttgatc ctagcttcga taggacttgt aaaatctacg tctgaattga
    37141 tcacagcaac tgcagtcatg tatatactag gcgccgttgt cctagctatt ttttatttta
    37201 agcaacgtga acccttagcg atcgaatcag cggtaaaaaa agcttctgct ccaaagattg
    37261 ttatttatgg attggttgga atctttgtcg cattgattct gcaaagcatc gctgtcatgt
    37321 tagaatctgt attgtttggc aaagcaaccc cttccgagaa tacacaaaat atcatccaga
    37381 tgatcatgga agcacccgct tttattttgg cgacaactgt tgctggaccc atcatggaag
    37441 agttcgtatt ccgccgcagt atactgggga tcatcagtcg atattcaaat ttttgggtcg
    37501 gagcagtgat cagctctttg ctgtttgcgt ttgctcataa tgatggtcat ctgctaatct
    37561 actttttcct aggtttcttc ttcagcctag agtacaaagc aactggtcgg atctggacat
    37621 cgatgatcac acacgtagga atgaacacat tagttgttct tgttcaatta gctgtccaaa
    37681 aagggctagt ataaacatga ctataaatac tcctgtcatc ctagattggt gattttccca
    37741 atctaaagcg acaggagtat ttttgttttt tagtcgttca tcttttctgc tgctccatca
    37801 ttttttgtct gtctttcctt tttgcagttt gcagaatcta tttttttcaa aaatgggaaa
    37861 taaatgaaaa aactaattga caacacgata ccttgtaaaa tcgctgcagg cactccgcct
    37921 actaagaaac ccgagaggat cggcggtgtt gtccaaggga cttggactgc tgtaaataaa
    37981 ggaacgaatc caaaatataa agcggaatag gtaatcaatc ctgaaacgac cggtgcgaaa
    38041 ataaaaggaa cagccatcat cgggttcatc acgatcggtg tggaaaaaat aattgattca
    38101 ttgatgttga aaaaggctgg taaaagagca agatttccta attctctgta tttcttcgat
    38161 ttcgcaaaaa ccgtcataaa aaatacagcc cctaacgtca atccagcacc cgtaaccgtc
    38221 atgaactgat cgagaaattg ctgagtaacg atatgtccgc catttgccac cgtcaactct
    38281 tgtcctgagg ctaagatttc tgcatttgct aatgaattcg cttgcaagac gggactaacc
    38341 aacccattga ccatcgcaga tccatgaaga ccaaataacc ataaaaacgg aacaaaaaaa
    38401 cctaataaaa gcacaccaaa caacgtgtct atgaacgatt gaatgggaag ctggacatat
    38461 gtccatactc cttgaacaac agtcattctg aacaaatgtt gaaaaaccgc tgaaccgata
    38521 agcgtaaaag tgactataac aatgattgga atcaatgaag taaaagactg ccaaacattt
    38581 tcaggtatct tctctctttg cttccaccct ttcgcaagtc ttactaaacg tgcatagccc
    38641 catccgatga tgagcccagt tgtaactgct cctagtatcc cttcagcacc tgtccagaca
    38701 gtatctatct gagtatttag tggctgaata agcagtagat aagcagacaa ggctgttatg
    38761 ccgactggta agcgatcgat gtcttcttct tttgcataag cgtatgcaat ggcaaaaacg
    38821 atccaaaagg ctatcacatc aaatgtagcc tggtatactt gtgtaaaata catagaaaat
    38881 cctgttttgt ctatccactg agcgatgtaa tccacaggaa aatgagcaaa taagaaaaac
    38941 agcgagccaa caataattat tgggatcgtg taagtcattc cattacgcaa agcagttaag
    39001 ggtctaagat tcacaacaga tgttatcatt ggtaaaaagg cacgatctaa ccactctttc
    39061 atttcttatc tccttcaact tctattttta tcaatcagtt ttcttaattt aactataccc
    39121 aatgatcacg aaccatgaaa ggaatgcgca ctgagctttt caaaaatcgg attctttgac
    39181 gaatattcag caactgctta caatggacag gaataaaaaa ggaggttttt tatgaaaatt
    39241 gcactcattg gagctactgg ccatgcaggt tcatttattt tagacgaagc attgtccaga
    39301 gatttagacg tgacggcaat cgtgcgtcat ccagaaaaat tgccatcaga tgttccgttt
    39361 atacaaaagg atttatttga gttgacaact actgatcttg catcttttga tgttgtcgtt
    39421 gatgctttca acgctcccaa aggaaaagaa gaaatgcatc aaacttcttt gtcccatttg
    39481 attgagatgc ttgaaggaac cgatacacgc ttaattgtcg ttgggggtgc ctcttcgtta
    39541 ttcttagata cagaaaagac gactcgtatg attgaccaag tacctaaaaa tgcacccttc
    39601 tatccaacag ctttcaatat gtacgaagga ttgttaaaat tgaaacaatc taaaaatcta
    39661 cattggactt atatcagtcc agcttcttta tttgatccac atggcaaacg tacaggtact
    39721 tatttgttaa gtgatgatta tttacaaaga aacgctgcta gcgacagtac gatcagcatg
    39781 gcagattacg cgattgcatt agtagatgag atactggatg agaaacatga aaagcagcac
    39841 atcagtgtag tcagtcgata atacggttat ttttttaata tgctaaaaaa ataaaaatag
    39901 aagataaaac caaccctgat tgattttatc ttctgctttt tatattttta ttgattgaac
    39961 tgtttaaatc gaaaatagct cagttgcttc atctggatct gtatcataga tttcaaaatc
    40021 atgtccaacg ccaaaatctt tttctttcaa aacattttcc attgttaaat gagcaagttc
    40081 tttcatattg ttttccttgc taagatggcc aagatagatt cgtttggtcc gattaccgat
    40141 cacatcagtc atgacaagtg caccgtcttc gttggaaaga tgtcctctat ctcctaaaat
    40201 acgctgtttt aaattccacg gataaggtcc catgcgtaac atctcaagat catgattact
    40261 ttccatcaaa taaccatcgg cattttcaat gacacctcgc atataatcac tgcaatatcc
    40321 ggtgtccgtc aacatgacga atgaccgatt ttctttatgg aaacgataaa attgcggagc
    40381 cgctgcgtca tgggagacgc cgaaactctc aatatcgata tcgccgaatg ttctgacttt
    40441 ccccatctca aatagatgtt tttgttccac tggtacattt ccaatcagtg gttccatcgc
    40501 ttcccaagtc tgctgattgg caaaaacatc caaatgatac ttacgagcca agacacccac
    40561 gccatgtata tgatctcgat gttcatgcgt cacaagtatt ccatctaatt ggtccggtgt
    40621 ccgatcaatt tgagccatca aggaagtgat ttttttaccg ctaagtcctg catcgaccaa
    40681 aagccgtttt ttttcacttt cgatataaag tgaattaccc gaacttccac ttgctaggat
    40741 actgatttta aaagctggtt tttcttcagc catttgactc ctacttccct tcttactttg
    40801 taaacttttt tcattatact cgctttttta ttgatcttcc acttttggta cagtattgtt
    40861 tgtcacgatc gtattgctga aagcgttgat gctctcaatt tggaccactt tatcgcttgt
    40921 ttcgatcgct acatgccaaa caggaacata cacatttttt tcccggactt tcagaataca
    40981 ggaataagct agctgacgcc ataaaatttt tgatcctctt ggtattttgt tattgatgta
    41041 caatgtattg attgcttcct cttccgtata cagttccgtc ttttcgcgca gctgctcgat
    41101 atcagataaa tgcgtctgca tgtatttcgt gatgctataa tcattatcgt ttttttctaa
    41161 agaaagaacg atttttgctg tatcatcatt gaatgggatg ccttcatact cttggcttgc
    41221 ttggatttct agtggttctt cttctaaatg agaaaagttt ttcgtgtaca cataatcatt
    41281 accgaataaa atattgtctt tgttagataa aaaatcttcc aaggaactac taattttatc
    41341 tggatcgatc acgttctttt cagcatctga actggataaa gatttcgtca aaacatcacc
    41401 gtctaaaaac acaccacttg ccagcaagcc gcttttacca gccttgcgat aattcgctaa
    41461 tgaagtgcta agattggtct gctccgcgct aagataatag ccttgcttgt tctcagacga
    41521 aatcttcccc gtgtaagtga tatcgtcgct agccagacgt tgttggattg attcagtctg
    41581 attagagcga atgacattgt tttcttggta taccgcgtca tgataactgc tgaataaaaa
    41641 cacattcaac cctaaaaaag cgacgaaaaa tatccattcg atttttttga aatccaatta
    41701 attcgcctcc aatccaggta atttttccag aaggtcgtta gctgaatacc agttatcttt
    41761 atatttcaca taccattcag gtaagagatc aacgacacga ttcgtttctt tgacattttg
    41821 ccatgtatag ccaacgatca ctgaaccaat cttgttgcta tctgctccct tagcttttaa
    41881 ctgctggatg atttcattcg tacttgcaag ttctacttct tcgtctgaag gtatcggcac
    41941 ttgaatcgta ttcaaactgg tttggatatt cacttgcaca gaaccattcg tttcaccgcc
    42001 aacttccaca ccaatctgtc ctctgctgtc tgtcccaaaa accgggaagc cttccacgaa
    42061 tatccgataa tcgatcgtat tgtcactgcg gtcaaaatat ctgaggttcc ctaaagaact
    42121 gcctaatttg ctaatatagg ggaaactttc agaaaataca ctagcatttt cttttttctc
    42181 tggcagctct ccacgaaaat caactacttg cgtttccgaa tggatagaca ttgtttcatt
    42241 cccaccgtaa aaaatcaatt cattgctttc atcattattt ttcacgctat ctggtttctg
    42301 gaaaaaagca tttctaaaaa gtgtataggg ttgctgtgac aggatataac tatacttctt
    42361 cagtttgatc gaatgtttcg tagcatattg cgtgtcgatc gtcgatggtt ctgttgtcat
    42421 ttctgtccat tttgtgtctt ttgctgttaa ttctttattg atatctgtcc aattcaacga
    42481 aatatttgct tcatatacga gatggttcga ataattcaaa aatcggattt ttttatttcc
    42541 ttgatcgaat tgtattctag taaaatacat attttcgctt tcttttgcca agttcgacac
    42601 atctaaatct aaatcaaatg cttccttgta ttctcttagc aaaaaaggcg cattataggc
    42661 caactcaaaa ccagagttca gatttttcac ctgttcaaac tgtgatttgt ctcctgaaac
    42721 tacttcagaa agctttccaa atcttgcttt atcgatgatc aattgcaatc ggtacacgag
    42781 attttcactg ttggtttctt ggatcattcc cgaatggtgc catgtaacat gaagcggaag
    42841 aaagacttcg gaagtttttc gattgctttg agactcgatc atttgagtgt ttgtctcatc
    42901 tacgttgatt gaactttttc ctgctgggct cagccagatt gcataagaga agtatatgct
    42961 taacgcaacc atcaaaatga gtcctatacg aacgactttt tcagagattc tcattcccac
    43021 cattcctcct cataaggttc atatggcagc gagatataaa aagtcgaccc ttttccttcc
    43081 ttgctctctg cccaaatagc gcctcgatgc gccttaatca cttctctaga aatagctaat
    43141 ccaagtcctg tcccaccttg tgctcttgca cgtgctttat ctactcgata gaaccgctcg
    43201 aacactcggt tcagatcttt tttaggaatc cccaatcctt gatcggtgat acttaaaatc
    43261 acgttattat gcgtttctaa taagcgacaa gtaatcgttc ccccatctgg tgaatacttg
    43321 atcgcgttat tcatgatatt atcgactact tggatgattt tatctgtatc tacttcgacc
    43381 caaagatctc tttgcgtaaa ttcacgacgg atcttataat ttttttcctg atttcccacc
    43441 atcatatcaa accggtcaag aacaaaattc actaactcgt tgaaattgac atattctaat
    43501 tgaagctggg tattacctgt atccatgcga gatagattta agaggtcatt gatcattcga
    43561 atcattcggt ctgtttcatc tagcgttacc ttcaaaaaat ttggtgctac ttcctcgtct
    43621 ttccacgcac cttcgctcaa tgcttcgata taactacgca tacttgtcaa cggtgtacga
    43681 agttcatggg aaacattaga gacgaattca cgacgttccc gttctgtttt ttcttgttct
    43741 gtcacatcat gcaagacagc tactaatccg ctgataaaac ctgattcgcg acggatcata
    43801 gaaaagtcta ctcgcaagat catctgatca ctttccaatg tactggttga acggtcaagt
    43861 accatttcgt ttggatcttc taataatttc cgcaacgtat attcttcttc gatatctaaa
    43921 agtgtcaaaa tcgattgtcc gattgcttcc tcgtttttga tatctagcaa ggaaacagcc
    43981 atatcattga tcgtaatgac tttacccctg cgatctgtcg caatgacgcc gtctgtcata
    44041 tgggatagca cgctatccaa acgattgcgt tcagcttcca ttgtatcttg agcttcttca
    44101 atacgttcag ctaactgatt gaaggtatca gccagctgtc caagttcatc ttgtccatgc
    44161 actttgactt tccgactgta gtctcctcta gcgattcgaa tggcttgttc tctcatttca
    44221 ccgatcggct gtgtaatcga acgagcaact aaaacagata cgatcattga aataaatgcc
    44281 gcaattagtg aggcagtaaa gaaaatgact gctgtgttat tgatttccga atatttttgt
    44341 tcgatattac ttttgacgta aagagccccg ataacggcat cgccggtcgg ggacaggatc
    44401 ggttgaacat tgatattcac ccgtttatcg tcgttatcta aagcaacata acgtttagtc
    44461 gtgaaatcat tgatatcaac ataatcattt ttctttccca cagcgctttg ttcattgaca
    44521 tcggttgttc ctcggatgat ccctttttcg tcaacgaccc gtatttcgat gatatcgcta
    44581 gtcgcactgt tttcaataaa tcttttgata ttcgcattta cctcttcatc atctgcatta
    44641 tcttttcgat tcaattccgg gctgattgtt gttgctaatg attccaccgt ttgattcata
    44701 tctttaataa atgtattgat cgtggaacgt tctaaaccac gtataaagta ggcaccaata
    44761 atttcgatcg agatcaataa aatcagaata aaggtaatag cgatcttaaa attgactgat
    44821 cgaaaaaagc ggacttttct tttcatccaa ttactcctgt tctgggttac gcaaatagta
    44881 accgacacca cgacgtgtca ctaaataatt tggatggctt ggattatctt cgatcttttc
    44941 acgcaaacga cgtacagtta catccactgt tcgtacatct ccaaaataat cataacccca
    45001 aaccgtttgt aataaatgtt ctcttgtcat cacttgtcca aggtgcttcg ccaagtaaaa
    45061 gagtagttcg aattcacgat gtgttaactc gattgtttct cctcgtttcg tcaccatgta
    45121 agcttctgga tggatcgtca aatcaccaat cgtcaattcc gcaggtgctg cctcttctgg
    45181 ttccttcgca gccgtggcac cgcgacgaag atttgctttg acacgtgcaa ctaattctcg
    45241 gttggaaaat ggcttggtca cgtaatcatc ggcgcctaac tctaagccta ggactttatc
    45301 aatttcagaa tctttggctg ttaccataat gatcggcata tcataagttt tgcgtacctc
    45361 acgagcaact tctaagccgt ccattttagg tagcatcaaa tcaagtaaaa tcaaatcagg
    45421 ttctacctca gcaacttttt ccaaggcttc ttccccatca tatgcagtat aaacgtcata
    45481 gccttctttg gctaaattga atttgacgat atccgaaatc ggcttctcat catcgacaac
    45541 taaaactttt ttcataaaga gccacctctt atatattatt gtcatacctt ctcattatac
    45601 cgtatttctg cttttgtttc atctttgcct ataaaacaaa agagaaaatt aaaatgaaag
    45661 tgcaaaatac tttattattt tttgttctaa cttcaataaa aaaggaaaac actgattttc
    45721 acctcagtgt ttcctttttt tgatttatcc ttctaaaact tgtacaccga cacccaataa
    45781 tcccggacca gtgtggacta ctaatgccgg agaaatctgg ccaaagtaga tttggtttgc
    45841 ttgtgggaac tctcttttaa gtctcgcttc catttcaatt gcttcttctt tcgcatcccc
    45901 atgggcaaca gctaagttga agacctttgc attaccgatt ttttctttga cgtaagcaac
    45961 cgttttatcc aaactttttt tacgtcccct agcttttcct actgtgtaat aaatcccttc
    46021 ttcattacat gagatgattg ggttaagctt taaagcggtt cctacaatag aagcaactaa
    46081 accgatccgt ccaccttttt gaagatattc caatgtcgca acattaaaga aaacttttgt
    46141 tctttcgact aactcattca gattactgat cagttgatcc cattctactc cttcttccag
    46201 ccattttgct gcttggatcg cagaaaaacc tgcaccaata ccaatatttt tcgtatctag
    46261 tacatatgct tccaattctt cttgctgttg tgcaattaaa cgaaccacat tatatgtccc
    46321 gctcaatcca ctggaaattg ttaccgcaag taccttttca taaccgtctt ttttgatttg
    46381 ctcaaagatt tctgtgatcg actcaccatc cggcaaagat gtaccaggga tttcctctgg
    46441 caaacgttga tagatctctt caggggtaat gtcaatttta tctgtatata cctgatcttt
    46501 ataaataatt ttcaatggga tcatatacat ctcgtatttt tttatcaatt cttctggaac
    46561 atccgtacct gaatctacta aaattgcgat tttattgttt ttcatttgtt ttctcctctt
    46621 ttaaatattc ggtttctaga acaatcgttt tttccgcaag aagtttcatt gcaaacgata
    46681 aagttgccgc tcttacagca atatattcta caggggttgc ctggtcgaat ttttgcggat
    46741 gagatttacc ttgtgctaat tggctgacca tccacacagc tgcttcttgt tcatcgcaaa
    46801 atagattata ggcttcacga attcctactg tttttccttg gaacaatatc ccttctttga
    46861 tttcaggaat agtcaatact tgtttcaacg tagtgattgc aagtaaaaat gctacgtgtt
    46921 ctttgttata tcttttttta actggtgctg gtatcaaacc tagtttaacg taattgttta
    46981 ccatagaaga ggtaagcaat ggatgctttt ctccttgtat cacaggtgaa agataacgat
    47041 caactaacgt tctgacttgg tccatataaa gttctaagtc agggagttct tcccagtgag
    47101 gcagacggaa tgtttctagt tccttccccc actccattaa ttgctgacgc attttttcca
    47161 tcataaccgc tccttttttc atttcataaa catgatatca gacttatgat aaacattcaa
    47221 tctagttgct ttaactaggt gatttgatta gcgggattcc atgaaaacaa aaaacaagaa
    47281 ggctaagcct tcttgtcttt tatcgttaag aacaggagac ccgccgggga gacggcggga
    47341 aaggagtaaa aaatgaaaaa agtgttaggg tttgttctct tgcaagaact ttgtttgttg
    47401 gtatgcttat ataatagcga gcaaatgtga ataatctatg aatgatttcc tataaggaag
    47461 taaagatttt tctgattttc tttaaatgta gtcttgcatt ttaaaattct tagtgctact
    47521 attattaagt actttttgta cgggccgtta gctcagttgg tagagcagct gactcttaat
    47581 cagcgggtcg cgggttcgag cccctcacgg cccattgggt gccaaaccca cggaacaggt
    47641 aaatgttggt gcgaaagcat ttcgaaaaca tttacgctgt tcttttttta tttttcaaaa
    47701 taactagggc tttttatttt taactcaaag tccactgtag ccctttggac tttgagttaa
    47761 aaaagaagca taattatgaa cagtaaagtt tctaatataa caataattcg agaagctttg
    47821 caaaatcatg ctgccaatga actctctaaa catataggat taaatctaag tactataaaa
    47881 ggttaaaaag tgaagaatat ttaatagaaa aactaaattt acttgctgct ttcccctttt
    47941 ccattaatac aacaatcaga gagtgtcttt ttttcgaaaa aattagaata aataaaagac
    48001 acgtagtttt tttatctaca tgtcttccat cgactacaag ttatcatttc tcaaaacgta
    48061 tttagctttt aattgattaa tcgtttcacg ttgtctacac attgcctaat taagattttc
    48121 ttattagaag catcaatccc gcaataatac ctaaaactcc aggaattacc cacaataatg
    48181 ttaaaagaaa aaatggaata atagataaaa tgagtaaact aataccatag accactgttt
    48241 tttgattttt tcttactaaa aaacctatta tgaaaatcat tatcgaaaaa actagggtaa
    48301 ttcctccaaa taaaacaaca ttcccgctta gattgactaa catatctata tccttactgg
    48361 ttagttctgt agtatttaaa gtagttgaat caatagaaga tagaaacata ccacccatca
    48421 atgctaaaag actacataaa atccctgtta ttccactaat taaaatcaaa ataaactctg
    48481 tttttctctt attcatttaa ttctctccag attaagtgtt ttagaattag aaattttttg
    48541 accatagtta atagcttcta atgaacgaat aacaaccact gacgtgttag aaaaataatc
    48601 cccaatatgt tgtttcttgg gcgtgaaagc agccaccaaa tacccaattt gaaatacaaa
    48661 attttttaat ataaacctac atatacactc acgaataaag atagtttgcc aacttagctc
    48721 tttttcctca aagctcacta ctcttatccc aaaaatcatt ttcccaattg tttgtcctga
    48781 attcagctta gtcaaaagta caaaataggt taaataaata gctaatgacg ctataccata
    48841 aaaagtaata atggatttgt cagattccag tcccatgatt ttgtaaaaga gacctatagt
    48901 agcatgggtg atgatgctaa tacaaattaa atcaataaga aaagcaaaaa atcgtaacca
    48961 aaatcccgca aatacataat taggcaaagt ttcaatgcca ctcttcttat tcattttata
    49021 gccctccata gtaatacatt ggttttggag tatcagaaaa tccaagtttt tcaaaaactg
    49081 aaattacacg atctgatgta gttgccttta gcccttgcca ttcagcaatc ttattaccta
    49141 accaagaaga agcaaagcca gttgtgtcat ttttatattc tataaccgtc gcatcagtta
    49201 atttcttctc tttttttagg gaatctaatg catcttcaga gtaaccaatt tgatccacca
    49261 acccattttc tactgcttgg ttaccatcat aaattcttcc atccgcaagt tctttcactg
    49321 cattagtatc catatttctt ccttttgcta caacattgac aaaacgatca taagcactca
    49381 tcacaaattc ctgtataact ttattctctt cctctgaagg tttgtgttga ggtgacatca
    49441 tatcttttaa tgcaccactt ttatacgtcg catcagttac gccaagtttt tctaataagc
    49501 cagaataatt taaaccagaa ataatgacac caattgatcc agttgttgtt tcttccgtag
    49561 caaatatttt atctgaacca gcagaaatgt agtaccctcc acttgccgct gtatttttaa
    49621 atgcagtata gataggaata tctagctttt taatttttgc catttcttta gcaatctcag
    49681 cactttcata tattcctccc ccaggtgaat ttacttctaa taacactccc ttaaccgctt
    49741 tgtcttcttg aattttcttt agctgtgtta aaaaattctg atggttatat tgttccctac
    49801 taaaaagatt gctttcacct gtatctgcaa taactccatt gacggatagt ttgacaatct
    49861 ttttatttga tgcgccttct tctaacgttt cttctgtgat ttcgttagat ccgtaaaaaa
    49921 tcttatttat tcctgttagg gttgtatttg atgcttcctc tttatctttt cctggaatta
    49981 gcgcgacgat aatggatagt aacaataatc cgcacgctac tcccacagca atccatcttc
    50041 ttttattcat ataaactcct cctatattct tagtaaaaat aattattttt tatttagaaa
    50101 cacatcgaca atttgttgcc aatatgtttt ttctcctaca attaaagaca actctccagt
    50161 taatccataa cgaaacggaa acgattgagt tgtttttaac gttcctttca ctgcataaaa
    50221 aacacctttc tcagtggcag tagaattttc tgaaatttca tctaaagttc cgtcgatcgt
    50281 aatcggagcc ccaccttttt gatccaattt aaaatgaacc ttcatccctt ctttaatata
    50341 ggtactttca ttcgcaggta aaagtgctgt aaatttcatt tttgattttt cgtggatcgg
    50401 ataaatttct gcaagcactg ttcctttggc tagttctgct tgtccctttg tttcttcatt
    50461 taaatgaacc gttccagata caggagcttt tagtattcct tttgaaagtt gatcgtccaa
    50521 acttttgata actgactggt tcttctcttg tttttcattt agctctgtaa ctgtttgttt
    50581 tacagttgtt attgcttgtt ctagctcttg ctttcctttt tccgtttgac taattctgtc
    50641 attctcagaa gtagttggtt taactaattt agctttttct gactgatact gttctacttc
    50701 tttttttagt tgctcaattt gttcatttat gcttgctgaa attgtcagtt tcacttgatt
    50761 tttttgatca tcagaaacat tatttagttg ttcttgccaa ttctcatagt ttgccatcat
    50821 ttcttttgaa aaatctttta atgattgatt attactccaa gctacctgta cttgttgcca
    50881 ttcttgtaat ttactatctg aactctcaat ttgtttttct agttgtcttt ttgtcttttc
    50941 gtatacttct aattgctttt gatcatttaa ttcactttgc ttcaaagcgt agcggagtga
    51001 ttctttttct gacaacatgc tctttaattg attactgtag ccaaaactgt catccgttga
    51061 aaacaaattt tttcctttac ttatactttc aataaatagc tgagccatct ttatttgttg
    51121 agtaatacgt tggttttctt gttctatctg agccttttca ttctctatta aagaacaatc
    51181 caatctaacc aacacatcgt ccttatgaac aaattgattt tcttttagga agttttccat
    51241 tattttagct tctaatggca cttgtaactt ctctattttt tcggggttca attgagccgt
    51301 tgtccgaata acaacctctt ttttagcaaa ggttaaaaac aatccaataa tacataaaaa
    51361 aagaattaca gggtataaca cccaacggta gaagctatga tgttgtttag tatagatact
    51421 agaatgttcg aaccaattat tattctgcat aaattgtttc cctctcctag atttcccata
    51481 atttttgata gataccatcg gcaaaacgaa gttcatcgtg cgttcctttt tctaccaaat
    51541 gcccttcatg gagaactaaa atttgatcac atgcttttgc tattggtaaa tgatgcgcaa
    51601 taaaaataat cgttttttct tttaaattga ggagataatt cataattgca tgttctaaca
    51661 aggtatctaa tccactagtt gcttcatcta aaattaatat gtccggattt tttaataaag
    51721 cccgtgctaa ggctaatcgt tgtcgttgcc ctcctgaaag attatttcca ccttcttcca
    51781 acaatgtctc gtaacgtaat ggctgttgat taataaattc atttagttgg actatctcac
    51841 aaacttctat tatttgttcg aaacttggtg ggctatctaa accgaatata agattctcaa
    51901 tgattgttcc actaaagaaa aatgactctt gaggcaaata tgtcactcga tcgcgtaata
    51961 tctgaaaagg aatgtctaag tgattaatgt ttccataatg gatcgatcct tctgatgctt
    52021 cataaaaacg aacaaataac tttgcaagag ttgattttcc agaaccacta actccaacta
    52081 tagccgtttt actgtgcgaa ggaattgtac atgaaatttc gttcaacgtt ggggccttca
    52141 tgctatacga gaatgaaacg ttttttacag tgatatcttg ttgaaataat tgttcagaaa
    52201 gcatatattc aaaagactgg ttcgattttt ctgaatcaat cgcgaaaatt tcatttaaac
    52261 gattatttgc aacttgagcc gtttgcattt tcacttgtaa attaataatg ttttgtaaag
    52321 gatctgtcaa aaatactagt aaagcattgt atgtgataag ttgacctaga ctaattctat
    52381 tatccataac aaaataagaa cctagccata aaattaatgc actgctaatt aattgaatca
    52441 aatgtttaat tccttgttga atattatcaa gcgtaaccgt acgaaatgat tttttcatta
    52501 atttaacaaa ttcatggtct atacggttat atactttctc ttcaccgcta tatgctttaa
    52561 ttgtttctat tcctttaaga ctttctatga tactagaatt tacagctgca ccggctgcca
    52621 tttcttccct gttcgatttt tcatagcttt tcacgaaagc gaatattgca actacataaa
    52681 aaggcaaaga gcatagagtg attaaaaaca acgtactatt ctgaatagct aacgtaaccc
    52741 caacaagcaa caccatacca atatctaaaa atattgataa cgtagcactt gctaaagcat
    52801 caataatctt actggcatca agaaaacgag aaataatttc ccctgatttt ctcgtagcaa
    52861 aaaaattcat tggtaaagtt aacacatgtt tgaaatactg aagcataatt gccatactca
    52921 ttcgctgtcc taaaataata agaagataac tacgactata ttcaaacaat acccgaaata
    52981 aatagacagc gatcaagcca atggaaatta tatttaaagt tgaacgtgct tgattaggga
    53041 tgaaaaaatc aagaatacct tgaaaataat atgagcttcc tatgccaaaa aacgtaatga
    53101 aaatcgatgc taaaacgatg tggaatacta atcctttttg tttccaaatt ataggcaaaa
    53161 aagaacttaa tccagctact ttttctttaa taggaatata agtttctcta ggcataggta
    53221 ataaaagaat acctgtccat tctttttgaa acgcttcaat cgtttttcta ttttttccat
    53281 tagcgggatc tgcaatccat agtacgtttc ccttcgtttt ataaattaca acgtagtgca
    53341 tatagctgtt atcaaccaat atatgtgcaa ttaatggtaa gataagatct ctttctttcc
    53401 agatctcact atctgcttga atagccatac aatcaaaaga caatttttca aatgtctttt
    53461 tcaatccaaa tgctgaagta ccctctaaat cagtcccaga taggtcccgt aatttatgaa
    53521 tcgggatatc agttttaaaa tattttaaaa tcatcgaaag gcaagcaaca ccacaatctt
    53581 tttcatcttg ttgctgcaca aaaggaaatg attttatcac ttatctaaca caactccttc
    53641 taaaaaattt ttatagaaaa attctcaata tagctttaat acagtgaaaa tattgtctca
    53701 taattccaca aaaactttga aaacagttct tgaacactaa ctttttcatt cctgaatcaa
    53761 atttattttg gaaattttac tattcaagaa taaacttgat aagtttcagg aagattttta
    53821 ctgttaatca aatacgtatg ttacagtagc accgtaaaaa atattattta aagaagcgtg
    53881 tttaaacatg caaaatgtaa aggaactaag tattgtggaa atgcacaaac tgttggtgga
    53941 ggcaataatg cttggggaaa attggggcaa gttgtaggtg gacttaccac aggggcagta
    54001 ggtggtgcag gtttaggaac agcaatttgc ggaccagctt gcggtgttgt tggagggtta
    54061 tatggagctg tcgctggagg tgctgcagct ggctgggatg ctcgaaaaaa ataaaaaagg
    54121 agaataagta gtatgcatat taaaaacaca aaaacaacat ttatattatc ttcagaagag
    54181 ttgaaaaata ttcaaggtgg gagtgccgtg ggagttttag gcactacttt ttctggggca
    54241 acagcaggag taaaactttg ttcagccgga ggaccttatg caatagctgc ttgtggtgta
    54301 ggtggagctt tattaggtgc tggattcagc atgtggaccg gtgcgtaaac aattaaaaaa
    54361 tatcaaacct caagattggt ttgtgtcgat tacgaacata ggtatgttct ttatcttttc
    54421 aaaagtttgg aatggacaga actttttact aaagcttctt gtgctagttt ttataattat
    54481 attaaatttt tatcttttat taaagtcaga taaaaattct ttggaaatgt aatggtagag
    54541 atatttttac aaaaggactt gcagcgctgt agttggtgct gcaggaggac ctatttcggc
    54601 cgttacagga ggtattggta atgtacttgt cggatgtgca ttaaagcctg ttcctgcaag
    54661 ttaaatttat aaaaacagga aagtaattat ctcaaatcct atcaagtaca atcataggat
    54721 tgcacttgac aggacaaatt ttttaactaa aaaatgaaaa acactttcta aaaattagta
    54781 gaatttgcca aatctttcgc atcttccacc atacatttaa ttttaattcc cctttatggt
    54841 acttaataaa aacaagaaaa gaactattca tttggccctt ttttggttcc agaccataga
    54901 aatattcctc cagtaactaa tattgctgtt atgcacgtaa ctaataacag ctataaaaat
    54961 tggtacaact ttaataggat tcagaaaaat ttgcactagt gaaggaatct attttattag
    55021 aaaaatgcaa tctccctaaa gaaaaaacca ctaaacagtg ctttctttca acttctcctt
    55081 aattttttgt gctttcccta aagtaaagtt tctggccaac tctgaatcaa aatatagtga
    55141 acgtttttta aaatctattt ctttaacgtt aaagatattt actaaacacg ctcgactaat
    55201 tcttgttaac ataggatatt tcttttcaag ctcactaatt cttccataaa attcgtactg
    55261 cccatcttta gtacacaatg ataatctatg tggtaatttt gaagcttcta caaaataaat
    55321 gtcatttata tcaaaagtaa atgtttgaga acctatggaa aaaacaaatg cttgattcgt
    55381 aacctgtttt gttgcatcta tcctttcttg cgctaataac aataactcaa caatctcgtt
    55441 tcgatactca tctagtgttt gatcttttgt tacaaatcct aacgtttcaa ctcttctttt
    55501 tattgttacg ggtaacatct catcatgagt agttgtaaaa atgatttttg cttgaacatc
    55561 atattttctg attacttctg ctaattctat accgttaact tcatgattta aatcaatatc
    55621 taagaaatat attccttgct ttggtcggaa ttgtttgagg tattttttaa cttctaacgg
    55681 actttgtgtt ttcaatacta tcttaaatcc tgaataattc atacttttaa ttaaaaccat
    55741 gtgaaactgc ctcacggcag cttacaatta ctttttcatc ttcacccgtt aagtgatgaa
    55801 aaaagaaccc ctttctgcta tggttaaagt gactaaacca accaaaagaa aggagttctt
    55861 ttcatgacta gcttacacaa aaaccaagta aaattcaatt caaatatcac tatttctcat
    55921 acaggtggtc aattatcgag tgattcgggt ctcgtcctag tgaaagagct gatgaacatc
    55981 tttggttttt ctgaactggc taagcaacac attcacattg aagacaaacg agcgtatttt
    56041 actcatgaca acttatccat acttgagcag ttcattatgc aattaattgc tggttactca
    56101 gctgattctg cagctaatct cctgagacaa gatcctgtgt ttaaagctgt tttagatagg
    56161 aaagaactcg cttctcaatc ttcactttcc cgatttttag atcgactatc tgaggagaat
    56221 atccatgaac ttcaagcttt gaaccaagaa cttatcgata aagcacgcct catccgtaat
    56281 gatacggaat taatcattga tttagattcg acccattcag atacatttgg tcaccaagaa
    56341 caaacgaatt ataatacaca ctaccaaacc tatggttacc atccattggt agcttttgac
    56401 ggattgactg gtgacttctt aaaagctgaa ctacgttcag gaaatcaata tacatcaaaa
    56461 ggcgtaaagg agtttctcac acctttatta gagcactata atcactctct accaaacact
    56521 gacatcttgg ttcgtggaga cagcgggttc gctacacctg gtgtgtatga ttcgtgtgaa
    56581 tcaaaaaaga gtcattatgt tattcgactg aagaataatc gtagactagg tcaaatagct
    56641 gagaaatcag tactttatgg cgataatcaa aagtgggaag aacgagaagt tcagtacttc
    56701 tcgaccactt atcaagcaca atcctggtca caaagtcgtc gcgtgtgtat acgctcaaca
    56761 cgtgaagcgg gcgaactact ctttcgacat gaatttatcg tgacgaatct atcagaaaat
    56821 gtttctcctg ataccatctt ttctatctat gccaaacgtg gcacaatgga gaacttcatt
    56881 aaagaagcga aagctggctt ttactttgac aaaacagaca gtcctcgctt tttggagaat
    56941 catgtccgaa tgatgctcag cttgatagct tacaacttag tcaacttctt aagaacgatt
    57001 ggctttgaag aagtccaaaa gggaatgacc attcattcta ttcgattgaa gtttctgaaa
    57061 gttgctggga aattagtcca aacgggtaga cgagtctatc tcaaattatc tagttatcat
    57121 gtgtatcaga atgaattcta cagggtcttt gctcgcctga ggcgagccag tcaatggatc
    57181 taatccaaca atctaacgat ttttttactg ggaagttatc cagggagaag tatgctcaaa
    57241 atgccttaca cccaaaattt attcctcaat atttttgaac agtgaatgtt tgaagtaact
    57301 ttttagtcaa aatcagttga tagggatatt agtgatacat atttcaataa aatgtgccaa
    57361 agaattaaag ctatgaatta ttcaggttaa atactttatc gtgaaaaaga atgaaattat
    57421 caataattcg ttctaactga tttaattgta taatttgatc ttcacataat attattggat
    57481 aactcataaa tcctccttcc ctcaaaacaa caaaataact tgagtagtaa attgatacgg
    57541 atcgttttga tagttcacaa acatattggg attcttttta ttgatttcct ctatactaga
    57601 taaacctaac ccttgatgtc ccttttttgt tgttattcct tttttcatta gagtatctat
    57661 agagagtagt atctctctac aactattcga tatcgaaatt tctatctgtt tatcatctcg
    57721 gtaaatagct agagacagtt ctctatcttg actctctata gatgcttcaa ttgcattatc
    57781 gagaataatt ccaagcaccc taatacagtc aaatattgga acaggaactt caataatagt
    57841 acttggacat tcgaatttac aacaaatctt attgttattt gcttggtata gcttcgctat
    57901 caacaaactt ttcaaatatt cattttttat attttttaaa taacgatatt caaacttatt
    57961 ggttagataa gaattagaat agatctctaa ttcttttatt tctttttcca atatcgaatc
    58021 atggttttga ttagcgattt cttttaaact cagcaataaa tttttatagt catgtctaaa
    58081 tctggcaaat ttttcttgat ctttttccag tgaatcagta tattttttta gataaaccaa
    58141 ttcttttctt ttcaattgct gttcatattg ttctcgttgc ctaatagtta ttctaataaa
    58201 taaaaataga acaacaaaca tttgaatgat taaaaatatt aagacaccaa acacaaaatg
    58261 atcaaatact tgatattgat gagcaacata agaaacaaat aaaccaattg taagtaaata
    58321 aatcatgaat atagcagtaa gtttagaact ggactgttca acaatcctat ttatagctaa
    58381 ttttctatat aaccaaataa acatcagcaa aaacaaagaa tcaagcatta tctggaatac
    58441 tacataacca taccctttta tcccttcaaa tgaaaacata tacgtcataa ccatggaagt
    58501 aataatagat ctgaaatcat caataagcat acataatagt aatttattta aaagcactat
    58561 attagttttc ttcttttcct ttaatataaa ataacttccg attataggta tagctgcgga
    58621 aacatcagta aataattcaa caaaaaaagt gagtggtatc aataaacata caaaaagata
    58681 ttttcttttg ttaagatcag tattagtcat ataaaataag agaaaaaaga aaaagtttcc
    58741 cacgaacatt cttaatagat aagcttttgt atcgatagta atcaagagat cccctcctga
    58801 ttttaggata tcataaaaaa aattttagtt acacttttta aataatgaaa aaacacattc
    58861 cactaaattt gatgccggtt ttccttgagg ttttgttcca gcacctcctt taattttttt
    58921 aagctcttga tctgaacatt gtttagcatt tagtctattt ttttcttcca ttacgattcc
    58981 ccctacaaac tataaatact tttatgtcac tgattcaata tacctatttt tctaatcaaa
    59041 aaattttgag acatatttaa tattataagt atcattaaaa ttgagattta tctccataat
    59101 ctgctcgata taaaccattt attccagcag ttcttccaat ttcactcaaa tttcttatta
    59161 attcctcttg atattcagtg aatcttattt tattagtata agcagtaaaa tatagataat
    59221 tcaccaatct atttattaaa ggaccgggat ctaattcata tttttgtttt tctaatttta
    59281 aatatacctt ttgtaaaacc tcaagaatat cagaatattt aggatcttta cttatagata
    59341 tatcattata taattcatga acaatttgct tagcattttt tttcatttta gcacttccct
    59401 ggaattgctc cacctaaaaa accacctata gacattcctg caatacaagt agttgccttg
    59461 gcccaatcga ccgtacattt atttttagtg caatacactc catttccata atattttcca
    59521 ctatgagtgg tacccccata gataagttgt gtctctttaa tagacaaaat ttttaaatgt
    59581 ttcataatct aatcacccct aaattaatgt ttttagacta ttattctaac attcattaaa
    59641 tttcaaaaaa caattccttc ttgaaacgta gcaaaataat tcctgaataa gaatttaatc
    59701 acttttaagt atataaacta tgtatattct ttaatatttt ttgagaatgc acttcgtaca
    59761 taataaactt taatcacatt agtctttcat acctacgatc gatctctttg actcgcaccc
    59821 ataaattcat aatagtttgt cggcgtcttt ggacgtttcg aagatgagct acgcattctc
    59881 tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg gatatttgat
    59941 ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca gctttcgact
    60001 aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt tccattgttg
    60061 atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc taatactttg
    60121 taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat ctgctcttcg
    60181 cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc aaacatccaa
    60241 tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg acctagtgca
    60301 acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt gtcatatttt
    60361 tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc tatgattttc
    60421 ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc tggcactgct
    60481 aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc tttatattgt
    60541 gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc tgactcgcca
    60601 tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac atcatagccc
    60661 atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc ccattcaaca
    60721 taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat accaatcgca
    60781 cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt acttttctgt
    60841 gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt aaaacggtca
    60901 atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc gacttgtgct
    60961 ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat agaggcaata
    61021 acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat cgtactttta
    61081 gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa
    61141 gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg aacggttggt
    61201 ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc gaaaaatgca
    61261 actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag gatcggtttg
    61321 ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac cccaatgatg
    61381 atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc attccataga
    61441 ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc
    61501 ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc tggaatcact
    61561 aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat
    61621 aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt tgccactggt
    61681 aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg ctaatgcttc
    61741 aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa ctgcaagtgg
    61801 agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt tcccaatcac
    61861 cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag cttctaacgc
    61921 ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca tcagcatgct
    61981 gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt
    62041 cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat cgacttcttc
    62101 cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac ctgttccgat
    62161 ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta aagcttcatt
    62221 caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt ccagtaattc
    62281 gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt ccatcagcaa
    62341 gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca tcccaccaga
    62401 taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta atgctttttc
    62461 tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt attctgtatt
    62521 ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat tgattttatg
    62581 acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc catcaatata
    62641 gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg tggatttccc
    62701 actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa aattcacgtc
    62761 atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct ggtgatcatt
    62821 catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat gttttacttc
    62881 tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc tgaaaaaagt
    62941 gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc
    63001 caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa gttaatattt
    63061 ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat
    63121 acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg taaatttagc
    63181 ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa ctgtggcgcc
    63241 agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg ctttttctga
    63301 taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg tttgattgac
    63361 cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct ccacacttgc
    63421 catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca ataggaaatc
    63481 ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt ccatgattcc
    63541 aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat tgtacgtact
    63601 ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt caacgatacg
    63661 ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt tctttccaga
    63721 cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct agcaaaataa
    63781 gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct gttccttttt
    63841 tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac gagtatagac
    63901 aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca tcctattcct
    63961 ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga cggaacaaaa
    64021 cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct acctgtccac
    64081 cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct aaaccgcatt
    64141 gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg atagccccaa
    64201 ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa gctgctgcgg
    64261 gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat acttccgcat
    64321 gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat cctgtaatcg
    64381 ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact gccaggattc
    64441 gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa aaattataag
    64501 aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct tttttaacaa
    64561 aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga aaatgatatg
    64621 cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt tttttcaagc
    64681 tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga gctacccaag
    64741 attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt gcagaacaag
    64801 gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca atcgtgcgac
    64861 tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg aaggattttc
    64921 ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc gccatatatc
    64981 cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt agaaatccta
    65041 acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc catcccgcta
    65101 cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca aacaatgtgt
    65161 cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa tagatcagcc
    65221 acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc tgatcgattt
    65281 tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta catagttggg
    65341 taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc aatagtttct
    65401 ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc tcgtaatcct
    65461 ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc ttgccgcttt
    65521 gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga tcatatgtac
    65581 agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg attagttttt
    65641 cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga ccataggaaa
    65701 gcttttctag attgattttt tttccttctt ttttgatgac atccatcata ttcagaccaa
    65761 tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt cgttctaaat
    65821 tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa taatctcttg
    65881 caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg tcctgaatat
    65941 gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg cccggccaat
    66001 tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc ccgctgtttg
    66061 gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga ttagttcggc
    66121 ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg gatctcccag
    66181 aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat ccattaaccg
    66241 tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc ctgcttttaa
    66301 ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa agatttgacc
    66361 tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg cattgttgcc
    66421 tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt gcaaatggcg
    66481 ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct gctttgcctg
    66541 cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta aaatcattca
    66601 ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc tcacatagaa
    66661 tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa tctttagtat
    66721 tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt tttctctatt
    66781 aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa acacaaaact
    66841 tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact tttttactaa
    66901 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
    66961 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
    67021 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
    67081 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
    67141 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
    67201 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
    67261 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
    67321 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
    67381 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
    67441 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttct atctattatg
    67501 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
    67561 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
    67621 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
    67681 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
    67741 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
    67801 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
    67861 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
    67921 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
    67981 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
    68041 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
    68101 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
    68161 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
    68221 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
    68281 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
    68341 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
    68401 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
    68461 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
    68521 aacggcaaac aggaatatga agtattagga acggatatct catgtaactt aggctcgaca
    68581 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
    68641 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
    68701 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
    68761 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
    68821 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
    68881 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
    68941 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
    69001 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
    69061 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
    69121 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
    69181 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
    69241 atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac acgtcgatca aggcatgagt
    69301 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
    69361 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
    69421 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
    69481 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
    69541 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
    69601 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
    69661 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
    69721 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
    69781 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
    69841 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
    69901 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
    69961 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
    70021 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
    70081 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
    70141 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
    70201 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
    70261 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
    70321 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
    70381 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
    70441 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
    70501 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
    70561 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
    70621 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
    70681 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
    70741 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
    70801 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
    70861 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
    70921 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
    70981 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
    71041 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
    71101 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
    71161 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
    71221 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
    71281 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
    71341 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
    71401 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
    71461 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
    71521 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
    71581 ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa gcattttcaa tattggacta
    71641 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
    71701 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
    71761 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
    71821 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
    71881 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
    71941 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
    72001 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
    72061 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
    72121 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
    72181 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
    72241 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
    72301 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
    72361 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
    72421 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
    72481 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
    72541 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
    72601 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
    72661 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
    72721 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
    72781 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
    72841 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
    72901 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
    72961 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
    73021 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
    73081 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
    73141 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
    73201 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
    73261 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
    73321 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
    73381 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
    73441 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
    73501 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
    73561 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
    73621 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
    73681 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
    73741 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
    73801 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
    73861 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
    73921 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
    73981 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
    74041 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
    74101 tggtgagatc ttaccagatg acggacagat caattggcaa aaaaatattc acaaaggtta
    74161 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
    74221 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
    74281 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
    74341 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
    74401 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
    74461 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
    74521 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
    74581 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
    74641 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
    74701 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
    74761 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
    74821 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
    74881 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
    74941 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
    75001 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
    75061 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
    75121 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
    75181 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
    75241 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
    75301 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
    75361 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
    75421 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
    75481 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
    75541 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
    75601 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
    75661 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
    75721 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
    75781 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
    75841 aacataaata cagtattatt tcggttgctt gctgcttcac ctagcccttg tactcgtgtt
    75901 tgattggcga cttgaccaaa ttgattcccg acgtctaata aaataatagc taaaagcaaa
    75961 atggccaaat ggcttccgcc aaccaataaa gcaataaagc ttattgtttg cataacaagt
    76021 cccatataca cgatttttct ttcagaataa gaatctgcca gtcgtcccac gataggtgca
    76081 gcaactgctc cagacaaacc aaaaatcgct aatatccctg cttcgaatgt tccccaattg
    76141 taggcgggac tgctgatata aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac
    76201 atgaaaaatc cactgactgc cgcttctctt aatagacgtt gagaagcaac taaatgcggc
    76261 aaacttttaa gtgaatcaag gtatgaaaca gctgtactac tttgctggac agaacgcggc
    76321 attttccagt aaagcagaag tgtaagaatt ccgactgcac ataaagcaaa taaatagatc
    76381 atccgccaag aaaaaagact ggcaacaata ccagataccg tccgagaaag caaaatcccc
    76441 acgagtaaac cgctaagcaa tgtacccatg accttccctc ttgcttttgg tcccgctagt
    76501 accgcaccgt aaggaatgat gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa
    76561 gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat
    76621 aacgctgcca ttcgaatgat caattttgga cgatccacca cgtcccctaa aggaactaaa
    76681 aacagtaacc ctaacgcata gcctaattgg gtcaacatcg tcaatatccc gactgcactt
    76741 gtgctaacag ataaactagc tgctactttt gttccaatcg gctggatgta atacatattg
    76801 gcaacaacaa cgccacaagt gattgataat agtaaaataa gcgaagttgt taaaggtttt
    76861 ttttcattac tcttcattga tttcccccat ttcctatctt agtttacaac ttatggaaaa
    76921 gattggaaat agaaaaatac aactattcgc cacacttatt atttgtgctt ttgttttcct
    76981 tgttttttct tgcgatcttt ataaatgaag acaactaacc ctactagcac agtaacactt
    77041 gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca gagcttctcc accacacgca
    77101 tacaacacag acgaagggat gactccaatc acgatcgaca atatgatttg ctgtagaggc
    77161 aatttcatcg catctgcagc aaaattgacc atagaagatg gaataaccgg tatcatatag
    77221 cctaacatta cacctatttt tggatgtttc atttgtcgga ttgcttgcac ccaatgattc
    77281 gtttcggttt tcttatctaa aaattttaag cgatgcataa ggaaaattgc agtgatgtta
    77341 ccaagagcat tacccaaaat gttgatgatc gcacctgtca gtggtccaaa gctcaaacca
    77401 gcaatgactc caactactga agtaggaata cctggaacag cacacatcac cgctgtcaat
    77461 acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc
    77521 ttactcgctc tcggatttaa aaataattgg atgtctgtct gatattctaa atacaaccga
    77581 tagcccacta ccagtatcag aatgattccc aacgagatca atatcaatcg gattattttc
    77641 tttttcatat ttcctcctga aaaaaatcta tattcattaa ataatagttt aacataaaaa
    77701 caaaaaagca ccgaaatcca acataggatc tcagtgcttt taaatagtaa aaattattta
    77761 acagttacag atgcgccaac ttcttcaagt ttagctttca attcttcagc ttcttcttta
    77821 gatacgcctt ctttaactgg tgcaggagcg ccatcaacta cagctttagc ttctttcaag
    77881 cctaaaccag ttgcttcacg aactgctttg ataactttaa ctttttggtc tccagctgca
    77941 gttaattcta cagtgaattc tgtttgttct tcagcagcag cagcgccacc agcagcagct
    78001 acagctacag gagcagcagc agatacgcca aattcttctt caatagcttt aactaggtcg
    78061 ttaagttcta ggatagttgc ttcttttaac tcagcaacga tgttttcaat gttcaatgcc
    78121 attataattt cctccgttta attgttcttt tttatttaat gctttatgcg gcaattaagc
    78181 tgcatctcca tcttctttgt ctgccactgc tttgacagcg taagccacgt tgcggactgg
    78241 cgcttgtagt acagatagca acatagatag aagtccttcg cgatttggca attttgccaa
    78301 tgctgtgatt tcttccaatg aagagacttt accttcgatg ataccgcctt tgatttctaa
    78361 tgcttttgct tctttagcaa attcgtccat gattttcgct ggtgcgacta catcttcgtt
    78421 actgaatgca acagctgtag gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt
    78481 tttagctgca cgtgaaagaa tagaattttt gataactttc atttctacgt tagcatcacg
    78541 aagttgttta cgtaaacgtg ttacttcatc aactgttaat ccgcggtagt caacgactac
    78601 tactgatgca gctgctgtaa atttttcgct tacttcatca acgattgctg ctttttttgc
    78661 gataattgct tcactcattt ttgagtttca cctcctgaat tttgatggaa gagttttttg
    78721 cactgaaaag gcaagtcact tttatagtta ctttgctcaa ccagtattat tcatcaccta
    78781 ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc
    78841 tcaaccagta ttgttcatca tctcttcact tcgttcatag tgattcacaa taaaaaactc
    78901 tatgccaccg cagacataga gggactattg aatatattca ataaacgtcc tcggtaggaa
    78961 attaaggctt gatgccacct actgtcttcg gtacaagtta attattaacc tcaactagct
    79021 tagcatgcta aactagttga gtcaagaatt atttttcagt tggatcaatc aacattctaa
    79081 tatttaatta gaaagaagct tggtcaacat ggataccagg tccaaatgtt gttgtgatcg
    79141 ttacattttt catgtattga cctttagctg ctgatggttt agcttttagc aatacatcat
    79201 ggattgctgc aaagttttca actaattttt catcgtcaaa tgaaactttt ccgattggta
    79261 catggatatt tccagctttg tccacacggt aagttacttt accagctttc acttcgttta
    79321 ctgcttttgt tacgtccatt gtaactgtac cagttttagg gtttggcatt aagccttttg
    79381 gtcctaatac tcgtcctaga cgacctacag tagccatcat atcaggtgtt gcaacaacta
    79441 catcaaagtc aaaccatccg ttttggattt tttgaaccat gtcgtcgtca cctacgaaat
    79501 ctgcaccagc tgcttcagct tcttttgctt tgtctccttt agcaaaaact aaaacagttt
    79561 gtgttttacc agttccgttt ggtaatacaa cagcaccacg gatttgttgg tctgcttttt
    79621 tagggtctac gttcaaacgg tatgcaactt caactgttgc atcaaatttt gcaatgtttg
    79681 tttcttttgc taaagctact gcttcggcaa caggataaac tttacttgaa tcaacttttt
    79741 ttaatgcatc ttgcattttt ttgctctttt tagccatttt ggttcctcct tgattgtggt
    79801 tataacggtt gtacctccca cgtgttctat atctttcaat atagagccaa ctgagaagct
    79861 gctgtcttag ggtgtataga ttattctaca gtgatcccca tgcttcgtgc agttccttca
    79921 accatgcgca ttgctgcttc aacgttagct gcgtttaggt cttccatttt taattcagcg
    79981 atttctctta cttgatcgct agaaactttc gcaactttat ttttgtttgg ttcgcctgaa
    80041 cctttttcga ttttagctgc ttttttcaat agaacagcag ctggcggtgt ttttgtaacg
    80101 aatgtgaatg aacggtcttc gtatacagaa ataacaaccg gaatgatcaa acctgcttga
    80161 tcagctgtac gagcgttgaa ttcttttgtg aatcccatga tattgatacc cgcttgacct
    80221 agtgcaggac ctactggtgg agctggagtt gctttacctg caggaatttg caatttaacg
    80281 attttttcta ctttctttgc cacgagacat acctccttaa gtccgtgatg tggttaattg
    80341 gagatgcata tttctcctcc cacgttttat ctgatctgaa aaaatccaga caaataatgt
    80401 gcaaaacaat gcacactggt acagtatagc aaatttattt gttttttcaa gatttttttc
    80461 tgctattttt acattttgct tgaataaacg ctaaatttat tctaaaaaaa caacaatttt
    80521 tcgttctcat ttcttctttt atgttacact tgcgctagca tggttcaaca gatgaaagca
    80581 aacaataaca cggtttttta gacccttgta caaagattcc tacataacaa aatgaggtgg
    80641 agaaaatgaa agatttaagg tatagaagtg tctttgatat tattggacct gtgatgattg
    80701 gtccaagtag ctctcacact gccggagctg ctagaatcgg gaaaatcata cgtagtatat
    80761 ttggcgaaca gcctgattct gtagatattt atctatatga atcatttgca aaaacctatc
    80821 gggggcacgg aacagatatt gcattagttg gcggcctgtt agggatggaa cccgatgatg
    80881 aacgtttagc tgattctttg aaattggcat atgaagccgg tatggaagtc ttgtttgttc
    80941 ctaagagtga aaaagccgaa catccaaact ctgtaaagat gcttctaaaa aaaggaacac
    81001 gaaagctttc agtcactggc atttcgatcg gcggtggcaa tatccagatt tctgaattga
    81061 atggattcaa aatttcttta agcatgggta ctcctacctt gatcgttgtc caccaagatg
    81121 ttccaggtat gattgctaaa gtgactagta tactttctga gaccaatacg aatatcggca
    81181 ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat tatgataatt gaagtagatg
    81241 atcctcaagt agaagaaacg gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa
    81301 attatttcga ttaaggagct aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag
    81361 caaaagctta tccaagtgta gcagaattga tgattgcagt tgaaatggaa atgagtggcc
    81421 gttcccgtag tcacatcatc gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa
    81481 tcaacgaggg aaaagaaggt gttacctccg tcacaggaat taccggtggt gatgcaaaaa
    81541 aattagatga ttatttaaat gaggcagact ttttaagtgg agaaacaatc ttaaaagctg
    81601 tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat ggggttgatt tgtgctacac
    81661 cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat ggctgtccga gatcggcttg
    81721 atttgacaca tgaacagcaa ttggaattct tatttaccgc tggcgctttc gggttagtca
    81781 tcgcaaacaa cgcttctatc agtggtgcag aagggggatg tcaagcagaa gttggttctg
    81841 caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa tgggactcct catcaagcag
    81901 cacaagcagt agccattaca ttgaaaaata tgatgggact gatttgcgat cccgttgcag
    81961 gcttagttga agtcccgtgt gtcaaacgta atgctttagg ctcttctcaa gcttttatct
    82021 ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg
    82081 caatgtacca agtcggacgt caaatgccgt cgattttcaa agaaacagca gaaggtggct
    82141 tagctgttac gccaactgct aaacgacttg cggaagagat tttaaataag agggcgtgat
    82201 aaagctcctt aatttcgagt attaagagat tagttatctt cataacaaaa aacgaagaga
    82261 ggactaattg cccttctctt cgttttttat taatccatca atttcgtatt ttttacttca
    82321 tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt caggtgttca
    82381 aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac tggtcggcca
    82441 atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc tcgacggaca
    82501 ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt tggcaatact
    82561 tcaaaggacg caggacctcc atctagttga cgtccaccat gattggatac ccagatacca
    82621 tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc ttttacgata
    82681 actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt
    82741 gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc actttgttcg
    82801 ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat gacgtcttct
    82861 tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc ccctgcttct
    82921 actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata taattggaaa
    82981 aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg
    83041 ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc ttcccctttt
    83101 tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc taattcaatt
    83161 ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg tggcatgatt
    83221 ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc agaaccaccg
    83281 cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc tagttaggca
    83341 atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat agtagatcct
    83401 tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta agcaaacatt
    83461 tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa gctgatctac
    83521 ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta ctttttcctc
    83581 tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt ttaacggtac
    83641 tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa atcgagtggt
    83701 tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa cgacagcagg
    83761 cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat cattaaaagg
    83821 cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc catacgttcc
    83881 taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga ctttatctat
    83941 gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt gattccactc
    84001 gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt ttccagacaa
    84061 atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt cttttactgc
    84121 ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg tcccgctgta
    84181 gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg attctgccgt
    84241 tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac cacttaacgc
    84301 aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat ttttgattgg
    84361 ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa ggccaatcat
    84421 atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg aaatcaagtt
    84481 ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct ttttctccgt
    84541 caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt ttcacgactt
    84601 cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa cgcaagatat
    84661 gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga
    84721 atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc atgatcattt
    84781 caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc ccatttttga
    84841 cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc attttcatac
    84901 tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa tatgtatgca
    84961 agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat tgtaaaataa
    85021 aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa aagtacgttg
    85081 tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt atccattacg
    85141 taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc tttacggagt
    85201 tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt tagaaatttc
    85261 atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta tattgattac
    85321 aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca ctgactgatt
    85381 ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc tttgccattt
    85441 acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac gtaacttttt
    85501 acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga tatgattaat
    85561 ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa aacacttgta
    85621 tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga agcattaaaa
    85681 attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg taatattttc
    85741 cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg tgatatgacg
    85801 cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga tacttctttc
    85861 ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga cagtgaaaat
    85921 catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca atcttggagc
    85981 gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg agatttggct
    86041 gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac tcttgatatc
    86101 gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac tgaagcgaaa
    86161 gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt agaaattgaa
    86221 aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt
    86281 cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga tctaaataat
    86341 acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc gtgccatagg
    86401 ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt cccatgctga
    86461 tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact ttgttttgca
    86521 ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc acatccatga
    86581 taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc caaagcaatt
    86641 tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg atcggccctg
    86701 aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg atgccattta
    86761 caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct cctcttttct
    86821 cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca aacaataatt
    86881 ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag caaaagctta
    86941 aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg taaggggtca
    87001 taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg ttgtgaaact
    87061 taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa cggtctagca
    87121 agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac gtaagtaatg
    87181 gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt tcattttcct
    87241 agacttctgc tataataacc aagaattaat tttgtaacag atttttttga aaaaacaagt
    87301 ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc aaacaggagt
    87361 attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata
    87421 ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg
    87481 gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat
    87541 gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag
    87601 aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag
    87661 gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt
    87721 tctggagatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag
    87781 ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata
    87841 agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc
    87901 catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt
    87961 tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta
    88021 gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat
    88081 cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt
    88141 gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt
    88201 atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt
    88261 atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct
    88321 tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt
    88381 cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc
    88441 acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt
    88501 ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca
    88561 caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc
    88621 aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt
    88681 tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa
    88741 atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa
    88801 aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata
    88861 tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc
    88921 agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc
    88981 gcactctggt ttcgtttact gatctctttg cgattaaatg ctagattgat gacgctgaaa
    89041 ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac
    89101 agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt
    89161 gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc
    89221 agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg
    89281 cgactctgta tcttgatcgt cggcatcttt cctacttcga cttgttcggt aaagtctgca
    89341 ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt
    89401 gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg
    89461 actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt
    89521 tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc
    89581 agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg
    89641 cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca
    89701 tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc
    89761 gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata
    89821 cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat
    89881 caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc
    89941 taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc
    90001 accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa
    90061 aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat
    90121 caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac
    90181 cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt
    90241 gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt
    90301 catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat
    90361 acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc
    90421 cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt
    90481 tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt
    90541 gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt
    90601 gattacaatt ccagtattaa tcgtttcgag attgccacct tgatcaatct cgccaaaggc
    90661 cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt
    90721 atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc
    90781 tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt
    90841 tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca
    90901 atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat
    90961 cagaagctat tgttcgctga caacacgttt tctgggagta atgggatgtc gatcgtcaaa
    91021 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    91081 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    91141 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    91201 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    91261 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    91321 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    91381 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    91441 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    91501 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    91561 agcatacagt tgatggcagt cgatttccca ctgccgtttg gtcctaacag acctaagact
    91621 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    91681 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    91741 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    91801 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    91861 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    91921 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    91981 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    92041 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    92101 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    92161 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    92221 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    92281 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    92341 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    92401 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    92461 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    92521 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    92581 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    92641 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    92701 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    92761 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    92821 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    92881 accaagggag ggacaccaat gataacagta gtcattattg agatgatcca tttgtgacga
    92941 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    93001 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    93061 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    93121 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    93181 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    93241 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    93301 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    93361 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    93421 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    93481 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    93541 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    93601 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    93661 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    93721 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    93781 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    93841 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    93901 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    93961 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    94021 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    94081 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    94141 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    94201 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    94261 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    94321 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    94381 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    94441 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    94501 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    94561 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    94621 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    94681 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    94741 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    94801 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    94861 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    94921 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    94981 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    95041 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    95101 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    95161 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    95221 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    95281 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    95341 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    95401 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    95461 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    95521 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    95581 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    95641 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    95701 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    95761 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    95821 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    95881 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    95941 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    96001 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    96061 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    96121 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    96181 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    96241 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    96301 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    96361 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    96421 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    96481 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    96541 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    96601 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    96661 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    96721 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    96781 cgaataaaag tgggccatct ttatgttggg caaccgcatc tgctacaaca gtttcgatac
    96841 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    96901 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    96961 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    97021 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    97081 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    97141 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    97201 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    97261 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    97321 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    97381 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    97441 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    97501 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    97561 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    97621 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    97681 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    97741 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    97801 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    97861 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    97921 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    97981 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    98041 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    98101 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    98161 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    98221 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    98281 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    98341 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    98401 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    98461 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    98521 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    98581 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    98641 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    98701 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    98761 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    98821 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    98881 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    98941 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    99001 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    99061 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    99121 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    99181 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    99241 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    99301 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    99361 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    99421 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    99481 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    99541 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    99601 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    99661 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    99721 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    99781 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    99841 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    99901 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    99961 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
   100021 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
   100081 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
   100141 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
   100201 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
   100261 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
   100321 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
   100381 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
   100441 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
   100501 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
   100561 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
   100621 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
   100681 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
   100741 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
   100801 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
   100861 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
   100921 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
   100981 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
   101041 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
   101101 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
   101161 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
   101221 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
   101281 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
   101341 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
   101401 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
   101461 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
   101521 aatccatgct ttgcaaaaag catcaaacca gatgtatccc gatctagtcg cgtcaccaca
   101581 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
   101641 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
   101701 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
   101761 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
   101821 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
   101881 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
   101941 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
   102001 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
   102061 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
   102121 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
   102181 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
   102241 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
   102301 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
   102361 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
   102421 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
   102481 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
   102541 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
   102601 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
   102661 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
   102721 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
   102781 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
   102841 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
   102901 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
   102961 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
   103021 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
   103081 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
   103141 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
   103201 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
   103261 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
   103321 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
   103381 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
   103441 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
   103501 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
   103561 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
   103621 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
   103681 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
   103741 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
   103801 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
   103861 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
   103921 taatccaaac cggaaagctt acaacaaaac gggctgaatt ccctattgaa gaaggacttt
   103981 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
   104041 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
   104101 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
   104161 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
   104221 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
   104281 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
   104341 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
   104401 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
   104461 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
   104521 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
   104581 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
   104641 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
   104701 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
   104761 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
   104821 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
   104881 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
   104941 attaaaaaaa tacgtcgact cccttacaag agagccgacg tatttttttc gtttctattc
   105001 gagaaaatca aaaatagaaa gccttataaa cgatcgatca attcttctaa ttcatttaga
   105061 cgttgttcaa acatcgacat tgcatcttca atatatgcag cctgtgtcat atcaactcct
   105121 gcttttttca ttacttccac aggataatcg ctgttacctg cttttaggta agccaagtag
   105181 ttctctagtg cctctggttc ctggtttagt attttcttcg ccagtgctga agcagcagaa
   105241 aagccagtcg agtattggaa aacatagtaa ttataataaa aatgcggtat ccgtgaccat
   105301 tcgaatttaa tttctgggtc ttcttcgact gctggaccat aatattttgc attcaattta
   105361 ccataactat cacttaggta ttcactagtc aatggcacac ctttttcatc ttccgtatgc
   105421 atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc
   105481 aagtaatggt tgagtacata agcccgtaca cgagggtctt tttctgtttc caataaatac
   105541 tccgttagga tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg
   105601 ccgtacacat aaggctggtt cgaacgagtg aaataactat gaacactatg tcccatttcg
   105661 tggacaagcg taaatagctg atccagcgta tcatgccaat tcaataaaat atatggattt
   105721 gtgtcatagc ttcccgaaga ataagcaccg cttcgtttcc ctttattttc gacaacatcg
   105781 atccaacgtt cagagaatgc tttttctacg atggccatgt attcttcacc cattggtttc
   105841 agtgcttcta aagctttttc ttttgcttct tcgtacgtaa aggtaattgg agcttcccct
   105901 aagaccggtg tataaagatc atacatgtgc agtttttcca cttctaataa acgtttccgt
   105961 aattccatgt atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac caaagtatcg
   106021 tatacacttt caggaatatg attattgctc aaagacgctt ctctggcaga gctgtaatta
   106081 cggactttcg ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta
   106141 tttctaaatt gttcgtaaac actgtacaat cccttaaatg ctgcttcgcg cacccttcga
   106201 tccgtgcttt ctagcaactg accatacacg ccatgagata attggactat ttcaccattt
   106261 tctccttcaa tcgttggaaa aactagatct gcattattca aaacagcaaa tgtatcactt
   106321 gaagcatcaa agatttcacc tgctccagca agaagagatt cctgctcagc agataagaca
   106381 tgggctcgat tatctactat ttgctggata taatggcgat agacttccaa ttttggttct
   106441 tctttgaaat attgccaaat ctggtcatct gacaattgca atatttctgg ttcaaaccag
   106501 gaaacggctt cactaacttt cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa
   106561 tctgtatttc cagtgtcttg atcgttttta aggtgcgcat atacataaat gacttcagtt
   106621 tggcgataaa ccctcaatac gaattcaatc gcatttaaaa attgagaagc gccttgatca
   106681 agtgttcctt tgtgtttttc agattgtttt aactcttctg ataattccaa atatttctca
   106741 tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat
   106801 tcttctcgtt ttggcaactg ctttacttcc atgaaatcca ctccttttct tcccttagct
   106861 ctatctatca aaatagatca tagtctaaca tccacatgtc acagcatcta acttttctac
   106921 tcattcatgt gtctgcttcc atcaatttta gcataactaa atcttgtttt acatagaaaa
   106981 ccgattccta gcctattttt atacttacta ttttttcaaa gaagacaagt gtatgcattc
   107041 gttaaaaaac tgctgataga ccatctcttc atcaatcata ggaaaaagcc attccctttt
   107101 atgttctttt atttggcaaa gccatgagtt gaaatcagga actttctggt tttccaaggc
   107161 ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact
   107221 tttttgatag atccacgatg atagatgcaa aagatgttta tgacgcaagt agcattgttc
   107281 ttgtatggat actgtattct tttgcttgtg aataagttgg cgtgtcagcc atttcttccc
   107341 atcttcactc actttttcta tttttttcat ttggttcatt ttaatttcgt tactagtgaa
   107401 tatttcttta aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc
   107461 tgatacagtc tctgtaatat gatgatacaa ataaagcttt tgtttcttga gatcagcttt
   107521 ccagcaatgc actcctcttt ttctattata gtagcagcaa cttttttcta tcaatgagaa
   107581 ttgcgattgt ccaagaaaag aattaccaag tatccaccat acttggtaac tgttattcaa
   107641 ataattttgg gttctttcaa taaatctctg gtgagacagt ctagaacatt ggatttctat
   107701 tgctagattc gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat aggcttccaa
   107761 aaatactggt tctactgact gtcccagcca atccatgaag cattccttta actgtaagtg
   107821 ctcttttgat tctctttcac taaagaccga acagttatct gatttttgat gggcaaaatg
   107881 agcacatttt ttctttccct ttttcaaaat cactcttttt cgacagccac gacaataata
   107941 tttctttcct ttttctgcat gtttagcaaa acagcactta ttatattcat tcattgcaag
   108001 cagcattttt ctccctctga acttatttac gtctaagaat ttatttacac gaaaaaagag
   108061 ggcgtgaaaa agctattctg catcaagtaa taagaacagc taaacaaaaa tagttcccaa
   108121 tatttttcgt ttggttgggc ttgttaccgc agatgcaagc ttttgaacac atttatacga
   108181 aaaaaagact gcgacacaat ttttgtcaca gtctcttact ataatgctga tttatacaaa
   108241 ataaaatttc gttcgctcga tagcattacg cgccatcaga cattgtccat attcattcaa
   108301 tgtttctggt gtacgatcgc tcttttcagc aaactccaaa attcgagcaa tctgattctc
   108361 tacatccgct tggttgagat tttccatcca gaagtgaacg actaggtaat agcgctcttc
   108421 gaataaatac aaatcagtcc aagcagactc tagttcgaca tctgttgcaa gttggatcac
   108481 ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc
   108541 agataattct tcctccggat cgctgccaat aatctgctga cgaagaaagt cactcacatc
   108601 ttctgaatca acagtagaat cttctgattg agaaatttct tcatccggaa gatttttact
   108661 gataaacagt tctaatccgt ctccttttgg caaaacttgg aaagtgacag cttcgcttcc
   108721 tcggaactcc tcttcgatat caacttcttc caaaatacta taaaagaaat tttcgatttc
   108781 tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat cttcatgacc
   108841 aattagtaca cggatcgtat tttcattaat atgttccatt tccattttgc tacacctcac
   108901 tttgccacaa tgtttaatat agattcattg tagcgaaatc agcttaaatg taaagaaaaa
   108961 aaatctcgct gttaagcgaa tttaataaaa ccagcggatt tttcaacctt tcaagctatc
   109021 ttatttaact ataacatttt tggcagacga atactatata gccgactttt cagctaattt
   109081 tccctctcta tttttcgtgc aatcatcatc ttctactacc tttttataca tctaatcaag
   109141 aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag tcttt
//
LOCUS       DACELF010000002        93454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.002, whole genome shotgun sequence.
ACCESSION   DACELF010000002 DACELF010000000
VERSION     DACELF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.002"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            85..972
                     /locus_tag="IW687_000107"
     CDS             85..972
                     /locus_tag="IW687_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0949405.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IW687_000108"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IW687_000108"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0949406.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IW687_000109"
     CDS             2274..2720
                     /locus_tag="IW687_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0949407.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IW687_000110"
     CDS             2993..4345
                     /locus_tag="IW687_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0949408.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IW687_000111"
     CDS             4516..4803
                     /locus_tag="IW687_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949409.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IW687_000112"
     CDS             5029..5472
                     /locus_tag="IW687_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0949410.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IW687_000113"
     CDS             complement(5478..6662)
                     /locus_tag="IW687_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0949411.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IW687_000114"
     CDS             6930..7628
                     /locus_tag="IW687_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0949412.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IW687_000115"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IW687_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0949413.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IW687_000116"
     CDS             complement(8438..8971)
                     /locus_tag="IW687_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949414.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IW687_000117"
     CDS             complement(9268..11649)
                     /locus_tag="IW687_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0949415.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IW687_000118"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IW687_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0949416.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IW687_000119"
     CDS             12350..12889
                     /locus_tag="IW687_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0949417.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IW687_000120"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IW687_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0949418.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IW687_000121"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW687_000121"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IW687_000122"
     CDS             13996..15162
                     /locus_tag="IW687_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0949419.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IW687_000123"
     CDS             15208..16704
                     /locus_tag="IW687_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0949420.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IW687_000124"
     CDS             16785..17468
                     /locus_tag="IW687_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0949421.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IW687_000125"
     CDS             18113..19303
                     /locus_tag="IW687_000125"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0949422.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IW687_000126"
     CDS             19405..20358
                     /locus_tag="IW687_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0949423.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IW687_000127"
     CDS             20427..21776
                     /locus_tag="IW687_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0949424.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IW687_000128"
     CDS             complement(21959..22282)
                     /locus_tag="IW687_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949425.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IW687_000129"
     CDS             22411..23574
                     /locus_tag="IW687_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949426.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IW687_000130"
     CDS             23644..24993
                     /locus_tag="IW687_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0949427.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IW687_000131"
     CDS             complement(25038..25469)
                     /locus_tag="IW687_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0949428.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IW687_000132"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IW687_000132"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0949429.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IW687_000133"
     CDS             28699..29295
                     /locus_tag="IW687_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0949430.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="IW687_000134"
     CDS             29417..30565
                     /locus_tag="IW687_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0949431.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="IW687_000135"
     CDS             complement(30603..31016)
                     /locus_tag="IW687_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0949432.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="IW687_000136"
     CDS             31095..31526
                     /locus_tag="IW687_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0949433.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="IW687_000137"
     CDS             complement(31625..31858)
                     /locus_tag="IW687_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0949434.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="IW687_000138"
     CDS             32041..33711
                     /locus_tag="IW687_000138"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0949435.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="IW687_000139"
     CDS             33858..34244
                     /locus_tag="IW687_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949436.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="IW687_000140"
     CDS             34231..34929
                     /locus_tag="IW687_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0949437.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="IW687_000141"
     CDS             34934..35398
                     /locus_tag="IW687_000141"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0949438.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="IW687_000142"
     CDS             35395..36306
                     /locus_tag="IW687_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0949439.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="IW687_000143"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="IW687_000143"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0949440.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="IW687_000144"
     CDS             37094..38377
                     /locus_tag="IW687_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0949441.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="IW687_000145"
     CDS             38390..39316
                     /locus_tag="IW687_000145"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0949442.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="IW687_000146"
     CDS             39335..40615
                     /locus_tag="IW687_000146"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0949443.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="IW687_000147"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="IW687_000147"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0949444.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="IW687_000148"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="IW687_000148"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0949445.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="IW687_000149"
     CDS             44940..45716
                     /locus_tag="IW687_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0949446.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="IW687_000150"
     CDS             45713..46645
                     /locus_tag="IW687_000150"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0949447.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="IW687_000151"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="IW687_000151"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0949448.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="IW687_000152"
     CDS             47365..47994
                     /locus_tag="IW687_000152"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0949449.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="IW687_000153"
     CDS             47982..48296
                     /locus_tag="IW687_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949450.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="IW687_000154"
     CDS             48334..48975
                     /locus_tag="IW687_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0949451.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49006..49710
                     /locus_tag="IW687_000155"
     CDS             49006..49710
                     /locus_tag="IW687_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0949452.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="IW687_000156"
     CDS             49707..50609
                     /locus_tag="IW687_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0949453.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /locus_tag="IW687_000157"
     CDS             complement(50767..52473)
                     /locus_tag="IW687_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0949454.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="IW687_000158"
     CDS             52663..53286
                     /locus_tag="IW687_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949455.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="IW687_000159"
     CDS             53736..54785
                     /locus_tag="IW687_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0949456.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="IW687_000160"
     CDS             54782..55663
                     /locus_tag="IW687_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949457.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="IW687_000161"
     CDS             55666..56436
                     /locus_tag="IW687_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0949458.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="IW687_000162"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="IW687_000162"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0949459.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="IW687_000163"
     CDS             57747..58910
                     /locus_tag="IW687_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0949460.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="IW687_000164"
     CDS             59027..59437
                     /locus_tag="IW687_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0949461.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="IW687_000165"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="IW687_000165"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0949462.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61977..62294
                     /locus_tag="IW687_000166"
     CDS             61977..62294
                     /locus_tag="IW687_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949463.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62454..62690
                     /locus_tag="IW687_000167"
     CDS             62454..62690
                     /locus_tag="IW687_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0949464.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63085
                     /locus_tag="IW687_000168"
     CDS             62687..63085
                     /locus_tag="IW687_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0949465.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63752..64696)
                     /locus_tag="IW687_000169"
     CDS             complement(63752..64696)
                     /locus_tag="IW687_000169"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0949466.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64992..65936)
                     /locus_tag="IW687_000170"
     CDS             complement(64992..65936)
                     /locus_tag="IW687_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0949467.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66157..67371
                     /locus_tag="IW687_000171"
     CDS             66157..67371
                     /locus_tag="IW687_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0949468.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67393..69408
                     /locus_tag="IW687_000172"
     CDS             67393..69408
                     /locus_tag="IW687_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0949469.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69491..70864)
                     /locus_tag="IW687_000173"
     CDS             complement(69491..70864)
                     /locus_tag="IW687_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0949470.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70890..71174)
                     /locus_tag="IW687_000174"
     CDS             complement(70890..71174)
                     /locus_tag="IW687_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0949471.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71176..73191)
                     /locus_tag="IW687_000175"
     CDS             complement(71176..73191)
                     /locus_tag="IW687_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0949472.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73370..73675)
                     /locus_tag="IW687_000176"
     CDS             complement(73370..73675)
                     /locus_tag="IW687_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0949473.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73764..74795)
                     /locus_tag="IW687_000177"
     CDS             complement(73764..74795)
                     /locus_tag="IW687_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0949474.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75002..75877
                     /locus_tag="IW687_000178"
     CDS             75002..75877
                     /locus_tag="IW687_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0949475.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76029..76871)
                     /locus_tag="IW687_000179"
     CDS             complement(76029..76871)
                     /locus_tag="IW687_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0949476.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76963..78840)
                     /locus_tag="IW687_000180"
     CDS             complement(76963..78840)
                     /locus_tag="IW687_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0949477.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78837..80258)
                     /locus_tag="IW687_000181"
     CDS             complement(78837..80258)
                     /locus_tag="IW687_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0949478.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80261..83650)
                     /locus_tag="IW687_000182"
     CDS             complement(80261..83650)
                     /locus_tag="IW687_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0949479.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83907..85298
                     /locus_tag="IW687_000183"
     CDS             83907..85298
                     /locus_tag="IW687_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0949480.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85371..86018)
                     /locus_tag="IW687_000184"
     CDS             complement(85371..86018)
                     /locus_tag="IW687_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0949481.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86026..86712)
                     /locus_tag="IW687_000185"
     CDS             complement(86026..86712)
                     /locus_tag="IW687_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949482.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86718..87791)
                     /locus_tag="IW687_000186"
     CDS             complement(86718..87791)
                     /locus_tag="IW687_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0949483.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88013..88687
                     /locus_tag="IW687_000187"
     CDS             88013..88687
                     /locus_tag="IW687_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949484.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88692..90032
                     /locus_tag="IW687_000188"
     CDS             88692..90032
                     /locus_tag="IW687_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0949485.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90181..91167)
                     /locus_tag="IW687_000189"
     CDS             complement(90181..91167)
                     /locus_tag="IW687_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0949486.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91891..92922
                     /locus_tag="IW687_000190"
     CDS             91891..92922
                     /locus_tag="IW687_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0949487.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93031..>93454
                     /locus_tag="IW687_000191"
     CDS             93031..>93454
                     /locus_tag="IW687_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0949488.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29896 a        16574 c        18964 g        28020 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca atcacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat
    62761 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    62821 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    62881 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    62941 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    63001 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    63061 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    63121 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    63181 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    63241 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    63301 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    63361 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    63421 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    63481 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    63541 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    63601 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    63661 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    63721 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    63781 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    63841 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    63901 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    63961 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    64021 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    64081 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    64141 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    64201 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    64261 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    64321 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    64381 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    64441 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    64501 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    64561 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    64621 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    64681 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    64741 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    64801 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    64861 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    64921 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    64981 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    65041 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    65101 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    65161 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    65221 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    65281 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    65341 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    65401 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    65461 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    65521 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    65581 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    65641 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    65701 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    65761 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    65821 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    65881 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    65941 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    66001 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    66061 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    66121 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    66181 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    66241 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    66301 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    66361 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
    66421 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    66481 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    66541 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    66601 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    66661 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    66721 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    66781 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    66841 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    66901 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    66961 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    67021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    67081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    67141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    67201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    67261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    67321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    67381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    67441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    67501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    67561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    67621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    67681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    67741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    67801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    67861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    67921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    67981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    68041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    68101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    68161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
    68221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    68281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    68341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    68401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    68461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    68521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    68581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    68641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    68701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    68761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    68821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    68881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    68941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    69001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    69061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    69121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    69181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    69241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    69301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    69361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    69421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    69481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    69541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    69601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    69661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    69721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    69781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    69841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    69901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    69961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    70021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    70081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    70141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    70201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    70261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    70321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    70381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    70441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    70501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    70561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    70621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    70681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    70741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    70801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    70861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    70921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    70981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    71041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    71101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    71161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    71221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    71281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    71341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    71401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    71461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    71521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    71581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    71641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    71701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    71761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    71821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    71881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    71941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    72001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    72061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    72121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    72181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    72241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    72301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    72361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    72421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    72481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    72541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    72601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    72661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    72721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    72781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    72841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    72901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    72961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    73021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    73081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    73141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    73201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    73261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    73321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    73381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    73441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    73501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    73561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    73621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    73681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    73741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    73801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    73861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    73921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    73981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    74041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    74101 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    74161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    74221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    74281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    74341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    74401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    74461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    74521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    74581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    74641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    74701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    74761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    74821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    74881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    74941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    75001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    75061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    75121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    75181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    75241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    75301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    75361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    75421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    75481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    75541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    75601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    75661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    75721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    75781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    75841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    75901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    75961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    76021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    76081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    76141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    76201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    76261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    76321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    76381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    76441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    76501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    76561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    76621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    76681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    76741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    76801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    76861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    76921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    76981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    77041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    77101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    77161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    77221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    77281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    77341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    77401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    77461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    77521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    77581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    77641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    77701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    77761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    77821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    77881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    77941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    78001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    78061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    78121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    78181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    78241 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    78301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    78361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    78421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    78481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    78541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    78601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    78661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    78721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    78781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    78841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    78901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    78961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    79021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    79081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    79141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    79201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    79261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    79321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    79381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    79441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    79501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    79561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    79621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    79681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    79741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    79801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    79861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    79921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    79981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    80041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    80101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    80161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    80221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    80281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    80341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    80401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    80461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    80521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    80581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    80641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    80701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    80761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    80821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    80881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    80941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    81001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    81061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    81121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    81181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    81241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    81301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    81361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    81421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    81481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    81541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    81601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    81661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    81721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    81781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    81841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    81901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    81961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    82021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    82081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    82141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    82201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    82261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    82321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    82381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    82441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    82501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    82561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    82621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    82681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    82741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    82801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    82861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    82921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    82981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    83041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    83101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    83161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    83221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    83281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    83341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    83401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    83461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    83521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    83581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    83641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    83701 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    83761 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    83821 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    83881 ggttgttgga acgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg
    83941 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    84001 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    84061 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    84121 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    84181 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    84241 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    84301 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    84361 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    84421 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    84481 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    84541 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    84601 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    84661 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    84721 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    84781 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    84841 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    84901 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    84961 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    85021 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    85081 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    85141 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    85201 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    85261 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    85321 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    85381 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    85441 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    85501 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    85561 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    85621 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    85681 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    85741 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    85801 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    85861 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    85921 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    85981 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    86041 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    86101 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    86161 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    86221 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    86281 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    86341 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    86401 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    86461 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
    86521 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
    86581 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
    86641 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
    86701 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
    86761 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
    86821 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
    86881 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
    86941 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
    87001 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
    87061 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
    87121 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
    87181 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
    87241 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
    87301 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
    87361 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
    87421 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
    87481 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
    87541 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
    87601 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
    87661 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
    87721 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
    87781 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
    87841 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
    87901 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    87961 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    88021 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    88081 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    88141 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    88201 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    88261 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    88321 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    88381 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    88441 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    88501 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    88561 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    88621 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
    88681 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    88741 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    88801 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    88861 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    88921 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    88981 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    89041 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    89101 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    89161 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    89221 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    89281 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    89341 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    89401 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    89461 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    89521 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    89581 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
    89641 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
    89701 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    89761 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
    89821 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    89881 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    89941 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
    90001 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    90061 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
    90121 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
    90181 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
    90241 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
    90301 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
    90361 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
    90421 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
    90481 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
    90541 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
    90601 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
    90661 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
    90721 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
    90781 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
    90841 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
    90901 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
    90961 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
    91021 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
    91081 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
    91141 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
    91201 gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca aatatttaga
    91261 ttggaatttg attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca
    91321 tattctaata aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt
    91381 ccactcgttg tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa
    91441 aaggattaat tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg
    91501 aagtaccaac tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac
    91561 cattattgaa taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa
    91621 ataaaagaaa gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt
    91681 aaccacatag ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat
    91741 aaaataacta aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat
    91801 ttgcttccta agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata
    91861 gaaaatgaac actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa
    91921 gaaaagttaa agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa
    91981 aatcgttctt ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt
    92041 actgattatt tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta
    92101 gaaaaacaag attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa
    92161 actacagcga accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg
    92221 aagcaatatt cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa
    92281 gagatttctc ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc
    92341 gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca
    92401 agcagagaag ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt
    92461 cttctgtatt gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct
    92521 ttgaccaagc agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata
    92581 ccaaaagaag cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac
    92641 gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc
    92701 caaattttga gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca
    92761 tatcgttatt atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt
    92821 aatcgatacc tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct
    92881 tatcacgaat tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt
    92941 ttttcacaat aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta
    93001 tattacttaa caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg
    93061 aacttattta cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt
    93121 gatgagccac tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc
    93181 gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa
    93241 gttattattt ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac
    93301 cttggtgcag tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt
    93361 atcgatcgtt tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga
    93421 gaaatttcaa atggaacaaa atcaaaacag attc
//
LOCUS       DACELF010000003        69736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.003, whole genome shotgun sequence.
ACCESSION   DACELF010000003 DACELF010000000
VERSION     DACELF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.003"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            336..1439
                     /locus_tag="IW687_000192"
     CDS             336..1439
                     /locus_tag="IW687_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0949489.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IW687_000193"
     CDS             1723..2403
                     /locus_tag="IW687_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0949490.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IW687_000194"
     CDS             2493..3527
                     /locus_tag="IW687_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0949491.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IW687_000195"
     CDS             3541..3876
                     /locus_tag="IW687_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949492.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IW687_000196"
     CDS             3860..6340
                     /locus_tag="IW687_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949493.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IW687_000197"
     CDS             6396..7955
                     /locus_tag="IW687_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0949494.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IW687_000198"
     CDS             7952..9316
                     /locus_tag="IW687_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0949495.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IW687_000199"
     CDS             9487..10557
                     /locus_tag="IW687_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949496.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IW687_000200"
     CDS             10572..11240
                     /locus_tag="IW687_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949497.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IW687_000201"
     CDS             11260..11838
                     /locus_tag="IW687_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0949498.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IW687_000202"
     CDS             12241..13152
                     /locus_tag="IW687_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0949499.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IW687_000203"
     CDS             complement(13408..14325)
                     /locus_tag="IW687_000203"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0949500.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IW687_000204"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IW687_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0949501.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15570
                     /locus_tag="IW687_000205"
     CDS             15022..15570
                     /locus_tag="IW687_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0949502.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15685..18045
                     /locus_tag="IW687_000206"
     CDS             15685..18045
                     /locus_tag="IW687_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0949503.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18132..18446
                     /gene="trxA"
                     /locus_tag="IW687_000207"
     CDS             18132..18446
                     /gene="trxA"
                     /locus_tag="IW687_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0949504.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18628..20406
                     /gene="uvrC"
                     /locus_tag="IW687_000208"
     CDS             18628..20406
                     /gene="uvrC"
                     /locus_tag="IW687_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0949505.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20551..21450
                     /locus_tag="IW687_000209"
     CDS             20551..21450
                     /locus_tag="IW687_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949506.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21443..22705
                     /locus_tag="IW687_000210"
     CDS             21443..22705
                     /locus_tag="IW687_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949507.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22868..24766
                     /locus_tag="IW687_000211"
     CDS             22868..24766
                     /locus_tag="IW687_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0949508.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25065..25784)
                     /locus_tag="IW687_000212"
     CDS             complement(25065..25784)
                     /locus_tag="IW687_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0949509.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26121..26453
                     /locus_tag="IW687_000213"
     CDS             26121..26453
                     /locus_tag="IW687_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949510.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26440..28443
                     /locus_tag="IW687_000214"
     CDS             26440..28443
                     /locus_tag="IW687_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0949511.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28462..28932
                     /locus_tag="IW687_000215"
     CDS             28462..28932
                     /locus_tag="IW687_000215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0949512.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28953..29537
                     /locus_tag="IW687_000216"
     CDS             28953..29537
                     /locus_tag="IW687_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0949513.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29552..30538
                     /locus_tag="IW687_000217"
     CDS             29552..30538
                     /locus_tag="IW687_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0949514.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30528..30839
                     /locus_tag="IW687_000218"
     CDS             30528..30839
                     /locus_tag="IW687_000218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0949515.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30861..32642
                     /locus_tag="IW687_000219"
     CDS             30861..32642
                     /locus_tag="IW687_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0949516.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32635..34011
                     /locus_tag="IW687_000220"
     CDS             32635..34011
                     /locus_tag="IW687_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0949517.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34018..34653
                     /locus_tag="IW687_000221"
     CDS             34018..34653
                     /locus_tag="IW687_000221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0949518.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34683..36038
                     /locus_tag="IW687_000222"
     CDS             34683..36038
                     /locus_tag="IW687_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0949519.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36230..37624)
                     /locus_tag="IW687_000223"
     CDS             complement(36230..37624)
                     /locus_tag="IW687_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0949520.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37716..38687
                     /locus_tag="IW687_000224"
     CDS             37716..38687
                     /locus_tag="IW687_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0949521.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38684..39466
                     /locus_tag="IW687_000225"
     CDS             38684..39466
                     /locus_tag="IW687_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0949522.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39635..40006)
                     /locus_tag="IW687_000226"
     CDS             complement(39635..40006)
                     /locus_tag="IW687_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0949523.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40140..40583
                     /locus_tag="IW687_000227"
     CDS             40140..40583
                     /locus_tag="IW687_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0949524.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IW687_000228"
     CDS             complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IW687_000228"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0949525.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41454..42821)
                     /locus_tag="IW687_000229"
     CDS             complement(41454..42821)
                     /locus_tag="IW687_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0949526.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42805..43635)
                     /locus_tag="IW687_000230"
     CDS             complement(42805..43635)
                     /locus_tag="IW687_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0949527.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43798..44235
                     /locus_tag="IW687_000231"
     CDS             43798..44235
                     /locus_tag="IW687_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0949528.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44311..44931
                     /locus_tag="IW687_000232"
     CDS             44311..44931
                     /locus_tag="IW687_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0949529.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45120..45626
                     /locus_tag="IW687_000233"
     CDS             45120..45626
                     /locus_tag="IW687_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0949530.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45643..46542
                     /locus_tag="IW687_000234"
     CDS             45643..46542
                     /locus_tag="IW687_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0949531.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46629..47513
                     /locus_tag="IW687_000235"
     CDS             46629..47513
                     /locus_tag="IW687_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0949532.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47705..47776)
                     /locus_tag="IW687_000236"
     tRNA            complement(47705..47776)
                     /locus_tag="IW687_000236"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47741..47743),aa:Arg,seq:ccg)
     gene            47836..49170
                     /gene="rpoN"
                     /locus_tag="IW687_000237"
     CDS             47836..49170
                     /gene="rpoN"
                     /locus_tag="IW687_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0949533.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49373..50410
                     /locus_tag="IW687_000238"
     CDS             49373..50410
                     /locus_tag="IW687_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0949534.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50451..51452
                     /gene="gap"
                     /locus_tag="IW687_000239"
     CDS             50451..51452
                     /gene="gap"
                     /locus_tag="IW687_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0949535.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51562..52752
                     /locus_tag="IW687_000240"
     CDS             51562..52752
                     /locus_tag="IW687_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0949536.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52856..53611
                     /locus_tag="IW687_000241"
     CDS             52856..53611
                     /locus_tag="IW687_000241"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0949537.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53727..55025
                     /gene="eno"
                     /locus_tag="IW687_000242"
     CDS             53727..55025
                     /gene="eno"
                     /locus_tag="IW687_000242"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0949538.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55425..56564
                     /locus_tag="IW687_000243"
     CDS             55425..56564
                     /locus_tag="IW687_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0949539.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(56631..57206)
                     /locus_tag="IW687_000244"
     CDS             complement(56631..57206)
                     /locus_tag="IW687_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0949540.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(57211..58185)
                     /locus_tag="IW687_000245"
     CDS             complement(57211..58185)
                     /locus_tag="IW687_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0949541.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(58209..58253)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            58468..59436
                     /locus_tag="IW687_000246"
     CDS             58468..59436
                     /locus_tag="IW687_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0949542.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            59549..59827
                     /locus_tag="IW687_000247"
     CDS             59549..59827
                     /locus_tag="IW687_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0949543.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            59945..60181
                     /gene="secG"
                     /locus_tag="IW687_000248"
     CDS             59945..60181
                     /gene="secG"
                     /locus_tag="IW687_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0949544.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            60293..61039
                     /locus_tag="IW687_000249"
     CDS             60293..61039
                     /locus_tag="IW687_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0949545.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            61042..63399
                     /gene="rnr"
                     /locus_tag="IW687_000250"
     CDS             61042..63399
                     /gene="rnr"
                     /locus_tag="IW687_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0949546.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            63421..63885
                     /gene="smpB"
                     /locus_tag="IW687_000251"
     CDS             63421..63885
                     /gene="smpB"
                     /locus_tag="IW687_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0949547.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            64187..64906
                     /gene="atpB"
                     /locus_tag="IW687_000252"
     CDS             64187..64906
                     /gene="atpB"
                     /locus_tag="IW687_000252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0949548.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            64959..65174
                     /gene="atpE"
                     /locus_tag="IW687_000253"
     CDS             64959..65174
                     /gene="atpE"
                     /locus_tag="IW687_000253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0949549.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            65265..65789
                     /gene="atpF"
                     /locus_tag="IW687_000254"
     CDS             65265..65789
                     /gene="atpF"
                     /locus_tag="IW687_000254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0949550.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            65776..66318
                     /locus_tag="IW687_000255"
     CDS             65776..66318
                     /locus_tag="IW687_000255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0949551.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            66341..67897
                     /locus_tag="IW687_000256"
     CDS             66341..67897
                     /locus_tag="IW687_000256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0949552.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            67911..68813
                     /locus_tag="IW687_000257"
     CDS             67911..68813
                     /locus_tag="IW687_000257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0949553.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            68836..>69736
                     /gene="atpD"
                     /locus_tag="IW687_000258"
     CDS             68836..>69736
                     /gene="atpD"
                     /locus_tag="IW687_000258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0949554.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSI"
BASE COUNT        23160 a        12191 c        14804 g        19581 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccaatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    15601 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    15661 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    15721 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    15781 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    15841 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    15901 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    15961 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    16021 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    16081 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    16141 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    16201 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    16261 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    16321 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    16381 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    16441 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    16501 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    16561 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    16621 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    16681 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    16741 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    16801 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    16861 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    16921 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    16981 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    17041 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    17101 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    17161 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    17221 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    17281 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    17341 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    17401 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    17461 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    17521 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    17581 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    17641 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    17701 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    17761 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    17821 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    17881 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    17941 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    18001 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    18061 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    18121 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    18181 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    18241 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    18301 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    18361 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    18421 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    18481 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    18541 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    18601 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    18661 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    18721 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    18781 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    18841 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    18901 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    18961 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    19021 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    19081 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    19141 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    19201 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    19261 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    19321 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    19381 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    19441 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    19501 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    19561 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    19621 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    19681 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    19741 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    19801 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    19861 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    19921 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    19981 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    20041 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    20101 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    20161 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    20221 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    20281 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    20341 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    20401 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    20461 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    20521 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    20581 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    20641 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    20701 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    20761 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    20821 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    20881 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    20941 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    21001 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    21061 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    21121 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    21181 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    21241 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    21301 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    21361 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    21421 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    21481 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    21541 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    21601 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    21661 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    21721 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    21781 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    21841 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    21901 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    21961 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    22021 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    22081 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    22141 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    22201 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    22261 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    22321 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    22381 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    22441 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    22501 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    22561 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    22621 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    22681 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    22741 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    22801 atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg
    22861 cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt
    22921 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    22981 tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    23041 aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag
    23101 ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa
    23161 acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa
    23221 atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    23281 actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga
    23341 acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca
    23401 aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc
    23461 agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca
    23521 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg
    23581 gattgccgca cgctacaact atttatttga attagagaaa gaatatggat taaatttacg
    23641 tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa
    23701 caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc
    23761 tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga
    23821 cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca
    23881 gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt
    23941 cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag
    24001 aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    24061 gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga
    24121 attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga
    24181 aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg
    24241 gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt
    24301 tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga
    24361 agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat
    24421 catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg
    24481 aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc
    24541 tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt
    24601 gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga
    24661 gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca
    24721 gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc
    24781 aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg
    24841 aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact
    24901 caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc
    24961 ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    25021 actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca
    25081 aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat
    25141 tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg
    25201 ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag
    25261 gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg
    25321 acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt
    25381 ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt
    25441 cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt
    25501 tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa
    25561 agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt
    25621 tgaggcgctg aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa
    25681 actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc
    25741 gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa
    25801 gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac
    25861 ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga
    25921 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    25981 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    26041 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    26101 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    26161 cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag
    26221 aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt
    26281 tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga
    26341 aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc
    26401 aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    26461 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    26521 atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa
    26581 aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat
    26641 cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    26701 aagaaactcc atttgaaaca tcaagaactt tccttacaag acttggaagc aaattattcc
    26761 gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag
    26821 ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    26881 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    26941 agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg
    27001 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    27061 gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    27121 gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag
    27181 cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa
    27241 tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc
    27301 catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    27361 atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca
    27421 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa
    27481 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg
    27541 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac
    27601 ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg
    27661 atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg
    27721 atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct
    27781 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    27841 ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa
    27901 gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt
    27961 attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg
    28021 ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa
    28081 gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat
    28141 ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca
    28201 ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg
    28261 atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt
    28321 gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt
    28381 gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa
    28441 taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta
    28501 tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt
    28561 gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga
    28621 caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc
    28681 tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta
    28741 ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt
    28801 gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    28861 tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta
    28921 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    28981 agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga
    29041 tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag
    29101 aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag
    29161 aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg
    29221 caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat
    29281 tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt
    29341 atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa
    29401 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    29461 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    29521 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    29581 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    29641 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    29701 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    29761 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt
    29821 ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta
    29881 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    29941 aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    30001 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    30061 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    30121 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    30181 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    30241 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    30301 ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta
    30361 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    30421 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    30481 aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    30541 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    30601 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    30661 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    30721 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    30781 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    30841 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    30901 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    30961 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    31021 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    31081 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    31141 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    31201 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    31261 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    31321 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    31381 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    31441 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    31501 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    31561 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    31621 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    31681 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    31741 atacatcaaa catgccagtg gcagctcgag aagcttctat ctatacggga atcacaattg
    31801 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    31861 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    31921 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    31981 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    32041 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    32101 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    32161 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    32221 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    32281 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    32341 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    32401 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    32461 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    32521 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    32581 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    32641 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    32701 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    32761 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    32821 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    32881 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    32941 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    33001 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    33061 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    33121 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    33181 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    33241 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    33301 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    33361 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    33421 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    33481 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    33541 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    33601 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    33661 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    33721 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    33781 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    33841 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    33901 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    33961 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    34021 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    34081 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    34141 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    34201 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    34261 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    34321 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    34381 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    34441 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    34501 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    34561 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    34621 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    34681 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    34741 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    34801 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    34861 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    34921 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    34981 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    35041 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta
    35101 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    35161 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    35221 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    35281 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    35341 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    35401 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    35461 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    35521 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    35581 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    35641 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    35701 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    35761 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    35821 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    35881 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    35941 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    36001 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    36061 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    36121 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    36181 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    36241 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    36301 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    36361 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    36421 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    36481 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    36541 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    36601 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    36661 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    36721 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    36781 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    36841 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    36901 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    36961 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    37021 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    37081 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    37141 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    37201 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    37261 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    37321 cttcattggt agagatggtg atggactcaa agacattggg agaattaaat gtgccacttc
    37381 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    37441 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    37501 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    37561 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    37621 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    37681 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    37741 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    37801 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    37861 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    37921 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    37981 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    38041 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    38101 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    38161 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    38221 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    38281 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    38341 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    38401 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    38461 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    38521 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    38581 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    38641 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    38701 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    38761 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    38821 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    38881 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    38941 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    39001 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    39061 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    39121 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    39181 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    39241 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    39301 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    39361 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    39421 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    39481 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    39541 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    39601 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    39661 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    39721 cagtaatctc aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    39781 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    39841 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    39901 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    39961 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    40021 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    40081 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    40141 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    40201 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    40261 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    40321 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    40381 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    40441 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    40501 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    40561 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    40621 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    40681 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    40741 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    40801 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    40861 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    40921 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    40981 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    41041 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    41101 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    41161 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    41221 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    41281 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    41341 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    41401 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    41461 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    41521 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    41581 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    41641 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    41701 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    41761 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    41821 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    41881 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    41941 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    42001 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    42061 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    42121 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    42181 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    42241 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    42301 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    42361 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    42421 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    42481 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    42541 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    42601 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    42661 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    42721 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    42781 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    42841 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    42901 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    42961 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    43021 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    43081 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    43141 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    43201 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    43261 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    43321 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    43381 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    43441 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    43501 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    43561 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    43621 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    43681 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    43741 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    43801 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    43861 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    43921 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    43981 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    44041 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    44101 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagg agggaaggtt
    44161 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    44221 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    44281 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    44341 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    44401 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    44461 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    44521 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    44581 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    44641 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    44701 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    44761 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    44821 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    44881 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    44941 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    45001 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    45061 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    45121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    45181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    45241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    45301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    45361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    45421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    45481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    45541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    45601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    45661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    45721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    45781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    45841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    45901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    45961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    46021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    46081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    46141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    46201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    46261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    46321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    46381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    46441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    46501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    46561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    46621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    46681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    46741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    46801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    46861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    46921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    46981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    47041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    47101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    47161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    47221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    47281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    47341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    47401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    47461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    47521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    47581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
    47641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
    47701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
    47761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
    47821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
    47881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
    47941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
    48001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
    48061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
    48121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
    48181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
    48241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
    48301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
    48361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
    48421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
    48481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
    48541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
    48601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
    48661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
    48721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
    48781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
    48841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
    48901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
    48961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
    49021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
    49081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
    49141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
    49201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
    49261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
    49321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgttgga
    49381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
    49441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
    49501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
    49561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
    49621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
    49681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
    49741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
    49801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
    49861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
    49921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
    49981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
    50041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
    50101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
    50161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
    50221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
    50281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
    50341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
    50401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
    50461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
    50521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
    50581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
    50641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
    50701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
    50761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
    50821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
    50881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
    50941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
    51001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
    51061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
    51121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
    51181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
    51241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
    51301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
    51361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
    51421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
    51481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
    51541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
    51601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
    51661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
    51721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
    51781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
    51841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
    51901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
    51961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
    52021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
    52081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
    52141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
    52201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
    52261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
    52321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
    52381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
    52441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
    52501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
    52561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
    52621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
    52681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
    52741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
    52801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
    52861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
    52921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
    52981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
    53041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
    53101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
    53161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
    53221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
    53281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
    53341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
    53401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
    53461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
    53521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
    53581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
    53641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
    53701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
    53761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
    53821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
    53881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
    53941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
    54001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
    54061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
    54121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
    54181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
    54241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
    54301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
    54361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
    54421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
    54481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
    54541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
    54601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
    54661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
    54721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
    54781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
    54841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
    54901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
    54961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    55021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    55081 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttttttt
    55141 gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa
    55201 aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga acagtagaat
    55261 aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt
    55321 ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag
    55381 cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa
    55441 aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag
    55501 tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt
    55561 acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt
    55621 taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg
    55681 caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga
    55741 agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat
    55801 ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg
    55861 aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa
    55921 ctaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg
    55981 gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg
    56041 aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg
    56101 tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca
    56161 tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata
    56221 aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt
    56281 tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga
    56341 ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa
    56401 cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg
    56461 gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata
    56521 tgattgaaaa atatcaagga attattttat cctacttagc gtaagatttt tttaataaat
    56581 gaaaaaacag agccagatta gcattatttt gctaatccag ctctgtttat tcagcctatt
    56641 tttctttttg aatatatcaa gcgttgtggc aacatagtga tgactaatga aacaacgacg
    56701 atagacaaca aacgatcaag gtagtctgta cctgcctgta ccaagatcac acttgttgtc
    56761 tgacttaaac ctaacccatg taatagctga acgatcatac tggatcctga ggaggtgatc
    56821 ccgccaaata actgaacggt aatgatcgaa gaaacgatcg ttccaggtac cgacaaaatc
    56881 aaagcgagta ataacagttt caagctgttt ttatctttgc cgagatatct tggcaaaatc
    56941 aatccagcaa gcaagcctgt gagaatttgg attggcgaat aatacagaga aaaaatatct
    57001 gtagtcactc cacttaaaat accgctggac aatcctgtca gcattccagg tacaggccca
    57061 agcaatgcac cagcaaaaat cgttccgata gaatcgagat agattggcaa acgaagccat
    57121 aaagcaatcg ttcctccaat ataattgatc ccaatacaca aagccatcac tgtaagtacg
    57181 cgagttgcca ttttgttttt attcattttg ttagcccatc tttaaccttt ccaaatcttt
    57241 cataatcgtt tcttcttgtg catttaaaag aacggttaaa aactttcgga aaaaaagacg
    57301 agtatctact tttgtcataa ttttactgtt agcaggtttc tgccaaaatt cataacgatc
    57361 aaccaatgtt tgtccacgac ttattccttg agtttccaca gcagtgaagc ttttgaaacc
    57421 ttcacagatg gccccctcga taaaataagc aacagctaac ggatcattga tgacacatcc
    57481 taaaattctt tcttgtttcc agtggaagtc aaaataaaaa cgagtgatcg cttttagata
    57541 ctctccctct tcaggattca tttgacagca gtattcgagg atagtcggtg tcaagacgat
    57601 ttctcttgtc acatctaacc caaccatttc tatcgtttgt ttgagattct cgaatacata
    57661 agaagctgct tcaggatcac accagtaatt gtattcagca actggcgaac agttaccatg
    57721 gcttttgtaa gttccaccca tcgagacaaa acgatccata tgtttcccaa tatttgggtt
    57781 gactttcaaa gcaaaagcga tattggtcaa aggacccaaa gcaatgactg atgtatttgt
    57841 cttttctttg aaataatcag ccaaaaaatc aacggcatgg agaggttctg cctgttttct
    57901 caagatcatc gggaaatttg tttcccctaa accatccata ccatgagtgt cttgtgcact
    57961 gatgaaaggt tgcttcaaag gtttttccat tccttgataa acaggaatgt ctaaacgatc
    58021 acagcgttcc aggcatttca gcacattctg cgtacctata tctgcaggga cattcccaca
    58081 aactacggta atagcaacta cttcgatatc aggggatttg acagctaatg ataaagctaa
    58141 agtgtcgtcg atccctggat cgcaatcaat aattactttt cgcattattt ccaatctcct
    58201 ttctttagag atacttagtt ttttattctg ggaagtttcc gaaccagtcc aagacgagcg
    58261 tcttgattat aatgcacgaa attagtatac tggcttttat tgcgaatgta aatgctctct
    58321 tacaagtaaa tctattgagg agaactttaa gtaaatgata caatgaaaaa gaaatggaat
    58381 ttacgtgtgc cgaaaataaa gaatcctatg gatcggtaca ttggataata gttagattat
    58441 agtttggcag aaagcggcgg gtgtacaatg gttatttttt atttcattct tattctgata
    58501 attataggga tcagttacca ttttctttct tatttttatg caaagaaatt agtaagagcg
    58561 ggagtacaga gaggatatga ctggtatgaa gacactggtc atcgtttgat ggaccctgct
    58621 gcagtcacgt cagtagaaaa aaaacgacaa aaattagaag aagagtgtga aagcttttgg
    58681 gagcagggaa cacccaaaac aatcaaaagt tatgacgggt tgaaactagc tggtcagatg
    58741 ttcatccatc caaataagca gaataaatgg gtcatttgcg tacatgatta ccgaagcaca
    58801 gggaaaagag acatgtctca tataggtaaa agatatgcag agaaaggatt caacgttctt
    58861 gtacctgatt tacgtgcgca cggagaaagt gaaggagaaa tcatcggaat gggatggcta
    58921 gatcggctag atttaattgc ctggatccaa ctcatcttag atgaacagcc gaatgcgtcc
    58981 attattttac acggcggatc aatgggagct tctacgatca tgatggcaag tggtgaaaaa
    59041 cttccatcag cggtcaaagg atttatttta gacagcggat atgtttctgt ttatgcagaa
    59101 ttccgatata tgttaagtaa aatcactgtc tttcctaaaa aaatggtcat gagatatgcg
    59161 aatcattatg cccagaaata cgccggttac tccttaaagc aagcatcagc tacgagacag
    59221 cttggcagca accatctgcc tttattggtc attcatggtg aaagagatca ttttgtccct
    59281 acagaagcag cgtacacgat tcaaaatgct acggcagggg ataaagcact gctgcttgtt
    59341 ccagaagcag aacacttgga agcatcgatg aaagatccga aaacctattg gacagtgatt
    59401 ttttcattta ttgaacaaag aataacgatg aattaatttt ctcttctttt ttgtaagtaa
    59461 ttcgaggata aataattgtt tatagatcag gagactgcta aaataaggta ggaataaaaa
    59521 aatatcagtt atttgaggag tgcggcatat ggaaatcaaa gaagaaaaaa atcgtcttgc
    59581 tttattcaat gatgaacaac aagagatcgg agaaatgaca tggtcagatg cgggcccaga
    59641 catcatgatc attgaccata catttgtaga tccagcatat cgtggtcaaa agttagctga
    59701 aaagctagtg tatactggag tagaattagc aagacgagac gggaaaaaga tcattccttt
    59761 atgcccttat gcaaaaaaag agtttgaaaa gaaaccagaa tatcaagacg tcttacgtca
    59821 gtcataagaa taacagctga aagttttgaa ctttcagctg ttattccatt tatatagagt
    59881 tgtcacggac ttttatctat gataaagtta aagagacgat atcttttggg aaggaggctg
    59941 cgatatgtat aatttaattt tgggaatcgt gatcgtttta tcgattgtta tggtcattgc
    60001 gattatgatg cagccaagca agcaaaatag tgcagcgagt gcatttaccg gcggtgcgga
    60061 tcaactattc ggcaaacaaa aagctcgcgg ctttgaagct gtcatgcagc gatcaactgc
    60121 agttatggga gctatctgga tgatcttatt atttgtccta gcatatttat cttcgaagta
    60181 gttgaataag cctctcgcaa aacggcgggc ggcttttttt attgcaaaga gaacgcttaa
    60241 gaagaagtta tgaaagaaac tatggtaaaa tgaggcgaaa gaggtgagac gagtgagaca
    60301 attaccaaaa cccctttatg caaaacatgg aaagcgagcg gttttgctgc ttcacgctta
    60361 ctcaggaagt ccaaacgatg ttcgaatgct cgcacgattt ttggaaaaag cagattatac
    60421 agtttatgcg ccgcttttta aaggacacgg aacaatggag ccatatgata ttttgcaaga
    60481 aaaagcagaa agctggtggg cagacactaa aaaagccatc cattttttac agtctgagca
    60541 gttttctgat attgccgtat taggattatc aatgggcggc atctttgctg tacgagcact
    60601 agaagaagaa tcagtgattg gcggcggttt tttctgttcg cctttgtctc ctgtaaaaac
    60661 aaacgtgcct gaaaactttg aaaaatacgt acgtcaagtg ctgaagacag caggtaaatc
    60721 agagaaagaa atcaatgaaa aagcagttgc ttaccgctcg ttagctgaac aacaattaat
    60781 ggatatacaa gatcaggcag caatcgtaga aagtaggttg tcagatatcc aacagccgat
    60841 atttttagct caagctggga aagatgaaat gatcgaccct aatggcgtat ttgaaactgc
    60901 acgcaagttg agccgtcagc gagtgacact tcgatggtat cctgaaagtg gtcatgtgat
    60961 tacagttggt acagcaagac gcgaattaga aaaagacgtc ttggaatttt tagaaaaatt
    61021 accttggaat gaggaataaa tatgacaaaa gaaacgataa aagagaaaat tctctttttc
    61081 atggaaagca gtcgcaaacg cagtttttct atggaagaaa tcgctgaagg tttagggttc
    61141 caaaaaagcg atgattttaa attactagta caaacgattg ctcaaatgga aagagaacag
    61201 agtatagttt tcaataaaaa aggaaaagtg aaacttcctt taaaaccaat cttgatcgaa
    61261 ggaacatttc gagcaaatga acgaggattt ggatttgtaa cgattgatcc cgaagaagat
    61321 gacatctata tcccaaaaga agcgactggt tttgcaatgg atggagacac cgtagcaatc
    61381 gatatcatga agcgagcaaa tacagatatg gatcgcggag cagaggggaa agtagtcgag
    61441 attcgagacc gggcgactac tcagttagca ggggaattcg ttgcttattc agaagaagaa
    61501 atacaagaaa cggatcttta cggcgttgtt atcccgaaag atagaaagct caaccaattt
    61561 aaagtatatg cagcagcaga gggagttcgt ccagttgacg gaagtattgt gctgatcgaa
    61621 ctgacccatt accctgaaaa agggtatgcg actagtttag aagggataat caagcaagtg
    61681 attggtcata agaatgaccc tggcatggat atcctctcaa ttgtcgtggc taatggtatc
    61741 cctacgaaat ttcctgatga tgtattgtct gaagcagatg aagttccaga tcaaatcagc
    61801 gaaaaggata taactggacg tagagattta agaaatcagc tcattgttac gattgatgga
    61861 gaagatgcaa aagatctaga tgatgcagtt acggttaaaa aattagataa cggtaattat
    61921 tttcttggag tacacattgc agatgtttct tactatgtga cacaaggcag tcaattggac
    61981 agagaagcat atgaaagagg gacaagtgta tatttgactg atcgagtgat tccgatgatc
    62041 ccgcaacgtt tatcgaatgg tatctgttca ctgaatcccc atgttccaag gctgaccatg
    62101 agttgcgaaa tggagattga tccaaatgga caagtagtct ctcatgatat tttcccaagt
    62161 gtgatccaaa caacagaacg gatgacctac acagctgtca atcaaatctt agaagatcaa
    62221 gacgaagaaa cgatgcaacg ctatgctcag ctcgtaccta tgttccaaga aatgggtgaa
    62281 ttgcatcgaa tccttgaaac gatgcgaatc aaacgtggcg cgatttcttt tgaggatcgc
    62341 gaagcaaaaa tccttgttga tgctgaagga cacccaaatg atatccagct aagaagcaga
    62401 ggaattggtg aacgcttgat cgaatctttc atgcttgcgg ccaatgaaac ggtagctgaa
    62461 cattataata aattaaatct gccgtttatt taccgaatcc atgaacagcc aaaagaagaa
    62521 aaaatgcagc gcttctttga ttttgcttca gctttgggca ttctggtccg aggaacgaaa
    62581 ggaacgatca caccaaaaga tttacagcat gtgatcgaag gagtagaaga caaaccagaa
    62641 gctgcagtta tcaatactat gttgctaaga agcatgcaac aagcacgcta ttcagaagac
    62701 aattttgggc attatggctt agcggcagat tactacacac actttacatc tcctattcga
    62761 cgctatcccg atctaatcgt ccatcgtctg atccgttctt acagtgaaga taaaagtgaa
    62821 agcaatcaaa gttcgtgggc tacggaactt cctgaaattg cagatcatag ttctaagatg
    62881 gaacgtcgtg cagtagaaac agaacgtgaa gtagattcca tgaagaaagc agaattcatg
    62941 atggacaaag tgggggaaga attcgatggg atcatcagct ctgttgtgaa attcggcctg
    63001 tttatcgaac tgccgaatac agtagaggga ttgatccata tcaatgaatt gaaacaggac
    63061 tacttccatt ttgttgaaaa tcatttagct cttgtaggtg aaagaactgg tctgactttt
    63121 aaaatcggac agaaagtgcg agtaaaagta gtcaaagctg atccagatga acgagcagta
    63181 gattttgaac ttgtttccgc agaagaggtt acagcgttgg aacggccaaa aacacgaaat
    63241 ggaaaatcag gaaaacgcga aggaaaacaa gcaggaaaac gcccagaaag aaatggcaag
    63301 aagaacaaac gatatagcga tagtaagcaa acgttcggta agaaaaaagg gaagaagcca
    63361 ttttacaaag gtgttaaaaa gaagaaaaag aaaaaataaa atgagttgga ggaaacagct
    63421 atgccaaaag gcgagggaaa attaattgca caaaacaaga aagctcgcca tgattattcg
    63481 atcatcgaca cgatggaagc agggatggtc ttgcaaggaa ccgagatcaa gtcgatacga
    63541 aacagccgga tcaatctaaa agatggattt attcgcgtcc gcaacggaga agctttcttg
    63601 cataatgttc atatcagtcc ttatgaacaa ggaaatattt ttaatcatga tccgttgcgc
    63661 acgagaaagt tattattaca caaaaaacaa atcatccggc ttgaaaatga attgaaaaat
    63721 actggaatca ctgttgttcc tttaaaagtc tatattcgta acggctatgc caaggtattg
    63781 attggtctgg cgaaagggaa aaaatcttat gataaacggg aagatttgaa acgaaaagat
    63841 atcgatcgac aaattgatcg aacattaaaa aatttctcta gataatttca ttatcctttt
    63901 tcgcatattc ggtgcttttg cttttactta atgataaaat ttgttagcat ttaatctgat
    63961 aaaacatgat tatcataggt tttttcttag atcaggagca ccgtgtataa agaaaaattc
    64021 gcttcgtttt tgactgaaat aaaaaagtgt ctaattttca tgaaaatgtt ttgaattttc
    64081 tgtttcatgg tggtatgata ccgtgtggtt agaaaccgat tggaaaaaac cagatacgca
    64141 catgaattga ttttatgtga atttcgggga aaagaggtga aatggattgg atgaacgctc
    64201 gctaacgttc cacattggac caatttggtt tgatggtaca gtttgtataa tggtgttact
    64261 gacctgtttg attgttttct tcttagctta cttttttaca agaaatctta agttgaaacc
    64321 aacaggcaaa caaaatgcga tagaatgggt cattgatttt agtcgaggaa tcgtaacaga
    64381 taatctgcct agaaaagaat tgagcaattt ccatttgttg gcatttacat tgtttttatt
    64441 tgtttttgtt gcaaacaaca tcggtttagt aacaaaaatc gttttaccta acgaaacaac
    64501 actttggaaa agcccgacag cagacccgtt tgttacttta actttggcat ttataatgat
    64561 cttacttact cacttatttg gggtgaaaaa gttaggcttc aaaggatatt tcgtaaattc
    64621 gttcttgaaa ccatatagtt ttatgtttcc gatgaaactg attgaagaat ttacaaactt
    64681 attaacactt gctttacgtc tttatgggaa tatttatgcg ggggaagttt tgctaacctt
    64741 gattgcaaaa atggtaataa gcttaggctg gttctcattg ccgttagcaa ttccattaga
    64801 gatggtctgg atcgcattct ccttgtttat cggaagcatc caagcatttg tcttcgttac
    64861 tttatctatg gtttatatga gccataagat tgaagtggaa gaataatatt cgttttaaaa
    64921 acaaactact atttatctaa tttaggagga aaacaattat gaattatatc gcagcagcaa
    64981 tcgcaatcat gggagcagca atcggagccg gttatggtaa cggtcaagtt atctctaaaa
    65041 caatcgaatc aatggctcgc caacctgaaa tgtctggtca attccgtaca acaatgttta
    65101 tcggggtagc tctagtcgaa gctgttccta tcttaggggt agttgtcgcc ttgattctag
    65161 tttttggtgt ttaattgaat cagtattaag gcagaaacaa tttggtttgt tactgctttt
    65221 ttacatgaaa actagaagta cttcaaagaa aggaaggctc gcctatgctg aatcaattgg
    65281 caattgcaga agttggcaat gcgatgttag gaaatgtcat cgttgtcagc ggctcatttt
    65341 tgatactact ggctctcttg aagcattttg cttggggacc aatcagcgat attttgaaaa
    65401 aacgtgaaga caagatcgcc aatgatttgg attctgcaga acaatctcgc atcaactcag
    65461 cgaaaatgga acaagaacgc gaacaacaat tgttagcctc tcgttctgat gcagctgata
    65521 tcatcaaaaa tgcgaaagaa agtggagaat taagccgcca aaatattttg aaggatgctc
    65581 aagaagaagc agctcgtcta aaaagtaaag cccaagctga tatcactgta gaacgtgatt
    65641 cagcgctgaa ctctgtaaaa gacgatgttg cagaactatc tcttcaaatc gcggaaaaaa
    65701 tcttgaacaa agaattatct ccagaaatgc acgaatcatt aatcaatcaa tatatcgaag
    65761 gtctaggttc ttcaaatgaa actagataaa tatacagtag gaagacgtta cgggaaagct
    65821 ttatttgaac tggcgatcga ttcaaataaa gcagaagaaa tttatcaaga tctcgtaagc
    65881 ttacgtcaaa tctatgaaca agtacctgga ttaggtaata tgttaagtga tgttcgtcta
    65941 gaacctcatg aaaaacgaga aatcatggac aagcttgtta gtggctatga tggaatcgtt
    66001 aaaaactttt tagaagttgt ttatgcgtat aaccgaatgg atgatctttt gttcatgatc
    66061 gaagaatatg aacgtcgata tgatgaacat caaggtttgc tgttgggaag tgtcacaaca
    66121 gcagttccac tttcagacga acaacgaata caattagaaa agaatgttgc aaaaacaatg
    66181 aactatcaat cagtcgaatt aaaacaaatc gttgattctt ctattattgg tggcgcgatt
    66241 gttgaagcta accatcgagt aatcgacgga agcattcgca cgcaattaga aaatatgcgt
    66301 aatcaattaa atagatagta caaagaggta ggtgaatcga atggccatca aagcagaaga
    66361 aattagtgcc ttgattaaag aacaaattga aaattatcaa aacgtgcttt cagtcgaaga
    66421 aatcggtacc gtaacttatg taggtgacgg gatcgctcgt gcacatggtt tagaaaacgc
    66481 gatgagtggt gagttgcttg aattttcaaa tggctcatac ggaatggcgc aaaacttaga
    66541 atcaaatgat gtaggtatca ttatccttgg cgatttcgag tccattcgtg aaggagataa
    66601 agtaaaacga acaggaaaga tcatggaagt tccagttgga gaggccttga ttggacgggt
    66661 agttaatccg ctaggtcagc caattgacgg actaggtgaa atcgttactg acaaagctcg
    66721 tccagtagaa gcaatggcac caggcgttat gcaacgtaaa tctgttaacg aaccaatgca
    66781 aacaggtcta aaagcaatcg acgctcttgt accaatcgga cgcggccaac gtgaattagt
    66841 tatcggtgac cgtaaaacag ggaaaacttc cattgcgatc gatacgatca tcaaccaaaa
    66901 aggtcaagat atgatctgta tctatgtagc aatcggacaa aaagattcta cagttcgtac
    66961 acaagttgaa acattgaaaa aatatggcgc aatggattac acaatcgttg tgaatgccgg
    67021 tgcgtctcaa ccagcgccgt tgctttatat cgcaccatat gctggtactg caatgggtga
    67081 agagttcatg tacaacggga aacatgtctt gatcattttt gatgatcttt caaaacaagc
    67141 tgttgcttat cgtgaacttt ccttgctgct tcgccgtcca ccaggtcgtg aagcttatcc
    67201 aggggatgtt ttctacttac actcacgctt attggaacgt gcagcaaaat taagtgatga
    67261 tttaggtggc ggttcaatga ctgccttacc atttgttgaa acacaagctg gagatatctc
    67321 tgcttatatc ccaacaaacg ttatttcgat cactgatgga caaatcttct tagaaagcga
    67381 cttgttctac gcaggtactc gtccagcggt tgatgccgga ctatctgtat ctcgtgtagg
    67441 gggttctgcg caaatcaaag caatgaaaaa agttgctggg actttacgtc tagacttagc
    67501 aagttaccgt gaattagaag catttacaca attcggttct gacttggatg cagcaacaca
    67561 agcaaaacta aatcgtggac gccgtacagt agaaatcttg aaacaaaaat tgcatgcacc
    67621 gctagctgtt gaaaaacaag tagtgatttt gtatgctttg acacatggat tcttagatag
    67681 tatccctgta gacagtattt tagacttcga acatgaatta ttcgaatatc tggataccaa
    67741 tcattcagat atctttgaaa caatccgtac aacaaaagat cttccagaag aagaagcgtt
    67801 gaacagtgcg attcaggaat ataaagacat gttcttagct acaaaaggaa ataattcctc
    67861 tacagaagat aaattgaaat ccattcaaaa tgcatagtga ggtgagatga atgggtgctt
    67921 cattaaatga aatcaagaca cgtatcgctt caacgaaaaa aacgagccaa atcacgcggg
    67981 caatgcagat ggtgtctgct tctaagttga caaaatctga agcttcttca cagaagtttc
    68041 aggtttatgc aacaaaagta agagaaatcg ttacgcattt gactgctact cagttaaatg
    68101 atattgcctc agaaaatcct agaggggata tcaattacaa cagcatgttg ctcagccgtc
    68161 ctgttaagaa aacgggttat atcgtcatca ctgcagatgg cggtttagtc ggtggatata
    68221 acagctcgat attgaaacaa acgatgtcta ttttagaaga agaccaccaa tcacccgatg
    68281 attacgtgat gatcgcaatt ggcggaactg gtgcagactt ctttaaagca cgcggtatca
    68341 atctagcata tgagctgcgc aatctatctg atcagccaag ctttgatgaa gtccgcaaaa
    68401 tcgtaaatat ggcaacaact atgtaccaaa acgaagtatt cgatgaatta tacgtatgtt
    68461 ataaccatca tatcaattcg ctaactagtc agtttcgtgt agaaaaaatg ctgccaatct
    68521 cagacttaga tccagaagaa gcaactactt tcgaacaaga atatattttt gaaccttcaa
    68581 aagaagaaat cttgtctcag ttattgcctc agtatgctga aagtttgatc tacggtgcga
    68641 tcgttgacgc caaaacagca gaacatgctg ctggtatgac agcaatgaaa acagcaactg
    68701 ataatgctgc taatatcatt gatgagctga cggtttctta caaccgtgca agacaagggg
    68761 ctattaccca agaaattacc gaaattgttg ccggagcttc ggcactagaa tagtattgag
    68821 attggaggaa aaaagatgag ttcaggcaag attgttcaag taatcggtcc cgttgtcgac
    68881 gtggaatttt ctttagacca atccttacca gatatcaaca acgcattagt tgtttataaa
    68941 aatgacgaaa ataaatcaaa agttgttctt gaagcagcgt tagaattagg ggacggagtg
    69001 atccggacca ttgcgatgga atcaacagat ggtttacaaa gaggaatgga agtcattgat
    69061 acaggcaaag caatctctgt tcctgtaggt aaagaaacat taggtcgcgt attcaacgta
    69121 ctaggagata cgatcgattt agaaacacct ttcccagaag atgcggaaag aagcgaaatt
    69181 cataaaaaag caccagcctt tgacgaatta agtacaagta cagaaatttt ggaaacaggg
    69241 atcaaagtta tcgatttgct tgccccatat ttaaaaggtg ggaaagtcgg actattcggt
    69301 ggtgccggtg ttggtaaaac cgtactgatc caagaactga tccataatat cgcccaagaa
    69361 catggtggta tttctgtatt taccggtgta ggtgaacgta ctcgtgaagg taatgacttg
    69421 tattatgaaa tgaaagattc aggagttatc gaaaaaacag ccatggtgtt cggacaaatg
    69481 aacgaaccac caggtgcacg tatgcgtgtt gctttgactg gtttgacgat tgcggaatat
    69541 ttccgtgatg tagaaggtca agatgtactg ttgtttatcg acaacatttt ccgtttcact
    69601 caagctggat ctgaagtatc agccttgtta ggacggatgc cttctgcggt tggttatcaa
    69661 ccaacattgg caacagaaat gggtcaattg caagaacgta tcacatctac gaaaaaaggt
    69721 tcgatcacat cgatcc
//
LOCUS       DACELF010000004        68783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.004, whole genome shotgun sequence.
ACCESSION   DACELF010000004 DACELF010000000
VERSION     DACELF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.004"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            234..476
                     /locus_tag="IW687_000259"
     CDS             234..476
                     /locus_tag="IW687_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0949555.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            473..943
                     /locus_tag="IW687_000260"
     CDS             473..943
                     /locus_tag="IW687_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0949556.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1071..2285
                     /locus_tag="IW687_000261"
     CDS             1071..2285
                     /locus_tag="IW687_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0949557.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2995..3183)
                     /locus_tag="IW687_000262"
     CDS             complement(2995..3183)
                     /locus_tag="IW687_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949558.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3274..3483
                     /locus_tag="IW687_000263"
     CDS             3274..3483
                     /locus_tag="IW687_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0949559.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3595..5208
                     /locus_tag="IW687_000264"
     CDS             3595..5208
                     /locus_tag="IW687_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0949560.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5243..6454
                     /locus_tag="IW687_000265"
                     /pseudo
     CDS             5243..6454
                     /locus_tag="IW687_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6477..7403
                     /gene="glsA"
                     /locus_tag="IW687_000266"
     CDS             6477..7403
                     /gene="glsA"
                     /locus_tag="IW687_000266"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0949561.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7611..9071
                     /locus_tag="IW687_000267"
     CDS             7611..9071
                     /locus_tag="IW687_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949562.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9292..11055
                     /locus_tag="IW687_000268"
     CDS             9292..11055
                     /locus_tag="IW687_000268"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0949563.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11139..11597
                     /locus_tag="IW687_000269"
     CDS             11139..11597
                     /locus_tag="IW687_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0949564.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11706..12389
                     /locus_tag="IW687_000270"
     CDS             11706..12389
                     /locus_tag="IW687_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0949565.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12536..13150
                     /locus_tag="IW687_000271"
     CDS             12536..13150
                     /locus_tag="IW687_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0949566.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13166..13372
                     /locus_tag="IW687_000272"
     CDS             13166..13372
                     /locus_tag="IW687_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949567.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13913..15043
                     /locus_tag="IW687_000273"
     CDS             13913..15043
                     /locus_tag="IW687_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0949568.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15135..15890
                     /locus_tag="IW687_000274"
     CDS             15135..15890
                     /locus_tag="IW687_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0949569.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15948..17987
                     /locus_tag="IW687_000275"
     CDS             15948..17987
                     /locus_tag="IW687_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0949570.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18096..19247)
                     /locus_tag="IW687_000276"
     CDS             complement(18096..19247)
                     /locus_tag="IW687_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0949571.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19401..19931)
                     /locus_tag="IW687_000277"
     CDS             complement(19401..19931)
                     /locus_tag="IW687_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949572.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20030..20380)
                     /locus_tag="IW687_000278"
     CDS             complement(20030..20380)
                     /locus_tag="IW687_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0949573.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20537..22066)
                     /gene="ahpF"
                     /locus_tag="IW687_000279"
     CDS             complement(20537..22066)
                     /gene="ahpF"
                     /locus_tag="IW687_000279"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0949574.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22076..22639)
                     /gene="ahpC"
                     /locus_tag="IW687_000280"
     CDS             complement(22076..22639)
                     /gene="ahpC"
                     /locus_tag="IW687_000280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0949575.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22783..23445)
                     /locus_tag="IW687_000281"
     CDS             complement(22783..23445)
                     /locus_tag="IW687_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0949576.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23629..25275
                     /locus_tag="IW687_000282"
     CDS             23629..25275
                     /locus_tag="IW687_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0949577.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25266..26348
                     /gene="uxuA"
                     /locus_tag="IW687_000283"
     CDS             25266..26348
                     /gene="uxuA"
                     /locus_tag="IW687_000283"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0949578.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26456..28009)
                     /locus_tag="IW687_000284"
                     /pseudo
     CDS             complement(26456..28009)
                     /locus_tag="IW687_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28157..29298
                     /locus_tag="IW687_000285"
                     /pseudo
     CDS             28157..29298
                     /locus_tag="IW687_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29424..30503)
                     /locus_tag="IW687_000286"
     CDS             complement(29424..30503)
                     /locus_tag="IW687_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0949579.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30606..31046)
                     /locus_tag="IW687_000287"
     CDS             complement(30606..31046)
                     /locus_tag="IW687_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0949580.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31103..31513)
                     /locus_tag="IW687_000288"
     CDS             complement(31103..31513)
                     /locus_tag="IW687_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0949581.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31693..32127)
                     /locus_tag="IW687_000289"
     CDS             complement(31693..32127)
                     /locus_tag="IW687_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0949582.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32227..33075)
                     /locus_tag="IW687_000290"
     CDS             complement(32227..33075)
                     /locus_tag="IW687_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0949583.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33464..35230
                     /locus_tag="IW687_000291"
     CDS             33464..35230
                     /locus_tag="IW687_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0949584.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35332..36591
                     /locus_tag="IW687_000292"
     CDS             35332..36591
                     /locus_tag="IW687_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0949585.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36738..38039
                     /locus_tag="IW687_000293"
     CDS             36738..38039
                     /locus_tag="IW687_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0949586.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38042..38893
                     /locus_tag="IW687_000294"
     CDS             38042..38893
                     /locus_tag="IW687_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0949587.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38906..39721
                     /locus_tag="IW687_000295"
     CDS             38906..39721
                     /locus_tag="IW687_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0949588.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39872..41038
                     /locus_tag="IW687_000296"
     CDS             39872..41038
                     /locus_tag="IW687_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0949589.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41268..41915
                     /locus_tag="IW687_000297"
     CDS             41268..41915
                     /locus_tag="IW687_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0949590.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41999..42943
                     /locus_tag="IW687_000298"
     CDS             41999..42943
                     /locus_tag="IW687_000298"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0949591.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKVNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43078..44529
                     /locus_tag="IW687_000299"
     CDS             43078..44529
                     /locus_tag="IW687_000299"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0949592.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            44550..45386
                     /locus_tag="IW687_000300"
     CDS             44550..45386
                     /locus_tag="IW687_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0949593.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45386..46045
                     /locus_tag="IW687_000301"
     CDS             45386..46045
                     /locus_tag="IW687_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0949594.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46171..46860
                     /locus_tag="IW687_000302"
     CDS             46171..46860
                     /locus_tag="IW687_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949595.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46866..47822
                     /locus_tag="IW687_000303"
     CDS             46866..47822
                     /locus_tag="IW687_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0949596.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47866..48378
                     /locus_tag="IW687_000304"
     CDS             47866..48378
                     /locus_tag="IW687_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0949597.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(48634..49182)
                     /locus_tag="IW687_000305"
     CDS             complement(48634..49182)
                     /locus_tag="IW687_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0949598.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49271..50452)
                     /locus_tag="IW687_000306"
     CDS             complement(49271..50452)
                     /locus_tag="IW687_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0949599.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(50688..52196)
                     /locus_tag="IW687_000307"
     CDS             complement(50688..52196)
                     /locus_tag="IW687_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0949600.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52355..52546
                     /locus_tag="IW687_000308"
     CDS             52355..52546
                     /locus_tag="IW687_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949601.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            52749..53666
                     /locus_tag="IW687_000309"
     CDS             52749..53666
                     /locus_tag="IW687_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0949602.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            53659..54732
                     /locus_tag="IW687_000310"
     CDS             53659..54732
                     /locus_tag="IW687_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949603.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54880..55398
                     /locus_tag="IW687_000311"
     CDS             54880..55398
                     /locus_tag="IW687_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949604.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55379..55771
                     /locus_tag="IW687_000312"
     CDS             55379..55771
                     /locus_tag="IW687_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0949605.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55847..56782
                     /locus_tag="IW687_000313"
     CDS             55847..56782
                     /locus_tag="IW687_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0949606.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56915..57694
                     /locus_tag="IW687_000314"
     CDS             56915..57694
                     /locus_tag="IW687_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0949607.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            57687..58004
                     /locus_tag="IW687_000315"
     CDS             57687..58004
                     /locus_tag="IW687_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0949608.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58248..59858
                     /locus_tag="IW687_000316"
     CDS             58248..59858
                     /locus_tag="IW687_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0949609.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60015..60863
                     /locus_tag="IW687_000317"
     CDS             60015..60863
                     /locus_tag="IW687_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0949610.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61094..61573
                     /locus_tag="IW687_000318"
     CDS             61094..61573
                     /locus_tag="IW687_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949611.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            61606..63834
                     /locus_tag="IW687_000319"
     CDS             61606..63834
                     /locus_tag="IW687_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0949612.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            63963..65606
                     /locus_tag="IW687_000320"
     CDS             63963..65606
                     /locus_tag="IW687_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0949613.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(65679..66224)
                     /locus_tag="IW687_000321"
     CDS             complement(65679..66224)
                     /locus_tag="IW687_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0949614.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(66247..66732)
                     /locus_tag="IW687_000322"
     CDS             complement(66247..66732)
                     /locus_tag="IW687_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0949615.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(66722..67534)
                     /locus_tag="IW687_000323"
     CDS             complement(66722..67534)
                     /locus_tag="IW687_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0949616.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(67673..>68783)
                     /locus_tag="IW687_000324"
     CDS             complement(67673..>68783)
                     /locus_tag="IW687_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0949617.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        23019 a        12149 c        13516 g        20099 t
ORIGIN      
        1 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
       61 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      121 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      181 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      241 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      301 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      361 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      421 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      481 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      541 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      601 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      661 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      721 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
      781 taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta catcgtatat
      841 tcctataaaa gatattatcc tacactttga aaaatacgat tatcgttttc attggaatag
      901 tgataattca gttattgaaa tttggatacc tattcaagat tagaagcaat tttatatcca
      961 tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt tttaatgaca
     1021 ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga atgaatgata
     1081 aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca caaacaaggc
     1141 ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa ggaaaacagc
     1201 cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc aatccgcaag
     1261 aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg gacgcattgg
     1321 cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa gattatggaa
     1381 aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt tccatgcagg
     1441 agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg gaatgttatc
     1501 tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg gctggtggtg
     1561 tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat attccaaagt
     1621 taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag aaagattaca
     1681 gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc ttatatctcg
     1741 gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag cagtatgtca
     1801 agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata cgcatgaaga
     1861 acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct gaacgcaccg
     1921 ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac aagccaaaaa
     1981 gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga gagccgatac
     2041 gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg ctacaaagac
     2101 aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt acaacgatac
     2161 tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta ttacaattag
     2221 agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat gacggtattt
     2281 tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca ttccctatgt
     2341 gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc tacttgcgta
     2401 cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca ttaaatcggg
     2461 gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt tgaaaaaatg acggaaattc
     2521 gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat catcattttg tattcaaaca
     2581 ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata ctttgttctc aacttaacaa
     2641 tttaaggttt ccgttcccta tatggtgcaa agaaaatatt tcattctatt gttggcagta
     2701 acggtatggt ttgggtagtt agggtgtgaa aaggaaaata ttcttttttc atcatcaact
     2761 tagcaaaatg acattttcta tccctatatg gtgcatgaaa agaagttaaa atttttccat
     2821 ttaaacttag caaatggacg tttgctgtac agatagtagt gaaaaaggag aaaatcaacc
     2881 gttgattgcc aaaatgaacc gttccatttg tactaataat ggaaaaagtt ttggattttg
     2941 gttacgtttc tcctcttttc caacgcgata tataggaaag acgatttttt tctttcaatc
     3001 gttctttgac aactgaataa agctattcaa tacgtttgac agacagctag ccgttgaaac
     3061 aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa cctatttgtg ttcttgtaaa
     3121 agatttgtcc tcttttatcg ccatgacctg tactgtcaga taatgatact cccgtacagc
     3181 cacagcttga gcattaagag cgtcgcacgc aatgggtacg gctacataag aactatgcag
     3241 aggtggaact cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga
     3301 aagggggtat gtgttattga taatgctgta aaaacaattc aaaagaaaaa tggcaagtgt
     3361 ggcattggca acagaggaaa gacaaagatt gtagtaaaag agcatttttc cgaaaagggc
     3421 aaaacaatgg aagaacttct aactgatgtc atgctggaaa aagcaaagca gacaatcgca
     3481 taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt gttatacttt
     3541 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
     3601 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacagggc
     3661 gaaagttcca gcattaccac acagagaagt atgttgcgtt tatatgcaaa agaacatcat
     3721 ttgaatgtca ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgacagaccg
     3781 agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
     3841 gacctttccc gtcttggcag aaattatatt atgacaggac aatatacaga attgtatttc
     3901 cctagtcata atgtccgcta catagcgatt gatgacggtg tagacagcga aaaaggcgaa
     3961 agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga tacaagccgt
     4021 aaagtcaaat cagcatttaa gacaaagttt gcggaggggg ctcattacgg tgcttatgct
     4081 ccgttaggat ataagaaaca ccctgacatc aaaggaaaac tgttggttga tgatgaaaca
     4141 aaatggatta ttgaaaaaat cttttcccta gcctatcaag gttacggaag tgcaaaaatc
     4201 acaaagcagt taagagcaga aaaagttccg acagcgtcat ggctaaattt tacaagatac
     4261 ggtacttttg cacatatctt tgagggaaaa cccgaaagta agcgttatga gtggacgatt
     4321 gctcatgtca aggcgatatt gaaaagtgag gtttatatcg gaaacagtgt tcacaatatg
     4381 cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta agcccgaaag tgaatggttt
     4441 cgagtagaaa acactcacga gccgattatt gacaaggaag tgttctatcg tgtgcaggag
     4501 cagataaaat caagacgcag acagacaaag gaaaaggcaa cgccgatttt tgcagggctt
     4561 gtcaagtgtg cggattgtgg ttggtctatg agatttggaa cgaataagac aaataaaacg
     4621 ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtaa ttgttctatg
     4681 cactacattc gttatgatgt gctgtatcaa gccgtattgg aacgcttgca gtattgggct
     4741 aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc tggcaatgca
     4801 gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga gaacagacaa
     4861 aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
     4921 gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga actggaacag
     4981 cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat aggtgctgaa
     5041 aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc accattatta
     5101 aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga taacgaaaga
     5161 atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc aacaagagta
     5221 atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     5281 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     5341 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     5401 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     5461 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     5521 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     5581 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     5641 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     5701 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     5761 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     5821 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     5881 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     5941 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     6001 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     6061 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     6121 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     6181 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     6241 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     6301 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     6361 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     6421 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     6481 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     6541 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     6601 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     6661 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     6721 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     6781 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     6841 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     6901 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     6961 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     7021 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     7081 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     7141 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     7201 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     7261 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     7321 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     7381 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     7441 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     7501 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     7561 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     7621 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     7681 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     7741 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     7801 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     7861 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     7921 gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca atcaaaaaaa
     7981 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     8041 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     8101 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     8161 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga gaaatagaga
     8221 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     8281 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     8341 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     8401 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     8461 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     8521 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     8581 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     8641 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     8701 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     8761 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     8821 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     8881 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     8941 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     9001 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     9061 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     9121 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     9181 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     9241 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     9301 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     9361 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     9421 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     9481 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     9541 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     9601 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     9661 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     9721 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
     9781 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
     9841 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
     9901 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
     9961 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
    10021 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
    10081 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
    10141 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
    10201 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
    10261 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
    10321 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
    10381 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
    10441 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
    10501 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
    10561 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
    10621 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
    10681 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
    10741 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
    10801 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
    10861 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
    10921 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
    10981 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
    11041 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
    11101 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
    11161 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
    11221 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
    11281 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
    11341 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
    11401 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
    11461 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
    11521 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
    11581 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
    11641 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
    11701 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
    11761 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
    11821 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
    11881 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
    11941 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
    12001 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
    12061 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
    12121 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
    12181 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
    12241 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
    12301 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
    12361 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
    12421 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
    12481 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
    12541 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
    12601 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
    12661 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
    12721 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
    12781 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
    12841 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
    12901 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
    12961 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
    13021 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
    13081 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
    13141 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
    13201 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
    13261 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
    13321 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
    13381 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
    13441 tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa
    13501 aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta
    13561 ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc
    13621 ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa
    13681 ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta
    13741 aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt
    13801 ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag
    13861 tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ttttgggtcc
    13921 ttatttgact ttgtccatga tcggtctttt agaagtatac agtgccagtt cttatcgttt
    13981 attgcaagcc gatgaaaata caaaaagtct attattgcgt caactaattt tcatattttt
    14041 gagttggagc gtgatcttct tagctcgttc agtcaaacta cactatttac ttcaccctaa
    14101 aatagcagga tacggtttag ccttatcgat tttcttttta gtattagtaa gaatagggat
    14161 attcggtgtc actgtcaacg gcgcacaacg ttggatctct ctgtttggca ttcaattcca
    14221 gccttctgaa ctggcaaatc tttttttgat tttttattta agctggtttt ttcgtgacgg
    14281 aaatagtagc ccaaaagatc taaaaaaacc attcctgatt acagtaggta taacttttct
    14341 gattttattt cagccaaaga ttgctggagc attgatgatc ctttcgattg cgtgggtcat
    14401 attttgggca gcggcggttc catttaaaaa agggatctat ctaatcgtta ctttttctgc
    14461 attgctgatt ggagcagcag gcggggtatt atatttagga aataaaggtt ggcttccaca
    14521 gatgtttaat catgcttatg aaaggatcgc gactttaaga gattctttta tcgatagtca
    14581 tggtgcgggc tatcaaatga cccattcgtt ttatgcacta tataatggtg gcatatttgg
    14641 gcggggatta ggaaatagta tcacaaaaaa agggtatctt ccagagacag aaacagattt
    14701 tattttttcg attattacag aagagcttgg gctaatcggt gcattgtgtg tgcttttctt
    14761 attgttctca ttatgcatga gaatcttttg tttaagcagt cgctgtaaaa atcagcaagc
    14821 tggattgttt cttttaggat tcggaacttt gctattcgtt cagacgataa tgaacgtggg
    14881 aagtatcgcg gggctgatgc caatgactgg tgtgccattg ccatttgtta gctacggagg
    14941 aacaagttat ctgatccttt ctttaggaat cggaatcacg ttgaatattt cttcaaagat
    15001 acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa taaagagaaa ctgagaaact
    15061 ctcagtttga attatcataa agtacaatct tttatttttc aagcgagagg ttcttttaga
    15121 aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt atctattttg ttactgctta
    15181 cattagtagt tggctatttt tcgattgagt ttatccatgg attttcgtct gcaaaacaga
    15241 gttcaactgt gaaaaaggta gagccgaaaa gtatccctac cacactaaat gtggctttga
    15301 ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc ggattcactt ctggttgcac
    15361 aatacaacca gaaaacacaa caagcaaaac tgatctctat catgagagat tcatatgtcg
    15421 atattccagg ttacggaatg aataagataa atgcagctta ctcttatggg ggagttgatt
    15481 tattaaatca aacattaaag gaaaatttca aatttgaagc tccctattat gcaagtatca
    15541 catttcaaga ttttatcgat tgcgttaatg aactgtttcc tggtggagtc aagatcgatg
    15601 cagaaaaatc tctagattta gatggcgtat atataaacaa aggagagcaa gtgatggatg
    15661 gtaatacttt actccagtat gctcgatttc gggaagacaa agaaggagac ttcgggagga
    15721 tcagaaggca gcagcaagtg atcaaagcta tctctcagca gctgaaagac gtaacttcaa
    15781 tattcaagct acccaaagcg gttgggaaat tacttggtag tatccaaacg aaccttccag
    15841 aaagtgtgct tctggattgt ggtatggatt tcctcaaaga catatgttag aataaacagg
    15901 tataaatagt gaaataaagg aatgacaagc aagagaagga ggaaaaaatg aaaagaagtg
    15961 acaagcacgg caaaaatcga acaggcgctt atattgccgg cgcagtgatc ttaatagcag
    16021 ctgcgggtgg cggttatttt tactaccagc actatcaaga aacccaagca gtagaagctg
    16081 gagaaaagac agttgagcaa tttgtccaag ctttgaacaa gggagattat aacaaagctg
    16141 cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt atctgaaaaa gagatcttag
    16201 ataaatacca gaatatatac ggtgctgctg atgtcaaagg acttcagata tcgaatctaa
    16261 aagtagataa aaaagacgat tctacttaca gtttttcata taaagcaaag atgaatacct
    16321 cattaggtga attgaaagat ctttcttata aaggaacatt agacagaaat gatggtcaaa
    16381 ccacgatcaa ctggcaaccc aacttggttt ttccagaaat ggaaggaaat gacaaagtaa
    16441 gcctgaccac acaagaagca gcaagaggga atattataga tcgaaatggt gaaccattag
    16501 caacaacagg caaactaaaa caattaggag tcgttccaag taaacttgga gatggaggcg
    16561 aaaaaacagc caatatcaaa gcgattgctt cctccttcga cttaacagaa gatgctatca
    16621 atcaggcgat ttcacaaagc tgggtacaac ccgattactt tgtcccattg aaaatcattg
    16681 atggagcaac gccagaactt ccagctggag ctaccattca agaagtagac ggcagatatt
    16741 atcctttggg tgaagcagct gctcagctga ttggttacgt gggagatatc acagcagaag
    16801 atattgataa gaatccagaa ttaagcagta atggaaaaat cggtcgctca ggtttggaga
    16861 tggcttttga taaggatctt cgtgggacaa caggtgggaa attaagcatt acagatgcag
    16921 acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca aaatggcaaa gatatcaaat
    16981 tgacaatcga tgcgaaggca caaaaaacag cttttgacag tctaggagga aaagctggat
    17041 caactgttgc gacaacgcca aaaaccggtg atcttcttgc gcttgctagc tctccaagct
    17101 atgatccaaa caaaatgaca aacgggatct cacaagaaga ttacaaagct tatgaagaaa
    17161 atcctgaaca tccattcatc agccgatttg cgacaggtta tgctcctgga tctacgttta
    17221 aaatgattac agcagcaatc ggtctcgaca acggcactat cgatccaaat gaagtgttga
    17281 cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg atcttatcaa gcaacgcgtg
    17341 tcagtagtga tgtatcacaa gtagacttaa aaactgcttt gatctattcc gataatatat
    17401 atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt tcgtacaggc ttagataaat
    17461 tcatttttgg tgaagacctt gatttgccaa ttagtatgaa tccagcacaa atttctaatg
    17521 aagatagctt taactcagat atcttgctag ctgatactgg atatggacag ggcgaacttc
    17581 taattactcc tatccagcaa gcagcaatgt attctgtttt taccaacaat ggcacacttg
    17641 tctatcctaa attgattgca gataaagaga caaaagataa gaagaatgtc atcagcgaaa
    17701 cagcagtaca aacgatcgtg ccagatctga gagaagttgt gcaagatgta aatggtacag
    17761 cacattctct ttctgcttta gggattccgt tggcagcgaa aactggtaca gcggaaatca
    17821 aagaaaaaca ggatgtaaag gggcaagaga acagtttctt gtttgctttc aatcttgata
    17881 accaaggtta tatgatggtc agcatgttgg aaaataaaga agatgatgat tcagcaacca
    17941 aacgagcatc ggaactattg caatacctca accaaaatta tcaataaaaa attgcttttt
    18001 aagtacagta aaaggactgt ggcgttatcg tcacagtcct tttttcaaat aaacggtttt
    18061 cacgatgtct ctttcacaac ttctttttga tttactcaca cttcataaac tttttttgta
    18121 aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat aactaaaggt
    18181 gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat gatcaaagcc
    18241 atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg agaaaaaccg
    18301 gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac aagcgtcaat
    18361 atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa tcctacactg
    18421 acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg gtatacttct
    18481 tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat gacagagcta
    18541 agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga tacgatgata
    18601 atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc aatccatttg
    18661 actaataaga aaataaaaac aaaataaatt agctgttcaa tcaaaacaag cggtaaagaa
    18721 gcattgctag aagcttcccc cgttagaaaa gataggctga aacttagaac taaaacgact
    18781 aaaatgtcat ctaccacaga agcacccaaa attgttgatc cttcttttgt attcactacg
    18841 tttagttctt ttaatacttc tactgaaata ctgacagaag ttgccgcaag aataatccca
    18901 aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc ctgtcaacca gccaaaaaca
    18961 accgggaaga tgatcccaag caaagcaacg aacataccag gtcggaaata ttttttcaaa
    19021 agagataaat cactttccag accagctaaa aacattaata agatcacacc aatctccgaa
    19081 aagtcatgga ctagaatatc aggtttgacc caattcaatc cagcttggcc tagcaaaaca
    19141 ccaacaagta attgtccaat cactgctggg atacctaatc tccgcgagat atgtgagcca
    19201 atcgttgttg aaatcaatat caggcataaa ataccaataa attccattct catcattcct
    19261 ttgtataaaa tatagcccga acttagcata acatacatta ttgaaaaaat aggaaaatat
    19321 actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt tatacggtct
    19381 attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat gaaattgata
    19441 atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag aagcatcaaa
    19501 cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat actactataa
    19561 acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa gctgttgatg
    19621 ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc aaaagcatag
    19681 acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact ccaagaacct
    19741 gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa aacaagatag
    19801 gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac aatcgcaatc
    19861 ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt ttgtttaatc
    19921 gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag tcattttacc
    19981 aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat caacttctga
    20041 ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct aaataacgaa
    20101 tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca acacctgtaa
    20161 tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg aaagtcactt
    20221 gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa caaatgattt
    20281 catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac ataggataaa
    20341 cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac ctttcttttt
    20401 ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg attattcaaa
    20461 aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct ctctgacttt
    20521 ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag
    20581 ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa tcaccagctg
    20641 cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca tttcttggat
    20701 tcatttcgat cgttccatct agccaatccg tattagggag taagccgact agaatgaata
    20761 ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag gagagagatt
    20821 ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca gtgacattcg
    20881 caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt aagaattcta
    20941 gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca ccagaattac
    21001 caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag tgtgggcaat
    21061 aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa cgccagtgcg
    21121 cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct aagccaatat
    21181 taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata atgtcgatag
    21241 ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct gtgtaagagg
    21301 taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa gaatcagcga
    21361 caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta gctgctggtc
    21421 caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct tcaatcaaag
    21481 ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta aattcttctc
    21541 catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct ttttggaaca
    21601 tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat atattcaatg
    21661 cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca aaatgcaatg
    21721 tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt gcccgtccac
    21781 taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg ataccagcaa
    21841 aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg cgcatcgaat
    21901 ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta aattcagcta
    21961 gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga gattcaatca
    22021 gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc accacctaaa
    22081 tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc cattttgccg
    22141 gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta tcaatcagtg
    22201 cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg attccgtctg
    22261 gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa acatcaaaaa
    22321 tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta ccgatagcat
    22381 ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt
    22441 caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat tcagtaggac
    22501 aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc ataagatctt
    22561 tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat tcttcgattt
    22621 ttttcccaat caatgacata gtaaaacctc caataataat tatttattta taattattgt
    22681 aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga ttactcaaaa
    22741 acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct ggaaatactc
    22801 gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt
    22861 ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat cataatgttg
    22921 ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc ggaaacgttc
    22981 taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg caatttcgaa
    23041 acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac
    23101 tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt
    23161 ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc gagcattttc
    23221 tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg tatacacgag
    23281 ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga tagcaagatg
    23341 ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat agataatttg
    23401 tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta ttcctccaaa
    23461 atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat
    23521 taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata
    23581 aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta
    23641 aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt
    23701 gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc
    23761 ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa
    23821 ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac
    23881 caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa
    23941 cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt
    24001 gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt tgctattact
    24061 gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat tgcagtcgat
    24121 ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgt gctactttat
    24181 aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa tttttcacaa
    24241 aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca aaaggaaggt
    24301 tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc gtttccatgg
    24361 acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa
    24421 gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat tgctccattt
    24481 gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa tggacgtccg
    24541 aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa agcagatacg
    24601 atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat gggttgttta
    24661 ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct gctagtttct
    24721 gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat tgatccaaag
    24781 atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc taatatgcct
    24841 cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga gacgattaaa
    24901 aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc attgactctc
    24961 gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt catcccgagt
    25021 ccagacccac tattgacaga ccttcagcat aagctttccg atattcaact gggtacgcaa
    25081 gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga aatttttggt
    25141 gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat caagatggta
    25201 gcaggaccaa atgccgtacg aaaaacatta caggattact tacaagaaca tggaggaaat
    25261 ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt accttagatg
    25321 aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat ataccagtag
    25381 gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag tctcgtgggt
    25441 taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt ggattacctt
    25501 cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca gaatatggaa
    25561 tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct gaccttgact
    25621 accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt ccagatgatc
    25681 caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttggattg ccgggatggg
    25741 aacctgagcg cttagctcct atcaaatcct tgcttgaagc ctatgcatca gtagacgaag
    25801 aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca tgtgaagagg
    25861 taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt ggattagctc
    25921 gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg catagtagac agcccttata
    25981 acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat gtatatggga
    26041 ttttagcaga attttgccaa agagatcgaa tcccatttgc acatgttcga aatattaaat
    26101 ttttagctaa tcgagatttc tatgaggcac cacataaatc cgaatacggt tcattagaca
    26161 tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc cgtcctgatc
    26221 atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat gatcgagcat
    26281 taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac aaaagtaaag
    26341 ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt caaattcttg
    26401 atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat ttggtctagg
    26461 aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt aattcaaaga
    26521 tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg attcctttta
    26581 aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg ctgataacga
    26641 tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa aaaatttgtt
    26701 ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc acaccaatca
    26761 caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca accatccatt
    26821 gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct ctgaaatcca
    26881 tataaaattg ttcaatggcg gtttggactt ctttcaatac gctatgttct tgataaagca
    26941 tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta taaagtttat
    27001 ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac aaatcaaata
    27061 cagtacaaag ggactccacc atttctcttg aataaagaac atgaggaacg tttgcttttt
    27121 ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg atagaaagtc
    27181 gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa
    27241 tatctacaga gagatcaata aaaaagtcaa tatattcata ctctgtgaat tctttttgta
    27301 cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga aaatataatt
    27361 tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca
    27421 taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt gaaaagatgt
    27481 tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa aaagactttc
    27541 tgccattatt agaaaaggag atagacaaac cgtactatgc taaaaatgca ttaatctttt
    27601 tcattttcgt atacaaataa gaggcactat agtttgtttg ctgactgaaa ctgataatcg
    27661 aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat tcgttgcagt
    27721 atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa ttcaacttta
    27781 aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca aagtcttgtt
    27841 ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga tctgctaaat
    27901 attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct aattttgtat
    27961 tgcttttttt cttcaagata aacgttttaa tcgcttcttg agtttgcatg aaagaggctc
    28021 ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa atttagacaa
    28081 aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata caaaaaaatc
    28141 aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg cagccttaag
    28201 cttatcgctt actataaatc agacagtcca cacagaggag acagaaacag ccaaatggac
    28261 agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt catccttcaa
    28321 ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa aaattgacta
    28381 ttcgggagac ttagcaccta tactacaatc agctgttaca tttgagaatg ttgatcccac
    28441 tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag cgatatttac
    28501 cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg tcaaggtatc
    28561 agatggacta ccgaacgatc atcatttaac gccgtatagt cagcggcaga caattgagca
    28621 agattcagcc atcgactcag gaggagatct tgttgaagaa ccaacagata aaccagaaaa
    28681 tgaaaataag ccagaagtac cgcctacaga aaatccagac ggagaacaga aaccggaaat
    28741 agaacctgga gaagaacccg atacagaaac tcaaccagag ccagacaata aatcaaaacc
    28801 tgaaataacc cctggagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa
    28861 ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc
    28921 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
    28981 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
    29041 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
    29101 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
    29161 tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata
    29221 ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa
    29281 caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga
    29341 aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata
    29401 ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg
    29461 atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag
    29521 ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc
    29581 agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca
    29641 atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg
    29701 aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa
    29761 atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg
    29821 agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg
    29881 acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa
    29941 aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg
    30001 aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc
    30061 gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat
    30121 tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc
    30181 atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg
    30241 taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc
    30301 tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt
    30361 gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg
    30421 cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa
    30481 caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag
    30541 aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag
    30601 cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt
    30661 tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata
    30721 ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt
    30781 tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag
    30841 ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt
    30901 ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg
    30961 aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact
    31021 tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca
    31081 tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc
    31141 cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt
    31201 cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt
    31261 cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt
    31321 gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt
    31381 gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg
    31441 tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat
    31501 acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa
    31561 tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa
    31621 acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc
    31681 tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc
    31741 acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt
    31801 cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc
    31861 tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg
    31921 ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt
    31981 accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag
    32041 ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac
    32101 aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta
    32161 ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt
    32221 accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct
    32281 gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc
    32341 atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc
    32401 ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa
    32461 ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt
    32521 catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa
    32581 aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt
    32641 caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt
    32701 gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa
    32761 caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa
    32821 gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa
    32881 cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag
    32941 taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt
    33001 gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg
    33061 tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta tcttattata
    33121 acttactttt caccttaagt taactttaat taataaaaaa attaggctag tctaaattat
    33181 tatgtttcat tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa cttttacttt
    33241 agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt
    33301 agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt
    33361 cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc
    33421 acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat
    33481 ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg
    33541 gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac
    33601 cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa
    33661 tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt
    33721 ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta
    33781 tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat
    33841 cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga
    33901 aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa
    33961 agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga
    34021 ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc
    34081 caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa
    34141 acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc
    34201 tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga
    34261 aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat
    34321 gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt
    34381 catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac
    34441 ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga
    34501 tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat
    34561 ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt
    34621 gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc
    34681 ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga
    34741 aggaacattc aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg
    34801 taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg
    34861 tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg
    34921 tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt
    34981 ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt
    35041 aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt
    35101 ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag
    35161 tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat
    35221 catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc
    35281 ccattataat aagggtgtaa cagcacgtag aatacaaagg aggaaatgaa gatgaaaaaa
    35341 agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg
    35401 gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca
    35461 gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa
    35521 gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta
    35581 gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc
    35641 ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac
    35701 gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta
    35761 atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt
    35821 aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa
    35881 aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt
    35941 tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat
    36001 gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag
    36061 ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa
    36121 actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat
    36181 tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga
    36241 ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt
    36301 gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct
    36361 gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg
    36421 attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg
    36481 acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc
    36541 gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga
    36601 taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag
    36661 atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt
    36721 ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca
    36781 cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta
    36841 tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg
    36901 cattggtagg attggttact ttgggaagta taccaatgga agatcattca ttatttattt
    36961 tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga
    37021 atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga
    37081 cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc
    37141 ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg
    37201 catttactaa ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg
    37261 ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct
    37321 tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc
    37381 tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt
    37441 tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca
    37501 acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg
    37561 tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct
    37621 tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat
    37681 cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct
    37741 tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt
    37801 atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa
    37861 gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga
    37921 ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg
    37981 ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac
    38041 aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta
    38101 cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac
    38161 tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt
    38221 aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt
    38281 gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac
    38341 ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca
    38401 gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct tattagaagc
    38461 gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac
    38521 ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact
    38581 tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat
    38641 gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa
    38701 gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc
    38761 tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc
    38821 gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg
    38881 aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt
    38941 acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt
    39001 gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca
    39061 gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg
    39121 aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac
    39181 gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga
    39241 tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc
    39301 tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac
    39361 gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat
    39421 cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt
    39481 attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac
    39541 attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc
    39601 agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac
    39661 tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg
    39721 acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt
    39781 tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg
    39841 taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt
    39901 attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca
    39961 atacggagat acggacactc ctacccaaat caaaatcaat atccatgaag aaatcccaat
    40021 gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg atcatcgaat
    40081 cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt
    40141 tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa
    40201 aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata aactttggct
    40261 agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca
    40321 agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac
    40381 gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa
    40441 tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat
    40501 aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa
    40561 aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa
    40621 tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat
    40681 ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt
    40741 ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt
    40801 gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac
    40861 aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg
    40921 aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt
    40981 gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt
    41041 gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga
    41101 agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat
    41161 attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg
    41221 ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg
    41281 ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc
    41341 aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact
    41401 cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta
    41461 ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc
    41521 cagatactgc aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga
    41581 tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa
    41641 tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag
    41701 aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg
    41761 acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt
    41821 attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa
    41881 caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg
    41941 atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt
    42001 gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg
    42061 tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt
    42121 tatcactaag agctgtacga tcaatgaacg aaaagtaaat cccgaaccac gctattttga
    42181 tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt cttattatct
    42241 agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc
    42301 cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg
    42361 gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact
    42421 agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa
    42481 gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga
    42541 tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt
    42601 gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat
    42661 tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt
    42721 gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga
    42781 acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc
    42841 ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac
    42901 gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca
    42961 tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta
    43021 ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg
    43081 aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta
    43141 gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg
    43201 cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca
    43261 ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct
    43321 acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg
    43381 caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc
    43441 aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt
    43501 acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta
    43561 gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt
    43621 gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa
    43681 ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat
    43741 gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca
    43801 catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag
    43861 attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg
    43921 catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat
    43981 cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga
    44041 gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag
    44101 ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg
    44161 gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct
    44221 aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat
    44281 tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc
    44341 acaccttata cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat
    44401 ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat
    44461 ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca
    44521 tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa
    44581 ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca
    44641 gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc
    44701 ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag
    44761 aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa
    44821 tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag
    44881 aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg
    44941 tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag
    45001 ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca
    45061 catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg
    45121 ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg
    45181 taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag
    45241 gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga
    45301 cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa
    45361 cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg
    45421 gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc
    45481 gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt tggattggct
    45541 gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt
    45601 cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa cggcttcagt
    45661 catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc
    45721 aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt
    45781 attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt
    45841 agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt
    45901 tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct atattttagt
    45961 tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa
    46021 acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt
    46081 tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt
    46141 aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa
    46201 tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg
    46261 cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga
    46321 gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg
    46381 tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa
    46441 actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag
    46501 atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta
    46561 ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca
    46621 aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa agaattgaaa
    46681 agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag
    46741 gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat
    46801 tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga
    46861 aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag
    46921 cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt
    46981 ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcga atcgctgtga
    47041 tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag
    47101 tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag
    47161 tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag
    47221 aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct
    47281 ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc
    47341 atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc
    47401 acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata
    47461 aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata
    47521 tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt
    47581 ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga
    47641 ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg
    47701 gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc
    47761 atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat
    47821 gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa
    47881 acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt
    47941 gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg
    48001 aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca
    48061 gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa
    48121 tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa
    48181 aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa
    48241 gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct
    48301 ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat
    48361 caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact
    48421 gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac
    48481 aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa
    48541 atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct
    48601 ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt tttttatttt
    48661 atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt
    48721 caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc
    48781 agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa
    48841 gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa
    48901 aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata
    48961 tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa
    49021 tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg
    49081 attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt
    49141 tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat
    49201 gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct
    49261 tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg
    49321 ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc
    49381 ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga
    49441 cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca
    49501 tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt
    49561 ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt
    49621 ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa
    49681 aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc
    49741 ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt
    49801 cttctaatac atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa
    49861 caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca
    49921 tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg
    49981 gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc
    50041 cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt
    50101 tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata
    50161 caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa
    50221 aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata
    50281 cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta
    50341 aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa
    50401 aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac
    50461 tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg
    50521 aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa
    50581 caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat
    50641 cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat
    50701 agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt
    50761 tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct
    50821 agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc
    50881 aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct
    50941 tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa
    51001 tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg
    51061 gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga
    51121 ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca
    51181 ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc
    51241 aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag
    51301 gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca
    51361 gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca
    51421 ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg
    51481 aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc
    51541 agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct
    51601 gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag ctttccactt
    51661 cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt
    51721 ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag
    51781 tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc
    51841 tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca
    51901 tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta
    51961 cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc
    52021 agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg
    52081 tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa
    52141 atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg
    52201 ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag
    52261 cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca
    52321 ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa
    52381 cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc
    52441 cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga
    52501 gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag
    52561 gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg
    52621 taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt
    52681 tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg
    52741 caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga
    52801 cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag
    52861 caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg
    52921 aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa
    52981 gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt
    53041 ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt
    53101 acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag
    53161 aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa
    53221 cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct cagacagaaa
    53281 ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca
    53341 ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga
    53401 agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg cctgaaacaa
    53461 tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt cgtgcagcca
    53521 tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag ggattcggtc
    53581 aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat aatcaatcta
    53641 atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa aaatgaagaa
    53701 caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag taatcctctg
    53761 gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta
    53821 actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac tgaaaatccc
    53881 gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat tgaaacagcg
    53941 gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga ttttccacaa
    54001 ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac gatcggaaag
    54061 acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga tattggtctg
    54121 gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat caattatacg
    54181 ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga tcaacgatac
    54241 caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat caatgtccaa
    54301 gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt ctctgcaggg
    54361 cagatcacga tattcaatca caaattgaat caattattag cccatgcaag aacacttggt
    54421 tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa tcttcagagc
    54481 gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat gaaaaatcca
    54541 tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga taaaggacag
    54601 tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc atctggagac
    54661 tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc attaatggtg
    54721 agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt ctgttccaaa
    54781 ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt actaaatgga
    54841 cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa taaaatagca
    54901 cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca atttattccg
    54961 aatttccagc caatgactgc tattttttta tttatagtac tttacttaag cttaaaagat
    55021 gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg tgttggacca
    55081 tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc aatcgtctgc
    55141 ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt tctggcgggc
    55201 atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt gcctcaacct
    55261 tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg caatgttgtg
    55321 ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat
    55381 gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt tgacaagttg
    55441 cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa
    55501 agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa
    55561 aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag aagataacgg
    55621 cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct attggatgtt
    55681 tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga atgatggaca
    55741 atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta aagggcgaat
    55801 attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg aaaattttaa
    55861 aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag ttgctatcag
    55921 caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa gcatccacag
    55981 tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag ccaataaagg
    56041 acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca gtacaatcgt
    56101 cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt ttgaaaagtt
    56161 gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac tgttatttgg
    56221 tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac
    56281 tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa tactgattat
    56341 ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag gaacaatatt
    56401 gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga tcggtttctt
    56461 gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca tcggtgaaag
    56521 cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg agatcagtcg
    56581 gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg cagcaaaagc
    56641 agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa
    56701 tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag ataaatatga
    56761 cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat
    56821 ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg
    56881 ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg
    56941 gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc
    57001 ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat
    57061 tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc
    57121 atttgcaacg acattattga tggttttctt tttagaatta cgctatacat tattaggagc
    57181 aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc
    57241 actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa
    57301 taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat
    57361 gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt
    57421 gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac
    57481 aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg
    57541 attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa
    57601 aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa
    57661 cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt
    57721 gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa
    57781 aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt
    57841 tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa
    57901 tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag
    57961 ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt
    58021 aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca
    58081 gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg
    58141 caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat
    58201 aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat
    58261 tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag
    58321 tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga
    58381 cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat
    58441 ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt
    58501 gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg
    58561 cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata
    58621 atgggatact agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg
    58681 caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta
    58741 tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga
    58801 ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt
    58861 tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag
    58921 attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca
    58981 ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg
    59041 accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac
    59101 tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct
    59161 ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag
    59221 atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc
    59281 ttccttcatt gactggaatg tctttattag ctggaccact atacacattg ttttttggct
    59341 atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt
    59401 tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat
    59461 taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg
    59521 gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct
    59581 atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct
    59641 taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc
    59701 tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag
    59761 gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt
    59821 tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg
    59881 tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta
    59941 gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg
    60001 aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac
    60061 gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa
    60121 tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta
    60181 aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat
    60241 tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct
    60301 ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg
    60361 actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg
    60421 atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct
    60481 acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg
    60541 gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga
    60601 aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa
    60661 ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa
    60721 ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag
    60781 aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg
    60841 gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatattccaa
    60901 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
    60961 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
    61021 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
    61081 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
    61141 agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact
    61201 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
    61261 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
    61321 agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
    61381 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
    61441 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
    61501 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
    61561 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
    61621 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
    61681 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
    61741 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
    61801 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
    61861 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
    61921 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
    61981 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
    62041 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
    62101 gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc
    62161 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
    62221 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
    62281 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
    62341 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
    62401 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
    62461 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
    62521 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
    62581 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
    62641 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
    62701 ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
    62761 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
    62821 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
    62881 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
    62941 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
    63001 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
    63061 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
    63121 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
    63181 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
    63241 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
    63301 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
    63361 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
    63421 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
    63481 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
    63541 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
    63601 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
    63661 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
    63721 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
    63781 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
    63841 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
    63901 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
    63961 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
    64021 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
    64081 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
    64141 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
    64201 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
    64261 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
    64321 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
    64381 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
    64441 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
    64501 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
    64561 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
    64621 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
    64681 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
    64741 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
    64801 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg
    64861 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
    64921 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
    64981 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
    65041 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
    65101 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
    65161 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
    65221 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
    65281 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
    65341 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
    65401 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
    65461 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
    65521 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
    65581 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
    65641 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
    65701 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
    65761 cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata
    65821 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
    65881 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
    65941 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
    66001 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
    66061 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
    66121 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
    66181 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
    66241 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
    66301 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
    66361 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
    66421 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
    66481 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
    66541 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
    66601 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
    66661 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
    66721 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
    66781 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
    66841 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
    66901 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
    66961 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
    67021 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
    67081 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
    67141 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
    67201 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
    67261 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
    67321 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
    67381 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
    67441 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
    67501 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
    67561 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
    67621 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
    67681 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
    67741 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
    67801 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
    67861 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
    67921 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
    67981 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
    68041 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
    68101 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
    68161 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
    68221 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
    68281 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
    68341 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
    68401 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
    68461 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
    68521 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
    68581 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
    68641 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
    68701 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
    68761 gttccattaa ataaagaata gga
//
LOCUS       DACELF010000005        56286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.005, whole genome shotgun sequence.
ACCESSION   DACELF010000005 DACELF010000000
VERSION     DACELF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.005"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..189
                     /locus_tag="IW687_000325"
     CDS             <1..189
                     /locus_tag="IW687_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0949618.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IW687_000326"
     CDS             complement(1055..1585)
                     /locus_tag="IW687_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ0949619.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IW687_000327"
     CDS             complement(1582..2199)
                     /locus_tag="IW687_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0949620.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IW687_000328"
     CDS             complement(2781..3002)
                     /locus_tag="IW687_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ0949621.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IW687_000329"
     CDS             complement(3180..4712)
                     /locus_tag="IW687_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0949622.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IW687_000330"
     CDS             complement(4848..5933)
                     /locus_tag="IW687_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0949623.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IW687_000331"
     CDS             complement(5948..6667)
                     /locus_tag="IW687_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0949624.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IW687_000332"
     CDS             complement(6664..7974)
                     /locus_tag="IW687_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0949625.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IW687_000333"
     CDS             complement(8001..8591)
                     /locus_tag="IW687_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ0949626.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IW687_000334"
     CDS             complement(8909..9970)
                     /locus_tag="IW687_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0949627.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IW687_000335"
     CDS             complement(10538..11605)
                     /locus_tag="IW687_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0949628.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IW687_000336"
     CDS             complement(11625..12104)
                     /locus_tag="IW687_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ0949629.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IW687_000337"
     CDS             complement(12121..12582)
                     /locus_tag="IW687_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0949630.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IW687_000338"
     CDS             complement(12596..13846)
                     /locus_tag="IW687_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0949631.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IW687_000339"
     CDS             complement(14224..15675)
                     /locus_tag="IW687_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0949632.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IW687_000340"
     CDS             complement(15996..16760)
                     /locus_tag="IW687_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0949633.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IW687_000341"
     CDS             complement(16788..17486)
                     /locus_tag="IW687_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0949634.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IW687_000342"
     CDS             complement(17498..18277)
                     /locus_tag="IW687_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0949635.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IW687_000343"
     CDS             complement(18293..19237)
                     /locus_tag="IW687_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0949636.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IW687_000344"
     CDS             complement(19283..20062)
                     /locus_tag="IW687_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949637.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IW687_000345"
     CDS             complement(20442..22517)
                     /locus_tag="IW687_000345"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0949638.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IW687_000346"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IW687_000346"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0949639.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IW687_000347"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IW687_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0949640.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IW687_000348"
     CDS             complement(24783..25682)
                     /locus_tag="IW687_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0949641.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IW687_000349"
     CDS             complement(25697..26098)
                     /locus_tag="IW687_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0949642.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IW687_000350"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IW687_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0949643.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IW687_000351"
     CDS             complement(26581..28773)
                     /locus_tag="IW687_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0949644.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IW687_000352"
     CDS             complement(28797..29768)
                     /locus_tag="IW687_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0949645.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IW687_000353"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IW687_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0949646.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32008..32454)
                     /locus_tag="IW687_000354"
     CDS             complement(32008..32454)
                     /locus_tag="IW687_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0949647.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32481..32657)
                     /locus_tag="IW687_000355"
     CDS             complement(32481..32657)
                     /locus_tag="IW687_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0949648.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32819..33247)
                     /locus_tag="IW687_000356"
     CDS             complement(32819..33247)
                     /locus_tag="IW687_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0949649.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33339..34199
                     /locus_tag="IW687_000357"
     CDS             33339..34199
                     /locus_tag="IW687_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0949650.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34293..34586)
                     /locus_tag="IW687_000358"
     CDS             complement(34293..34586)
                     /locus_tag="IW687_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0949651.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34659..36386)
                     /locus_tag="IW687_000359"
     CDS             complement(34659..36386)
                     /locus_tag="IW687_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0949652.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IW687_000360"
     CDS             complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IW687_000360"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0949653.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IW687_000361"
     CDS             complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IW687_000361"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0949654.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39103..40506)
                     /locus_tag="IW687_000362"
     CDS             complement(39103..40506)
                     /locus_tag="IW687_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0949655.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40736..41638
                     /locus_tag="IW687_000363"
     CDS             40736..41638
                     /locus_tag="IW687_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0949656.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41679..43565)
                     /locus_tag="IW687_000364"
     CDS             complement(41679..43565)
                     /locus_tag="IW687_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0949657.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43662..44514)
                     /locus_tag="IW687_000365"
                     /pseudo
     CDS             complement(43662..44514)
                     /locus_tag="IW687_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44849..46633)
                     /locus_tag="IW687_000366"
     CDS             complement(44849..46633)
                     /locus_tag="IW687_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949658.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46633..48384)
                     /locus_tag="IW687_000367"
     CDS             complement(46633..48384)
                     /locus_tag="IW687_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0949659.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48526..48750)
                     /locus_tag="IW687_000368"
     CDS             complement(48526..48750)
                     /locus_tag="IW687_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0949660.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49001..50587
                     /locus_tag="IW687_000369"
     CDS             49001..50587
                     /locus_tag="IW687_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0949661.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50653..52428)
                     /locus_tag="IW687_000370"
     CDS             complement(50653..52428)
                     /locus_tag="IW687_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0949662.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52639..53541)
                     /locus_tag="IW687_000371"
     CDS             complement(52639..53541)
                     /locus_tag="IW687_000371"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0949663.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53820..56165
                     /locus_tag="IW687_000372"
     CDS             53820..56165
                     /locus_tag="IW687_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0949664.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16679 a        11714 c         9115 g        18778 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt
    32041 tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc
    32101 aaattctttc attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat
    32161 ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac
    32221 tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg
    32281 tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt
    32341 aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc
    32401 tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc
    32461 aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc
    32521 ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc
    32581 gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga
    32641 acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt
    32701 attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg
    32761 accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt
    32821 attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac
    32881 cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca
    32941 ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact
    33001 tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat
    33061 ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt
    33121 ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt
    33181 tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcaatc aatgtctgtt
    33241 ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt
    33301 ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa
    33361 tcattacttt ttttgtcatc tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag
    33421 ccactatggc ccaatatcct accgttgagt tcaatttatt ccgcagaaca tttgtgacag
    33481 atgagaaaac attgatgcag gcactgcaag atgcactaaa agaaaaagcc cttgtcctac
    33541 acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat
    33601 tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca
    33661 agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca
    33721 aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag
    33781 cagatgtcgt tttgctaggc gtatccagaa cttccaaaac gcctttgagc ttgtttttag
    33841 ctaacaaaaa tttaaaggtt gccaatctac ctttgatccc acaagcacat attcccaaac
    33901 agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa
    33961 acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg
    34021 atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct
    34081 gcgtagtaat caatgtcgct tctttatcta tcgaagaaac agcttcactt attttgaatg
    34141 ctttagacct agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac
    34201 agtttttctt gtctttttct taaaaaaact ggactgtgtc agcgaagaag attcgttgat
    34261 acagtccagt ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagcgtc
    34321 tggttcaaaa tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca
    34381 cgatgggact tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat
    34441 tgttccattt ctactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac
    34501 agcagaattt tgagacgagt ggggtcactc aacattttga atagttggct gactttctct
    34561 acttttgttt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    34621 ctaaagttta ccactttttc catcagtctc aagttttttt attcttctgt catttttttg
    34681 ataatcgctt cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca
    34741 ccgatataaa atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt
    34801 tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct
    34861 tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca
    34921 aattctgtcg gtgcttcttc acggaatttt tccatcaaag cagagatttt tgcagagcct
    34981 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa
    35041 atcgtttgca agccgtcata caatgttttg ccttgttttt tatagtaagc tgctacttct
    35101 gctaataaga gcaatgcttg aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa
    35161 ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga
    35221 attttttcag caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt
    35281 tccgcaattg ctgtaggcaa ttcgctagaa acgattgatt tcaataccgc tgcattttca
    35341 ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt
    35401 tggtttcctg tcaatacttc ataggagcca tttggcaaac gaacggctgc gcctaaacgg
    35461 tctgcatcag gatccgttgc aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc
    35521 gcatactcga aagcagagtg ttcttctgga ttcggtgatt tgactgtagt gaaatccggg
    35581 tctgcgacag cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca
    35641 cgttcgccca acatttttcc tgttccgtga agtggtgtgt acacaagctt caaatcttta
    35701 cccattgttt ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg
    35761 gcatccactt cttctccgat gatcgtgatc aatccgctat gttcagcttc gtcgtcagat
    35821 aatacttcaa cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
    35881 tctgcaggag gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct
    35941 ggattgtggg atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa
    36001 gaaagctctg gtgttggacg taaactttcg aatacataag aagggatatt gtgatgtgct
    36061 aatgttttgg ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata
    36121 gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct
    36181 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggtcctaa gattccccgc
    36241 atgcctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
    36301 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
    36361 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
    36421 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
    36481 agaaaagatt cttttatcaa gcacggttac aagtattcgc ataaatcgga ttcagtagat
    36541 ttcacgcaaa aaaaagagta aaagaacaat tgaaaccgtt ctttcactct ttttaaactt
    36601 atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca gcaatcccac
    36661 catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    36721 aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg
    36781 tcaacccagc ttctctaaat ggtgcagtta atggatccaa tcctacatag atgaatactc
    36841 cgtctgctgc caaatcaaac tcattgccag ttttgacatt acgtgctttc acacctgtca
    36901 ctaccatttc atttccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    36961 cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    37021 cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc
    37081 ctcctccgat cactactaga cgtttgttac ggaaaaatgc accatcacaa actgcacagt
    37141 aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    37201 agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt
    37261 tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc
    37321 ttgaaacacc ttcatacatc ttataagcta gatcaggacc catgattgaa tcgaaaccag
    37381 gatagttttc gatttctgcc gtattgttca tttggccgcc aggagcgcct tgttcgatca
    37441 tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggccctg
    37501 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    37561 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    37621 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caatcgttcg
    37681 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg
    37741 aacgggctaa tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt
    37801 tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta
    37861 atagatcgac tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat
    37921 agtcgattcg ataatcatct tccacataac cattctcatc gggcgtatcg actgcaccta
    37981 agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta
    38041 ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga tatggatttt
    38101 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa
    38161 ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg
    38221 catccccagg ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag
    38281 cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgactttc
    38341 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    38401 tacagcctac tttgttctcc ggatctactt catgagcgat cttcgttgcg atggcacttg
    38461 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    38521 aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca
    38581 accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa
    38641 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat
    38701 caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat
    38761 cctcataaaa tttcaaccca gattcatttg gctctttttc atcgcccatt ggaaaaatac
    38821 gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat
    38881 cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat
    38941 caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca
    39001 caacatctac gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg
    39061 ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac
    39121 atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt
    39181 taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc
    39241 atccatgatc cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg
    39301 caaagctgtt ccataatcac cagtcacggc tgcttcgatc gtcagcaatt ccatattctt
    39361 cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc
    39421 gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac
    39481 agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc
    39541 aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg
    39601 tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac
    39661 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    39721 ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    39781 tttgaaaaac gggatgtcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg
    39841 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    39901 gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg
    39961 ttctgtcatc atcgccatta ctggcacatt gcataatccg atcactttat cccatttgcc
    40021 atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattaa tcagccaagc
    40081 atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa
    40141 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    40201 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    40261 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg
    40321 agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc
    40381 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    40441 ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    40501 tgccatgttc tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa
    40561 acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt cttctggatt
    40621 tttgtaaata tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca
    40681 aatcattttc aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt
    40741 attacgtgaa aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt
    40801 tctcttaagc gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg
    40861 ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt
    40921 cagttttcgt aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt
    40981 agatgcgaat cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc
    41041 ttctttagaa cagatgacga ttcaagatac actttctctc attcatcatg accaactgca
    41101 aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa
    41161 tactttgatt tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat
    41221 tgtcaccact tttggtgaat cgttctatga agcgtacaat tgcacaagtg atatgtgtgg
    41281 gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa
    41341 acaaaatcat gtgccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat
    41401 gagtacagcc gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt
    41461 tacgatcaat acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga
    41521 aaattatcaa cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc
    41581 tatcagctca gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa
    41641 agaatagtta agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt
    41701 gcatcgtctg gcccgaccgt ttccccttga ttttccaaaa tatccaaata atccatgtaa
    41761 ttgatgacaa tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg
    41821 aagcgaagca acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa
    41881 tatttccctt ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct
    41941 tcagaaacca tcccgattgc atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa
    42001 ggggcacgta cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt
    42061 gcaaaagctg catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt
    42121 acaatttctt tacctgatct tttctgtcta tcatctgttt catctgcttc ctccttccaa
    42181 aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat caatacatta
    42241 atcacatctg ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctagt
    42301 ccaccttgat tccaagaagt taaattcatt aacccactat acgcgccccc taacgcacca
    42361 ccgacacagg taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc
    42421 tctgtaatcc ccacaatacc agaaacaatg gctggtattg caattgcttt gtcttttgga
    42481 tttctaagct taaagtagag tgcggtaagt gctgcagttt gtccaaaaga cactactgca
    42541 acaggtgtca taatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg
    42601 attgcccaat ggagaccaaa catcaccaga atttgccaga agaagcctac gattgctcca
    42661 taaataactg gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg
    42721 aatgtgaata ctggtccaac gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag
    42781 aatggaacga agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt
    42841 tggctagcta acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca
    42901 atccagggaa tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat
    42961 atggtcgtta gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc
    43021 attaatgctg cgccaagcaa catccctacg aattcattta ctttaaattt acgggcagct
    43081 gttaaaccga taataacagg cataaattgg aatacagaat caccgatatt atcaaacata
    43141 acaaatgtgc cagaaccgcg gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca
    43201 cgaatcatcc cgtcagcagc taggatagca aggaatggtt ggaaacagcc cgatattcca
    43261 tcaataagca tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg
    43321 tttcctgctt ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttga
    43381 tactgccctc ccgctcgcat taccgttaca ataccgtcca tatttttcaa gatctcatca
    43441 tttgcttttt cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca
    43501 ttgatatttt ccttcccgcc gacattttct acgatttttc tcgctaattc ttggtatttt
    43561 cccatttcta ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc
    43621 ttatttcata cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca
    43681 tgcactaaac gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag
    43741 ttgaacgtct cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat
    43801 tttttttgaa taacctgaaa caattcctca gaaacttctc ccacttctga tttttctcca
    43861 tctaggacgc gtgtcagaaa aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa
    43921 tcttcatcaa atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt
    43981 atagtgaaca ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc
    44041 aatgcgataa atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc
    44101 ttcactgttt tttttcccga tggcatactc tttaggaaat agttgtttga tatcatacag
    44161 caatacattt ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc
    44221 cgtcaatgca atatacacag aatcattcaa ctggacttgt agagcctctt ttgtatattc
    44281 aatgatttca caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa
    44341 ctgttgtgaa agcccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat
    44401 cgggtcaccc actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt
    44461 tctttcatcc ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgttttg
    44521 ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc
    44581 catggaacca tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg
    44641 tatcgttttc tttatattat cgaaagcaaa cgcttttgtc aacgctttct tagtggttat
    44701 tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc
    44761 tcacagatga agaatctgcg aagctctgtt ttattttcac tctagcgctt agggcaaaat
    44821 gctactatgc tactgtcaca acctcttttt acacaccact gctttgcagt ttgtacatat
    44881 ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgctct actatatgac
    44941 ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga tgggcaatcg
    45001 ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc ttggatcaat ccttcggttt
    45061 cagtatcgat atttgctgtt gcttcatcca atactagtat tttaggatct gtcacgattg
    45121 ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata gctggcaccg tgttcgatca
    45181 cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga acaaactcag
    45241 ctgcatggcg aatctgttcg tctgtgatag ctggattcat catccggata tttcctgcaa
    45301 tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta cgtaactctt
    45361 ccattggata ttctcgaatg tcatgatcat caatcaagat ttggccttca taaaattcgt
    45421 aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc cccactaatg
    45481 caacggtctc acccggatta gcgacaaaag agatattgtt caatacattg tgctctccat
    45541 cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct tgatctgctc
    45601 caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata cgactaccgg
    45661 caaccatccc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa
    45721 attcctggac ataggtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg
    45781 catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatagaga aaattgatga
    45841 ttggacttaa cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt
    45901 tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc tggataatac
    45961 gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt tcccgcatat
    46021 ttcggtaaat acgagaactg aatttctggt aataccagat gacgattcct aaaatcggca
    46081 agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca acgaaagaag
    46141 aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg
    46201 tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac
    46261 gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga taggtcttca
    46321 atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg actatgactc
    46381 caaacaagta cacaccagca aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca
    46441 gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc acagacaggg
    46501 caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg aacttcatca
    46561 gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat tccgattgat
    46621 aattttcatc catcaggcct cgcctccttc tatcttcgct tctaattgct gtttttccca
    46681 cattcgttca taccagccac ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat
    46741 tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta aacggtgagc
    46801 tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga tagcttcttc
    46861 tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag gatttttgat
    46921 caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca gataaagaga ctccacgttc
    46981 tccaactaag gtatcataac cttttggcaa ttccttgatt tcattgttga taaatgctaa
    47041 agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac ggatattatc
    47101 tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca taaccgattg atcctaataa
    47161 agcattcaat gaatattctt tgatcgaacg tccgccaaat tggattttcc cttgataatc
    47221 atcgtattca cgcatcagta atttgatgat tgtcgttttt cccgcaccag ttttcccaac
    47281 aatacctaat gtttctcctt cttggatcgc aaatttgatt tgttctaatg ctggtgattc
    47341 ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg ccggggtctg
    47401 cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc
    47461 ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca
    47521 aacaagcata ccgatatagc taataaatga gaccatctgc ccgattgaga tcgtgccttc
    47581 caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga taatcaaggt
    47641 aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa taattgcatc
    47701 ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg ttttaatgac
    47761 tttgatccca gtgatacttt cttgtgtctt atcattgata ctagaaaaag cttcttgcga
    47821 atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg
    47881 cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat
    47941 cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag attccagctc ctgctacatt
    48001 ctggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc gatatttttg
    48061 gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc
    48121 caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa gatattgtaa
    48181 gaaagctgca aggatcaata ataagatcca ccaggaaatg atctttattg ataaattctt
    48241 atcggcgatc tcatcaatga tgatcccaat cacttttgga ggaactaact gtaccaaggc
    48301 cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgac gaaaaaacca
    48361 acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag agcataggat
    48421 tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta cgaaataaaa
    48481 aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt ttttcccttg
    48541 gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt gccccattga
    48601 catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta aatagtcttt
    48661 catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag caataatagc
    48721 aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt tccgaattgc
    48781 tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc tttagtttca
    48841 tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac cagcaagaca
    48901 caccacgaaa aaaaattagg cataccgaat ctttttcttt aattttttct ttctgaggtg
    48961 tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc aatccaacaa
    49021 acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag ctgaagtaaa
    49081 tggaacagtg gatgtaccaa agggaaaaag tttttttaaa acacttcttg cttactctgg
    49141 accaggtgca ttagtagcag ttggttatat ggatccaggt aactggtcca cttcgatcac
    49201 tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta gtctaattgc
    49261 tatgctttta cagtatatgg cagcaaaatt aggaatcgtg acccaaatgg atttggccca
    49321 agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct tgactgaatt
    49381 agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc tctacttact
    49441 ttttgatatt ccattggtga ttgccgtctt tattactgta ttagatgttt tcgttctctt
    49501 actactgaca aaagtcgggt ttcgtaagat cgaagcactg gttgtctgct taattttcgt
    49561 cattttattc gtttttgtct atcaagttgg cttgtctaac ccagattggc acagtttatt
    49621 tgctggcttt atccctaaca gtcatacatt ctcagactca ccggtcatcg gcggcgaaac
    49681 tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata acttatatct
    49741 tcattcagca gtttcacaaa ctcggaagat cgatcaccaa aacgaagaag atgtagcaca
    49801 agctgtacgt ttttctactt gggattctaa tatccagttg tcaatggctt ttctagtcaa
    49861 tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc
    49921 cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg gtattttgat
    49981 tgctgtagca aaatctggta tcttgtccac tttatttgct gtagcacttc ttgcttctgg
    50041 ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag gattcatcca
    50101 tatgcgtatc ccgatttggc taagaagact ggtcacacga ttgctttccg tgattcctgt
    50161 attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacatg ccgctttgaa
    50221 taacttaatg aatgaatccc aggtgttctt ggcttttgcg ttgccatttt cgatgattcc
    50281 tttactgatg atgaccaaca gccaagcgga gatgggtagc cgatttaaaa attcattcat
    50341 cgtaaaactt cttggctggt tctctgtgat tagtctaact tacttgaact tacgaggatt
    50401 accaggacag atcgaaagtt tcttcggcga gcaagccact tccgctcaac tagcattggc
    50461 tgatcacatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat ggacgattgg
    50521 tgatttatat aaaggaaaca aaaaatatga acagcttttg caggaaacgg tagaagaaat
    50581 cgaataaaaa ctgatttttc cacacaaaga cccttgatag ttaggttaaa aatctagcta
    50641 ccaagggtct ttttatttag aagataaagc ttttttcaga tttaatttac tgaaaactag
    50701 catgcttgtt actacgatga attgcgttaa tggcacgcct gcaccagtgt agacacttaa
    50761 aaaatagtcc tttttttctg gcgcataaga acaaaaccat gagacaccga aaagaaaaac
    50821 caattgtgtc agtattaatc cggtgaccac aatcaaagag gaactataat aggcacgatc
    50881 agcttttgca aatacggttc ctagtatcat aagtaatatc cctgctattg aagcaatcag
    50941 cacgggaata gccagaactt ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt
    51001 tgttaattgg aaattaggtg ttctcggttc attactatcg atccaattct ccattagcgg
    51061 aaggtttaat aattcttgag cctgtttgac ttgttcttgc gttccgaaac tataatttcc
    51121 tccttgatgg aaatctttat atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa
    51181 tacgattggc agatcactca tcgtctttcc atctcctgat cccatcagat agaactcttt
    51241 tttctttttg atggttccct tgtgataggc ttcttctaat tcttggttga cttttttcca
    51301 aaaatcattg gtttcttttg cattgttttg gtaataacca gattcgctta ccacatctcg
    51361 caatgcccaa aagatgatat ctccgggtaa ttcttttact tttccacctt gagtccacgc
    51421 gtgagtcgta tataattcgt caatacgggt agaaacagta tggaatgtag gagatgcggc
    51481 taatgcttta tttagtgcag catgtgagac ccatatctca ctgttttctt tattcaagtt
    51541 cgtttcatca tctattcgga tcagttgttt catcaaacgt ccaaattctc cactggaacg
    51601 gtcattgatg gttgcaatac cgtatgttgt ctcattttta tattcgatcc actgatagac
    51661 gatggaaaag ccaactatag gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga
    51721 gagaagaaag agtcgttttt ttaacttctt tacttctttg aaagaaaagt cttgccctag
    51781 caagatctgg acgaaactga tacccaaagc tactacagta aaaggaagaa gccagataga
    51841 atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa agccctaaaa accatggcag
    51901 caattttctg ctagaaccgt atttgcggta ataaatagct aataaacaag cgataatgat
    51961 ctcaacagcc ggaatcacga gggcattacg atatgttcga gtggaatatt catgtgtaaa
    52021 actaatcgga gaaaacaaaa aataaaaata gatacttaaa agaagatact tgttcttcac
    52081 aatcggttta aacgcgagtg caaccaatat actggcaaaa atactgaaca tgccgaacaa
    52141 aactgcataa gaaagatgca gtttatgtgc aagtactaaa aataagctgt aggagattcc
    52201 tttggaaagt gttgtagtcg tatactcccc tagccattta ccatccaaaa tattttgagc
    52261 atagtgaatc agcagctgat catcataggt cgccgacata tctgttccat agattgcttt
    52321 actatataaa tacaaccgaa taattgtaaa taatacggct atgccaagaa taaagtatgt
    52381 atgtttcttt tttagttggt cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt
    52441 tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt tttacttttc tatcagtata
    52501 ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa
    52561 aagcactcct ttgtttctta aacaaaaaag gacaaggcag actgcccaat ccttttttgt
    52621 tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct aataactcag ggaacacttc
    52681 gggatcgaat gcttgcgctt tgaacatgga aatttctttt ccatatggtg cgtaagcttt
    52741 ctttttatct ttaccgacat atggggtttc taatattttc gaaatgttgt caaacattgg
    52801 atcatggacg attttgttca atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg
    52861 ccgatcttta tgagaacctt gtggattttt agaatcattg atatgcagta ctttaagccg
    52921 atctaatccg atgaccttat caaactcttc cattacacct gaaaaatctt cttttatatt
    52981 atatccggca tcattcgtat gacatgtatc aaaagtaacg gataattttt cattcaattt
    53041 tactccatcg atgatttgcg ctaattcctc aaatgttcga ccgatttccg tacctttccc
    53101 tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc
    53161 ctcaataatt ctagcaatcc cagcttctgc ccctgctcct acatgagcgc ctgggtgaag
    53221 ggtgatttga gtagcaccta aagcttccgc acggacaatc tcttctctca gaaattgtac
    53281 agcaaatgga aacatttctg gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg
    53341 aacgacgata tttgttagtc cgttttctgc cataaatgct tttccggcat caatattcat
    53401 ctcttcgata gcttttcttc tagtattttg aggagcgcct gtatagatca taaaggtcga
    53461 agatccgtaa ctggctgctt cttctgccgc gcctaacagc attttctttc cgctcatgct
    53521 gacatgtgag cctaataaca tggatggatt cctccttgaa aggcaattgt ttcgaacaat
    53581 tcctgtatat tgataagatg atgtacaaag aaaatggttt gtttttcttt gccctgttct
    53641 ccatagtaac tgaaactttc cctaaagaaa agcttaagaa ctctttcata aaaaagatca
    53701 tttcctcacg ttttatctca ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt
    53761 tttttgtcct attatttgct ataattagcg aaataaaacc attagttgag gtgacccttt
    53821 tgccacgcaa aaatactcga aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc
    53881 ctaagattat ttttagagtg tttcaatcat tgactgtttt tattactgtt cttcttttgt
    53941 tgttcgcagc tctaggtatc ggaataggtg ctggctactt tgcctatctt gtcgaagaca
    54001 cacaattgcc gactaaaaaa gaactgcaga cagaactagg aaacattacg gaaacgtcaa
    54061 agattgttta tgcagataat acagagatat caaaaatcca gacagatctg atgcggacga
    54121 ctatttcatc tgataagatt tcgcctttat tgaaaaccgc gattatcagt acagaagatg
    54181 aatacttcga taaacatcaa ggttatgtcc ctaaagctgt tttacgagca ctagtttcag
    54241 aagcgactgg catcggctcg tctggaggat ccactttgac acagcaatta gtcaaacaac
    54301 agatcctaac cgatgaaaca acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac
    54361 aagtagaaaa acacttttca aaaaatgaga tcattactac gtatttgaat gtctcaccat
    54421 ttggtcggaa taataagggg caaaatattg ctggagtcca ggaagctgct agaggaatct
    54481 ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc ttatatcgct ggtttgcctc
    54541 aaagccctat cacgtatagt ccatacacga atacaggcgc gctaaaagaa gatctttctg
    54601 ccggtcttgc cagaaaagat tttgttttat tcagtatgta ccgtgaagga caaattacga
    54661 aagaacaata cgaagaagcg aaagcctatg atcttacaaa agactttctc ccacaacaaa
    54721 tcgcggaaca gactgacaga gaattcttgt attataccgt gatgaacgaa gcaacaagga
    54781 ttattgcaca acaattggca gaaaaagata atgctgatat gtcagattca tcggtcagtg
    54841 aagcttatta tcaaaaagca cagcaaacga tccaaaacaa aggatatacg atccattcga
    54901 cgatcgataa agatatctat gctgcattac aaaccggcgt agaaaattat ggctacatgt
    54961 tagatgatgg tgctggtagc caagtggaga ctggaaatgt attgatggat aacagaaccg
    55021 gacgtatcta tggcttcgtg ggtggtcgga attacagcca gaatcaaaat aaccacgcct
    55081 ttgatacaga acgtcaagct ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa
    55141 tcgatatggg acttgtcgga tcagaatccc gtgtatcaga ttatgcaacg acttggcaag
    55201 aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc
    55261 aaaccgtccg tgagtcactg gaatggtcca acaatatccc agcttatcac ttatatcaag
    55321 atgtgttgaa ttccggtgga tcgaaacaat acgcttacga gcactacttg gcgaagatga
    55381 actacccagc aaatgataat tggggcgttg aatcggctcc tttagggaca gtagatgtaa
    55441 cgacgcttca acagacaaat ggattccagg ccttagcaaa tggtggagtc tacgaagaag
    55501 gctatatcat tgattccatc acagataatg caggtaatgt gatttataaa catgaaagta
    55561 attctgtccg tatttattcc gaagcaacgg catcgatcat gaatgacatg atgcgtagtg
    55621 tgatcaatga aaaaatcact acaccattca aagatgtcat ctcaagctta aacggaaatc
    55681 taggaaatgc agattgggtc ggaaaaactg gttctaccaa tgaataccga gattcatggc
    55741 tgatcgtctc aactccatct atcactatca gtagttgggc aggacacgat gacaatactg
    55801 gaatggacag ccaagcaaga atacgttcgg ctaattatct tgccaatcta atcaatcaag
    55861 cttaccaagc aaaccctgag attttcggca ctgatgagaa atttgactta tcatccgatg
    55921 tgatgaagaa aaaagtatct gggttcacag gacaaacagc tggaaaagtt actgtcaata
    55981 aaaaatccat ccaaacgcca ggaaaaacag tggagtcact ttgggcaaaa aatggtccga
    56041 agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga aaactataca gattactgga
    56101 acaccgcttc agcttatgct agagcgaatc cagagaccaa agaatcagac gataaagatg
    56161 attgatctaa tagattaatc agaattaaag ggagctgcga caaaaatctt gtcacagctc
    56221 cctttttccg aataaatggt gttcaaaagc ttacatctac gatattaaga caaatttatg
    56281 taaaaa
//
LOCUS       DACELF010000006        52646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.006, whole genome shotgun sequence.
ACCESSION   DACELF010000006 DACELF010000000
VERSION     DACELF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.006"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..971
                     /locus_tag="IW687_000373"
     CDS             117..971
                     /locus_tag="IW687_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0949665.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IW687_000374"
     CDS             972..2309
                     /locus_tag="IW687_000374"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0949666.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IW687_000375"
     CDS             2314..2538
                     /locus_tag="IW687_000375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0949667.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IW687_000376"
     CDS             2540..3430
                     /locus_tag="IW687_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0949668.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IW687_000377"
     CDS             3439..4257
                     /locus_tag="IW687_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0949669.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IW687_000378"
     CDS             4364..4813
                     /locus_tag="IW687_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0949670.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IW687_000379"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IW687_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0949671.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IW687_000380"
     CDS             complement(6568..6684)
                     /locus_tag="IW687_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0949672.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IW687_000381"
     CDS             complement(6757..7713)
                     /locus_tag="IW687_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0949673.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IW687_000382"
     CDS             7884..8252
                     /locus_tag="IW687_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0949674.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IW687_000383"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IW687_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0949675.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IW687_000384"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IW687_000384"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0949676.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IW687_000385"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IW687_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0949677.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IW687_000386"
     CDS             10240..12432
                     /locus_tag="IW687_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0949678.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IW687_000387"
     CDS             12458..13420
                     /locus_tag="IW687_000387"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0949679.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IW687_000388"
     CDS             13434..14795
                     /locus_tag="IW687_000388"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0949680.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IW687_000389"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IW687_000389"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0949681.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IW687_000390"
     CDS             15924..17144
                     /locus_tag="IW687_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0949682.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IW687_000391"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IW687_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0949683.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW687_000392"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW687_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0949684.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IW687_000393"
     CDS             19906..20583
                     /locus_tag="IW687_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0949685.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IW687_000394"
     CDS             20597..21196
                     /locus_tag="IW687_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0949686.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IW687_000395"
     CDS             21211..21498
                     /locus_tag="IW687_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0949687.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IW687_000396"
     CDS             21547..22329
                     /locus_tag="IW687_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0949688.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IW687_000397"
     CDS             22350..23057
                     /locus_tag="IW687_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0949689.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IW687_000398"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IW687_000398"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0949690.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IW687_000399"
     CDS             complement(26166..27692)
                     /locus_tag="IW687_000399"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0949691.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IW687_000400"
     CDS             complement(27707..28354)
                     /locus_tag="IW687_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0949692.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IW687_000401"
     CDS             complement(28538..28867)
                     /locus_tag="IW687_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0949693.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IW687_000402"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IW687_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0949694.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IW687_000403"
     CDS             29788..30801
                     /locus_tag="IW687_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0949695.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IW687_000404"
     CDS             30801..32078
                     /locus_tag="IW687_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0949696.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IW687_000405"
     CDS             32141..34843
                     /locus_tag="IW687_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0949697.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IW687_000406"
     CDS             34995..35312
                     /locus_tag="IW687_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0949698.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IW687_000407"
     CDS             35342..35662
                     /locus_tag="IW687_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0949699.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IW687_000408"
     CDS             35748..37208
                     /locus_tag="IW687_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0949700.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IW687_000409"
     CDS             37276..37497
                     /locus_tag="IW687_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949701.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IW687_000410"
     CDS             37528..37710
                     /locus_tag="IW687_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0949702.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IW687_000411"
     CDS             37710..38123
                     /locus_tag="IW687_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0949703.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IW687_000412"
     CDS             38246..39427
                     /locus_tag="IW687_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0949704.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IW687_000413"
     tRNA            39816..39889
                     /locus_tag="IW687_000413"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39958..41097)
                     /locus_tag="IW687_000414"
     CDS             complement(39958..41097)
                     /locus_tag="IW687_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0949705.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41396..42031)
                     /locus_tag="IW687_000415"
     CDS             complement(41396..42031)
                     /locus_tag="IW687_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949706.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42144..42779)
                     /locus_tag="IW687_000416"
     CDS             complement(42144..42779)
                     /locus_tag="IW687_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ0949707.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42813..43274)
                     /locus_tag="IW687_000417"
     CDS             complement(42813..43274)
                     /locus_tag="IW687_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ0949708.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43404..43835)
                     /locus_tag="IW687_000418"
     CDS             complement(43404..43835)
                     /locus_tag="IW687_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0949709.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43853..44173)
                     /locus_tag="IW687_000419"
     CDS             complement(43853..44173)
                     /locus_tag="IW687_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0949710.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44472..45248
                     /locus_tag="IW687_000420"
     CDS             44472..45248
                     /locus_tag="IW687_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0949711.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45263..45466
                     /locus_tag="IW687_000421"
     CDS             45263..45466
                     /locus_tag="IW687_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0949712.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45482..45820
                     /locus_tag="IW687_000422"
     CDS             45482..45820
                     /locus_tag="IW687_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0949713.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45807..45986
                     /locus_tag="IW687_000423"
     CDS             45807..45986
                     /locus_tag="IW687_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949714.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46029..46499)
                     /locus_tag="IW687_000424"
     CDS             complement(46029..46499)
                     /locus_tag="IW687_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949715.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46586..47284
                     /locus_tag="IW687_000425"
     CDS             46586..47284
                     /locus_tag="IW687_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0949716.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47462..47803
                     /locus_tag="IW687_000426"
     CDS             47462..47803
                     /locus_tag="IW687_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949717.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47796..48467
                     /locus_tag="IW687_000427"
     CDS             47796..48467
                     /locus_tag="IW687_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0949718.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48473..49159
                     /locus_tag="IW687_000428"
     CDS             48473..49159
                     /locus_tag="IW687_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949719.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49162..49911
                     /locus_tag="IW687_000429"
     CDS             49162..49911
                     /locus_tag="IW687_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0949720.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49923..50192
                     /locus_tag="IW687_000430"
     CDS             49923..50192
                     /locus_tag="IW687_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949721.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50354..50656
                     /locus_tag="IW687_000431"
     CDS             50354..50656
                     /locus_tag="IW687_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949722.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            50653..50814
                     /locus_tag="IW687_000432"
     CDS             50653..50814
                     /locus_tag="IW687_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0949723.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            50811..51116
                     /locus_tag="IW687_000433"
     CDS             50811..51116
                     /locus_tag="IW687_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949724.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            51116..51472
                     /locus_tag="IW687_000434"
     CDS             51116..51472
                     /locus_tag="IW687_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0949725.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            51432..51677
                     /locus_tag="IW687_000435"
     CDS             51432..51677
                     /locus_tag="IW687_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949726.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            51674..52093
                     /locus_tag="IW687_000436"
     CDS             51674..52093
                     /locus_tag="IW687_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0949727.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            52090..>52646
                     /locus_tag="IW687_000437"
     CDS             52090..>52646
                     /locus_tag="IW687_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0949728.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
BASE COUNT        17792 a         8801 c        10972 g        15081 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    40081 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    40141 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    40201 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    40261 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    40321 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    40381 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    40441 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    40501 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    40561 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    40621 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    40681 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    40741 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    40801 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    40861 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    40921 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    40981 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    41041 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    41101 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    41161 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    41221 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    41281 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    41341 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    41401 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    41461 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    41521 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    41581 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    41641 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    41701 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    41761 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    41821 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    41881 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    41941 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    42001 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    42061 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    42121 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    42181 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    42241 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    42301 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    42361 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    42421 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    42481 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    42541 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    42601 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    42661 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    42721 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    42781 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    42841 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    42901 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    42961 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    43021 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    43081 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    43141 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    43201 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    43261 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    43321 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    43381 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    43441 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    43501 tccataaatt tttcgagatt aaactgagat ttatcagtca gttcgttctc aatatagata
    43561 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    43621 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    43681 tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt
    43741 ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg
    43801 tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat
    43861 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    43921 ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc
    43981 tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta
    44041 attcatctga attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa
    44101 aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt
    44161 cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat
    44221 acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg taaacagatt
    44281 taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag
    44341 ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca
    44401 gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag
    44461 gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa
    44521 ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt
    44581 caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa
    44641 tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt
    44701 taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag
    44761 tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt
    44821 tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa
    44881 gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca
    44941 tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc
    45001 cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat
    45061 ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc
    45121 cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc
    45181 tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa
    45241 ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga
    45301 aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag
    45361 atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga
    45421 agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct
    45481 aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt
    45541 ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt
    45601 gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag
    45661 atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga
    45721 aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt
    45781 ttcggaaatc ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg
    45841 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
    45901 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
    45961 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
    46021 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
    46081 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
    46141 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
    46201 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
    46261 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
    46321 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
    46381 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
    46441 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
    46501 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
    46561 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
    46621 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
    46681 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
    46741 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
    46801 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
    46861 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
    46921 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
    46981 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
    47041 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
    47101 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
    47161 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
    47221 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
    47281 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
    47341 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
    47401 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
    47461 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
    47521 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
    47581 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
    47641 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
    47701 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
    47761 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
    47821 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
    47881 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
    47941 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
    48001 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
    48061 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
    48121 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
    48181 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
    48241 gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
    48301 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
    48361 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
    48421 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
    48481 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc
    48541 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
    48601 tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa
    48661 cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg
    48721 gaaggaattc ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga
    48781 cgaatcgaat acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat
    48841 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata
    48901 ttacttctac aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga
    48961 catcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga
    49021 gtacacattt gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat
    49081 taatttcaaa aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa
    49141 gttaaatatt ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc
    49201 aacgtaaggt acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg
    49261 gccttgtgta atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat
    49321 ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg
    49381 aatcgggaaa tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt
    49441 aatcaatacc ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat
    49501 aatacatcta ttaataatac atttaataat acaaaagaat atataagaga gttaccgcct
    49561 tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt
    49621 ttgtcagatg agcagatgga gaaactcaaa acagaattcc ctaatgatta ccaagagcga
    49681 atcgaacgac tgtcagagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca
    49741 actattcgaa gttgggcaag gaaagaaaaa agtgaaccta agaacgcaag cagtggatac
    49801 aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga agcctatctc
    49861 aagaaaaaag cgctagaacg agctaataga caatcaaaag caccattcta agaggtggaa
    49921 aaatgaagat cgattatcta gaactaatta atgaaatagc gaattataaa aagggcgagg
    49981 aattagacgt cctgagagac gtatatgatc aactcgaaga agctggaatt gaaggaatta
    50041 agaatgatcg ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa
    50101 cacaattaag aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc
    50161 aaaaacatct taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca
    50221 cagattgaac taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg
    50281 ctcaacacgt tccatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa
    50341 ggagaatgtc gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc
    50401 agaagattgg caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat
    50461 gtgggcagac aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa
    50521 acttctagtg atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat
    50581 cgtgaggaaa agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt
    50641 ggaggaatca tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt
    50701 gagattgacg atctggaacg aacaaaggca gtgatggtca atgaaacggc gagaaaggca
    50761 atcgatttgc acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg
    50821 ataagaaagc agcaatgaaa agaattgctg aattaaccaa gtcagaatct tggcaagaag
    50881 acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca
    50941 aacggagaac gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta
    51001 ctgctgaaca gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta
    51061 ggagcttatg gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga
    51121 tctcattaca caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga
    51181 taaaaaatca aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg
    51241 agtgcgaagt agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat
    51301 tcaaaaaggg ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact
    51361 tcatcaagat cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag
    51421 gaggacagca aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat
    51481 caggagcaac actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc
    51541 agattatttt gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag
    51601 aatcaaaagc ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg
    51661 aggaggacag cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag
    51721 agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat
    51781 aaatcctacc gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg
    51841 aatgaaagat aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg
    51901 aaatggcttc gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc
    51961 cggattagtt tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct
    52021 gtttgaagaa tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga
    52081 ggaacagcga tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga
    52141 aatgatgcaa tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa
    52201 aaaatagacg aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa
    52261 tctacggacc cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt
    52321 gattcatatt atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga
    52381 gaagaattac tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac
    52441 ggctacgagg ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt
    52501 actaaatgtg ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct
    52561 catcgattgc ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga
    52621 acagtggaag aggtggtaga aagatg
//
LOCUS       DACELF010000007        51323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.007, whole genome shotgun sequence.
ACCESSION   DACELF010000007 DACELF010000000
VERSION     DACELF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.007"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            320..1165
                     /locus_tag="IW687_000438"
     CDS             320..1165
                     /locus_tag="IW687_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0949729.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1446..2666
                     /locus_tag="IW687_000439"
     CDS             1446..2666
                     /locus_tag="IW687_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0949730.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2663..5383
                     /locus_tag="IW687_000440"
     CDS             2663..5383
                     /locus_tag="IW687_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0949731.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5367..6311
                     /locus_tag="IW687_000441"
     CDS             5367..6311
                     /locus_tag="IW687_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0949732.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6380..7390)
                     /locus_tag="IW687_000442"
     CDS             complement(6380..7390)
                     /locus_tag="IW687_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0949733.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7570..7914)
                     /locus_tag="IW687_000443"
     CDS             complement(7570..7914)
                     /locus_tag="IW687_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0949734.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7907..8341)
                     /locus_tag="IW687_000444"
     CDS             complement(7907..8341)
                     /locus_tag="IW687_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0949735.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8479..9216
                     /locus_tag="IW687_000445"
     CDS             8479..9216
                     /locus_tag="IW687_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949736.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9209..10420
                     /locus_tag="IW687_000446"
     CDS             9209..10420
                     /locus_tag="IW687_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949737.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10627..11406
                     /locus_tag="IW687_000447"
     CDS             10627..11406
                     /locus_tag="IW687_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0949738.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11476..12138
                     /gene="trmB"
                     /locus_tag="IW687_000448"
     CDS             11476..12138
                     /gene="trmB"
                     /locus_tag="IW687_000448"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0949739.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12236..13990)
                     /gene="ade"
                     /locus_tag="IW687_000449"
     CDS             complement(12236..13990)
                     /gene="ade"
                     /locus_tag="IW687_000449"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0949740.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14122..15051
                     /gene="cpaF"
                     /locus_tag="IW687_000450"
     CDS             14122..15051
                     /gene="cpaF"
                     /locus_tag="IW687_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0949741.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15008..16057
                     /locus_tag="IW687_000451"
     CDS             15008..16057
                     /locus_tag="IW687_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0949742.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16054..16332
                     /locus_tag="IW687_000452"
     CDS             16054..16332
                     /locus_tag="IW687_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0949743.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16307..16777
                     /locus_tag="IW687_000453"
     CDS             16307..16777
                     /locus_tag="IW687_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0949744.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16794..17096
                     /locus_tag="IW687_000454"
     CDS             16794..17096
                     /locus_tag="IW687_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949745.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17113..17484
                     /locus_tag="IW687_000455"
     CDS             17113..17484
                     /locus_tag="IW687_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0949746.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17456..17815
                     /locus_tag="IW687_000456"
     CDS             17456..17815
                     /locus_tag="IW687_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949747.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17987..18994
                     /locus_tag="IW687_000457"
     CDS             17987..18994
                     /locus_tag="IW687_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0949748.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19019..20203
                     /locus_tag="IW687_000458"
     CDS             19019..20203
                     /locus_tag="IW687_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0949749.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20640..22223)
                     /locus_tag="IW687_000459"
     CDS             complement(20640..22223)
                     /locus_tag="IW687_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0949750.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22801..22944)
                     /locus_tag="IW687_000460"
     CDS             complement(22801..22944)
                     /locus_tag="IW687_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0949751.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23166..23645)
                     /locus_tag="IW687_000461"
     CDS             complement(23166..23645)
                     /locus_tag="IW687_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0949752.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23718..24002)
                     /locus_tag="IW687_000462"
     CDS             complement(23718..24002)
                     /locus_tag="IW687_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949753.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24080..24274)
                     /gene="ssrS"
                     /locus_tag="IW687_000463"
     ncRNA           complement(24080..24274)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW687_000463"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24346..25632)
                     /locus_tag="IW687_000464"
     CDS             complement(24346..25632)
                     /locus_tag="IW687_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0949754.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25770..26441
                     /locus_tag="IW687_000465"
     CDS             25770..26441
                     /locus_tag="IW687_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0949755.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26407..26895
                     /locus_tag="IW687_000466"
     CDS             26407..26895
                     /locus_tag="IW687_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0949756.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26908..27855
                     /gene="prmA"
                     /locus_tag="IW687_000467"
     CDS             26908..27855
                     /gene="prmA"
                     /locus_tag="IW687_000467"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0949757.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27856..28620
                     /locus_tag="IW687_000468"
     CDS             27856..28620
                     /locus_tag="IW687_000468"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0949758.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28853..31066
                     /locus_tag="IW687_000469"
     CDS             28853..31066
                     /locus_tag="IW687_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0949759.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31077..31523
                     /locus_tag="IW687_000470"
     CDS             31077..31523
                     /locus_tag="IW687_000470"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0949760.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31694..32953
                     /locus_tag="IW687_000471"
     CDS             31694..32953
                     /locus_tag="IW687_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0949761.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33042..33989)
                     /locus_tag="IW687_000472"
     CDS             complement(33042..33989)
                     /locus_tag="IW687_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0949762.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34383..35675
                     /locus_tag="IW687_000473"
     CDS             34383..35675
                     /locus_tag="IW687_000473"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0949763.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35690..37459
                     /gene="aspS"
                     /locus_tag="IW687_000474"
     CDS             35690..37459
                     /gene="aspS"
                     /locus_tag="IW687_000474"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0949764.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37568..38443
                     /locus_tag="IW687_000475"
     CDS             37568..38443
                     /locus_tag="IW687_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0949765.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38709..40592
                     /locus_tag="IW687_000476"
     CDS             38709..40592
                     /locus_tag="IW687_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0949766.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40667..40951)
                     /locus_tag="IW687_000477"
     CDS             complement(40667..40951)
                     /locus_tag="IW687_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0949767.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40941..41675)
                     /locus_tag="IW687_000478"
     CDS             complement(40941..41675)
                     /locus_tag="IW687_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0949768.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41804..42427
                     /locus_tag="IW687_000479"
     CDS             41804..42427
                     /locus_tag="IW687_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0949769.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42619..43740
                     /locus_tag="IW687_000480"
     CDS             42619..43740
                     /locus_tag="IW687_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0949770.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43747..46875
                     /locus_tag="IW687_000481"
     CDS             43747..46875
                     /locus_tag="IW687_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0949771.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46894..50088
                     /locus_tag="IW687_000482"
     CDS             46894..50088
                     /locus_tag="IW687_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0949772.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50234..50941)
                     /locus_tag="IW687_000483"
     CDS             complement(50234..50941)
                     /locus_tag="IW687_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0949773.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16799 a         8958 c        10643 g        14923 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct
      721 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
      781 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      841 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      901 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      961 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
     1021 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
     1081 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
     1141 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
     1201 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
     1261 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
     1321 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
     1381 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
     1441 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
     1501 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
     1561 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
     1621 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
     1681 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1741 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1801 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1861 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1921 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1981 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     2041 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     2101 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     2161 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     2221 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     2281 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     2341 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     2401 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     2461 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     2521 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     2581 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     2641 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     2701 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2761 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2821 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2881 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2941 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     3001 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     3061 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     3121 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     3181 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     3241 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     3301 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     3361 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     3421 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     3481 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     3541 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     3601 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     3661 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3721 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3781 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3841 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3901 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3961 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     4021 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     4081 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     4141 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     4201 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     4261 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     4321 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     4381 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     4441 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     4501 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     4561 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     4621 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     4681 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4741 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4801 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4861 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4921 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4981 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     5041 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     5101 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     5161 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     5221 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     5281 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     5341 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     5401 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     5461 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     5521 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     5581 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     5641 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     5701 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5761 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5821 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5881 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5941 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     6001 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     6061 tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt ttagcacacc
     6121 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     6181 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     6241 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     6301 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     6361 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     6421 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     6481 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     6541 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     6601 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     6661 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     6721 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     6781 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     6841 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     6901 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     6961 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     7021 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     7081 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     7141 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     7201 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     7261 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     7321 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     7381 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     7441 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     7501 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     7561 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     7621 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     7681 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     7741 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     7801 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     7861 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     7921 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     7981 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     8041 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     8101 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     8161 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     8221 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     8281 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     8341 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     8401 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     8461 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     8521 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     8581 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     8641 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     8701 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     8761 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     8821 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     8881 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     8941 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     9001 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     9061 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     9121 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     9181 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     9241 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     9301 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     9361 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     9421 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     9481 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     9541 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     9601 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     9661 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     9721 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     9781 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     9841 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     9901 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     9961 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
    10021 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
    10081 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
    10141 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
    10201 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
    10261 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
    10321 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
    10381 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
    10441 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
    10501 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
    10561 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
    10621 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
    10681 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
    10741 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
    10801 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
    10861 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
    10921 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
    10981 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
    11041 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
    11101 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
    11161 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
    11221 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
    11281 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
    11341 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
    11401 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
    11461 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
    11521 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
    11581 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
    11641 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    11701 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    11761 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    11821 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    11881 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    11941 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    12001 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    12061 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    12121 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    12181 aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    12241 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    12301 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    12361 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    12421 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    12481 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    12541 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    12601 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    12661 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    12721 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    12781 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    12841 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    12901 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    12961 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    13021 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    13081 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    13141 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    13201 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    13261 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    13321 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    13381 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    13441 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    13501 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    13561 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    13621 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    13681 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    13741 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    13801 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    13861 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    13921 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    13981 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggctt
    14041 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    14101 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    14161 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    14221 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    14281 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    14341 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    14401 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    14461 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    14521 cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct aatgtatcgg
    14581 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    14641 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    14701 aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    14761 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    14821 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    14881 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    14941 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    15001 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    15061 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    15121 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    15181 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    15241 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    15301 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    15361 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    15421 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    15481 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    15541 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    15601 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    15661 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    15721 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    15781 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    15841 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    15901 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    15961 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    16021 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    16081 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    16141 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    16201 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    16261 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    16321 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    16381 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    16441 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    16501 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    16561 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    16621 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    16681 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    16741 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    16801 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    16861 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    16921 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    16981 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    17041 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    17101 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    17161 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    17221 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    17281 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    17341 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    17401 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    17461 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    17521 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    17581 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    17641 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    17701 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    17761 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    17821 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    17881 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    17941 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    18001 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    18061 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    18121 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    18181 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    18241 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    18301 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    18361 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    18421 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    18481 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    18541 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    18601 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    18661 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    18721 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    18781 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    18841 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    18901 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    18961 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    19021 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    19081 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    19141 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    19201 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    19261 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    19321 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    19381 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    19441 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    19501 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    19561 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    19621 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    19681 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    19741 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    19801 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    19861 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    19921 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    19981 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    20041 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    20101 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    20161 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    20221 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    20281 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    20341 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    20401 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    20461 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    20521 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    20581 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    20641 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    20701 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    20761 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    20821 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    20881 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    20941 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    21001 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    21061 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    21121 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    21181 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    21241 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    21301 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    21361 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    21421 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    21481 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    21541 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    21601 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    21661 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    21721 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acggtgtgga
    21781 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    21841 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    21901 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    21961 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    22021 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    22081 aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc aattttttca
    22141 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    22201 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    22261 tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    22321 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    22381 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    22441 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    22501 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    22561 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    22621 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    22681 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    22741 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    22801 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    22861 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    22921 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    22981 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat tttacttgaa
    23041 ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt
    23101 cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct
    23161 acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca
    23221 acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa
    23281 cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct
    23341 gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta
    23401 ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca
    23461 gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca
    23521 atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta
    23581 aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt
    23641 aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta
    23701 gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg
    23761 aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg
    23821 aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc gtaaaattgt
    23881 aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta
    23941 aataaataat agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc
    24001 atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg
    24061 ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat
    24121 cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc
    24181 atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca
    24241 cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta
    24301 aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg
    24361 atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc
    24421 tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt
    24481 gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt
    24541 cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg
    24601 aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca
    24661 aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc
    24721 agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc
    24781 ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg
    24841 ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga
    24901 aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca
    24961 ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag
    25021 tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag
    25081 gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc
    25141 ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa
    25201 gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc
    25261 tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt
    25321 atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc
    25381 cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata
    25441 attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata
    25501 tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc
    25561 gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa
    25621 tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt
    25681 agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa
    25741 tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt
    25801 tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg
    25861 acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag
    25921 aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg
    25981 aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta
    26041 ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga
    26101 ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg
    26161 gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata
    26221 gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta
    26281 gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg
    26341 gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga
    26401 aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg
    26461 acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt
    26521 cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg
    26581 caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt
    26641 tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa
    26701 tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat
    26761 ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat
    26821 caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg
    26881 tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa
    26941 agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg
    27001 attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat
    27061 aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg
    27121 actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt
    27181 ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca
    27241 acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca
    27301 agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt
    27361 atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca
    27421 gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt
    27481 gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt
    27541 cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct
    27601 aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca
    27661 gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg
    27721 atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa
    27781 gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa
    27841 ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat
    27901 tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag
    27961 gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat cacgaagatt
    28021 actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca
    28081 agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag
    28141 aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt
    28201 aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag
    28261 gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa
    28321 gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca
    28381 aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga
    28441 atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta
    28501 gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct
    28561 ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa
    28621 cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt
    28681 ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa
    28741 agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag
    28801 atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa
    28861 agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga
    28921 acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt
    28981 tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga
    29041 tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac
    29101 agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga
    29161 cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa
    29221 ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc
    29281 tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat
    29341 cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat
    29401 caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat
    29461 cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga
    29521 agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa
    29581 acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta
    29641 tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg
    29701 agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc
    29761 aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt
    29821 agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca
    29881 ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt
    29941 aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat
    30001 ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga
    30061 cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga
    30121 tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa
    30181 attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg
    30241 cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa
    30301 agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac
    30361 agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga
    30421 tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag
    30481 ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca
    30541 gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa
    30601 agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag
    30661 tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg
    30721 gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga
    30781 acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga
    30841 tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa
    30901 tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac
    30961 gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat
    31021 caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga
    31081 gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa
    31141 aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg
    31201 tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa
    31261 tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt
    31321 ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag
    31381 ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta
    31441 ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca
    31501 ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac
    31561 gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc
    31621 ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag
    31681 gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa
    31741 attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt
    31801 cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa
    31861 agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa
    31921 gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa
    31981 aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga
    32041 taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag
    32101 taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc
    32161 taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac
    32221 ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca
    32281 aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata
    32341 tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt
    32401 tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg
    32461 acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt
    32521 acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc
    32581 gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat
    32641 tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat
    32701 gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat
    32761 tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct
    32821 tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga
    32881 acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact
    32941 ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc
    33001 ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct
    33061 tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca
    33121 gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg
    33181 taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt
    33241 acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt
    33301 cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa
    33361 ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat
    33421 aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat
    33481 ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg
    33541 atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt
    33601 tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca
    33661 tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt
    33721 acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt
    33781 acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa
    33841 ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg
    33901 tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact
    33961 tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa
    34021 caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt
    34081 gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag
    34141 taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat
    34201 tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt
    34261 cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc
    34321 cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa
    34381 ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat
    34441 ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc
    34501 gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata
    34561 tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac
    34621 cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt
    34681 atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa
    34741 aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg
    34801 caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc
    34861 aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag
    34921 cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc
    34981 ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg
    35041 cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg
    35101 ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg
    35161 gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg
    35221 cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc
    35281 cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg
    35341 cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag
    35401 acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag
    35461 ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga
    35521 atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca
    35581 aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg
    35641 atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag
    35701 aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg
    35761 atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga
    35821 aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga
    35881 taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa
    35941 tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat
    36001 tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga
    36061 aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa
    36121 attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga
    36181 tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc
    36241 ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca
    36301 attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga
    36361 agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt
    36421 aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt
    36481 tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg
    36541 ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac
    36601 agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa
    36661 agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg
    36721 tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg
    36781 attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac
    36841 agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc
    36901 tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa
    36961 attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg
    37021 atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc
    37081 tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaatt
    37141 aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct
    37201 aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta
    37261 cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc
    37321 cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt
    37381 gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt
    37441 aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt
    37501 tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg
    37561 cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc
    37621 ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg
    37681 acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc
    37741 actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt
    37801 tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac
    37861 tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat
    37921 ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg
    37981 cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc
    38041 gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt
    38101 tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga
    38161 tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa
    38221 agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga
    38281 aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact
    38341 gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga
    38401 aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa
    38461 ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta
    38521 gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat
    38581 gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct
    38641 acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg
    38701 aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg
    38761 ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga
    38821 aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc
    38881 tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa
    38941 gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca
    39001 ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt
    39061 atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg
    39121 agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc
    39181 cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg
    39241 tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg
    39301 atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg
    39361 cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa
    39421 atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt
    39481 tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg
    39541 tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc
    39601 cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta
    39661 tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag
    39721 tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc
    39781 tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga
    39841 tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg
    39901 ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa
    39961 aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta
    40021 gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg
    40081 cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc
    40141 tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca
    40201 gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg
    40261 gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg
    40321 gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg
    40381 tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt
    40441 acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg
    40501 aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta
    40561 cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca
    40621 tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag
    40681 gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt
    40741 tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca
    40801 tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt
    40861 cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga
    40921 ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat
    40981 tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct
    41041 tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat
    41101 tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga
    41161 tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg
    41221 atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg
    41281 ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa
    41341 gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt
    41401 tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt
    41461 tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt
    41521 cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc
    41581 acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg
    41641 tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat
    41701 agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat
    41761 tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat
    41821 gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa
    41881 agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga
    41941 cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga
    42001 actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa
    42061 aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa
    42121 tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat
    42181 ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt
    42241 gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga
    42301 cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca
    42361 agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa
    42421 aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc
    42481 agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat
    42541 atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac
    42601 attttttgag gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa
    42661 ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc
    42721 gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc
    42781 ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa
    42841 aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt
    42901 acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa
    42961 ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg
    43021 agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata
    43081 gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt
    43141 gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg
    43201 atccctggtg aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa
    43261 gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct
    43321 gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat
    43381 tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg
    43441 ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact
    43501 gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg
    43561 gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa
    43621 atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta
    43681 actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga
    43741 tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc
    43801 gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct
    43861 ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga
    43921 cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg
    43981 actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt
    44041 ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt
    44101 tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg
    44161 atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa
    44221 ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat
    44281 ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa
    44341 cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt
    44401 ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac
    44461 catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag
    44521 gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat
    44581 gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata
    44641 gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc
    44701 cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa
    44761 acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac
    44821 cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg
    44881 atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt
    44941 tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag
    45001 gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt
    45061 cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa
    45121 gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa
    45181 ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc
    45241 caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt
    45301 cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag
    45361 gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact
    45421 cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat
    45481 ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa
    45541 caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta
    45601 gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag aatagagctg
    45661 gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag
    45721 cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa
    45781 caattgcaag atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag
    45841 ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa
    45901 gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat
    45961 gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt
    46021 tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa
    46081 cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta
    46141 gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca acagttagaa
    46201 gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa
    46261 attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa
    46321 ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag
    46381 aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca
    46441 aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt
    46501 ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg
    46561 tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca
    46621 ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt
    46681 attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta
    46741 gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa
    46801 gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc
    46861 tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc
    46921 attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa
    46981 gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat
    47041 ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt
    47101 aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa
    47161 aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa
    47221 ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa
    47281 taccaagtag aaacgattga gactaatcag tatcacccag agctttctat tttggcactt
    47341 tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat
    47401 gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt
    47461 cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga
    47521 agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat
    47581 aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag
    47641 actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca attttccgat
    47701 caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca
    47761 ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt
    47821 tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga
    47881 ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt
    47941 atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta
    48001 gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct
    48061 cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg
    48121 acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta
    48181 ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg
    48241 gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc
    48301 agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc
    48361 gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag
    48421 caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa
    48481 aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa
    48541 caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga
    48601 ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg
    48661 atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat
    48721 gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat
    48781 cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc
    48841 tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg
    48901 agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa
    48961 gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga
    49021 gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat
    49081 tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca
    49141 ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat
    49201 ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca
    49261 aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg
    49321 cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct
    49381 gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa
    49441 atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg
    49501 acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt
    49561 tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga
    49621 gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag
    49681 ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg
    49741 gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga
    49801 actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct
    49861 gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa
    49921 gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa
    49981 aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact
    50041 gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa
    50101 agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa
    50161 aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt
    50221 ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag
    50281 aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa
    50341 gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt
    50401 ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt
    50461 ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt
    50521 tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc
    50581 ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc
    50641 agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa
    50701 tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta
    50761 gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga
    50821 tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc
    50881 gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca
    50941 aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca
    51001 gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct
    51061 tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat
    51121 aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc
    51181 tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac tattcaaaca
    51241 taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa tcattgcttt
    51301 aaaatactac gcttatataa cag
//
LOCUS       DACELF010000008        47113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.008, whole genome shotgun sequence.
ACCESSION   DACELF010000008 DACELF010000000
VERSION     DACELF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.008"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1528)
                     /locus_tag="IW687_000484"
     CDS             complement(137..1528)
                     /locus_tag="IW687_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0949774.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IW687_000485"
     CDS             complement(1741..2127)
                     /locus_tag="IW687_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0949775.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IW687_000486"
     CDS             complement(2183..2806)
                     /locus_tag="IW687_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0949776.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IW687_000487"
     CDS             complement(2870..3538)
                     /locus_tag="IW687_000487"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0949777.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IW687_000488"
     CDS             complement(3550..3990)
                     /locus_tag="IW687_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949778.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IW687_000489"
     CDS             complement(4049..4720)
                     /locus_tag="IW687_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0949779.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="IW687_000490"
     CDS             complement(4905..5303)
                     /locus_tag="IW687_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0949780.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="IW687_000491"
     CDS             complement(5300..6073)
                     /locus_tag="IW687_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0949781.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="IW687_000492"
     CDS             complement(6070..6753)
                     /locus_tag="IW687_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0949782.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="IW687_000493"
     CDS             complement(6920..7807)
                     /locus_tag="IW687_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0949783.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="IW687_000494"
     CDS             complement(7804..8673)
                     /locus_tag="IW687_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0949784.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="IW687_000495"
     CDS             complement(8810..10096)
                     /locus_tag="IW687_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0949785.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="IW687_000496"
     CDS             10369..10947
                     /locus_tag="IW687_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0949786.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="IW687_000497"
     CDS             10947..11876
                     /locus_tag="IW687_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0949787.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="IW687_000498"
     CDS             11873..13609
                     /locus_tag="IW687_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0949788.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IW687_000499"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IW687_000499"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0949789.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="IW687_000500"
     CDS             complement(14505..15014)
                     /locus_tag="IW687_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0949790.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IW687_000501"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IW687_000501"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0949791.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="IW687_000502"
     CDS             complement(16315..17406)
                     /locus_tag="IW687_000502"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0949792.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IW687_000503"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IW687_000503"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0949793.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IW687_000504"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IW687_000504"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0949794.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IW687_000505"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IW687_000505"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0949795.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="IW687_000506"
     CDS             complement(21369..22343)
                     /locus_tag="IW687_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0949796.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="IW687_000507"
     CDS             complement(22324..24207)
                     /locus_tag="IW687_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0949797.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="IW687_000508"
     CDS             complement(24225..26450)
                     /locus_tag="IW687_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0949798.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26565..27599
                     /locus_tag="IW687_000509"
     CDS             26565..27599
                     /locus_tag="IW687_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0949799.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27666..28679)
                     /locus_tag="IW687_000510"
     CDS             complement(27666..28679)
                     /locus_tag="IW687_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0949800.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28757..28945)
                     /locus_tag="IW687_000511"
     CDS             complement(28757..28945)
                     /locus_tag="IW687_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0949801.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29385..29513)
                     /locus_tag="IW687_000512"
     CDS             complement(29385..29513)
                     /locus_tag="IW687_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0949802.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="IW687_000513"
     CDS             complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="IW687_000513"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0949803.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31000..32055)
                     /locus_tag="IW687_000514"
     CDS             complement(31000..32055)
                     /locus_tag="IW687_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0949804.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALGEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="IW687_000515"
     CDS             complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="IW687_000515"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0949805.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="IW687_000516"
     CDS             complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="IW687_000516"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0949806.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="IW687_000517"
     CDS             complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="IW687_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0949807.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="IW687_000518"
     CDS             complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="IW687_000518"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0949808.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="IW687_000519"
     CDS             complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="IW687_000519"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0949809.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39817..41376
                     /locus_tag="IW687_000520"
     CDS             39817..41376
                     /locus_tag="IW687_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0949810.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41487..43418)
                     /locus_tag="IW687_000521"
     CDS             complement(41487..43418)
                     /locus_tag="IW687_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0949811.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="IW687_000522"
     CDS             complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="IW687_000522"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0949812.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44347..45099)
                     /locus_tag="IW687_000523"
     CDS             complement(44347..45099)
                     /locus_tag="IW687_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0949813.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45259..45468)
                     /locus_tag="IW687_000524"
     CDS             complement(45259..45468)
                     /locus_tag="IW687_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949814.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45479..46807)
                     /locus_tag="IW687_000525"
     CDS             complement(45479..46807)
                     /locus_tag="IW687_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949815.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13145 a        10183 c         8398 g        15387 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgctttttt tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa
    26521 ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag
    26581 atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc
    26641 agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg
    26701 attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct
    26761 ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga
    26821 tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata
    26881 atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta
    26941 cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc
    27001 ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg
    27061 tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag
    27121 tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct
    27181 atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct
    27241 ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc
    27301 ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac
    27361 aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa
    27421 tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag
    27481 cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga
    27541 cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa
    27601 agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca
    27661 acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct
    27721 tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac
    27781 aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc
    27841 tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg
    27901 tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac
    27961 gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct
    28021 gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga
    28081 agtagctgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat
    28141 tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc
    28201 aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact
    28261 tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg
    28321 gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat
    28381 cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag
    28441 atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg
    28501 tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata
    28561 tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac
    28621 gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata
    28681 aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca
    28741 agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa
    28801 gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac
    28861 attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt
    28921 caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca
    28981 actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg
    29041 tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc
    29101 atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt
    29161 gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg
    29221 aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca
    29281 actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga
    29341 aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt
    29401 ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg
    29461 cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt
    29521 ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag
    29581 gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag
    29641 ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa
    29701 taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc
    29761 ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt
    29821 cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca
    29881 tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat
    29941 aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca
    30001 gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt
    30061 ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta
    30121 aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt
    30181 cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta
    30241 aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc
    30301 cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta
    30361 ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat
    30421 catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga
    30481 tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa
    30541 cttctggcat cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa
    30601 gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt
    30661 tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa
    30721 acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga
    30781 taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga
    30841 tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct
    30901 ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa
    30961 accggcactg accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt
    31021 ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt
    31081 tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc
    31141 aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat
    31201 cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc
    31261 ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg
    31321 tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt
    31381 cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa
    31441 tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc
    31501 ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt
    31561 cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa
    31621 ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat
    31681 gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct
    31741 aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt
    31801 aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc
    31861 agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga
    31921 atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctc ctaatgcctg
    31981 taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac
    32041 gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt
    32101 cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc
    32161 aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat
    32221 ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc
    32281 aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat
    32341 ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt
    32401 caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat
    32461 caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc
    32521 agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg
    32581 gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat
    32641 ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact
    32701 agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt
    32761 ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc
    32821 taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa
    32881 ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc
    32941 cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc
    33001 gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc
    33061 ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc
    33121 ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc
    33181 gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt
    33241 atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa
    33301 tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc
    33361 tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg
    33421 cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac
    33481 tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct
    33541 gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg
    33601 atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga catacctgga
    33661 agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg
    33721 ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca
    33781 taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt
    33841 ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt
    33901 tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc
    33961 acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct
    34021 tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact
    34081 tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt
    34141 acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg
    34201 atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct
    34261 ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc
    34321 attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg
    34381 ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc
    34441 ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca
    34501 ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa
    34561 gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc
    34621 atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa
    34681 aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca
    34741 attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc
    34801 tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct
    34861 ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc
    34921 atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc
    34981 cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca
    35041 ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt
    35101 caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca
    35161 tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa
    35221 gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc
    35281 tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat
    35341 tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag
    35401 ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg
    35461 tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac
    35521 cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg
    35581 ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt
    35641 gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca
    35701 attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga
    35761 tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga
    35821 aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa
    35881 tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg
    35941 atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag
    36001 caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat
    36061 cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc
    36121 atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac
    36181 aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca
    36241 cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca
    36301 agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg
    36361 ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga
    36421 ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg
    36481 ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca
    36541 ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt
    36601 caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga
    36661 atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga
    36721 aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta
    36781 actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg
    36841 cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta
    36901 cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt
    36961 cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat
    37021 cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta
    37081 cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa
    37141 cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt
    37201 ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga
    37261 ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt
    37321 ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag
    37381 cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca
    37441 attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt
    37501 tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt
    37561 ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga
    37621 acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt
    37681 tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca
    37741 aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt
    37801 aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc
    37861 agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc
    37921 aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg
    37981 gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc
    38041 gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg
    38101 aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat
    38161 gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat
    38221 tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca
    38281 cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca
    38341 agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg
    38401 atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc
    38461 agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct
    38521 ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac acgagattgt
    38581 gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct
    38641 tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa
    38701 gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg
    38761 ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct
    38821 agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct
    38881 gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat
    38941 ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct
    39001 acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat
    39061 aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct
    39121 ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt
    39181 gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt
    39241 cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc
    39301 agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct
    39361 cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca
    39421 gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta
    39481 atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt
    39541 gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg
    39601 tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac
    39661 attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt
    39721 aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg
    39781 atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag
    39841 aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca
    39901 ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt
    39961 atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc
    40021 cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg
    40081 attccacaag tagagggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta
    40141 tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa
    40201 ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt
    40261 ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca
    40321 ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct
    40381 ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac
    40441 tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat
    40501 tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat
    40561 gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg
    40621 ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga
    40681 ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt
    40741 ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg
    40801 attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg
    40861 gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt
    40921 tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt
    40981 ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat
    41041 gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact
    41101 cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt
    41161 tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct
    41221 atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat
    41281 attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct
    41341 aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca
    41401 acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg
    41461 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
    41521 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
    41581 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
    41641 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
    41701 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
    41761 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
    41821 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
    41881 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
    41941 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
    42001 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
    42061 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
    42121 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
    42181 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
    42241 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
    42301 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
    42361 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
    42421 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
    42481 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
    42541 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
    42601 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
    42661 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
    42721 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
    42781 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
    42841 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
    42901 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
    42961 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
    43021 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
    43081 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
    43141 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
    43201 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
    43261 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
    43321 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
    43381 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
    43441 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
    43501 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
    43561 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
    43621 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
    43681 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
    43741 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
    43801 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
    43861 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
    43921 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
    43981 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
    44041 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
    44101 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
    44161 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
    44221 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
    44281 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
    44341 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
    44401 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
    44461 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
    44521 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
    44581 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
    44641 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
    44701 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
    44761 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
    44821 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
    44881 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
    44941 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
    45001 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
    45061 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
    45121 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
    45181 tataaatcag tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
    45241 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
    45301 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
    45361 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
    45421 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
    45481 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
    45541 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
    45601 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
    45661 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
    45721 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
    45781 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
    45841 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
    45901 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacgggt tgcatttgtg
    45961 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
    46021 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
    46081 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
    46141 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
    46201 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
    46261 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
    46321 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
    46381 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
    46441 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
    46501 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
    46561 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
    46621 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
    46681 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
    46741 gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
    46801 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
    46861 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
    46921 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
    46981 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
    47041 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
    47101 taagctcaaa aaa
//
LOCUS       DACELF010000009        45189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.009, whole genome shotgun sequence.
ACCESSION   DACELF010000009 DACELF010000000
VERSION     DACELF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.009"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..2095)
                     /locus_tag="IW687_000526"
     CDS             complement(23..2095)
                     /locus_tag="IW687_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0949816.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IW687_000527"
     CDS             complement(2028..2405)
                     /locus_tag="IW687_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0949817.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IW687_000528"
     CDS             complement(2534..3094)
                     /locus_tag="IW687_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0949818.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IW687_000529"
     CDS             3244..4536
                     /locus_tag="IW687_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0949819.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IW687_000530"
     CDS             complement(4596..5714)
                     /locus_tag="IW687_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0949820.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW687_000531"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW687_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0949821.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW687_000532"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW687_000532"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0949822.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IW687_000533"
     CDS             8920..9519
                     /locus_tag="IW687_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0949823.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW687_000534"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW687_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0949824.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IW687_000535"
     CDS             complement(11214..11558)
                     /locus_tag="IW687_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0949825.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IW687_000536"
     CDS             complement(11695..12831)
                     /locus_tag="IW687_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0949826.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IW687_000537"
     CDS             complement(13020..13994)
                     /locus_tag="IW687_000537"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0949827.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IW687_000538"
     CDS             complement(14074..14949)
                     /locus_tag="IW687_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0949828.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IW687_000539"
     CDS             15079..16986
                     /locus_tag="IW687_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0949829.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IW687_000540"
     CDS             complement(17527..20805)
                     /locus_tag="IW687_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0949830.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IW687_000541"
     CDS             complement(21076..21621)
                     /locus_tag="IW687_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0949831.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IW687_000542"
     CDS             complement(21633..22325)
                     /locus_tag="IW687_000542"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0949832.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IW687_000543"
     CDS             complement(22344..22628)
                     /locus_tag="IW687_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949833.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IW687_000544"
     CDS             complement(22641..23204)
                     /locus_tag="IW687_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0949834.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IW687_000545"
     CDS             23432..24094
                     /locus_tag="IW687_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0949835.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IW687_000546"
     CDS             24218..25414
                     /locus_tag="IW687_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0949836.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIAFTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IW687_000547"
     CDS             complement(25515..26891)
                     /locus_tag="IW687_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0949837.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IW687_000548"
     CDS             complement(26928..27794)
                     /locus_tag="IW687_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0949838.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IW687_000549"
     CDS             complement(27883..28908)
                     /locus_tag="IW687_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0949839.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IW687_000550"
     CDS             complement(28926..29600)
                     /locus_tag="IW687_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949840.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IW687_000551"
     CDS             complement(29784..30677)
                     /locus_tag="IW687_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0949841.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IW687_000552"
     CDS             complement(30751..32448)
                     /locus_tag="IW687_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0949842.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IW687_000553"
     CDS             complement(32441..33754)
                     /locus_tag="IW687_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0949843.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IW687_000554"
     CDS             complement(33754..37272)
                     /locus_tag="IW687_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0949844.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IW687_000555"
     CDS             complement(37565..38023)
                     /locus_tag="IW687_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0949845.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38321..38839)
                     /locus_tag="IW687_000556"
     CDS             complement(38321..38839)
                     /locus_tag="IW687_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0949846.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38841..39458)
                     /locus_tag="IW687_000557"
     CDS             complement(38841..39458)
                     /locus_tag="IW687_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0949847.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IW687_000558"
     CDS             complement(39455..40309)
                     /locus_tag="IW687_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0949848.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IW687_000559"
     CDS             complement(40306..41217)
                     /locus_tag="IW687_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0949849.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IW687_000560"
     CDS             complement(41258..42592)
                     /locus_tag="IW687_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0949850.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IW687_000561"
     CDS             42800..43630
                     /locus_tag="IW687_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0949851.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IW687_000562"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IW687_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IW687_000563"
     CDS             complement(44314..44508)
                     /locus_tag="IW687_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949852.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        13071 a         9721 c         7969 g        14428 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg ttaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta tcattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttccttttct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc atttaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ttgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg atagtcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tagaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaatt
//
LOCUS       DACELF010000010        44673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.010, whole genome shotgun sequence.
ACCESSION   DACELF010000010 DACELF010000000
VERSION     DACELF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.010"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(280..1077)
                     /locus_tag="IW687_000564"
     CDS             complement(280..1077)
                     /locus_tag="IW687_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0949853.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1074..2258)
                     /locus_tag="IW687_000565"
     CDS             complement(1074..2258)
                     /locus_tag="IW687_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0949854.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2283..3530)
                     /locus_tag="IW687_000566"
     CDS             complement(2283..3530)
                     /locus_tag="IW687_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0949855.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3745..5490
                     /gene="dnaX"
                     /locus_tag="IW687_000567"
     CDS             3745..5490
                     /gene="dnaX"
                     /locus_tag="IW687_000567"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0949856.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5510..5824
                     /locus_tag="IW687_000568"
     CDS             5510..5824
                     /locus_tag="IW687_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0949857.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5849..6445
                     /gene="recR"
                     /locus_tag="IW687_000569"
     CDS             5849..6445
                     /gene="recR"
                     /locus_tag="IW687_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0949858.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6509..7153
                     /locus_tag="IW687_000570"
     CDS             6509..7153
                     /locus_tag="IW687_000570"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0949859.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7168..7497
                     /locus_tag="IW687_000571"
     CDS             7168..7497
                     /locus_tag="IW687_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949860.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7511..8449
                     /gene="holB"
                     /locus_tag="IW687_000572"
     CDS             7511..8449
                     /gene="holB"
                     /locus_tag="IW687_000572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0949861.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8485..9309
                     /locus_tag="IW687_000573"
     CDS             8485..9309
                     /locus_tag="IW687_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0949862.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9302..9649
                     /locus_tag="IW687_000574"
     CDS             9302..9649
                     /locus_tag="IW687_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0949863.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9718..10590
                     /gene="rsmI"
                     /locus_tag="IW687_000575"
     CDS             9718..10590
                     /gene="rsmI"
                     /locus_tag="IW687_000575"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0949864.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10699..11820
                     /gene="dinB"
                     /locus_tag="IW687_000576"
     CDS             10699..11820
                     /gene="dinB"
                     /locus_tag="IW687_000576"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0949865.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11874..12476)
                     /gene="nrdG"
                     /locus_tag="IW687_000577"
     CDS             complement(11874..12476)
                     /gene="nrdG"
                     /locus_tag="IW687_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0949866.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12791..14980)
                     /gene="nrdD"
                     /locus_tag="IW687_000578"
     CDS             complement(12791..14980)
                     /gene="nrdD"
                     /locus_tag="IW687_000578"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0949867.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15306..16073
                     /locus_tag="IW687_000579"
     CDS             15306..16073
                     /locus_tag="IW687_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949868.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16063..18105
                     /locus_tag="IW687_000580"
     CDS             16063..18105
                     /locus_tag="IW687_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949869.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18386..18535
                     /locus_tag="IW687_000581"
     CDS             18386..18535
                     /locus_tag="IW687_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0949870.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18546..20060
                     /gene="dltA"
                     /locus_tag="IW687_000582"
     CDS             18546..20060
                     /gene="dltA"
                     /locus_tag="IW687_000582"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0949871.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20057..21262
                     /gene="dltB"
                     /locus_tag="IW687_000583"
     CDS             20057..21262
                     /gene="dltB"
                     /locus_tag="IW687_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0949872.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21307..21540
                     /gene="dltC"
                     /locus_tag="IW687_000584"
     CDS             21307..21540
                     /gene="dltC"
                     /locus_tag="IW687_000584"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0949873.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21543..22811
                     /gene="dltD"
                     /locus_tag="IW687_000585"
     CDS             21543..22811
                     /gene="dltD"
                     /locus_tag="IW687_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0949874.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22952..24034
                     /locus_tag="IW687_000586"
     CDS             22952..24034
                     /locus_tag="IW687_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0949875.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24034..24837
                     /locus_tag="IW687_000587"
     CDS             24034..24837
                     /locus_tag="IW687_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0949876.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGVNLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24907..25647
                     /locus_tag="IW687_000588"
     CDS             24907..25647
                     /locus_tag="IW687_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0949877.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25658..26662
                     /locus_tag="IW687_000589"
     CDS             25658..26662
                     /locus_tag="IW687_000589"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0949878.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26707..28551)
                     /locus_tag="IW687_000590"
     CDS             complement(26707..28551)
                     /locus_tag="IW687_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949879.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28763..29281
                     /locus_tag="IW687_000591"
     CDS             28763..29281
                     /locus_tag="IW687_000591"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0949880.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29409..30092
                     /locus_tag="IW687_000592"
     CDS             29409..30092
                     /locus_tag="IW687_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0949881.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30401..30474
                     /locus_tag="IW687_000593"
     tRNA            30401..30474
                     /locus_tag="IW687_000593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30435..30437,aa:Arg,seq:tct)
     gene            30633..30992
                     /locus_tag="IW687_000594"
     CDS             30633..30992
                     /locus_tag="IW687_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0949882.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31115..32746
                     /locus_tag="IW687_000595"
     CDS             31115..32746
                     /locus_tag="IW687_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0949883.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32808..33449)
                     /locus_tag="IW687_000596"
     CDS             complement(32808..33449)
                     /locus_tag="IW687_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0949884.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33731..34039
                     /gene="rplU"
                     /locus_tag="IW687_000597"
     CDS             33731..34039
                     /gene="rplU"
                     /locus_tag="IW687_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0949885.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34066..34410
                     /locus_tag="IW687_000598"
     CDS             34066..34410
                     /locus_tag="IW687_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0949886.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34423..34716
                     /gene="rpmA"
                     /locus_tag="IW687_000599"
     CDS             34423..34716
                     /gene="rpmA"
                     /locus_tag="IW687_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0949887.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35123..37957
                     /locus_tag="IW687_000600"
     CDS             35123..37957
                     /locus_tag="IW687_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0949888.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38148..38639
                     /locus_tag="IW687_000601"
     CDS             38148..38639
                     /locus_tag="IW687_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0949889.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38742..39731
                     /gene="manX"
                     /locus_tag="IW687_000602"
     CDS             38742..39731
                     /gene="manX"
                     /locus_tag="IW687_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0949890.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39764..40567
                     /locus_tag="IW687_000603"
     CDS             39764..40567
                     /locus_tag="IW687_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0949891.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40588..41502
                     /locus_tag="IW687_000604"
     CDS             40588..41502
                     /locus_tag="IW687_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0949892.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41585..41959
                     /locus_tag="IW687_000605"
     CDS             41585..41959
                     /locus_tag="IW687_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0949893.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42098..42343
                     /locus_tag="IW687_000606"
     CDS             42098..42343
                     /locus_tag="IW687_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0949894.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42515..43579
                     /locus_tag="IW687_000607"
     CDS             42515..43579
                     /locus_tag="IW687_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0949895.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43825..44250
                     /locus_tag="IW687_000608"
     CDS             43825..44250
                     /locus_tag="IW687_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0949896.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44243..>44673
                     /gene="nusB"
                     /locus_tag="IW687_000609"
     CDS             44243..>44673
                     /gene="nusB"
                     /locus_tag="IW687_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0949897.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLK"
BASE COUNT        14189 a         7697 c         9379 g        13408 t
ORIGIN      
        1 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
       61 ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt
      121 aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg acttatcacc
      181 gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga cacgattttt
      241 gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc tgccgccacc
      301 gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc ttccaggagg
      361 tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat cggttttttc
      421 agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg atttcaattg
      481 ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca aaacaacagc
      541 aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag tttcttcgtc
      601 tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt tagacaggta
      661 agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat catctaagat
      721 cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg aaatgtagat
      781 ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc cgactgcgtc
      841 tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag tcgttacgat
      901 caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca aagattggat
      961 ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg catgagtagc
     1021 caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt tcatcatatc
     1081 aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag agcggtcagt
     1141 cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg tccattcatt
     1201 gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag ccataccggc
     1261 agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac accggtcaat
     1321 gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt taattcagat
     1381 tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg gaacccggct
     1441 aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac gccttcgacc
     1501 atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa agaaagcttg
     1561 cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat
     1621 atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa
     1681 taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa agctttggga
     1741 acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc tttctcgaca
     1801 gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga tagtacgacg
     1861 ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac gatctctgca
     1921 ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga ttcgtccgct
     1981 gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt catatcggca
     2041 agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc tttgctttct
     2101 tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc ttcttctgta
     2161 tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga
     2221 ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc tttctaaata
     2281 aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa tttagggatg
     2341 ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag cttaggctga
     2401 acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga attttctgtc
     2461 ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg attttgtttt
     2521 tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag ataagaacca
     2581 tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc ttcatttttt
     2641 ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg tacgctagga
     2701 tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc atcggtgtaa
     2761 gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg ccaagaatca
     2821 tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac ataagaaatc
     2881 cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag gaattcagct
     2941 aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg gattgcgttt
     3001 gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc ccgtaagaat
     3061 aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt
     3121 ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt gacgccgcca
     3181 aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt gaacatggaa
     3241 ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc gatgattttt
     3301 ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt tccgtctgtt
     3361 aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg gactttgaca
     3421 ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc tagccattga
     3481 tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat aaaaaattcc
     3541 tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat aggaagatgt
     3601 gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt taaatacctg
     3661 aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa gagatagtga
     3721 attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt ctggcgttcg
     3781 cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa aaatgctatt
     3841 gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac cggaaagacg
     3901 agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga cggtgagcca
     3961 tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa
     4021 atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat
     4081 tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca tatgctttca
     4141 acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag tgtcatattc
     4201 atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg gactcagcga
     4261 tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt tatcttgaaa
     4321 gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc tgcagaaggc
     4381 gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga aggaacaatt
     4441 actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat ggacagttac
     4501 ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa ccaaattctc
     4561 gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg ccgtgacttg
     4621 ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat cagaagtaca
     4681 tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat aaagattttg
     4741 agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt ggaagtcgct
     4801 acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt agcatttgca
     4861 gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa cgaaatcaag
     4921 gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca gcaaacaaat
     4981 cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa agttctgcgg
     5041 gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt gctgatgagt
     5101 ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc tggacctaca
     5161 ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa
     5221 ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc gcctgatcct
     5281 gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac gcaggcacaa
     5341 gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat cgagttgctt
     5401 cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga actgtttggc
     5461 tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta tgatgcgtgg
     5521 aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga
     5581 agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg aattggtcaa
     5641 agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag aagtcgttga
     5701 tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc
     5761 acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt
     5821 ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg cgaaactgat
     5881 cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac tcgcctttta
     5941 tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa
     6001 acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat
     6061 atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt
     6121 aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc atggtgtgtt
     6181 atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt tgcagcggct
     6241 gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag agggagaagc
     6301 aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc
     6361 acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt tattgaaagc
     6421 tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga tacaatataa
     6481 aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa tcgaggggcc
     6541 tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga aagaagtagc
     6601 aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg aagagatccg
     6661 agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag cactgcttta
     6721 tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg caaaagggaa
     6781 aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg caggccgacg
     6841 catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta ctacgccgga
     6901 ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca agaaaaacag
     6961 acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc gtgtacgtca
     7021 tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa
     7081 aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt atccgcaatt
     7141 ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat ccaagacaaa
     7201 gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac aaaactatca
     7261 tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat tgaagatgac
     7321 cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta
     7381 tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc agtagaagta
     7441 gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca attttaggag
     7501 gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg tgtatcagca
     7561 gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg aaggggagaa
     7621 aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct gtacccaaat
     7681 gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca
     7741 tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc agatcagacg
     7801 tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa
     7861 agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct tggaagagcc
     7921 ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc tccctactat
     7981 tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac tgatccatcg
     8041 attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct taacaaacag
     8101 tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta aggacttgat
     8161 ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct atgtccaaaa
     8221 aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat ctgtcttgat
     8281 gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa
     8341 acagataaac cagataattg aacggatatt gatcgcagag cagaaactac gcagcaatgt
     8401 cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc ttgccgcaaa
     8461 ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata tagagatgca
     8521 ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga caaggtatta
     8581 gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa agagattgat
     8641 cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca agctgaatta
     8701 gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa
     8761 gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat
     8821 aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt ggtaaaagat
     8881 ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca
     8941 aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc ttttttaggc
     9001 gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt gaatccaaca
     9061 aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag cgaagaatac
     9121 gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa
     9181 gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct gactggacgg
     9241 gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt aggtgagatg
     9301 aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt tgagaaatac
     9361 attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac
     9421 tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc aagttgcaca
     9481 agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat
     9541 ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg acaatgacga
     9601 gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac caataacaag
     9661 aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt gtgacagatg
     9721 tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct tgtaccgacg
     9781 ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca agaatccgat
     9841 cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt tgaaatccat
     9901 acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca attgattgct
     9961 ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc ttccatcagc
    10021 gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt gatctctatc
    10081 ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc tcaaccattt
    10141 ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta
    10201 aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac tgctacattg
    10261 acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga attgacaaag
    10321 gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat cgaagaacat
    10381 ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat gccagtgacg
    10441 gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat cgcttcaggc
    10501 gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa
    10561 gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata atgaaagact
    10621 aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat gttcttgtat
    10681 aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga tacatcaaga
    10741 aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct
    10801 gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg tgggcgtgga
    10861 gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc aatgagtgca
    10921 caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca tcataaatac
    10981 agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat tgtggaacca
    11041 gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg caagtcagcc
    11101 atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct aacatgttct
    11161 gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca aaaacctaga
    11221 ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt gccaattgaa
    11281 aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg gatttataca
    11341 ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg aaaaatgggc
    11401 tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt tacaagagag
    11461 cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga agaagcagtg
    11521 ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag agtccaaaaa
    11581 catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt gaccaaaaga
    11641 gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc aagccttata
    11701 tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac tttgacgaat
    11761 ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca agaaatataa
    11821 aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg tttttatatc
    11881 aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt ttcccaaatc
    11941 acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt gctacttccg
    12001 cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac aaggatatcg
    12061 atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc aaaggtatag
    12121 cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg tcgtacgact
    12181 tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg
    12241 taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa tggtttgcca
    12301 tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa gcagccacta
    12361 acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt gtagtcagca
    12421 atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt tctcataaaa
    12481 aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct actcgaccac
    12541 ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct tattttgata
    12601 gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac ttgtcatagc
    12661 ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag cccaactaaa
    12721 cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc aggacagctt
    12781 tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg cggccatgta
    12841 ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact acatcacatg
    12901 tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc ggtttgaaat
    12961 caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca agatacccga
    13021 ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt tggagcatag
    13081 ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc ttctcaaatt
    13141 ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg tttgtataat
    13201 attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac
    13261 ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca tattcatccc
    13321 cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa gcttttgctt
    13381 ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac aatccaatgt
    13441 agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa tcggtacgtg
    13501 aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct ggaatggctt
    13561 ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag cggtcagaca
    13621 gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt ggcaaattca
    13681 atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt tcgtctttcc
    13741 acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt aattcaacga
    13801 ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc aatgctagtt
    13861 gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc aaagtgaaga
    13921 tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt cgaatctgta
    13981 aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat cctaatgaag
    14041 tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc aaactttgca
    14101 tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg cgttctggcg
    14161 aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct gcaaaaggag
    14221 caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac gctacattgg
    14281 caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat tcgacttcgg
    14341 cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag cagcaattcg
    14401 tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct ttttgatgcg
    14461 catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg atcccggctg
    14521 tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg ttcacgacag
    14581 tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct ttactgcgag
    14641 caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat tcgttttttt
    14701 ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt tcagcaaacc
    14761 ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca taagtaagtg
    14821 gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg atcttttgtt
    14881 catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat gtctctggct
    14941 gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac tcctgctaaa
    15001 ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc ggctatatat
    15061 agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata ttttattgtg
    15121 aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat tttgctgaat
    15181 aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa cataagagca
    15241 aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag
    15301 aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc agcaaacttt
    15361 ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag tttgttggga
    15421 ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt
    15481 tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg
    15541 agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc aatttgatcg
    15601 ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg atgactaaag
    15661 aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa gaactgctca
    15721 aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct gctagagcac
    15781 ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg gactcgaagt
    15841 cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac
    15901 tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta tttattaaag
    15961 atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc tttgagaaag
    16021 taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta taaactagcc
    16081 gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag tttgacttta
    16141 tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt agtcagaaga
    16201 gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat cattactgtt
    16261 gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag gcaaaaggaa
    16321 ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat atatgtgtta
    16381 gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg tattattttt
    16441 tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac tagtccattt
    16501 tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat ccttttagca
    16561 gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag ttttggagaa
    16621 aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc
    16681 atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag ggaaatcact
    16741 tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc ttttttaatt
    16801 tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg gttatttttg
    16861 atcatattga gtaaatggaa gatttattac tatggtatca attttttgat aatgggtaac
    16921 acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt cattctcgga
    16981 atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc ctaccaggag
    17041 aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa aatcaagccg
    17101 attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta taaagtcatt
    17161 gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa tctgatcacg
    17221 gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat ttctttgaaa
    17281 tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa tatttcatta
    17341 tatggatcta gtattggact gccagataat caagaattga agattcaaca aatgcttcca
    17401 agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt agatgatacc
    17461 gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt atcaggtgga
    17521 aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg gctcaatgct
    17581 gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag cacaaagaaa
    17641 cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg tttgaatttc
    17701 tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc
    17761 acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac agtccggcaa
    17821 ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct ggggattcca
    17881 caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt tccacccatg
    17941 atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta tataaaaact
    18001 gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta tcttttgctt
    18061 tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac ggaataagac
    18121 aagataataa atagaactaa attttgaata ccatttattt ggaataagag gttgtgacac
    18181 gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa attataaaga
    18241 aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac tgttagtaaa
    18301 aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt acactttgtg
    18361 tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa aattgaatta
    18421 ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt tcctgattta
    18481 tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat tttaggagga
    18541 agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata aatgaacctg
    18601 atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa caagcatcag
    18661 acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt gtcgtttttg
    18721 gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct ggccatgcct
    18781 atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt cgggtggcta
    18841 aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg gttcctgtca
    18901 taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat cccagaaata
    18961 gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact ggagaaccaa
    19021 aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg atggaagact
    19081 ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt gatttatcgg
    19141 tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta aataaagcag
    19201 ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg aatgtgtggg
    19261 tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat ggagaaaatg
    19321 tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa actgcagaaa
    19381 gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca acagaagcaa
    19441 ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat gaccgactac
    19501 cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt aaacctgttt
    19561 caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa ggttacctga
    19621 acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca agttaccata
    19681 caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga cgtatggatc
    19741 agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat tatctgctcc
    19801 aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca aaagtacagc
    19861 agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt cagttgacta
    19921 aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct cagaagttcc
    19981 attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa aaattaacag
    20041 ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc cgttttattt
    20101 ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat gggaaaaaag
    20161 attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct ttggaggtaa
    20221 ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga tacttgtatg
    20281 gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg ccgccgttat
    20341 tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac atggaaaagc
    20401 atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac aaatgatcat
    20461 ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc agtttctgct
    20521 gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg aaaaagatca
    20581 gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg tccaaaatat
    20641 ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag tactgcttcc
    20701 tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag taggttatat
    20761 gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat ttgcagtcgg
    20821 tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt ttttatcatg
    20881 gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt tccgtgatta
    20941 tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa gcaagatcgt
    21001 aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc atggattgac
    21061 gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga cagatgcatg
    21121 gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta cgcatggctt
    21181 agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt caggattttt
    21241 agataaactt ttttttagct aacgaatagt gacatattaa tttatataac aaaggagaaa
    21301 aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac agatgaagta
    21361 agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt agcatctgtt
    21421 caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc tgaattcgag
    21481 cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt gaagggttga
    21541 gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt ggcttactgt
    21601 ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg attagacgtg
    21661 cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat tatgcattat
    21721 ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc agtccatttc
    21781 atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg ctgggagcac
    21841 ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat gctatgaaaa
    21901 acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt gtaaaaactg
    21961 attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt tcaatgaaga
    22021 aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca aaagtaaaag
    22081 agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta cctttacctg
    22141 aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat gaagtattca
    22201 gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa cgattaccaa
    22261 aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa cgagaaacga
    22321 cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga gcccacgagc
    22381 cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa ttttctgatt
    22441 ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc atcatcccac
    22501 cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta caaggatttg
    22561 ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct gattttgtca
    22621 atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg aaaggctggt
    22681 tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact gctagtaagt
    22741 atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca gataaactac
    22801 aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc
    22861 tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa tggtataatc
    22921 acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac agctacttat
    22981 tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag aagtattcag
    23041 agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca gtgtgattgc
    23101 caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg acgaagttgg
    23161 ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc ttggaggttg
    23221 gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt
    23281 ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa gaaataaacc
    23341 ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag aagccaaaga
    23401 gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac gaatgaatgg
    23461 cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg tttctctgcg
    23521 agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt
    23581 gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa atcccgatat
    23641 tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac caaaagaagc
    23701 agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta tgatcccgca
    23761 taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc cattccaata
    23821 tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa gagatggtgt
    23881 tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat cagtgattca
    23941 tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac ggcttgatag
    24001 tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc tatttctgaa
    24061 tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac ttggcttgtt
    24121 ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt cagtcctttt
    24181 ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt acttttattc
    24241 gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg cagaccaaaa
    24301 agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact tggcgtcaat
    24361 ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc catgttttta
    24421 atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt acctacgggt
    24481 gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg gatgattttt
    24541 gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc atggacaaag
    24601 ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt tttattgaaa
    24661 gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta tttgctagga
    24721 gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca
    24781 gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt tacttagcat
    24841 gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt tgagggggaa
    24901 tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa aaatcaaggg
    24961 acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc aggtagtgct
    25021 acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt gatccttagc
    25081 cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg gcaacttcat
    25141 cctaagagaa atccggagac attgctttca atttatggac acacaaaaga ttccattcac
    25201 tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga agctgaagaa
    25261 ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt agtatgttac
    25321 gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg tgattcaggt
    25381 tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc agatacctat
    25441 ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc tggagaactt
    25501 gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt tggtgattta
    25561 gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat agaacttgct
    25621 gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac caaatgaaaa
    25681 ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa tgcctttaga
    25741 tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca gaaaccaaaa
    25801 tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg
    25861 cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta gttttatcat
    25921 gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa tcatccaagc
    25981 acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc tagtcattga
    26041 tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg
    26101 aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag taaaaaaaga
    26161 caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt
    26221 tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa
    26281 aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg atgaagattg
    26341 gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga attacgggaa
    26401 gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga ttgaagtcca
    26461 tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt tcttcggact
    26521 aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata aagcatctct
    26581 atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga
    26641 attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata tggattccgc
    26701 tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc gatcaatgaa
    26761 agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat
    26821 taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa ataggtttgt
    26881 atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga
    26941 caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa aggaatgctc
    27001 taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt cttcgggaag
    27061 atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt aacgcgctaa
    27121 gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat tttttgcttc
    27181 ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca ttaaatcttg
    27241 cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt ggatatgctg
    27301 tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga aatctacttg
    27361 tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga taataacgat
    27421 tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga tacctaatgc
    27481 cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag gtgtacaagc
    27541 tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag tgactaaagt
    27601 gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg caggagtaaa
    27661 agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa aaattccgta
    27721 ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag ctgacgttaa
    27781 gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa
    27841 aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta agagaataag
    27901 tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa aggtccctat
    27961 agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg aataaataaa
    28021 actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag catagttctg
    28081 ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg gtaaatctat
    28141 cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt ggtataaaat
    28201 cttttcgcct agatatacag taatccattg atggtttcct ttgattagta aatttgcttc
    28261 gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat ccacggtagg
    28321 aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg tatagaaaac
    28381 ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa
    28441 gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc tccactttat
    28501 acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca taaaggtatt
    28561 cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca attatttata
    28621 ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat taaagggaaa
    28681 catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa gaaggattga
    28741 ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta taagttctat
    28801 aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc gaatttttgg
    28861 tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac aaggaaatct
    28921 gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta ttacatatta
    28981 tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa acaaccacga
    29041 agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag taaaaaagag
    29101 aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc tttcaaaaac
    29161 ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa caggaatcac
    29221 agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga aatttttgta
    29281 agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa catgaaacac
    29341 gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt gtgaagagga
    29401 ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa ctattggatt
    29461 ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa gaagaagctc
    29521 atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa tttttacttg
    29581 gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt tcatcaagtg
    29641 tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt ttcgatgtca
    29701 tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa gtgactttgc
    29761 ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac tttattttta
    29821 tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt ttgttaagaa
    29881 aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg ctcccagatg
    29941 aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt cttcgtgttg
    30001 tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt gtgatcttaa
    30061 tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc tgtagataat
    30121 cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt gtaagtcaat
    30181 taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta gctcctgttt
    30241 tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga ttgatttagt
    30301 agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat tgacagaagc
    30361 aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag ctcagctgga
    30421 tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt gacgctaaaa
    30481 aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca atattgtttg
    30541 ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata aggagcaaaa
    30601 aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga attttagtga
    30661 ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga actgattctg
    30721 acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa tctgttcaaa
    30781 cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta ctttatggag
    30841 cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct attttttttg
    30901 ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa ggtgggctag
    30961 ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa tttatctaac
    31021 aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta atgaatggta
    31081 ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg tttcctcact
    31141 aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt tggctgggat
    31201 cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa gcggagctga
    31261 tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg gtttatcagg
    31321 cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga tgtctattgc
    31381 ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc tatttttact
    31441 tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac ccgtaattac
    31501 ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata atttatttgg
    31561 tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa cgataggatt
    31621 tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga tgtttatcta
    31681 tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt tggcaacagc
    31741 agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac aaacattgat
    31801 cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac aagtggcagt
    31861 tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg gtgcttcggc
    31921 tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc aaggaattgg
    31981 aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg cccgtacaag
    32041 cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg cagttgtctt
    32101 actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc cagcacttgc
    32161 aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca aggagctatt
    32221 tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt gtacagttat
    32281 ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt
    32341 caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa tggggctgcc
    32401 agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac tgttttttat
    32461 gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg gtgtcatttt
    32521 ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg atgaatttca
    32581 agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc cagaagtaga
    32641 aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa cagttgctca
    32701 tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca atgaaaaaga
    32761 acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta atattgctgt
    32821 aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga gcgattgtag
    32881 aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga ccattcgtag
    32941 gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc atgatttttg
    33001 gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt
    33061 aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg
    33121 gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg tttattgatc
    33181 cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac ctcttctcgt
    33241 gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc gctaaaagga
    33301 acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag tttaggatga
    33361 agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct taaagaagct
    33421 tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt tattataaga
    33481 ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag aaaagcagaa
    33541 gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta acggttgctg
    33601 ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc tcagaacaag
    33661 taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc agaaggaggt
    33721 gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg
    33781 acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg tttttgacga
    33841 agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg caggtgcaac
    33901 tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa
    33961 acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat
    34021 cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat caaaggaaca
    34081 tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca tgctgatgct
    34141 ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc
    34201 aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga agaagaaggc
    34261 ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa tattgctgag
    34321 attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa ctcagaatat
    34381 atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt atctatgaat
    34441 ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg tgactcagaa
    34501 tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg atcaattctt
    34561 taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg tggagacgac
    34621 actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa
    34681 caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat taaagaggtt
    34741 gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt agtggaggct
    34801 acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt
    34861 tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt
    34921 atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg
    34981 ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa
    35041 atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt
    35101 ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa
    35161 agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat
    35221 tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg
    35281 cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt
    35341 tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga
    35401 agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa
    35461 cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg
    35521 attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat
    35581 gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa
    35641 ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa
    35701 aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg
    35761 tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta
    35821 ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa
    35881 tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt
    35941 tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa
    36001 agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc
    36061 tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca
    36121 attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc
    36181 cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt
    36241 agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt
    36301 agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct
    36361 atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc
    36421 aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca
    36481 tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga
    36541 agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa
    36601 ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga
    36661 agaacggcat acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc
    36721 tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag
    36781 tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag
    36841 aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt
    36901 ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc
    36961 gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt
    37021 catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac
    37081 tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg
    37141 aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg
    37201 aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa
    37261 aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt
    37321 gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat
    37381 tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac
    37441 tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca
    37501 aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt
    37561 catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt
    37621 catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc
    37681 tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac
    37741 agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt
    37801 gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga
    37861 agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca
    37921 taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg atacactttt
    37981 atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa
    38041 aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga
    38101 aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat
    38161 tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa
    38221 caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt
    38281 ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa
    38341 tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca
    38401 atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg
    38461 gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac
    38521 aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag
    38581 tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc
    38641 aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat
    38701 ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag
    38761 caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac
    38821 aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca
    38881 aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt
    38941 tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat
    39001 gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt
    39061 caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa
    39121 aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac
    39181 aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat
    39241 tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag
    39301 tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga
    39361 gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa
    39421 tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa
    39481 atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg
    39541 ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag
    39601 aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag
    39661 tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag
    39721 caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta ttatttcaat
    39781 aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg atgaatttca
    39841 atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta acctcgaagc
    39901 tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa atatcggtgc
    39961 cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt tagttttagg
    40021 tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt tggctgttgc
    40081 agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga
    40141 tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca ttgctgtatg
    40201 tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc cagcacaaac
    40261 tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg ctatcggtgg
    40321 gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta caaaagaagt
    40381 atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa cattgattgc
    40441 tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta aaatgggtgg
    40501 ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca ttttgaatga
    40561 ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa
    40621 gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa ctacgaacgt
    40681 atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt atacaaaaca
    40741 aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac acacccatac
    40801 attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc gaatggcgcg
    40861 cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc tttagctggt
    40921 gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact aggtgcttca
    40981 ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg gaacattatt
    41041 cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc taaaattact
    41101 gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat tttagggatg
    41161 ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc agttgtatca
    41221 actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc aggtggtgaa
    41281 ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc tgaaaaggta
    41341 acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt aggattaaca
    41401 ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat ccttggttta
    41461 ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa aatatagctc
    41521 gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata tctggaggga
    41581 gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta cttcgtttac
    41641 gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt tttaccatgc
    41701 gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg tactagcatc
    41761 agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa aaaatggtac
    41821 atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga
    41881 tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg gtattcgtag
    41941 tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt ctttattttt
    42001 ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct ttaaatccca
    42061 tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat ggacattgat
    42121 tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa attcgatggg
    42181 ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag cgattttcgg
    42241 cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag gcgcagtgat
    42301 tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta caaaatagta
    42361 aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat ttaaaataaa
    42421 aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt tgctataatg
    42481 gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag aaaaattgcg
    42541 caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt acaatcttcg
    42601 ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg aaaaagcatt
    42661 ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag ggtttgaaat
    42721 tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca aagaagaact
    42781 gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg ttttagaaga
    42841 aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca gagaagtgaa
    42901 agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata tggcatatga
    42961 tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa atcaactaga
    43021 cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg
    43081 attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt
    43141 gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga cacgtacatt
    43201 cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct tagatgctca
    43261 attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag atgcagttgc
    43321 tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta caggacatgg
    43381 gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt
    43441 accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg gtggagtacg
    43501 tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa
    43561 agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac
    43621 agactttgtt atagaaaatc actcgattta atgatacact atagaagaac ttttaagctg
    43681 taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc tgatcggcgg
    43741 cagttttctt accacgtgat cactatgccg catcatagag aattcggatg tatctcgtgc
    43801 ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt aaacgcaaac
    43861 caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat cgggattgca
    43921 gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa tgtcactgaa
    43981 ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga agatggcttg
    44041 aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt agcaatggaa
    44101 atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt atctgaagtc
    44161 aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat cgaagaactt
    44221 tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga ttcgggaaat
    44281 ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag aagatgcgat
    44341 tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat cagagtttgt
    44401 tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg aattggataa
    44461 agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa aaatggattt
    44521 ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac cagcggctgt
    44581 tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt cacgtaaatt
    44641 cgtcaacggt gttttatcca atgtattgaa aga
//
LOCUS       DACELF010000011        42777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.011, whole genome shotgun sequence.
ACCESSION   DACELF010000011 DACELF010000000
VERSION     DACELF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.011"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            87..371
                     /locus_tag="IW687_000610"
     CDS             87..371
                     /locus_tag="IW687_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0949898.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(652..1071)
                     /locus_tag="IW687_000611"
     CDS             complement(652..1071)
                     /locus_tag="IW687_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0949899.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1073..1657)
                     /locus_tag="IW687_000612"
     CDS             complement(1073..1657)
                     /locus_tag="IW687_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0949900.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1704..3179)
                     /locus_tag="IW687_000613"
     CDS             complement(1704..3179)
                     /locus_tag="IW687_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0949901.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3360..3671
                     /locus_tag="IW687_000614"
     CDS             3360..3671
                     /locus_tag="IW687_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949902.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3786..6122)
                     /locus_tag="IW687_000615"
     CDS             complement(3786..6122)
                     /locus_tag="IW687_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0949903.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6264..6932
                     /locus_tag="IW687_000616"
     CDS             6264..6932
                     /locus_tag="IW687_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949904.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6935..7978
                     /locus_tag="IW687_000617"
     CDS             6935..7978
                     /locus_tag="IW687_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0949905.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8215..9096)
                     /locus_tag="IW687_000618"
     CDS             complement(8215..9096)
                     /locus_tag="IW687_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0949906.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9255..9983
                     /locus_tag="IW687_000619"
     CDS             9255..9983
                     /locus_tag="IW687_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0949907.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10139..10456
                     /locus_tag="IW687_000620"
     CDS             10139..10456
                     /locus_tag="IW687_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0949908.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10449..11057
                     /locus_tag="IW687_000621"
     CDS             10449..11057
                     /locus_tag="IW687_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0949909.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11112..11903
                     /locus_tag="IW687_000622"
     CDS             11112..11903
                     /locus_tag="IW687_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0949910.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12041..12529
                     /locus_tag="IW687_000623"
     CDS             12041..12529
                     /locus_tag="IW687_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0949911.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12580..13452
                     /locus_tag="IW687_000624"
     CDS             12580..13452
                     /locus_tag="IW687_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0949912.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13628..13990
                     /locus_tag="IW687_000625"
     CDS             13628..13990
                     /locus_tag="IW687_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0949913.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13999..14751
                     /locus_tag="IW687_000626"
     CDS             13999..14751
                     /locus_tag="IW687_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949914.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14765..16567
                     /locus_tag="IW687_000627"
     CDS             14765..16567
                     /locus_tag="IW687_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0949915.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16844..17885
                     /locus_tag="IW687_000628"
                     /pseudo
     CDS             16844..17885
                     /locus_tag="IW687_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(17937..20081)
                     /locus_tag="IW687_000629"
     CDS             complement(17937..20081)
                     /locus_tag="IW687_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0949916.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20438..21811)
                     /locus_tag="IW687_000630"
     CDS             complement(20438..21811)
                     /locus_tag="IW687_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0949917.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21811..22206)
                     /gene="rbsD"
                     /locus_tag="IW687_000631"
     CDS             complement(21811..22206)
                     /gene="rbsD"
                     /locus_tag="IW687_000631"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0949918.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22234..23151)
                     /gene="rbsK"
                     /locus_tag="IW687_000632"
     CDS             complement(22234..23151)
                     /gene="rbsK"
                     /locus_tag="IW687_000632"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0949919.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23259..24272)
                     /locus_tag="IW687_000633"
     CDS             complement(23259..24272)
                     /locus_tag="IW687_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0949920.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24353..24952)
                     /locus_tag="IW687_000634"
     CDS             complement(24353..24952)
                     /locus_tag="IW687_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0949921.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25138..25839
                     /locus_tag="IW687_000635"
     CDS             25138..25839
                     /locus_tag="IW687_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0949922.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25849..28560
                     /locus_tag="IW687_000636"
     CDS             25849..28560
                     /locus_tag="IW687_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0949923.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29011..30639
                     /gene="treC"
                     /locus_tag="IW687_000637"
     CDS             29011..30639
                     /gene="treC"
                     /locus_tag="IW687_000637"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0949924.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30699..31880)
                     /locus_tag="IW687_000638"
     CDS             complement(30699..31880)
                     /locus_tag="IW687_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0949925.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32132..33655
                     /locus_tag="IW687_000639"
     CDS             32132..33655
                     /locus_tag="IW687_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0949926.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33724..34452)
                     /locus_tag="IW687_000640"
     CDS             complement(33724..34452)
                     /locus_tag="IW687_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0949927.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34465..35746)
                     /locus_tag="IW687_000641"
                     /pseudo
     CDS             complement(34465..35746)
                     /locus_tag="IW687_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(35887..36489)
                     /locus_tag="IW687_000642"
     CDS             complement(35887..36489)
                     /locus_tag="IW687_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0949928.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36734..36940
                     /locus_tag="IW687_000643"
     CDS             36734..36940
                     /locus_tag="IW687_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949929.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37252..37971
                     /locus_tag="IW687_000644"
     CDS             37252..37971
                     /locus_tag="IW687_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0949930.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38052..38735)
                     /locus_tag="IW687_000645"
     CDS             complement(38052..38735)
                     /locus_tag="IW687_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0949931.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            38953..41139
                     /locus_tag="IW687_000646"
     CDS             38953..41139
                     /locus_tag="IW687_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0949932.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41485..41895
                     /locus_tag="IW687_000647"
     CDS             41485..41895
                     /locus_tag="IW687_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0949933.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            41909..42265
                     /locus_tag="IW687_000648"
     CDS             41909..42265
                     /locus_tag="IW687_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0949934.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        13953 a         7522 c         8540 g        12762 t
ORIGIN      
        1 aaaaaaggaa ctgcgacaag acttttgtca cagttccttc ttttttttgc tatgctatac
       61 tagatgtaaa aaataggaga aataaaatga gggaaattta ttcttttgaa tcaaaaatct
      121 atgcttccga agtcggcaag ggcggggcat atattatttt tccgtatgat ataagaaaag
      181 aattcggtgc tggaagagtt aaagtccaag caacttttga caatgtgcca tatgaaggca
      241 gtatcgtgaa tatgggcgtg aaaaatccag atggttcagt ctgctacatc cttggactac
      301 gaaaagatat tcgtaaaaac atcggaaaag aaatcggtga tattgtagca gttacagtga
      361 agcaaaaata aaagaggttg tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc
      421 aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac
      481 aaaatttttc ttactactcg aaacgaagcg gctttatcac aacctcttga taaacggcgt
      541 tcaatcagct gtccctcggt attagctcaa aaacggcaaa atatgagaag ctatttttcc
      601 atttttttct ctcatactca ggtcataacg ctgatttcac aacctcttct ttcataatgg
      661 tttttcacca tctttataat aaactggttc tttgatatgg aaatctattt tttccaacca
      721 atctaaaatc ttgtaaccta actcttctga catcggaaaa gaggcgttga tttgtgcatt
      781 ggagatatgg tcaacatgga taccggaagt gatcgtacaa cttcccggaa cggcagtttt
      841 gatgatcaca gctatttttt cggctaagac atcgtcttta tggaaccgac catcgtggct
      901 agggaaacgt acagtttgca tctctgtatc acgagaaaat gtagtgactg ttccgatatg
      961 tggtgtatct ccgcctgtta aagtgatcaa taagtcttta ccgattactt taacagcagc
     1021 ttggatcgtg aaacctagtt tggttacttc aactatcgtt tctttttcca tattaatcac
     1081 cctgccaacg ttttgaatgc tcatttacaa tttcaaattg atccaaaagt ttcattgtat
     1141 ggtgattaac aagatcttgg atcgtttgag gctgattata aaaagcagga attggtggta
     1201 tgatttgcac acctagtcgt gctaatttag tcaagttttc caagtgaatg gcatttaatg
     1261 gcgtttcacg aggaacaatg actaactttc gttgctcctt cattgttaca tcagcagcac
     1321 gtgcaatcag gttgtcgcct aaaccaacag agatacctgc gactgttttc atacttgccg
     1381 gtacgatgat cattccatcg gtcaaaaatg atccgctggc gacagcagca cctaaatctt
     1441 tgtttgaata atggtaatca aacaggcttt cgaattcagt tagtgataag tcggattcca
     1501 gctttaagtt ctgttttgcc catggactga tgattccgtg tgtttctatc atcggatatt
     1561 cttttagttt cttcactagg ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa
     1621 tcacgatttt tttcttccgt tgtgccaatt gttccatttt ccgcactcct ttttatgatg
     1681 tcaaaacagt tgcagatcat tcattatttc aaatattttt cccaatcttc tacttcttgg
     1741 aattttgcac gtacaaaatg ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt
     1801 tttgcactta tcccacggaa acgaatagag ccaggattat attctggtaa ttctgatggg
     1861 tccaatggat ggttcctcat tcctgggatc acgatgatat ctttatctcc ttggaaacga
     1921 gtgttgaaag tccaaactac atcgttcatg tcgaaaatat caacatcttc atcgactaaa
     1981 atgactgtct tcaattcttt gaaggctgat aaagcaagaa tagctgcttg acgctgaata
     2041 ccttcatccg cttcgttttc tttacggatt tgcatgatgg tcatcaattt accaccacct
     2101 gcaggaggat tgtagacatt tactactttt cctggaatag ctttatcgac caaggcaaga
     2161 atacttgctt ctgtaggaat accggccata gatacgtgtt cttcactggg accaatcgtt
     2221 gtttgcatga ttggattgtc ttttcggtga gtaactgctt tgactttcac tacttgtaca
     2281 gctggattag catccccagt atagccagga aattctggca ttgcttttcc tgagttcgtg
     2341 ttgatatctt cttgcattgt ttcatttggc atgatttctg cttcaatgat gaattcagca
     2401 cgagcaatac ccacttcatc gactgctata ccatcaacga tttgaatagg ttcgttacgc
     2461 aaagctcctg cacaccatag ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc
     2521 gtgccaatgg caattgctgg atctaaacca atattgatag tgataggcat tggtttattc
     2581 aaggcttcga attgtttttg gaagtgacca atgtggcgtc cgccaggcat gatgtacata
     2641 ccgatcgtat ctttatcttc tagaaccata cgatggatcg ttacatcgct cattgttttt
     2701 tctggatctg atcctaagac tacacccatt gtgatgtatg gaccagcatc ttcaggagta
     2761 tttgtaggtg caggcaaaat gttgcgaata tcgaagtctg catcggtagc tagatgaacg
     2821 acttcttgtg ctggagcgtc tgctttgtct actttcacag gtttgattgg attttctaca
     2881 gcgtctttca agaaacgacc aagtgttcga taatcatggt gcaaaatttc acctacacgt
     2941 ttacgactag ccattgttcc gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg
     3001 aaagtcatag ttggtccttc ttgagtcgga cgttttactg taccgccagc accgatataa
     3061 cggtaaacac cagataattc tgcttcagga tcgatttcaa tatctgtttc gtgatattga
     3121 cctggaagtg tttttagttc ttcgatgact tttcttaaat catacggttg atctgtcatg
     3181 agagattcct ccttagttgt ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca
     3241 attcaacttg ttcggtaaag tattctacaa tggaatggat gcgttttttt gataatagaa
     3301 taaactatga tatacttatc actaaaagaa agcgcatact tagtgttgga ggaaggaaag
     3361 tgaaaaaaat ggaggagtca tttcctaaag cagtaaaagt ggaaaatata gcgaatattt
     3421 tgaaagttac atttgaaaac ggagaagtaa aatacgtgaa atctcattgg acagaagaga
     3481 taaccgatgc cttgcaattt ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac
     3541 tttctcgtaa tatgtggatt gggactgaag taacgattga agcagatgga acagtattca
     3601 taaatggaaa agacaggtat acgccagaag agttgtggta caaaggaaag aaaagtatac
     3661 cagaattata aaaaataatt aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg
     3721 tttcttgctg ttttctacta tttcagcgct cttatgactc aaatctaaat gtggttgtca
     3781 caaccttatt ctgcacgcaa agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga
     3841 agatgaccgc caagcatcgt caatatagta gagacgatca ctagaataat cgcatggaca
     3901 ggatttagtt gagcaacatt ttttagatcc gtcatgttgt ataagatgct attgataggg
     3961 aacgtagcta gccatgcaat aaacacacct aatatcccag aagaaatacc taaaatacac
     4021 gtttctgcgt caaatacgcg agtgatgtct tttttacgtg cacctaaagc ttttagtaca
     4081 ccgatttctt ttgtccgttc aatcacagat gtataagtaa tgatactgat catgatcata
     4141 cttgttacta aagagatccc tgcaaaagca ataaggacat aagtgatagc atccatcaat
     4201 ccgccagtca gttcagtcat cgttccagct aaatcagtat aaatgatttt atcttcatct
     4261 gatttgcctt tattgtaatc atctagataa tccaagatct tttctttgtc ttcaaaattg
     4321 ttcggataaa tcatgatact tgaaggcaag ctgtctccac ctaaataact aagcaatgtt
     4381 tgtttggcat tttcgtcaac tttttcagta gttaagacat ttacaccact gtctctttga
     4441 gctttgacga tttctgattc tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta
     4501 taagcgattc ctggagataa gagattcatt gtagaagaag atttcacgcg taggatcccc
     4561 gagatcttca attcatcaga agcattctga tacatcgcat cataatctgt attagggata
     4621 aaattcccag ttggaagttt tgtatagtaa gtattgttgt ttactaattt gaatgttgta
     4681 ccaacgatat catcaaaatc aagtttttcg tcttctttca catcaaatcc aagattcttc
     4741 aatgcgttga tattagtatt attattgccg tctacgatca gcacgacatc tgttgctgag
     4801 gctggataac ttccggcaag taaagaataa ttgtctttta aaaagttttc tttgctagtg
     4861 tcaagctgtg tagggaaact tgacactccc acacctgtca ttgctgacat cgtactagat
     4921 aaagaaagag attccgtatc aggattttga ttcgagaagc tgacaggctg tactttgcca
     4981 tttacatcac gtaaaagatt gattcctgta gtacgggtaa atccgatatt attacttaag
     5041 ttaggatcga tgtctgttac gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt
     5101 gctttatctt cttcgctgac ttttgcagta atcgttttgc tgtctggata atccgctttg
     5161 cttgcaccca atccggcatc gtcacgagtc tggttagtcg ttaccttaga aatagtaatc
     5221 ggaaatttcg ccatcgtttc agcttgcgtg ttatcgattt gtttttggaa tccagaagaa
     5281 agggacagaa cgatcgcgat tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa
     5341 gtacgtcctt tttttgttcg gatattgttg aaggaaagct ttaacgctgt ccaaaaactc
     5401 atctttgttc tgcgtaagtt aaattgatca tcttttggtc gttcgatgtg aggatgggaa
     5461 tctgttaaga tcttaccatc agaaaattca atgattcgat ctgcgtattg atgggccagt
     5521 tcaggattat gtgtgaccat gatcactaat ttttctttcg ataactcttg gatcaattcc
     5581 atgatctgga tacttgtttc tgtatctaac gctccggttg gttcatcgca caaaaggata
     5641 tctgggtcat tggctagtgc tcgagcgatg gccactcgct gcatttgtcc accagataac
     5701 tgatttggct ttttatgcat atgatttgtc aagccgactc ggtgcaatgc atcttctgcc
     5761 cgttttcgtt tttcgtcttt agaaacacca cttaatgtca tcccaagttc aacgttttca
     5821 ataatgccta gatgaccaat caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt
     5881 cggtaggcat cccagtcact gtccttaaag tctttagtag actttccgtt gattaccata
     5941 tcaccggaat cataattatc tagtccacca atcacattca gcatagtcgt tttaccagaa
     6001 ccactaggac ctaaaatcgc tacgaattct ttttgtctga aagcgacaga cacaccatcc
     6061 aatgctttgg tggtggtttc gccgacttta taatattttt ttatatcttt taattgaagc
     6121 ataatcaccc ttctcccgta ttgaaatatt cggtatacta ataagtatat cagaacaatg
     6181 atcgtagtat acgaaataaa tgttaactga agatgaacta caagaaaaaa tctagaaatt
     6241 tttcaaaaaa cagagggaga tcaatgaatc gtattttagt agtagaagat gatccgcaat
     6301 tacagttgct ttaccgttct gtgttggaac gagcgggctt ttcagtcatc accgcattga
     6361 atggagagga tgcgctgaaa aaactagaag cgacacgtat agaattaatc attacagata
     6421 tcatgatgcc gaaaatggat ggctatgact tgctagactc attacgtagc accagatttg
     6481 acacacctgt tctaatgatt acggcaaagg ccgattttga ggataagaaa cgcgggttca
     6541 aattaggagc agatgattat atgacaaaac ccatcgatgt caatgagatg atcctacgtg
     6601 tggaagcatt gcttcgacgt tcaaagatca attacagcag tagcttgata atcggacaaa
     6661 caaaattaga gctagatgct tatacagtgt tacaagatca gcattcgttg attctgcctc
     6721 aaaaagaatt tctgcttctg tataaattac tgtcttatcc caacaagatc ttcactcgcc
     6781 aacagttgat ggatgatatt tggggattag atacgaatac agaagaacga acgatcgatg
     6841 tacatatcaa acgtttaagg acccgttttg aaaagacgga agattttcaa atcatcacag
     6901 tacggggact aggctataag gcggttattt aaagatgaaa aaaaagctat tttcacaatt
     6961 atggatctat tttgctagta ttgtcttcat ttcgatctta gtgacactgc tgtgttttct
     7021 cggttttatt ttcttattgt ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc
     7081 aacgttatac ccgttattcg tctttgcggg gttcagtatg gtcgtgggta caggaatatc
     7141 gatttttgtt gggcggcgta tcctgcatcc aatcagtgcg ctagggacaa atatgagtct
     7201 ggtagcaacg ggagattttt cgattcgaat ggatgaacag cagaaagtag cagaagtaca
     7261 gcaactgtat aaagatttca acgttatggt ccaagaactc aatagtatcg agacactgag
     7321 gaatgacttt gtgtccagtg tatcccatga attcaaaaca ccactagcaa caatccaagg
     7381 atatgttcaa ttgttacaag ccccaaatct ctctgacgaa gaacgccaaa tatttttata
     7441 tcggattatc gaaagtatca cgcagctttc acaattaaca gagaacatct taaaattgaa
     7501 caaactggaa aaccagcgta tccaactgga aaagaaagag tatcgattag atgaacaaat
     7561 cagagaagta atcgtttttt ttcagcctaa atgggagaaa gaacagctag aattggatat
     7621 cgaacttgct gcagtaaatt acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa
     7681 tatcatggat aacgccataa agtacaatca aatcaatgga cagattcata tcaaactatt
     7741 tgagacagcc acagagattg tactggaagt gaccgattca ggtgtaggaa tgaatgaaga
     7801 gaccagagat cgaatgtttg aaaaatttta tcaaggagat acaagtcgtc aaatttctgg
     7861 gaatgggcta ggactttcac tggttaaaaa aattctagaa ctgcatgatg gacgtattga
     7921 ttatagcagt atcgaaggag taggaacgac agcaatgata cggttaagta aacaatagga
     7981 aaaagaataa aacccctaaa aattctattg aaggttttta ggggttttta gtcgtttttt
     8041 tgtgattagg gaaatgttct tacggttctt ttggcataaa aatcgctgct ttcttattga
     8101 actacgagaa atgttagagg tgtcattgaa acaaggacat gatgcaagaa agagagcgaa
     8161 agtcatgcgt cgctctcttt ttttgggtgg atagatggac agaaaatgta gggcttagtt
     8221 ttcttcttga tttaaatact cccaaaatgt atctgaagcc aaagaatgct caccgacttt
     8281 acgagtaaat gcaagataac tctctgttgt tggagaaata gggagaatca cagtattttc
     8341 ttttaataga ttcttgactg ttttttccat aacaatagca atccccatgt catttgcaat
     8401 catattgatg atcaagtcga ttcttgctcc tttataagag atttttggtt caaatccagc
     8461 ttcgcgacac agatctagta caggttgata caagttcgta gtggggccaa ggagcaaaaa
     8521 gttctccttt ttcagttcgc ttagatccaa tttcttttta ctagctaaag gatgattttt
     8581 aggtagaact gctacaaaat cgtctgtttc agtaggcaac cactcaatcg aacttggtgg
     8641 ggcctggaaa cttctagcaa agtggatcgt gtcactatgt tcattggcgt cattgtaagt
     8701 taattcgaca ctttccactt ctttcaactg gactgtaaat tcaggatggc gtttcaaaaa
     8761 ctcggtgatt ttggaaaaac ctttataatt gatcattgtc ggtatcgtta agatatttaa
     8821 tgaaagattg ttgtctttcg tataggtttc aagcacttct tgcatagcat ggtattgttc
     8881 tacgacattt tttacatatg ggagcaataa gcttccagct tcggtcaatt cgatttttct
     8941 attggcgcgt tcgaataatt tgacgtctaa ttctgtttct aacgaaagaa tctgtttaga
     9001 aatatttcct tgggtggtga attgtcgatc agcagttttg gtgaaattca ttgtttcggc
     9061 taaatcaaga aaaacttcga atttatggat atccatcata ttcctcctac ttattccaat
     9121 aaagaatact ttaacacaaa aaatgaattg gtgtaatctt ctctgtctgt tacaatcttt
     9181 cttgcagcga aagcaataat tacagaaatt tttctgattt ttttcgcttc gtagaaaaag
     9241 agaggagttt tttgatgcca actggagttt tgatcaatgt aggttccgtt ttgtttggcg
     9301 gactaattgg cggattagta ggaaataaat taagtgaaca ttttaaagca cagctgacga
     9361 tggtttttgg tgtgtgttcg atggggatgg gaatctattc cattgcgccg atgaaaaata
     9421 tgccggctgt tatttttgct ttagtgatag gaacagcgat tggtttgatt gttcatttag
     9481 gtaatgggat aaataagggg gctgcgctga tgcaagtacc tatttctaaa atcttcccta
     9541 gtgaaaagct tggtatgacc cacgatgaat ttatcagcac attagtgacg gtcattgttc
     9601 tattttgtgc aagtggtacg gggatctatg gaagtttaga ttctgggatg acaggagaca
     9661 gtacgatttt gatttctaaa tctgtccttg attttttcac agcagctatt tttgcgtgta
     9721 atttagggta tgtcgtttct gtgatagcaa ttccccaatt cattattttt tatattttgt
     9781 tcttattggc aaaatttatt tatccattga ctacaccaga catgatctta gattttaaag
     9841 cttgcggcgg attcttgatg gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc
     9901 cagtagctga catgatacct gcaatgatcg ttatcatgcc actgagctgg atgtggacaa
     9961 attggattat gccactgtta taataaaaat caaagagaag ccattttttg ctgactaaaa
    10021 aggatcaaat cttttagtca gtttttttgt atttgacaaa ctgattttga aactatatta
    10081 tatgttgtat aatatagatt gaaataaaat attacacttg agaatagaag gtgacgtaat
    10141 gcacatccag attccaactg ttcttcttga tggtactgtg ctagcagcat taagcaagga
    10201 agatatgtat gggtatgcct taacaaaaat cgtacaagaa agtctctctg tcagtgaatc
    10261 tacactatat ccggttttga gaagattgaa aaaagaagga tatcttgaga cctatgatga
    10321 accttttgaa ggcagaaacc gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt
    10381 agaagaaatt attgatgaat ggattgtttt tcgagattct ttagaccggt tattggagga
    10441 gagcacaaat gaatgaatat ttgaatgaag ttgtggatca tcttcaagaa attcctgaag
    10501 acgatcgttt tgatttgatc cagtattatg aggaatattt tttagattct ggaaaaacat
    10561 tagatcagat cattgatgaa tatggtacac caaagcagtt tgccttaaaa ttaaaaatca
    10621 gttacttttc agaagaagaa caagcagaaa acgagttagt gaaaaatcca aaaagacaaa
    10681 ttcgcctgat ttggttaatc attatcggtt tatttgcttc tcctgtcctc attccactag
    10741 cactggggtt tatattggtt attgggggac tattggttgc tttactagcg ctcattttta
    10801 gtatctatgt tttatgtatc agtatcttag gtgttggcct attcatgatc atctcaggaa
    10861 tcagtgtgtt tggacaatcg attctgagtg gattattctt tatcggtgtc ggtctgttcg
    10921 ccacaggaag cgcagtattt atttcgccac tgttgctgaa aggaacaaaa gggctttttt
    10981 atgtcatgat gaactttgtt aaatggatgg gacgacgttt tattacaaaa agagatctgc
    11041 attcagcggg cgtttgaagg aggacacata aattgagatt gaaacatttt ttgattaccg
    11101 gtattggtct gatgcttact ggtggcctcc tagcaggtgc aacttataca ttaggcgctc
    11161 aaaagagtct gacttgggat aatggtcctc ggttgatcga tatggttact gagacaaaaa
    11221 aaatcaagga tgcaaacaaa atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac
    11281 gcggattgga ttttgagata aaaacgactt atgataagac agaaaagcca gaaataaagg
    11341 agaaaaatgg cgtgttgact gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt
    11401 tagaagacac aagtgcacaa atcactattg ttgtccctgc agatcatgaa ttatctgaat
    11461 tgacaatcga agggaataat agtgcaattg atatcaacga agtatcaatc aaaaaagtga
    11521 atactaagac acatgatggc tgggtatcgt tcaatgaatt tacaggagga acaataactg
    11581 cgaaagatga attaggctca cttcgattag atgaggccaa tgtggataac ttgatcgtca
    11641 caggtgaaaa cagcaacttc catctagaag acagtttgat cagcaaagaa ggaaaagtca
    11701 tcctaaacgg tggcaattta tctttgcatg agatggaaaa cagtggctat actatccaaa
    11761 cgtctggtaa atcttatatt gaaaaagact accagccaat aacaaatact ttgttagaac
    11821 aaggacagaa aaagctaaag atcactgcta aagacacatc gatcaatatt acgacggatc
    11881 aagaggacaa tgaaacagat tgatcctgta ctcaactgga tattttaaga aaaaacagca
    11941 aagatccttc caatctttgc tgtttttcat cgcttaacgc acataactat agtaaaataa
    12001 gaaagaaaat atctaaaaag aaaacagggt gaaagagtat atgaaggtag aagaaggtca
    12061 aagagaaaag ttaaaaacag aaatggaaag attacatact tatatcactc aactaagcca
    12121 aaccttctat gatccagaca aagaaaaagt gatggttaat tacccgaaca attcagaagg
    12181 tcgccaatta gagcaagtgt accatgaagt tttcaaacat ttattgacgg tgaagaaaga
    12241 gctggattat tattctttac cgattatcga tacaggaata ttgaagtatg atgggaagaa
    12301 ggaacgattt atctttaaat ctgttcggga agatttgcct ctttcagcag ggatggatct
    12361 agaggtcttg gtagaagact attttacaga agaaaagcac tgggttcgaa caaaattgga
    12421 ttatttaccg caggcagccg gagggacaca ggcaagcggt tggtacatca ctgaagacaa
    12481 agagttagaa ctagaaggtg taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat
    12541 tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa aagagcattt
    12601 tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt tggattgttt
    12661 ttcttctatt taggtatgtg gcaagcgaaa gtttgggcac tttttttaag tatttttctt
    12721 tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa
    12781 gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt tttccttacg
    12841 atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt
    12901 ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt agtctttaaa
    12961 gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt tgtagaaatc
    13021 caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac attcaatgag
    13081 aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca aaaagagctg
    13141 caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc
    13201 tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc cattttgtct
    13261 gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt agagcaaatc
    13321 gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat caataattta
    13381 cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg cgacatgaaa
    13441 gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg gattattgtc
    13501 ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt
    13561 gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta agagagaagg
    13621 ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg tacttgcagg
    13681 ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac aaattacgac
    13741 agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca actatagata
    13801 tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca agggacaatt
    13861 gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt
    13921 cacttcgtat gaagaagtcg aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat
    13981 taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac aaaagtatac
    14041 ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa
    14101 ggcgaattta ttgggatcat gggcgcttct ggttctggta agtcgacact tttgaatatt
    14161 ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa agatgtgacc
    14221 acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt tattttccaa
    14281 gatttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc tctttcttta
    14341 caaggcgtca gaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc
    14401 atctctcata ttttggaaag ctatccttct gagatttccg gtggacaaaa acaacgggtc
    14461 gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga gcctacaggt
    14521 gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt gaatacaaaa
    14581 gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt
    14641 attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga tcaaagccgt
    14701 gattccttct atcgagaaat actatctatt cttggaaact tggaacagta gggggagatt
    14761 gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag actacttggt
    14821 gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat caatggcaag
    14881 caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca ttttccgatt
    14941 cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa attccttttt
    15001 gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctgggagcaa aaggcagaaa
    15061 aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta ctttagtggg
    15121 ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg atcagttaga
    15181 tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca ctgttttgtt
    15241 ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc
    15301 gattttagca ttgattcgtg cggatcagac accaacaaga atcaagcagc ataaattctt
    15361 gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact atatgatgaa
    15421 acacgtgggg acctatcaag tctttggtat aggggtagcc ttagttacga tcgtattagg
    15481 aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa agaaatcaga
    15541 acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact ttcgtattcg
    15601 tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac tgggcgcttt
    15661 gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca attctggata
    15721 tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag aactttcacc
    15781 aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact ataatgcaag
    15841 tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac cgccgaagat
    15901 cacgaaagaa aaagttcctg ctaaaaaaat gaatgaactg actgtacaag aggaacttcg
    15961 aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt
    16021 tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag acttccaagc
    16081 aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc catctttgca
    16141 aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg gtctattctc
    16201 tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc ttgctagctg
    16261 tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg aaatgcttga
    16321 aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt
    16381 attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac aaatgtttgg
    16441 tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatat tcttcgtatt
    16501 gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat tgaaaaagag
    16561 attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagctcctca
    16621 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
    16681 gaaaaagagg ttgtgacaat atttttgtca caaccttttt ttcacggtct tttattagaa
    16741 tatagtaaat tttttctcgt tactcacgga ataagctgtt aagaaaacgg tttccatgta
    16801 tgctggaatc agtgcaaatg aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca
    16861 aaattttggc gaaaactatc acttgattac gatcacgaat caaaatggga catctttgtc
    16921 tgtcagtgat cttggggcac gggttgttca ttttcaaaca ataatcgata atgaaaaacg
    16981 agagttgatc ttaggttttg atgaagcaaa agaatatcta gaaaaagacc cttatatcgg
    17041 tgcttcaatc ggacgtatgg ctggacggat tacgaatggc acattcaaat tagatgatga
    17101 aacgttcgaa gtaaaaacag acccggacac tggtcattgg tttacatggt ggtgcaccta
    17161 gctttgaagc caagcagtgg cattatacag tcatcaatgg tgaaaacgaa gcatcggtca
    17221 ttttctatca gactagccca gatatggaaa acggatttcc tggaacttta gatgtagaag
    17281 tacggtatac gctgacgaac gatgatacat ggcgagtgac ggtccaaggg atcagtgacc
    17341 gcaaaacagt attcaatcct acaaatcatg tctatttcaa tttaaccggt gatccagcac
    17401 agtcaatcga cgaacatata ctttggttga atagccgatt atttgctacg ctcaataaag
    17461 atacaacgcc tacaggagaa gtagtttcag taagtggcac agcatttgac ttccaacgtc
    17521 ctaaaaaatt aggtgaggtc tttgccagtg attttcgcca atcaatgata gtgggcggca
    17581 tcgatcatcc atttttgttg aaaaaacaat ctggtttact aaatgctgcg gaattgacaa
    17641 gcccagatga aaaagtatcg atccaagtaa aaacagatgc ttcaggaatc gtgattttta
    17701 cagcaaattt tggagaagat ggaccggaaa tgcgcgggaa taaattagta aatcatggcg
    17761 gtatcacatt tgagactcaa gaactgccag gagcggagcg acttgaaagt tttggagatg
    17821 tcactctgga acctaaccaa gtacgcgagt ttgtaacgga atacaaaata aatataaaga
    17881 aatagaataa attaaaaagg gagtgtgaca gtgtcacatc ccctttttaa tttattctat
    17941 ttctttaaag gcagatcaag ccagaatgat ttgtctaaac ttaacaaggt tagtacagga
    18001 acagttgcag ctttaactgt acgagtagtt gtttctggta tctgtcttgc cgtaaaattg
    18061 ttccacaaat cccagattga agcgacacgg aagatggaag aagcgttgtt gacgcgtttt
    18121 atcgttgaaa attctaatcc tgtgattggc acgccatctg ttgtccattt gattggcaca
    18181 tcgaatcgct tagacatatt gaggatttca gcgtttgctt cttcagttgt tgctgcttct
    18241 tttttcggtt catcatattt tgtcaggtcg tacgcttcga tgatcgcgtt caatttttgt
    18301 gaatattggc gatctgtagc ataacgtcct tccaaatatt tggttgcttc tttgtagtct
    18361 tttgtttccg acttccaagt tggtttgtag aaatcaggat tgccagtcag tccatttttg
    18421 atcaagtcgg catagtcttc caaactttcc ttgtaagaag gatatttacg gaattgtgcg
    18481 cggatttggt agctttgtcc ggaactatct tgttcaagtg tattgaaggt cacgctgctt
    18541 cctttatatg atcctttgat accaaaaagg ttatgatttg gttcgcttga taataagctg
    18601 tttccagatc cactttcaag gatagcttga gccatcatga ctgatgcgta gattccttct
    18661 ttttgcccga tttcctgtgc atcatctgca atctttttca agaactgatc agttgtttgg
    18721 ttttgactga aatggaacga gccatctaat ccatctaaaa tagatgagcc gcctgtcagc
    18781 gaacctgaag cagcaggtaa acctgctaag tttactcgga cagcaaaatc aggcgtgaaa
    18841 tctgaaatag aagttactcg aactttatct cctggttgag gcgcatggat aaattgtccg
    18901 ttcccaatat agatacctac atggtgagta acaccggaag aaggtcccca gaagacgagg
    18961 tcgccaggtt gggcctgact tactggaatc tgcgtacctg catattgctg ttcgcctgtc
    19021 cagccaccga tataatgacc ggttgctttc atatatacgt aataagtcaa accagaacag
    19081 tcaaaagtat ttggaccttt tgctccccat acatatggtt ttccaagatg cttgaatgct
    19141 tctcgaacga ttgcttgttg gacattcgtt gtatttgtag gcaatacagc atcaattggt
    19201 gattttacaa caaaatcaga agaagcagaa gatggattct ctataccgct ttgatttgct
    19261 gtatttggac gagatgaagc attgttcgtt ccgccattcg atgaaccatt gtttggatgg
    19321 tttggttctc tctgagtatt tccattgtta ttcgatccag atgggtttgt ttgattcgaa
    19381 gaattcccgc tgttcgaatt attgttatta ttgtttgaat tatttgaacc attattattg
    19441 ttgttattac ccacatctgg tttgtttggc gtttccggat ctggtgtact agggacttct
    19501 tcactaggat cagttggttt ctctgttgaa gtatccggtc cagctttaaa ttctgcagta
    19561 atagataaat tgttcgtacc tactgtaaag gtctttgcat cataatttac ttctttggca
    19621 ccattaacag cgactgaagc aatttgttca ccttcagctg gcgtaatagt gtaagttacc
    19681 ttcgtttccg ctaataaacc ttctaccgtt ccactttccg taatatccaa aggttgctgt
    19741 tcaggggaat ccgctgtcga agcagtcagt tggatcttac caatcaaaga aggagcaatt
    19801 gtaagggaat atgtctcggc tggcttttca ttttctgatg gatcagccgg atcaatttca
    19861 gttggctcgg tcgatcctgt tgttgagtta gttgttccag ttgggtcttc tgtaaaagaa
    19921 tccgttgttt cactagtagc tgagctggat gtagtcgtat ctgttgtgac tgtttgatca
    19981 gctatttgat tatctacggc aaatacggta ttggtcgaca aaacagacgg agcaatcagc
    20041 caagtagcag ctagcaaacg tatcgttttt ttatttttca taataaacta gtccttttcc
    20101 tttcattgat tttctgtctt attcagcatt cattagacat tttttggaac agataatcgt
    20161 gtcgaaaagc tttttctttt cataaatctg tctgtgtatt ctaaataaaa caatttgtaa
    20221 ataagcaacg agcattcatt cgtttttctt tgacgttcat tttattgcat agaccacctt
    20281 tgtttgggga taaacaagtg gggataaaaa tggttatgca tatgtaaatt ataccaaagt
    20341 ttggaaagta tcacaaaaat ttataaaaat aatgatccta aaaagaaaaa gactgaaata
    20401 tgtgataaac atctcatact tcagtctttt tcattaacta ttcataagac attaggtctt
    20461 catcaaaccc gctaacgaga gtagtgatca ttgctacgac tagactaaca gccagtcctg
    20521 ctaagtacca gaaaaaagta ccggaaatga atgcaggcaa tgtggtcaga ctaccaaata
    20581 caaaagcgat cgctttcact tgggcaaatc ccattacagc tccaccagca gctgcaccaa
    20641 tgcaagcagc aacaaatggt tttttcaatg ggatcgcaat tccgtaaaca gttggttcgg
    20701 tcactcccgc aagtaaaatc gaagcaaaag cagaaccgga gatcgaacgg gtttttgaac
    20761 gtttacttcg cagaaataca cctaaagctg caccagccat tccaaaattg gctgctcctg
    20821 tcaacggaac gattcggtcg aacccataag cagagatatt actgatcatg acagggttta
    20881 cagcccattg gataccaaat attaccaaaa tattccaacc accagcgatg attgcgccac
    20941 tgatcggtcc gttaatatcc atcatccaag taattgcggc agctaaacct tcgccaaggt
    21001 aaacacccgc aggcccgaat accattaacg tcaaaggaac cgtgataact aatgaaagta
    21061 aaggtacaaa tactaattgc atattttcgc gaatatattt tttgaggaat ttttcaacaa
    21121 aagaataaaa gaaaatacct aaaatcgccg gcatgacagt agacgtatac tgcatcagta
    21181 ctacaggaat ccccagaaaa tcagtagctg ctttgccact gtctgaaagt aaagaggtca
    21241 gattcggttc aatcagtgct gcagcaatga cagccgcaat atatccattc actttaaacg
    21301 ttttggcagc agaaaaagca agaaagattg gcagaaaata aaagacggca ttcgacgcag
    21361 cagacaaaat atgataagtt ccgcttgttt cactaagcca gccaatcgta gtcgctaaaa
    21421 caaccagacc ttttaatata ccagaaccgg caaacagcgg aatgatcggc gtaaagattt
    21481 ccgatatcaa accaaatagt ttttcgatcg gatttagctt ttctttcgga gctgaatgat
    21541 cgatttgacg cgtcaattcc ttgtaagcag gagccaatcc ctgatccatg attatttctg
    21601 catatctatc gagtaaaatg aaatacttaa tggaagggat ttgctgtttt aatttttctg
    21661 cattggctac tggctgtttt ttcaatttga tacgaagccg agtcgcacat ggaaggacag
    21721 aatcaatatt gtcttttcct cccaactgtt caatcagcat atgaatctgc tcggacagta
    21781 ccatctgttc aggattcatt gtcgtttcca ttagaagacc actccactca ctaatataat
    21841 attggcgtaa ggtgtcattt cgcctgtacg aataaatgca tgacagttat tcaactcttt
    21901 tttcatttct tcatgtggga taaagctaat cggtgtatct ggtaacaata atttgatttt
    21961 ttccaatatt tccgggttca ttgtctttat ttcttccgcc aattcaatcg attgtacctc
    22021 aagttcttct aaaacgtttt ctaaaacaga taaaaaactt ggaagaccgt ttgagacagc
    22081 caaatctatt ttttcagtac caaaggggac aggcatgcct gcatccccga tacttaattt
    22141 atcaaagtgc cccatttgtg cgattactcg tgaaatatct gaattgatta cttttccttt
    22201 tttcatgttt gtttcttcct ctcattgtta attttagact gctgattgtt ctaaagcttc
    22261 gcggtaaggg atagaaggtt gcgcaccata agactgcaca gcaacagaag aggctagatt
    22321 tccataaagg atcgcttctt tcaagttact aaggtctggc tgaagtgtgc tgcttaatgc
    22381 gccaataaat gtatcacctg cggctgttgt gtcaacggca tccactttaa aagcagggac
    22441 gataccgtgt tctttttctg tatgatagaa agcgccggca ctgcctagtg taatgattac
    22501 tgttccaata cctaattcat ggagtttttc ggctgctgcg accaaactgt tatgatctgt
    22561 cactctgatt cctgtaatga tttctgtttc tgtttcattt ggtataatga tatctgtctt
    22621 ttcaagtagt tcagtaggaa tcgtttcacg agctggtgca ggattcagga tcgttgtttt
    22681 tcctgcgtct tttgcaatag aaaaagctag gatcgtcgca tctaagctac tttcaaactg
    22741 agcaataata aagtcactgt ttgtaatcag ttgcttgttt tttaggactt cttgttcatg
    22801 gaaagcatta tttgcccctg catggatcaa gatactattt tctcctgctg cgtctaccat
    22861 aatcattgct tgtccggttg tggcgccttg gatttcagct atcccagatg tatcgatgtt
    22921 ttcttttgtc agtagatcta ataactgttg tccttcgctg tcagccccaa cgcctccaat
    22981 aaaagatgtc cgtgcgcctg aacgtttggc tgccactgct tggttggcac ctttacctcc
    23041 gccagcgtga aaaatctcat gcgcatgcat cgtttcgcct ggcttgggca tgttggttaa
    23101 acgaagggta gtgtccatat taatacttcc aattactgta accgtgttca ttttacaaac
    23161 ctccaactat ctcatttatt ttgatgggaa aattattttt taaaacgttt tagtaaaacg
    23221 ttttaatagt tcgataaaaa tatagcacac cttgaaagct atttcaaggg tgcagttgaa
    23281 aatctttttt ctaatcgtac aggtaatctt ttttcttccc attctttttc tgggaattgg
    23341 atccgatcta acaataactt ggcagatgtt tgtcccaatt cgaagattgg ttgtgcgatc
    23401 gttgtcagtt tcggcttgat atactcgcac atatctatat tatcatagcc aataatgctg
    23461 tagtcttcag ggatgctttt tccagcttcc tctaatccgc gatataatcc aaaagctaat
    23521 tcatcattta aagcaaacac accagttgca gcagaatcaa gaagttctgc tgtgatctgg
    23581 tagccacctt gttttgagaa ctgtgtaggt aagaggatca gttggtcgtg agggatttgg
    23641 taaacatcaa gagcagattt aaacccctca atccgagcgt ggacattttc cggtggattt
    23701 tcaggataga caagggcgat cgtttgatgt cctagactaa gcaagtgcat gcccgctagg
    23761 taaccaccgc gaaaatcgtc tgttcgtacg gcatcactaa atccttcaga ttttttctga
    23821 tccaaaacaa tgaagggccg gccttgtttt ttcaaatttt cgttgattgc atctgtagag
    23881 acagcagaag tcgcgataat gaatccatcc acacctcgac gtgtgagctc ggctagatat
    23941 tcgatttctt tttgatgatc agaatcagca ttacataata tcgtgacgaa attctgctta
    24001 tacaagatat cctcaattcc tcgcatcagt gtactgaaga atggattcgt gatatcagga
    24061 acgagtacac caattgtttt cgtttccttg gtaatcattt gtctagcaaa atagtcaggc
    24121 tgatagccta attcttcttt ggcagctaaa actttctgaa cagttttcgg actgaatttc
    24181 tccgtatttc catttaatat caaggagaca gttgtgattg aaacgccgga aagtttagcg
    24241 acttctttga ttgtgatctt ttttttcacc atgttcatac tcctatctaa atcctgtggt
    24301 tattttccca tgaaatcaaa aagaagtaaa tgaaaatcac atgtgccagt acttatttgc
    24361 tttgttggat agcttcgatt atttgtttcg ttgagcgtac tcgtgcagtt ttcggaaata
    24421 tcttttttat gaacaattga tgggattctt tgtcacgatc actgcaagca tcttcagcaa
    24481 gcacgatatg gtacccgtgt tgaaaggcat ctaatgcagt agcatataca ccgtttgttg
    24541 tcgctacacc gcttaaaatg atagtttcga ttccacgccg gcgaagttga agatcaagag
    24601 aagtaccaaa aaaagcactt ggattgtatt ttgtgatagt caacatatta tctgtatctt
    24661 tcaaagaatc cttcaaaagt aaatcagtga attctggagg tacagaagca gtcattccac
    24721 ccatatcagt ttgttgactc aaataaggga aagtggagat gtctacgttc actaaaactt
    24781 tcaaggcagc ggtacgtttc aatgttctta ctaacaaatc attgtttgtc agaatagatg
    24841 ctgcagtgtg tggagagagt gtaccgttat ttacgatctc tttttgcatg tcgatcgcga
    24901 caaaagcagt ttttgataaa tcaaaaatca tattttcctt taaatcattc atgatgttcc
    24961 ttctttcaaa taacaatgtt ttttatgtaa gtaaatcata aatcgtttat ttgtcaacta
    25021 aattaaagga ttcctttctt tagtctagag aaattcagtg gtaaaataaa taagtgtgag
    25081 aaaacgcatt cgatgcatag atatagcgag aaatatcgtg aaaatggagg aatcagagtg
    25141 aagtatgtcg aagtaaaaaa tgaatacaaa cgatacaaaa tgggagaaac gaccattaca
    25201 gcaaatgacg atatttcttt ttcaattgaa aaaggcgaat tagtgattat acttggtcca
    25261 agtggagcag gaaagtcaac gattcttaat attttaggtg ggatggatac acctgatgaa
    25321 ggtcagatca ttattgatga cacagatatt gcgcaatttt cagataaaca gcttactgct
    25381 taccgacgaa cagatgtagg gttcgttttc caattctaca atctcgttcc taatctaaca
    25441 gcaaaagaaa atgtggaatt ggcaacggaa gtttctcctg atgcgcttga tcctgtagag
    25501 gtattgaggc aagtgggcct agctcatcga ttgaataact ttccgtccca attatcagga
    25561 ggcgaacagc agagggtttc aattgctcgt gcactagcta aaaatcccaa acttctttta
    25621 tgtgatgaac cgacaggcgc gctggatttt gaaacgggca agcaagtatt gaaattattg
    25681 caaaacgcta gcagacaaca cggcaatacc gttttgatca ttacacataa ttctgcttta
    25741 gcgcccatcg ctgatagagt cattcacatt aatgatgcta aagtaaggtc tgttgaatta
    25801 aatgatcatc cttcatccat tgatgagatt gtatggtagg gggattttat gaaaacaaga
    25861 acatatctta aagcaggttt tcgcgaaatt cggcaatcta aaggacgatt cattgcaatc
    25921 atccttattg tcatgctagg aacactttta tttgtaggtg tcaaaactgc tggacctgtc
    25981 atgcaaaaaa cgatggatca ctatgttggt acagcaggac tttctgattt gcaaatcgtt
    26041 tctactggtg gcttgacaca aaaagatatt gctcaagcag aaaagatccc agatgcaaaa
    26101 gtagaaacag gaaaacagat ctattattcc aatactggca aaaacgaagt gattcaaata
    26161 ttttcttata acaaaaataa caagcagaac aagctacaat taactgatgg aaaactcccg
    26221 gaaaaaccaa accagcttgt attggatgaa aaagcggaag atgaagggta taaaatcggt
    26281 gatatttatc agattgattc agatgaattg aaagaaaaag agtataccat cacgggcttt
    26341 gtccgttctc cattgttcat caacaatttg gagagaggat acgcgaacgt cggaaatggt
    26401 agcgtcgatt attttgtcta tttacctgaa tccgttttta aaacagatat ttattctgtc
    26461 ttatatcttg attttacaaa cgtaaagaat ttgaatacgt acagcaaagc ttacaaagat
    26521 aaaatggagc aaaatcaaga aaaggcagaa aagtatttac aagatcgacc cgaagaacgc
    26581 ttagaagagc tgaaaaaatc ggccaacgaa gaattagaac cggcacagca aaaaatagcg
    26641 gatggcaaag cacaaacaac gcaagcaaga acacggttag aagatgctaa aaaacagtta
    26701 gcgcaacagt cagcaactat ccagcagctt cctgaagcgc aacgaacaat cgctgttcaa
    26761 acattatctc aacaagaagc tcaacttgct gaacaagaaa ggcaattagt tgagaaagaa
    26821 cgtgaactgg cctctgcaga ggaagaattg aatcgaaatg cagaaaaaat tgaaaattta
    26881 actaaaccat catacctata caatgaacgt tcagaaaatg tcggttttca agaatttggt
    26941 gatctgtctg atcgtatcgc tgcgattgcc aatgtttttc ctgttttctt tttctttatt
    27001 gccgcattga tcacctttac gacaatgaca cgtatggtag aagaaaaccg cagggaaatc
    27061 gggacattaa aggcgttagg ttatacgaaa gttgaaatcg caaaaaaata tgcaatctat
    27121 gcaagtctag cctcagggat aggtattgtt ttaggaacga tattaggaac gaatcttttg
    27181 ccaaggatca tttttgagct gtctaacgag cggtatgata ttgggagtgc gcttattttt
    27241 tatgactggc cgccgatttt acaagcagct gccgcattct tcattgccgc atttggcgct
    27301 gcaatgatcg ttttattcaa agatttaaga gaacggcctg ctgcattgct tcaaccgaaa
    27361 gcaccgaagc cagggaaacg catttttctt gaatacatca ctccgctgtg gtcacgcctt
    27421 agctttaatc aaaaaatcag ttaccgaaat ctcttccgat ataagtcaag aatgcttatg
    27481 gcgattatcg gaattgctgg atgtgccggg ctgatggttg caggcgtggg attgaaagat
    27541 tctctaagtt ctgtttcagc caaacaattt ggaacgatca ttgactatca agcaatcgtt
    27601 acattggatt catccacgga agatagtcgt caaaaagcag cggatatgct gcagaagcaa
    27661 gaaaaagtaa atgagatcct agctattcat actcaaaccg tagaattgag aaaaaaagga
    27721 catgcttcgc aaagtctgac agtgatcgta ccaagtaaaa cagctgattt ggatcgtttt
    27781 atccatttaa ctgatcttga agagaaccca cgctccttgc ctaaaacagg aatcgctatt
    27841 acgcaaaaag tagcagaact atttgactta tctacaggag atcatatttc gctttatgat
    27901 gaagatcaaa aagaatggaa agtcgaagta aaagaaatcg tgaaaaacta tctaggtagc
    27961 tttatttata tgtcccctga atattatgct tcagttactg atcaatcgat gatggataat
    28021 gcatttttgc tgcgtacaga taagatgaca acaaaagaag aagaaatgct ttccgagaaa
    28081 ctgcttgcca cagacgctgt gacaaatacg tctttcatgt ccaaacaaat cgaaacgcaa
    28141 gaagaatcaa tggcgaactt agactcggtt gtcttaatct ttgtcgtatt atcaggactg
    28201 ctggctttta tcgtcttgta taatctaact aatatcaata tttccgagcg tgtacgcgaa
    28261 ttatctacaa taaaagtcct tggatttttt gataaagaag tcacgatgta cattgttcga
    28321 gaaaatatca tctttactct tttaggaatc atcggaggat ttggtgtagg atatatattg
    28381 actgacttca tcttgcaaca agcgtctatg gagaatgtca tctttccatt agtgatcaca
    28441 tggtgggctt atgtcttatc agcaggatta actatcgtat ttacggttat tgtgatgatt
    28501 gttacccatt tcaaattaaa gcatatcgat atgattgatg cgttgaaatc gaatgaataa
    28561 aaacagaggt tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa cggcaaaata
    28621 gtttttcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccatg
    28681 aatcagaggt tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca aataaaatta
    28741 gctcctcatg ttttacattt gagaagactg attgccgtag ataaaagctt ttgaacacca
    28801 tttatagaag aaacaagaat attttgctat tttaaacaag aggatagaca aatacatgtc
    28861 tgatctcttg ttttttattg ggcaacagac ctcttagaat gtataaagat aaattttaca
    28921 aacgctttcg gttcagttat aataataaat gataacttgt atatataagt tgtggtaggt
    28981 aataaatcag ttgaatggag gtaaatatcc atgacattta aagacaaagt catttaccaa
    29041 atctatccca aatcttacaa agacagtaat ggtgatggaa ttggagattt aaaaggaatc
    29101 aaagaaaaac tcccgtatct gaatgatcta ggcatcgaca tgatttggct aaaccctatt
    29161 tatccaagcc ctcaaaaaga caacggttat gatatctctg attacacagc catcgatcct
    29221 atctttggta cgatggagga attcgaacag ctaatagccg aagcaaagaa ataccggata
    29281 gaaatcatgc tcgatatggt gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa
    29341 gcactagcag gggaccagca ttatcaggac ttcttcattt taagagaaga gccaacggat
    29401 tgggtatcaa aatttggtgg aagtgcttgg tcgccatttg gagatacggg gaaatattat
    29461 ttgcacttat atgatcggac acaagctgac ttgaactggc ggaaccaagc agtaagagag
    29521 gaactattca aagttgttcg tttttggatg gaaaaagggg taaaaggatt ccgtttcgat
    29581 gttatcaatg ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa
    29641 cctgaatata cggacaaacc aatcacccat acgtatctaa aagaattgaa ccaagcaacg
    29701 tttgggacgg attccactat catcacagtc ggtgaaatga gctctacgac gatcgaaaat
    29761 tgtatcttgt atacagaacc tagtagagaa gaattatcaa tggtctttaa tttccatcat
    29821 ttgaaagtcg attatgaaaa tgggaataaa tggtctactc ctccatttga ttttgcagaa
    29881 ttaaagcgac tatttcatac atggggagaa aagatgagtg agggtaacgg ctggaatgct
    29941 ctatttttta ataatcatga tcagccacgt gcgctcaatc gatttgtagc tatcgatcag
    30001 tatcgaaaac aaggagcaga gatgctagca accatgatcc atttgaaccg tgggacgccc
    30061 tatatttata tgggagaaga aatcggcatg atcgacccag actattcatc gatttccgac
    30121 tatattgacg tagaaagttt gaatgcttat aagttactcc ttgaacaagg attcacagaa
    30181 aaagaagcgt ttcgtcggat caaagcaaaa tctcgcgata actctcgaac acccatgcaa
    30241 tggtcagaca cagaacaagc aggatttact acagggaaac cttggttaaa agttggaggc
    30301 aagctagaag agattaatgt agaaaaagaa ctatcttctg aagattctat tttgtcctat
    30361 tacaagaaat tgattcggct gagaaaaaca tatccaatcg ttgcacaagg agattatcat
    30421 gcttatgaag caaatcatcc ggaagtttat ggctttcttc gccaatttga aggacagcag
    30481 ctgcttgtat taacaaattt ctatgataaa gatacaagga tcgccatccc agaaggattc
    30541 acagaagcca atattcttat cgataattat tccgatactg ttcttcatca aagtctggag
    30601 ctaaaaccat accaggcaat tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt
    30661 tcctacgagc tattctcgta agatcgacgt tttgtgtttt atttaaaata atcttcttca
    30721 gaaaatgaag agttttcata ctctccttta tcgccataaa atttattgta gcgttgtttg
    30781 aatttacgga aaataaaagt aacgagtaat ttgactaacg tatagatagg aataccaaaa
    30841 ataacgccca caattcccat tagatcaccc atgacaagta aaacgattaa gatcgtaatc
    30901 ggatggatct gtaaacgatc acccattact cgaggaatca ccaagtcacc gtggagaagt
    30961 tgtacggcaa accagacaat gacaaatttg acagccataa aagtagagtc ttggaatgca
    31021 atgatcaaac ctggaaagaa ggcgataaac ggcccaatat atggaatgat acataacaac
    31081 ccagcagaaa tagctaaaac actagcaaat tctaatccga tcagtaagaa acaaacccag
    31141 tagactgctc caaggataaa tgaagcaatg atttgcccct ttagaaatga acccaattgt
    31201 tgattagcaa tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg gacaatcgct
    31261 aaaactgact gataaaattt ttgaggatct ctcaaaagga aaaaagcgac aattggacca
    31321 gtaaataaag taataaaaat agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg
    31381 ccactttccc aataatttcc aataaatcca gctatatcag acgtcacgtc gtcaatagaa
    31441 gcgaaaaatt gattaaatga atcggcaaaa ggtgttttct ccaaaaattg tcggagactc
    31501 tcttgaaaat cttgaaacaa atctggcaaa ctactgatca atgtctttgc ctgttcttca
    31561 agaaaaggaa taagccatat gcttccaagc cccaataaaa ataaaataat cacataaacg
    31621 ataatgactg acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa
    31681 agataataca agatcagtgc aaacaataat gggggcaaga tagtagcgat aatgattccg
    31741 acaggcttga atataaacga gatctgatgg aaaaaataaa tgatcagcgc aagtaagata
    31801 actattccta aaacatagta agaggttttt cctcctaaaa agcggatcaa acgattcgtt
    31861 gtatctgttt cttttttcac tctttttcct cctttatcta taacttcatt atagacaaaa
    31921 gcgggtataa aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat cattcatata
    31981 aactttctca tcaattaaaa taagaaaacg cttgacagtt cttttaacca tatgataaat
    32041 taaatcctgt tgctaaaacg taactattac ggtttgcgaa aagcgaatcg gaacattcaa
    32101 atgaaagaag atgagaagag gagtgtcata aatgaaacga ttgataggta tattggccat
    32161 gctggtttta gcaggtatgt taactgcttg cggggcaagt gggaaagccg aggacagtaa
    32221 agaaaaattg agcgtgatga cgacctttta tcccatgtat gattttacaa aagcaatcgt
    32281 cggggatgaa ggagaagtag agttattgat tccagcaggg actgattccc acgattatga
    32341 accttctgct aaagacatgg caaagatcca agatacagat atctttgttt ataatgatga
    32401 aaacatggaa acatgggtcc ccgcaatcca aaaaacatta caagaaggca atgtccatac
    32461 gataaaagcg acagaaggta tgcttttact tccgggaagt gaagaaggac atgatcatga
    32521 ccatgaacac ggggaagaag gccatacaca tgagctagac ccacatgtat ggctagctcc
    32581 aagtttagca atcaagcagg tagcaaacat acgtgatcag ctgattgaag cttacccaga
    32641 aaaacaggaa gtatggacaa aaaatgcagc agcctatacc gagaaacttc aagcactcca
    32701 tcagctttat caagaaacct ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc
    32761 tgcattcaac tatctagcgt tagaatatgg attgaatcaa gtatcgattg ctggtctttc
    32821 ttctagtgaa gaaccgtctg cggcgagaat tgctgaactg aaacactttg ttaaagaaca
    32881 tggaatcaac tatatttatt ttgaagaaaa cgccaaagat agtattgcca gaacgttagc
    32941 aaacgaagca ggtgtatctt tagaagtatt gaatcctttg gaaggattga cgaatgaaca
    33001 aatagaaaat ggagaaaact atctttctat catggaagca aacctcgaag cgttgaaaaa
    33061 aacgacagag acagaaaacc cgctagaaga tactttgacc cccaaaaaag aaaagagcgt
    33121 ctacaacggt tattttgaag acgaggatgt acaagatcgc tctctttcag attgggcagg
    33181 aacttggcaa acagtagata cgtatgtaga agatgggact tttgatcctg tttttgaata
    33241 taaagaaaaa ttgaatcagg ataagacagc acaagaatat aaagaatatt atacgaaggg
    33301 ctatcaaaca gatattgctt caatcaagat cagtgagaaa gagatggcat ttacgtttaa
    33361 gaatggaaca acgaaacgtt caaattatcg ttatgtaggg aaagaaatct tgacctacga
    33421 ggcaggaaac cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt
    33481 tagatatatt caattcagtg atcacacgat tgcaccggct gactccgaac attttcattt
    33541 atatgcagga aatgaaagtc aagaagtatt gctgaaagaa atggagaatt ggccaacatt
    33601 ttatccagat acaatggatg gacgtgaaat tgcacaggaa atgcttttgc actgataggc
    33661 aaaaaagaac cgaagggttt ggatcggctg gtcatttagt cggtccaaac tattcggttc
    33721 tttttatgaa atcaatttct ttaatccacg gatagaggct gggatggtct cgccgtttgc
    33781 caacgtgact tctctggttt ttttattaat cgaccgcaca ttttgtgtat tgataacata
    33841 ggatttatga caacggataa aattccgatg cattttctcg atttctttga tcttctcata
    33901 gaattgtaat tgggcatgag tcaaatggac ttccagttta tgtggcgctc ctagcgaagt
    33961 agaaaaatat tggatgtctt tgatcggtaa aaatttgatc tcatcattaa actctatttt
    34021 gaagatatct ttttggattt gggacatttg aaaatcttcg aaaattttgc tcaggatatg
    34081 ctcgacattt tcttttaaat cataaagatc ttctttgacg atataatcag tcggttcgat
    34141 gttgcttttt aagattttca tcgacatttc cgtatgggaa gtcacataga ttatttttcc
    34201 taaaggatct aattcacgga tcaatttacc gatcgctaca ccatccattt cactgtcttc
    34261 tagttcgata tctaaaaagt aaataccaaa agaagaagtt ctgatcttgg ctgcattgag
    34321 aagttcttcc ggatcagagg tagaaatatg gacataactg tctaagtttt caatcatgat
    34381 tctatttttg atcaccgttg ataaaacttc taattgtttt ggatcgtctt cacaaatata
    34441 aatgggaatc atatcattac tcctttattt tatttgtata taaatcgttt gagaaaaacg
    34501 ctggttttca attgatgttt caaggaaaag ataaggattg gtctttgtca attcatccaa
    34561 gataaacaag ccaaggcctt cgttttttgg atttgttttt gttgagaacc ctttttgttt
    34621 caactggttt aacggtaatg tatttgttgt tgtattgatc acacgaatca tcacggaatg
    34681 attcttcttg agtatggtca attcgatttt tttcttcttt gcagcactag ctccttcaat
    34741 cgcattgtcc aagataatac caagcatacg tacaaggttt accgtatgtt tcgtatccag
    34801 ttcgattggc tcaggaaaat gaagctggac gatcaaccct ttttcctgtg ccatcatgag
    34861 ttttaatgac agtaaactac ggatctcagg aactttcata ttatccaaca tacttagatt
    34921 ggccggaaaa aggttcatca tctggcgtgt aggtaatatc gtttgatcat aataagaccg
    34981 taatccttct aagtcgcctt ctgaaatata gctgtgaaga gaaaaaagca gattttggta
    35041 atcatgtttg aattctctca agtcttgata gtttttttct agatctttga tataatcctg
    35101 ttgcatcctg acttgttgct ctaataactg atcagctaat ttcttattag agattttcca
    35161 ataataaacc aagatgatca ggccaacaca cagatataga atgaaaaaac tcgtattcaa
    35221 agctaagaca tcaggtgttt caccagaaaa acgggtataa aaaataatga tatagtatgt
    35281 aaataatatc aatgtcccga tcgtaccgat catacggcgg tccacagtgt gaaaccaagt
    35341 ctctaacgat ttttttaaac aaaaagctaa taaagcagat aaaaaagcgg ataagacaat
    35401 gtgaaaatac tgcaatgaat ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc
    35461 gccgatgctt gttaagtgat ctcctaaaag cgacgtcaat aacccaaaaa gaaagcgcgt
    35521 aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag
    35581 caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa
    35641 tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac
    35701 aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc
    35761 attgattttt accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt
    35821 ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt
    35881 aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt
    35941 aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta
    36001 agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt
    36061 taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata
    36121 gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt
    36181 caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag
    36241 acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga
    36301 aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac
    36361 cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac
    36421 catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt
    36481 atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc
    36541 ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt
    36601 cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg
    36661 acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga
    36721 tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa
    36781 atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt
    36841 gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg
    36901 gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga
    36961 gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa
    37021 cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt
    37081 ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga
    37141 gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt
    37201 atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata
    37261 attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt
    37321 gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg
    37381 ctattttatt ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa
    37441 cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc
    37501 tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa
    37561 gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca
    37621 caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga
    37681 atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac
    37741 tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa
    37801 atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc
    37861 gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc
    37921 cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac
    37981 taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg
    38041 attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc
    38101 tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac
    38161 agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga
    38221 tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc
    38281 gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt
    38341 ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg
    38401 ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga
    38461 acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt
    38521 agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat
    38581 tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa
    38641 gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt
    38701 cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat
    38761 ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta
    38821 aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag
    38881 acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag
    38941 gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg
    39001 gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc
    39061 tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa
    39121 agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat
    39181 caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga
    39241 gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa
    39301 caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta
    39361 aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat
    39421 agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa
    39481 ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag
    39541 ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct
    39601 cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa
    39661 gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg actgtatgga
    39721 ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta
    39781 ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa
    39841 tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg
    39901 aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt
    39961 atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct
    40021 accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca
    40081 aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa
    40141 aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg
    40201 ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata
    40261 ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt
    40321 ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa
    40381 catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag
    40441 gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc
    40501 gatcaatact tttctcctaa atatcaaaat atatccgttg cagattatat tttgtcgata
    40561 agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa
    40621 acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata
    40681 gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct
    40741 actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa
    40801 acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac
    40861 aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg
    40921 ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca
    40981 gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat
    41041 cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt
    41101 atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga
    41161 agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga
    41221 aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg aaaagggagg
    41281 tgtgacaaga attttgtcac acctccctta tttattttga aaatgagaat actattatca
    41341 aaatagagaa tatatagaaa aattctcatt attcaaacgt aaataatcag ttattttgat
    41401 tttttttata ttttattagc taataattgg tattttaaaa gaaattactg tagaattgga
    41461 ttataattaa aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc
    41521 tgtagaaaag caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt
    41581 tttgccgaac cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga
    41641 gttcaagata ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac
    41701 gaattaactt taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga
    41761 cctattttga ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca
    41821 tttgtccctc gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac
    41881 atcttaaatt catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg
    41941 tgtaacaagt ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt
    42001 tgacttagaa gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga
    42061 agtgcaagca gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga
    42121 cctttcctgt gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct
    42181 cagacgtcct ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat
    42241 ggaacagctt atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc
    42301 atacagttgt aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt
    42361 tatttttcta ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg
    42421 atatagagtt aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt
    42481 tttaagtagc aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg
    42541 ttagaaatga catgataaga taactgagtt gttaaaaaag aaccacttgt gaagcttgat
    42601 tctatcgaaa aaaacttcaa aaaagttctt gacaaataac aaatgataag ttatgataaa
    42661 tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa
    42721 ttacttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggg
//
LOCUS       DACELF010000012        41210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.012, whole genome shotgun sequence.
ACCESSION   DACELF010000012 DACELF010000000
VERSION     DACELF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.012"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW687_000649"
     CDS             complement(26..328)
                     /locus_tag="IW687_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0949935.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW687_000650"
     CDS             complement(353..2446)
                     /locus_tag="IW687_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0949936.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW687_000651"
     CDS             complement(2935..3699)
                     /locus_tag="IW687_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0949937.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW687_000652"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW687_000652"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0949938.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW687_000653"
     CDS             complement(4704..5780)
                     /locus_tag="IW687_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0949939.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW687_000654"
     CDS             complement(5804..6397)
                     /locus_tag="IW687_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0949940.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW687_000655"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW687_000655"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0949941.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW687_000656"
     CDS             complement(8067..8366)
                     /locus_tag="IW687_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0949942.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW687_000657"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW687_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0949943.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW687_000658"
     CDS             complement(10121..11032)
                     /locus_tag="IW687_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0949944.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW687_000659"
     CDS             11152..12009
                     /locus_tag="IW687_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0949945.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW687_000660"
     CDS             complement(12196..12558)
                     /locus_tag="IW687_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949946.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW687_000661"
     CDS             12904..14343
                     /locus_tag="IW687_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0949947.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW687_000662"
     CDS             complement(14350..16902)
                     /locus_tag="IW687_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0949948.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IW687_000663"
     CDS             complement(17016..18347)
                     /locus_tag="IW687_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0949949.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IW687_000664"
     CDS             complement(18313..19791)
                     /locus_tag="IW687_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0949950.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW687_000665"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW687_000665"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0949951.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IW687_000666"
     CDS             22401..23828
                     /locus_tag="IW687_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0949952.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IW687_000667"
     CDS             complement(23920..24285)
                     /locus_tag="IW687_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0949953.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IW687_000668"
     CDS             complement(24282..24647)
                     /locus_tag="IW687_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0949954.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IW687_000669"
     CDS             complement(24839..25087)
                     /locus_tag="IW687_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949955.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IW687_000670"
     CDS             25086..25214
                     /locus_tag="IW687_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0949956.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IW687_000671"
     CDS             25271..26965
                     /locus_tag="IW687_000671"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0949957.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IW687_000672"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IW687_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IW687_000673"
     CDS             27170..27361
                     /locus_tag="IW687_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949958.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IW687_000674"
     CDS             27739..28872
                     /locus_tag="IW687_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0949959.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IW687_000675"
     CDS             28903..29223
                     /locus_tag="IW687_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949960.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IW687_000676"
     CDS             complement(29252..29716)
                     /locus_tag="IW687_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0949961.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="IW687_000677"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="IW687_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="IW687_000678"
     CDS             complement(31133..31450)
                     /locus_tag="IW687_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0949962.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="IW687_000679"
     CDS             complement(31476..31796)
                     /locus_tag="IW687_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0949963.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="IW687_000680"
     CDS             complement(32260..33675)
                     /locus_tag="IW687_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0949964.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="IW687_000681"
     CDS             complement(33692..35008)
                     /locus_tag="IW687_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0949965.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="IW687_000682"
     CDS             complement(35158..35856)
                     /locus_tag="IW687_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0949966.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37134)
                     /locus_tag="IW687_000683"
     CDS             complement(36145..37134)
                     /locus_tag="IW687_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0949967.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW687_000684"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW687_000684"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0949968.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="IW687_000685"
     CDS             complement(38222..39856)
                     /locus_tag="IW687_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949969.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="IW687_000686"
     CDS             complement(39843..41177)
                     /locus_tag="IW687_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949970.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12504 a         8475 c         6959 g        13272 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaact
//
LOCUS       DACELF010000013        40688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.013, whole genome shotgun sequence.
ACCESSION   DACELF010000013 DACELF010000000
VERSION     DACELF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.013"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..166)
                     /locus_tag="IW687_000687"
     CDS             complement(<1..166)
                     /locus_tag="IW687_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0949971.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYE"
     gene            292..1161
                     /locus_tag="IW687_000688"
     CDS             292..1161
                     /locus_tag="IW687_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0949972.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            1187..1702
                     /locus_tag="IW687_000689"
     CDS             1187..1702
                     /locus_tag="IW687_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949973.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(1749..2312)
                     /locus_tag="IW687_000690"
     CDS             complement(1749..2312)
                     /locus_tag="IW687_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0949974.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2367..2546)
                     /locus_tag="IW687_000691"
     CDS             complement(2367..2546)
                     /locus_tag="IW687_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949975.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(2623..3132)
                     /locus_tag="IW687_000692"
     CDS             complement(2623..3132)
                     /locus_tag="IW687_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949976.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(3228..3608)
                     /locus_tag="IW687_000693"
     CDS             complement(3228..3608)
                     /locus_tag="IW687_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0949977.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            3938..4687
                     /locus_tag="IW687_000694"
     CDS             3938..4687
                     /locus_tag="IW687_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0949978.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(4871..6148)
                     /locus_tag="IW687_000695"
     CDS             complement(4871..6148)
                     /locus_tag="IW687_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0949979.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(6227..6871)
                     /gene="pcp"
                     /locus_tag="IW687_000696"
     CDS             complement(6227..6871)
                     /gene="pcp"
                     /locus_tag="IW687_000696"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0949980.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(7070..8014)
                     /locus_tag="IW687_000697"
     CDS             complement(7070..8014)
                     /locus_tag="IW687_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0949981.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(8016..8702)
                     /locus_tag="IW687_000698"
     CDS             complement(8016..8702)
                     /locus_tag="IW687_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0949982.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            9176..9496
                     /locus_tag="IW687_000699"
     CDS             9176..9496
                     /locus_tag="IW687_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0949983.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            9650..10555
                     /locus_tag="IW687_000700"
     CDS             9650..10555
                     /locus_tag="IW687_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0949984.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            10552..11325
                     /locus_tag="IW687_000701"
     CDS             10552..11325
                     /locus_tag="IW687_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0949985.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(11506..12930)
                     /locus_tag="IW687_000702"
     CDS             complement(11506..12930)
                     /locus_tag="IW687_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0949986.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            13141..13587
                     /gene="nrdI"
                     /locus_tag="IW687_000703"
     CDS             13141..13587
                     /gene="nrdI"
                     /locus_tag="IW687_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0949987.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(13652..15634)
                     /locus_tag="IW687_000704"
     CDS             complement(13652..15634)
                     /locus_tag="IW687_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0949988.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(15648..16544)
                     /gene="murQ"
                     /locus_tag="IW687_000705"
     CDS             complement(15648..16544)
                     /gene="murQ"
                     /locus_tag="IW687_000705"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0949989.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(16547..17608)
                     /locus_tag="IW687_000706"
     CDS             complement(16547..17608)
                     /locus_tag="IW687_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0949990.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(17819..18346)
                     /locus_tag="IW687_000707"
     CDS             complement(17819..18346)
                     /locus_tag="IW687_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0949991.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(18413..19915)
                     /locus_tag="IW687_000708"
     CDS             complement(18413..19915)
                     /locus_tag="IW687_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0949992.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(20173..21294)
                     /locus_tag="IW687_000709"
     CDS             complement(20173..21294)
                     /locus_tag="IW687_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0949993.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            21420..22361
                     /locus_tag="IW687_000710"
     CDS             21420..22361
                     /locus_tag="IW687_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0949994.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(22511..23407)
                     /locus_tag="IW687_000711"
     CDS             complement(22511..23407)
                     /locus_tag="IW687_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0949995.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            23676..25016
                     /locus_tag="IW687_000712"
     CDS             23676..25016
                     /locus_tag="IW687_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0949996.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            25234..25677
                     /locus_tag="IW687_000713"
     CDS             25234..25677
                     /locus_tag="IW687_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0949997.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(26181..27599)
                     /gene="nhaC"
                     /locus_tag="IW687_000714"
     CDS             complement(26181..27599)
                     /gene="nhaC"
                     /locus_tag="IW687_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0949998.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(28002..29225)
                     /locus_tag="IW687_000715"
     CDS             complement(28002..29225)
                     /locus_tag="IW687_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0949999.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(29256..30095)
                     /locus_tag="IW687_000716"
     CDS             complement(29256..30095)
                     /locus_tag="IW687_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0950000.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(30112..30792)
                     /locus_tag="IW687_000717"
     CDS             complement(30112..30792)
                     /locus_tag="IW687_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950001.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(30794..31867)
                     /locus_tag="IW687_000718"
     CDS             complement(30794..31867)
                     /locus_tag="IW687_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0950002.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(32514..33185)
                     /locus_tag="IW687_000719"
     CDS             complement(32514..33185)
                     /locus_tag="IW687_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0950003.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(33272..35950)
                     /gene="mgtA"
                     /locus_tag="IW687_000720"
     CDS             complement(33272..35950)
                     /gene="mgtA"
                     /locus_tag="IW687_000720"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0950004.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            36609..37046
                     /gene="ndk"
                     /locus_tag="IW687_000721"
     CDS             36609..37046
                     /gene="ndk"
                     /locus_tag="IW687_000721"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0950005.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(37068..37934)
                     /locus_tag="IW687_000722"
     CDS             complement(37068..37934)
                     /locus_tag="IW687_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0950006.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(38094..40187)
                     /locus_tag="IW687_000723"
     CDS             complement(38094..40187)
                     /locus_tag="IW687_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0950007.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        11817 a         8456 c         7202 g        13213 t
ORIGIN      
        1 cttcataagg agtcaattcc aatgcttcat agatatgata catatctgga tggatctttt
       61 gggacgcata ttcttttttt aaaaattctc ttagatgcag ataataatct tttgaaaatt
      121 ctgaggacaa aacttcttgc caactgttat gaatgatttc tttcaaaaac tccacctcct
      181 aataaaaaga ataactcaca ttttcttcaa tcacaaggga caatcttcac cctcagcact
      241 tgattaatat ggtaaactag gaacaagaag cagaaaggaa cgataatcat tatgatcaaa
      301 ttaattgcat cagatatgga cggcacatta ttagatgccc atatgagtgt ttctcctgaa
      361 aatgccgagg cgatacgttt tgcaaatgat gccggtgttg agttcatggt cgctactggt
      421 cgaaactatc aagaagctcg cgcagcttta gatgaggtag gaatcgattg tgcgatgatc
      481 actttgaacg gggcacaggt atttgataaa gaaggaaatt cattgtttac cgtgtcgatt
      541 ccaaataatc aagcaatctc tgttttagat attctagatg ctaacggtat ttactatgaa
      601 gtagcaacca acgatggtct ttattctgaa aatcaagcga aacgaattga aagttttgct
      661 tccatggtgg ctacccattt acctcacctt acgtataaaa tggcgattgc catggcctca
      721 gctaagctgg agttattgca catcacatac gtggacagta ttcgtgctat tttggaagaa
      781 aagaatctgg aagttttaaa aattatttgt tttcatacag aagggccagc tgtattaggt
      841 ccagttggaa agcagatttc tcttttaggc gatctagccg ttacttcctc tggtcaaaac
      901 aatatcgaag tgaaccataa aaacgctcaa aaaggaattg ctgttgccca tgtcgctcac
      961 gagcgtggga tttctttaga agaagtcatg acgattggcg ataacttcaa tgatgtcagc
     1021 atgctgcaag tagcaggagt cagtttcgca atgggaaatg cagaaatcga agtaaaagat
     1081 tatgccaaat atacaacgga cacaaacctt gaatctggcg taggaaaagc catattgcgc
     1141 gcgattgcag aagacttgta gttttatcta gaaaagaggg atatagatgt cagaattttt
     1201 cattcaggaa aagcagcttg gacgtgcaac tcggacaatt gtcaaaaacg aaaaaggaca
     1261 ttccctgttt ttgatggttg gtcgctgggg aacaaagggc gatgcattgt ctctttatgc
     1321 catgaatggc gatctcgttg ctcacatcaa gcagataagc ctgacattcg gtacgcgttt
     1381 tgagctgtat aaagagttcg aaaaggtggg aaccatgcag aaaatattca attggcccgg
     1441 tgacttttat tatattcgta gacttcactg gacagtccaa ggtgatatct ataatcatca
     1501 atatcaaatc catcatttca atcagcaaat catgaaaatg gataaagcaa cacttttttc
     1561 aggagattac tatgtattgg atgtctcaaa tgatgaagat gctccagttt gtatttgtat
     1621 tgctgcggtt ttagattact ggctctaccg taaagacaaa agtcgcgata agcctgtttc
     1681 ttaccgactt aacttaagtt aaaaagatgt acctgaaatg cggaaatacg catttcaggt
     1741 acatcttttt agatattctt ttgcatatga tcataaagat gaccactgat catccgctga
     1801 ccgacaactt tgaagtcatg gcgttcgtat aattttttag cctttggatt tttttcatcc
     1861 acacttagac cgatgacgga gcgattagct ttcttcgcca atttaggtaa tgcttctaat
     1921 aatcgcgaac cgatgccttg tcctctaaaa tcttctgata cactgataga gtccaaatac
     1981 cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg tagacgcatc tagctgatat
     2041 ttctctaaga taggtaccag tggctgatcg atgattgatt cttcttcatc tgtataaccg
     2101 taagcaactc ctgcaacttt accgtctatt tcttctacga tacctcgtga ataactgtaa
     2161 cgataatttg gatcagtgat tgcttcttct aaaatctcta aagtcttttg ttcccctgtc
     2221 tgcttcaaaa atggcagttc catatctttt agaatcacta agatcaatgg ggcgatttct
     2281 ggagcatctt cttttctcgc aaaacgaatc atagtttcct ctccttttct ccattctaac
     2341 atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat cttcaatctt ttcagtaatg
     2401 aaataaccat gagtaacgat tcttgccgct tcagttcggc aataagggca gacgactggc
     2461 atttctttat cattatgttc gactacatta ttgacttctt cgtatgcaaa tttttttccg
     2521 caattagggc atttcatttt ttccatgtta tctcaacttc ctttcatctt tactctctta
     2581 gcttactctt atattgtaaa aaagagaagc aatatccttc ttctatgcag atatccatac
     2641 ctgacggtcc tgttctccat ctattaatcg gactttataa taaggacgtt catctttgac
     2701 tagaagtgtc cactctttca cttttgcttt tttcccgtag tagctgactg cttccttcat
     2761 cgctgcttgc ggttgtttga actcttccgt tttttctata tcaaaaagat gttctgcggc
     2821 cttttccgtg tcatttcgta cggttgtttt cccagtggat ggattgactg ctatgacttg
     2881 tccttcacaa aaaaactggt attcatatgt tgcattcgta ggattctcca aatctttttc
     2941 aactgcaaaa tgaatagcag atatgttatg ttcttttgtt tcgtttttta cgattgctgc
     3001 tgcttttttc aatgagatcc gatattcctg gtcactaata tcatccatgt gaaaagcggc
     3061 attgagcagt cccgtcatac cggtaatccc tattacgatg agggcagcaa caccaaacat
     3121 catttttttc attcttcatt ctcctgtttc ttttttttaa agaatatcaa gaaatgaacg
     3181 aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt gttatcttta ttcgtgcagt
     3241 tctaatggca atccgtctgg atcaaagaaa aacgtcattt tttttcctgt aaaggtatct
     3301 tctctaatcg gttctgattc gatccctaag gactgcaatt ctgcaattgt ttcttcaata
     3361 tcgtctactt tgaaagcaag atgacgcaag ccacaagctt ctggatagtt tggtcgttgt
     3421 ggcgcattag caattccaaa aatctccaat tcagcacttc ctagtttcag atctaattta
     3481 tagtcatctc gttctttgcg gtaattttct ctgattactt caaatcctaa tttattaaca
     3541 taaaaatcac gtgatttctg ataatctgaa acaatgatgg ctatatgatg gatcgttgta
     3601 aggttcatat gagcactcct attcttcgtt taagctcgga tctatattaa tctccagttc
     3661 cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc ctttttataa gaaaaaaggc
     3721 aaaaaaaaca gaaagtaggt cctccaagac actcttcctg ttcccaatat aataaatgaa
     3781 agtaagaaat tatattttca agaaaacaaa cagaaaatta tcataacaca aattatttat
     3841 aattgaaaga ctagtttttt ttcaagaaaa gcatacaata gattgtgtta gtaaatacag
     3901 aacacactac atttagtgtc ttaaggagtc ctcatacatg aagtcatatt ttcagttttt
     3961 agctcatcag ctaaaaggct ggccgcaaca aaattattat cttttctttt tcagtcttgg
     4021 ctgccaggtc atgactcttg tcaatagtcc catcacttgg ctgtccgtta ttacatttat
     4081 cggtaccacg cttggtgtat tgtgtatttt atccatcaat gcggcaaaat cagtcaacgg
     4141 tattttaggt attttgtctg cgatttgttt tattatcgtt gggttttcag caaagaacta
     4201 tttaagtatc ggtgaacaga ttgcttatat gatcacattg gatatccccg tattgttaag
     4261 taaagaatgg aatcataata tggcttcaaa aatccgaaaa ttcactggaa aaagctggtt
     4321 tatcgcaatc atcagtactt tccttgtcta tcttgtttca ggattcctga ttggtcgtta
     4381 tacagatgat cctcggccgt gggtagatgc cattgctttt tctattagct tgactgctgg
     4441 tatcatcagt ttcttgcgtt acaacaacca atattactgg tggttagctt cagggcttgc
     4501 tcaaatggtc ctttggtata tttcatttac gcatggatct gcaagtttgg cgatgttcgt
     4561 gaacagctct gtttacttgc tgaatgatgt ccttgcattt accgtttcac cttggtataa
     4621 ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa tactacaacg cacaacaaca
     4681 cgcataaaaa aactgagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     4741 acgtataaac ggcggaatca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     4801 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     4861 cctctatttt ttatagtaat tccttcagat actttcttgc ctcatcattg aaacgcagtg
     4921 attcttctaa cttctgataa tattccaacg cttgtttctg agcttcagta aaacgataat
     4981 gttttccttt ccatttgtcg attgtctctt gcacatttcc agattggata tctttcacta
     5041 ctagttgcca tttttctttt gagactgtac ggtctttctt atcagcagat tgtatagctt
     5101 ctcgttcttt acgcaatact tttaaaaaac catttctgac tatccgagga atgctgtcga
     5161 agaacccttc tgtaaaacca tagcgacaat attgcttcca aataaataga tagatttttg
     5221 gttggatgaa tcgttggttt ttcgcaaaat attccatctg agcagtgact tctggtgttc
     5281 gttgacgttt tgtctttctc aacacggaaa taaatgtttc aaaataagca ttaggatgta
     5341 ctggcacttc ttcatatacg atggattgcc aatctttacg taaaaatgcg cgatagttga
     5401 tcaagaattc ttctacatcc tttactaatt cttctgcaaa gtcgtcaaat agttcttttt
     5461 ttatctgtcg cttttttgac tgcccattga tgtgttcact tattttcttg atttcatttt
     5521 ccaatgcttg ataatccgct atgtaaatcg gcatttctac ttggcgaaat tctgcgagcc
     5581 gctgagctaa ttttatatcc ggtaaatatg gctgtttcat ccgacgattt tggtacaaga
     5641 caaaacagta tttttgccat agtccttcag gaaaatcgat attcttctgc ttaagccata
     5701 ataattcgtc taaggccaga tcaacttttg tcattccctg atggatcgaa gctgccactg
     5761 tcggagagac atgcaggtga tcggcaaagt ggttgatgcc tagttttagg atctttccgg
     5821 tttttggcga tttaacgatg tattgcactt ttaattctct accacactca caatacaatt
     5881 tttgctggct tcgttttgtt cgaaaaaacg gattgatctt gaagtcagaa aacacccaat
     5941 ctgttgcagc taaataatgt tcttccataa aacgagagct gattagatat cggcggtgct
     6001 tatcgactag ttcccgctgt tttttcgtta attttgcata gatctcccaa cgttgactgc
     6061 ttgtgtattg tttagaaatt ttgatgggat agcttttgct gatttctttt ggaaaccatt
     6121 cttcaaagac ggattgccat tcttccacgg ctgtttcttt cctttctaat tacaaaaagc
     6181 tggctactaa cataacgtta gcaaccagcc agtgtttctt gctaaattag tgagttgttc
     6241 cggcatttcc ggaaatatct tctttatttt ctactgctgc ttcaactgca taagtcaaag
     6301 aagaagcaat gtcttctaaa gacatagaag gcgtacccac tggacgatcg atcacttgat
     6361 cgggtgagaa tgacacgtgg atgaacccac ctcgaattgc cgggaattgt gtttcgatca
     6421 aatgaagcaa acgatacatg atatcattgc agacaaatgt tcctgctgta taagagatgt
     6481 acgctggtag accatgatct tgtacatttt ttgtcattgc tttgattggc aatgaggtaa
     6541 agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa tggctgattt ccttcattat
     6601 cagggattct tgcttctgct agattgattg cgacacgttc gaacgagact gctgaccgcc
     6661 cacctgcttg tccgacacag atcacgacat ctggttggtg ctgttggatg gcttctttga
     6721 ctacttgact gcttttttcg aatacggtcg gtacttccac tttgatgatt tcagcaccgc
     6781 tgatttcatc tggcattttt tttactgctt cataggctgg attcactttg tccccaccaa
     6841 atggatcaaa acctgttact agtactttca tctgttgttc ctccgtccga ttgatttaac
     6901 atatggttat cttttatcat accattttcc ttgaacagcg taaataaagg aaacaatagt
     6961 ttaaataaca taacagatca aacttaatat attcttataa aaaaatagtg ttccactaca
     7021 atgttagaag aggttgcgac aattattctg tcacaacccc tctcttctat tatttaaata
     7081 gaatcatata agcaatttgg aaaactagca taaataacgc aataaacaac tggtttttga
     7141 tcactccgta tttgtctttc atctccagca tcgctacagg cacgatattg aagttagcag
     7201 ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa tgcaaccatt cctattaacg
     7261 ctggattcgc tccatgacta aatacgaatg gtgcaccgat cccaaccgtc atcacggtga
     7321 tggccgcaaa tgcattcccc atgattaccg taaatatcac catgccaaga gcgtaaatga
     7381 tgatgccgat gttcacattt ccttctggaa cgatcgtacc tactgcattg gaaatcactg
     7441 tcccaactcc agcgcttgta aataccgcac ctagagaagc taaaagcatt ggcaacatgc
     7501 ttaacggccc tactgtgccc agcatatctg ctgcatcatc taaaaatgta cttggtcgat
     7561 tatctcgaga aaagaacatc aagattagca tggctgcaaa aacaccgacg cccacgccga
     7621 ctaaagcacc taaattggta aatagagcaa acaagatcgc aaagacaccg atactcaaag
     7681 ctggaataaa aattttcatt cctagtttgt ctgacatttt ttccatatat tcttttgatg
     7741 gaagccggct ttctcctttg ctgactttct tagcaattgc tggtatggtc atcgcaaaga
     7801 tcaagccacc gttcacgatt gccggaagcc atcttcctcc tgcaataact acccctaaga
     7861 ccgtccagaa aaaggctgac ccataacgat gaggattggt tttatctgtt aaatttctta
     7921 ctccagtgta aatcaatacg agtcccatca cgatataaag gatctccagt aatttatcgc
     7981 ctaataaaat atcaggattt gtaaaaaagt ccatcttatt gcccctttcc tggataatat
     8041 ttcttcgcta atttcttttc tttaagatag aagtagacac ttgccacaac taaagccgtg
     8101 atagccactg gaacttccac taaagcaagc tgtcccaatg ttacgtcata tcctagatct
     8161 ttcagtgttg attggactag caacgcccct gctcctccga tgaatagtac ttggccaaag
     8221 aaccagcaga cattttccat agctgaagcc atacctttta gttcttcttc atgcttagga
     8281 tggacttcta acccttttga ttcgatggtc cccaatgcca tcggtaaaat gattggacga
     8341 acaaatccag caactccacc aaaactcacg ttaaaagccg caaagatcat tcgaaagatc
     8401 ccataaatat ctacaatcat accagcagat actttcttga atcgcttgat caaggaagcc
     8461 gctgattctt tcagaccatt tctttcaagc gttcctgttg ccaacataat gatgataaaa
     8521 atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa gttctaacat tccttgaatg
     8581 ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca ttacgatcaa tatcgagtct
     8641 aatttaaatg caaacccgag aataacaatc aagacgccta aaaggctaag ataattttcc
     8701 atatattttc ctccataaaa tgacaatatt tttttaatta taaagcaaaa cacataaaaa
     8761 atatagttgt tttctcacga aatagacact atttttaaat atttttcgtt atttttttat
     8821 cacaaaaatt atcttactta ttgtaaggta aagctttttt taatttattg cccgataata
     8881 tatagaaata aggtctagtt actgtttttt ttaaatgatt aataaataac aacttctttc
     8941 tatgaaattg caaagcattc atcttctttt ccgctaacga tatatccgtc agtgcttggt
     9001 acactttact aaaaagtatg agattgccga tatctggata ggtgtgctat actacagaaa
     9061 aagatataca atctgccacc gggtaggata aagaggatga tcattccatt aggtctttga
     9121 agggatgatt ttcctctttt tgtgtatttt tttatagtta aggaggaatg taagtatggc
     9181 atggatggaa ttaatcatag caggaatttt ggaagtattc tggtcaacga tgatgaaatg
     9241 gagtgatggt ttttctaaaa tcaattattc tttttacaca gtgattggca tgattgctag
     9301 tttttacttt ttgtcaaagg caatcaaaag tttgccgatg agtctctctt atcctatctg
     9361 gacagggatt ggcgcagttg gttcggtcat aattggtgtg gtcttgtttc atgataagct
     9421 gaatctttcc acttggttct ttgtggggat gctactcatc agtatcattg gcatcaagat
     9481 tactagcggc cactagacac ttggaataag tactgatcag aaaaaataag cctttttatt
     9541 taatattcaa tttttttatg aaaaaataag aaataagtcc aatgacctag cgacttctcc
     9601 actcatcagg tgtatgcttg tactatctat agaaaaggag tgaatatttt tgaaaagtct
     9661 ttcggtccaa gaattatcca agaactttgg tcacaaacaa gccctcagcg atgtcagttt
     9721 tagcttatcc cctggcaaaa tcgttggatt agtaggtcct aatggcgctg gaaaaacaac
     9781 gatcatgaaa gctattttag gactgatcca ttactcagat ggaacaatct gtattggtga
     9841 aaatgagatt tctccctcta gtcataaagg gttggaacaa gtaggagcat tgatcgaata
     9901 cccgggtatc tatccattct taacaggcta tgatcatctg aagttattct ctgaaaccaa
     9961 cgatgtatct gctatcgaca ctatcgtgaa tcagctcaag atggaaaagt acattcacaa
    10021 aaaagcgaaa tcctactcgt tgggaatgaa acaaaaattg ggaattgctc ttgccttact
    10081 gaaccatccg tctatcgtta ttttagatga gccgatgaac ggtttggatc cacaagcaac
    10141 aagagatgtg cgaaaaatca ttaccgactt ggctactcag ggcacctctt tcttgatctc
    10201 cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag ctgatcctga tcaatcaagg
    10261 aaagatcatc aaacaatgta caatggctga attgaaccaa acagcgcaag atttcatcgt
    10321 tatctctaca acaaatgatc tagctgctcg aacgatcttg acagaagctg gatatacttt
    10381 ggaagatcct gataaaatca agcttttaaa agaaaacgaa gaattattag gaaccgtgat
    10441 ccagctattg tctgcacaaa atatccatgt gacagacgtc cagcatctga cgaatgattt
    10501 ggaatcttct ctattggaaa ttctggattc agtaaagggg gaagaagaaa aatgacattg
    10561 cttgtaaaac aagaattatt taaattgatc aagaaaaagt caactgctgt tttatctgtt
    10621 ctattagttg ttttgctcat aggaacagca cttctagcca aaaaatacac gacgatcatt
    10681 gatccagttg aaatgactgc tcagcttttt agtgccacat cttggattgt ctttatcatg
    10741 attgcagctg caagcacgat catttcgatg gaagcacaat atggtacatt gaaaaatctc
    10801 ctttatcgaa agtattctcg aggagaaata ttagtaagca aatggatcac attagttatt
    10861 tattctgttt atttatacct tttagctatt attgtcactg tgttgatgaa actgattctt
    10921 ttcccttcga tttccttcac ggagaaggtt tctactggtc aaacactgat ccaaagcttg
    10981 ttcacttata ctttgggtag ttatatcggt ttatggctga ttttaagttt agtgttgatg
    11041 attgcttgct ttatcaatag ttccggcgca tccatctcag caggaatcgt cttttatttt
    11101 gcttcgtcaa tcatatctgg tatccttatt gcactcatcc aaaaatggga gtggatcaaa
    11161 tggaatccga tcagtatgtt gaatctgcaa aaccaaattg gaaacgaaga gatcatgaag
    11221 caattgactc atctttctac taatcaaatg ctattcggta atttagctta catcgttttg
    11281 ttcttagcat taggatatct cgtatttaag cgaaaaaatg tatgatgact catcaaaaaa
    11341 gaggctgtga aagagacgtt cagccttatt gccgattggc tgtctcttaa acaccgttta
    11401 ttctgaaaaa ggagctgtga caacatttgt gtcacagctc ctttcctagt atgtttttga
    11461 ataaacgaca ggctcacaac ctctctttat acttctttta tacttctata cgtgaagcat
    11521 cccagtggca aataaaacca ttgcaatcac tccgaccatc gtcacaccta taagtaaaat
    11581 cttatctaag tggatatgtt gtacaggctc gttttgctcc ttacgtgctt tgaggtaaaa
    11641 ataaatgcct ggcaagaaaa gaacaagaac aagcattact tcattaaagg ctacgactag
    11701 catagcccat acttgaaagg cacttgctaa aagccctact aaaaattgga cccattcttt
    11761 ccgctgataa gaaagtttca tttggtacaa tcctaagaaa agccacgtga agaaaatagc
    11821 tgctgtacca agtgtgtaag caaagttata agcactgtca gtgaataata atgaaagtaa
    11881 aaagacattc gtacataacg tcgtcaatac gatagcataa gttggtgccc catttttgtt
    11941 gagttttccc catgctttag gcaaaagtcc gtcttcagcc atgtttccca ttgtttcaat
    12001 cggtaagatc gtccatgaga gccaaacacc aactaaagaa atgatcaaac cgccgttgat
    12061 cacgactgct ccccatttac caaccatcat ttcaaacaca tagcccatag caggctgcgc
    12121 cttgatcgtt tgcagctgat tcatcgtcat tgtcccatat ggaagcatgg aaaccaagac
    12181 gtaaataata atcaagccga atatccctag aatcgttgct ttcccaacat ctgattgttt
    12241 ttttgctcgt gaacctacga cagaggctcc ttcaatacca acaaataccc aaagcatcac
    12301 catgatgcaa ctcaatactt gactggaaaa cgggatcggc tgtccattcg cattcaatat
    12361 aatgtcgcta gaaaagttat caatgaattg ttggatcgaa aacgtgctga tagctactac
    12421 aataaataaa aataatggca ctaatttaca gaatgtgacg atagcattga cgaaactagc
    12481 attttcgact ccccgattca ctagccatgc atatccccat aaaaagactg tactcatcac
    12541 gatcgatgcc agattttgcc caccttcaaa tactgggaag aaatatccta atgcgctcat
    12601 caacaatgtt gcaaaagcta cgttgcctaa ccagcatgtg agccagtaac cccaaccgct
    12661 gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa ctaaagatcc cgccttttaa
    12721 atctggacgt tttttattga tattattgat cgacaaggag agcatcaaca ttgctgttcc
    12781 tacgagtacc caactgaaaa tagctgggcc gccagccgat ccggagatgt ccgtcataat
    12841 accaaatacg ccacctccaa tcgctgaacc aattactaag gcagcaagtg gaattaaacc
    12901 aattcctttt ttctgttgct ctgtatccat gtaagcatcc tccaaaataa aaccgctttt
    12961 atcaaaaagt ataacaaacc ttttttattt ttacttaatt aataaaaact ttaatagaaa
    13021 tataattcta ccggcttttt tctcattata caagcaaagc gcctagtccc tttccaaatt
    13081 acaccaccaa aaaacttaaa aaagatgcta tactaaaagg aaagaattgg aggatgatcc
    13141 atgaacattt tatatatatc gatttcagga aatacgcgtg cctttgccaa acatctagca
    13201 gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc ctgaagttac tttaaaagaa
    13261 atccatgaaa atagtgattt tacgaaagaa acagaaccat tttttacttt cgttcctact
    13321 tatttagacg gcggaaatgg tttagataac ggtgatacag aaatcttgac cgaaacgatg
    13381 cgtgaatatc tggaacatga agacaatcat aaactttgtc ttggtgtagt cggtagcgga
    13441 aataaaaact tcaataatca gtattgtcta acagccaaac aatatgcaca aacatttggc
    13501 tttcctttct tggcagatta tgaattacgg ggaacaccat ctgatgtgac acgaatttat
    13561 cagattttag cagaaaacaa taaataataa aaaagaagaa gctgaaaaat agatttcagt
    13621 ttcttctttc gtttctattg tttctatcat attaagcttt gacatccatc acaggtgctt
    13681 ttgcttttgc tttacctgtt ttttccaaga caaagttttt tacacgatcc atattggtta
    13741 ccaagacgat catggatgtt tctttgcctg ctgctttgat cgcttctaag tcagcttctg
    13801 ctacaagcgt atcaggtgtc acttgctgcc cttctgttac tttcacatca aatggcgtac
    13861 cgcctaagtc aactgtgttg attcccatat gaagcaagat ttctaaacca tttggcgttg
    13921 tgatcccaat agcgtgtttc gttgggaaga cagagctgac taccccagta actggagaat
    13981 agatatgtcc gttttctggt tctacaaagt agccttcacc catcatcttt tgtgcaaaaa
    14041 ctggatcact tgctttatct aaagaaacaa ctgttccgtc agcaactgat gagaggatag
    14101 tcacactttc agctgaagtt tgaggtacta ctttttcagg cacttcattt actgtggctg
    14161 tttcagtcgc taattcttct ttttctaaac ggtctttagg aataccgaag aagaatgtag
    14221 caacgaagcc accagcataa gctgccaata atccaaggat gtagcctaac catttaccgt
    14281 cagagatcaa tgggatcaaa gcggctcctg aaggtccaat agcgatagcc ccgacatttc
    14341 cgatcattcc gatcactgcc ccgccgatac ctccaccgat acaagcagta ataaatgggc
    14401 gtcctagagg taatgttact ccgtaaatca atggttcgcc gattcctagg atcccgactg
    14461 gtaaagctcc tttgatcatt tctgttaatt ctttatcttt tttgcagcga atccataatg
    14521 ccaatgctgc acccacttgt cctgcaccag ccatcgcaag aattggaagc aatagcgtac
    14581 gaccagtttc atcaatcatt tgcacatgga ttggagtcaa aatttgatgc agtccgaaca
    14641 taaccatcgg caagaataat gtgcctagaa cgaagccggc aaatgctcca ccaacgccaa
    14701 gtacccagtt gatcccaccg accatgccat cagaaatgaa acctgctaaa ggcatgatga
    14761 agaaaatttc aatcagaccg atggcaatca atgaaagagt tggtgtaacg atgatatcta
    14821 ccgcatcagg tactacttta tgcagttttt tctcaacgat tgacaatagc caaacagcga
    14881 agatgacacc taggatccca ccttgtccag ctgcaagagc ttcaccagta aataagtttt
    14941 tcagtggtgc ttccggattc atccctgtca gcatcacaac ggccccgata actccaccaa
    15001 gagttggtgt tgcaccaaaa acttgtgcag cattgatacc tgtatagata actaggtagc
    15061 taaacatccc gtttttcaag atgttcatca catcgatata ttgttgccat gttgctgcat
    15121 caagtgttcc ggctgtgact aagtttgcta aaactgcggc aatccctgcc acgatcccag
    15181 taccgacaaa tgcaggaatc attggcacaa agatattaga aatgtctttt aataccgatt
    15241 tgattttaga tggtttttgt ttcgattttt gtgccgcttt catttcttgt gcccgttcgt
    15301 ttacacggtc ttttccagat gctgatgaat ttgctgtagc tgtacttaca gaagaaagtt
    15361 tttctcctaa tttcacgcct gccaaatcaa ccatttgctg tgctactttg ttcactttcc
    15421 caggtccgat gatgacttga agctgttcat cttctactac accaagcact cctggaatcg
    15481 cttttaattc agagacattt acaagatcat cattgatgat cttcatacgc acacgagtca
    15541 tgcagtggac aatgctttct acattgccca tgccgcctgc ccctttataa atttgttttg
    15601 ccagttcttc caaagtcagt tcgttttttg ccatggtctt ttccccctca taaggtccct
    15661 tttacaaatc cgtttgcttc agctaatttt gcttgagctt cctctttatc actatttgtc
    15721 aaaatcatga cgatagccag cttaacattt tgatctgctt cttcaaagta cttttcagct
    15781 gtttcgtaat cagcttctgt cgcttgcatg atgattcgtt ttgagcgttc gattagtttt
    15841 tcatttgttg gtttgacatc gaccatcaag ttattgaata ctttacctat accaatcata
    15901 ctgatagtag aaatcatatt caagataagt ttttgtgctg ttcctgactt caatcgagta
    15961 gagcctgtca aaaattctgg acctgcatcg acttcgattg ggaaatccgc gtgtttactg
    16021 atttcggctt gtttattgca agaaatcgtt cctgtgacag caccgatcga ttttgcgtaa
    16081 tctaatccac cgatcacata tggtgtacga ccgctagctg cgataccaat aactatatca
    16141 ttttttgtca aattcagctt gattagatct tcttgcccta gctcataaga atcttcggcc
    16201 ccttcgacag ctacagtcat tgcttcttct ccaccagcaa ttagacctat gaccatttcc
    16261 ggttctacac cgaatgtcgg tacgcattca gcagcatcta acacacctag tcttccactt
    16321 gttccggctc ccatataaat cagacgtcca ccttttttaa aggactcaat cgtgttagca
    16381 atcactggtt cgatacttgt cagttctttt tcaaccgcag cggccacttt ctgatcttct
    16441 ttgttcatta gttgaacggc ttgagaaata ctcatctcat ctaatccaaa agtttcttgg
    16501 tttctgcgtt ccgttgttag gttctctaaa ttgattttgc tcatttttat cttccttctt
    16561 ttcttataaa ttgatagttc ataccagctt tgatttggtt gatcaatggt agctcttctt
    16621 tgataacttt agctactaca ttcacttttt catccgctgg caaatcatat ttggttaatt
    16681 ggatctcacc catatagcgc aaatactttt cgttatctaa agtaatagac cctttcatac
    16741 gcgtagctgt atttcttgct gggataggcg gaatctctcg aaaacgtgca tctacgcttc
    16801 gtatgacatc tcttgcatcg tcttgccgat tcgtatgctt ccctaatata tattcgtaaa
    16861 attcttcatc tacaggttcc gtatggagca agactgtctg ttctttttgg aaagaagcaa
    16921 attgctcctg aacgttttct gacagacctg catctccgat gtaaacaata tccgtgttac
    16981 ttgctaatag atctagagcg cctgcaagag gatggatccc tcgatgttct tccaaagttg
    17041 gcaatccttg atatagtggg cctctcaact ctgtatctcc tggtacaaaa ccttggacaa
    17101 ggagaccttg aattttgagc cattgatttt ttttctgata ccattctttg tccagccctg
    17161 tttcgggacg tggataataa ttatgccaag cttcaatctg tgagaaatca gcttctgctt
    17221 ctttcaattc atcgatatct ttaggcgtga tcgtgctagc attcaaactg attttcaatt
    17281 gatgtgacca gtcggcaatc tgctgattgg aaatatgata atccattctt aaacctgtta
    17341 caccaattgc tttcaaaggc gttagatctt ctaatgaaaa acccgctcgt tccaatgcct
    17401 ctcctgaaat atcgaccatc aattctagtt tattcttttt cgcttcgctt cctagttcga
    17461 tcaatcgctt ttgatatgcc gaaacatcat cttctggtat atgcatggaa gtgaaaatac
    17521 cgacaaagcc attggatgcc atttggcgga tatactgttt cttctcttct gacaaagaat
    17581 cgttcataaa tattgaaaag ccaaacatat tatccctctt tttctgtatc cgtcttcatt
    17641 tctatgacta gagtatatat ctaaagaaac aaaatttcaa tacttttatt aaaataatta
    17701 aatttcattt ctaaaggttg agtttatatt atcaaaagaa ttgtcataac ctaaaaaagg
    17761 cactgctaag caaaagcagt gccttgaaat attttattta aataacacaa aaacataatt
    17821 actcataaga aaccaattct gtattatcta ccatgaatgt aaagaacccc tcattaggca
    17881 tcggatgaaa ataagcattg atcactctta tcaaggcatt atggcaaact aacagataga
    17941 cattttcttc gtcttcgatg cattctttca ataaaggaac gatccgtgca tacacatcta
    18001 agactgactc cccatttggg aatcgtacag cgaacgctaa acgtgctttt tgaaagttct
    18061 catctttgct tggcagtcca tcataatccc caaaatccat ttctaccaat cgttcatcaa
    18121 cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc tgtatctctt gctcgttgaa
    18181 gaggggaatg gatgattttc gtgattggaa caggcaaatc tactaccttt tctgctagtt
    18241 tctgtgcctg catctgtcct ttttcagtca gcggcacatc tgcccgtccg caaactaatc
    18301 cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata aagcatgtga ttctctcctt
    18361 cgttggcaaa gttcccctta accatacctt gttgttcttg agattacaag ggttagattc
    18421 tctcaagaga aataatctca gtagatactt tatccaggaa ggtttgatca tgttccacta
    18481 gtaacatggc aggtttcacg ttcaagatca gttgttctaa ttgttcttga ttgaagacat
    18541 ccaaataatt caatggttca tcccatgtat atagttcagc tggctgtgat aaagatttag
    18601 ccaattccac ttttttccgt tggcccatac tcatctgctc gatcttgtta tgaaaaacat
    18661 ctctttccat cccaagcttt cggaggttgt tcaaaaatgc ttggtagtct acttggtttt
    18721 tctctgcaaa ttccgccaac gttcctcgat tgtcttcata attttggctt gcataactaa
    18781 tgctcagatg ttttggctgg tatttttctc ctataacctt gccgttgaat gcccccagaa
    18841 gataatggat aatggatgac tttcctgcac cgttaggacc tgaaatcgcc acccgctgat
    18901 gaggctcgat tgtaaaatga attggctcga ataacagatt ttcataccct aattgaagat
    18961 cttctacgct taaaagtcgc ttatggtgag acgcttggct attcatcgtc aaagaatcga
    19021 tatattcgat atcttttaat agtttttctt tctcgctgat ctgggtctcc atccgattca
    19081 cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc acccactgct cctttattca
    19141 ctcgtctaga ttcagtatcg atgaatccga cttgtttctt tgttttatct ccttctcggg
    19201 aacgagacca ttcggctttt tcagctgctg ttttctttag cctactgact tcttttttca
    19261 atttttcatt ttgagccatt tcaaattcat cacgaagttt tttctgttct tcatagatag
    19321 agaaattccc ttgataaagt tccagttgac ttttttcgat tgctaaaaca tggtccacta
    19381 cttcatcgat aaatccccgg tcatggctga ccacgatgaa gccttgtttt ttctttttca
    19441 aataagccgc tacttgtttt ctaccagaga tatccaaatg attcgttggt tcatcgatta
    19501 acgggaaatg agtgtcatcc acaaataaaa gtgccagtag gacttttgtc ttctctcccc
    19561 ccgatagtgt gctgaattct ctccataaga tttcaggatc tgtttgcatc aattggagct
    19621 ctctttcgat ttcccatatc tcaaaatccg taatatcatt taacacgtaa taggttaaac
    19681 gttctttatc ttttgtctgt tgcgggaaat aggcaaattc ttgctgatgg attacttgcc
    19741 cttgataagg tagtttgttt tgtagaatat tcagtaaagt tgtctttcct cgaccgtttc
    19801 gtccgataag tcctagtttc cattgtgtgt caaaatttag atttgcttgt tcaaataata
    19861 atgtgccttg gctgtcgtag ccgaatgtca gattttttat ttcgatttta gacattttcc
    19921 atcacctcgt aaaaaaagac cagtcctatt ttcagaactg gctaaagagg atgcaagggt
    19981 tggatacaca caaaaagacc catacatcga aaaaaccaag ccaatcccga aaatctgcac
    20041 actgcttcct tgtctccaca aggtacagtc tgaatgcagc tttcatcaat cgacttaatt
    20101 tttcaatgca cggatccctc actttgtcct atatttgaaa ttacgatagc ataaagaaaa
    20161 tgattttgtc aactacttcg ggaggatggt gaaaaatctg ctaagtgtct gtcctaagat
    20221 ttttgttttt tctaatgttt gcctgtgttc cattgcttct tctaaaccta ttggacccaa
    20281 atgaatactt aagacaattg ggagaaaatc agaaagtcct tctagctctt ggtctctact
    20341 tccgcaaata gcgattattg gttttcgata tttttttgca agcactgcta caccgtatgg
    20401 aacttttccg tggatcgttt gctgatcgat ccgcccttct cctgtaatga tgacatctgc
    20461 ttgttcgatc tttttttcca atccagtgat ttggctgact aaatcgaaac cagaaaccat
    20521 cttcgctccc aacaaaaaaa gggctccacc aataccgcct gctgctccac ttcctggaat
    20581 atcatctaaa tccacctccc agaaatcttt tatttgctga gcaatctttc ctgcttgttt
    20641 atctaagaaa gctagcgtcg tatctattcc tcctttttgt cttccaaatt gccaagcagc
    20701 tccttgttga cctgtatatg gattggtaac atctgcagcg ataatcaatt ttactttaga
    20761 aacaagaacg ttttttcgat caaaatgaag ttttttagta gataataaag gatttccttc
    20821 tgtgtaccct tctaaagaag cacccaagga ttggagtaat cctagaccac catcgctaca
    20881 cccgcttcct cctaaagtga caataatttg ttctacttct tgaagcagtg catctttaat
    20941 aagttctcct aaggcataac tggaagcaag tcggatagtt tgatccgttg gtttatcaag
    21001 tagatctaac cccaatgctt gtgcagattc gatgactgcg gtgttttttc cttgccaagt
    21061 tgtcaataaa tagctgattt ttcgctgacg gaataagaga tccatcccat caatggtacg
    21121 ccatgttccc ctgagcgcgt aatagattgc ttctgcagta ttttctccgc catctgcaat
    21181 cggcacattg atttttcttc ctttacgatc ttcccagcca tttagaactg cctgatttag
    21241 ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc gccaaaatat ccatctgtat
    21301 ccctcacttt cttcttaagt aagacacttt ttttcatttt tttcaaacaa aacaatgcta
    21361 ttttcttcca tttttctata tactaaaagg gaactcattg aataaaggag agaaaaaaca
    21421 tgaaaatcgc aatagctggg gccggagcaa tgggcagccg aatcggattg atgctacatc
    21481 aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc tcacattgaa gccattcgta
    21541 caaatgggtt gattgctgac tttaacggta aagaagtggt cgcaaaactg ccaatctatt
    21601 caccagaaga aatcatcgaa agcaacgaac acgtggactt gatcgtagca ttaacaaaag
    21661 ccaatcaatt agatgatatg ttttgttcga tccaatccat tattactgac aatacttatg
    21721 ttttgtgttt actaaatgga ttaggtcatg aagatgtact ggaaaaatat gtgccaaaga
    21781 aaaatatctt atttgggatc acgatgtgga cagctggtct agctggtccc ggaaaagtca
    21841 cattgttagg tgatggagaa atcgaactag agaacttgga accagaaggc gaagcattta
    21901 caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa tccgatctat agccataacg
    21961 ttcgttattc catttggcga aaagcttgtg taaatggaac attgaatggt ttatgcacga
    22021 ttttggattg taatattgct gaactaggcg cgcaaaaagc ggcagagagc atggttcgaa
    22081 caatcgtatc tgaatttgct tctatcgcag caaaagaagg aatcatacta gatcaagaag
    22141 aagtgtacca acatattgct tctacttatg atcctgacaa catcgggttg cattacccat
    22201 ctatgtacca agacctgatc aaaaaccatc gtttgactga aatcgactac atcaatggtg
    22261 ccatttggcg aaaaggacaa aaatatgata ttgctacacc atattgtgcc tttttgaccc
    22321 aattagtcca tgcaaaagaa ggaatccttg gcgcagaata aaaggctctc tgatgagaat
    22381 cagctgaatc agcagctggt tctctttttt taagcccaat aaaagatcaa aaagaaaaca
    22441 caactctcat gatgccataa gaattgtgct tgtttgtttc agtacataat ctcttcaaaa
    22501 tgtaggtttt ttatttgttt tcaacagagg agaggtcata ttcgtattgc tcataaatag
    22561 tggaatcgtt agtgataaat cgttttgccc aattttctgc atcataaaat tccaatagct
    22621 ctgataggat tggcttaaat ggtgtatgca gaaattccgg taattcatct aaatcacgtt
    22681 ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata atgagcaatc gcttgattat
    22741 ccgtcactag tttcttttga aaatacatgg attcaaatgt ccgcaaagtc actccttgct
    22801 gaccctcccg caacagttcc agaatcccgc gcgattcccc tgtttttttc aggtactctt
    22861 cataagtaag atatccaggt tgttcattcc ctcgatgtgg aagaatgaaa tagttggtcg
    22921 taacatctct ttttataaat tcctgcttat agctttctgc tcgttctttt ctcccattat
    22981 tcgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaattc atatgtttcg
    23041 aataaaatga actattatgt ttcagcccaa gttcttttgc ttccaaaaaa tcaaaatgat
    23101 aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg tgttctgctt
    23161 tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt aaatgggcgc acgcgtcgag
    23221 ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt gccaactgtt
    23281 gtctagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa acatctaaag
    23341 agcggatgga aggcaaaaaa taaagtgatt tattttttgc aactagcagt acgtcatttt
    23401 tgttcatctt ttccgcctca ctttctctgt cactttttcc tatgatagcc tgtttaaata
    23461 gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa tttaacattt gtatgaaaat
    23521 atgatgaaat aaatcattct tctattaaaa ttcgcctaaa agttttctga atagttgtaa
    23581 ttgtctgaaa atttgttatc attagtcatt gttaaatatt gctatttatt cttaatataa
    23641 attttgattg tgaatttaaa agaaagaggt catttatgga agataaaaaa ttgaaaaaac
    23701 aacttagctc cagacatatc accatgttag ctttaggtgg agcaatcggt gcaggtctat
    23761 ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt gcttttttga
    23821 ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt ttaagcggtg
    23881 gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct cattctgctg
    23941 attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct gaagctgttg
    24001 cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta tttgttttta
    24061 tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc gcagaaacag
    24121 aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt tttgctactt
    24181 atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag caattgactg
    24241 accatggtgg gtttactcct catggtatga agggaatcat cagttctttg cttgtagtta
    24301 tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca gatgatccta
    24361 aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc ttttacatca
    24421 ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc acgaatgtca
    24481 gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat atcgtcaatt
    24541 ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca tcttcaagaa
    24601 ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct gttttaaaca
    24661 agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat cttggtgtag
    24721 ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc ctttcgtata
    24781 cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca ctaagaaaag
    24841 gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga ttgattttcc
    24901 ttggtatcct gttcacgaac tccattgggg taactgtatt gactggcctg ctttacctgt
    24961 tcatttattt cagttatcaa aaaaagaatg atgcgttcgt attgacgaat gaatgaaatc
    25021 gatagaaaat tttattttat ttatgtggaa tagatggagc tattgaagaa aggagttgtg
    25081 acaaaagtct tgttgcaact cctttttccg aataaacagt ctcatgacct cttggtaaac
    25141 ggtgttcaaa aaccctctgt gcaaaataat tgattgttct ccatgctcat gacatgatag
    25201 aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa agtattggaa gtttttgttg
    25261 atgatcggtg tttcaacggt agttatgttt ggtatgatgt acttgaatgt ctatgcgctc
    25321 gatcatatct tcttcagtcg tacacgagta ttcatggcgt tgatgatggg tgcagtcatg
    25381 gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa acaaaaagat aaatgttatg
    25441 atcttagccg ctagtcttct tgtttttgcc ggttccttgt ttatggtacg aagccaaact
    25501 actgtcaacg acacagcttg gatgaaggcc atgattcctc atcattctat cgctatcctg
    25561 gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga agaaatcatt
    25621 caggcacaag aagaagagat cgacaaaatg aaaaaaatga tcaaagaaat ggaatgacca
    25681 ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta tcagcttcaa gaaataagaa
    25741 gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa aagctgccct gcatcaagta
    25801 ataagaacag ccaaacaaaa atagcttctc ctatttttcg tttggttagg cttattaccg
    25861 cagatgcaag cttttgaaca ccgtttatta agaggttgta aaatcagcgt tctgacctga
    25921 gtattagaga gacaaatgga aaaatagttc ctcatatttt gccatttttt gagctaatac
    25981 cgagggtcag ctgattgaac accgtttatc gagaggttgt gaaaaagtcg cttagtttcg
    26041 cgtattaaga aaaattttgt gaaatggctt tccacatttt tgtataaatt tggcttaata
    26101 ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg gattgtgaca aaagtcttgt
    26161 cacaatccct ttttatgttt ttattgttct aattgcgctg tcttgcttac tgcttcttgc
    26221 ggtttcacaa atttttttga gattccccaa cctgtgaagg cactgaccat cgaaaacaca
    26281 ggagaaagca aactgaaaaa tacgaatggc aagtaagtga gtgtaggaat accaaatgta
    26341 cttgctacga aacttccggc aattccccaa ggcactaagt agttgattac tgttccacca
    26401 tcttctagcg ttctacctaa tacagttgga tcaaatcctg ctttttcata agtttcttta
    26461 aatgcattac caggcagaat aacagatagg aattgttcac caataaaaat atttactccg
    26521 atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt tcgtcatctt tcttgatact
    26581 cgttccatca cggttgagat caagccaaat tcgatcaaca atccacctaa tgacaaagtt
    26641 agaataatca atgacacagt aggcatcata ctttcgattc ctccgcggct aagcagtgaa
    26701 tcgatttgtt ggtttccagt atttgaaaca aaccctgatt cgatgacccc agccaacttc
    26761 gttaaggaag tttgtgggtt ttgaataaaa atcattccta ctgctacagc gatattcaac
    26821 aaaatcgtta aaattgccgg cacctttttc catgcacaca gtaacattaa aaataaagga
    26881 atcagtgacc agattgaaat agtaaagttt gcttctagaa catcagtcac ttcacgaata
    26941 gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa gaaagaaaca
    27001 acaaatgccg ggacagttga ccacatcata tgcttgatgt gtttgaacaa atcggcatca
    27061 acaatagctg ctgctagatt tgttgattcg gataatggag acatcttatc tccaaaaacg
    27121 gcacctgaaa caatagctcc aacaaccagc gctggattta agcctagtgt tgtcccaata
    27181 ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac agcgctacct
    27241 acgattgcac acacaacaaa tacagaaggg acaaaccact ggacactgat caatttgaac
    27301 ccaatgacca ttagagttgg aatgatccct gcttgtatcc atacagcaat caatgaaccg
    27361 actaaaataa aaataaagat tgggatgatt cctggtttga tgccattgat gattccttcg
    27421 tgcagccaat ctgtcgggta tctttttatc actgcataca acatgaccat tccgatcgct
    27481 gctaagatcg tgatattagg tgagaccttt aatccgacaa cgccaatcgc aatgcctaaa
    27541 acaataacca ttaatacaag tgttgcttct aaaaaacttg gtgttccttt ttttcccatg
    27601 ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat cggattactc
    27661 cgccgcaatt catccgcgat tcctccttta taaaaataaa aagacctgtt ttaaactaag
    27721 gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca ctttcaaact
    27781 tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc
    27841 gaccaccgac tttctgaaca aaacacctaa ccgctactgc gtgaaaagtt gacttgtacg
    27901 aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt catttttctg
    27961 aaaattattt ttataacttt cacacacaag tttatttttt actagaaagt agcgccctct
    28021 tgtttttttc ttgctactat ttcttgatgt tcttgcttga ttctttctaa tagctttggc
    28081 tctttcaaca gatcaagagc tgtattggcg agtagttctg ctgcaatcgc aatagaagca
    28141 aggcctttgt cacttttagc agcagctttg aattcttctg tgtgtccggc aatatatgtc
    28201 tcagagattg ctaccgtagg ctggattgtt ggtatgactt ggctgacatt tcctacatca
    28261 gaagaaccga gacttgttct ttgttccgtt tctatttctt catctggaac acctgcttct
    28321 tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa cgatgacatc atcaactgca
    28381 ttttggaaca atccaaactc aaaagtcgtg cccgtactta tcgcagcacc tcgtacgata
    28441 ttttccactt tttcatatac tttatctaat gtttgccgat ttcctgcacg taaatagaaa
    28501 cgtccagcag cgtaatctgg aatgacgttg gcagctactc ctccgtctgt aatgatccca
    28561 tggatattga catcttttgg caattgcaag cgtagagcgt tgattccatt aaacacttgg
    28621 atcaatgctt ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg ggaagcgaca
    28681 ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt ctgtcgtttt cccataacga
    28741 aaagctggat ggacgcataa cgctacatcg acatcctcaa aaaaaccttc tcgaacaaag
    28801 ctgcctttag ctgatccgtt ctcgccccct tcttcccctg gggtgccata tacacggatt
    28861 tctccaccag tttcatcaat gaattgcttc actatgctgg cggctaatac ggagtaagta
    28921 ccaaacaaat tatgcccgca tgcatgacca atacctggta aagcatcgaa ttctgccaaa
    28981 aaggcaattg tgggaccggg tttattggaa atataacgtg catcaaaggc tgttcggtgt
    29041 ccagctactt ctttttctac ccaaaacctt tctttttcta attgtttaat caacgctgct
    29101 gaggaataaa actcatagtt acttacttca ggatgttcgt ggatatctag agcaagtgct
    29161 tgatattctg gtaaacgata ttggattaga tccttcaatt ggtttgttga gattgttgtt
    29221 gtcataaaaa attcctccta tgctagtcat ttttcttatt ttctcccgaa tgtttcccat
    29281 gcaggcacgc ttgatccttt tgatgttttc tcgattactt tgactgtgtc ttctgtttga
    29341 taagcgtcta ctattttttg atacgtttta ttgttttcat cttcttttct tgcaacgatg
    29401 atattcacat aaggacgtgc atggtcatcc actggttcta agaagatggc atcagaagca
    29461 ggattaaatc cagcatctac agctacaccg ctattgatca ctgaaatgtc tacatctgac
    29521 aaagcacgtg ctgtttggga agcatctaat tcagaaatgt ctagattttt tctattctcg
    29581 gtaatatcag aaacagtggg cgcttgtcct tttgccttat ccactttgat caatccagca
    29641 gtttgcaata acagaagagc tctgccgcca tttgtaacat cattagggat tgctactttt
    29701 gctccgtctt tgatatcagc tacatccttc aaagtgttag aataaattcc cagcggtgca
    29761 ttgacagtat ttccgatcga tactaaatca gttccgtgtt ctttattgta attgtctaaa
    29821 aagaattgat gttggaaaga attcaaatca atcgatccat ctgctaaagc cacatttggt
    29881 tgattgtaat ctgtaaattt cacgtattcc aaattaatgt tctccttttt caaacggtct
    29941 ttcacgttgt cccatacatc tgtatcttct ccaataatcc ctagttttac gatagtttct
    30001 tttttgtcag ttgtatctgc ttttccttgg ctacaccctg ccaaccaaat aattgctaca
    30061 aatgtaatga ctacaaaacg aataattttt ttcatttttt ctcctccact ttcaatgtgt
    30121 aattttttta atcagttgat tactgataaa ttgacttaaa aaaactaaga ttagaatcaa
    30181 taatgtagcc acaatcgtta catctgtttg gaatcgatta taccctctcg atatagcaag
    30241 attccctaat ccgcctgcac ctactgctcc agccatggta gtcagtccga tcacgttgat
    30301 gatagtcaac gccgagaccc gaatgatacc aggcaaacct tctaacaaat acacacgtaa
    30361 aatgataccg attggactgg ttcccatcgc ttctgccgct tcgatcaccc caggatcaac
    30421 ttctagcagt gcgttctcga tttgacgagc aaaaaacgga acaactccaa aaatcaatgg
    30481 cactaaagct gctttcgcac caatcgtcgt tccaactaag aaacgagtag tcaatgctaa
    30541 aagagaaagc aagataatga aagggatgga acgaatgata ttgatcagct tatcaagtat
    30601 ttcaaatagc agacggttag ctagtaatcc attagggcgt gtcactacta acgtcacacc
    30661 taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa aaagacatat atagtgtttg
    30721 cttcgtcgct tcgacaaatt catccggaat caaataaaca ttgggcaaat atgttttaaa
    30781 gaaatcagtc attttatctt cctccttcga tcactttgat acgactatct aaatcgtttt
    30841 tttgtaaaat atttattttt acaccagatt tgatcaaata atccaatgct tttttccgct
    30901 gtttggtttc tccagctaaa gtaacgatca aactccctaa cggcgtttct tgtaataact
    30961 ctacattgcc atataaaata ttcgctgtta cttgatattt gctgtacaga tgagcgatta
    31021 gaggttcgtt cgtctgattt ccaatataac ttaattccac cagccattta ttggcgatct
    31081 gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc ttggtcaaga tgagtagctg
    31141 ttcgaataaa atctttggtc aattcttctt caggattgct gaagatctgg atgctgctac
    31201 ctttttcaac gattcttcca tcttccataa ccgctacctt gttgcagatt tctttgacaa
    31261 cttgcatctc atgagtgatc aggacgattg ttaaacctaa ctggcgattc aattttttta
    31321 acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc actggttgct tcatcacata
    31381 ataatacctt aggttcattc gccaaagctc gtgcgattgc tactcgttgt ttttgtcccc
    31441 ccgacagttg tttaggataa gcagatgctt tttcttccaa accgacaagt tccagtagtt
    31501 cattcacttt ttgacgacgt tcctgtttcg attttccaga atactttaaa gaaaaatcaa
    31561 cgttgtcaaa aatagttcgg ctttccatta gattgaaatg ctgaaagatc ataccgatgg
    31621 cctttctttc ttttcgcaat tctttgggag agagctgact cagattcgtc tgattgacaa
    31681 tgacttctcc tgctgttggt ttctgcagca aattcatcac acgaactaac gtacttttcc
    31741 ctgcacctga gtaaccgacg attccataaa catcgccttt ttcaatcgtt aaatctacag
    31801 aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaaca tctcgcaatt
    31861 caatcattaa aattcctcct tatctaaaaa aagcgtcctc aagaaaatga aaatgtcatt
    31921 ttcttgagga cgcttttttg cgtgttacca cctcatgttc aagctgtgtt cacacacaac
    31981 tcctcttcaa gtacgatcat actctagcaa gatatcggtc gcagccgtca tcgccttaca
    32041 tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct tgcttcttct
    32101 cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc aatgcaaata
    32161 atattcaaaa aaaaagtcct tttactaaca taaactatgt cagtaaaagg acgacgtatc
    32221 gcggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca aaaagagaga
    32281 ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc tacttatcga caaattattc
    32341 tccaaggcca ttttcagctt ttcttacttg actcttctca ccagccagag cctctctgca
    32401 aagtttccaa acttactttc cttttcaacg aaatcgatat tttgttgttg ggattaatct
    32461 acatgaggaa aactgaaaag tcaagtacga aattcaaatt attttttcta tatttatttt
    32521 tctgtttttt tatttaacat ttcaataaca gttcgaagat cttttccttc cgcaagaagt
    32581 ttcaattttt ccttttcttc gacggtataa tttcggacaa aactggaatc aggtatcaaa
    32641 acaacgccta atcctgctcg tgaagcagct gttgctccta ctaaagaatc ttccactgca
    32701 aaggctgaac tagcttctaa tcctgttcgt tccaaagctg tcagataagg aatcggtgaa
    32761 ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt ccaagtaatc agctaattta
    32821 aagtgatcca agatatctgt tgctcttttc gtaacagtgg tcgtagcaat gcctagtttc
    32881 atatgacgtt cttttaagtc tgccaatatt tcttttgcat atggttttaa gtggatggct
    32941 ccgcttgtca gctgttgata aaaatattct tcacgcttcg ctcggacttc ctgtactttt
    33001 tcagggctac ctgctgcttt cactaaaata tcaaaacttt gtttccagtt ttttccgccc
    33061 caagcgtcaa cgatttcttt tgggatatgg atatcaaatt gctgaaatcc ccaaagccag
    33121 ccatcacgat acgttttttc tgtgtcaact aacaaaccat ctaaatcaaa tattgcacct
    33181 ttcatctata cattaatcct ttccaaaaca atacacatag tttagcacga ctggtccaaa
    33241 aagaacagaa gcaaagaacc agccttctcg tttataagag ttcttggaat cgacggatat
    33301 agattttctt cacagctgtc acaagcgcca gataagcgat gactgtagca aataaccata
    33361 accagtaatt gccaggaagt ggcatcaagc ctaattcagc accaaaactt gtaaaaggaa
    33421 ggacagaacc gacaataatt cctaatgtag tgataacaaa catggcaaat gttgcgttgc
    33481 tttggataaa tggtactttc ggtgtacgca gtgcatggag cactaaggtt tgtgtccata
    33541 gtgattcgac aaaccatcct gcatggaata atgcgatgaa aatcactcgc tgtgaactat
    33601 ccaatgtatg gaaacttccg cccactacag caggacaaat aacaaaatac atcaacaagt
    33661 aagtagtaat atcaaatatc gaacttgttg gaccaaagta aaccatgaat tccccaatac
    33721 tagatgcatt ccattttttt ggctcgtcca aatattcttt atccatccga tcccatggca
    33781 aggaaatgca ggaaacatca tagatcagat tcaaaaacaa aagttgtaaa ggcagcattg
    33841 gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa catattgcca aaattagaac
    33901 ttgctgtagc cttgatatat ttcatgatat tcccaaaagt ttctcgtcca gaaagaaggc
    33961 ctctttccaa aatcgtcaaa tctttttcta aaaggatcac gtctgcagat tctttcgcaa
    34021 tatccacagc tgtatctaca gaaataccga tatcagccac tttcattgca ggagcgtcat
    34081 tgataccatc tcctaaaaat ccgacagtat gaccattttt acgtaaagct gtcgtcaaac
    34141 gtgctttttg ctgaggattc aattttacga atacttcatg gtcttcagct acttgtgcta
    34201 attcatgatc actcatttcc gagattttct caccggtaac caattcattg atctctagac
    34261 ctacttgacg gcacactgaa cgtgtaacta attcgttgtc cccagtcaga acttttacag
    34321 ctacaccatg ttctttcaga gctttcaacg cttgtttcgt tgtttctttt ggtggatcaa
    34381 ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc tttgactgaa aattctccaa
    34441 cgacgcttgg attggtcttt tgtgcgacac caatcactcg taagccatct tcattaagtg
    34501 actgtacttt tgcaagtacg gcttcttttc gttcttttgt taatggtgta acagtaccgc
    34561 caagatcaat aaatttagaa atgttcagca tttcttcaat cgcgcctttt gtgatcattt
    34621 gtgttttacc agctggatca gcaaccacaa cactcatacg acgacgttca aaatcaaaag
    34681 gaatttcatc cactttttga taattgatct tatctgtatc taaagtttct gttgcttcat
    34741 caatgatagc cacatctaac aaatttttca gccctgtttg atagtagcta tttagatacg
    34801 catggcgcaa aacacgatca tcttcatttc cttcacaatc catatgatat tcaagaatga
    34861 ttttatcttg tgtcaatgtc cccgttttat ccgtacataa cacatcgatt gcaccaaagt
    34921 tctggatggc attcaaattt ttgatcactg ttcctttttt ggccatcaca cttgctcctt
    34981 tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg tgttagacca actgctacag
    35041 atagtccaaa aagaaaagct tctagccaat ccccttttgt aagcccattg atgacgataa
    35101 ctgctggagc catcaatgac ataaatttga tcagtagcat ggatgtttta tggatgccta
    35161 attcaaagct tgattgaatt ggtttttcag atagcgtttc tgctacatga ccaaatagtg
    35221 tttcatttcc aacagaaaca atgattcctt ccgcacttcc actgatgaca ttacttccca
    35281 taaagacaag attttcgtaa cttgtttcgc tttgctgatc gtcttcacat actttcgcat
    35341 gcttttctac tggatagctt tcccctgtca atgctgcttg ggaaatgaat aaatcctttg
    35401 cggacaataa acgcatatcc gctggtatca tatcccctgc tgaaagccga acaagatctc
    35461 cacagacgat ttcttccata ggaatctctg tgtactttcc attacgtttt acagctgccg
    35521 ttactttcac aagtgatttc agtttttcag ccgcttgatt ggaacgtacg gactgaatca
    35581 acgtcattgt cccgctgacg ataaccatag tgaagataat ggctacaccg aaaagatctt
    35641 tatctccagg agctgctaat acatattccg tgatgaatga aataattcct aatgcgatca
    35701 acactaatgt aaatggcgtg atgtatgctt tcaatatttc tacaatgaat ggtgtttttt
    35761 tgccatatga aatcgcattt tcaccgtaat tttctcttga acgttcgatt tgttcttctg
    35821 tcaatccatt tgctgatgca tggaatagtt caaataattc ttgtttcgtt tttccagcaa
    35881 aagctgcata gattttttgt gtatctttct ttttttccat ctcaatctct cctttgtgtc
    35941 caaaagacag ctgttctcgc tgcttttggt ctttagctat tcatcggatc gatagagaaa
    36001 aaactgaaag gggctgagaa cagatagata atagccctgg cctttcctct agatccttgt
    36061 ttttcgaatg actgtcatcc atttgttctc atctccttta ctaagtaatt ttaattcatg
    36121 acttccaatc atttttcgaa tagttatttt tccaattaga ccacctcctt tttatttttc
    36181 accgatgggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg aaaggtttga
    36241 aaaagcatat taaaaaaagc ttctcagatc tcatcgttga gttttgacac tgtacaacgt
    36301 aaaaagcaga tacctgcttt tagctacctt atgaaaagcc ttatctcgac aattcctgtt
    36361 ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat aggagcctca
    36421 cctaacaaat cttattcggt tttccttcgt taatataaca actaaaaaag aggatgtcaa
    36481 atcaattttc catgtttttc atactttttt taaaatacac acgttatatt cctgtctcac
    36541 gcggttttat ttgtctcatt ttccacctcc cgctataata gaaatatagt aggaaacgag
    36601 gtgctgttat ggaaagaaca cttgttatta tcaagccaga cggcgtgaga cgccatttag
    36661 tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa atgaagtttg
    36721 aaactatgac tcctgaatta gcaaaagaac attatcagca cttgactgag cgcagttttt
    36781 ttgatgaact gatcgattac atgacttccg gtcctgtcgt ttatcttgtg ctggtcggcg
    36841 aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac aaaagctgcc gatgctgttc
    36901 caggtacgat ccgaggagac tacgcgctgc cggggactga aaacatcatt cacgcttctg
    36961 attcccgtga tgctgcggta aaagagatcg cccgcttttt cccgccattc gatataaaca
    37021 aaccgtctga aaaagcggcg aagtgataca aattctaaac tgctggtcta gttgctgggt
    37081 tcagcaatgg ccagcagttt tttctctaag gacctccgtt gattctttcc taacatatat
    37141 tcgagcactg tgaagaattc ttggtagtct ttcgtctttt cttcccctaa agcagcagaa
    37201 atcaatcttt gataataatc tgccatcaat gtttcataga tcactgtact tctttcttta
    37261 tgtgctaagc tccgtttcat atcttcattc acactacgtg cctttttcaa ccgaccattt
    37321 tctatataaa acgaagtcag gttcattaaa aaagtaaaga gatactgctc atgattgttc
    37381 aactgtgcta atcgtcgcat tctttttttg attttcaaat aattgttctc aatgaaccga
    37441 tcactgaata tccaaggaaa atcagctaga atagatagtt caaattgacc atagtctggt
    37501 atctgcatca agtattcttc aatgattcgg cattcataac gtaaaaattc aaattctgtc
    37561 tcagatactt ctgttttttc gaccatgtga agattcaaaa ccaaataata cagatcttct
    37621 gttttcttaa aatgcctacg gttattgctg atctctttat aaggatcaaa gctgtcttct
    37681 tgtacccgat ctagcattcg tctttttttc tttaacttta ttggttcatc cagctggttt
    37741 cgcatccatt catattctgc aagtgtcatg ttcattcgct ctaaaaatga tctcaatgtt
    37801 acaaatgaca tttttttcac tgaatttgtc tcaatcccga ctagagaagt cttaaaacag
    37861 attccttccg ctaattggga ctgtgtcaac ttcttgttga ctcgaagttt cttgatcaat
    37921 tcaccatcat acataatagt tctccttttt aatttaaatt tttccacatt caataaatag
    37981 atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc ataaaaaata
    38041 ccaaccttca agcattagaa tgttgtctaa ctactgaagg ttggtttgat tgtttattga
    38101 ttattttttt gctgttcaac gtctgttctt gttactggat ataagttggt tgttgtttta
    38161 ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc gattgcttca
    38221 ttacgttccg cttcattttt atagtcatat tttttcggat caatcgtctc aaatccgttt
    38281 ggcacataaa accgcaagag attttcctga ttcagcttat ctgaaagact taacgcagtc
    38341 ttgacttttt cctgatcttt ggagatttcc tcatcgattc ctgctgcttt cgtatcgatg
    38401 atttctcctg tctgattatt gtaagctttt cctcctagga cggtgtattt gggtgtcaca
    38461 aagttcccat ttcgaaaagc aactacttga tcatgctgag aagataataa gtctgtacca
    38521 aaatgaagat aatctttatc atcgattccc aatagatgta gtaatgtcgg caaaacatcg
    38581 atttctccac cgtattcatg attgactgtc cctttagtat aaccaggcat atggatcatc
    38641 aatggcactc gttgcatctg tgcgttgtca aattcatccc atgtatctgg atctttacca
    38701 agagcgcttg ctaaatcttt attatctgta ttcgagatcc caaaatgatc cccgtaaatc
    38761 acaaagattg aattttggta aataccagat tttttcaaat acgtaaaaaa ttgttccaat
    38821 gattgatcca gataatgtgc cgttcgaaca taattatcta ctgtgctatt gccagtattc
    38881 aaagatggaa agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt ggttacggaa
    38941 agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga ttctctgaat
    39001 aaatctttat ctaatatccc ataacctaac gtttcgtcgg attcatcaaa ataagatcga
    39061 tcaaagaaat tgtcataacc taagttttta tatacatgat ctctattcca aaaactagct
    39121 acattcccat ggaaaacagc gcttgtatag ccttgctttt gtccaaggat cgctggtgcg
    39181 gcttgaaaga cattgtctga cccaagttct gtgaataatg agccttgagg taaaccaaat
    39241 gtgccagttt ccaacatatt ttcagcatca cttgtctttc cctgtcctac ttcatgaaag
    39301 aaattgtcaa agctgatcgt tgcttgattc tggaaaatac tatttaaaaa cggtgtgact
    39361 tcttgtccgt ctaccttcat attaatcaaa aactgctgga aactttctaa atggagaacg
    39421 atcacatttt tccctttagc gatcccaaat gtttcgggat ttggctctgc ataatgtttc
    39481 tttgtataat ctaaaacttc atcaatccca ttactgcttg catgcgcgcg aacactgcta
    39541 gtcattcctg ttttgattcc gtcataaata gtatacgcat cgattcctag atatttgaca
    39601 atatatgaac ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc gctcagcgct
    39661 agatttcctg aaaaaataag ccctgaaaga caggtaacag cgatagccat cggtttgccg
    39721 acagggttgc ttttgatagg tattttcttg accttcaacc aaaccagtag cccaatgaac
    39781 acagcgatat ccagccagta gatgacatca tggggcttca tcaaagaaaa ggagcttcct
    39841 gacaatcctt gcgacacttt cgaaaagcct aatacagatt tgactgtaat gaaatctgtg
    39901 aattcccgat aaaaaataat attcaagtac aacaaaagcg tatttaaaat atcaatgatc
    39961 aaaagaatag ctaacgctgg ctttggcttt ttaatataca aagaaaggcc aaaaaacaat
    40021 aaggtagtcg caattgggtt gatccacaag ataaagtact ggatcgtacc ttccacacct
    40081 aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa aacagctgtc
    40141 aaaacgattg ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta tatcacttct
    40201 ctctattcac attttttctt attttgctcc tgaaaaatga aaatttccta aaaaagttgt
    40261 tatcttaaga tttttttatt ctttctaaag attattccgt ccacttttca cagcataaaa
    40321 ccgctgcgat gtaagcgatt aaactaatga agagataaaa gtcttgtggt gaccgcttca
    40381 ctccacggta ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg ctgtgacaaa
    40441 attcttgtcg cagctcctta tttcttgatg caggacagct ttttcacaac ttcttcctta
    40501 aacggtgttc tcctgccaac tcctcggcca caagtcacaa tatttataaa acatgagaag
    40561 ctgttttttg aaattgttcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga
    40621 tacacggtgt tcaatcagct gaccctcggt attagattat gtcaggacgc taattttaca
    40681 acctcttg
//
LOCUS       DACELF010000014        38177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.014, whole genome shotgun sequence.
ACCESSION   DACELF010000014 DACELF010000000
VERSION     DACELF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.014"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            308..1192
                     /locus_tag="IW687_000724"
     CDS             308..1192
                     /locus_tag="IW687_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950008.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1401..1979
                     /gene="pgsA"
                     /locus_tag="IW687_000725"
     CDS             1401..1979
                     /gene="pgsA"
                     /locus_tag="IW687_000725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0950009.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2219..3460
                     /locus_tag="IW687_000726"
     CDS             2219..3460
                     /locus_tag="IW687_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0950010.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3569..4618
                     /gene="recA"
                     /locus_tag="IW687_000727"
     CDS             3569..4618
                     /gene="recA"
                     /locus_tag="IW687_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0950011.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5029..6585
                     /gene="rny"
                     /locus_tag="IW687_000728"
     CDS             5029..6585
                     /gene="rny"
                     /locus_tag="IW687_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0950012.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IW687_000729"
     CDS             complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IW687_000729"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0950013.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7611..10184
                     /gene="mprF"
                     /locus_tag="IW687_000730"
     CDS             7611..10184
                     /gene="mprF"
                     /locus_tag="IW687_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0950014.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10338..11189
                     /locus_tag="IW687_000731"
     CDS             10338..11189
                     /locus_tag="IW687_000731"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0950015.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11434..12372
                     /locus_tag="IW687_000732"
     CDS             11434..12372
                     /locus_tag="IW687_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950016.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12395..13075
                     /locus_tag="IW687_000733"
     CDS             12395..13075
                     /locus_tag="IW687_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950017.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13048..13872
                     /locus_tag="IW687_000734"
     CDS             13048..13872
                     /locus_tag="IW687_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0950018.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14039..14857
                     /gene="purR"
                     /locus_tag="IW687_000735"
     CDS             14039..14857
                     /gene="purR"
                     /locus_tag="IW687_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0950019.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15360..16733
                     /gene="glmU"
                     /locus_tag="IW687_000736"
     CDS             15360..16733
                     /gene="glmU"
                     /locus_tag="IW687_000736"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0950020.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16827..17798
                     /locus_tag="IW687_000737"
     CDS             16827..17798
                     /locus_tag="IW687_000737"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0950021.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17804..17906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17972..18541
                     /gene="thiT"
                     /locus_tag="IW687_000738"
     CDS             17972..18541
                     /gene="thiT"
                     /locus_tag="IW687_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0950022.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18622..19395)
                     /locus_tag="IW687_000739"
     CDS             complement(18622..19395)
                     /locus_tag="IW687_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950023.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19661..21361
                     /locus_tag="IW687_000740"
     CDS             19661..21361
                     /locus_tag="IW687_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0950024.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21331..21549
                     /locus_tag="IW687_000741"
     CDS             21331..21549
                     /locus_tag="IW687_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950025.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21569..22459
                     /locus_tag="IW687_000742"
     CDS             21569..22459
                     /locus_tag="IW687_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0950026.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22477..22761
                     /locus_tag="IW687_000743"
     CDS             22477..22761
                     /locus_tag="IW687_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950027.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23248..23862
                     /gene="gmk"
                     /locus_tag="IW687_000744"
     CDS             23248..23862
                     /gene="gmk"
                     /locus_tag="IW687_000744"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0950028.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23867..24172
                     /locus_tag="IW687_000745"
     CDS             23867..24172
                     /locus_tag="IW687_000745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0950029.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24425..26833
                     /gene="priA"
                     /locus_tag="IW687_000746"
     CDS             24425..26833
                     /gene="priA"
                     /locus_tag="IW687_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0950030.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26848..27339
                     /gene="def"
                     /locus_tag="IW687_000747"
     CDS             26848..27339
                     /gene="def"
                     /locus_tag="IW687_000747"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0950031.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27332..28270
                     /locus_tag="IW687_000748"
     CDS             27332..28270
                     /locus_tag="IW687_000748"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0950032.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28270..29628
                     /gene="rsmB"
                     /locus_tag="IW687_000749"
     CDS             28270..29628
                     /gene="rsmB"
                     /locus_tag="IW687_000749"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0950033.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29641..30381
                     /locus_tag="IW687_000750"
     CDS             29641..30381
                     /locus_tag="IW687_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0950034.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30378..32447
                     /gene="pknB"
                     /locus_tag="IW687_000751"
     CDS             30378..32447
                     /gene="pknB"
                     /locus_tag="IW687_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0950035.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32612..33511
                     /gene="rsgA"
                     /locus_tag="IW687_000752"
     CDS             32612..33511
                     /gene="rsgA"
                     /locus_tag="IW687_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0950036.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33524..34174
                     /locus_tag="IW687_000753"
     CDS             33524..34174
                     /locus_tag="IW687_000753"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0950037.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34175..34813
                     /locus_tag="IW687_000754"
     CDS             34175..34813
                     /locus_tag="IW687_000754"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0950038.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            34968..35822
                     /gene="mreC"
                     /locus_tag="IW687_000755"
     CDS             34968..35822
                     /gene="mreC"
                     /locus_tag="IW687_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0950039.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35826..36335
                     /gene="mreD"
                     /locus_tag="IW687_000756"
     CDS             35826..36335
                     /gene="mreD"
                     /locus_tag="IW687_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0950040.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36590..38128
                     /locus_tag="IW687_000757"
     CDS             36590..38128
                     /locus_tag="IW687_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950041.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        12552 a         6828 c         8305 g        10492 t
ORIGIN      
        1 acggaaaaat atctccttat attttgccgg tttttgagct aataccgagg aacagctgat
       61 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca
      121 agttcaaaaa acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc
      181 aggagaacaa tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca
      241 aacataacgt ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga
      301 tgaacgagtg gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa
      361 aatatcttta gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat
      421 cgacaatgat gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca
      481 atacgctgaa gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag
      541 caaattgaca ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa
      601 agcaatgcat caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt
      661 attaggattg gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg
      721 tcaagctgat ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga
      781 ggagtcctct tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac
      841 aacaaaatca agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc
      901 catcgcagta aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa
      961 tcgtgtatgg ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc
     1021 aaatgaaaca cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg
     1081 tgcagcaagc aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga
     1141 aaacaatcag agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa
     1201 attagtttac gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg
     1261 tcgcggtcct tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata
     1321 aattcatttg gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag
     1381 ggaagaaagg agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta
     1441 tgatcccgat tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag
     1501 gagacaccac actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta
     1561 tcacagactg gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca
     1621 agtttgcgga cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg
     1681 accaaggaaa ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca
     1741 caggcttgcg tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa
     1801 aagtcaagac ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat
     1861 tcagcctgat cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta
     1921 tctattcagg tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag
     1981 tatcgtttag tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt
     2041 caaaaaacag cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga
     2101 gaacactgtt tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct
     2161 ctttttaaat aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat
     2221 gaaagcagaa ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa
     2281 tgccactttc ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt
     2341 tgtaggagat aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga
     2401 actcatactc ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac
     2461 tgcggctcac ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc
     2521 atattttgaa acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat
     2581 cgaaggcggg gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac
     2641 agatacacat gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt
     2701 ggaacaagta cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa
     2761 agttttgcgc ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat
     2821 tgagacacag atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg
     2881 actgacagtg aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga
     2941 aaaaatccaa gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc
     3001 aaaagtcgta gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt
     3061 gacagccggc gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc
     3121 aggcggattc gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga
     3181 gctattagca gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc
     3241 acgtgagata gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac
     3301 gttagaaggt caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt
     3361 ttcggaaaaa tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca
     3421 aggatttgat cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg
     3481 gttttttctt gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg
     3541 atttgtaaat ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc
     3601 tgcactgaaa aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa
     3661 aatcgaccaa caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg
     3721 tgtcggcggt tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa
     3781 aacaacagtt gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt
     3841 cattgatgct gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga
     3901 tgaactactt ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt
     3961 agtatcaagt ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg
     4021 agcagaaatc gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc
     4081 tcaagcattg cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat
     4141 caaccaaatc cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg
     4201 acgcgcattg aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa
     4261 acaaggtaca gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc
     4321 acctcctttc aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg
     4381 agaacttcta gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc
     4441 ttataaagaa gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca
     4501 tcctgaaatg atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga
     4561 agtcgctgtc ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa
     4621 attatgataa acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt
     4681 tttattgaat aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa
     4741 tatctgttca tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct
     4801 atatgaaact agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt
     4861 ttctttgttt atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg
     4921 ttttttgtac acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga
     4981 agctgtatag atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac
     5041 attctcctcg ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag
     5101 tcacgtcacg agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt
     5161 gcaaaaaaag aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat
     5221 cagaaatacc gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct
     5281 caagagaatc gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa
     5341 aaacgcgaga actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt
     5401 gaagagcgag aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa
     5461 attgctggtt tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag
     5521 ctgaatcatg agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca
     5581 gatcgcaaag cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt
     5641 tcagaaacga ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt
     5701 ggacgagaag gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc
     5761 gatgatacac cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca
     5821 cggatgacct tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa
     5881 atggtcgaga aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca
     5941 gcgtttgaag tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg
     6001 cgattcagaa ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta
     6061 gctggtatct tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg
     6121 ctccatgata ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt
     6181 gcggaattag ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat
     6241 catggagatg tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc
     6301 tcagcagcaa gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga
     6361 agtctggaaa atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct
     6421 ggacgcgaag ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt
     6481 ttagtacgtg atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa
     6541 gtaatcgtta ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag
     6601 aagagccgcg gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta
     6661 tcattatttt ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat
     6721 gattgctggg atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc
     6781 aggagaagaa acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg
     6841 acaagaaccc ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt
     6901 cagtacacca gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc
     6961 agcaaggcca atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc
     7021 ttcaaaaatc atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat
     7081 cgcagaaacg cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc
     7141 ctgaggatct tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat
     7201 gaataagact ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg
     7261 tttcacggct aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc
     7321 atggacgcca gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa
     7381 cacctcttct ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat
     7441 attccctgtg ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct
     7501 ttctttttct atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa
     7561 gataacattg gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata
     7621 aacgatactt gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt
     7681 tcggctccgt gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt
     7741 ggcaagatat ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc
     7801 tggctggact gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct
     7861 tagaaaaaca aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa
     7921 caaacacgat caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta
     7981 gtttttatgg aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac
     8041 tatttatgct gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta
     8101 ttcagtcaga aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag
     8161 caccagtcat tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc
     8221 caaaaggctt gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg
     8281 tatttttgat catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta
     8341 tgttcatgat tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt
     8401 ttgacgtttt gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag
     8461 tttggctatt atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat
     8521 tgttcatcac acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt
     8581 ttttccaaaa ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga
     8641 tggtacttct atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat
     8701 tgccgttctc attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac
     8761 tcggccttgc tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt
     8821 tactatcttt tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg
     8881 tatatgtctt gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat
     8941 tcgtttattc ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg
     9001 tttacgcaat tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc
     9061 attttatttt gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct
     9121 cattgctagc tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca
     9181 gtgagtacga aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa
     9241 gccatcagct tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc
     9301 aagtcgtttt tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg
     9361 gcaatcctaa aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag
     9421 gctatcagct tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg
     9481 gttttcactt cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt
     9541 ctcggctgag tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt
     9601 ggtacgatca actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc
     9661 ttcaaggtgc acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat
     9721 ctagcggtgt cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc
     9781 cgatgacgaa gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg
     9841 aattagctga ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg
     9901 aagccaatct aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc
     9961 aagaaaaagt catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg
    10021 cttatgaaaa gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga
    10081 aaggaagtaa tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag
    10141 gaaatgttcc gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg
    10201 tttggattat gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact
    10261 aggcagacat aagctatact tttatttttc cctatgatag aatacttgcg acttagactt
    10321 ttcgaatggg tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac
    10381 tggatgtatt atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg
    10441 tagacttggc agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa
    10501 caaataaagc atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc
    10561 taaaaaataa atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag
    10621 tagcagctgg attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc
    10681 aattatggaa tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta
    10741 cagatgttcc ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa
    10801 tccaagtatt accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg
    10861 tctctacacg tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc
    10921 acggacttgc tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa
    10981 acagtttaga agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta
    11041 tgatcaagta cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct
    11101 tgtgtcaaca ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg
    11161 aagtttattt agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac
    11221 tgaggaatcc taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag
    11281 cagcaactca tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat
    11341 ggatttctat tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa
    11401 aattccgatt tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg
    11461 gtaatgatag gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa
    11521 aaagaggatt tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc
    11581 gtaggggatg aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc
    11641 gaaccaagtg caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt
    11701 tcagacatgg aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg
    11761 atgatcgaag cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa
    11821 gaatcagaag aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg
    11881 gtattagcta taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat
    11941 aaaaaggaaa cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat
    12001 caaaaatact ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct
    12061 gcttttgctt atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca
    12121 ccagatcagg aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac
    12181 aacatcaaag tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg
    12241 aatgaaacag gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa
    12301 atcaaagcag gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa
    12361 agtatcaatt aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt
    12421 gaccttttac tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg
    12481 tgaatttgtc attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc
    12541 gttaggatta ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg
    12601 aaaaaaatta agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc
    12661 tagtacggtg atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg
    12721 tctctctgaa aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga
    12781 tatgcgaaat cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc
    12841 gcgtattttt gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga
    12901 tgaagaatcc agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa
    12961 atcagttcta atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat
    13021 ccgactggtg agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga
    13081 gcgtttcttg ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg
    13141 atcagaagac aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg
    13201 ttaggattct ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca
    13261 gctgtgatat tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc
    13321 attttaatgg ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat
    13381 tcagcaacaa gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa
    13441 gtcatctttt taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg
    13501 ccgatgtatg ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat
    13561 ttgatgtcga tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt
    13621 gccggtgcac ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta
    13681 ggcaaaagct tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg
    13741 tcaggactaa ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc
    13801 tttattgctt tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga
    13861 aaaaataact aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct
    13921 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
    13981 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
    14041 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
    14101 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
    14161 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
    14221 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
    14281 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
    14341 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
    14401 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
    14461 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
    14521 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
    14581 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
    14641 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
    14701 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
    14761 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
    14821 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
    14881 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
    14941 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
    15001 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
    15061 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
    15121 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
    15181 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
    15241 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
    15301 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
    15361 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
    15421 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
    15481 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
    15541 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
    15601 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
    15661 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
    15721 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
    15781 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
    15841 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
    15901 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
    15961 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
    16021 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
    16081 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
    16141 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
    16201 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
    16261 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
    16321 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
    16381 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
    16441 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
    16501 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
    16561 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
    16621 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
    16681 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
    16741 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
    16801 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
    16861 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
    16921 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
    16981 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
    17041 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
    17101 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
    17161 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
    17221 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
    17281 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
    17341 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
    17401 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
    17461 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
    17521 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
    17581 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
    17641 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
    17701 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
    17761 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
    17821 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
    17881 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
    17941 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
    18001 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
    18061 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
    18121 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
    18181 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
    18241 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
    18301 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
    18361 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt
    18421 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
    18481 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
    18541 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
    18601 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
    18661 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
    18721 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
    18781 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
    18841 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
    18901 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
    18961 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
    19021 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
    19081 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
    19141 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
    19201 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
    19261 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
    19321 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
    19381 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
    19441 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
    19501 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga
    19561 tgttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    19621 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    19681 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    19741 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    19801 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    19861 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    19921 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    19981 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    20041 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg
    20101 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    20161 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    20221 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    20281 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    20341 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    20401 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    20461 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    20521 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    20581 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    20641 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    20701 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    20761 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    20821 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    20881 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    20941 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    21001 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    21061 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    21121 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    21181 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    21241 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    21301 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    21361 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    21421 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    21481 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    21541 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    21601 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    21661 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    21721 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    21781 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    21841 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    21901 ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    21961 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    22021 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    22081 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    22141 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    22201 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    22261 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    22321 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    22381 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    22441 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    22501 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    22561 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    22621 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    22681 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    22741 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa
    22801 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    22861 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    22921 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    22981 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    23041 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    23101 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    23161 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    23221 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    23281 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    23341 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    23401 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    23461 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    23521 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    23581 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    23641 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa
    23701 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    23761 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    23821 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    23881 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    23941 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    24001 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    24061 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    24121 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    24181 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    24241 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    24301 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    24361 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    24421 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    24481 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    24541 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    24601 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    24661 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    24721 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    24781 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    24841 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    24901 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    24961 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    25021 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    25081 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    25141 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    25201 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    25261 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    25321 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    25381 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    25441 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    25501 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    25561 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    25621 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    25681 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    25741 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    25801 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    25861 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    25921 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    25981 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    26041 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    26101 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    26161 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    26221 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    26281 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    26341 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    26401 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    26461 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    26521 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    26581 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    26641 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    26701 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    26761 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    26821 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    26881 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    26941 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    27001 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    27061 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    27121 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    27181 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    27241 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    27301 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    27361 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    27421 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    27481 agaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    27541 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    27601 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    27661 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    27721 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    27781 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    27841 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    27901 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    27961 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    28021 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    28081 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    28141 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    28201 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    28261 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    28321 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    28381 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    28441 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    28501 aaagtggatg actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta
    28561 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    28621 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    28681 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    28741 ctatggatga ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    28801 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    28861 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    28921 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    28981 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    29041 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    29101 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    29161 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    29221 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    29281 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    29341 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    29401 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    29461 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    29521 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    29581 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    29641 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    29701 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    29761 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    29821 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    29881 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    29941 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    30001 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    30061 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    30121 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    30181 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    30241 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    30301 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    30361 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    30421 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    30481 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    30541 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    30601 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    30661 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    30721 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    30781 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    30841 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    30901 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    30961 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    31021 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    31081 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    31141 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    31201 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    31261 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    31321 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    31381 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    31441 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    31501 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    31561 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    31621 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    31681 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    31741 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    31801 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    31861 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    31921 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    31981 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    32041 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    32101 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    32161 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    32221 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    32281 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    32341 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    32401 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    32461 tatatcagaa gggaatgcga cattgttttg ttgcattctc ttttttgtgt ttctatctct
    32521 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    32581 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    32641 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    32701 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    32761 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    32821 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    32881 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    32941 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    33001 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    33061 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    33121 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    33181 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    33241 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    33301 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    33361 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    33421 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    33481 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    33541 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    33601 cgcagattac atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg
    33661 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    33721 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    33781 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    33841 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    33901 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    33961 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    34021 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    34081 tgcaggggcg gatgtcttcg ttgcagggtc atatatctat ggagccgaaa atccagtcgg
    34141 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    34201 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    34261 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    34321 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    34381 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    34441 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    34501 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    34561 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    34621 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    34681 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    34741 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    34801 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    34861 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    34921 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    34981 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    35041 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    35101 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    35161 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    35221 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    35281 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    35341 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    35401 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    35461 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    35521 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    35581 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    35641 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    35701 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    35761 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    35821 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    35881 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    35941 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    36001 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    36061 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    36121 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    36181 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    36241 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    36301 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    36361 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    36421 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    36481 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    36541 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    36601 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    36661 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    36721 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    36781 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    36841 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    36901 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    36961 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    37021 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    37081 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    37141 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    37201 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    37261 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    37321 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    37381 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    37441 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    37501 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    37561 aacagtgcct gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac
    37621 agaagaatca acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg
    37681 aaacggcaca ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa
    37741 acctgtaaca cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga
    37801 agcttacaaa tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga
    37861 ctgctcaggg ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg
    37921 gacagttcct caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga
    37981 cttgttattc tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg
    38041 acaatatatc catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt
    38101 tgcccctgac tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct
    38161 ggactaactc ttttttt
//
LOCUS       DACELF010000015        37337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.015, whole genome shotgun sequence.
ACCESSION   DACELF010000015 DACELF010000000
VERSION     DACELF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.015"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..792
                     /locus_tag="IW687_000758"
     CDS             <1..792
                     /locus_tag="IW687_000758"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0950042.1"
                     /translation="NIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTE
                     TMRIEGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEM
                     GAEIIEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVF
                     ENRFQHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGI
                     TRVRNLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            805..981
                     /locus_tag="IW687_000759"
     CDS             805..981
                     /locus_tag="IW687_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0950043.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1116..1412
                     /gene="yidD"
                     /locus_tag="IW687_000760"
     CDS             1116..1412
                     /gene="yidD"
                     /locus_tag="IW687_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0950044.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            1596..2063
                     /locus_tag="IW687_000761"
     CDS             1596..2063
                     /locus_tag="IW687_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0950045.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2148..3032
                     /locus_tag="IW687_000762"
     CDS             2148..3032
                     /locus_tag="IW687_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0950046.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3149..3382
                     /locus_tag="IW687_000763"
     CDS             3149..3382
                     /locus_tag="IW687_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0950047.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            3470..3550
                     /locus_tag="IW687_000764"
     tRNA            3470..3550
                     /locus_tag="IW687_000764"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3504..3506,aa:Tyr,seq:gta)
     gene            3557..3628
                     /locus_tag="IW687_000765"
     tRNA            3557..3628
                     /locus_tag="IW687_000765"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3589..3591,aa:Gln,seq:ttg)
     gene            4143..4850
                     /locus_tag="IW687_000766"
     CDS             4143..4850
                     /locus_tag="IW687_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0950048.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(4910..5554)
                     /locus_tag="IW687_000767"
     CDS             complement(4910..5554)
                     /locus_tag="IW687_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0950049.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            5681..5968
                     /locus_tag="IW687_000768"
     CDS             5681..5968
                     /locus_tag="IW687_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950050.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6076..6396
                     /locus_tag="IW687_000769"
     CDS             6076..6396
                     /locus_tag="IW687_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0950051.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            6477..7790
                     /locus_tag="IW687_000770"
     CDS             6477..7790
                     /locus_tag="IW687_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0950052.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            7787..8506
                     /locus_tag="IW687_000771"
     CDS             7787..8506
                     /locus_tag="IW687_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0950053.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            8634..9185
                     /gene="raiA"
                     /locus_tag="IW687_000772"
     CDS             8634..9185
                     /gene="raiA"
                     /locus_tag="IW687_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0950054.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            9407..11941
                     /gene="secA"
                     /locus_tag="IW687_000773"
     CDS             9407..11941
                     /gene="secA"
                     /locus_tag="IW687_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0950055.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12067..13168
                     /gene="prfB"
                     /locus_tag="IW687_000774"
     CDS             join(12067..12129,12131..13168)
                     /gene="prfB"
                     /locus_tag="IW687_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0950056.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            13273..13959
                     /gene="ftsE"
                     /locus_tag="IW687_000775"
     CDS             13273..13959
                     /gene="ftsE"
                     /locus_tag="IW687_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0950057.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            13943..14836
                     /locus_tag="IW687_000776"
     CDS             13943..14836
                     /locus_tag="IW687_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950058.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            14988..16436
                     /gene="cls"
                     /locus_tag="IW687_000777"
     CDS             14988..16436
                     /gene="cls"
                     /locus_tag="IW687_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0950059.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            16773..17630
                     /locus_tag="IW687_000778"
     CDS             16773..17630
                     /locus_tag="IW687_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0950060.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            17745..18665
                     /gene="pstC"
                     /locus_tag="IW687_000779"
     CDS             17745..18665
                     /gene="pstC"
                     /locus_tag="IW687_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0950061.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            18665..19549
                     /gene="pstA"
                     /locus_tag="IW687_000780"
     CDS             18665..19549
                     /gene="pstA"
                     /locus_tag="IW687_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0950062.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            19564..20370
                     /gene="pstB"
                     /locus_tag="IW687_000781"
     CDS             19564..20370
                     /gene="pstB"
                     /locus_tag="IW687_000781"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950063.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            20386..21144
                     /locus_tag="IW687_000782"
     CDS             20386..21144
                     /locus_tag="IW687_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950064.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            21157..21834
                     /gene="phoU"
                     /locus_tag="IW687_000783"
     CDS             21157..21834
                     /gene="phoU"
                     /locus_tag="IW687_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0950065.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22072..23103
                     /locus_tag="IW687_000784"
     CDS             22072..23103
                     /locus_tag="IW687_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0950066.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23100..23867
                     /locus_tag="IW687_000785"
     CDS             23100..23867
                     /locus_tag="IW687_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0950067.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            23967..25535
                     /gene="liaX"
                     /locus_tag="IW687_000786"
     CDS             23967..25535
                     /gene="liaX"
                     /locus_tag="IW687_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0950068.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            25835..26152
                     /locus_tag="IW687_000787"
     CDS             25835..26152
                     /locus_tag="IW687_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0950069.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            26152..26502
                     /locus_tag="IW687_000788"
     CDS             26152..26502
                     /locus_tag="IW687_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0950070.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            26669..27607
                     /locus_tag="IW687_000789"
     CDS             26669..27607
                     /locus_tag="IW687_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0950071.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            27622..28452
                     /locus_tag="IW687_000790"
     CDS             27622..28452
                     /locus_tag="IW687_000790"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0950072.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            28496..29521
                     /locus_tag="IW687_000791"
     CDS             28496..29521
                     /locus_tag="IW687_000791"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0950073.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            29536..30474
                     /gene="galU"
                     /locus_tag="IW687_000792"
     CDS             29536..30474
                     /gene="galU"
                     /locus_tag="IW687_000792"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0950074.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            30599..31039
                     /locus_tag="IW687_000793"
     CDS             30599..31039
                     /locus_tag="IW687_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0950075.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31042..31644
                     /locus_tag="IW687_000794"
     CDS             31042..31644
                     /locus_tag="IW687_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0950076.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(31707..32810)
                     /locus_tag="IW687_000795"
     CDS             complement(31707..32810)
                     /locus_tag="IW687_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0950077.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            33020..34021
                     /gene="ccpA"
                     /locus_tag="IW687_000796"
     CDS             33020..34021
                     /gene="ccpA"
                     /locus_tag="IW687_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0950078.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            34376..34771
                     /locus_tag="IW687_000797"
     CDS             34376..34771
                     /locus_tag="IW687_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950079.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            34771..35022
                     /locus_tag="IW687_000798"
     CDS             34771..35022
                     /locus_tag="IW687_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950080.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            35065..35460
                     /locus_tag="IW687_000799"
     CDS             35065..35460
                     /locus_tag="IW687_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950081.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            35457..36713
                     /locus_tag="IW687_000800"
     CDS             35457..36713
                     /locus_tag="IW687_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0950082.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            36725..37048
                     /locus_tag="IW687_000801"
     CDS             36725..37048
                     /locus_tag="IW687_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950083.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            37041..37307
                     /locus_tag="IW687_000802"
     CDS             37041..37307
                     /locus_tag="IW687_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950084.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12550 a         6368 c         8066 g        10353 t
ORIGIN      
        1 aatatcatga tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc
       61 gaaccagaaa tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga
      121 gctggtactg aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca
      181 atcgtacaag atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc
      241 aatgtgctga ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc
      301 gaaatgggtg cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat
      361 atcttaccta cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct
      421 caaatgacag ctatccaatt agttgcagaa ggtacaagtg tagtgactga aacagtgttt
      481 gaaaatcgtt tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac
      541 ggaaatgtag caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca
      601 gacttgcggg cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt
      661 gtgcgtaact tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa
      721 ttaggtgcag acgttgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa
      781 acgattgctt aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata
      841 aagattttag tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga
      901 tatggtgtca tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat
      961 attcgagact tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga
     1021 gccatgaagc agaacatgac aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc
     1081 ctttttttag taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag
     1141 ttagaggata tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa
     1201 cctgttccaa ttatatgata aaagcaattc aaacccatgg tgcactaaaa gggacggtaa
     1261 tggggactgc acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac
     1321 cattgacatt ccaattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg
     1381 ttgtgaaaaa tacgagtagc actgagcgat aaagaaacaa tttggaaaat agcttcttat
     1441 attttgcaaa attgtgagtt attgccgaag tgttggattt tgaacaccgt caaaaaaagg
     1501 ttgtgaaaaa tacgggtagc actgagcgat aaagaaaaag aggaaaatta ttgtcaaagt
     1561 aacaactccg caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag
     1621 atttaccatg gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg
     1681 tttccagagg gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct
     1741 atgtggcaag ggaaaaagaa tacgaaatcg tcaatgtagc agtagcccca acgtatcaag
     1801 gaaaggggat tggcggaaaa ttacttgaga cagcttttca gaagctttca caactcgctt
     1861 ccagtcaaac tcagatcatc atacgaaccg gcagtactag cagtgcggcg ttacatctct
     1921 atcaaaaaat gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact
     1981 ctgagcccat ttttgaaaat ggagatcaat taagggatca agtaatttta gcaattcact
     2041 tacctaaaaa tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa
     2101 atagatacat tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag
     2161 tttttgattt tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag
     2221 gtaagggaca atttgcaacg cctgaatctt gggaagaaat gaaagcgcaa ttatttcaag
     2281 tttataaaaa aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag
     2341 tagtgaataa tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat
     2401 ttaatatttt ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg
     2461 ccaactgtgc cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg
     2521 cttttgttgt ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaatcaata
     2581 aaggggcaca tctttatggt ggagaatttg ggttgatgtt cttaaatggc gataagacat
     2641 tcagtaaact tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg
     2701 ataaaaattc ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag
     2761 caaaagaaga agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat
     2821 tttcgtttga tccagaagtg atcgtattag gcggcggtgt ttctgcaaaa gaagggctca
     2881 ttgatgagat caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg
     2941 aaccgcagat tttattatgt gaatacagaa acgatgctaa tcttgtaggt gcagcagcaa
     3001 actatctcgc tgtacgtcaa ggggagaatt aagagcttta gagaaggtgc agcttgcata
     3061 aaagaagtga atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt
     3121 atgataaaat atctgagagg tgaaaatgat gtcaaaaaaa aataaagatg ttgaagtgcg
     3181 tatagaagaa gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat
     3241 agggaaaaaa atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt
     3301 tggtgaagaa gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg
     3361 acactggaat ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa
     3421 atctggaaaa ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc
     3481 gaagtggcta aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca
     3541 ctctcctcca tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     3601 tgcgttggtt cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga
     3661 gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgatt taagatagag
     3721 ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag
     3781 cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa gtgaatattc
     3841 cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa
     3901 aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga
     3961 ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata
     4021 ggagtaggaa gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt
     4081 ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg
     4141 ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg
     4201 aacaaggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat
     4261 tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct
     4321 tagaaagaaa gatcggttcg ggtacatatg ttgcgcgaga aaaagtacaa gaaaaaatgt
     4381 ctggtgcaac aagttttaca gaaattatgg aatcgcaaaa tcgagtaccg tctagtcgga
     4441 caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta
     4501 aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt
     4561 ttgaagttgc cagtgtgcca caaaagctga tcgatggcta cagcaaagca gagattactg
     4621 aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa
     4681 cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag
     4741 atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg
     4801 tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg
     4861 atccctgatt ccttacttag aaataaaaga gaatcagaat cacctgtttt tatatatgat
     4921 cagttaccag ttgctcgtga taagaaacag gaccctcttc aaattggact tgtccgttca
     4981 gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca gcacagatga
     5041 cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt tcaagataat
     5101 tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt tgcaaggtac
     5161 cttctacgat tccaatgtca gacaatgctt gagagacagc atgagtgtat gctcgtatta
     5221 atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca gctacattga
     5281 tcagttgatt ttttttgagg acttccaaca tcggtacgcc agccgttcca cttggttctc
     5341 catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg ctgcagttat
     5401 ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct tggtcctctg
     5461 attctattcg tctaagagaa cagataaagc gtgatttttt gatctcaatc tcactttgtc
     5521 cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt ttcattttct
     5581 ttattcagta taaataaagt tctaggttgt ttcaacactg taacggttgc gcaatcgctg
     5641 tagtatgcga taataaagac aaaggtaggt ggatctgggg atgcaggaac gaatgaaaaa
     5701 gtacgatgca attacgcatt atttgaagaa caatggcggc tctcaagtaa cgttgacctt
     5761 tacccaattt gacgaactgc tgtttcccag taacggactt cccaaaacag caagggaaag
     5821 tacagattgg tgggcaaatg attataaaca ccctgaaaaa ggtgcctatg gctggataaa
     5881 tgcaggctat gaagtagttg tcatcaattt ggacaaagaa tatgtcgtgt tcaataaatt
     5941 agtgaaatct agttggctat ttgattaaat aaaccgcgtt tagaaaacgt tgacaaattt
     6001 ctttcttttt atataataga gtaacagacg cgaagaaaac gtttgcgtag tttgttttaa
     6061 aaaggagaga aaagtatggc taaaaaaaca atcatgcttg tatgttcggc tggtatgagc
     6121 acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag caaaaggaat cgactcggat
     6181 attttcgctg tgtccgcttc tgacgcagat aacaatcttg caaataaaga tgtagatgta
     6241 ctgttattag gacctcaagt tcgctttatg aaagctgatt ttgaaaaacg tctggaacca
     6301 aaaggcattc ctttagacgt catcaatatg gctgattatg gtatgatgaa tggcgaaaaa
     6361 gtgttagatc aagcaatcac gctcataggt gaataaatag aacagtataa ctatcgaaca
     6421 agcgatccaa aaaatagatc gcttgttttt tcgtatattt ttttagggcg aataatatga
     6481 atcatttttg gggtagaagg cttctgacga gagatattaa agcaatagac tgtgaggagg
     6541 taaatttacc aaattatgag aagatcgcag cagtggaacg tttgggcaac tggattcttt
     6601 gtcatcgctg tggaatgagg acgtccgtca ttgatggaca gcttgcagat tacggttatt
     6661 tttgtacaca ttgtctatat ttagggcgat gcgacactca acaagattta tatttgtttg
     6721 atcagcctga agcaaaacct cgagaagtcg tctttttttg gacaggacag cttactgaaa
     6781 aacaaacgga aatcgcaaag agaatacttt gccattcaga aaaaagacat catcttattt
     6841 gggctgtaac aggtgcaggg aaaacagaga tgctttatcc tattttagtg gaaactttaa
     6901 aagctggagg aagagtagcg atttgtacac cgcgagtcga tgtttgtaat gaattgtttt
     6961 tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt gctccatggg aatacagcaa
     7021 cggcttatac ctttacttct tttgttatct gtacgattca tcagctatac cgtttttatc
     7081 ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt tccttataac ggggatgcag
     7141 gattagctca tgctgtgcaa acagcgatta aaccagacgg acgattcatc tacttaagtg
     7201 ccacacctga taaaaagtta ctaaaggaaa tcaagcagac gtttgacatc cacaaacttg
     7261 ctttgcgctt tcatcggcgt ttacttcctg aacccgtctt ggttttcatg aataattggc
     7321 gacaaaaatg caagaacaag aagaagataa aatctttggt gcggatcatt catcaattaa
     7381 ttaaagaaaa ccatatcctt ttattttgtc caagtatcac gatgatgcat cgtttgaaag
     7441 ccacacttca agaggctctt ccgagatatg agatcacaga tgtttcttca agagacaatc
     7501 agcgggcgga aaaagtccag aaaatgcgag aagcaaaata tgacatactg ttaaccacga
     7561 tgatccttga acgtggagtc acatttgaaa gaatatccgt tatcgtttta ggagcggatc
     7621 atccggtttt tactaaatct tctttggtac aaatcgcagg aagagccgat cgtaaagggc
     7681 cttatacgaa cagtcaagta tactttttct atgaagaaaa aactgcagct attcgaaaag
     7741 cttgtcagga aatcaaagaa atgaatgaag aagggaagaa gatgctgtga ggtgcagttg
     7801 ctgcagtaaa gatatcgtcc aaaatttaac attcagcgag attattggtg tttcgaaata
     7861 cagattacct cagacttgtt cagtttgtcg cgcacaattt catttattgg aaacaagagg
     7921 ctgttctggc tgtcaaaaac caatccattt caatctagag agtacgaaaa agaaagtaaa
     7981 gtctattccc gtttactgca gtgactgtca agaatggaag aagaaatatc cgacctatga
     8041 ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg aaggcctggc tcaatcaata
     8101 caagtttatg ggagatatcc gactagcagg cacctttgca tataaatgga cgcagataaa
     8161 aaaagaatac ccgacataca tctattgtcc aataccttta tctgaaaaaa ggcgaacaga
     8221 aagaggattc aatcaagtaa gcgagatgtt gaaagcagca agaattcctt ttcagatgct
     8281 actcaaaaga gagaagcatc tgctggctca agcaaaaaac acgcgaaagg aaagattgga
     8341 gatgcctcaa ccttttgaac ttgcagtcac cttttctaag atacaaaata aaaaaatatt
     8401 gattattgat gatgtctata caacaggaca gactcttttc cacgcagcag attgtttgca
     8461 ggtcgcacag ccgaaaatca tccgaaccat ttctttagca agatgaatta gatgtggaaa
     8521 tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat taagaagagg
     8581 gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt tttatgttta
     8641 gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac tacgttgaga
     8701 aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg gcgcatgtga
     8761 acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca ttaccttatc
     8821 ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat ttggttgttg
     8881 acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa tcccgtgaaa
     8941 cgggcatgaa tactgcagat gctactgctt tattcaatga agaggacgaa aacgaaaatg
     9001 attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg gatagtgaag
     9061 aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa gatgcagaaa
     9121 caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg attgaaacag
     9181 actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac agctgccgca
     9241 agtttttttg agtttcctcc tcttttagat gaaatatgaa taaattatga agttcttact
     9301 ttaatacgct taagatattt caattatcta taactaatga taaaatgaaa agtaaagtct
     9361 acatagtctg gataatacac agaaggaaaa ggagattaca caacgcatgg ccaatttgat
     9421 tcgttcactt atcgaaaacg ataagaaaga actaaaacgt ttagatagca ttgcaaaaaa
     9481 agttgaagca tacgctgacc aaatggcagc attaactgat gaacaattac aggcaaaaac
     9541 tgctgaattc aagggcagat accaaaaagg agaaacatta gatcaattac ttccggaagc
     9601 ttttgcagtc gtccgagaag cagcaaaacg tgtattgggg ttatatccac accacgtaca
     9661 attgatgggt ggaatcgtgc ttcacgatgg aaatatccct gaaatgcgta ctggtgaagg
     9721 taaaaccttg accgcaacga tgccagtata tctaaatgct ctgactggcg aaggggtaca
     9781 cgtagttacc gtcaatgaat atttagcaac ccgtgactcg acggaaatgg gcgaacttta
     9841 taatttctta ggattaacag tggggttgaa tatcaactca aaatcttcgg atgaaaaacg
     9901 cgaggcttat aattgtgata tcacttatag tacgaataat gaattaggat ttgactactt
     9961 gagagacaat atggtcgttt accgccatca aatggtacaa cgtccgctga attatgccat
    10021 tgtcgatgaa gttgactcaa tcttgattga tgaagcgaga acgccgctga tcatttcagg
    10081 gcaagcagaa aaatccacag ctttatatac aagagcagac aacttcgtga aacgtttgaa
    10141 agaagaagaa gattataaaa tcgatgtaca atcaaaaaca atcagtttaa ccgaagcagg
    10201 gatcgaaaaa gcagaagaaa cattcggttt ggataactta tacgacattg aaaatactgc
    10261 attgacccat cacttggatc aagcattgag agccaactat atcatgcttt tagatatcga
    10321 ttatgttgtc caagacggaa aagtactgat tgttgaccag ttcactggtc ggatcatgga
    10381 tggacgccgt tattcagatg gacttcacca agcaatcgaa gctaaagaag gcgtagagat
    10441 cgaagacgaa acgaaaacca tggcgacgat cactttccag aactatttcc gtatgtacaa
    10501 gaaacttgcg ggaatgactg gtacagctaa aacagaagaa gaagaattca gagaaattta
    10561 taacatccaa gtataccaga ttccgacaaa tcgtccggtt atccgtgatg atcgtccaga
    10621 tttgctttac ccaacattac aaagcaagtt ccacgctgtc gtgcaagata tcaaagatcg
    10681 ctaccgcaaa ggtcaacctg tgttagtcgg aactgttgcg gttgaaacat ctgagttatt
    10741 atcagatatg ctgaatcaag aacgtattcc tcatgaagtg ctgaatgcga aaaatcactt
    10801 taaagaagca gagatcatca tgaatgctgg tcaaaaaggt gctgtgacga ttgctaccaa
    10861 catggctggt cggggaacag atatcaagtt aggacttggg gtaagagaac ttggtggtct
    10921 agcagttatc gggacagaac gacatgaatc tcgtcgtatc gataaccagc ttcgaggacg
    10981 tgcaggacgt caaggagacc ctggtatgtc acagttctac ttgtcattgg aagatgattt
    11041 gatgaaacga ttcggttcag aacgaatcaa atctttctta gatcggatga agatcggtga
    11101 ggaagatgct gttatccaaa gcaagatgct gacgaaacaa gtagaatctg cacaaaaacg
    11161 tgtagaagga aataactatg acacacgtaa aaacgtctta caatacgatg atgtgatgcg
    11221 tgaacaacgt gaagtaatct ataaacagcg acaagaagta atcatggaag aaaaaagctt
    11281 atccaagcca ctaatgaaca tggtcaaacg gacaatcagt cgtgtagtag atgcgcatac
    11341 acaattagag aaagaaaatt ggaatttgga aagtatcatc gattttgccg gcagtactct
    11401 tgtccatgaa gatagtatta ctcttgcaga tatcgaagga aaaacagctc aggagatcaa
    11461 agagtatcta aacgaacgag cacaagaagt atttaatacc aaagcggctc aactaaatgg
    11521 tccagagcaa ctgttggaat ttgaaaaagt cgtgatcttg cgtgttgtgg actctaaatg
    11581 gacagaccat attgatgcaa tggatcaatt acgtcagtca atcggcttac gtgcttatgg
    11641 acagaacaat ccattagtcg aatatcaaac agaaggctat aagatgtttg aagatatggt
    11701 aggcgccatc gagtatgaag tcactcgaat ctttatgaaa gctgagatcc gtcaaaacgt
    11761 acagcgtgaa caggtttctc aaggaacagc ttctcactca gaagacggag atgccaaaga
    11821 aaatagtaat actagcgcga aaaaacagcc aatccatgtg aacaaggtcg gaagaaatga
    11881 tccttgtcct tgtggaagcg ggaaaaaata taagaactgt catggaaaaa atgcaaacta
    11941 atttctgaca gcaggattgg tgagacaaat tcttttttta agaattcgtc tcaccttctt
    12001 tctgttattt gctgattctg ttagactaga tataatgaaa aaagtatagt gaaagaagga
    12061 aaaaacatgg aaatatccga aattcgaaat ctcctgacag aaatgcagga aaaggtcaca
    12121 agcttcagga ggtctctttg acttagacca gctggaagaa gacattgcgg aagcagaagc
    12181 aaaaatggct gaacctggtt tttgggatga tagcgaagct gctcaaaaag tgatcagtga
    12241 aaacaatgca aataaagaaa cttatgatca attccattct cttgctgaag aactggaaga
    12301 aatcgaattg atggtggaga tgttgcagga agaacctgat gcagccatgc aagaagaagc
    12361 agaaacacgt atcaatgcgc tagatgaaaa aatgaagaca tatgaattat ctatgttgtt
    12421 agatggtcct tatgatcgaa acaacgccat cgttgaattg catccagggg ctggcggaac
    12481 ggagtcccaa gattggggca gtatgctttt acgtatgtat acacgctggg cagagcagca
    12541 tgggtatcaa gtagagacac tagattatca agcaggtgat gaagcaggaa tcaagagtgt
    12601 gaccctactg atcaaaggac acaacgcatt tggttatctg aaatcagaaa aaggtgttca
    12661 tcgccttgta cgtatctcgc cttttgactc agcgaaacga cgtcatactt ctttttgttc
    12721 ggtagatgtg atgccagagc ttgatgaaac gatcgatata gaaatcaatc cagatgattt
    12781 gaaaatcgat acgtaccgag caagtggtgc aggtggtcag cacatcaata aaacagaatc
    12841 tgctgtacgg atcactcact tgcctacagg aacagtagta gctagtcagg cacaacgttc
    12901 acaattgaaa aaccgtgagc aagcgatggg gatgttaaaa gctaagcttt atcaattaga
    12961 agtggaaaag aaagaacaag aagcagctgc acttcgagga gaacaaaaag aaatcggctg
    13021 gggttcacag atccgttctt atgttttcca tccatattct atggtaaaag accatcggac
    13081 aaacgtagag acaggaaatg tccaagcagt tatggacggt gaccttgatt tatttattga
    13141 cggctattta aaaatgcatc tacaataaga acccattgtt aaattgaaat aaaattgtaa
    13201 gattttgcaa gcatgttgaa acattgccat gctataatag ctaaagactg aaaaagatat
    13261 ggagagaacg ccatgattga aatgaaggat gtaatgaaga agtactcaaa tggtacgacc
    13321 gctatccgca atctctcaat tgtcatcgaa caaggagagt ttgtctatgt ggttggtccg
    13381 tctggagccg gtaaatctac attcatcaaa ttgatgtatc gagaagaaaa agcttcaaaa
    13441 ggaactttga acgtcgcagg tcatgatcta atagcaatca aaaaccgcga agttccttat
    13501 ctacgcagag aaatcggcgt ggtgttccaa gactataaat tattaccaag aaaaacagta
    13561 tatgaaaatg ttgcgtacgc tatgcaagtt atcggcagaa aacctcggga tatcaaaaaa
    13621 cgagtaatgg aagtgctgga ccttgttggt ttgaaacaca aagttcgagt ctttcctagc
    13681 gaattatccg gtggggaaca gcaacgtgtc tctattgctc gggcaatagt gaatacgcct
    13741 aaagtactta tcgcggatga accgacagga aatctggatc cagaaaactc atgggaaatc
    13801 atgaaattat tagatcggat caatgcccaa ggaactactg tcgtgatggc tacacataac
    13861 agtacgatcg taaatacgat tcggcaccga gtaattgcta tcgaaaacgg ccgcattatt
    13921 cgagatcaag cggaagggga atatggatac gatgattaga acctttttcc gccatttatt
    13981 agaaagtatc aagagtttga aacgtaacgg ctggatgaca gttgcttcag tcagtgcggt
    14041 aacgatcaca ttgactttag tcggtgtatt catggcagtt atcatgaacg caactaaatt
    14101 agctcaagat atagaaggaa acgttgatgt ctctgtattc gtagatatcg gtacaaaaga
    14161 cgaagagatg aaaaagctag aaaaagaatt gaatgactta gatcatgtta aaaaagttga
    14221 attttcaagc aaagaacagg aattgaaaaa aatccaaaac gaaatgggtg attcctggaa
    14281 attgtttgaa ggtgacaata acccgttata tgatgtgtat gtagtaagtg ctactgatcc
    14341 ttcttataca aaacaagtag cagaagaagc agcagatttg aagaatgttt ctcgttctga
    14401 ttacggtgga gcttcctcag atcggatttt ccaaatcgct ggtgcagttc ggacatgggg
    14461 attagttgca gcggcattgc tgctgttcgt agcgatgttt ctcatttcga acacgattcg
    14521 gatcacgatt ttgtctagac agcgtgaaat tcaaatcatg cgtttagtcg gtgcaaaaaa
    14581 tggatatatt cgctggccgt ttttccttga aggtggctgg atcggtctga tcggtgcgat
    14641 tcttccgatt ttgatcatca catttggtta tgcatggttt ttccaattga tcaatccatc
    14701 attattacgt tcccattatt cattgattca tccacaaaat atcgtttgga aaatcaatct
    14761 tttaatggtc ggcattggtg tgatcattgg ttcacttggc tcgatcattt ctatgagacg
    14821 tttcttgaaa ttctaatacc acaatataaa taaaatgaga aagttggaca gtttcgttcc
    14881 agctttcttt ttttattggc taggagaata gaaggaagcg gcttttttcc ctttacttta
    14941 aaaaacaact tgggtattct taagttggta gaagaagagg tgaagccgtg aaaattatcg
    15001 ttgacaattt ttttacgatc cttttgatta tgaatatttt gctttccttt atcatcgttt
    15061 ttcgagaaag aaaggaaact gctcaaacat gggcatgggt gctcgttttg atgttcattc
    15121 cagttgtagg atttatttta tatatttttt taggaagagg gatttccaaa gagaaaatct
    15181 ttgatttgaa gatccaaggg aaaatcggta agaacctgga aattgaagaa gacaaacagg
    15241 cattgatgcg agggctttat cctcatccgc ctactggaca agtagatgtg aaacaattga
    15301 tctatatgct cacggtattt gaaagtacgc tctatacgac taaaaatgaa atcactttat
    15361 ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga tatcaagcag gcagaagacc
    15421 atatccactt ccagtattat atttatcgca gtgatgcatt aggtgaagaa gttcgagatg
    15481 cattgacaga ggcggcaaga cgagggggaa aagtcagagt ccttcttgat gcatggggtt
    15541 ccacacaagt ttcttccagc ttttttcaga atttgaaaaa agcaggcgga gaagtcgcat
    15601 ttttctttcc tctatttgtt ccgtatatta atcctcggat caattatcgc aatcatcgta
    15661 aaatcgtagt tattgatggg aaaatcggat atacaggtgg gttcaatgtt ggaaatgaat
    15721 atctaggatt ggtaaagaaa tttggttatt ggcgagacaa tcatttgcgt atttacggag
    15781 aagcggtgta taccttgcaa aaccgttttt tgatggactg gaattctcag catacaaaag
    15841 aagtaggcta ttctcccaaa ttttttccgt ccattcactc tactggtgat atagctgttc
    15901 aaatcgtgac aagcggacca gataccgaac atgaacaaat caaaatgact tatttgaaaa
    15961 tgatttcgtt agccaaacgg gagatattga tccaaacgcc ttattacata ccggatggat
    16021 cgattcatga agcactgaag ttggctcttc tttcaggcgt aaaagttcat atccaaatac
    16081 ctaacaaacc agatcatctt ctcgtttact gggcaacgta ttctttcgct gcagagctaa
    16141 tcgagtatgg agcaaggatc gaaacatatg agaatggatt tattcatgca aagacgatga
    16201 ttatcgatgg tggcattgtt tcagttggct cagcaaatat cgatgttcgt tcatttcgct
    16261 tggactttga ggtcaataca ctggtttatg atgaacgaat ggcttcacgt gtaagaaaag
    16321 ctttttttga agactcgaaa atttctacgc atctcacaaa agaaatgtat gaaaaccgag
    16381 gaataatcat caagatgaaa gaaggattag caagattgat ttcgccgctc ctttaaaaca
    16441 taaaaaggac atgagaacca tcaacagatg gagatctcgt gtccttttct aacggggcca
    16501 tttctgagag tacgtcagaa atggagaaag cgtctcgcat gagcgaaatt aactttgggg
    16561 tatctcttgt tttttgggga aagcaagaaa taagctaata tatgctatta aaagagacac
    16621 atttcaaagc cagtatagca tagaaacttt taatgagata gtttttttta caattatgag
    16681 aatttatttt gttatttaat gattttgatt cgttttcatc aagtaatgaa aatgataaaa
    16741 taaacatact ttaggattaa aggagatttc taatgaaaaa acttaaatta ttcttaccaa
    16801 taataggatt agctattctt ttatcaggct gttcaaagtg gattgatcga ggagaatcca
    16861 ttacggctgt tggctcttct gctttacagc ctttagtaga aacagttgct gaaacttacc
    16921 agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg cggaagcgga actggtttgt
    16981 ctcaagtaca gtcaggagca gtcgacatcg gaaactctga tttgtttgcg gaagaaaaat
    17041 cggggatcga tgcctcagca ttagtcgatc atcgagtagc agttgttggg atcactccta
    17101 ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga aaacctgaaa aaactattta
    17161 caggcgagat caaaaactgg agtgaagtcg gtggaaaaga tcagccagta gttatattga
    17221 accgcgcttc aggaagcgga acccgtagca cgtttgaaaa atgggtattg gacggagaaa
    17281 cagctgttca ggcacaagaa caagattcaa gcggaatggt gcgccagatc gttgccgaca
    17341 cacccggtgc aatcagctat gttgcttttt cttatgtttc cgatgatgta gatacgttga
    17401 atgttaacgg tgtagaaccg acagatgaaa atgtgacaac gaacaaatgg acgatttggg
    17461 catatgagca tatgtacaca agagatcatc caaaaaaact gacaaaagaa tttttagact
    17521 atatcttatc tgatgaagta caagataaca tcgttggaga actaggttat atccctgttt
    17581 ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt taaagagtaa tctttacaca
    17641 aaatttacaa acttagctaa acttttacat gttattaaaa ccaaattgat gttcaatttg
    17701 tacactgagt gagtatgtta aacctgatta agggggagtc atctgtggaa gatgtgaaaa
    17761 aagtattatt aacaaaatca aaacgttcaa aaatggaaca gcgtggaaaa ttcatcagtt
    17821 ttttatgtat cgcgctgatc gtgttggttg tattatctat tttttatttt gtagctagta
    17881 aaggattggc cacctttttt actgataaga tcaatgtatt tgattttctg tttggtacag
    17941 attggaatcc aagccaaatc ggagcaaatg ggaaaccaat ggtaggggca ttgcctatga
    18001 tcacaggttc gtttattgtg acctttttat ctgctatcgt tgccacgcca tttgccattg
    18061 gtgcagcagt attcatgacg gagatctctc ctaaaaacgg aacgaagatc ttacagccgg
    18121 tcatcgagct gcttgtaggt attccttctg tcgtttatgg ttttatcggt ttatcagtta
    18181 tcgtgccttt tgtcagaagt atttttggcg ggactgggtt tggtatctta gctggtacat
    18241 ttgtgttgtt cgttatgatc ttgccgactg tcacaacgat gactgttgat gcattaaagg
    18301 cagtgccacg gcactatcgg gaagcttcat tagcgcttgg tgccacaaga tggcaaacaa
    18361 tctacaaagt cgtgctacga gcagccgtgc ccgggatttt aacagcagtc gtttttggta
    18421 tggctcgtgc gtttggtgaa gccttagcta tccaaatggt catcggtaat gcagcattga
    18481 tgccaacagg tcttactact cctgcttcta cattgacgtc catcttaaca atgggaatcg
    18541 gaaatacaat catgggaaca gttgaaaata atgtgttatg gtcattagcg ctgatcttgc
    18601 tattgatgtc attattcttt aatatcgtga tccgtatgat tgggaagaaa ggagagctga
    18661 aataatgaac gcaaaacgct cagataaaat tgctacaggt gttttatata tagtttcggg
    18721 gatcatcgta ttgattttag cagctttact actttatatc ttagttcggg gaataccgca
    18781 catttcatgg tccttcttga ccgaaccttc caaagcctac caagaaggcg gtggaatcgg
    18841 gatccagcta tttaactcga tttatttgct tttgatcacg atgatcatca gttttcctat
    18901 ttctttaggt gcgggtatct atctttcaga atatgctgga aaaaactggc taacagatgt
    18961 gattcgcacg tcgattgaaa tcttgagctc gttgccttct gtcgttgtcg gtttattcgg
    19021 tttcttggtt ttcgttattc aattccagta tggtttctcg attatttctg gtgcattggc
    19081 tttgactgta ttcaacttgc ctcttttgac tagaacagtt gaggagtctt tacaagcggt
    19141 ccattacaca caacgagaag caggattggc cttaggtttg tcacgctggg aaacagtgat
    19201 aaaagtggtg gttccagaag cgacaccagg gattttgact ggtgtgatcc taagttctgg
    19261 aagaattttt ggtgaagcag ctgcattgat ctatacggct ggacaaagtg cacctgcgtt
    19321 agattttacg aactggaatc cattaagtgt ttcaagcccg atcagcattt tccgtcaagc
    19381 tgaaacatta gctgtccata tttggaagat caatacagaa ggcacgatgc ctgatggtgt
    19441 ttctgtttcg gctggcgctt ctgccgtgtt gattattgct gttttactgt tcaacttcgg
    19501 cgctcgtgcg attggcaaac gattataccg aaaaatgact tcagcctaag gagaagaagc
    19561 aaaatgaaaa aatataactg gaatgaaaca aatgtcatca ccatgccaaa agagaaagaa
    19621 gtagcactat atacggatga tctacatgtc tggtatggcg acaatgaagc aatcaaagga
    19681 gtggatctgg aatttgaaaa aaataagatc actgcactga ttggtccttc cggctgcgga
    19741 aaatctacgt atcttcgttc gttgaatcga atgaatgatg ggatcgctaa tacgaaaatc
    19801 actgggaaaa tcatgtatca aggcgtagat attaatgcgc ctcaagtgga tgtttatgag
    19861 atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc cgttcagcaa gtcgatttat
    19921 gacaatatta catttgcttt gaaacagcac ggcgaaaaaa acaaaaagaa attagatgaa
    19981 atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc aagtaaaaga cagcttggac
    20041 aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt tatgtattgc tcgtgcgatc
    20101 gcgatgaaac ctgatatctt gctgctagac gagccggcaa gtgctttgga ccctatttcg
    20161 acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag actatacgat catcatcgtg
    20221 acgcataaca tgcaacaagc agctcgtatc agcgactata ctgccttttt ctatttagga
    20281 aaagtaatgg aatatgacaa aactaacaag atatttacca gaccaaaaat tcaggcaacc
    20341 gaagattatg tgtccggtca ttttggttaa gacggaggcc tcaacatgac aaaagagatt
    20401 atttcatcta aagatcttca tctgtattat ggaaagaaag aagcactaaa agggatcgat
    20461 ctaagcttca atcaagggga aatcactgcg atgatcggtc cttctggttg cggaaaatcc
    20521 acttatctgc gttctttgaa ccggatgaat gacctgattc cgaatgtaac gatcaccgga
    20581 agtgttttat ataaagacaa agacatctat ggtccaaaaa cagatgttgt tgaactaaga
    20641 aaagaaatcg gcatggtatt ccagcagcca aacccatttc ctttttctat ctatgaaaat
    20701 gtcatctatg gattgaaatt aaaaggggaa aaagataaaa aagtccttga tcaagtagtt
    20761 gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga aagacaaatt gcataaaagt
    20821 gctttatctt tatccggtgg gcagcagcag cgtgtatgta tcgcacgtgt tttggcagtt
    20881 aatccggaaa tcatcttatt ggatgaacca acaagtgcgc ttgatcctgt ttctactggg
    20941 aaaatcgaaa gtatgctgtt agaattaaga gagcggtata cgatgatcat cgtgacccat
    21001 aatatgtctc aagcttcacg tatttcagat aagacggcat tctttttaga tggtcacttg
    21061 atcgaataca atgatacgaa gaagatattc atgaatccag aaaaacaaga gacagaagat
    21121 tatatttcag gtcgatttgg ataaggagga aacggaatgc tacgtacaca atttgaagaa
    21181 gaattgctga atctccataa tcagttctac gaaatgggga tgatggtgag cagcgccgtg
    21241 cacaagtcag ttcgtgcata catcaaacac gataaaatct tagcccaaga agttatcgaa
    21301 aacgatataa atatcaataa catggaaaca cgcttggaga agaaaagttt tgaaatgatc
    21361 gcattgcaac agccagttac aactgatttg cgtatgatca ttacggtcat gaaagctagc
    21421 tcggatttag aacgtatggc cgatcatgcg gtgtctgttg ctaaatccac gattcgcgta
    21481 aaagggcaga cgagaatacc ggaaatcgaa aaagagatct ccgatatgtc cgattatgtg
    21541 aaaaaaatgg tggacaacgt cctagttgct tatgtcaaaa cagacgaaaa agacgcacgt
    21601 acgatcgcca atatggatca aagagtcaat gactatttca atcgaatcta taatgcaaca
    21661 atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg gaacagacta cttgaatgtt
    21721 gccagttatc tagaacggat cggagactat gtgacaaaca tctgtgaatg gatcgtctac
    21781 ttggctacag ggaaaatcac tgagttaaat acgaaccaca atgagatttt ctaaactgaa
    21841 gctgtgatta tgccgtttgt ttaggaaaag aggttgtgaa atcagcggtt gtgaaaaagc
    21901 tgttctgcat caagtaataa gaacagtcaa acaaaaaata gctcctcata tttttcgttt
    21961 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattcggg aaagggtttg
    22021 tgacaaaaca tgtcacaaac ccttttcatc atttcataga aaggataaag aatgcaaaaa
    22081 aaaattagca tcatcactat attttttatt tgtgtgctta cttcatgggt tattcagcat
    22141 caagccgctt tatatgaagt acctgtcatc gagatagaaa aagtacgaat aaaagaggat
    22201 catcaattga ttacaggaaa actgatgaac acggatcaaa aaggagaatc gatttattta
    22261 tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata taaaagcgag acaaatgtat
    22321 ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa aaaaaaatga tggctggctt
    22381 ttcttcttca caagtatctt cattggcgcc atgttgctta ttggtggaaa acagggtcta
    22441 tttgctttag gaagtctcat catcaatatc gttttacttt tacttttgtt gctcatcttt
    22501 actaaaacag aagggatttc tctattaaat atatctatcc tgttcgtcgt tatagggatg
    22561 attattagca tattggcttt agatggatgg aatagaagca gtataatcaa aatttcagct
    22621 gcagttacga gtgttttttt agcatttttt atttgtcttt tgacaatgaa tcattggaaa
    22681 gaccaaggac tgcgctatga ggaactgaat tatctgacac gaccgtatcg ttctgttttt
    22741 ctttctagcg tactgattgg aacagctgga ggaacactcg ataccgttat cacagtcatt
    22801 gcgacattag aagaattgac taagcgacaa ccagaaataa cagcgaaaca gttatggctt
    22861 tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga ccaatatcct tttgttcgtc
    22921 tatatcagtg ggtcagttcc atcgtttctt ttatatttag gaaatcaatg gtcgttcaaa
    22981 gaaacgatag aacagcattt atcattggaa tttttacgcg tcattgctgg cagcttagga
    23041 atagtattat cgatccctat ctcggtcctc ttttttctga ctgtaaggag gctgaaacga
    23101 tgaatgtact tattatactt gttgggattt tagcaatcag cgtgctactt attggcggta
    23161 cgcaaggtat gtatattctt ttaggcttat tcatcaattt agggatcttt ttcctgttgt
    23221 tattcggtta tcatcagaag tggccgatac tggcattgtc ggtcatagga tttttattga
    23281 ttgctgtggt tatcttattt tttatcaacg gttataatct aaaaatgcgt gcagcttttg
    23341 tgagtatact gatcttttta ttttgtttcc tactattgct tcctatcact gattttttag
    23401 caatccaagg ttttacatcg gtagaactag aagaattatc tggcttagat aagactttag
    23461 cgattgattt tagattactt acgagatcgt tgcttctgat tagtcttagt ggtgctgttt
    23521 tagatgcgag tgtagcaatt tctagtggga cattcgaagt atatcaggca aatccgcaat
    23581 tatcttttta ccaattgcgc cattctagtt tcgctattgc taaaaaaata ttggcgtcaa
    23641 cagtaatgac acttcttttt gcattcatgg gaagttcttt agcgttgatc ctctggttta
    23701 ttgatttaga cattcctttt cagcaagtga tcaatgaaaa atcatttgtt ctagaatata
    23761 cgatggctat tcttaccaca ttgtccgcac tacttgttct tcctttaacc tgcgtgacta
    23821 cagcatatct ctttacaaaa aagaaagaac aactcaagat ggagtaaaag ttacaactta
    23881 agtcctatga catttttact gaatcagtgc ataatatgga tgtaaggaaa agcttgcaaa
    23941 caattttgct taaggaggca atttccatga aagaaagaga acgtatttta gatttagtga
    24001 aaaaaggaat cctttcgact gaagaaggct tagatttatt agaaagcatg gcaactgaaa
    24061 aagacgaaaa gcaaatcaaa aaagaagcag acaaggtaac tgctagtcat aaagaaaaag
    24121 atcaggcaag tcaattgatc gataaacttg aaaacggcga agaagaaatt tctgagccag
    24181 ttgatccaaa ggaaagagaa agacaagatc aagaaaactt agaaaagatc ttagatgaac
    24241 tagcaactga agcgaataaa acttcggcgc gtctagatga agtaaatgct caattcgctg
    24301 acaataaggc agcacgcaat gagaaacagg aagcattgat gcagctgaat acaaaagaag
    24361 aattaggcga gttgacagaa gaagaattag cacagcgtca gacgttagaa gcagaaatca
    24421 aagaattaga agctagtggc gatacactgt tagaagaaca attgaaatta gaagcagaat
    24481 taaaagatat ccgtaagaat caatggtctg agaaaaaaga aacgtttact gataagtttg
    24541 aattgccaga tgattggaaa gatcaagcaa cagatacact gaatcaagta ggcgaaaaaa
    24601 tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa aacattccaa actgtttcag
    24661 aaacagtgaa tgacaacatg gaatggaaag atgtcagctt acgtgtccct gggattgcga
    24721 caacgaaatt tgagcatgaa ttttactatg aagctcctgc agcaagtatc cttgatatca
    24781 aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg aaagttgaag
    24841 ctaagatcaa actttacgga aaaatgggtg ctgaaccatt tgaagcattc tctgaacgga
    24901 gccagattga ggttaatgaa gatcatatct ccttccaaat tccaaataaa cgtgtgcgtg
    24961 cagaccttgt tttctactta cctaaacgag tatacgacca cgcagctatt aaacttctaa
    25021 atggcaacat catgattgaa acattggaag ctaaagatat ttatacaaaa tctacgaatg
    25081 gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt gtaaacggca
    25141 atatcgatat tcgaaacggg aacatcttag attctattat cgaaacagta aatggtacag
    25201 tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt ggtaaatggt gatgtacgtc
    25261 ttacgattaa agaagataat ttgaaaaaag tagaagcaag ttcagtgaac ggaaatgtca
    25321 aagtcgcttt accagatggc atcggtttgg aaggacatgc taaaacaagt cttggaagta
    25381 tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg acaaaccaaa
    25441 tgttgcaatt ccgtcgtgtt tcagatggtg aaatcgcgca agtgcaactt tcaacgacaa
    25501 caggaagtat ctatttaaaa gatacagata aataaagagg ttgtgaaaga gatgttcagc
    25561 cttgagcaat aagaagcgat tttgtaaaat agcttcccct attttacaaa attgtgcctt
    25621 attgccgata gtctatctct tgaacaccgt ttaccaagag attatgaacc tgccaattat
    25681 cgccgagtca caaggaataa tttcagaaaa agctcgtcac gttttatgaa tatggtaatt
    25741 tgtgaccgag aattttgcag aaagacaccg attaagaaga aggttgtcac aacgattttc
    25801 ttcatttaaa atagtagaac agaggtgaga catgatgaaa aaaaagttga caaaatctaa
    25861 taaaaatgtt gttttgacag ggactttagc aggaatagga gaatacatcg ggattgatcc
    25921 gactgttatc cgtgtgcttt atgtattcct gagcttagtt gcttttggaa gtccaattat
    25981 tttatacatt ttattagctt tgattatccc atctgcgcgt tctgcacgaa caaactatgg
    26041 acataacaat gcatattatg aaaaaaacaa ttatcgtgaa caaactcgtg aacaaacaaa
    26101 aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg agtgactttt aatgtcgtac
    26161 tttcaacgta ttttagttaa tacgttaaca ttcatatcat tggctgttct ttttcctgcg
    26221 atggtccatg tgagcagtat ttggacggcc gttcttgcca gcttcgtatt gtctgtgttg
    26281 aatatgtttg tcaaaccgat tttgacgata ctgtccctgc cctttacatt agtcacattg
    26341 ggattgttta cgtttgttat caatgcagcg attcttaaaa tgacctcctt ttttgtggga
    26401 gcagaacatt ttagtttttc ttcattcggt gcagcgttgt tagtagctat cttgatgtcg
    26461 cttgtcaatg taattgtcag cgaacataac agtagaaaat aaaagaaagc tgtgccgaac
    26521 tatcggcgca gtttttttat ttagaatcag gaatgcgttt tctattacat tggtaattaa
    26581 ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa atgatagaga
    26641 acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta gccaattagt
    26701 gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa agcttagacc gtgtcatcgt
    26761 aactggggac atctcacgcc caggactaga gttgactggt tattttaatt attattccca
    26821 taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga tgatcccaga
    26881 agagcgtttg atcatcatga gaagattatg tagtgaagat atgcccgcat ttattatttc
    26941 aagaggatta ccagctccag aagaaatgat ccaggcagca aatgaaaatg ggatagctgt
    27001 actgcgttct cccatctcaa cttctcgttt attaggagaa ttatccagtt atttagatgg
    27061 tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg gatgtttacg gattaggtgt
    27121 actgattcaa ggtgacagtg ggataggaaa aagtgaaaca gcgttggagt tgatcaaacg
    27181 cggccatcga ttgatcgcag acgatcgagt agatgtttat cagcaagatg aattaacggt
    27241 gatcggagaa ccgcctaaaa tcctggaaca tctgattgaa atccgaggaa tcgggatcat
    27301 tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc tctatgcaag tccagttggc
    27361 tgtttatctg gaagcatggg caaaagataa gaaatacgac cgtctgggct ctgaagacac
    27421 gaccgttgaa atcgcggaag tgggcattcc acaaataaaa atccctgtca aaacaggacg
    27481 aaacgttgca atcatcattg aagtagccgc gatgaatttc cgtgcacgta caatgggctt
    27541 tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa actcaggcaa
    27601 cgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt tgagcttgta
    27661 gggattccta tctactggta tgcattgatc atcgtatcag gtatcattat cgctatgtgg
    27721 cttagcagca gagaggctgt tcgggtagga ttaaaagcag atgatgtgac agattttatg
    27781 ttgtggggat tgcctatctc cattattggc gctcgcttat attatgtttt atttgatctt
    27841 ccccagtata tagctgatcc aatccaaata ttcaatatcc gttcaggagg cttagctatt
    27901 tatggcggac tgattgcagg cgcgatcacg ctgtattttt ttactaaata tcattttatt
    27961 tctttgtgga cttttttaga tatcgctgca ccaagtgtat tattagcaca ggcaatcggt
    28021 cgttggggaa atttcatgaa ccacgaagca tatggaccag acacgacgcg aagcttctta
    28081 gaaagtctgc atttaccgca attcatcatt gataatatgt atatcgaagg gatctatcgt
    28141 caaccaacat ttctttatga atcactttgg agtttgattg gatttatttt gcttttgttt
    28201 ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc ttttatgtta tctaatgtgg
    28261 tatagtttcg ggcgtttctt tattgaagga ttacgtacag acagtcttta ctttttaggt
    28321 gtgatccgta tttctcaatt attatctgct gtgttatttg tcggtacatt gattctattt
    28381 atttggcgca gaaaaaaagg aaatttaccg gattatgacc gctcattagg aaaaaatcaa
    28441 caaattatct gatttttaga gcgttaaaaa tgaaaaaaat ttcaggagtg gtaaaatgaa
    28501 ttatagagta gcggttttag gaccaggctc ttggggtact gctttggcac aagtcctcgc
    28561 cgaaaacgga catgacgttc ggatttgggg acatcatgct tcacaagtag aagaaatcaa
    28621 cacccaacac acgaatcaac gttatttacc agatcattat ctacccgatt ccatccaagc
    28681 attcaccaat atggaagatg cagtaaaaga cgcagatgcg gtgctattcg tcattccaac
    28741 gaaagcaata cgttctgttg caaaagaact agtgcctaaa ttgaatacaa agccagtgat
    28801 catccatgca agtaaaggat tagaacaggg aacacataaa aggatctctg acatcttgtt
    28861 agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt ctttctggac caagccatgc
    28921 agaagaagta gcaaaacatg acatcactac gatcacagct gcaagtacag atgcaaaagc
    28981 agcaaaatat gtacaagagc tgttcatgaa taattatttt cggatctata ccaatcctga
    29041 tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt attgctattg gtgctggcgc
    29101 tattcacggc ttagggtttg gcgataatgc aaaggcagca atcatgacgc gcggattagc
    29161 tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta tcggattaag
    29221 cggaataggc gatctagtcg ttacttgcac aagtgttcat tctaggaact ggcgcgcagg
    29281 caacttgtta ggacaagggc agcccctaga agaggtgcta gccaatatgg gaatggtcgt
    29341 tgaaggtgtg aatactaccc aagcagcgat ggaactagcc caaatgatgg aaatcgaaat
    29401 gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca aaacagccgc
    29461 aaaagaaatc atgttacgtg atggaaaaaa agaaaatgaa ttctcattaa caattttttg
    29521 aaggagcgaa tgtatatgaa agttaaaaaa gcagttattc cagcagcagg attaggaaca
    29581 agatttttac cagcaacaaa agctatggca aaggaaatgt tgccaattgt tgataaacca
    29641 acaatccagt tcatcgttga agaagcgttg aaatcaggta tcgaagacat cttgatcgtt
    29701 acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatattga attagaaaca
    29761 aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag tagaagaaac gacagatgtc
    29821 aacttgcatt tcatccgtca atctcatcca aagggtttag gacatgccgt tcttcaagca
    29881 agagccttta ttggaaatga accatttgtc gttatgctag gcgatgatat aatggaagac
    29941 gaagtaccat tgacacagca attgatcgat gactatgagc gtacacacgc ttctacgatt
    30001 gccgttatgc aagttcctca cgatgaaact tcaaaatacg gaatcatcaa cccaggggaa
    30061 gtactggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga tccaagcaaa
    30121 gcaccaagtg atttagctat tattgggcgt tacttattga ctccagaaat ctttgatgta
    30181 ttatcagaac aacaaccagg tgctggaaat gagatccaac taactgacgc aatcgatacg
    30241 ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta tgacgttggt
    30301 gacaaatttg gttttatgaa aacaagtatt gaatatggtc tgacacatcc agaagtaggg
    30361 gcacctttgc gtgaatacat catgtcttta ggtaaaacat tagaagcaga aggaagcaag
    30421 atcaatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa ataatctttt
    30481 tttctaacga gcttcagcgg ctaattgaat aaaaagtagt tgaagctcgt ttttatttgt
    30541 ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga gtttgatcat
    30601 gtcaggagga gaaattgcag gattgatcgc agcaatcgcc tttgcgatac ttgtcgtttt
    30661 catcgttcgc gtattgttgc aagtttccaa aactttagaa aaagtagacc agactgtcgc
    30721 agaagcaaat acgacaattg agatcgtcac acaagacgta gatcttttat ctagacaagt
    30781 agaaggcttg ctagtcaaaa gtaatgagct gctcttggat atcaatggaa aagtcgcaac
    30841 gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcgg aacttaatca
    30901 ttctagtaaa aacatcgtga cgaaagtcgg cggaatcgga aaaacgacag ctaaagcgac
    30961 tgttgccgga aaagtcggtg gaacagcgat gaaatttttt aaaaacaaaa aacatactga
    31021 aacgacagga ggaaaataag aatggctaaa aaaggtggat ttttattagg tgcagtagtt
    31081 ggaggtacag ccgcagcaat cgccgcatta ttgttagcac ccaaatctgg taaagaactt
    31141 cgggatgaat tggctgctca agcagatgat ttgatggaca tggcttcaga ttacacagat
    31201 atggcaaaag aaaaatcatc tgagttaaca ggtattgcaa aagaaaaagc gcaaggtctt
    31261 tctcaacaag caggggatct agcaggcaat gttaagcaga aggtaagtga atcagcagag
    31321 gaagcaggaa gtgaatcaga tgatatcttg ttaaacctca aagaacaatc agctgattta
    31381 tctaatcgtt tcaaaaaaac agcagaagat ctgaaaaagc cagcagacga attaggtagt
    31441 attgctaaag atacttcaga agatatcatg atcgatgtaa aagatgctgc agataaagca
    31501 ggagagacgg tcaatgaagg aatgtcagaa gcaaaaccag ttgcagatga cacaaaagaa
    31561 atgttgaaaa tggcaaaaga agatgcaaaa gaagctaaaa aagaaatgaa agatacaatg
    31621 acagattcga ctaccaatca ctaagattac tcatttctag aataaaaaag gtgagagcgg
    31681 accagctctc acctttttta ttttcattac tctaaaacaa gtaattcttt tggtgtagtc
    31741 gtgaatggca cacaaccatc acttgtcaca tacacacaat cttcgatacg tacccctact
    31801 ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc aatcactaag
    31861 tcgtttcctt ctacaagaga agggaattcg tggacggttg ttccgattcc gtgacctaaa
    31921 cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc gatttgatca
    31981 agttcacctg ctgtcacacc tgggcgaact gcctcttgtg cggcaagctg tgcttcaaga
    32041 gtgatgttat agattttttc ttggaactca gttggttttt gataagcaac tgtacgagta
    32101 gcatcactgc aatagccatt ccaaataacg ccaagatcga ataacactag ttcatttggt
    32161 gaaaccttcg tgcttcctgg tgtaccatga gggcttgcag catttgctcc tgcaaggact
    32221 aatgtgtcga aagacatttg tgaaacacct tgttttttta attggtattc gatttctgcg
    32281 ataattgctt gttcagttac gccttctttt actgttttga agccaatttc aaaggcaaca
    32341 tcggcccatt taccagcttc gatcagtttg tctttttctt ctaccgtttt gatcagttgc
    32401 agacgctgga taatagaagt tagatcggtg taagaatctg tacctgtcaa gatatcagag
    32461 atagcatcga agcgatcgac agtcagagca ttcttttcaa tacctacatt accaggttgc
    32521 ccatagcgtg cgcgaagctc ttttccgatg atctcccaag gattttcgct gtctaagtag
    32581 ccacgtacat cgtacgccca gctgctgttt tgagcatctt caacttccag agcaggagtg
    32641 aaaaggaaag gatcttgttc cagagggata aaaagagcta gtacacgctc gtgaggatca
    32701 ctttcatatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc caattctact
    32761 tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat ttggcaacct
    32821 actttctaaa ttaattttgt attcaataat tgtaccactg attgattaaa agaccaacgt
    32881 tcttaaaact gaaaaaagta aaaattttca tgaaaacaga gataaaacgg ttgcaattgt
    32941 tgagattatt tgatatccta ttttagaaat gtaagacgta ttttcagaaa acatataaaa
    33001 tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg tagcgagaga
    33061 ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc
    33121 agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc caaatgcagt
    33181 cgcacgtggc ttggcaagta agaaaacaac aacagttggg gtaatcattc ctgatgtcag
    33241 caatatgttc tttgcctctt tagcacgtgg tatcgacgat gtggcaacaa tgtacaaata
    33301 caatatcatc ttagcaaact cagatggcaa cgatcaaaaa gaagtcaatg tattgaataa
    33361 tttattggct aagcaagtgg acggcgtgat ctttatgggt caccatatca cagacgaaat
    33421 ccgtggagaa ttttcacgtt caaaaacgcc tgttgtacta gctggttcca ttgatccaga
    33481 tgaacaagta ggtagcgtaa acattgatta tgtgtcagca acaaaagatg cagtaaataa
    33541 attagcaaaa agcggcaata aaaaaatcgc ttttgtcagc ggtgcgctga ttgatcctat
    33601 caatggtcaa aaccgtctaa aaggctataa agaagcatta agcgaaaaca atctaccata
    33661 tagcgaagga ttgatttttg aggcacaata caactttaaa gatggtctgt ctctagttga
    33721 ccggatccat aatagcggtg caacagctgt ctatgtttca gacgatgaat tagccattgg
    33781 tatcttggat ggtttattag accgcggcgt aaaagtacca gaagaattcg aaatcatcac
    33841 aagcaacaat tctttactga cagaagtagc tcgtcctcgt ttgacaagta tcacacaacc
    33901 attatacgat ttaggagcgg tatccatgcg cctattgacg aaaatgatga ataaagaaga
    33961 agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat caactaaata
    34021 ataaaagatc aatcatttaa aggttgttcc aaaagtggtc aacttctgga acaacgttta
    34081 tacgagaaag ggggttatga caaaattttg tcataacccc tttttttttg tgactttaaa
    34141 aaaagagaaa atggcggaaa caaaccttac gtccatcgta tacgacacaa tctgtgcgta
    34201 gatgctacat tttgttcact actatcaaaa ttactttaaa atgaaaacgt gataaaaaag
    34261 aaagtagaaa agtagataag taactctaaa aaatccaaaa aaatccaggc tcgattgcgt
    34321 acttatctta aaggagaaac aatccatcat atataacccg aaaggagact tctatttgcc
    34381 taagaaaaga cgaaagacca tagtcatcat tttgaccatt cttactatag gaatcggagg
    34441 gaaattttac atggacaaaa gagaaatgca aagacagaaa gatttactcg cagtagaaaa
    34501 acagtccgtc aaagttctga aaaatacctt tgcggatatc aaggaagtga agattgaaaa
    34561 atctgctcga aatgaaatga cgggttcata ccggatagtt attctaatga ctaataaaca
    34621 agatcaatcg atttattttt cttatagttt ttggaaagaa agaaatgaga taggaagtta
    34681 cggaatagtt gatgaaaaaa agcaaaaaga aggaaatacg ctcaataaag ttaaagtaac
    34741 ttactctaat ggaaatgagg aaagtattta atgattactg acaaacaata tagtgaatta
    34801 agtgatgctg tttactggct agatcctaaa catagagact atgtacctga aatgcaagaa
    34861 aatttaagtt ttaaaattaa cggcactatg tataaaatcc taaaaatcaa aaactccttc
    34921 gacggcatgc aggcgatggc tgtagcgcca atcgtccata gcaaattgga gaaaaatttc
    34981 aaaaataaaa aaattcctgc aaactttcgc gtacttaaat aatgaaggtg tcaatactta
    35041 cataaaaaga aaggaggcac gttcttgtca aggaaaataa gaaaactcag cttagccatt
    35101 ttattaatcg tcatattagg gatcggaggg aaagtctata tggacaaaag agaagcacaa
    35161 aggcagaaag atttactcgc agtagaaaaa cagtcagtca aagttctgaa aaataccttt
    35221 gcggatatca gggaagtgaa ggtagaagaa tttaaaaaaa accctgttac cggaagttat
    35281 ggtgcacttg tgacaatgac aaataatgaa gggaaatctg tttattttga ttatagtttt
    35341 tggaaggaga gaaatgaact tggtggtttt ggcattgaga acagagcggt acaaaagaca
    35401 ggagtcacta taaaaagagt aaaagtaatt ttttctaacg gacaggagga gttagtatga
    35461 caataaacga taagcattac aatgatatta gtgagaaggt ttattggcta gatcccaaat
    35521 atccccgcta caatgaggga tacaaaaaga actctgttaa agaatttgct ggtatggaat
    35581 ttcaaatcct acaaatcaaa gactcattgg acggcatgca ggcgatgtct gtagcgccaa
    35641 tcaagaacgg taaagtggat ctttctcaag tagtgattgc ttttgcagga acgaacttaa
    35701 gtgattggaa agatttggaa acggatgcaa gaagtatcat gaatctctac gggaatagac
    35761 caggagcaag tttttctgaa aaagcaacaa caaacaagat tttatccgtt agtggattga
    35821 aaacagcggg gcaaatcaat tcagcgattg catttgctaa tgaagtaaaa aaggaatatg
    35881 gagaagctgc tatttctact accggccatt cattaggtgg attcttagca ttatatgtag
    35941 cagcagagaa acaatggaaa aatgtcggct tcaacgggcc ggacccgtat gatctcttat
    36001 caccagaagc aaaaaaatgg gtggaagaaa atcccggtat gctcaccaat taccgaaatc
    36061 acgccgattt tatcggtaat tttggtggta atggcacagg tgctgaagtt cggatcagta
    36121 tggaaacagc attcttgaat ctgcttgctt cccacagttt aacctcttgg aagtttgatg
    36181 aaaatggaaa gctgattatt cctgaaaatg aaaacaatgt taaagctcga aaacaacaga
    36241 aagaaaacca agcgacccag ttgttttcct atcagttgca agaactgaac aacatacgta
    36301 aaaaactacg attaagtggc ggccagctga cagttaatga acaaatttac ttagatgaca
    36361 cacaagctag gttagtagtg gaaaagttat cggcaaaaat gaaaagcgct atggaagaag
    36421 ttatccgcat caatcaaaga ggaatttttg agctagagaa aaaatggcaa gaaggcttgc
    36481 ggcaagcacg tgttgtagcc ccttcgttga ctgaacagga aatcatagaa gcattagcta
    36541 gtgtgggcgc cacaaaagaa aacttagtcg attctgcgaa agaagtctat ctgctacgaa
    36601 tcaataaggc aagacgtatg ggggaggcct ttgatacatt ggcgaaggag atccaagaca
    36661 aaatcaatga aatcgtggca cgagatcgag aattagcaca gcaattcaat taaaggagaa
    36721 gtagatgaaa ctagaagaat tggaaagaga ttatcgaagt gaacgaagac aattagaagc
    36781 tcaagaagat gaattgatcc actttaagaa tcaaggatta cgattgatgg agcaagagga
    36841 gcagcgactg catcatgtat taaagaactt agctttagat aatgagcctc tgtttcaagc
    36901 aagacgaagt tattcgaatt atcaggagca ttatcaacaa gccctacaag tagaacgacg
    36961 aaaactctat cagcagcaag atgaactaga acgttcctat cgtcaaaaaa tcagacaaat
    37021 aactatggag aaatcatctt atgaatgaat ttttaatgca attactaaaa gaattagaga
    37081 aaaatctaca tcgatcaaat gtacgttcag ttggtatggg gatatctcag caaatttggg
    37141 cagaaaaacg attgacagat caaaactacc aaatgactgc agaagaagct gctcaagaat
    37201 tttctaaccg acatgtacac ttgaaaggat cagtaaaagg gcaatttgca gaaaaactaa
    37261 ctgaattatt ccaaagacaa gaaaagaaat taaaacagtt tcgttagtaa agttatatag
    37321 aaaaattttg tgaaaat
//
LOCUS       DACELF010000016        37293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.016, whole genome shotgun sequence.
ACCESSION   DACELF010000016 DACELF010000000
VERSION     DACELF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.016"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            112..975
                     /locus_tag="IW687_000803"
     CDS             112..975
                     /locus_tag="IW687_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0950085.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1104..2726)
                     /locus_tag="IW687_000804"
     CDS             complement(1104..2726)
                     /locus_tag="IW687_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0950086.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2975..3496
                     /locus_tag="IW687_000805"
     CDS             2975..3496
                     /locus_tag="IW687_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950087.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3682..5412)
                     /gene="spxB"
                     /locus_tag="IW687_000806"
     CDS             complement(3682..5412)
                     /gene="spxB"
                     /locus_tag="IW687_000806"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0950088.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5577..6395
                     /locus_tag="IW687_000807"
     CDS             5577..6395
                     /locus_tag="IW687_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0950089.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6498..6695)
                     /locus_tag="IW687_000808"
     CDS             complement(6498..6695)
                     /locus_tag="IW687_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950090.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6864..7442
                     /locus_tag="IW687_000809"
     CDS             6864..7442
                     /locus_tag="IW687_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0950091.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8487..8990)
                     /locus_tag="IW687_000810"
     CDS             complement(8487..8990)
                     /locus_tag="IW687_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0950092.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9348..10373
                     /locus_tag="IW687_000811"
     CDS             9348..10373
                     /locus_tag="IW687_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0950093.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10696..11574
                     /locus_tag="IW687_000812"
     CDS             10696..11574
                     /locus_tag="IW687_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950094.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12050..12166
                     /locus_tag="IW687_000813"
     CDS             12050..12166
                     /locus_tag="IW687_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0950095.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13094..13432
                     /locus_tag="IW687_000814"
     CDS             13094..13432
                     /locus_tag="IW687_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950096.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13526..13756
                     /locus_tag="IW687_000815"
     CDS             13526..13756
                     /locus_tag="IW687_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950097.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14156..14527
                     /locus_tag="IW687_000816"
     CDS             14156..14527
                     /locus_tag="IW687_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0950098.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14801..15493)
                     /locus_tag="IW687_000817"
     CDS             complement(14801..15493)
                     /locus_tag="IW687_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0950099.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15496..16281)
                     /locus_tag="IW687_000818"
     CDS             complement(15496..16281)
                     /locus_tag="IW687_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0950100.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16518..17918
                     /gene="lacG"
                     /locus_tag="IW687_000819"
     CDS             16518..17918
                     /gene="lacG"
                     /locus_tag="IW687_000819"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0950101.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17943..18260
                     /locus_tag="IW687_000820"
     CDS             17943..18260
                     /locus_tag="IW687_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0950102.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18273..19946
                     /locus_tag="IW687_000821"
     CDS             18273..19946
                     /locus_tag="IW687_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0950103.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19960..21591
                     /locus_tag="IW687_000822"
     CDS             19960..21591
                     /locus_tag="IW687_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0950104.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22082..23647)
                     /gene="guaA"
                     /locus_tag="IW687_000823"
     CDS             complement(22082..23647)
                     /gene="guaA"
                     /locus_tag="IW687_000823"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0950105.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23706..24629)
                     /locus_tag="IW687_000824"
     CDS             complement(23706..24629)
                     /locus_tag="IW687_000824"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0950106.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24825..25430
                     /locus_tag="IW687_000825"
     CDS             24825..25430
                     /locus_tag="IW687_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0950107.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25738..26754
                     /locus_tag="IW687_000826"
     CDS             25738..26754
                     /locus_tag="IW687_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0950108.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26895..28196
                     /locus_tag="IW687_000827"
     CDS             26895..28196
                     /locus_tag="IW687_000827"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0950109.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28242..28904
                     /gene="deoC"
                     /locus_tag="IW687_000828"
     CDS             28242..28904
                     /gene="deoC"
                     /locus_tag="IW687_000828"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0950110.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28925..29323
                     /locus_tag="IW687_000829"
     CDS             28925..29323
                     /locus_tag="IW687_000829"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0950111.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29389..30471
                     /locus_tag="IW687_000830"
     CDS             29389..30471
                     /locus_tag="IW687_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0950112.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30683..32245
                     /locus_tag="IW687_000831"
     CDS             30683..32245
                     /locus_tag="IW687_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950113.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32238..33371
                     /locus_tag="IW687_000832"
     CDS             32238..33371
                     /locus_tag="IW687_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950114.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33371..34327
                     /locus_tag="IW687_000833"
     CDS             33371..34327
                     /locus_tag="IW687_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950115.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34426..35592
                     /gene="deoB"
                     /locus_tag="IW687_000834"
     CDS             34426..35592
                     /gene="deoB"
                     /locus_tag="IW687_000834"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0950116.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35616..36434
                     /locus_tag="IW687_000835"
     CDS             35616..36434
                     /locus_tag="IW687_000835"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0950117.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36451..37158
                     /gene="deoD"
                     /locus_tag="IW687_000836"
     CDS             36451..37158
                     /gene="deoD"
                     /locus_tag="IW687_000836"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0950118.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11629 a         6510 c         7892 g        11262 t
ORIGIN      
        1 tcttattttg attagaactt gcgaaaataa accggttaaa gtgtgtctag cgcaagagat
       61 ttgctataat agacgataag ggtaaaatgt aacgagaagg ggttgaaaaa tgtgtatcaa
      121 attgtaacag attcttgttg tgatctgcca taccatgtat tagcaaaaga aaaagttgat
      181 tttttatcaa tgaatctcca attgaatgat caagagttag tagatgacct cggaaagaca
      241 ttcaattatg accgctacat gaatcagctg aaaaatggtg cgatgcctac gacatcacaa
      301 atcaatgttg gccgatattt agaatttttc cgttcttatg cggaaaaaca gattcctgtt
      361 ttatatgtgg ctttttccag tggattaagc ggctcttaca gtagtgcgct tcaagcggtt
      421 gaattgctga aggaagaata tgaagaccct gagatctatg tttttgatac aaaagctgca
      481 agtattgggg aagggctttt agtattggaa gctgtaagat tgaaaaaaga aggtaaaagc
      541 atcgaagaaa ccctagagtg gctggaaagt aattatttga aagtccattc atgggtaaca
      601 gtagacgatc tgaaatatct agaacgaggc ggtaggattt ccaaagcaag tgctactctt
      661 ggcagtctat tgagtgtaaa gcctattatt acggtggata aagaaggaag gctgacgaat
      721 gttgacaaag ttcgaggaag aaacaagtcg atccagacaa tcgtcaatga gacggtaaaa
      781 acaatcgttg caccacttga tcaaatcgtt tatgtggctt acgctgggga tctagagagc
      841 gcggagaagg caaaacggct tttggaagag cagataaaga tgaaagatgt gaagctttat
      901 cccttaggac caacgatcgc cagtcacaca ggctatggct gtatcgcgat tttttcgatg
      961 ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa gcccagaaat tgattgtaac
     1021 aaaaacagcg actcttaaaa gatagatgag catcctatct cttagaggtc gctgtttttt
     1081 tattttttaa gctaaatagg ccattatgcc cataactcgt tcaattgttt ttgaatatct
     1141 gcattttcca aaaattcatc atatgtcgta tcagctcgat cgatcacacc tttatccgaa
     1201 acggcaatga tgcggtttgc caatgtttgg ataaattgat ggtcatgtga cgcaaataag
     1261 attgagccag taaatgccat caagccgtca ttcaatgctg tgattgattc taagtctaaa
     1321 tggttcgttg gatcatctaa tacaagtacg ttagctttcg aaagcatcat ttttgaaagc
     1381 atgcaacgga ctttctctcc accagataaa acattcactg gtttcaatac ttcttcacca
     1441 gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt tatcatcttc ttctttactt
     1501 gcatattgac gcaaccagtc taaaatcgtt agatcagtat cgaattcttt cgttgtatcc
     1561 tttggtaaat aagaattact tgttgtcaca ccccaacgta ctgttccagt atctggcgta
     1621 atttcaccca tgataacttt gaatagcgtt gttgttgtta tatcattttc tgcgacgaaa
     1681 gcgaccttgt cttctttgct caaattaaaa gatatatcat ctaaaatttt ctttccatca
     1741 atcgttacac ttacattttc tacctgcaat aagtcgtttc caatctcacg ttcaggtttg
     1801 aatccaacga atggataacg tctagaagaa ggttggatat cgtctaaagt aattttttca
     1861 agcatttttt tacgtgaagt tgcttgtttt gattttgatg cattcgcact aaagcgggca
     1921 ataaattctt ggagttcttt gatttgttct tcttttttgg catttgcgtt tgcttgaagt
     1981 ttagccgcta gttgactaga ttccaaccaa aaatcataat ttcctacata aagtttgatt
     2041 ttaccaaaat ctagatccgc catatgtgta catactttgt tcaaaaagtg acggtcatgg
     2101 gatacaacga tcacagtatt ttcaaaattg atcaaaaatt cttccagcca attgatcgat
     2161 tggatatcca gaccgtttgt tggttcgtct agcaaaagaa catcaggttt accaaatagt
     2221 gtttgtgcta ataacacttt taccttttgt ccaccagtta attcactcat ttgctgatgg
     2281 tgcaattctt cagggatgtt cagaccttgc agtaaaacag ctgcttctgg ttcagcttcc
     2341 catccattaa gctcagcaaa ttctccttct aattcagctg ctcgaatacc atcttcgtca
     2401 gtgaaatcct ctttcatgta aatcgcatct ttttctttca gcacttcata cagacgcttg
     2461 tgtcccatga tgactgtttc aagtacagta tagtcttcat agtcgtaatg gttctgtttc
     2521 aatacggcca tccgttcatc tggtcccata gtaacggaac ctgtggaagg ctgcagatcc
     2581 cctgataaca ctttaaggaa cgttgattta cctgcaccat ttgcaccaat caagccatag
     2641 cagtttccgg gggtaaattt aatattcaca tcatcaaata gtttgcggtc tgaaaagtgc
     2701 aaactcacat catttacagt aatcaatttt tttcctcact tttatcatta ttagttttga
     2761 caagtcttat agaattatat aggggaaagg taaaagattc aaggcaatcg aacattcttt
     2821 gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa gcggagcgaa aatagctact
     2881 ataggattta ttagtttgca acagcataat ttttgacgtt atttataaga gggagtacta
     2941 taaaactgta aaagaaatga aagaaggatt gaaaatggat gaaaaaagaa ccatcgactg
     3001 gggctcattg attttgggaa ttttatttgt acttgtctca ttgttatcat ttcaagaccc
     3061 tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt tttgccttta ttaaagggat
     3121 atttgaactg tttgtacgga acagattaaa agagctaaca ggatataaag gaaagatgcc
     3181 cttaataatc gggatcatcg atattctagt tggcgtcttt ttcttattca atattggtgc
     3241 aggagtcgca gcattgcctt ttgtttttgc agtatggttt attgctgatt cagttttagc
     3301 gttatttact gctgacttgg caagaggagt aagtgaagga tattattggt ttacgatcat
     3361 cgtcaatatt ttaggaattt tattaggaat tttcttattg tttaatccaa tttcttctgc
     3421 gttgacatta agctttttag ttggattcta ttttatgtta tttggtatca cacatatcgt
     3481 ctatgcattt agataagtaa aatcaaacaa ataaaaaagc cggcttccga ttacagtttt
     3541 actgttgtaa ttggaagccg gcttttttat catggacaaa tatgcacgag gtgattttta
     3601 ggtgtcgaga caaatttatg caaaaatgtg agaagctata tacacaaatt ttatcttaat
     3661 actcgaagca tagcgcagtt gtcacaacct cttttcatat aattcattca atactggcaa
     3721 atcatggact tcatattttt ctttgaatgc ttggatttct tcttcagaat attttcttgg
     3781 atctaaatgc agatcttcta ctggcaatgg gcgcttgtta ttgatcttca catcgattac
     3841 tacaggacga tctgattttg cagctgcatc aaaggcaaat cgaagttgcg agtattctgt
     3901 gactgtaaag ctatttgccc cgagcgcttc tccaacttta ccgaaatctg catcttctaa
     3961 aaatacacca aatttctttt gttccgtatc ttcttgttct gcttcgatga aaccaaaaga
     4021 atcattcgaa aagacaacat taataattgg aagctgatat ttgacttgtg taatgatatc
     4081 ttgcatgacc atagcaaacc cgccatcacc acttaaggta aatacttgtt tttctgggta
     4141 gcttagttgt gcggcaattc ctcctggcac accattaccc atagttgcga accatccaga
     4201 tgtcgtatgt ttttgtttac cgttcatatt caataaacga atagaatgga tagtcgtgtt
     4261 accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata cgattgattt ctttatagac
     4321 agcctctgga cgaatcggtt cttcctctcg atcttcaaaa gatttcaacc aatttaccca
     4381 tttttcttta ttttcttgat ttgctaggta ccagctatca gcagggcgag catcccctaa
     4441 ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg atcgacaaag atgtttcatg
     4501 gcgtcgtcca aattttgacg catcgatatc gatttggata aattcagcag ttggattaaa
     4561 aaatgcacgt ccaaatggga agtcgctacc gacaaataag atcagatcgg cttctgccaa
     4621 tgcttcattt ccgggtttag tagcgacacg tccagcaaaa ccaagaaagt tttcataatc
     4681 atcaggaata attccttttg ctagaacgga agcgacaaca ggaactgaaa aatgttctga
     4741 gaattctttg atttgcggaa atccgtttcg cgttccttga ccaacataaa gaactggttt
     4801 ttttgctttt tcaagataag gcaaagcagc tagcaaatct ttttcttctg gtaatatcac
     4861 acttgttttg tgagtgtgag cggtggagaa aggttgttcg tcaatttctt caaaaccaag
     4921 atcaacaggg atagtcacga tagccacgcc tttatgctca taggcggctt taatggcttc
     4981 atctactaca tgcggaagac tttgtggtgt catgactgta cgattgtata cgctaacatc
     5041 tgcgaaaata ggattttcat ttagttcctg gaaagagtta tagttcatgg atgtagaagc
     5101 aacttgtcct aagaacgcta ctagaggcac atggtccatc tgtgcatcat acaaaccatt
     5161 gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca gcgccgactt tccccgttaa
     5221 ttttgcatct gcagcggctg cgagtgcgcc gacttcttca tgacgaactt gaatgtagtt
     5281 gatttcggtt ttttcttgat atagcgcatc cataattgaa ttgaaagatc ctccgggaat
     5341 gccgtaaata tggtcgatac cccagctttc aagaactttt accattgcga ttcctgcatt
     5401 gatttttgac atggtgtttc actcctttag caatgatgat acataaattt tttgtatcta
     5461 gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc ttttcacaaa aatgtagtca
     5521 tgtttttggg aatatgctag agtagtacat agatagattc aagagagggg aatggcatgg
     5581 ttgaatggct attgagactt gaaccttggc agcaagcact tgctgggaca gcttttactt
     5641 attttatgac agctcttggt gccgggttgg tttttttctt taaagaaatc aaaaaagaag
     5701 tgctaaactt gatgttagga tttgcttctg gtgtcatgat tgctgcaagt ttttggtctt
     5761 tgttagatcc ggctattaca aaagcagaag aaaatggaga tattgcttgg ctagttgtaa
     5821 gtattgggtt tggtttaggc ggtgtgtttt tatatatggc agataaaaca ctgccccata
     5881 tgcattttgg accacaacat gaagcagaag gattgcctac ccatttaaaa cggacgatct
     5941 tgcttgtttt ttctattaca ttgcataaca ttcctgaagg tttagccgtg ggggtagcat
     6001 tcggtgcagc agcaactgcg gataatccga cagccgctat attagccgct gtttcggtgg
     6061 cgctaggtat tggtatccaa aacttcccag aaggagcagc tgtatcaatc ccgttgcgac
     6121 aagaaggtct gagtcgtaaa aaggcctttg tttacggaca agcttctgga atcgttgaac
     6181 caattgctgg agtgattgga gcgctactag taacgaaagt ggagctgctg cttccttatg
     6241 cacttgcttt tgccgcagga gcaatgattt atgtcgtggt ggaagaattg atccctgaag
     6301 cacagcagac attgtccagc aaacgtcatt ttgcagtttt tggcgtcatg agtggattta
     6361 tcattatgat gattttggat gtggcattag gataaataaa aaaaatatat atgatatatc
     6421 aaatagggcc gcgaacttat atcgcgaccc tattttttta gtaagttata tcataatgaa
     6481 gtgaatggta tcatacatta ataatagttt tttctatgtg tatttccttt gatccagcga
     6541 acaagccaga tgataagtgc gattgggatt agaactttga ccgcaaacca taaaagcccg
     6601 cttaggacac tgacaaaaag actgatgacg aagctagctg caattagcac taaaagaacc
     6661 atgaagatat ttaagaacat atttttttga cgcatacaaa accctcctct tgttcattct
     6721 ttactataga aaaagtataa cagggattgt ttaaaaacac ctccaactga agactgattt
     6781 ttacttcatc tacttaaatg atcaaacaat tgctttttct ctggtttatc atctaaaata
     6841 gagcggaaaa ggaggagaat atcatgccta aaaatcgtct tgaagcattt acagatgctg
     6901 taatcgcgat tatcatgact attttagttt tagaacttca tgaacctaac ggagacacgt
     6961 gggaggcgct cgctgcatta ggttaccgat tttttatcta tattgtcagt tttatcattt
     7021 tagcgattta ttggaacaat catcatcatt tatttcaagt tgtacataag ataaatggac
     7081 gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc gctttttccg tttgcaactt
     7141 catggatcag tgagtacatc aatagcctgg ctccagaaat gacttatggt tttgttacct
     7201 tgaccgctaa tattgcgtat tatatcttgg caaaagaact tgtcaaagca aataaaggcc
     7261 acgagaaagc attgaaggcg atgttgaata attacaagaa atcgtatcta tcgattggtt
     7321 tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc agtagccgtg ctgatcgcaa
     7381 acatcgtgat tctagctgct tggatcatcc caagtcgtac catcgaatca caatatcggt
     7441 aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa aaaataaata aacgatcaaa
     7501 tatccgagtg atactagtat tcaaagaggg atgcagatgg atcaaaaaag aattcatgta
     7561 ggactgatta ctagttttgt cacgatgctt atttatttgg atcaaggtca tttgacttgg
     7621 acagtagttg gtgtcgtagg atttttgaca ttattcacag caataaaaaa ataaacatct
     7681 ttatgtaatc tgatgttcta tctatttaag aaaagcatgt ttacagcagt atgattctgt
     7741 tgtaaacgtg tttttatttg tggaaaattt aatttttaga aaagtgtatc gaaaaataga
     7801 tagttagttt ttccacagtg gataacttcg taagagataa aaataaaaaa ttagtcaaag
     7861 aagggcgtat agatttcttt tgataggaga tatacaacag aaatgaataa atattaacgt
     7921 gttttcaaac agtataaaac ggtgtggttt ttggaataaa aaaataacat atctcatttt
     7981 acacttttgt tattttatac ttaaaaaata acgacacaga aaataccgtg tctcagattt
     8041 ctctactact tctaaaaact actgtttttt tcctttgaaa tgccttcaaa aagtaggatc
     8101 aggcagaata tactggcaga atcaaggaga agggagccta aataaggaga aaaaccatag
     8161 aaaatataaa gtaattactt ataaaacaag ctttttatag aaaaataata tacctgagta
     8221 gggtataaat taagacggaa ttcctttcta gattgagata ttgactagga agaatgttaa
     8281 gattttaagc ctttaagcta aaaaaaaacc ctttttgtta aataagggga atatagcctg
     8341 tcagtttcaa actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag
     8401 atttttttaa accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct
     8461 attacggtct ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa
     8521 aaagctacga tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac
     8581 ttatttgtcg atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct
     8641 gttgtattag ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc
     8701 aattctgcca agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc
     8761 ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc
     8821 tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg
     8881 ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat
     8941 aatagaaaga ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc
     9001 tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta
     9061 aggaccaacg tagttatcgt acgaaaatta gaagcttttt ttgttgggaa tccggtttct
     9121 ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc tgtcaaaacg
     9181 actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt gatactaagt
     9241 ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg gattggcatt
     9301 tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg ttcaagactt
     9361 attttcgttt caataaaata caagataatc cttttcgtat catgaatctg atgctactta
     9421 tctttattgt attatggtta ggttacataa atcatgattt tttgatttca agtttttcat
     9481 cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat ttgctgaaac
     9541 gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc ttatcgactt
     9601 atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc agattcgtgt
     9661 tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg
     9721 gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat ttttttatgg
     9781 gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca cggcctgagc
     9841 agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta gtgatcattg
     9901 attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac ggcttgaatc
     9961 tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca gaaaacttgg
    10021 atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg ttatgtgtgt
    10081 cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg atcacttttc
    10141 tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat atttttctta
    10201 atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt tctttgatcg
    10261 aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa
    10321 tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa tagaaggaca
    10381 aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa ttcaaggtgt
    10441 tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa aggaactttc
    10501 aaaaaaattt tatatttatt ctatcaaaaa attttgttta tgatggtaac tgaataaact
    10561 ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga tattctttct tcatagaaag
    10621 tttaagtttt atagttagtg tacaataaaa gtatgtttat accatatggt gtccggataa
    10681 tcagtcattt aaacaatgag gttcaaagat atgaaaaggg gaaatttgat gaagaaaata
    10741 agcaatgaaa tccaaattct ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa
    10801 atgattgtac aagcaaaaaa catccttgat gaggtgtttg agcaatatag tcatgaacta
    10861 atggatccta ataaagcaaa ggcgactgca aagaaaatag acaaagtcta tgaagaggaa
    10921 ccttttgcag cattagctat ttatctatat gcagcttctc aaagttggaa aaaaatttat
    10981 caaccattga agattccaag aactatctat ttggcaacaa tgaatgcttt tactagattt
    11041 attcatgaac atttccaagt aacaggaagc tatcgctttg accgtggttt ttggatttgg
    11101 cattacacta gtggcttgat ctttcggatt ggtgaattgg agtacgaaat gacatatttt
    11161 ccgaaagggt acacggatct aagattagaa ggaaagcagt gcctttctct tcatattcct
    11221 tccgatgcag atttatctca gaataagata agagatagtt acgaaagtgc cattcgtttt
    11281 tttgaacttt attttcctga ttaccattac caagtgatgt acacagatac ttggttacta
    11341 tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat
    11401 tatcgattat tatcagttga tgaacaggac gatagtggag tcccttggat ctttggacga
    11461 gtagatgccc aaatacatga ttatccagaa agtacgagtt tacagcgaca agcaaaagag
    11521 caattgcttg caggcgagca cattggctct ggcttaggta ttcttacttc ataagtttag
    11581 tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
    11641 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
    11701 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
    11761 tcccagcctc ttttttagtg ttctatcgaa cacacagtac gttcaatagg taaactagct
    11821 ataacaatag atattaaagt taacggacaa gagggccttt tttaggtagc taaaacgtaa
    11881 taatgaaaat ttatttccag agataggagt caatcagcta gtctgatcga ctgttttaac
    11941 aagttgactt tcattttaac ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat
    12001 ttgtgttttt tagcatgact acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac
    12061 agatatctca agtgcatacc gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc
    12121 gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt
    12181 aattaaaaaa atagccgaaa aacgtttttc ggctattttc aataatcggt ttttgtgtaa
    12241 ggtttccgaa agaactgctg ttgattttcc atttttaaca tatgataaat tggtagaatc
    12301 tttcttttca aattgatacc attccctaat tccagttata tagttaacta cagaaccaca
    12361 aagcacgtac gtttataaaa gtattatctg tagttatttt tcaattccct ttcaacctct
    12421 tattttgacc agtagtattg atcactttac cttttaacac ctttgttcgt ttttgaaaca
    12481 tttttagtca agaatggaaa cgtaactaca taatcaaagg ttttgcgaaa tatttcatga
    12541 aatatttcat gtagaagcga gcaatacggt tgcaaataac cattatcgtt taataagcag
    12601 ttacgaaagc tcccaatatc tctaacgttc agctcactcc atttcttttc ttcaaaaaaa
    12661 tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa
    12721 aaaatatgca aaaaaataaa aaagcagtta ctattgctag aataacatca aggaataata
    12781 agttttggtt ttcagataat agtcgataag ttgtgtattg gaattttaga tacagtaata
    12841 aagtattttt catgaaattt tgtgagaatc actattacaa gtatctttaa taaaattaat
    12901 ggttaaggag gaacttaaag tagaatgtaa atgaatagtt gtagtcagtc gattgtgata
    12961 ctagactcaa agttgcagga ataagttttt caatttgtga ttggaagata aaaaatgaaa
    13021 aagaagggct tttttttata ctttgttgat tgtagtgttt attttccctc tgtttagcca
    13081 agtaacattt tctatgactc aaccagtttc aagtgaggaa catgataacc tttttcgtgc
    13141 tacagaagag tttaagaaat atcaggaaaa tattgttccg gagactccat tagctagtga
    13201 gccaattact gatattgatg gaaaccaaat aggatggtat atacagtacg aagttatggt
    13261 ggtaaataat gactcaatag taaaaaatac agtaggctta gagctaacac cgattgaaaa
    13321 ggtgagttca ttgttaacgt atatttatac tgatgaaagt gacgagactg agatcatttt
    13381 aacggattac tcaaaaattg ctactgataa aaagtatata ttgtggatgt agacactagt
    13441 gaaatacttt atgatataga aattgatgaa aatttactag atgtagcaca agaaatcgat
    13501 catcaaaagc aggaagctat tcgatatgct ggtagaaaag ggaaaagaaa atatttatta
    13561 cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg aaagaattca agagtcgcta
    13621 ctcaattaac tcgactccga catttacagt ttacagagaa ggatcgatag aaaaaactgt
    13681 tttttggact aaagaaagtg gaatgtcttt agctgaagta gaagagtttt tggattatgt
    13741 tagtatgcaa cagtaaggga gttcttatta tgaaaaattt tgacacatat tatgttattg
    13801 ttttttcgat atctttgctg ttaatattac taactttaat gcagtataaa gagtatccag
    13861 agtattttat tttaactttt gtatatttag taagtagcta cacacttcta tataaattca
    13921 gaagttcttc tagagtttct aaagtttgtt tcttaattag cttattgatt ctataatatt
    13981 tttgatttat ataggaaatt aaaaatatta tagaatcaac actcagagaa cgggttggtt
    14041 tttaatagat ttaataagta ctgataatga aatttctcct ggttgggaaa acgaagttgg
    14101 ttaccctgta gaagaatttt ttcaagatta ctttgcggag tatgccgata ggataatgag
    14161 agattacgta acagataaaa ctcaagaagt catcaatcaa tcccagtcat taaatgaatt
    14221 atctgataag gcgaaagaag aagcagttgc cattatttct tcagggatta gtgactatat
    14281 tgatatgagt ggtgaaatca attatggcga taaagacaaa attgtacgca tgataaaagc
    14341 ttttatcaaa acttttgatg tgaaggcaga tcttaagttt aatgatactt acctagtagg
    14401 aaaattaatt aatatccttg ggacagagaa agatacggaa acgtttattt cctctttgtc
    14461 tatgttgttt acagataagc atttttcaac tcagcttggt ggagaggaac tagtggaaat
    14521 cttttaacca ttacgcccag cattaataaa agtatatcaa aaaagtactc ctgatgattt
    14581 ttaagattgg tttgagaaat aatgtgaaaa cactattttt aatgttaatt atagatagag
    14641 atgaacgcgt catatggtta aaagaatgaa aaagtcaaag atacactgct atatttcact
    14701 aaggatatag cagtgtatct ttcatgtcgt ttcactggca gttttttata aaaggaggtg
    14761 tgcatttcag ttgcttttct ttggtagtta gttgaatcgt ttaagaatta acggatgtgg
    14821 cagacaggtg ctctgtccaa aaaccagtaa aatctttgat ggttccatct ttatttagct
    14881 gtgcctgttc aaatactgtt ttgtttttac cgaatggatc aattgcaata attgttccga
    14941 tccccgcacg tttggcagca agtaatcctg agtaagcatc ttcgataaca agacaatcag
    15001 ccggagctaa gctcagtttg tctgccgctt taagaaaaat atccggttgc ggtttcccag
    15061 ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa ccagcgggct aaatcgaaaa
    15121 tatcaaaata aaaagcaaca ttctctttga cagtagccgt ggcaatcgtt aatggaatct
    15181 gttggtcagt cagctggtca agagtcgctt caagcccttt tgtcaactga agctcgtttt
    15241 cattttgcaa gcaaagcata cgataatagc tttctttttc ttctgacaat acggcgatct
    15301 cagaggcggt caaatgctct gaaatgaaat gtttcaaaat ctccgtattc gtccgaccat
    15361 gaatattttt taaaatatcc tcgtctgata aaccatcttt tgtatgatca tgtatcatat
    15421 gcagccaagc agcttcatgc agatgagagt ccaaaaacat tgttccgttg aaatcaaata
    15481 taatcccttt catagttacg ctcctatagt ggtattttcg gttgaatcag caatttgcag
    15541 atcgacatat tgtgcatagt gctcttgaac ctcccgcgag atctggcgat ccgtaatgag
    15601 cagatcgaaa tcatgcaaat tataaaacac ataaaaatca tctcgattga attttttatt
    15661 atcaacgagt agatatttct tacgggaatt atttagagcg atctgctgtg cttctccttc
    15721 ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca tttgaactga taaatgcttt
    15781 tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt ccgacaaatg cgccggtatt
    15841 tttccggaaa tcaccgccaa tcagtagaat ttctggtcca tctgcttgag caagaatatc
    15901 aaaaactgga taattatttg taatgatccg aattttcttg ttcagtaaat gcgtcgccaa
    15961 tagttcgatt gttgtacctg gtcctaagaa aatcgtttct ccctcgtgaa tcaaggaggc
    16021 tgctgtggca gcaatttctt ttttttcttg aatctgcaga gtggcttttt cagtgtgaga
    16081 aagttcttga tctaaagtgt aggaaagact ttgagcccct ccatggacac gcagcagctt
    16141 gcccgatttt tctaattcat ctaaatctct gcgaacggtc atatcagaaa catctaattg
    16201 attgatgatt tcattaacgg taaggattcc ttttttattg accatttcta cgatagtaag
    16261 caatctttct tttttcaaca ttgtcttcac ctctaattta aggataatct atcatataaa
    16321 cggacaacag tcaatctaat tcgaacaaaa gtaaacattt agggtggttt ttatgtttgt
    16381 tacaatagat tttattgctg ttttctggtt tttatgatta tttgtgtttg tttttgttga
    16441 caaatgtttc ggatgcgttt acaatgcaat cagttagaaa gagcgaaaac tgattataga
    16501 tttgaggaga gatgtacatg cttaaattac cagaagattt tatttttggc ggagccacag
    16561 cagcctatca ggttgaagga gccactaagg aaggtggcaa aggcgctgtt gcttgggatg
    16621 actttttaga ggaacaaggg cgattcagtc ccgatccggc aagtgatttt tatcatcaat
    16681 atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa tggtctaaga ttatccattg
    16741 cttggagccg catttttcct gatggagctg ggaaaccaaa tccagaagga atcgcttttt
    16801 atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac gccgtttgtg acgttgcatc
    16861 attttgatac ccctaaacgt ttgtttgacc atggagattt tttgaatcga gaaacaatag
    16921 aagcatttgt ttcctatgcc attttttgtt ttcatgaatt caaagaagtc aaggtgtgga
    16981 gtacctttaa tgaaatctat ccagttgcaa ccaatcaata tttgttaggt gttttcccac
    17041 caggaatcaa atatgatttt acaaagatcg tcgcctgtct tcataatatg atggttgctc
    17101 atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc gggtgaaatt ggtgtagttc
    17161 actcattaga gacgaaatat gcggcaacag acgcgccgga agataagcat gctgcctttt
    17221 tagacgatgc attgtcgatc cgtttcttat tagatgctac ctatttaggc tattattcaa
    17281 ctgaaacctt gactgctctt gatgagatat gtgaagcaaa tcaggccagc tatcattttc
    17341 ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg taatgactac ttagggatca
    17401 atcactacca atgccatttt gtcaaagctt acgatggaga aaatgcaatc catcataacg
    17461 gtacgggtga aaaagggacc tctgtttata aagtgaaggg aatcggagaa cgtatttata
    17521 aagaaggaat tcctcgaact gattgggact ggctgattta tccagaaggt ctatatgact
    17581 tgttgctgcg aattaaaagc gattacccac attacaacaa aatctatatc actgagaacg
    17641 ggatggggta taaagatcag tttgaagatg gcattattat ggatcagccg agaatcgact
    17701 atttgagagt ctatttggaa tccttaagca aggcgataac agccggggta aatgtgaagg
    17761 ggtatttttt gtggtcgctt atggatcttt tctcatggac aaatgggtat aacaaaaggt
    17821 atggcttgtt ttatgttgat tttgaaaccc agaagcgtta cccaaaagaa tcagcttact
    17881 ggtacaagtt ggtcagtgaa accaaaacga ttatttaata agtgataatg gaggaagaaa
    17941 agatgaataa agaggaatta caaatgcttg gttttgagat cgttgcatat tcaggcgacg
    18001 ctcgcagtac attattgaaa ttattgaaag aagtaagaca aggaaacttt gaacatgttg
    18061 attcggcgtt gaaagaagcc gacgaaaatt taacccttgc acataattcg caaacaaaaa
    18121 ttttagcgga agaagcatct gggaaagaga tggagatggg attcattttt atccatgggc
    18181 aggatcattt aatgaccact ttattgctga gagatctgat tcaagacttt attgtactgt
    18241 acagacagaa tcaaggatag gagaagaacg ctatggatgg aatcattaaa caaatcgaaa
    18301 aagggaaacc attttttgaa aaaatctcac gtaatagtta tttaggagcc gttcgtgacg
    18361 gatttttaac agcgatgcca gctattttat tttcaagcat tttcattttg attgctgcgg
    18421 ttcctgagat ttttggttac caatggcctg aatctgtctc cacatggcta tggaaagtct
    18481 ataattattc gatgggtgtt gtagggttac tcgtttcagc aaccactgct cgctgcttgg
    18541 ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat caacaccacc tctgtgatgc
    18601 tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc gattgaaggt ggattttcaa
    18661 cggaatatat ggggacaaag ggactactgg cttcctttgt cgctgcattt ttaacagtta
    18721 atttatataa gttttgtgtg ctgaaagata ttaccattaa aatgcctaaa gaagttcctg
    18781 gaacgatttc gcaaaccttc cgagatattt ttcctttctc tttttcagta tttgcggccg
    18841 ttttaattga tttaggcatt cgacaaatct tccaaatctc atttgcagaa gcaatcatta
    18901 cggtgctgca gccgctattt acagcagctg acgggtactt aggaatcgct attatctggg
    18961 gagcgatggc gctgttttgg tttgtcggtg ttcatggacc gtcgatcgtg gagccggcaa
    19021 ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca attgttcaat tccggtgaac
    19081 aagcttctaa agtactgacg gtgggtttag ggaattttgt aggaactatg ggaggaactg
    19141 gggcaacatt ggttgttcct tttatcttcc tactctttgc taagtctaag cagttgaagg
    19201 cagttgggaa agcttccttt attccagtaa gtttcgctgt caacgaacca ttgctatttg
    19261 cggcacccat gattttgaac ccttattttt ttattccatt tttgttaaca cctgttgtaa
    19321 acgtttgcct gttcaagttt tttgtcgatg tattgggcat gaacagcttt atgtacgttt
    19381 tgccttgggc aactccagca ccgatcggtt tggttctagg aacgggcatg ggtatcttag
    19441 cttttgttct agcagcggtt ctaatcgtcg ttgacttcct gatctatcta ccattttgca
    19501 aagcatacga tgctgtctta gtagagaaag aagcacaaaa agcagaggtg atcgcaaccg
    19561 aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt ggcagcgagt caagttatca
    19621 gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc tggcgcaggg acaagtgcga
    19681 tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa agcaccgatc agtgcaatgg
    19741 cgggtgctta tggttctcat tacgaaatgc tgcaggattt tgatatggtg gtcttagcac
    19801 cgcaagtcaa ttcttactat gatgatatca aagaagacac cgatcgctta ggaatcaagc
    19861 tggttgcgac aaaaggagca gaatatatcg gattaacgag agatcctgaa aaagctgttc
    19921 agtttattct gaaccaattt caataaagga ggagccataa tgaaaatggg aatttggttg
    19981 cgtaaagctg ttttatttgc agctgtgtta ggggctttgg gggctgtttc aaaaactgtt
    20041 gaagcatctg ccagcgaatt tattcgcgga gcagatattt cgatcctgtc agatatggaa
    20101 aaatctggtg caaaatatta tgaagacgat atttcaaaag atgccttaga gattttaaaa
    20161 aataatggtg tcaactatgt tcgactgcgt ttgtggcagg atccttataa tagtaatgga
    20221 gaaagttatg gagcagggac aaatgatctg gaaacgacga ttgaccttgc aaaacgagca
    20281 aaaaatctgg gacttaaggt gctgctggat tttcactaca gtgatttttg ggttgatcct
    20341 gggaaacaga atcttcctaa agcatggcaa ggtctgacct ttgaagaaat gaatacggca
    20401 ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc agctggacgt ttatcctgat
    20461 atggttcaga tcggcaatga actgaatagc ggaatgctct ggccttatgg caagtcttgg
    20521 ggagaaggcg ggggagaatt tgatcgattg gctgcttttt tgaagtctgg tatccaagct
    20581 gttcgagaca ctcaacctaa aacaactcca gtaatgctcc acttagctga tggtggcgat
    20641 acaggagcat ttacttggtg gtttgatgaa atcacgagtc gaggtgtgtc atttgattta
    20701 attggagtgt cgtattatcc gtattggcac ggttcaatga atgaacttca aaccaatatg
    20761 aatgcgatca gccaacggta tggtaaagaa gtgatcgtag ttgaaacagc ttatgggcat
    20821 acaacagcga atgccgatac tatgccaaat gcttttggtg aagccgaagc agctgcgggg
    20881 ggatatgaac caacaccgac aggacaagca gagtatttgc tggatcttgc cgatcggatt
    20941 caggcagtac cgaataatcg cggggcgggc ttcttctatt gggaacctct ttggtacaac
    21001 ggaaatgtga gttgggcaac acaagcaggt atgaattatc tcagtgttca atccactatg
    21061 ggaaacgaat gggacaacca agcgatgttt gattttgctg gaaacgcctt gccaagcttg
    21121 cgtgcgttta aacaagcagg ggcgcaaata aatctagtta aaaatgcaag tttcgaaagc
    21181 gatggaccaa cgaccagtcc ttccaattgg caaacctggt ttcaacaagg aaccgctaag
    21241 ggtacgatca aaacggaagc gggcactttt tttggggact acaaactaac tttttggcag
    21301 gatcaagcat acgaggcatc agcttatcaa aatgttagtg gcataagcaa aggaacttac
    21361 acattgagtg cctatgtaat gtctggggga gagtttgata ctaatcagtt atatatcaaa
    21421 aattatggtg gagcggaact aaatactgcg gttgttaaaa caagtgaatg gacaaaaatt
    21481 acaatcaaag acattccaat tacaacaggt gtttgtgaaa ttggggttta tagcaaggct
    21541 tcaggaaatg cttggacgaa cgttgatttt gtttcattgg tgaaacaata gactgcggat
    21601 cgtttcgatt caagatcgga ggttcccttc agccacatcg ttagcgtaga aggcaggaat
    21661 cgaacctgca cctcgccttt gccaaaaata accagaaacg atttccagtc atttttacca
    21721 aaacgagtaa agcaccagac ttcatctaca ctttgtatta tgctaaagag agttcaacga
    21781 tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca gataacataa agtttgttta
    21841 agaaagggta accttagaat tccgtcgtga atatgatttg agcacttaga tggatagaaa
    21901 aaagttctcg gaaaacagtt cgtttttcga gaacttcttt ttatgataag aatgaaacaa
    21961 attaacttat attatagtct cttttggtag ggaaccatct tatttttaat gaaaatcaag
    22021 cattttagct attgaagaat gttgatttat agggattccc cctccatatt atgttggctc
    22081 tttattccca ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga
    22141 cgtgggctac ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac
    22201 gggcaaagtc agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt
    22261 cgtaagtacg tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat
    22321 attgccagat atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag
    22381 attcgcgtac gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac
    22441 caggaccagg gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta
    22501 attcacggac ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat
    22561 cttctggcag tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag
    22621 attcgataac gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag
    22681 caagttttgt tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt
    22741 tttgttctgg gtcagataca cctttcaact tgtctaagaa gcgatctctt gcattgactt
    22801 tgatgatgtt caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt
    22861 tgcgtagcag accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta
    22921 aaagaacccc aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt
    22981 caccaacttg ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt
    23041 taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg
    23101 aatgacgtac ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag
    23161 aagcaatcgg acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta
    23221 ccaagtcacc atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag
    23281 cttgatcgct tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta
    23341 cggaaccgcc aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa
    23401 tacctaaatt gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg
    23461 gtccgccaga aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc
    23521 ggtggctcaa taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt
    23581 tatactgact accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt
    23641 ttgtcaaatt tattacccct atccctttaa attttatctc aaaatagtca aactatttta
    23701 aaatgctaat attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga
    23761 attatatcgg ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca
    23821 tttttccata catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga
    23881 taataataat tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa
    23941 taccattttt cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg
    24001 ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag
    24061 atatcaggct gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag
    24121 taaacaggta cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc
    24181 atctccatat cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat
    24241 atacgatttg gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag
    24301 gtacgagaca aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt
    24361 ccggcgatcc cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta
    24421 ctcaagaaca aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca
    24481 tagatatctc gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt
    24541 tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc
    24601 acacggtagt agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa
    24661 ctattttaac acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg
    24721 tataatcttt ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa
    24781 aaggatagac tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact
    24841 attctgaacg cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca
    24901 gaacctttca atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt
    24961 cacgtgtcct gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag
    25021 atgtgggatg cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg
    25081 ttgaaatggt cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta
    25141 accaaatcga acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa
    25201 cctatgcagc tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa
    25261 ttttgacagg cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga
    25321 agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa
    25381 tcgtcacaaa agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa
    25441 aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt
    25501 tttttgttga gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg
    25561 aacagagtcg ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg
    25621 ccaagtcggt agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc
    25681 ttttcttggg ggttttggta cactgatagg gaatttagat agaagggagc caataggatg
    25741 aagctgacca tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag
    25801 attttgaata acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc
    25861 gtgaatgaac atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca
    25921 aagaccatcg gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa
    25981 ggtgtagaga gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat
    26041 agccaagaaa atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc
    26101 ttgctggcca caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa
    26161 aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc
    26221 gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg
    26281 aagatcggca tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga
    26341 tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga
    26401 gagttgactg tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc
    26461 acggctattt tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa
    26521 ttgggcaaaa aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca
    26581 gaatacatga ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat
    26641 gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg
    26701 ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc
    26761 ggtatattgc ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa
    26821 accagtttac taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca
    26881 aggaggatca ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc
    26941 tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt
    27001 atcaagtcag tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca
    27061 cgaacttaac attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag
    27121 gaataaaggt agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag
    27181 cccccctagt agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt
    27241 acacaggtgg tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg
    27301 atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa
    27361 acttggcacc ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt
    27421 ctcttccgct gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa
    27481 tcgttttgga tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac
    27541 gtctggcaaa gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta
    27601 tctcagatat gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg
    27661 caatcgaaac attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag
    27721 gaagccaaat ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc
    27781 ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc
    27841 aagggggaga cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa
    27901 tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa
    27961 ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg
    28021 ttggattgaa actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga
    28081 cgatctatag taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg
    28141 aaattggtga atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga
    28201 aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga
    28261 tcgaccatac tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag
    28321 aagcaaaaaa ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga
    28381 aagaacaatt acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag
    28441 caaacacacc cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg
    28501 aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa
    28561 aagatatcga agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattgttg
    28621 aaacatcgtt attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag
    28681 gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg
    28741 tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc
    28801 ataacgaaga agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg
    28861 caggagtagc aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa
    28921 gctcatggca gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc
    28981 ctatgttcct tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat
    29041 ttatcaaggc atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac
    29101 agcttttttc aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg
    29161 gcacaccccc gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg
    29221 ttcccctgat atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt
    29281 tcgtgcactg ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc
    29341 agctgactgt tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca
    29401 aaattatttg gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc
    29461 aacaacggct ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca
    29521 ttgatcacag acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga
    29581 ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc
    29641 caatcttcaa atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc
    29701 aaaacgattt ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac
    29761 aatacaggta cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta
    29821 tctgcaactt tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg
    29881 actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt
    29941 gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa
    30001 gtattgaatc aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa
    30061 ggtatgtatg cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt
    30121 gtattccaag aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt
    30181 gttgaccgcg accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc
    30241 acattgacat ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca
    30301 gcggacggta aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt
    30361 ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa
    30421 aaaatcattt caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc
    30481 tatagccttg ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc
    30541 atttatccaa gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt
    30601 gtttcaaaag aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa
    30661 atcgttagga tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat
    30721 taccaaagaa tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg
    30781 agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct
    30841 ttccggatta ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat
    30901 cacaagtccg accaaagcca accaattagg gattgggatg gtccatcagc actttatgct
    30961 tgttgatgcc tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg
    31021 gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt
    31081 gtcagtcaac ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga
    31141 aattttaaaa acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt
    31201 attgacccct caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg
    31261 caagtcgatc attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg
    31321 tacagttatc cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca
    31381 acaattagcc gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc
    31441 gaagcctcaa gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt
    31501 agaagccgtg aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg
    31561 aatcgatgga aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga
    31621 aagcggacat atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac
    31681 tgaacatggt gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat
    31741 gacattatct gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg
    31801 catgctgaat tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt
    31861 tcgaacaacg aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc
    31921 aattatcgct cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg
    31981 tggactggat gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa
    32041 atacaaagca gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg
    32101 tattgctgtt atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga
    32161 aaatgaatta ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga
    32221 aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg
    32281 tactttcagt tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc
    32341 agcccatcca aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag
    32401 gtgaaacgtt aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta
    32461 actcagctgg gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca
    32521 gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg
    32581 tgatcttagg tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat
    32641 tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa
    32701 gcacatttgc tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca
    32761 atatgatcag tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct
    32821 ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga
    32881 tgaagaaaac gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg
    32941 agtatgcagg aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt
    33001 tggcagggct tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa
    33061 cgacttcgct atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag
    33121 caatcggtat tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca
    33181 tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct
    33241 tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa
    33301 aacaagaaga gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg
    33361 gcgaactata atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta
    33421 ttcaacaccg ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt
    33481 aaacgtaggg ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt
    33541 gacgatgagt ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt
    33601 cgtaggtatc ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca
    33661 tattatcagc gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa
    33721 agcatggtat ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt
    33781 cccaggatta gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc
    33841 tgcttattgt gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt
    33901 cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa
    33961 tgtatatgct atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg
    34021 tgctgttttt gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg
    34081 gttcatctcc atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc
    34141 ctcattgttc tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat
    34201 ttcttcgatt ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct
    34261 ggttgttttc ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc
    34321 aaaataatct tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct
    34381 ttttgtttta aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta
    34441 catctgatcg tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt
    34501 gatgttggtt ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat
    34561 ttagagcagc ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat
    34621 catcaaggct atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc
    34681 cactgggaaa tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc
    34741 cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa
    34801 ccttatagcg gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat
    34861 ttaattgtat atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt
    34921 cctttagaag aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg
    34981 tatatgattg gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga
    35041 acaagcaatc gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg
    35101 aaagaagcag gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa
    35161 ggaatcactg aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa
    35221 gttatgaaaa acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta
    35281 tacggtcatc gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga
    35341 attccagaat tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac
    35401 ggaaacgatc cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt
    35461 tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca
    35521 gcaacgattg ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa
    35581 ttattaaaat aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac
    35641 aagaaacgac ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc
    35701 taggttcagg attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg
    35761 atgagatccc gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg
    35821 ggtctctggc aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc
    35881 attccatgca aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag
    35941 tcattgtgac taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc
    36001 tgattaccga tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg
    36061 caatgggccc tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag
    36121 caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt
    36181 ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg
    36241 cagtagggat gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt
    36301 taggaatctc ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag
    36361 aagttgtaga aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat
    36421 tgacattatt gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc
    36481 gagatcgccg ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag
    36541 acttttttgg aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc
    36601 aaatataaag gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc
    36661 atctatgccc atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca
    36721 tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg
    36781 accagttctt cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat
    36841 ttcaatctat tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta
    36901 ggaaatgttc tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta
    36961 gctgaacttg gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg
    37021 aaataccatg tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa
    37081 gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa
    37141 acagcgatag cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca
    37201 tccaagcaaa aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa
    37261 gcttttgaac accgtttacc aagaggttgt gaa
//
LOCUS       DACELF010000017        36939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.017, whole genome shotgun sequence.
ACCESSION   DACELF010000017 DACELF010000000
VERSION     DACELF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.017"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..523)
                     /locus_tag="IW687_000837"
     CDS             complement(95..523)
                     /locus_tag="IW687_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950119.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(551..1189)
                     /locus_tag="IW687_000838"
     CDS             complement(551..1189)
                     /locus_tag="IW687_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950120.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1193..3229)
                     /locus_tag="IW687_000839"
     CDS             complement(1193..3229)
                     /locus_tag="IW687_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0950121.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3355..4020
                     /locus_tag="IW687_000840"
     CDS             3355..4020
                     /locus_tag="IW687_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950122.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5955..9104)
                     /locus_tag="IW687_000841"
     CDS             complement(5955..9104)
                     /locus_tag="IW687_000841"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0950123.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9117..10316)
                     /locus_tag="IW687_000842"
     CDS             complement(9117..10316)
                     /locus_tag="IW687_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0950124.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10306..11901)
                     /locus_tag="IW687_000843"
     CDS             complement(10306..11901)
                     /locus_tag="IW687_000843"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0950125.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12485..13192)
                     /locus_tag="IW687_000844"
     CDS             complement(12485..13192)
                     /locus_tag="IW687_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950126.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13257..14093)
                     /locus_tag="IW687_000845"
     CDS             complement(13257..14093)
                     /locus_tag="IW687_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0950127.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14111..15520)
                     /locus_tag="IW687_000846"
     CDS             complement(14111..15520)
                     /locus_tag="IW687_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0950128.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15534..17357)
                     /locus_tag="IW687_000847"
     CDS             complement(15534..17357)
                     /locus_tag="IW687_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0950129.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18200..18526)
                     /locus_tag="IW687_000848"
     CDS             complement(18200..18526)
                     /locus_tag="IW687_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950130.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18572..18934)
                     /locus_tag="IW687_000849"
     CDS             complement(18572..18934)
                     /locus_tag="IW687_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0950131.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18971..19186)
                     /locus_tag="IW687_000850"
     CDS             complement(18971..19186)
                     /locus_tag="IW687_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950132.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19313..19648
                     /locus_tag="IW687_000851"
     CDS             19313..19648
                     /locus_tag="IW687_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950133.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19724..21721)
                     /locus_tag="IW687_000852"
     CDS             complement(19724..21721)
                     /locus_tag="IW687_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950134.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21819..22094)
                     /locus_tag="IW687_000853"
     CDS             complement(21819..22094)
                     /locus_tag="IW687_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950135.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22326..23060
                     /locus_tag="IW687_000854"
     CDS             22326..23060
                     /locus_tag="IW687_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950136.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23099..24241
                     /locus_tag="IW687_000855"
     CDS             23099..24241
                     /locus_tag="IW687_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0950137.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="IW687_000856"
     CDS             complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="IW687_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0950138.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25732..26202)
                     /locus_tag="IW687_000857"
     CDS             complement(25732..26202)
                     /locus_tag="IW687_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0950139.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26189..27424)
                     /locus_tag="IW687_000858"
     CDS             complement(26189..27424)
                     /locus_tag="IW687_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0950140.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="IW687_000859"
     CDS             complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="IW687_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0950141.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="IW687_000860"
     CDS             complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="IW687_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0950142.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29690..30538)
                     /locus_tag="IW687_000861"
     CDS             complement(29690..30538)
                     /locus_tag="IW687_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950143.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30562..31248)
                     /locus_tag="IW687_000862"
     CDS             complement(30562..31248)
                     /locus_tag="IW687_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950144.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31241..32275)
                     /locus_tag="IW687_000863"
     CDS             complement(31241..32275)
                     /locus_tag="IW687_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0950145.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32670..33008)
                     /locus_tag="IW687_000864"
     CDS             complement(32670..33008)
                     /locus_tag="IW687_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0950146.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33010..33366)
                     /locus_tag="IW687_000865"
     CDS             complement(33010..33366)
                     /locus_tag="IW687_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0950147.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33372..34559)
                     /locus_tag="IW687_000866"
     CDS             complement(33372..34559)
                     /locus_tag="IW687_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0950148.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34750..35595)
                     /locus_tag="IW687_000867"
     CDS             complement(34750..35595)
                     /locus_tag="IW687_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0950149.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35829..36794
                     /locus_tag="IW687_000868"
     CDS             35829..36794
                     /locus_tag="IW687_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0950150.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10954 a         7582 c         6122 g        12281 t
ORIGIN      
        1 tacaactaaa aatgaatata ttaaaactac caaaaataac ttttaacgct gtttcattat
       61 atgttttttc tattttatat aaacttttgg atacttaata ccaaccggtt ggattgtacc
      121 aacatgagaa tgtgtcagtt ctatttcctg tcgcacttgc ataggcccat actccttttg
      181 ttgcatttgc tgatcgaata gagtttccaa ctttcgcttt tgcaccgtga cgattataga
      241 cacgactata gtggttagaa tgacctgttt ttctcgtaca agttgtatca ctataataaa
      301 cccatcttcc agagctatga ttaattgctg ttctagaaat ttcccggtct actggtaaat
      361 ttgacttaat aatattacct ttagcatcaa aatgaatctc tcctggcttt aaagatttgt
      421 aatagatttc accgtgaact gaatttgctt ccacttcaga tgacatagcg tttcctccaa
      481 caaaacctat ccctagtgca caaataataa ctgctttttt cataaaacga catcctttcc
      541 tttttgattt ttaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg
      601 tgactacgat aactaacgta ccttgggaag cttgttgcga taagatttca actactttat
      661 ccttatttct tttatccaat gacccagtcg gttcatcggc taaaatgatt ttgctattct
      721 tcaataagac tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag
      781 gcgtacttag cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct
      841 tatctgaaac atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt
      901 catattcaat caacccaaaa tcttgaaaaa gaaaagatat ggtatttctt tttatcaagc
      961 gcttctctct cccagttaat tgtttcgtct cttgatcgaa caaatagaat tgtcctgaac
     1021 tgaactgatc attcaatcct aatatattca ataacgtact tttacctgct ccactttctc
     1081 caacaacagt cacaagatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt
     1141 tgtttcgaat gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc
     1201 cattcatcag caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa
     1261 taaaatcaat gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa
     1321 gaaaaaggaa gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt
     1381 tatacgtttc aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt
     1441 agacaaaata tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta
     1501 aaaatatctg tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag
     1561 tctgagtata cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct
     1621 caaataacct ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc
     1681 cgctgagaat atttctttcc aaatacatac tgtttttcaa tgtcagtatt tcaacaaccg
     1741 gataatgttc gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta
     1801 taaatagaaa tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca
     1861 ggtcattttt taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt
     1921 ctttttcggt tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat
     1981 aattcagatt tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg
     2041 atacattgac taatatactt ccgtttttat tcaaagtttc atataattcc tgatctgctg
     2101 ctgatgcaaa acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat
     2161 aaaatacatg atagtcctca ccagcgattt cttttggtgg ttcatgggaa acagctaact
     2221 gtgaagaaga aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa
     2281 acaacaatcc aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag
     2341 cgattttttc aactaataaa agagaaatca aagagatacc atatattccg ctgaacaaga
     2401 aaacgtacat ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac
     2461 caatgattcc tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca
     2521 atacttgatg taatgaaaag tcattattca acaataaact gatttcttta ttcatcaata
     2581 agaaaaagga tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat
     2641 aattatatac gtctgtattt gaaataaccg aatacgaata gctattttca aagtcttcaa
     2701 aaatattagt ggcataatca gtgtgaaatg cttgattgaa tcgatcagtg aatagtgaaa
     2761 tgacccgttc ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt
     2821 ttattttgat cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct
     2881 tctcgtgaga agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta
     2941 tcttgaagtt ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag
     3001 aaagctcagt ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt
     3061 gcccaataaa atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt
     3121 catttgtccg atagagtacc tgtttattta tactaaccaa ggcaaaacca ctcatgatac
     3181 cggtcactat gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt
     3241 acttaaatga ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaag
     3301 tgtataggtc atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac
     3361 accggagaaa aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc
     3421 gaaaaaattg gtgtttccga taaaacgatt tctagttggg aaaattctcg tacaatgcca
     3481 gactttgaaa tgctgtcact tttacatcat actttaggtc tgccgattga ttttccttct
     3541 attgaatcag ctaccgaatc aatagaacca acattgccag atgaacctct tgttaacaag
     3601 aaatcttatt catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc
     3661 gtcttttgtt tggtctatct ttttcttatc aattatcaat acaaaaatga aacgatcgat
     3721 cgattcaatc cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg
     3781 gacaattcca tcgaacgttg ggtatgtaat gatatctttg gccatggtac atggctgtct
     3841 tttcacgctg gagaggtaga aaaagagaaa aaccatgctg taatcaaaca caaaggaaaa
     3901 cacgtcatga acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc
     3961 ctcagccaag actatgacga cgccttaaaa agaagccctc aggagccaga taggcattaa
     4021 gtaggagatg aacgtagaga tagaaaaaat caatgcacat gatcttatcc aaaaaacagg
     4081 ggtacaaatt aattgaaaaa gccttgagat gaaagttcct aaataaaaaa gttcagaatg
     4141 ataggtattt ctggcttata gtataatatt tacgccacag cctcccaatg attctttttg
     4201 gaatcataat caaggatgga cattcacaaa aatattttgc tcattcgatc cgaatagatt
     4261 aacgaccaat ctagtaagta atatagattg gtacgtagtc aagctgcaca taatttagat
     4321 caattttagt tcttttttat atttctatga tctgctcatt tacttttata tgatggctta
     4381 cagtaacata aataaatatg ataatcgctg gtagtttatc caagagtact caataaaata
     4441 caaaatggat tgttcaagcg cctatcataa tgataaaatg aaacaaataa acgatacaag
     4501 gaggaagaga ctttgagtaa tggcagaaca attttttcat tgtaaaaatt gttcagttgt
     4561 taatctatgg gatttcggta gcatgggcat ttttttatag aggaatcaca ccaacctggc
     4621 cgttttatct cttgttagtg ggaatattag taggaaattt tgcccctctt cactatcgta
     4681 cgaacttttt tccaactgat tctggccttt ttcttaaaac aaagacgcga aaattagaaa
     4741 acattattga aatgacaatt acctgtataa tcattttctt agttatattc ctaaacgtct
     4801 tctaataaga atctcttaaa attagcttgt tacagttagt tacctagttt atataacaca
     4861 gttatcttat cttccttatc aacatctttt gccaactctt tgtcttaaat tcgaaacata
     4921 taagcagagc tacgaacacc aagactgttt cggctctgtt ttttgcatca aattcactct
     4981 tatatagctg ataatctcgg ctctttaaaa gaaattattt tcgcttcatt ttattataaa
     5041 aaggctagga accttgatgt tatcaggttc ctagccttta tttaagtatt accccacaga
     5101 cccttccata tagattttta aattacacac tagtttaaac taaaacaaac aaggaaaaac
     5161 ttaccattta atcaaaaaat tagctcatat gaatatatta gaatgtaaat tagactggtc
     5221 aaaatctggt caaaaaagag gacgaataca ggtagtcctc ttttttgtta ttttgatatt
     5281 ctaaatcatt ttttattgga ttattccctt gttcgatata gacccaaatt gatctatcaa
     5341 taattatcaa actgagtcat atagagacaa aaatttcaaa aatgacctgt taaatggtca
     5401 tcgaatatca tttaaaatca atacgacgga gtcagacacg gagtcaaaac aagcccgctg
     5461 agagtaaaaa atatacagtc ccataggtaa agtgccacag gactgtatat ttttttattt
     5521 gagattacct gaaatgattc catttaattt ttatctcatt ttttcctact agttagtatc
     5581 aaatactctc ctattttctc aataaactat tttgggttac atggtatctg aaacagctaa
     5641 aaccttgtca ttatagcaaa aaatcacttt atctccgttt catttcagat acatttcact
     5701 taagcggatt ccaaaaaaac aaaagaaaaa ggaaccccta gttagcctat gctttttatt
     5761 ggttcctctc aaaacacggt catataacaa cggggtgggg taaatgaatg aaaaaggtgg
     5821 aggaaacatt attcaatagc tgtcttaaat acttcgttat aaaatgtgaa caaataaaag
     5881 acatctatcc ctattttcta cacttccact tgactaccat ctttgaatcg tacaattttc
     5941 ttaactcttc tcgtttacct caattcattc tcaagtggaa tcaactcatc atctaacatt
     6001 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gcttcgcttc tggatgtttg
     6061 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
     6121 tcacctggta gatattgcag attagaagca tgtaattctt tctcagaaac aaaccactca
     6181 ttggaaaaga catgaatcaa atcatcactc gcttgtgtaa aatagcgacg tttaatgaca
     6241 aagatatctt cattactatc taaagtcccc acatcaaatg catttaaaat ttgtcggtaa
     6301 acttctttga ctccttctgt tttattcaat ttagccaaag ctttttcaat ttcttctcgt
     6361 gcatctgaac tattcgcagc ataagttcct agtaattgat caatataagt tgaatcaata
     6421 tcatacaact tagtggtatt ttcactataa aattcgatcg ttgaaaaatc ttctccttct
     6481 ttttctggtt cttcctctat taatgaaccc ttcacggtct cgtacacgcc aatttgttct
     6541 tccaaaagtt gaatttgatt gttcaaagtt ggagatttca ccatgtcatc attgtaatcg
     6601 tcataagtca ctaacgattc ataagcacga ttcaattctt gaaatgcttt gacgtactcg
     6661 atacggattt caagtggcgt atgttcatca atggattcca tggatgaaac ctcttttaac
     6721 ttaccatatg cttgtttgaa tctttttttg gattcctgat aacttggata aatcaaacct
     6781 gattcttctt ttgcttttga atacagtttt gtcgcatcct cgacattctt ctccattgtc
     6841 gctggcttac gaaacgtcac aatcaatcct tttgtcttat caggatacgt acgattcgta
     6901 cgtgaaaaag cttggatcaa tcctgcatac tctagattac gatccacaaa tagggtttga
     6961 atcgtagggg cgtcaaaacc tgttaataga cgatccacta caatcgctaa atcgatttgt
     7021 ttcccaaatt gtttgaattc cgctcttttt cgtgccaaac gattattgat gtctccgtta
     7081 taacgctcaa tgtctgctaa tgaccaggaa gtatcataat actgattata ttcctcaata
     7141 attttttgca tttcttcttg ttgggcactt gaatctcggc tattttcttc taatgaatag
     7201 gtaatagcga ttcgtggaaa atcaggatcc tccatcgttc gtccttcacg aatgagctga
     7261 ttgggaaact cttttgttaa ccaatcaggc tctttcgtca tctctttgat ggcccgataa
     7321 tactttttgg ccatgtcaat ggaactcgtg gttaagattg cagatttcgt tggcctgcca
     7381 tttcgaaaat caaattttgt ataggcatta tctggacgga acattttatg aatgactttt
     7441 tggatatgtt catctgcttc aaaaacagct gggtccaaat aactttcttt tttgacaggt
     7501 tccatttgat caatcatacg attgatctgt tctgatgaat actctgcata tgtttctact
     7561 tcttttaatt tccgatagat tttattctct agtgaagtcg gctccacggt gttttcatgc
     7621 tctacttgaa atcctaagac agagccatct tctagcgcat tcttaatggt ataggtatgc
     7681 aagacctctc catactgatc atgggttgta cgtgccaatt gccctttggc ttgtttccga
     7741 ttctcaggaa aaatcggtgt acctgtaaag ccaaaccaag tggatttcgg aaacattttt
     7801 ttgatttctt ccatattttc agcacttaac gctcgatgac actcatctac tacaaacacg
     7861 atatgttggc ccattaattt ttggaagcga ttggttcctt ttttctcttc ttgtttcttt
     7921 gcatatttca acgccgcatc tagtttttga cgggttgtaa tgattacaac atttgcattg
     7981 gcatctgcca acagggtttc acttaattct ttggcacttc ccgttccaac gatcaagcta
     8041 ttcgctttgg cgttcccgga agaaatccca gtattaaatt ccgaagcaaa ttttgtaaac
     8101 tctgtggtgg tttgattatc taaatctttg cggtccacta acatgatcgt tcgatcgatc
     8161 cccgattttc gtgctaaaag cttagtagat acaaaactag tcaacgtctt tcctgaacca
     8221 gtcgcatgcc acacataccc tgattgatgt ttatttgcgg caataaacaa ggcttgaatc
     8281 gcatgtactt gataaggatg taacaccatg agggttttat tatcttgatc ttcactgacg
     8341 attgtataat ccgcaatcaa acgatgagca tccgggatat ttaacacttg tttacaaaac
     8401 tcatataagt tttctacttt ttcattgtct ttcgttcgcc aactaaataa aaacttaggg
     8461 tgcaagtctt tcggtaaggc atttgcaaaa taccgagtgg tttgttcatt ggaaaccaca
     8521 aacaactgta aggtcgaaaa gatattgttc cgaaacatac cttcttctgc atattttttg
     8581 atttggttaa atgcctgata aacaccatct tttgcagtta cttgtttcag ctcgatttgg
     8641 acaatcggta atccattgat taataaggtg acgtcaaaac gtcgatcccg tgcttctatg
     8701 ttacttccac gttttgcaat ttgatggacc acttcataag tagagattcc tccaccaatg
     8761 tcttgattgg aataaaggat aagtgagaca gaacccaatt gcgaatcttc ccgttcaatc
     8821 gtgatccgag ccatcccatt ttcacctttt aaccatttcg cagcatcaaa tggggttttc
     8881 gttcgcagca gtaattccgt tttgatcgtt tcaaattctt tatcggtcaa aggaaaatca
     8941 ttcagttctg cttgattatt tgagataatt ttttggcgta aattgaccca aaggtcttct
     9001 tctgatttta gatccggacg atacgtccac tgattatgcc cttctccaag tacacgaatc
     9061 aactgttctt cgacttctgc ttcctcacgg tgactaattt tcattcttct tccctcctag
     9121 acaaacatct tttgtaagaa gctttttttc gtttctttta gtaaatctaa cttacgttga
     9181 tgaagagcga tcgtatcatc tagctgtttg aagaaagtac cgattttttg ttgttcatcc
     9241 tttacaggaa ccttaacact gatattctca atatcagaac tgttataaga aggcaatgcc
     9301 cctacttggg agtatcttgg caaatttata gtttcaaaca atattttacc aaaatagata
     9361 tcccaagtat tattaaataa ataagccatg gtattgttat ctattaagaa agggcttgtg
     9421 accaaacgtt ttctattaag cattattgct gcgccaactt tagcaaatat aatcgctggc
     9481 acatctttta ttggcttcca ttttttacgc tcaatttgct cattagatac ataatgattt
     9541 gcatttctca tttcatactc attaccaatg ttattcatgt cggaaacttt aaaaaatgga
     9601 gtaccttcag aaccaccttg ctcggcatca ggaaatccaa ttcccgattg agttttacct
     9661 aattctccca acttacgctc ttcccaatct tccgtaaaac caggaaaccg gatttctggc
     9721 acttttgctc cgttttcagg gaacattttt tgtaagaatc cttttttcgt ttccttcagc
     9781 aaatctaact tacgttgatg aagagcgata gtgtcgtcga gttttttgaa gaatatgccg
     9841 attttttgtt gttcttttaa atcattggga atatagaata ctgtatctga aacttttttc
     9901 cagtctgaac gaggcatttt tgttccagat gtatcatttg cagccttttg atatctatca
     9961 gcttgaataa gagaataaat aaagtccgag tcgctcttct tagctctaaa aacccagaaa
    10021 tccccaatcg ctacgcctgt aaaagttgcc ttgagccaat tttttaaata tggacgtaac
    10081 tttccatata gaatatcatt tggtaaaaat tcaatccctt ttctattatc aaatttctgg
    10141 ctcacatctt tattcagtct accttctcct gccacgatat cttcaaattc aacttttgga
    10201 agtctactgg agtttgtact tttattaagt ctaacaacta aatcaattaa cttacgctct
    10261 tcccaatctt ccgtaaatcc tgggaaacgg atttctggtc gtgtatcatt gctcatgttt
    10321 gaacacctcc agcgcacctt gtaaccacat ttcgttttca ggtgttgttt gcagggaaga
    10381 gatagcttct aaaagttctt tttctagcac ttgctgttct tgtcgtatct tcttgatgtc
    10441 atttccgaca tgaaccatat caattggttc ttcttcttcg aaagtatcta catagcgtgg
    10501 gatatttaag ttatagtcat tttctttgat ttcatcaaag gttgccaaat gagcgtattt
    10561 ctcgacatct tttctttcag cgtaggtttc gaggattttt tgaatgtttt cttccgatag
    10621 tttattttga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
    10681 agtttgacga ttcttcttca acacaatcac cgtcgttgga atcgatgtcc caaagaacaa
    10741 gttcgcaggc ataccgatca cagcataaat actgccatct tctagtaatt tttgacggat
    10801 gaccccttct gcagcgccac ggaataaaac tccgtgtggt aagacgatgg ccatggtacc
    10861 tgtttccttc aaatgataga acccatgaag aagaaacgca aaatctgctt tggattttgg
    10921 cgctaatttc ccataacgat taaaccgaga atcatctaag aaagtcgtat ctgctgacca
    10981 attagccgaa taaggtgggt tcattactac tgcatcaaat gtgtaaggtt catctgttgg
    11041 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcgt cgacaccatg
    11101 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
    11161 tttaacgtta tctgggtgtg ttaagtagtt acgtacgttt aacatcaatg aacctgaacc
    11221 catcgttgga tcaaaaacac taaagaatcg tcgttccttt tgatcgagcg ttacgatttg
    11281 cgccatcata tcagaaacca tatgaggtgt gtaaaattct ccggctttct tccctgcttc
    11341 tgaagcaaat tgactgatca gaaactcata cgcatccccg atcacgtccc catcgtgttc
    11401 taaaacatct acatcattca atttcttgat gacttcggta atcgtcacat ttcgttgttg
    11461 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
    11521 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
    11581 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgcaattg catatcccaa
    11641 aatatcaacg attgtggcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
    11701 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
    11761 aaccacttgt tccaacaatc gatcggataa atatttatag aaaatcagtc ccaataaata
    11821 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
    11881 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
    11941 ccctcttatt ttagcatacc cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt
    12001 cttgctaata aactacgcga aaggagtcta acgttgtttg atataactta gactgatcgt
    12061 taacttattt tttatatttc tacatagtta tctttagaac catttcagat acatatgaaa
    12121 caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa
    12181 tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc
    12241 aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca
    12301 aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg
    12361 tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa
    12421 cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat
    12481 ctttttaccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg
    12541 cattactgat acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa
    12601 tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg
    12661 gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat
    12721 ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa
    12781 ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc
    12841 catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag
    12901 ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat
    12961 gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc
    13021 ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct
    13081 gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt
    13141 cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag
    13201 gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag
    13261 tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc
    13321 atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc
    13381 tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt
    13441 cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca
    13501 accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc
    13561 gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca
    13621 atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat caattggata
    13681 gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga
    13741 ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag
    13801 gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt
    13861 cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt
    13921 tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc
    13981 aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct
    14041 tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct cattagattc
    14101 accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt
    14161 ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac
    14221 cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat
    14281 aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta
    14341 gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc
    14401 catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg
    14461 ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat
    14521 tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag
    14581 cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa
    14641 tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag
    14701 gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg
    14761 ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca attgcatctt
    14821 ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt
    14881 taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat
    14941 taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc
    15001 caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac
    15061 catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta
    15121 accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg
    15181 gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt
    15241 gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag
    15301 cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg
    15361 aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt
    15421 gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata
    15481 aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta
    15541 atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa
    15601 ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca
    15661 gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat
    15721 acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca
    15781 ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct
    15841 ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat
    15901 agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc
    15961 ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg
    16021 tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata
    16081 attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca
    16141 ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca
    16201 ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa
    16261 ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct
    16321 ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc
    16381 agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt
    16441 ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa
    16501 aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg
    16561 ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat
    16621 accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc
    16681 tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct
    16741 gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa
    16801 atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac
    16861 tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata
    16921 gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg
    16981 gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg
    17041 gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc
    17101 tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg
    17161 ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt
    17221 tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca
    17281 caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct
    17341 acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca
    17401 cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat
    17461 gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag
    17521 ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca
    17581 tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat
    17641 accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa
    17701 aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt
    17761 tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt
    17821 gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt
    17881 atcaatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat
    17941 ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca
    18001 aacatatcca taataatttt ttctttttca tttcttttat attgttataa cagtgaataa
    18061 cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt
    18121 ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg
    18181 tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat
    18241 atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta
    18301 atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac
    18361 atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt tttgatgact
    18421 tctctaatcc agatgtacct gtcatttttg aaaaaataat acctataata gccaagacaa
    18481 caatacctat aatgacaaac ttcttgttca tatttacttt attcaacttt ttatctccta
    18541 tctccaaaat aatttgttct tatccctctt atcagtacat tgacatccct aaaagtacac
    18601 caaagattgc tccagctacc cctgctatca tcaaaatcgt acccattttc tcatcttttt
    18661 ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta
    18721 acgatactac catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat
    18781 gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg
    18841 ttccggttgt ctcatctgtt tcttgtagtg ctccacaaaa ctggcaatac ttactttcat
    18901 ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat
    18961 tgcttattag ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta
    19021 cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt
    19081 gccacgaact tacacattgc tgtgtaacat tcattttttt tgctaactcc gtttgagaaa
    19141 gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt ctcccctcct
    19201 gttttattat atacaacaat atgatgtatt tcaactcata aaatataaaa aacttcaaat
    19261 accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat taatgaaaca
    19321 gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga
    19381 attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa attctgtaac
    19441 agagcctaac ctcgatagct taacagctct ttctagagcc ctaggaaccc ctgtaagcca
    19501 ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct atcgttctct
    19561 tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa
    19621 tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact ttaacggtgg
    19681 gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt aatacaattg
    19741 ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta aattgataca
    19801 gaggaactat cccagctcct gataatatca attcttttat tgtttcatct atccctgccc
    19861 ctttcgctaa atatattaaa aaactactac ttgctttcac attgtatagt ttcttaatat
    19921 ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg ttttttatta
    19981 aagcattttt cacgctgtta tccccaacat tacgtaggcg ttcacaaaca gtgttaataa
    20041 gttctttctc tgtcattaga tatgtccctt gccacgataa gccctgtaaa acttcatgtg
    20101 cttcatctaa aatcaattgt ttttccttgc tgagttctga tcgggaattt tgaggaaata
    20161 ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa aaatattgat
    20221 atgtaaaatg aattttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
    20281 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
    20341 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
    20401 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
    20461 ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt tcccccgaca
    20521 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
    20581 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
    20641 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
    20701 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
    20761 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
    20821 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
    20881 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
    20941 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
    21001 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
    21061 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
    21121 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
    21181 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
    21241 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
    21301 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
    21361 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg cttaagtatc
    21421 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
    21481 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
    21541 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
    21601 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
    21661 gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt gatgtttcca
    21721 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
    21781 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
    21841 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
    21901 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
    21961 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
    22021 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
    22081 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
    22141 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
    22201 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaacag
    22261 ctttgtaaaa tttatttaca acaccccttt tttgaggtat actaatttta ggaggtgtgg
    22321 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    22381 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    22441 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
    22501 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    22561 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    22621 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    22681 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    22741 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    22801 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    22861 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    22921 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    22981 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    23041 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttaat
    23101 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    23161 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    23221 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    23281 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    23341 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    23401 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    23461 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    23521 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    23581 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    23641 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    23701 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    23761 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    23821 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    23881 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    23941 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    24001 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    24061 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    24121 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    24181 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    24241 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    24301 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
    24361 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
    24421 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
    24481 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
    24541 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
    24601 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
    24661 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
    24721 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
    24781 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
    24841 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
    24901 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
    24961 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
    25021 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
    25081 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
    25141 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
    25201 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
    25261 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
    25321 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
    25381 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
    25441 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
    25501 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
    25561 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
    25621 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
    25681 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
    25741 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
    25801 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
    25861 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
    25921 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
    25981 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
    26041 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
    26101 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
    26161 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
    26221 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
    26281 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
    26341 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
    26401 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
    26461 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
    26521 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
    26581 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
    26641 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
    26701 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
    26761 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
    26821 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
    26881 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
    26941 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
    27001 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
    27061 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
    27121 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
    27181 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
    27241 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
    27301 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
    27361 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
    27421 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
    27481 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
    27541 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
    27601 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
    27661 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
    27721 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
    27781 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
    27841 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
    27901 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
    27961 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
    28021 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
    28081 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
    28141 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
    28201 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
    28261 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
    28321 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
    28381 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
    28441 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
    28501 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
    28561 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
    28621 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
    28681 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
    28741 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
    28801 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
    28861 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
    28921 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
    28981 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
    29041 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
    29101 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
    29161 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
    29221 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
    29281 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
    29341 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
    29401 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
    29461 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
    29521 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
    29581 tatcttttat ttattattaa acaattttga tttctaaatt agaataggta taaaaaagat
    29641 gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct tattttgcat
    29701 cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt
    29761 tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca cctttacgag
    29821 tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca tcttctaatg
    29881 gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca ccttcatcat
    29941 tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag
    30001 ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga
    30061 ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca gaatttgaag
    30121 taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta gagtacaagc
    30181 ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt tctttgattt
    30241 ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct tcttccaaag
    30301 attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt ccttcatctt
    30361 tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt
    30421 tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg
    30481 ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat
    30541 cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat
    30601 tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg tcactgttag
    30661 attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc
    30721 gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa
    30781 tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat
    30841 tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg
    30901 ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat
    30961 agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt
    31021 tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag catacggttt
    31081 gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa
    31141 gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg
    31201 gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct
    31261 caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac
    31321 cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat
    31381 acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg
    31441 catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt
    31501 cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg
    31561 ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc
    31621 ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga
    31681 tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt
    31741 ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat
    31801 ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat
    31861 atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac
    31921 gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc
    31981 ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta
    32041 attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg
    32101 gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga
    32161 cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt
    32221 ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat
    32281 tcacatcttt ctcttatgat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa
    32341 aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca
    32401 gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact
    32461 cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt
    32521 tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt
    32581 ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt
    32641 gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca
    32701 tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct
    32761 tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg
    32821 accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct
    32881 tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca
    32941 acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg
    33001 cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag
    33061 ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg ataagacttg
    33121 gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt
    33181 catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc
    33241 aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac taacgccatg
    33301 ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt
    33361 gtacaatttt cttacctcct tgaagatctt acaggtgctt cattttggta tctcatcgat
    33421 aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg actaatgaaa
    33481 ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca
    33541 tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta
    33601 tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat gacgaatgca
    33661 ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca
    33721 ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc
    33781 aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat
    33841 gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa gtctcttccg
    33901 ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca
    33961 ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa gatcgcaaga
    34021 aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc
    34081 acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc
    34141 gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct
    34201 gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct
    34261 aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa accataaagg
    34321 taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc
    34381 gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc
    34441 ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact
    34501 ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg
    34561 tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg
    34621 gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc
    34681 aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca
    34741 tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc tttcattgta
    34801 tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat
    34861 gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg gaacagagcg
    34921 cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact
    34981 tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg
    35041 aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat
    35101 gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc
    35161 gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgt
    35221 gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg
    35281 caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt cttcattttg
    35341 ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc
    35401 atataataag tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag
    35461 atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa
    35521 aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt
    35581 taataactta aacatggcac cacttccata actttttatg ataattacta taaaaactat
    35641 ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat
    35701 ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct
    35761 attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa
    35821 tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg
    35881 ggttagcttc ccttggaaat ctgtttaaag actatcatca tgttgcttta ggcaatctaa
    35941 ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg
    36001 aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga
    36061 tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca
    36121 aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc
    36181 accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt
    36241 acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt attggaaaaa
    36301 ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg
    36361 tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac
    36421 ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg
    36481 tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac
    36541 gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct
    36601 ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg
    36661 tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc
    36721 ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta
    36781 aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat
    36841 aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct tattaccgca
    36901 gatgcaagct tttgaacacc gtttaccaag aggtcgtaa
//
LOCUS       DACELF010000018        35882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.018, whole genome shotgun sequence.
ACCESSION   DACELF010000018 DACELF010000000
VERSION     DACELF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.018"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW687_000869"
     CDS             <1..1152
                     /locus_tag="IW687_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950151.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW687_000870"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW687_000870"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0950152.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW687_000871"
     CDS             2728..3003
                     /locus_tag="IW687_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0950153.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW687_000872"
     CDS             3009..3776
                     /locus_tag="IW687_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0950154.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW687_000873"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW687_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0950155.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW687_000874"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW687_000874"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0950156.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW687_000875"
     CDS             7920..8222
                     /locus_tag="IW687_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0950157.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW687_000876"
     CDS             8225..9388
                     /locus_tag="IW687_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0950158.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW687_000877"
     CDS             9414..12842
                     /locus_tag="IW687_000877"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0950159.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW687_000878"
     CDS             12863..13987
                     /locus_tag="IW687_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950160.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW687_000879"
     CDS             13977..14414
                     /locus_tag="IW687_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0950161.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW687_000880"
     CDS             14398..14736
                     /locus_tag="IW687_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0950162.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW687_000881"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW687_000881"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0950163.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW687_000882"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW687_000882"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0950164.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW687_000883"
     CDS             15893..16936
                     /locus_tag="IW687_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0950165.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW687_000884"
     CDS             17095..17823
                     /locus_tag="IW687_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0950166.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW687_000885"
     CDS             17939..18184
                     /locus_tag="IW687_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0950167.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW687_000886"
     CDS             18181..18399
                     /locus_tag="IW687_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0950168.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW687_000887"
     CDS             18535..19074
                     /locus_tag="IW687_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0950169.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW687_000888"
     CDS             19067..21340
                     /locus_tag="IW687_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0950170.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW687_000889"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW687_000889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0950171.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW687_000890"
     CDS             complement(22513..22767)
                     /locus_tag="IW687_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0950172.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW687_000891"
     CDS             complement(23167..23436)
                     /locus_tag="IW687_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950173.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW687_000892"
     CDS             complement(23906..24361)
                     /locus_tag="IW687_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0950174.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW687_000893"
     CDS             complement(24499..26427)
                     /locus_tag="IW687_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0950175.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW687_000894"
     CDS             complement(26624..27457)
                     /locus_tag="IW687_000894"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0950176.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW687_000895"
     CDS             complement(27649..28353)
                     /locus_tag="IW687_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0950177.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW687_000896"
     CDS             complement(28376..29002)
                     /locus_tag="IW687_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0950178.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW687_000897"
     CDS             29328..31703
                     /locus_tag="IW687_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0950179.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW687_000898"
     CDS             complement(31727..32218)
                     /locus_tag="IW687_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0950180.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW687_000899"
     CDS             32405..33325
                     /locus_tag="IW687_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0950181.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW687_000900"
     CDS             33479..33823
                     /locus_tag="IW687_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0950182.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW687_000901"
     CDS             complement(34013..34513)
                     /locus_tag="IW687_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0950183.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW687_000902"
     tRNA            35041..35124
                     /locus_tag="IW687_000902"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IW687_000903"
     CDS             complement(35448..>35882)
                     /locus_tag="IW687_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0950184.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6261 c         7406 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcggttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACELF010000019        35657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.019, whole genome shotgun sequence.
ACCESSION   DACELF010000019 DACELF010000000
VERSION     DACELF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.019"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2198)
                     /locus_tag="IW687_000904"
     CDS             complement(<1..2198)
                     /locus_tag="IW687_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950185.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
     gene            complement(2185..2403)
                     /locus_tag="IW687_000905"
     CDS             complement(2185..2403)
                     /locus_tag="IW687_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950186.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2405..3448)
                     /locus_tag="IW687_000906"
     CDS             complement(2405..3448)
                     /locus_tag="IW687_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0950187.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3603..3929)
                     /locus_tag="IW687_000907"
     CDS             complement(3603..3929)
                     /locus_tag="IW687_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950188.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3937..4140)
                     /locus_tag="IW687_000908"
     CDS             complement(3937..4140)
                     /locus_tag="IW687_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950189.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4215..4865
                     /locus_tag="IW687_000909"
     CDS             4215..4865
                     /locus_tag="IW687_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950190.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4920..6086
                     /locus_tag="IW687_000910"
     CDS             4920..6086
                     /locus_tag="IW687_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0950191.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IW687_000911"
     CDS             complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IW687_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0950192.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IW687_000912"
     CDS             complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IW687_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0950193.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7545..8012)
                     /locus_tag="IW687_000913"
     CDS             complement(7545..8012)
                     /locus_tag="IW687_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0950194.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8132..9670)
                     /locus_tag="IW687_000914"
     CDS             complement(8132..9670)
                     /locus_tag="IW687_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0950195.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9750..10436
                     /locus_tag="IW687_000915"
     CDS             9750..10436
                     /locus_tag="IW687_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0950196.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IW687_000916"
     CDS             complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IW687_000916"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0950197.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IW687_000917"
     CDS             complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IW687_000917"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0950198.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11922..13052)
                     /locus_tag="IW687_000918"
     CDS             complement(11922..13052)
                     /locus_tag="IW687_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0950199.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13052..15235)
                     /locus_tag="IW687_000919"
     CDS             complement(13052..15235)
                     /locus_tag="IW687_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0950200.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15232..16257)
                     /locus_tag="IW687_000920"
     CDS             complement(15232..16257)
                     /locus_tag="IW687_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0950201.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16302..17129)
                     /locus_tag="IW687_000921"
     CDS             complement(16302..17129)
                     /locus_tag="IW687_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0950202.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17126..18193)
                     /locus_tag="IW687_000922"
     CDS             complement(17126..18193)
                     /locus_tag="IW687_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0950203.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18219..19055)
                     /locus_tag="IW687_000923"
     CDS             complement(18219..19055)
                     /locus_tag="IW687_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0950204.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19052..19921)
                     /locus_tag="IW687_000924"
     CDS             complement(19052..19921)
                     /locus_tag="IW687_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950205.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19991..21274)
                     /locus_tag="IW687_000925"
     CDS             complement(19991..21274)
                     /locus_tag="IW687_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950206.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21288..22973)
                     /locus_tag="IW687_000926"
     CDS             complement(21288..22973)
                     /locus_tag="IW687_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0950207.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23248..24273
                     /locus_tag="IW687_000927"
     CDS             23248..24273
                     /locus_tag="IW687_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0950208.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IW687_000928"
     CDS             complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IW687_000928"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0950209.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IW687_000929"
     CDS             complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IW687_000929"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0950210.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32945..33913
                     /locus_tag="IW687_000930"
     CDS             32945..33913
                     /locus_tag="IW687_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950211.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33931..34836
                     /locus_tag="IW687_000931"
     CDS             33931..34836
                     /locus_tag="IW687_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950212.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34833..35639
                     /locus_tag="IW687_000932"
     CDS             34833..35639
                     /locus_tag="IW687_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0950213.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10106 a         7531 c         6435 g        11585 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttc ctcacgattc attgcttcag gcgtgtattt tgcttcctgt
      301 cgcttctgat taattaatac ttcagcagac ttagcttgac cgtgcatagc atttttacgt
      361 tcttcaatat cttgtttaac tttttgctga attgcacttg caggattaat atttgattca
      421 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcagcgttt
      481 tcaactttag cgccatcttt tctatccgaa agacgtgttg gcatagaatc agcatatttt
      541 ccagtttcat cttctttgtt tttagctact ataggtttgc tatttttatt aatagcgtac
      601 acggtaccat catctaaacc ttctttgaat ttattcttgg tatcattagc catatcacgc
      661 atactcttaa atggttttgt tgctctttca ctggccattg tagctccatc cgaagctaag
      721 cctgataatc ctcgttcacg agcataccca agtgttttcc ccgttttatt agctttttga
      781 gaaagtgatt gaccaaagtt gcgttcaggt tttcgttctt ccccttccgc ttgatcgttt
      841 ccaccactat ttttcccacc tttagatcgg ttcataccac ctgttccttt acgaaacagc
      901 atagctccca tgccaaatgc tgaagcaagc tggccatagc cttcttttaa accaatatcc
      961 acgccaaagt aacgcatgac tgttccacta ccctttatca gcacaataac agcgcagacg
     1021 tacgctactg cttttataaa aggatttaca gaagaaccgc tacctgttcc gagataatta
     1081 acaaacattg caaacataga caatccgaac ccttgaaatc cgactgtcaa ataacactgt
     1141 aaaatatctg acaaaacttg ctttgatcgt tggcctgttt ccaagtcagt agcaaatact
     1201 aaaactccaa gtacgcgttt aaacgctagc tcgagtattg ctgtaattat aatgaaagca
     1261 ctaaaaacat acgctaccgc taaagcaatc aatccaaaag aaatccccca tagatttgct
     1321 tgatagcgat aatatcctga tttaatgccc tcaacaaagg tcgacagaaa attatccgaa
     1381 aatttggtag caacaaattc attatcagaa ttttcaacta attcataacg cagattatca
     1441 gctattgggt tctctttttc cattttgtcg agtacattat ctgtcaatac ttgcgtaaga
     1501 tccgttttcc aaaaattttc ttttgttaga ttgttccggt cattcgtttt atcgatcatg
     1561 gaatattgat ctgttttatt gatataagct aaatcagtaa ccccttgttc aatcagcgac
     1621 cacgccacgc cttcgtccga atttgtaatc gtagtcgtat cattataaaa tgttttagaa
     1681 aactctattc cgctgtgaat caacgttggc attaaaagaa tcaatgaaat tgacattaca
     1741 acattcattc caacagattt tagatcaacc gttcccttac ctgtaatcat tttataacca
     1801 ataatcacta gtgaaagtat cattaagcca acaacaatgg tacttaaaac agcttgatag
     1861 actttgttca atccggtact ttctacaaag ctaaacaaat ctaaaacttc gggaatcata
     1921 ttttgaatgc catcgacaac agcgaacatt gattttataa cagaccagcc tatccacata
     1981 ccgaactggc tgtaaaaatt tccgttgtat tttttcaaat aattctggta aagcggatta
     2041 gttaaaaact cagtttgact ctcagtatca tcaaaccaat tgaaaatact gccttctgca
     2101 atgttactcg taaaaagaat cacagctatt agaacaagaa aaataaccgg taaccaattt
     2161 gtttttctat tagcaatctt ttttttaatt attttcattg ttacctccct cagcccattt
     2221 tcttaggctc tcagagagtt tctgagctat ttttctgtca attatgatat gagttacgta
     2281 atcatcatta acagctttta aatcgagaac aatagtattt gagcagatac tctcggttaa
     2341 ctcaagagta tctgcgtttt gatcaagaca gtgaatctta tctactagat gattttcaat
     2401 cattttaaat tccctctttt ttttgtagct tgattagttc taacgctttg ttttttagag
     2461 aagtaaagat ataattattt ttttgtttga tacgatgatc tgtttttgct cttcgtatca
     2521 agtcacgtaa agtcattgct agctgttctc taacatgatc tacaaaataa ctattattac
     2581 aaatttcata gataattttc agttgaactt tttgttcttt ttcagcagat ttaattgcat
     2641 aattgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta gccagtttta
     2701 agcaattcac aatactttct ccccacattt gtttaagaag attttcttca ataaatgttt
     2761 cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaaa agatcatcat
     2821 tatgtgattc tttaatatct ttactatctt tataagactt atataattct agttcatttt
     2881 ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg catagttcaa
     2941 attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga actatcaagt
     3001 ttttttgact attttcttga tgggttatct tattaatttt ataaacatca tctttgcaca
     3061 gtacaggctt taacaaataa taaaaatttg ggaacatttg cattttattt ttattgaagc
     3121 cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct gataagattg
     3181 tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc aaaaaaattt
     3241 tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct tttagtaaca
     3301 tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg tttaaaaaaa
     3361 taggatattg atagtataat tctgcatata tatcagaatt agaatagcct tgcttttcaa
     3421 ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt atttgctata
     3481 atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg atctgcaaac
     3541 tgacgggaac actcttttta attttttcta cttcatcatt attcaaaaaa acacctcgtt
     3601 ttttaaattt gatgactcaa tatccatttt ttgcattctt cttttgaaaa caactccatt
     3661 ttttctaata aaataactgg caatcccatt cttttaaatt tcaaaaaagt tgtatatgat
     3721 acattcaaat atttagccat ctcttttcct tttagaaatg gtctttctaa cccggtatca
     3781 attcttgctt gatcaatagc ctcttttgtc atttcaaaaa cataattttt caattgattc
     3841 gtttgttgtt tatctaacat tactgtaatt cctgcatcca ttatttttct cctctcattt
     3901 tctatttctt caatcagcca actcgccaag ttgtatttat ttagatttca aatcattaag
     3961 taaccaattt accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc
     4021 gattttgaca tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag
     4081 tataagatcg gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat
     4141 tcagcgcctc cttttaaatt attcactttg tggtttttat cttaataagc acattttaaa
     4201 ccacgaaacg agtaatgtca attaaaacat tactcaattt gtggttttta tgctataata
     4261 gtttggaatt tactataggg ggtgttactt tgaatagaat aaaattttat attgattcca
     4321 gtaaaaagac cattaaacaa attagtacag aaactggtat taactataca actcttacga
     4381 actattaccg tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt
     4441 atttccatgt tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac
     4501 ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata
     4561 ttgccagtct tgactcagat tattttactg aattatctaa tctatacttt tccatgactt
     4621 ctttacttga agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc
     4681 tagaggactc ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag
     4741 ctttaaactt aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa
     4801 cccaagtaca ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc
     4861 aataaaacta attattcaat cagccaactc gccaagttat cagattggag agattagtta
     4921 tgtcaataca aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc
     4981 gtaaagatga aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag
     5041 aagccaagtt acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc
     5101 cttctaatga cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg
     5161 tacaagaaac gacatatcaa cgtacaaaag accttttccg cctgcatata ctaccaaaat
     5221 tcgggaataa gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat
     5281 gggctttaaa attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt
     5341 atgccatttt tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc
     5401 gggaaaagaa agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata
     5461 ttctcaataa agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg
     5521 gtatacgtaa aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc
     5581 taatgtatgt tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa
     5641 aaactgtcag gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg
     5701 atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc
     5761 agtttggcat aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg
     5821 gtgacattaa tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga
     5881 agaaatataa atttaagcac gttactattc atggattccg gcacactcac gctactcttc
     5941 tactagaagg tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg
     6001 aaactaccct taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa
     6061 aattcattgc ttacactggt ttataatttt gtcattacct ttctcattac cagaacgaaa
     6121 aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg
     6181 ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     6241 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     6301 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     6361 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     6421 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     6481 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     6541 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     6601 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     6661 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     6721 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     6781 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     6841 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     6901 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     6961 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     7021 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     7081 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     7141 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     7201 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     7261 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7321 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     7381 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7441 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     7501 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     7561 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     7621 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     7681 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     7741 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     7801 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     7861 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     7921 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     7981 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     8041 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     8101 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     8161 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     8221 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     8281 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     8341 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     8401 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     8461 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     8521 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     8581 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     8641 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     8701 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     8761 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     8821 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     8881 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     8941 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     9001 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     9061 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     9121 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     9181 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     9241 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     9301 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     9361 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     9421 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     9481 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     9541 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     9601 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     9661 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     9721 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     9781 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     9841 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     9901 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     9961 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
    10021 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
    10081 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
    10141 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
    10201 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
    10261 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
    10321 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
    10381 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
    10441 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
    10501 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
    10561 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
    10621 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
    10681 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
    10741 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
    10801 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
    10861 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
    10921 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
    10981 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
    11041 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
    11101 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
    11161 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
    11221 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
    11281 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
    11341 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
    11401 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
    11461 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
    11521 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
    11581 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
    11641 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
    11701 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
    11761 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
    11821 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
    11881 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
    11941 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
    12001 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
    12061 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
    12121 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
    12181 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
    12241 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
    12301 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
    12361 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
    12421 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
    12481 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
    12541 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
    12601 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
    12661 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
    12721 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
    12781 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
    12841 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
    12901 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
    12961 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
    13021 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
    13081 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
    13141 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
    13201 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
    13261 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
    13321 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
    13381 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
    13441 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
    13501 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
    13561 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
    13621 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
    13681 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
    13741 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
    13801 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
    13861 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
    13921 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
    13981 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
    14041 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
    14101 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
    14161 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
    14221 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
    14281 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
    14341 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
    14401 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
    14461 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
    14521 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
    14581 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
    14641 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
    14701 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
    14761 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    14821 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    14881 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    14941 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    15001 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    15061 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    15121 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    15181 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    15241 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    15301 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    15361 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    15421 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    15481 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    15541 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    15601 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    15661 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    15721 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    15781 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    15841 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    15901 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    15961 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    16021 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    16081 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    16141 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    16201 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    16261 tttctccctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    16321 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    16381 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    16441 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    16501 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    16561 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    16621 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    16681 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    16741 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    16801 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    16861 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    16921 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    16981 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    17041 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    17101 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    17161 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    17221 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    17281 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    17341 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    17401 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatccctc
    17461 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    17521 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    17581 aatcggcacc gtatttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    17641 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    17701 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    17761 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    17821 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    17881 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    17941 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    18001 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    18061 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    18121 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    18181 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    18241 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    18301 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    18361 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    18421 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    18481 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    18541 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    18601 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    18661 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    18721 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    18781 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    18841 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    18901 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    18961 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    19021 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    19081 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    19141 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    19201 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    19261 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    19321 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    19381 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    19441 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    19501 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    19561 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    19621 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    19681 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    19741 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    19801 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    19861 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    19921 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    19981 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    20041 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    20101 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    20161 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    20221 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    20281 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    20341 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    20401 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    20461 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    20521 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    20581 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    20641 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    20701 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    20761 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    20821 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    20881 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    20941 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    21001 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    21061 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    21121 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    21181 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    21241 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    21301 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    21361 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    21421 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    21481 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    21541 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    21601 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    21661 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    21721 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    21781 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    21841 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    21901 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    21961 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    22021 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    22081 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    22141 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    22201 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    22261 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    22321 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    22381 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    22441 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    22501 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    22561 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    22621 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    22681 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    22741 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    22801 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    22861 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    22921 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    22981 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    23041 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    23101 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    23161 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    23221 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    23281 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    23341 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    23401 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    23461 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    23521 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    23581 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    23641 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    23701 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    23761 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    23821 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    23881 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    23941 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    24001 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    24061 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    24121 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    24181 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    24241 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    24301 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    24361 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    24421 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    24481 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    24541 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    24601 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    24661 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    24721 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    24781 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    24841 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    24901 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    24961 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    25021 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    25081 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    25141 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    25201 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    25261 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    25321 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    25381 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    25441 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    25501 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    25561 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    25621 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    25681 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    25741 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    25801 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    25861 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    25921 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    25981 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    26041 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    26101 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    26161 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    26221 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    26281 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    26341 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    26401 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    26461 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    26521 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    26581 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    26641 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    26701 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    26761 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    26821 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    26881 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    26941 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    27001 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    27061 tgatttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    27121 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    27181 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    27241 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    27301 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    27361 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    27421 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    27481 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    27541 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    27601 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    27661 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    27721 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    27781 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    27841 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    27901 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    27961 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    28021 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    28081 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    28141 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    28201 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    28261 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    28321 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    28381 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    28441 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    28501 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    28561 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    28621 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    28681 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    28741 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    28801 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    28861 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    28921 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    28981 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    29041 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    29101 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    29161 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    29221 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    29281 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    29341 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    29401 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    29461 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    29521 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    29581 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    29641 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    29701 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    29761 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    29821 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    29881 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    29941 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    30001 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    30061 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    30121 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    30181 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    30241 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    30301 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    30361 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    30421 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    30481 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    30541 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    30601 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    30661 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    30721 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    30781 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    30841 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    30901 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    30961 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    31021 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    31081 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    31141 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    31201 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    31261 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    31321 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    31381 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    31441 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    31501 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    31561 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    31621 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    31681 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    31741 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    31801 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    31861 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    31921 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    31981 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    32041 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    32101 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    32161 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    32221 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    32281 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    32341 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    32401 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    32461 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    32521 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    32581 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    32641 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    32701 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    32761 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    32821 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    32881 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    32941 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    33001 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    33061 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    33121 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    33181 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    33241 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    33301 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    33361 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    33421 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    33481 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    33541 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    33601 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    33661 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    33721 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    33781 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    33841 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    33901 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    33961 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    34021 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    34081 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    34141 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    34201 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    34261 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    34321 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    34381 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    34441 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    34501 tctaatggat gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat
    34561 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    34621 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    34681 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    34741 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    34801 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    34861 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    34921 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    34981 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    35041 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    35101 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    35161 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    35221 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    35281 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    35341 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    35401 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    35461 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    35521 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    35581 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    35641 cagaaagagg atgggac
//
LOCUS       DACELF010000020        34685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.020, whole genome shotgun sequence.
ACCESSION   DACELF010000020 DACELF010000000
VERSION     DACELF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.020"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(233..1081)
                     /locus_tag="IW687_000933"
     CDS             complement(233..1081)
                     /locus_tag="IW687_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0950214.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IW687_000934"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IW687_000934"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0950215.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IW687_000935"
     CDS             complement(3582..3761)
                     /locus_tag="IW687_000935"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0950216.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IW687_000936"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IW687_000936"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0950217.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IW687_000937"
     CDS             5909..8188
                     /locus_tag="IW687_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0950218.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IW687_000938"
     CDS             complement(8281..8928)
                     /locus_tag="IW687_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950219.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IW687_000939"
     CDS             complement(9107..9826)
                     /locus_tag="IW687_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0950220.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IW687_000940"
     CDS             complement(9957..11108)
                     /locus_tag="IW687_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0950221.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IW687_000941"
     CDS             complement(11325..12065)
                     /locus_tag="IW687_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0950222.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IW687_000942"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IW687_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0950223.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IW687_000943"
     CDS             complement(12449..13042)
                     /locus_tag="IW687_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0950224.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IW687_000944"
     CDS             complement(13026..13673)
                     /locus_tag="IW687_000944"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0950225.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IW687_000945"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IW687_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0950226.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IW687_000946"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IW687_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0950227.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IW687_000947"
     CDS             complement(15119..15646)
                     /locus_tag="IW687_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0950228.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IW687_000948"
     CDS             complement(15826..16611)
                     /locus_tag="IW687_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0950229.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IW687_000949"
     CDS             complement(16604..17473)
                     /locus_tag="IW687_000949"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0950230.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IW687_000950"
     CDS             complement(17474..18853)
                     /locus_tag="IW687_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0950231.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IW687_000951"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IW687_000951"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0950232.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IW687_000952"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IW687_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0950233.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IW687_000953"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IW687_000953"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0950234.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IW687_000954"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IW687_000954"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0950235.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IW687_000955"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IW687_000955"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0950236.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IW687_000956"
     CDS             complement(22811..23035)
                     /locus_tag="IW687_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0950237.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IW687_000957"
     CDS             complement(23088..24050)
                     /locus_tag="IW687_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0950238.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IW687_000958"
     CDS             complement(24060..24500)
                     /locus_tag="IW687_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0950239.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IW687_000959"
     CDS             24806..25771
                     /locus_tag="IW687_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0950240.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IW687_000960"
     CDS             complement(25828..26058)
                     /locus_tag="IW687_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0950241.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IW687_000961"
     CDS             complement(26144..27031)
                     /locus_tag="IW687_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0950242.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IW687_000962"
     CDS             complement(27120..28343)
                     /locus_tag="IW687_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0950243.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IW687_000963"
     CDS             complement(28360..29439)
                     /locus_tag="IW687_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0950244.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IW687_000964"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IW687_000964"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0950245.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IW687_000965"
     CDS             complement(31297..31563)
                     /locus_tag="IW687_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0950246.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IW687_000966"
     CDS             32042..34276
                     /locus_tag="IW687_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0950247.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..>34685
                     /locus_tag="IW687_000967"
     CDS             34424..>34685
                     /locus_tag="IW687_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0950248.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIEL"
BASE COUNT         9804 a         7492 c         6041 g        11348 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attgg
//
LOCUS       DACELF010000021        33711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.021, whole genome shotgun sequence.
ACCESSION   DACELF010000021 DACELF010000000
VERSION     DACELF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.021"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            3..815
                     /locus_tag="IW687_000968"
     CDS             3..815
                     /locus_tag="IW687_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0950249.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IW687_000969"
     CDS             812..1612
                     /locus_tag="IW687_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0950250.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IW687_000970"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IW687_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0950251.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IW687_000971"
     CDS             3109..4818
                     /locus_tag="IW687_000971"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0950252.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="IW687_000972"
     CDS             5030..9382
                     /locus_tag="IW687_000972"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0950253.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="IW687_000973"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="IW687_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0950254.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="IW687_000974"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="IW687_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0950255.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="IW687_000975"
     CDS             11220..11513
                     /locus_tag="IW687_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0950256.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="IW687_000976"
     CDS             11510..11821
                     /locus_tag="IW687_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0950257.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="IW687_000977"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="IW687_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0950258.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="IW687_000978"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="IW687_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0950259.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="IW687_000979"
     CDS             complement(14622..14843)
                     /locus_tag="IW687_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950260.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17653
                     /locus_tag="IW687_000980"
                     /pseudo
     CDS             15291..17653
                     /locus_tag="IW687_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18021..18944
                     /gene="truB"
                     /locus_tag="IW687_000981"
     CDS             18021..18944
                     /gene="truB"
                     /locus_tag="IW687_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0950261.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18948..19889
                     /gene="ribF"
                     /locus_tag="IW687_000982"
     CDS             18948..19889
                     /gene="ribF"
                     /locus_tag="IW687_000982"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0950262.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20241..21026
                     /locus_tag="IW687_000983"
     CDS             20241..21026
                     /locus_tag="IW687_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0950263.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21039..21833
                     /locus_tag="IW687_000984"
     CDS             21039..21833
                     /locus_tag="IW687_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0950264.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22013..22465)
                     /locus_tag="IW687_000985"
     CDS             complement(22013..22465)
                     /locus_tag="IW687_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950265.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22930..23298)
                     /locus_tag="IW687_000986"
     CDS             complement(22930..23298)
                     /locus_tag="IW687_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0950266.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23448..23930
                     /locus_tag="IW687_000987"
     CDS             23448..23930
                     /locus_tag="IW687_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0950267.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23967..24977
                     /locus_tag="IW687_000988"
     CDS             23967..24977
                     /locus_tag="IW687_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0950268.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25092..25805)
                     /locus_tag="IW687_000989"
     CDS             complement(25092..25805)
                     /locus_tag="IW687_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950269.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25995..26585)
                     /locus_tag="IW687_000990"
     CDS             complement(25995..26585)
                     /locus_tag="IW687_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0950270.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27069..27296
                     /locus_tag="IW687_000991"
     CDS             27069..27296
                     /locus_tag="IW687_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0950271.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27312..28478
                     /locus_tag="IW687_000992"
     CDS             27312..28478
                     /locus_tag="IW687_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0950272.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28712..29755
                     /gene="hrcA"
                     /locus_tag="IW687_000993"
     CDS             28712..29755
                     /gene="hrcA"
                     /locus_tag="IW687_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0950273.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29849..30412
                     /gene="grpE"
                     /locus_tag="IW687_000994"
     CDS             29849..30412
                     /gene="grpE"
                     /locus_tag="IW687_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0950274.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30463..32292
                     /gene="dnaK"
                     /locus_tag="IW687_000995"
     CDS             30463..32292
                     /gene="dnaK"
                     /locus_tag="IW687_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0950275.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32443..33609
                     /gene="dnaJ"
                     /locus_tag="IW687_000996"
     CDS             32443..33609
                     /gene="dnaJ"
                     /locus_tag="IW687_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0950276.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11458 a         5813 c         7275 g         9165 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatcctgt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgca ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaattcaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta
    16741 cgaatcattc ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa
    16801 atcacatgac ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac
    16861 agtcttccag caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt
    16921 ggcagaaaag aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct
    16981 cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa
    17041 acatccgcgt gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt
    17101 gaaaatcatt gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc
    17161 tgcaggaacg gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt
    17221 cccaaaacta aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag
    17281 tcaagatccg cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc
    17341 gatcttattt gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca
    17401 taaccatcat ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga
    17461 catgcgtgta gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat
    17521 caaaggaagt caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca
    17581 aaactatatt cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa
    17641 tcttgaccaa taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag
    17701 aaactgaaaa aataaataaa aaaaacggaa aaaagactgg aagaatggcc agtctttttt
    17761 ttcgtttttg ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca
    17821 aatggtgcaa agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt
    17881 tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca
    17941 ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa
    18001 aaatgaaatg gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga
    18061 caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg
    18121 ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa
    18181 aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt
    18241 tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt
    18301 ctttatctga aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga
    18361 taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca
    18421 ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa
    18481 gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg
    18541 ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg
    18601 cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga
    18661 cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa
    18721 tagaaaaagg agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag
    18781 taaaaaacgg tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag
    18841 aaattgcttt gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa
    18901 aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca
    18961 agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg
    19021 gatttttcga cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg
    19081 cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct
    19141 ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa
    19201 aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc
    19261 atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg
    19321 tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg
    19381 aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa
    19441 aaatcagctc tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa
    19501 aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca
    19561 gactactagg attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag
    19621 aaggtgttta tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa
    19681 ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg
    19741 actttaatca agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag
    19801 atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag
    19861 caacagctga atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta
    19921 taactagcaa ttacaataaa atgggtgata aataagccca ggaaaggggc tgtgacaaaa
    19981 gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt cttcggaaat
    20041 aagccgaaat ttcttgaagc tgaccacttc tgtcacaacc tctttcttta tagaaataaa
    20101 aggacactag caattcatga aaacgattgc tagagcgtat catttgcttt agactatata
    20161 tcttggtgta cactcacttt aaggcggaaa taaaagttat ccgtaataac aggtttaggt
    20221 atgaaaaagg agtgggtact atgtacaagg aattagaaaa caaagtcgca gtagtaactg
    20281 gtggctcaaa ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg
    20341 tagttgtcaa ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag
    20401 aggcaggcgg aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc
    20461 gtttacttga tacggcttta gaaaattttg ggacactgga cgtttgggtt aataatgctg
    20521 ggatggaaaa tcagcatcca acccatgaac ttagccttga agattgggaa agagtgttaa
    20581 aagtcaattt gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga
    20641 ataataaaaa aggaaatatc atcaatatgt cttctgttca tgaacagatc ccatggccaa
    20701 cgtttgctca ttacgctgct tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa
    20761 tggagtatgc accgaaggga atcagaatca atagtatagg ccctggtgct atccgtacac
    20821 ctattaatgc ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc
    20881 cactaggacg cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag
    20941 acgaatccag ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc
    21001 cttctttcca aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga
    21061 tcccggcagt tggctggggg atccagccac tgattttgaa aaagattggt ggcagaccaa
    21121 caaatgagat attaggaact ggtattggag cggtgcttgt cggcttactc gttcaattat
    21181 tcgcttctcc agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat
    21241 tttgggttat tggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa
    21301 cgatgccaat ttcgactgca ttacagttag tcggaacctc gttgattggt gtcttcgctt
    21361 ttggagaatg gtctggaagt atggggaaaa tcattggagc agcagcaatc attcttcttg
    21421 tatttggttc tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa
    21481 aaggaattag tctattagca tcaacaagta ttggttattg ggtatacagt gcattgccta
    21541 aactagttga tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag
    21601 gggcagtgat ttatgtgatt ttccgtaaac caaccgcatt ttctgaagac aagagttgga
    21661 aagcaacgat cgttggagcc atctttagtg tgtcagcatt ggcttatatt ttttcagcaa
    21721 atgaaaatgg ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat
    21781 taggcggtat gtacatcctg catgaaaata agacaccaaa agaaaaaaat taacgattat
    21841 cggattattg ctgatcgtag taggaagcat ggtcacggtc tttatttgag gataaagatg
    21901 gtaaaatttc atttattaaa aaaatatttg tttgaaccaa acagtaagta cttataaaaa
    21961 aatagcgcta gaatttccaa catctctgga atttctagcg ctttttaatt gtttaagata
    22021 caagggaaat atctgtctgg atcaataatt tttcattgat ccattgacta aagtttacga
    22081 cataattgaa gttaccaccg attagtatct ccgcatgatt gtctttataa agatcgtatt
    22141 gtaaaagacg gtctttttta tttgcgtaaa catcgttatg gtactggact aattgttctc
    22201 ggaattgatt gattgctgta aaatcacgat attgaaggtt gaaacgcatc gtgattggtg
    22261 catttagcat gaatcgctgc atttcttttg ctgcttcctc tgcttgatct ccgtatagga
    22321 actcaaaaag attacgttca gaaaatttcc attgcccgcc aacctttgtt ccttttagtt
    22381 ttccttcatg aagataattt ctgatagaac gtgtagtaac acctaattgt tcggctaatt
    22441 catttactga gtaaaaatct ttcaatgttc accctcctat gtgaatggtt ctactgactt
    22501 agttgatatt tgcttaatca cgaaatgtag aaagaacgat tttaattaag gtcattgtaa
    22561 aaatcaagtc agaaaattct aacttttatt tctctaaaga aattcgttct ctcctaaagt
    22621 gatattatat ttaaatatac ctgaaaataa tgggcagtat tatgaaagat cactgatatt
    22681 ttctcgtatt ttttccttat tcaaaagttt tgcaggaaaa ttgctgaaaa aataatttta
    22741 ctaagtagaa aattcaaagc cattcaatca gtgatttttt tcagcacaag aaagactcct
    22801 tataaaaata gcagatactt tgtcaattaa gtgtttaacc agggatgata aaaaggatag
    22861 gattgaacaa aacctgtata aggtgatgaa tagtttcctc gccagtaaaa ggtttattcg
    22921 tataaagctt tatcgccaaa atttatcatg acctttttta tcttcaatat taaatgaaat
    22981 caattgttca atcaattccc agttgattac acttttccaa ggaactttga tgacgttatc
    23041 cgtatatacg tagcctgctt cttggatatt tttttcaagg gcacgaatcg ctgctttttc
    23101 cggtgcgata gaaaaatgat ttttagcaaa actaaatgca ataataaagg taccatggtc
    23161 agtaaacata ggttggttcc attttacagt tgtgtctagc tggggatacg tttctgttat
    23221 cttagcaaaa aggtctttga tcttttgttg atgttctttt tctttgattt gattgatgat
    23281 cggctgaaat ttttccattc taaaacgcct ccattaagtg gtttgattta ttttaccaga
    23341 aaaacaatag ttatgtataa aaaacgaaca aaacacaagt aatgttgtaa ccgctatatt
    23401 tatatgataa gatgtctcaa ataataaaat agaaaagaga cataaaaatg attgacttgg
    23461 caaagaaatt cttacacgaa aatatatatt ttgagttgta tcatcatcga gccatttata
    23521 caaatgaaga tgctttggta ataaagaaag aacaaggatt tacaggtaca gagaccaaaa
    23581 gcttattctt aaaagataaa aaagataata actacgtata tctgactttt actacgaaac
    23641 aaacggattt caaagcatta gggaagatcg tcggtaaaag gctttctatt gtttcatcag
    23701 aacgcatgga agaacaaacg ggacaaaagc agggcgctgt ttctccgttt ggttatgaaa
    23761 aacagattcc gattattatt gatgaagagc tcttatcaaa tgaaaaactt gtttttgcac
    23821 caggtagacc agatcaaacg atggtcatcc aaacggttga cttaaacaaa atcatccagc
    23881 tacttgagat gccgatattt ttgatgccgg caaacgaaaa aaacaattaa ccaagtaaaa
    23941 tagtgcatac ttggaggtga agcaacatga taagttatcc attattagaa agaaatgcaa
    24001 cgattggggt tactgcacca tcctcaggcg tatcagagga acaccattcg ttactcgaat
    24061 cagctttaaa gagaatggaa gaaaaatctt tccaagtagt tactggaaag acgctttgga
    24121 cgcaagataa ggaaaaatca ggtccagcaa gaaaacgagc agatgaattc aatcaaatga
    24181 tgcaagatga aaatatccaa atgatctttc cgacttgggg aggagaatta ttgattgaga
    24241 ttctcgattt tgtcgatttt gatgctctac aaccaaaatg gatagtaggt tattcagata
    24301 ttagtacgtt gctacttgct gtcacattaa aaacagggat agccactgca catggaacta
    24361 accttattga tttaagagga gagtacttgg atgaaacgac agcaatgtgg gaaagtgtac
    24421 tgaaaacaag agaaggagaa tcgattctac agcaatcttc tgaaaagtat caaaaagtgt
    24481 ggccccatga gcattcatca tcttctgttt tccatctcac tgaaccaaca aattggcaat
    24541 cagtcagcgg agatgcagta aggatgaagg gacgattatt aggtggttgt attgatacga
    24601 tcaggcattt aatcggtact ccttatggag atgtccaatc ttttaaaaga aagtataatg
    24661 atggaaagcc gatcttgtgg tttctagaaa actgtgagct atcgtcagtt gatttaagaa
    24721 gatcacttgt tcagatgaaa ctggcaggct ggtttgatgg cagctcgggg atcatgttcg
    24781 gtagaagtgc ggcagaggat ttgttagatt ataaaatgaa ggatgtatac gaagatctgt
    24841 caaaggaatt aaaactaccg atcttgtatg atattgattg tggtcatgta ccgccacaac
    24901 tgacattgat taatggtgcc tatgcagaag tagcggtaga agatagaaaa gcgactgttt
    24961 tgcaaacgtt tatttaaaaa attttttcct gccttggagt ctatcaaaga ccaataaatc
    25021 taaatagaaa agaaagtgaa agaagctgac aaaatgctgt cagcttcttt ttgtataaca
    25081 tattagaaag tctaattatc tataactgtc acatcgatta actgtttcac gtcattggga
    25141 tttcctgtag gaatagctac tacagatgca ttatcaatga tttcttgagt tgtttctcta
    25201 atgaacgctg gataagatgt ttgatctgca ggcatttgcc aatgtgaagg aactggataa
    25261 ctagtgattg tcccgtcatg gttacttgaa tacactaaaa ctccctggta actgtatttc
    25321 ccagtgagca taaccacatc tttcggatag actgcactcc ctgcatcata aggggcgata
    25381 ggtgttcctg caggataatg aataacatgt aattcgaaat cacccgatgt atctctaaaa
    25441 gtattacctc taatagctag ccaaacacgt gcgtactcaa tttgtagatc agaatatcct
    25501 gctaacggat ctattgactg agaagactgt gaggaggttg tgtctattgt tgaagaagaa
    25561 atatttgtct caactgtact actagatgta gaagaagtat tgggctctga gactttactg
    25621 cttgttgaag atgtattcgt ttcagtagaa gctgatttct gagtacttga tttttcagct
    25681 gaactggttt gtctgattga acttttgtct gtggtcgcag atggatcact attttcttta
    25741 ctaggagacg tacatcctac aaggaataaa gagaccaaca ataaaataaa acctaacttt
    25801 ttcatataaa atacctcatt atttttttaa taccagtata gcataatcct gaatttttga
    25861 atccgtaaaa ccttgtctat gaatataaat agagtgtatc ttctttgata aatgtcagaa
    25921 aaagtgtgac aaataatcat cagtaaaatt acttgtcaca ctattgataa ggagtattta
    25981 attgaagaga atgattagga gaaaggatct ccgtactttt tatagtaatc atagaatcca
    26041 agtttgtata gacgaatcgt tttttctctt atttcttctg tttcaaaatg gactgcttga
    26101 agaatcaagt ctgtgaattc tataggagct gttccattcg cagtaatcaa attacgatct
    26161 ttcacagctt gttgttctat aaaatcttct ttattctgat aattttccga ttctaaccaa
    26221 ggaaattgcg catttccggt gtgtttgtaa ccttgcaata atccgctctg agctaaataa
    26281 tcaactgccc cacaaatagc agctactggt acgtctgttt ttaacaattt atcgattatt
    26341 tgatataaat gatgattttt tatgtcccat gaattgccac cgatcataat aaaaaggtca
    26401 actttttcag gtatttgttc gattaagtaa tcgatttttg tttttaatcc tccaatggaa
    26461 gtgacctctg attggataga agcagttttg ttttgccatt tctcattttg gtttagtgtg
    26521 ctagtaatat aagcaccttc ccaatcggca tattcctcta atagaaacgt aatcgcagtt
    26581 ttcattttca agctctcctt tactatctat aacaaggaca acagttttaa cctacagaca
    26641 ttcattaaaa ttgggtagcg acagcgaaaa caacaattct taagttttag acagagtata
    26701 cagatagtca acttaattaa atacgaacta aataaaaatg acaggtaatc atttttgttt
    26761 accagcatta taacattttg catacatgga tgacaaaaga accaattccc acttgcttga
    26821 gtacaatatt aaaagaaact ggaaggcaaa aagtaattat agaaacgaaa aataaatcat
    26881 atcgggcact cagacatgaa aacaacccta gagatatata actaggtaac cagtttaatg
    26941 aaagaaaaaa tcatccaaga agtcgattct tggatttttt agtggatttg ttcgccaaat
    27001 acgtttagta tgcgtatatg agaggttgta agcaaagcgt tgtggtctga gcacttaagg
    27061 aggaatctgt gaaaatagct tttcatattt ttgcagattt tgacttaatg ccgaaggtca
    27121 gctttgcgat taccgtttat cgagaggctg taaaagcacc gtgtagctcc gatgacaata
    27181 agcaagcatt tttagagcta ggtttccttg attctaaata tgcggcttat ttcaatgagt
    27241 tggtgcttga acgcgatttt aagaaggtcg aaaacaggca cgacttttcg acgtaaggag
    27301 aaagaatttc aatgatagaa acaataaatc ccgcagaaaa caaggtttct gcgtacattc
    27361 atatcccatt ttgcgagcat atctgctatt actgtgattt taataaagtt tttttagaag
    27421 ggcagccagt agatgagtat gtggaaatgc tgctgaatga aatgaaaata atgctagaaa
    27481 gtcatccgac agcgaattta gaaacacttt acgtaggggg cgggacacca acttctttat
    27541 ctgctaaaca acttgatcga ctgttatcag gtgctagaga gatcctgcct tttcaaaatg
    27601 gtaatgagtt tactgtagaa gcgaatccag gtgatttgac ccgtgaaaaa cttcaagtaa
    27661 tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca gacatttgat aatcgcttat
    27721 tgaaaaaaat tggacgtaaa cacaccgcag aagatgtgta tcaaactatg caatttttag
    27781 aagcagaaaa ttttaccaat gtaagcatcg atttgattta tgcattaccg ggacaaacac
    27841 tagaaggata tctggatact ttggaaaaag ctttggcttt ggatctgcct cactattctt
    27901 tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa ctgggtacga caaggaagac
    27961 tcgaactgcc tgatcaggaa acggaaacca gaatgtttga agagacgatt gaagcaatgg
    28021 aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg gctctccgga catgaatctc
    28081 aacataacct catgtactgg aacaatgacc actattttgg gtttggagct ggtgctagcg
    28141 gttatcttgg agatattcgt taccgcaaca aaggaccaat ccaacactat ttacgcccat
    28201 tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt gacacgaaaa aatcaaatag
    28261 aagaagaaat gttcttagga ctaagaaaaa aaacagggat tactaaaaaa catttctatg
    28321 atcgctatca aaaaacaatt gaatcggtgt acggtgatgt gctaagggaa ttagagcaag
    28381 aagggttact tgttaatgaa gaagaccgtg tttatttgac accaagaggg acctttttgg
    28441 ggaacaatgt gtttgagcgc tttttgtttg aaaaatagtt taaagaatat ttgtttttta
    28501 tctgaattta aataaaaaaa gttcaaaaat acaataaagg actattgaaa cgttgatata
    28561 actggattta catcaattat ttagaattag cactcttgtg tttagagtgc taaaaaatgg
    28621 agtctttctc ttgacaggag aagggcttct tgctatattt ataagtggat tagcacttag
    28681 aatatatgag tgctaatgag aggtgaggaa gatgttaaca aagcgacaaa acgacatttt
    28741 acgtctgatc atccaacact atacgagtag tggcgtgccc gttggttcca aaacattgat
    28801 gtctgaggga gtcaaggcca gctcagcaac gattcgcaat gatatgaaag cattagaaga
    28861 tgaaggattg ctgttgaaga ctcactcttc ttctgggcgg attccatctg ctttaggcta
    28921 tcgttattac gtggatcatt tgttaaaacc tgctcgcgta gcagaagatg atttgctgct
    28981 gattcgtcag tctctaggca gagaatttca tgaaatcaat gagattatta agcaatctgc
    29041 tgagatatta tctgagctga caagctatac gactttttca ttgggacctg aaataaaaga
    29101 ccgccgactg actggctttc gtatcgtacc cttgaataat cgacagatta ttgctatcgt
    29161 cgtaacagat atggggaatg tagaaagcca agtgttcact cttcccgaat atatgggaag
    29221 tcaggaacta gaaaaaatgg tacagattgt aaacgaccgt ctagtcggag atcctctgat
    29281 gacggtgtac cataaacttc gtacggagat tccgatgatt cttcataaat atttccaaac
    29341 gacagaaggg atgtctcagt tatttgacac cgttcttggt catgtttttg aagataaggt
    29401 ttatgtcagt ggacaaatga atctcttgga ttatgagccc cgtcaagatc ttgatcagtt
    29461 caaatcgatg ttttctttta tgaaagactc agacggactt acacaaatga tcactccgat
    29521 ggatagcaat attcatatca agattggtgc tgaattaggc aatgatttat tgcagaatat
    29581 gagtatgatt caagcaagtt acgagataga tggtcatgga agaggtacaa tagccttgtt
    29641 agggcctact agtatgcctt attctcggat gttcggtcta gtcgatgttt atcgaagaga
    29701 actagcagct aaacttgctg attactaccg ttcgcttgac ttgtcaaact cgtaatgtga
    29761 ataacgaagc tatttgcagc tgatcgttct ctctttctta gtaagagcaa tcaaacagct
    29821 gactgaataa tgaaaggaag actgtatcgt gaaagaaaat caagaagaac tagataaaga
    29881 aatgacagat gctcagccag aaccggaaat cgatgtcgaa gctgcagaag attcgggtat
    29941 ttcagaagct gaagcggaag aattcgaaac agcgaaatta aaagctgagt tagaagaaat
    30001 ggaagataaa tacttgcggg ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa
    30061 tgagcgtgag caattacaaa aataccgttc acaagattta gccaaaaaat tacttccatc
    30121 aattgacaac ctggaacgtg cattagccac agaagtgtca gatgatcaag gggcaggttt
    30181 gaaaaaaggc gtggaaatgg tcttggaaag tttgcgtaac gcactggaag aagaaggaat
    30241 tgaaaaaatc cctgctaaag gagaagcctt tgatcctaat cttcatcaag cagtacaaac
    30301 tgttccagcg actgaagata caccagccga tacgattgta gaagtactac aagaaggcta
    30361 caaattacat gatcgcgtgt taagaccaac catggtcatt gttgcacaat aattgtgcgg
    30421 atataaaact tacactttca attaaagaag gagacgttaa ttatgagtaa aattatcggt
    30481 attgacttag gtacgactaa ctctgcagta gcagtattgg aaggaaacga agcaaaaatc
    30541 atcgctaacc cagaaggaaa ccgtacaaca ccttctgttg tatcattcaa aaatggagaa
    30601 atccaagtag gggaagttgc aaaacgtcaa gctgtgacaa acccaaatac gatttcatca
    30661 atcaaacgcc atatcggtga agcaggttac aaagtagatg tagaaggtaa atcatataca
    30721 ccacaagaaa tctcagcaat gatccttcaa tatatcaaag gattcgcaga agactattta
    30781 ggtgaaaaag tcgaaaaagc agttatcact gtgccagctt actttaatga tgcacaacgt
    30841 caagcaacaa aagatgctgg taagatcgct ggtctagaag tagaacgtat cgtcaatgaa
    30901 ccaactgcag ctgctcttgc ctatggttta gataaaacag atcgcgacga aaaaatctta
    30961 gtattcgacc ttggtggcgg tacttttgac gtatctatcc ttgaactagg agacggtgtc
    31021 tttgatgtat tgtcaacagc tggcgacaac catctaggtg gggacgactt tgataacaaa
    31081 atcatcgact acatggtagc agaattcaaa aaagaaaaca gcattgactt gtctcaagac
    31141 aaaatggctt tacaacgctt gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc
    31201 gtatcaagca cacaaatcag cttgccattt atcacagcag gagaatctgg accattgcat
    31261 ttggaaatga cattgacacg tgcaaaattt gatgaattaa cagctgactt agtagaacgt
    31321 acaaaagtac cagtacgtca agcgctaaaa gatgcaggtt tatcacaatc agaaatcgac
    31381 gaagttattt tagtcggtgg atctactcgt atccctgcag ttgtagaagc agtacgtaaa
    31441 gaaacaggaa aagaaccaaa caaatcagtt aaccctgatg aagtagttgc tatgggtgct
    31501 gctatccaag gtggcgtgat cactggtgat gtgaaagatg ttgttttact tgacgttaca
    31561 ccattgtcat taggtatcga aacaatgggt ggagtgttca ctaaattgat tgaccgcaat
    31621 acaacgattc caacaagtaa atcacaagta ttctcaactg cggcagataa tcaaccagca
    31681 gtagacatcc atgtattaca aggtgaacgc ccaatggctg ctgacaacaa aactctagga
    31741 agattccaat tgacagatat tccagcagct cctcgtggta ttccacaaat cgaagtaaca
    31801 ttcgatattg ataaaaacgg gattgtaaat gtatctgcga aagatctagg tacacaaaaa
    31861 gaacaaaaaa tcacgatcaa atcttcttca ggtctaacag acgaagaaat cgaacgtatg
    31921 gtaaaagatg cagaagcaaa tgcagaagca gataaagctc gtaaagagga agttgaccta
    31981 cgtaatgatg tagatgcatt gctattctct gtggacaaaa cattgaaaga attagaaggc
    32041 aaagtggacg aagaagaagt gaaaaaagca gaaacagctc gcgatgaatt gaaagccgct
    32101 gttgaagcaa acaacatcga agaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt
    32161 caaaacttga ctgttaaact atacgaacaa gcggcacaac aacaagctca agaaaaccca
    32221 gaagcagctc aaggcggagc agatgatgtc gttgatgctg attttgaaga agtcgatggc
    32281 gacgataaat aatcagttca ctgaatttcg agagaaggcc aaagttgcta ctttggcttt
    32341 ttcttttgaa aaatagtttg aagtatcggt ttttgtttca aacaaaatat gatatgataa
    32401 caagcgacgt gaaaatgatg agaaccaata ggaggaaagc ctatggcaac aaaacgtgat
    32461 tattatgaag tcttagggct ctcaaaagga gcatcagaag atgaaataaa gaaagcttat
    32521 cgtaagcttt cgaaaaaata ccatcctgac atcaataaag aacctgatgc ggaagaaaaa
    32581 ttcaaagaag tatcagaagc atatgagatt ttaagtgacc cgcaaaaacg cgctgcatat
    32641 gaccaatacg ggcatgcagg cacagatcca aattacggtg ctggtggagc tggtggcgga
    32701 tttggtggat tcggcggctt ctctggtggc ggcttcggtg gttttgaaga tatcttcgac
    32761 tctttctttg gaggaggcgg tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat
    32821 ttgcaatata cggtcgactt gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc
    32881 aagtataatc gggaagaaat ctgtcatact tgtggtggga acggtgcaaa accaggcaca
    32941 caaccaacta cttgtcataa atgtcatggt gcaggtacga ttaatgtaga acgccaaaca
    33001 ccacttggac gagtgatgag ccgtcaaact tgtgatgttt gtcatgggac agggaaagaa
    33061 atcaaagaac cttgtccaac ctgtcatggc tcaggacatg agaagaaagc acattcagtt
    33121 aaagtgaatg taccagctgg tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga
    33181 gaagctggag aaaacggcgg accatttggt gatttgtatg tggtcttccg tgtagaagaa
    33241 agtgatatct ttgatcgtga aggttctgaa atttactatg aattgccttt aagttttgta
    33301 caagcagctt tgggggatga agtccaagta ccaactgtcc atggagatgt gaaattgaag
    33361 atccctgctg gaacacagac aggtacgaac ttccgtttac gtggaaaagg cgctccgaag
    33421 ttacgtggtg gtgcaacagg cgaccaacat gtcaaagtaa aactgatcac accgaaaaat
    33481 ctaaatgaac aacaaagaga agcattacgt gcattcgctg aagctggcgg catcaaagtt
    33541 gaagaacagc aagaagacgg tttcttcgat aagtttaaag atgcttttgg cgggaaaaag
    33601 cggaagtagt ttttctagta aaaacgctaa gtcattaata attaaaaacc agacgaacaa
    33661 tcatatgttc gcctggtttt tttgtagttg tcatgtttgt accaacaaaa a
//
LOCUS       DACELF010000022        33155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.022, whole genome shotgun sequence.
ACCESSION   DACELF010000022 DACELF010000000
VERSION     DACELF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.022"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..511
                     /locus_tag="IW687_000997"
     CDS             <1..511
                     /locus_tag="IW687_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0950277.1"
                     /translation="SLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVFQG
                     ANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDAHY
                     KYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQCE
                     LVNVEKINK"
     gene            517..1683
                     /locus_tag="IW687_000998"
     CDS             517..1683
                     /locus_tag="IW687_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0950278.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            1765..2436
                     /locus_tag="IW687_000999"
     CDS             1765..2436
                     /locus_tag="IW687_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0950279.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            2489..4465
                     /locus_tag="IW687_001000"
     CDS             2489..4465
                     /locus_tag="IW687_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0950280.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            4478..5230
                     /locus_tag="IW687_001001"
     CDS             4478..5230
                     /locus_tag="IW687_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0950281.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5246..6007
                     /locus_tag="IW687_001002"
     CDS             5246..6007
                     /locus_tag="IW687_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950282.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6020..6280
                     /locus_tag="IW687_001003"
     CDS             6020..6280
                     /locus_tag="IW687_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950283.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6277..8367
                     /locus_tag="IW687_001004"
     CDS             6277..8367
                     /locus_tag="IW687_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0950284.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(8403..10460)
                     /locus_tag="IW687_001005"
     CDS             complement(8403..10460)
                     /locus_tag="IW687_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0950285.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(10605..10796)
                     /locus_tag="IW687_001006"
     CDS             complement(10605..10796)
                     /locus_tag="IW687_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950286.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            10984..11439
                     /locus_tag="IW687_001007"
     CDS             10984..11439
                     /locus_tag="IW687_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950287.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            11436..11678
                     /locus_tag="IW687_001008"
     CDS             11436..11678
                     /locus_tag="IW687_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950288.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            11696..11998
                     /locus_tag="IW687_001009"
     CDS             11696..11998
                     /locus_tag="IW687_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950289.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            12067..14121
                     /locus_tag="IW687_001010"
     CDS             12067..14121
                     /locus_tag="IW687_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950290.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            14121..16733
                     /locus_tag="IW687_001011"
     CDS             14121..16733
                     /locus_tag="IW687_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0950291.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            16730..19108
                     /locus_tag="IW687_001012"
     CDS             16730..19108
                     /locus_tag="IW687_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950292.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            19169..19711
                     /locus_tag="IW687_001013"
     CDS             19169..19711
                     /locus_tag="IW687_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950293.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            19789..20121
                     /locus_tag="IW687_001014"
     CDS             19789..20121
                     /locus_tag="IW687_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950294.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            20122..20715
                     /locus_tag="IW687_001015"
     CDS             20122..20715
                     /locus_tag="IW687_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950295.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            20737..22431
                     /locus_tag="IW687_001016"
     CDS             20737..22431
                     /locus_tag="IW687_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0950296.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            23119..24996
                     /gene="ltrA"
                     /locus_tag="IW687_001017"
     CDS             23119..24996
                     /gene="ltrA"
                     /locus_tag="IW687_001017"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0950297.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            25127..25504
                     /locus_tag="IW687_001018"
                     /pseudo
     CDS             25127..25504
                     /locus_tag="IW687_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            25504..26703
                     /locus_tag="IW687_001019"
     CDS             25504..26703
                     /locus_tag="IW687_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0950298.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            26719..27363
                     /locus_tag="IW687_001020"
     CDS             26719..27363
                     /locus_tag="IW687_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950299.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            27374..28180
                     /locus_tag="IW687_001021"
     CDS             27374..28180
                     /locus_tag="IW687_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950300.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            28203..28432
                     /locus_tag="IW687_001022"
                     /pseudo
     CDS             28203..28432
                     /locus_tag="IW687_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28591..29526
                     /locus_tag="IW687_001023"
     CDS             28591..29526
                     /locus_tag="IW687_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ0950301.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            29570..29839
                     /locus_tag="IW687_001024"
     CDS             29570..29839
                     /locus_tag="IW687_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950302.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            30115..30390
                     /locus_tag="IW687_001025"
     CDS             30115..30390
                     /locus_tag="IW687_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950303.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            30432..30854
                     /locus_tag="IW687_001026"
     CDS             30432..30854
                     /locus_tag="IW687_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950304.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            30874..32466
                     /locus_tag="IW687_001027"
     CDS             30874..32466
                     /locus_tag="IW687_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950305.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            32487..32822
                     /locus_tag="IW687_001028"
     CDS             32487..32822
                     /locus_tag="IW687_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950306.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            32968..>33155
                     /locus_tag="IW687_001029"
     CDS             32968..>33155
                     /locus_tag="IW687_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950307.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11949 a         5187 c         6801 g         9218 t
ORIGIN      
        1 cagtttaacg gatatttcta agagacaact gattactaat caaaaacagg cattagaaaa
       61 ggatctcaaa ggtgaaattg ctggaaaact tgttttttct tctacaggat ttattctccc
      121 tgtttttcaa ggagcgaata gcgacacgct tagtttagga gtagcttcta cttattatct
      181 agatactgaa atgggaaatg gaaattatat tcttgctggt tataacgtag atacaccaag
      241 cgttttatta tccgatatga ctgatttatc tgaaggagaa gaaattgatc taatcgatgc
      301 tcattacaag tatcagtatg aagtctccaa aagaggaaaa atttctgatt acagccaggt
      361 agttcaatct aaggaaaaca attccttttt atccttgcca aaagcaggta aaaaaccact
      421 actcacactg ttagcctttc aagcaaataa tatgaaaaag tgcgatgtga tccagtgtga
      481 attagtcaat gtagaaaaaa tcaataaata agggaagtga gtcacattca agaacttgta
      541 gaaaaattga tcaataaaat gagtgccatt tttctgttac tgttggtatc cattctatat
      601 ttaatatgga cggtggggtt aaatcctcct acgatattat ttatttatga taatgctgca
      661 cagttagtga cgtttttcgt aggctgtttg tttcttttga atctgtacaa ggtaaagaga
      721 aacgatctat tcttcattgc agcaggcatt cttttttacc ttttcttttc tttttttaga
      781 agctaccgag gagatacttt ctatggtgat ctgatcgttc cgacaattat tgctattatt
      841 ttgattgtaa aatggacgaa atttactgta attgatcgta gcttgtatct tgtagtattc
      901 tatgtctttt tactcgtaac ggtttatcga gtatttactg agataccagt accagaagga
      961 caaagtattt ggaatccatc gaacaaatta tcagatattt ggatcaatac aaatacgata
     1021 ggtagttctt taatgacatt agcccttctt atcacaggat ttgctagttc atttggaaaa
     1081 tggtacatgc gattgctatc attaccagcg ttgattcttg ctggggcaac gatttgggtt
     1141 tgccaatcga aagcagcatt atatgcgctt gttatcttca ttatcttaga aattttacct
     1201 aagaaggttt ttcgaaatac acacttgtcc ttttggatgt atgcagcagt ttcggttcta
     1261 gctttaccga tttcttattt tgctgcaatg tcagacaaag ttcacttatt tacaggacga
     1321 gaagaaattt ggcgcaaatt ctatgaaaca ttaggcgaaa aaacaggaca aatatttgta
     1381 gggatgaaac ctttcttttt ccatagagga aatcagattc taggtaatca taatacgtat
     1441 cacgcactcg tcaatcacta tggattgatt ggttttgttc tatcgattat tcttttgctt
     1501 agtttcttat ggttgctcgt aagaaaacgt gagctgacaa atggacaact aacattcttg
     1561 tgggcttttt tagcgatcat gtgtcaatcg ttcatggaag aaacattaac gacaatcagt
     1621 tgggtgccaa ttgtattctt aatgttagca atggctacac accaatataa aaccaccaga
     1681 taaatgaaat agaatagtaa taagagaaaa cactttttcg ttgtgaaaaa gtgttttttg
     1741 ttttgtctga aaggaggcag atggatgaaa aaatggttat ttggtttttt aggagtcgct
     1801 ttaatcgtcg tttgcagtgt gtttggctat gttagctacc aaaaacatga aggagaggtg
     1861 tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca cgcaaagagt
     1921 tataaggagg atgcaacgaa cgtcaataat gatatgtctt tggggcaaat gttgtccata
     1981 caaaaagaag caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat tcagattcct
     2041 gcacttggtt tagcattgcc aatttttaaa ggggcaaatc aatacacttt gagtcttgga
     2101 gcggctactt atttttatga agatgccgaa atgggtaaag gaaactatgt cctagcaggt
     2161 cataacatgg aaatgccagg tgttttattt tctgatattc aaaagttgtc attaggggag
     2221 gtgatggact tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg taaatttatc
     2281 gtacctgagt actttaaatt aatcgatggc gttcctgaag agaactcttt cttgtcttta
     2341 ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac gtcacaagga
     2401 aaagaaagat acgtggtaca gggagagcta caataacaag aatttaatca acgcgagtcg
     2461 ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg
     2521 aatgattgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc gagcttatgc
     2581 agaagatgtc acgcaaaaaa cgccaccaga aagggtcaat atcacggttc ataaattaat
     2641 gtacgatcaa agtacgcaat tgaacgttga tattgacgga atcaaaaatg atggttatac
     2701 gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg gtgatgttga
     2761 gtttacgtta ggaaatatta cggatcaagt attaccgact gaagattctg atctaaccaa
     2821 cgcgaaagtg gacgaaatcg ttaaagatgt agaagacaag ggatctaaca gtgagtacgt
     2881 taagaatgca accaataaaa ttacttcagc tgtagatgaa aatggtgaga tcacttttgc
     2941 tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg aaagtaaatc
     3001 cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc caatgacaga
     3061 caacacagga tcaggatttt taaaagatat tcatttgtat ccaaagaaca ttgtttctaa
     3121 gttgagtttt gaattaacga aatttggtga cgatggcaca gctcaaagca aacaaacacc
     3181 tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa caaaattagg
     3241 agagcttgta tcggatgatc aaggaaagct gactgcaact gatttgacgt taggaaaata
     3301 ctactttgtg gaagttcctt cagaagttgt agttggatca gatcaagaac caacagcaga
     3361 ccaatatttg ttaggcgcag acgctcgcaa cgatgcgcac aataagttaa cctttgaaat
     3421 tacgaatgac ggcgtgacaa gtgatttaaa agcttcctac gtgaactata aagcaccagt
     3481 gatcgataaa acagtgacaa atggaacagg agaagaacat tctttccaaa ttggtgacgc
     3541 agtgaattat caaggtacaa ttcatatccc tacagatatc gcaggtggaa cagatgggat
     3601 tacagtaaat ggtgtaaaaa gtgaaacttc tccttacagt gtatttaaat ggggcgacac
     3661 agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa caaataatga
     3721 tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg atagtggttt
     3781 tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga atttacatgg
     3841 tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa tcgcaaatcc
     3901 attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg aagaacacca
     3961 cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag tcgatagcgg
     4021 cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg cagctaagaa
     4081 tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg tcaaagaagc
     4141 agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag agggtcattt
     4201 tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag ctcctgaaaa
     4261 ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat acaaagaaga
     4321 aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg gtagcaacgg
     4381 tttccaatct tacgtattga tctcttgctt gttgcttggg gcaggcgcaa tttctgcagt
     4441 tgtttacttt aagaagaaag cctagaagga tcagctaatg attatcagac accctaagaa
     4501 aaagcgaatc atgggtaaat ggatcattgc tttctggtta ctttcagctg ttggtgtctt
     4561 acttttgatg cctgctgaag cgagcgtggc gaaataccaa caaaatcagc agattgccgc
     4621 aatcgatcga acaggaacgg cagcagaaac ggattcttct ttggatgttg ctaagattga
     4681 gttaggcgat cctgttggta ttcttacaat tccaagtatt tctttaaaac taccaattta
     4741 tgacggaact tccgacaaaa ttttagagaa tggtgtaggg attacggaag gaactggcga
     4801 tattacagga ggaaacggta aaaatccttt aatcgcaggg cacagtggac tttataaaga
     4861 taatcttttc gatgatttgc cttcagttaa aaagggagaa aagttttata tcaaagttga
     4921 tggtgagcag cacgcttatc aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca
     4981 aagaaacttt gtcacttact tagaaccaaa tcccaatgaa gatcgagtaa cgctaatgac
     5041 atgtacgcct aaggggatca acacacaccg tttcctagtg tatgggaagc gagtaacctt
     5101 tacaaaatca gaattaaaag atgaagaaaa taagaaacaa aaattatctt ggaaatggct
     5161 tttaggaagt actgtttttc tttcagtcat gatcattggt tcgctatttg tttataaaaa
     5221 gaaaaaataa aggagggtaa ccaacatgag aaatagaaaa tggctatttg tttgtgcagc
     5281 cgctcttttt tctttcactg cctacacaca gttgacgttt gcagacagac aagaaagcac
     5341 ggatagcaca gaacaaacaa tccaaacgtc agatagcagc acgattcaaa gcagcgatga
     5401 aaccgtcgat tcgtcaacag ttgattcttc agaagaacct aaaattgctg aagacgatca
     5461 aatagtcaaa cagaccattc acgttcaaaa aattatttct tccgaacaat tagacaacca
     5521 ggggcaaaag cttgaaaaac aagaaggagt aaatggcgcg aagttttctg tttatgatgt
     5581 atcggacatt ttacaaaaga tggatgttaa ggatctcact actgaccaaa ttgaatccca
     5641 attaaaagat cgagtgaaaa aactatccag tgatcaactg aagctaattt ctaatggaga
     5701 aacaaagaca attgatcaac aaacaggtgt ctttgaattc tcggtagaag tacaggcaaa
     5761 tcagaaacaa gcttattata ttgtcaatga atcaagtcct gaaaacattt ctaatagcga
     5821 ggatattttg ctattaacac ctgtttctga taaaaatggt gaattcttaa aggatgtgtg
     5881 gatttatcct aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa tcgtatctac
     5941 tggcgtaaag aaaaacttct ttgagaattg ttgggatttt gttactcact tgttttctag
     6001 agattaagga agaaataaaa tgaaactgat caaattatta ttattcaatt tttcttgttt
     6061 aaccgcagtc tttttgtact gcggtttatt attttctaaa ctatctattt tgccaagcta
     6121 ccaactatac ctaatgagga ttttaaacat accagctgct tccatgccgc tagatattat
     6181 ggaaaagctc attaggaatc cattatttac tacgatcaat ttgatcgtta ttgtcggaat
     6241 ttcactagtc atttataaaa aagaggggat gaagggatga attttaaaca ctcgtggaca
     6301 agacggttaa aatatgtttt tcccataatg atgatgttag gtacattgtt tgggttaaaa
     6361 acagccaatg tctttgctga aaaagttatt gtcgatccgt caaatccaat ccagaacgta
     6421 aagaataatt ttattattcc aaaagatgtt cctaatgcaa aaacaaatat tactgtaaac
     6481 ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca aaggtggttt tacagacgac
     6541 tacgtaggat tagtggaggg aaacaaaaat atacgctatc cttctttcca aaaagaatac
     6601 ggtgaaacaa accttgtttt agaaggcgta acaaccaaca ccaaagtcaa tattgactat
     6661 ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca agctagttct atctaatatt
     6721 cacttatact cggatacgtt gccgtggaat ctactagata acaactatac aaaaactcat
     6781 tttcgagacg atggctataa aaatacgaat ggtgcaatga gtaaatctaa aaaaagaaca
     6841 gtactttgga tctcagacaa tcttttctca ggaattgttt accattcaac acaaatgaat
     6901 gtgcagctag tagccacata tgaggacggt agccccgttc aattttcagg agatacattt
     6961 atcagtttta actctttgaa ccctgcagga ggaaaaagca cggatcttaa aggagaatat
     7021 gcccactatg acaaaatgaa tacgacagat tggtatgtca gaaaagatac ggttctttca
     7081 gaatttaaat ctttctacaa caatctgaac gtggtaggtg gccacccagg aggaagctct
     7141 aagctgacac aagcggataa tgatttcaat aatttgcatg ataaattagg agatccgaag
     7201 tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa atccgacgtt tgtgattggt
     7261 tcttctaatg ttcaaacctg gtttacttta tcttctgcga caatctttag tgttgttcct
     7321 gatcaacctg aaaaaacagg ggttgataaa aatggaaata acgtgaacga taaaatgttg
     7381 caagtaggag acacgattca atatcgaatc aaacagaagg tgaatcgatt aggagtggat
     7441 ttactagcgg tttacgatcg atttgagtta atcgataatt taccaaaaga agtaaattac
     7501 gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg atgtatctgg tgaagtgacc
     7561 tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag ctgatacatt aaaaaatcga
     7621 atgaagtata acggcgaaac ttacgaatta gtgatcaatg taaaagtcaa tgaattggcc
     7681 aatcaaaata gtgtagccaa aaatcaggga acgtcgatca ttaataaagt agaaaaggat
     7741 acaaatatca ttaccgttta tttccctaaa attccagtga aagaagtaca acaaaatgga
     7801 aaagatgtaa acggacgaaa tgacggtaag aagggtacgc cgacagctcc tttaaacgca
     7861 ggatctgaag tacaatatct agtcacacaa aaatggcaca caaaaggcgt agacgctgcg
     7921 tcagatcact acaaacaatt tagtattcaa gatccaattg aagctcgttt gacatataaa
     7981 gaaggttctg cgcaagtaat agataagagt aaaggaaaag atattacttc tgaaggcaca
     8041 ctaacctatg atagcaattc aagaacgctg aagtgggaag cttccgctga cttcttaaat
     8101 aacaatcttt tagatggacg agaaattcag ctgatattta cagctaaaac tccattacaa
     8161 tcagaaaaaa atattgataa ccaagccgta gtagcagtag ataatgtttc taataaaaca
     8221 aatgttgtga caattggggt tgatcctaac ttgccacaag ttattgttcc taaaacaggt
     8281 tctacacatt tagtaacgat tttagcggtc agcttactat tacttgtgtt agcaactttt
     8341 ggctacgcag ttctgaagtt caattaaaca aaaaaacaag gctgaaaatc agccttgttt
     8401 ttctatttga aaatgggatt ggcactaata aatacgttgt ctgaagaaag tgatccgtct
     8461 gcagaaactt cataagcatg ggataaacca acgatacttt gattgttgta aagcaagttt
     8521 gcgttatgag cagcgctatc tttccacata accatagcat ttactgctcc tgtatctgaa
     8581 aggatctcgt caacgtaaag gtagaaacta ttgatatttt ctcctttagc accgtagcag
     8641 cctaagcttt ctaagagatc ttggaaagta ccgccgccag gtcggtaatg actgaagctt
     8701 acttgtaatt cttttgcacg aatatctgca gcttgttgaa cgatatcatt gaattgcatt
     8761 ggggcaacgc cattttgttg acggtagcta tttaaccaag caagttcttt ttgacgcaca
     8821 tagttgttta aatcacttag tgagatattt ttgtatttga aagtgatcgt tcgattttgg
     8881 tcaacattat tgattacacg ttgtccattt ttatcaacgg taacagatga atcgtcattt
     8941 ctacacaaga agtagttttg atcggtcata tttttccaag aagtagagaa actatctccc
     9001 tcgtaaaccg tttgtgtttc agtagataaa acagcgtttg tgtcaacgtt tatatggttc
     9061 acagtgacgt tgtattgatg agtataggca aaattgattc cttgattact tgtaacgttg
     9121 tttaaagtca cagtgttgct agagctttct ctaggatcaa gaacaaaatt agtgaacata
     9181 ggtgcttcaa ttgtgatgct ttgtccataa acgacgggat aactcttact gtaattatag
     9241 agttcttgga tatttggatc agtatccgag actttatttg gacgagcttt cacttcaact
     9301 agaaagactt catcatagta aaaagtaaat gtggtatttg tgttaatcgt ttgtgtttgt
     9361 gtttcattcc catttacttg atactgtggt gcttcaaagt tagtcaagtc ggttctagct
     9421 tttgcagtgt aacttgtacc agcttttact gagacatgtt cttcttttag cagcgttcca
     9481 gtttgtcgat tcatatgacg gacagttacc gcatattctg aaggtttagg tttgtgttgt
     9541 aaccctttaa atgcagaatc aagactattt ttagcattat ttacttgggt ttgagaagct
     9601 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     9661 ccttctgtaa aatctccttt agcagttgat tttacttggt tgtacaaggc ttgtaattcc
     9721 gttttgttca cagtaggagc tggttttttc tttaatccct tgtaagcaga atctaatgtg
     9781 tttttggcac tatccacttg tttttgagtc gcttttgagt catttaacac ttttttagca
     9841 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttagtt
     9901 cccttaacgc tcgtataaag agcttctaaa gctgatttat tcactttggg tttagaggtg
     9961 tcagcaccat accaaccaat accttcgtat ttccagccat agtctttggt taaccattgt
    10021 acttctttcc aacttgcagt gtagtgatgc gaaccagagg taacacctgg aaggaaaagg
    10081 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccaa
    10141 ccgtaatttt tggtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
    10201 aggcctttat tgtataaacg atagacaagg tggccaccgc tagtcggtgc ttcccaagcg
    10261 gggccttcat aattacccca cttaattttc actaggtgat cacgttcttt tgtgctagag
    10321 gtgtaaaaat gttcatggtt atttggatta taaaggcgat acatgacgtt agttgaagca
    10381 gcgtcagcag atccagcaag agcaactgtg gcagcaaatg ttccaatagc tataacagtt
    10441 tttgggttta ataatttcat gttttcaact ccctattaaa tgattaaata ttttgtttac
    10501 attcataata taccacggta taccgaggta gtcaacggta taccgtggaa attttgaaat
    10561 aaaaaagccg ccctatgaag agtgtaagga tggctgttta tatgctatga agaaatggat
    10621 tcggcgtatt tggttagttt ggcagcatta tcaaatcgca ttttttcaat cgcacttttt
    10681 ttctttatta aatccgcaat tgttgattct gcgactgaag aatgtttgct aatttcatat
    10741 gctgtcttgc cagtttcaaa tagtaaccat tcgattttct ctttatcagc gtacattgca
    10801 atgctccctc ttgatttttt ctttattata gctctttttt gtaataaatc acaatatcaa
    10861 gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat ccgttccccg tgtatcaaaa
    10921 tgaggtttgt tgctactttc gttatttaat atactaaaat aaaacaacga aagaaggaat
    10981 agaatgaaaa agttaataat ggtatttact tcgttgattg ccatgattgt tattttaggt
    11041 ggatgtggta gtgaaaaaaa taagcaagat agtcaagata atggcgtgtt aaaaccagga
    11101 acagtgtgga aagaagaagt aggcggactt gttcataatt taaaaattat agatgataca
    11161 acatgggaat attccgaaag tgtttggcat cctgaacaag tacaaataac agttgagaag
    11221 ctaaaagact ataaaggatt agaaagatat aaggtaatcg attcaggtgg agtagattta
    11281 ttcataggta aaacagcctt aatagttccc tataacaaag acggtttaga aacgtttaaa
    11341 tttattccaa caaacgaaaa agaacatcga gataaaagtg aaatattaaa agaatacagt
    11401 actaaatctg gatacaagtt acaaaagact agcgagtgat tgctagtctt tttatttatc
    11461 ctgaaggagg aatcgcagtg gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa
    11521 tcttagaacg tcgcggtgta tctgaaacac agtggaaaca agaagtcctt gaaaaagcac
    11581 aatttgacct attacgggga aaagacaagg acttagaagg atatatctat caacgtgaac
    11641 gtgaaaagct tgttttcgat gaaattatta aacactaaaa ggagcgaaca attttatgtt
    11701 tgaatcaatt acaatgaatt ttttaacaca accgaaagta gtagctgcgg ttgatatttt
    11761 tagagcatta caaggtgcag caactagttt aaaaaacaat ctgatgattt ttgcggctgt
    11821 agctgccgtt gcctccattg ttcttggggg gattttcttc ttctttggcc gaagaggagc
    11881 tgaaaacggg aagatgcacg taggttatgt attgatgggg gtaggagtta tattagctgc
    11941 cgcctctatt gtggtttcgt tatttggaat gtttggacaa accccacagg gactataaaa
    12001 aataaataga aggagtaaaa agtacgtggc tactttttac tcctttttta tgaaaggaga
    12061 atactcatgt actttacgat ttcaggcaat accaaaaaag ataaagagtt aataaaaaaa
    12121 gcaacgaaga aagtgggaaa gcgtactgcc tttttaccga aaacaattaa atattctgtc
    12181 attgaaacgg aagaacagct acatgtgtta ggacaagtgt tagaacaaga gaatcaatct
    12241 tctgttaaat tgactctcac gttacatgat aaagaaggaa aaatagaaaa ggctggcaca
    12301 gaatacgttt ttggcccact ggattatcaa atttcagaag gtttttatcc tacattgatg
    12361 gatttgatcc gccttgatgt gtttaaggaa acggatgaaa ccatgagtgt ggaagagtct
    12421 tatcgaaacg aaaaactgct acaagtttta gaagaagagt tagagagcga ccaaagaaaa
    12481 gtgagtcaag tgagtgaagt cgaagtaaat gatgaagtac catgggaacg accaatcttt
    12541 ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg acgaacaagc tgatattccg
    12601 actcagaaat cgatatttga ggaagagaac gatgtaatgg gtcaattgga ggtagatccc
    12661 tttgaagaaa caatggagga tgaagagacg gccgaagtag aagttgatct agttgaagct
    12721 aatgaagagc caacatggac tgaaggggat tctcaggaat atattgaacc acttatcagt
    12781 gaagaagtag agaaaaacga aacagagata caaaaggcaa ctgtatttga ttatattttg
    12841 gatcaatatc cgccagagca tgaatggatg aaagaaaaaa ataaattatt tttaaatgag
    12901 ctttattcga aatatcaatt accccaaacc tatcaagcat ttttagagag taaagaagaa
    12961 ttaatctcac agggtaaagg cacactttta gaacagttag agtctgttca atcaacaaat
    13021 tggcatgagc ttgcacaaga agatttatta gccacttttg aagaacgtca acaagaaaca
    13081 gatcaagaaa taaatgagta tgcgacggaa cagtcatcta attgggagag aacaaaagag
    13141 gaactaaata aagaagaaca acgagtgatt gaagaagaag tacaaaaaat taaagagcga
    13201 tacgaaaaga aacgcgacaa tgcgtattcc ttgtgtatag agaaaattaa taactttact
    13261 aaaactaatc aagaagcttt atcgaaagaa aaagaaaact tgttaaaaga acgtataaaa
    13321 gaacgaaaag aagagttcta ccgaggttta cgtacagata agctaaagat tagcaaagaa
    13381 ttaaatgaac aactgaataa actttatcaa acaacgactg aagttcttat ggaaagacac
    13441 gggcagatcc agcaagaatt acaagcacaa attccaaaat ggaaaaaaga gtatgaaatt
    13501 caacaacaag aattgaaaga acaagaagaa aaacaacgac aagaagaaaa agagcgcgaa
    13561 caattagaac taaaaaaacg tgagcagcat cttgcagaac aaaaagtggc gatagaaaaa
    13621 caacggttag agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa
    13681 atggatacat tgcgacaagc acagttgaat tatctagcac aacctcaacc agtagctgct
    13741 gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt caccacagcg ttcctttaac
    13801 ggatggatgg tgggatgtat tgcaagttta agcttgcttc taggtggagg aacagtattt
    13861 gcttttaatc atatcaatca atcttcacag gcagattctg tagccacaac ggcgtcaatc
    13921 aaggaagaag cccaggaaca agcaagaaaa gagtatgaac aacaattgga agaattattg
    13981 aatcgtacca atcagtcaac agcagagtca agtatggaaa atagcaaaga gagtgatcct
    14041 agtaaggcta aagaaggatc aagcgattca actgtacact caaacacttc cgaaagttca
    14101 gaatcaaatg aggtgaaata atgcgtatta gagaaacaac gagacgaaaa gacaaacgat
    14161 tcaatttaga aaagaatagg tatctttctc atttctatcg ctatactcct tcaggagaag
    14221 agctaccaga aaaacatgac aaacgtattt ctcaaaagat tttttatagt ggcttagctt
    14281 atcttccttt aaactatgtt gttacaagaa taccagaagc atttcatcga tttgagatta
    14341 ttccaggaag tttacaagga gtgaatattt ttcagttaca agaaatgccg cagttattcc
    14401 attttgattc aactggtttt tggaatcctt tgaccccaac aacaagttta ggcacacgtt
    14461 tagttgtttc tacgatttgt gttgttccag ctgtacttat gggattgaaa atggacatgt
    14521 ggtttcgtcc gctagcagat gggcaaaaag gagataaccg attaaaaacg ttaaaggaaa
    14581 tccaacaaat ttatccgcaa ataccagaat caaagggaag ttttcctggt tatggcggaa
    14641 taccaattac tcattataaa aaatactggt atattcagac ggatacagta aatacttgta
    14701 ttgttggtac ttcccgctca gggaaaggcc agatagaagt tttagcaacc attgacaatt
    14761 tgtcgcgtgc agaaagacaa agttcaatgg ttgttaatga tccaaaaaca gaattatatg
    14821 ttgctagtaa agatgaacta gaagctagag ggtatgatgt gtatgccttt aatatcaccg
    14881 atccactgca atcaatgagt gataatccat tggcactcat tgtgaaatac tggaagcgag
    14941 gagacataga caccgcaaca cagttaacca atacttttac gaatacgatt tattatgatg
    15001 caaatgcttc agacaataaa tactttaacg aaaatgcgca gaaagctgtc aatgcgctta
    15061 tttttgcgtt attggaacaa gcggatcaaa caggggatta ctccaaatta acaccaaata
    15121 atctcgttga actactttca gaaattggcg gttttaatta ttatccagat cctgagaatg
    15181 aattcaagca gctcaatgcc ttagatgagt tcatgaatca gttaccccct ggaaatatag
    15241 caaaaaaaca atatggggca acaaaaattg gatctgataa agcgaaaggg aatatcttgt
    15301 ctacggcaat tacaggattg aatccattta ccctaacgaa aattgcgaag atgacttctc
    15361 aatcgaccat tgattacaag tccattggtt ttccgaagta tttggatctc aaattacatg
    15421 aaagcctatt aaatcaacgc attgtcgtgg aattttatca taagggaaag aaatttcacg
    15481 aggaacgagt caaagttggc tatcgtggtt tttgtgaaat caactttgat tgtcatttag
    15541 agcagggaga tcatgtttgc ttaaaataca atcatcaagg aaaacaatat gttgcttggt
    15601 atgagttttc tcaaaaagta ttgaaagacg aagagggaaa aatcgtttat cgaaaaaaac
    15661 gaggagaaac tcatttgcca gaactagaaa aagaagccgt tctaacatta gatgagaaga
    15721 aaagtaacct attagtagag tctattcgga tgcactatac cgataaacca atggctattt
    15781 ttatgttaac gcctgattat gatccatcta accatatcat tgttagcatc tttcttagtc
    15841 agttgtataa agaattatct acgcaatgtg ttaagactaa aggagataaa tgtcaccgta
    15901 ggattcattt tattttagat gaatttggaa atatgccacc gatggacaac atggaaggaa
    15961 tcatgacggt tacggcaggt cgtaatatgt tatgggatct gtttattcaa tcttatcagc
    16021 aactttatgc gaagtatgga gaacaaaacg cagcgattat caaagaaaat tgtcagacac
    16081 atatttatat tatgtcgaca gatgataata ccatcgaaga attttctaaa aaagtgggga
    16141 ataagacggt tgaacaagag aacagtacca tcaattcatt aggtatgaat actcatatca
    16201 atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag aatttctact tttttagaag
    16261 gtgaaacgat tgtactcgct cctttaaaac gacaagattt gaaaggtcat aaaatacggc
    16321 cgtatcctat ttttaatcat ggggaaacga atcttcctta tgcttatcaa tatattgaag
    16381 atttcaatcc atcgtgggat atcaatgata tagacataca ttgtacacat gcggacttgg
    16441 atcttaaggc tttacaaatt gattatgatt atttcctacc agatgaatat gctagaaatc
    16501 tctatcatgg actgcatgat caattagaag aaaaaaacga agaaaatatg ttttataaaa
    16561 tcgttcaatc ttttgagaat gagacggcta aaaaattgtt aattcaattt tacgaagaac
    16621 aagcaacgga tcgtcttcaa gaatatgttg aaaagttgat caaaaatgaa gaaatttctg
    16681 aaatgattag gaaacggctg ttattggaag ctgaaaaggc ggtgaaagaa tgatcattgg
    16741 aggaattttc tctggcatag ccgactggtg gtcaggttta ttcggagaag gggatgcaga
    16801 tcccaaaacc gtaacgaaag tcttacaaga ttatggtaat tatctgcagt atcaagatgt
    16861 attcagttgg atttgtaata cttttttatg gggattgatc aaagggttat ataatctcaa
    16921 tactttatta gaaaaaacaa tttatcaaag ctttgattta aaagacttat tgaacgccgc
    16981 tggtgttaat gaactatttc aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt
    17041 cacattaatt tatttaggct tgaaattttt tatgagtaaa cacccaccga aagtcaaaaa
    17101 tatcttggtt aatttattta tggccatgat cttgattttt ggtggtagtt ctttaatcga
    17161 tgaagggtta aatatcagta aaaactttta tggagacgtc actgcagcaa ataaagcaga
    17221 taaaacgggt tcacctgctt ttcaaattat tcaaaataat gtgattgatg tcagtacatt
    17281 actagcgact gacccgaata aagtagaatc cttaccaaca gataaaagaa atgctttaac
    17341 agcgaaaaat ttcaaaacag cgaatatcaa tgggattatt acacctgatc aagctaagaa
    17401 aatgtctgaa tcaagtgata ttagtacagt acaaaaagat cggttaaagg cattacagta
    17461 tcgactagag ttagacgata atgggaaaga aatccctgtt caaattagtg atgaaggttt
    17521 agcaaaatat gtgtatacgt caggctatcg tcgatacatt agtagtccag ggattatttt
    17581 ggctggggaa tcttctttgt cggtagccta tttatttatt ttgtttacaa tcgttacttg
    17641 cattattgag ctagtattta aaaaattcta tttagtaatt gccgcgtcaa cggactttga
    17701 gacaggacaa cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc
    17761 gtttacgatt ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca
    17821 tgcggtagga aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc
    17881 gctattcaaa ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa
    17941 cggcagcaat tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag
    18001 cggagctaaa aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg
    18061 taagaatgga tttggccatc gaaaagatat tacccatgat aacggggaag aacaatcaga
    18121 cgataaagct acaactgtac ctaatagtaa gaagacagtt tccgaaatgt tcgggaaagc
    18181 gaaaaatggt gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa
    18241 aggaacagtt gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg
    18301 cacgagagct aaagaatttg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca
    18361 aaaagaaaaa gcttcagcag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt
    18421 caatgcagca gtgaaggcaa atacaacaag tgaaaaagaa aatcaagaag gtgcagcgat
    18481 tccttcagcc aaagatcacc aagatgagtt acattctaac gaaaattcga tcggagaaaa
    18541 agattcagaa gaaggacaac atggtcagtt accaaaaacc gatccagaac aatatcgtta
    18601 taaatctaac attaattctg aaaacgaaaa acgcatttca gatttaccaa aacgaaatca
    18661 agaagtacaa acggatgagg attccatcga tctgccaaat gataatggca tcgaagttgg
    18721 aactgaaaaa tcaacgaaaa ttggtttgcc taagacacaa aatttaccaa tgaaagatag
    18781 tcgtttgaat caattcaaag agaatgatca aagctttgtt catcaatcac ctgcaggtac
    18841 tgttcaaatg aaatcaagtc gtttgcctga aaatggaaat ctgacaactg tttcttacaa
    18901 tttagctgaa agtggtgaaa cagagaatgc gtccgttcaa aatattgtga caaaagaagc
    18961 tgcacctaag ccaatcaata aagttcataa tggatacaga ccagcaaacg tagcatttac
    19021 tgcaccgaca aaaataaatg tgacaagaga aactaaaaac aggaacaatg aattgaaaga
    19081 acaactattg aaggatatca aaccataaaa ggcctattta agggcctttt tctttataaa
    19141 acaagtttaa aatggaaagg agaaaattgt ggaggatatt ttaatagcta cgtttataac
    19201 tgcactggga gcatttttaa cgatatttgt ggtgactatt aaaggattta aagatacgga
    19261 tagtttacaa aaagatcatc agtctatttt agatggtcaa aaaacacata ccgaaagcat
    19321 tattgtacac ctttcagaag gattcactta tttgaaagat gctatgccca cattcacagt
    19381 aaaattaacg gaaatatttc attctgttga gcaaatatcg tcagaattga aagagcaaag
    19441 acaacaaaaa gatcgtcaac ttggctcact taattttagg caagaaacga tacaagaatc
    19501 attggagaat gtaaatcgat taaacgatga aatgcaacga gttcatttgg aaaatgaaga
    19561 attacgcgca caaaattatg atttaattca aaagaaaaga aatctggaac aaaaaattgt
    19621 tgaattaaaa gaagaaaatc gtcaattgaa ttatcaatta cgacgaggaa aaaatcgttc
    19681 tcgtgattta ggaaatgaat tagagttgta atatttagaa aaacgctgtg agaaatcaca
    19741 gtgtttttta tttctaaaaa tacaaaatat aaggggtatg gttatattat ggataaatac
    19801 attcatatga aaaatcctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt
    19861 ttagatctat taacgcttgg tggctgcttt ttttgggcga aaatagtcaa taatcttgtc
    19921 cacttaccac tcttttttgc actcttgaat tatggattat ggcttgcgat agcgttagtg
    19981 attgtttggc gaccctataa taaccctggt caacgccaaa tttttgcgat tatcgaatct
    20041 attttcttta cggataagaa ccactatcat gcgttagatc ctaaccgaaa ttatcatggt
    20101 acgtataagg aggatgaata aatgcgagtc aaaggacaaa attcagttga taagaaatcc
    20161 attttgcagt ttggaaaacc agaagagcgc tctatcttgg acttatttcc atataaatcc
    20221 tgtacagaag agggagtctt aattactaga gaagatcatt ttcagcgtta ttatcgtgtc
    20281 gtttccactg acgtggaggg attaaatgaa caagagaaac tagaacgaat gaatcagttg
    20341 accaccgtca tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg
    20401 gatctaacgg aacaaatcgt gcaaaagcgg cagttattag ataaaaatcg tatggaacag
    20461 tcactaggaa gaaatctaca gcgattaaga aaatatgaaa agaaattagt agaggagatc
    20521 caggaattaa aacgggcaga acaagaacgt cctgacttaa gcttcttttt cgttattgaa
    20581 ggaaagacaa tcaaggattt acaaactaga aatcgtcagt taatgcgatc tagtggcatt
    20641 ttagggttaa aaccactcta caaaaaagaa ttgattaaga ttctttatcg aatgaacaac
    20701 atgaatgacg aataaaaaag atggaggaaa tgattcatgc tagatgtgat aaaaacagct
    20761 ttttcaacgc aaagtaatca aaaggtgatt gatccattaa aacaacttgg taatcgtgaa
    20821 atagagattt taaaatcaaa aggttatgat ttagatttct taaaacaagt gatgccacaa
    20881 ggtgggttga attttgatga agaatatgtg acctatggaa atggttattc acgatgtgtg
    20941 acagtttatc attacacaaa aaatcctact cttttttggt tggccaatct catgaacaat
    21001 cctcatacag tggccacact agatgttagc acagatgaaa agcaaaaagt gattgatcaa
    21061 atcaaccgcg cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac
    21121 cgaaataaaa gtgcggaaga atacctggaa ctagcggaat atgcccgaaa attgacttct
    21181 aatggggaaa tttctaaaca agtcaaagtg agagtacatg tttacggaaa tacgatccaa
    21241 gaagtggatg aacgggcgca ggaactgatc gaaaatctaa aagggatgga ttatcgtgcg
    21301 gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa
    21361 caagctgaat ggtttggaaa tatcaaaggg caatcacttc catcgttaaa cattggcgga
    21421 ggattcccgt tccaccacca aacgctaaaa gatccaggag gcacacaata tggggaaacc
    21481 atgacaggcg gcccatttgt ctggaatgcc acgtataaaa atagtgtacg tttatctacc
    21541 aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg
    21601 gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt
    21661 cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga cgggatgatc
    21721 aatcctttag agatctttgc cacacggacg tatgatgaag catccagttt atatgacgat
    21781 ggttcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacgca cttagacaaa
    21841 gtagtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattc
    21901 aagacctact tgaatcgttt ttatgaagag tatggaacag taccacgcgg ttttacttcc
    21961 tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga agcttatccg
    22021 acgttcaaag aatttttagc ctttttagga caactggaac taccaggggc ttcccaagag
    22081 aaaaagaatc ggaaagaaga aatggagtct atcgtggaat ccttatgtga gacttatggc
    22141 atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt
    22201 gacattgact ctattacagg cctagatcga ggggtacaac aatgtcagat gtacatggcc
    22261 atgacgttga tttggaatca ggcattgatt caaggacgaa gacaacgacg cttaattaaa
    22321 gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga tgagtgccat
    22381 aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta aaaactattg
    22441 taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg cttccactga
    22501 tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct cggtgaagtc
    22561 ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc tttagaggta
    22621 aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt atgtacaaac
    22681 gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa tgggagagac
    22741 agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg ttgttatgaa
    22801 agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc atctaagtta
    22861 aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag tgcatactcg
    22921 atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga tggtgaaagt
    22981 agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg attcgtctgt
    23041 aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg tcacgagcca
    23101 attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa gttgcggcat
    23161 gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag tttgaaggga
    23221 gataatttca agaatctaat gcagattatc atttctgata acaacattgt cctagcatat
    23281 cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt aacgattaaa
    23341 gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg attcgcacat
    23401 tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa attaagaccg
    23461 ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca agtattagaa
    23521 ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa tagatcagca
    23581 gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga atatgtggta
    23641 gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat gagacaaatc
    23701 tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat gttaagagca
    23761 cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc acaaggcgga
    23821 atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg gatagcaaat
    23881 cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa tggagcttgg
    23941 gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga aatataccta
    24001 gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc gaaaaaagtt
    24061 ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc tgaagaaaag
    24121 tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac gatgaaatta
    24181 gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc acaaagcatg
    24241 attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa tagcaaagat
    24301 acctttatct atattggagt ctataacagt atggttattg ggattcataa ttacttcaaa
    24361 attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag accaagtttt
    24421 gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa gtacgaagga
    24481 tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc aatattacca
    24541 gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa taaatacacc
    24601 gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg gaagatatcg
    24661 tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc ggacaatcga
    24721 atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga gcttatctta
    24781 tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga tagctatgaa
    24841 aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca aagtgaaacg
    24901 attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa actcaatgag
    24961 ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg acgggaaaac
    25021 agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg tacggtgtgg
    25081 actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga ttaatccaga
    25141 tttcattgaa acggtaaatt atgtcacaaa cttccaacga gaaatgcgta aagtagaagc
    25201 aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata tgtcttctga
    25261 taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct ttatgcgtat
    25321 ggatgaatct attttacgtc atatagaagg attagttggt aaatcaatga cgaactcaga
    25381 attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg gctcaaaaga
    25441 aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg atggaggaaa
    25501 ataatggaaa accaaaatga aagtttaatc aaacagtatg ttaagcgtcg tgcaaagcgg
    25561 cgcttatttt tgtgggtgtt tggtacttct gcaggtttga ttactatatt gatcactgtg
    25621 tttgtgaccc tttttctaat acttgcggca ggttctattg ataattctga ttcaagtagt
    25681 ggtggcgaag cttttacagg agaatattcg gaaggcttac ccatttataa agaaatcaaa
    25741 ggaagaggcc ctttttctga tgagattgcg caatatgctg taggtgcagc agtgaagtat
    25801 aaattattac caagtgtgat cctttcgcaa tacggttatg aatcagcgtt tggtacgtcg
    25861 gcgtccgcaa gaaatgatct caattatttt gggattacgt ggtttgatgg gtgtctgttt
    25921 cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg gctggtacat gaagtttccc
    25981 aactcaaaag ctgccttctc ctattacggc tttatggtag ccacacaatc aaattttaat
    26041 gcttgtgtcg gaaataaaag ccctggcgca agcttattga tcttaggacg aggagggtat
    26101 gccgccgcag gaattactga agacagtgcc tattataaaa attgtatgtc tattattaat
    26161 acaaataaat tgatggagta cgatgagttt gcgattaaac actggagaga aggcggcgga
    26221 actggtggta cgattacagg aagctggggc aatccattcc caggaagtag cttagataaa
    26281 aattcgttca gtggaggaca actttttggg aaaaatccag gaggagaatt ccgacctaac
    26341 gggttccatg atggactaga ctttggttca gtcgatcatc caggaagtga gattcatgcg
    26401 gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt gggtgcgtgt
    26461 gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc aaatagtaca
    26521 ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat tgggatacga
    26581 gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc acaaggacat
    26641 gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag cagcaaaaaa
    26701 tagatcagga gaaaaaatat ggaaaataaa accttaaaaa tcgtcttaac agctgcaatt
    26761 agcgttagtt taatcggcaa tattgcgttg ttttggcagc ttaaagaaga acaagagaca
    26821 aatacctata aaattgaaga agtggctaaa aacgcgtctg ataagctcaa aaaaaattct
    26881 tctgaagcac aggcgaagat tgacaagtta aatcaaacca tcaatgagaa aaaccaagaa
    26941 atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac tgaagcacaa
    27001 agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag cagaaatgaa
    27061 ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt aagtcctgta
    27121 gacgatgatc accaacatga tgattacgga agttactctt ttacattagg tgacgtgcaa
    27181 acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt tgttgattat
    27241 acaatcggca atcctcaatt caaagatgtc aaaccacaaa aaataaaagg tggattaact
    27301 gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag actttagtta tttcactcgt
    27361 taggaggaag aaaatgagta agaaaaaagg agcaagacaa cgttttaaaa aacgaaaaag
    27421 ttatcttaga tggctgggga tctttatgat tctcggcttt ttgattcttg ctagtccgat
    27481 ttacacacat aaaaaggtag acataccaga tgaagtaagc acgaacgcat ttttaaacac
    27541 gaaggaactg acgatggttt caaaagaaaa aggagcgaac cagcaattag tttttgaatt
    27601 tgcgatcgac gatgaagatc agagcttatt aaaagaacta gctaatctta attatcgtgt
    27661 agaagtaaag acagctaaag gggattatca agcaataaaa gccaaagtga tcaaggtgtc
    27721 tgatgattat tttgtagtga agttagcaca tgtaccagaa gaatatattg ctatgcgatt
    27781 aactattatt cctgaaaaaa ttgatccaaa ggtggatatg caagaaccgc aagatctgat
    27841 tttttatatt cacgaagata aggtcaaaga tcacgtgaaa gacgaagatt atgaacaaca
    27901 tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagcac aaaaacaaat
    27961 tgaatccttc caagcaacca ttgatttgaa tgaagaatta gtgaagaaac taaaagatca
    28021 attgccttat caagtcgcag aagatcagga aaatactgaa aataagatta atagttacca
    28081 gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa gaagagagca ttcaaaaact
    28141 tcaagaaaaa attgatcgta tgacaaaaga aaatatttag aagaataggt ggagtaaaag
    28201 aaatgaagcg agcgaacttg gtcggcaaag atactttcgc tgagcagaaa gtattgatta
    28261 aagcctatct caaagttgta aaagctaaag aaatatgtca agaagtgaat tatctaacac
    28321 aacaatatct gagttgaaga atggtagaaa gagtttagat accttaaaat tcagatcatt
    28381 tgaagctcta tatatagctg ctaaaaaata ttttacagct gataataaat aaaaaattaa
    28441 ggtgggttct atgtgaaaaa aacgtatcaa aagtttttat ggattagcta tatcgtccta
    28501 tttatcgttg ggtttctcgt cagtgtgtcc gtgatcgtcg gaacgctttt tcttttgttc
    28561 tctgacgaag atctggtact taaggggtta atgagtacgt tatcgtgtgt catgttactt
    28621 tatttatgta tacagatcaa cgaaattatg ccccctcatt ggcaagaaaa gaagaaagaa
    28681 tttaaggaag aaatcgaaat aaaatccaat gactggattg aatacacatg gcttcctctg
    28741 aaaagaaata gcaaacgaaa ggaattttgg gcattgcaat tatggccaat aggaaaactt
    28801 tttatttata tggcttcact tatggttact ttaatcggtt atttggtggt ggtctatggg
    28861 gtggcctatt acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat tttcacggca
    28921 gtaatgacat taatgtgtgt catgtgcttt tctattttta gttacttact cgtccaattt
    28981 atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt tattggactt acttatctta
    29041 ggcagtatgt taacgactct aacagatcgg ttcgaacaat ctgaaaaaac ggtagagggg
    29101 ataaagaact tcttaacgac gattgatcaa tttataaatg gatatatgta tttgtttgaa
    29161 gtaggcttta ttctcacttt gtttggttgt gtatttataa aactcattaa aagatcaatc
    29221 gaaaaagaaa tggttattga tctaattccg acgctgagcg gctatgagta ttacgttgat
    29281 tttggaaagt atgaggagtt gatccatacg gatatcgttc acacgagtat ccatgaagta
    29341 accgttaagg gcatcagatc tactttaaaa gatggacagg agacgaaaac aatcgaccga
    29401 agaaaagagg tctacaattt ttataaagtt gaagcaagtc tacgatatgg cattgggaaa
    29461 ttgaggttag atgggagtaa tattcaacaa aacaaaccat atgttttagg aaaagtggtg
    29521 atttagaact tcctatgaaa gatgaataaa taaggagaat cttttgaaaa tgattcttct
    29581 ttacagaatt gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg agcagttatt
    29641 caaagtgaaa gaacatcata atcagttact atgtattaaa acgggtacga tgaacattca
    29701 ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgtcga ttcgagagca
    29761 acataagcaa gaaaaattaa tggctactta tagccaattg attgatgagt gggaacaatt
    29821 caaggaatct aaaacataaa attccattta aacaccttat tttttaaaga gcataaggaa
    29881 acgtgtaccc actttcaaaa taaagtgggt agataggctg tgcctatcac gtttctgaaa
    29941 aaaataaatg accccaaaat tttcaattta tgtgtaggtt tatgggtagc aaaaatatga
    30001 atgtgggcag caaaaagttc atttgtgggt agtaaaattc aaatgtgtgt agcaatgtgt
    30061 gtagctacac acattgctac acacgctata acatatagtg aaaaggagat atttatggaa
    30121 gtaaaaataa gagatattga tgaaaaaacg attcaaagaa ttgaccgaag atgtattgaa
    30181 ttaagtgaac gcaccgggac taaatggagc cgaaatcgat acctaaaagt tttgattgaa
    30241 aatgattttg accatgaact tatggatcat agaaaagatc aatttgatcg gttgttagaa
    30301 aaatttgtgg atattcaaac ttataatacg aaaattttag aagaatatat tgcaacaaat
    30361 aatcaattaa tcgccttgtt aattgaataa taaaaatagt attgtgaatt tataaaagga
    30421 ggaaatcaat tttggcaaaa gcaacaagaa aacatgtata tctttacgat atgcatatcg
    30481 aagtacttga tgaaataatt tctactcatg aagatatatc taattatagt gaagcagtca
    30541 gacatttgtg tatgatgtat gatccaaaaa tcgaagaaaa aataaatcca attcaaatta
    30601 aattgaatgc aatgggaaaa gaaatttcta tattaaccga aatggtttca gagtttgcgg
    30661 atcttcattt gaaagataca ggaatactaa ttggtacaaa ttcaactgtc tatcaagaag
    30721 caaaagagcg tgtggaaggg aaaataaaag cgaatcaaac gaaaaagtat tctcctaaga
    30781 tcaagaaacc aataaatgaa tctgttcatg ccagagtaga cccgtttgat ccaacaaagt
    30841 ttttaaaaag ataaaaagta gaaggggaac atgatgaata aatcgcctgc agtcattctc
    30901 acttgtcaat ttaccttgcc caataaaaaa tctttttcaa catacattga ttatatgaca
    30961 agagaaaaag cgctggaaga aaaggaacga cgcacgccgg acgaagaaca ggaactgcaa
    31021 cgaatcaaga atgctttaga ggatttttat attcctgaag gagaaacttt ttcggagagt
    31081 aagacagata aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga
    31141 atcaattttg acaagttaga ggaacaagat tttaccaaat atctttctta tatgacccgt
    31201 cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg aacaaaaaga ataccaacgg
    31261 ttgaaccaag caatgaaaaa atatcaaaca gagccacata aaaaagcttc aggaaatctt
    31321 cctggagttt tttctatggc ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaaa
    31381 caaatcttcc aaagagggca acaaaaaggc tcaatcattt atcaagatgt tgtctctcac
    31441 gacaattcgt atcttgaaaa aatgggcttg tacgatccca aaacagatac cttagatgaa
    31501 gaaggattaa aagcagctgg aagaaaaatg atggagacat tattcgagaa agaacaattg
    31561 aatgaaacag gttactggat ggcaacgatt caccgtaaca ctaagcatat ccatattcat
    31621 tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg
    31681 agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat
    31741 tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa
    31801 cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag
    31861 ccagcgcgtt acgatcgaca cgcaatgaac ttattaaatg aagtatataa aaccctacct
    31921 gacaaacgtt cggaatggca ttatggggat gaaaaacgaa ccaaactttc accatacaca
    31981 agagagaaac tcgatgagtt aaccaaatat gtgttaacaa aagaacctga atatcaagaa
    32041 tatatacaga tttcaaaatc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag
    32101 agagaatcaa aggatgcaga aaaaaatgct ctctttgatt tgaagaagcg cacaggaaat
    32161 gccattctaa cagaactgaa gaaacaggat agtcaaataa aacctttaat agaaagtatg
    32221 gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca gttaccaacg
    32281 aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga
    32341 gttgtcacgc aacggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa
    32401 taccacgcaa gtaaagccta cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga
    32461 cagtaaagac agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag
    32521 aaggatcatt aagagaagaa cgaatcgaac aacttcccga ttgtttagaa aaagagcatt
    32581 tgtttcagat aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg
    32641 ttgagtactc agctactggt gtgtactgtt gtaatcatgt gaagggtgaa aaatatttta
    32701 tcattcaaga atatgagggc aagttagcgc cttattatac tacttgggaa atgaatgaag
    32761 aggggatcaa taacttcccg tgtaaaagca ttgaagagtc tatttcactc acagaatgtt
    32821 agttgttaag tgaataaaag aagtaaaaga gtcctgaacg taattgtcca ggactctttt
    32881 tttcaatgaa ttgttttccg ccaattcaat gagtggtgcg atttgcgact acattatatc
    32941 aaaagaaaga gaagggagga agaagaaatg gcttattcaa cgattggagt tgttgttgaa
    33001 aaaagcagag acaatctcgt ttttgtgaca gaaattcaaa cgggcagagc ttttgtcgtc
    33061 acagacaaag cggcaaaagc gtatcaaagt ggcgatattt taacgttaaa tctgactacg
    33121 aagacgttcg tcgatgcggc agaagattat ccatt
//
LOCUS       DACELF010000023        32108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.023, whole genome shotgun sequence.
ACCESSION   DACELF010000023 DACELF010000000
VERSION     DACELF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.023"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..722)
                     /locus_tag="IW687_001030"
     CDS             complement(24..722)
                     /locus_tag="IW687_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0950308.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IW687_001031"
     CDS             complement(715..2067)
                     /locus_tag="IW687_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0950309.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IW687_001032"
     CDS             complement(2290..3690)
                     /locus_tag="IW687_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0950310.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IW687_001033"
     CDS             3851..4588
                     /locus_tag="IW687_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0950311.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IW687_001034"
     CDS             4713..5192
                     /locus_tag="IW687_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0950312.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IW687_001035"
     CDS             5189..5956
                     /locus_tag="IW687_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950313.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IW687_001036"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IW687_001036"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0950314.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IW687_001037"
     CDS             complement(6859..7461)
                     /locus_tag="IW687_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0950315.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IW687_001038"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IW687_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IW687_001039"
     CDS             8142..8726
                     /locus_tag="IW687_001039"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0950316.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IW687_001040"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IW687_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0950317.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IW687_001041"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IW687_001041"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0950318.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IW687_001042"
     CDS             10627..10806
                     /locus_tag="IW687_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950319.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IW687_001043"
     CDS             10828..11853
                     /locus_tag="IW687_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0950320.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IW687_001044"
     CDS             12028..13272
                     /locus_tag="IW687_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0950321.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IW687_001045"
     CDS             13425..14372
                     /locus_tag="IW687_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0950322.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IW687_001046"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IW687_001046"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0950323.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IW687_001047"
     CDS             complement(15274..15516)
                     /locus_tag="IW687_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950324.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IW687_001048"
     tRNA            15637..15709
                     /locus_tag="IW687_001048"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IW687_001049"
     CDS             complement(15828..16466)
                     /locus_tag="IW687_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0950325.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IW687_001050"
     CDS             complement(16589..17221)
                     /locus_tag="IW687_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0950326.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IW687_001051"
     CDS             17572..17871
                     /locus_tag="IW687_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0950327.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20531
                     /gene="acm"
                     /locus_tag="IW687_001052"
     CDS             18366..20531
                     /gene="acm"
                     /locus_tag="IW687_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0950328.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20803..21288)
                     /locus_tag="IW687_001053"
     CDS             complement(20803..21288)
                     /locus_tag="IW687_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0950329.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21768..22409
                     /locus_tag="IW687_001054"
     CDS             21768..22409
                     /locus_tag="IW687_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0950330.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22428..23063
                     /locus_tag="IW687_001055"
     CDS             22428..23063
                     /locus_tag="IW687_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950331.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23081..23896
                     /locus_tag="IW687_001056"
     CDS             23081..23896
                     /locus_tag="IW687_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0950332.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24215..25651
                     /locus_tag="IW687_001057"
     CDS             24215..25651
                     /locus_tag="IW687_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0950333.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25840..28287
                     /locus_tag="IW687_001058"
     CDS             25840..28287
                     /locus_tag="IW687_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0950334.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28517..29584
                     /gene="rlmN"
                     /locus_tag="IW687_001059"
     CDS             28517..29584
                     /gene="rlmN"
                     /locus_tag="IW687_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0950335.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29777..30880)
                     /locus_tag="IW687_001060"
     CDS             complement(29777..30880)
                     /locus_tag="IW687_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0950336.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31433..31981
                     /gene="aac(6')"
                     /locus_tag="IW687_001061"
     CDS             31433..31981
                     /gene="aac(6')"
                     /locus_tag="IW687_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0950337.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        11023 a         5470 c         6523 g         9092 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
    18601 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
    18661 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
    18721 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
    18781 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
    18841 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
    18901 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
    18961 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
    19021 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
    19081 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
    19141 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
    19201 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
    19261 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
    19321 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
    19381 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
    19441 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
    19501 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
    19561 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
    19621 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
    19681 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
    19741 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
    19801 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
    19861 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
    19921 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
    19981 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
    20041 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
    20101 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
    20161 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
    20221 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
    20281 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
    20341 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
    20401 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
    20461 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
    20521 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
    20581 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
    20641 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
    20701 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
    20761 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
    20821 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
    20881 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
    20941 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
    21001 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
    21061 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
    21121 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
    21181 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
    21241 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
    21301 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
    21361 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
    21421 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
    21481 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
    21541 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
    21601 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
    21661 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
    21721 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
    21781 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
    21841 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
    21901 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
    21961 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
    22021 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
    22081 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
    22141 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
    22201 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
    22261 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
    22321 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
    22381 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
    22441 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
    22501 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
    22561 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
    22621 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
    22681 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
    22741 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
    22801 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
    22861 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
    22921 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
    22981 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
    23041 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
    23101 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
    23161 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
    23221 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
    23281 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
    23341 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
    23401 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
    23461 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
    23521 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
    23581 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
    23641 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
    23701 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
    23761 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
    23821 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
    23881 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
    23941 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
    24001 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
    24061 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
    24121 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
    24181 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
    24241 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
    24301 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
    24361 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
    24421 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
    24481 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
    24541 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
    24601 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
    24661 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
    24721 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
    24781 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
    24841 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
    24901 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
    24961 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
    25021 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
    25081 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
    25141 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
    25201 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
    25261 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
    25321 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
    25381 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
    25441 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
    25501 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
    25561 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
    25621 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
    25681 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
    25741 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
    25801 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
    25861 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
    25921 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
    25981 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
    26041 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
    26101 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
    26161 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
    26221 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
    26281 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
    26341 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
    26401 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
    26461 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
    26521 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
    26581 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
    26641 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
    26701 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
    26761 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
    26821 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
    26881 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
    26941 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
    27001 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
    27061 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
    27121 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
    27181 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
    27241 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
    27301 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
    27361 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
    27421 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
    27481 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
    27541 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
    27601 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
    27661 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
    27721 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
    27781 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
    27841 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
    27901 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
    27961 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
    28021 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
    28081 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
    28141 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
    28201 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
    28261 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
    28321 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
    28381 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
    28441 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
    28501 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
    28561 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
    28621 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
    28681 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
    28741 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
    28801 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
    28861 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
    28921 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
    28981 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
    29041 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
    29101 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
    29161 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
    29221 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
    29281 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
    29341 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
    29401 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
    29461 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
    29521 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
    29581 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
    29641 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
    29701 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
    29761 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
    29821 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
    29881 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
    29941 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
    30001 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
    30061 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
    30121 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
    30181 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
    30241 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
    30301 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
    30361 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
    30421 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
    30481 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
    30541 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
    30601 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    30661 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    30721 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    30781 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    30841 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    30901 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    30961 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    31021 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    31081 ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa
    31141 aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa
    31201 agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt
    31261 tcctgatctt tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa
    31321 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
    31381 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
    31441 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
    31501 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
    31561 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
    31621 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
    31681 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
    31741 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
    31801 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
    31861 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
    31921 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
    31981 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
    32041 ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac
    32101 cgtgtacc
//
LOCUS       DACELF010000024        31740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.024, whole genome shotgun sequence.
ACCESSION   DACELF010000024 DACELF010000000
VERSION     DACELF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.024"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            363..731
                     /locus_tag="IW687_001062"
     CDS             363..731
                     /locus_tag="IW687_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0950338.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IW687_001063"
     CDS             911..1852
                     /locus_tag="IW687_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0950339.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IW687_001064"
     CDS             1921..2652
                     /locus_tag="IW687_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0950340.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IW687_001065"
     CDS             2796..4976
                     /locus_tag="IW687_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0950341.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IW687_001066"
     CDS             4996..5478
                     /locus_tag="IW687_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0950342.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IW687_001067"
     CDS             5494..6255
                     /locus_tag="IW687_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0950343.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IW687_001068"
     CDS             6386..7720
                     /locus_tag="IW687_001068"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0950344.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IW687_001069"
     CDS             8027..8512
                     /locus_tag="IW687_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0950345.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IW687_001070"
     CDS             8505..9188
                     /locus_tag="IW687_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0950346.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IW687_001071"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IW687_001071"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0950347.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IW687_001072"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IW687_001072"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0950348.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IW687_001073"
     CDS             12804..13433
                     /locus_tag="IW687_001073"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0950349.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IW687_001074"
     CDS             13485..13817
                     /locus_tag="IW687_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0950350.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IW687_001075"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IW687_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0950351.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IW687_001076"
     CDS             14643..16016
                     /locus_tag="IW687_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0950352.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IW687_001077"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IW687_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0950353.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IW687_001078"
     CDS             17071..17511
                     /locus_tag="IW687_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0950354.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IW687_001079"
     CDS             17655..18356
                     /locus_tag="IW687_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0950355.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IW687_001080"
     CDS             18353..19474
                     /locus_tag="IW687_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0950356.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IW687_001081"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IW687_001081"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0950357.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IW687_001082"
     CDS             20853..21761
                     /locus_tag="IW687_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0950358.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IW687_001083"
     CDS             21796..22068
                     /locus_tag="IW687_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0950359.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IW687_001084"
     CDS             22079..23125
                     /locus_tag="IW687_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0950360.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IW687_001085"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IW687_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0950361.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IW687_001086"
     CDS             complement(26136..26807)
                     /locus_tag="IW687_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950362.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IW687_001087"
     CDS             complement(26800..27294)
                     /locus_tag="IW687_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0950363.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IW687_001088"
     CDS             complement(27314..28534)
                     /locus_tag="IW687_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0950364.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IW687_001089"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IW687_001089"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0950365.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31737)
                     /locus_tag="IW687_001090"
     CDS             complement(30610..31737)
                     /locus_tag="IW687_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0950366.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10566 a         5667 c         6793 g         8714 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaaccatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatcttcgc cgtttgtata attaagcgag acaaataaaa aagccattta
//
LOCUS       DACELF010000025        31661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.025, whole genome shotgun sequence.
ACCESSION   DACELF010000025 DACELF010000000
VERSION     DACELF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.025"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..344
                     /locus_tag="IW687_001091"
     CDS             <1..344
                     /locus_tag="IW687_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950367.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="IW687_001092"
     CDS             425..1045
                     /locus_tag="IW687_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950368.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1375..2601)
                     /locus_tag="IW687_001093"
     CDS             complement(1375..2601)
                     /locus_tag="IW687_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0950369.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2673..2831)
                     /locus_tag="IW687_001094"
     CDS             complement(2673..2831)
                     /locus_tag="IW687_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950370.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3519..4259
                     /locus_tag="IW687_001095"
     CDS             3519..4259
                     /locus_tag="IW687_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950371.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4351..5538)
                     /locus_tag="IW687_001096"
     CDS             complement(4351..5538)
                     /locus_tag="IW687_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0950372.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5860..6702
                     /locus_tag="IW687_001097"
     CDS             5860..6702
                     /locus_tag="IW687_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0950373.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6699..7649
                     /locus_tag="IW687_001098"
     CDS             6699..7649
                     /locus_tag="IW687_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950374.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7664..7897
                     /locus_tag="IW687_001099"
     CDS             7664..7897
                     /locus_tag="IW687_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0950375.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8228..8812
                     /locus_tag="IW687_001100"
     CDS             8228..8812
                     /locus_tag="IW687_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0950376.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8943..9611
                     /locus_tag="IW687_001101"
     CDS             8943..9611
                     /locus_tag="IW687_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0950377.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9613..11037
                     /locus_tag="IW687_001102"
     CDS             9613..11037
                     /locus_tag="IW687_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0950378.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            11055..11624
                     /locus_tag="IW687_001103"
     CDS             11055..11624
                     /locus_tag="IW687_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0950379.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11734..13488
                     /locus_tag="IW687_001104"
     CDS             11734..13488
                     /locus_tag="IW687_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950380.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13442..15214
                     /locus_tag="IW687_001105"
     CDS             13442..15214
                     /locus_tag="IW687_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950381.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            15737..16006
                     /gene="rpsN"
                     /locus_tag="IW687_001106"
     CDS             15737..16006
                     /gene="rpsN"
                     /locus_tag="IW687_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0950382.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16021..16200
                     /gene="rpmF"
                     /locus_tag="IW687_001107"
     CDS             16021..16200
                     /gene="rpmF"
                     /locus_tag="IW687_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0950383.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16232..16609
                     /locus_tag="IW687_001108"
     CDS             16232..16609
                     /locus_tag="IW687_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950384.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16630..16779
                     /gene="rpmG"
                     /locus_tag="IW687_001109"
     CDS             16630..16779
                     /gene="rpmG"
                     /locus_tag="IW687_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0950385.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16803..17786
                     /locus_tag="IW687_001110"
     CDS             16803..17786
                     /locus_tag="IW687_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0950386.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17816..17938
                     /locus_tag="IW687_001111"
     CDS             17816..17938
                     /locus_tag="IW687_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0950387.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            17956..19479
                     /locus_tag="IW687_001112"
     CDS             17956..19479
                     /locus_tag="IW687_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0950388.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19503..19742
                     /locus_tag="IW687_001113"
     CDS             19503..19742
                     /locus_tag="IW687_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950389.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(19935..20396)
                     /locus_tag="IW687_001114"
     CDS             complement(19935..20396)
                     /locus_tag="IW687_001114"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0950390.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20591..20809)
                     /locus_tag="IW687_001115"
     CDS             complement(20591..20809)
                     /locus_tag="IW687_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950391.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            20927..22543
                     /locus_tag="IW687_001116"
     CDS             20927..22543
                     /locus_tag="IW687_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0950392.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(22784..24124)
                     /locus_tag="IW687_001117"
                     /pseudo
     CDS             complement(22784..24124)
                     /locus_tag="IW687_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(24400..24555)
                     /locus_tag="IW687_001118"
     CDS             complement(24400..24555)
                     /locus_tag="IW687_001118"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0950393.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(24851..25183)
                     /locus_tag="IW687_001119"
     CDS             complement(24851..25183)
                     /locus_tag="IW687_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0950394.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(25697..25888)
                     /locus_tag="IW687_001120"
     CDS             complement(25697..25888)
                     /locus_tag="IW687_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950395.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(25982..26368)
                     /locus_tag="IW687_001121"
     CDS             complement(25982..26368)
                     /locus_tag="IW687_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0950396.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            27062..28267
                     /locus_tag="IW687_001122"
     CDS             27062..28267
                     /locus_tag="IW687_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0950397.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            28284..28718
                     /locus_tag="IW687_001123"
     CDS             28284..28718
                     /locus_tag="IW687_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950398.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            29335..>31661
                     /gene="esp"
                     /locus_tag="IW687_001124"
     CDS             29335..>31661
                     /gene="esp"
                     /locus_tag="IW687_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0950399.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT        10867 a         4615 c         5823 g        10356 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagctggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gaaattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaaattt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtctcaaa aaggctcgta
     1261 aagcatctca attctctaaa cgttaatatt atttatatat caatgtttca aggcactttt
     1321 caattgtttg aaaagtgtct ttttcttgtt taagaacaca tttaagaaca catactaatc
     1381 acatttattc aatcctctct gaaaggcttc aaaaacagtt ttatctacta catctggtgt
     1441 atttacgtat attctcgttg tagaaatatc tgagtgtgtt aaagcactag atatttgatt
     1501 catatttgct ccgccttgtc tagctatagt tgcataggta tgacgaagct tatgtggaga
     1561 taaatgagcc aattcaggat gtcgtctttt aattgaattg attcgatagt ttaggtaatc
     1621 agcgtgaaca gggcaattta cttctccgga tggtttagta taggtaaaga gatactgatc
     1681 tggtgtttga ttaattttta atctcaacaa ttgctcagct tgcactgatt tccatttgct
     1741 tagtaatgcc attacaattt cgggtacttg aattacggta tcctttcgac cttttgtaaa
     1801 tttctttttt tgatatttat ccaaagcatg tttaaggcga accgtttgtt tctcaaagtc
     1861 gatatatttc cattgcaaag catacgtctc actttttcta tcaccaagat atagggtaag
     1921 catgaaaaga gtatagtcat gaagagtaag caagtgatta ttcaagtcag tgtttactgc
     1981 ttctatccaa gcaataagct cttctgacga taatgcttga ttaccttccc gtatccttct
     2041 ttcttctaaa gcaatttttt taggtgcagt gatacattga ataattttgg tagttcggtt
     2101 aaattcaata taatttagta tttctgcaat atcaaacata gaacggacat atcctttgat
     2161 aatttttata tttgcgtatt ctttcgattt ttttgttaat tcatctgata ttatctcagt
     2221 atttgaattt agatagttca ttgcatactc accaaacatt ggaaggatgt gcaatcggaa
     2281 tatatcttta gtattactaa ttgttacgta acttggagct ctttttgatc tagtagtctg
     2341 acctagttca tatctaggaa gccattttga ttcatagaat tccttaaatt ttatttttcc
     2401 ttttaattct agtgaatttg cattctcctc tcttaaaact gtttcaattt tccttttcat
     2461 tgcattttcg gctttaatag ccaattgcct agttggaaat actttttgat acttttctga
     2521 tgtaattcca gtgatttctc tgaatttttt tggatacctc agattagttt tatatttccc
     2581 atttgaaagt tttgtaggca aaattcctca ctcccttaaa aagtaaggca gtatatttcg
     2641 ctattcttag tatatgcggc gagaagaaat tttcaaattg ttttaaatgt attcataaaa
     2701 tctttgatat cctctatgtc aaagcgaact ataccgtcaa ttctgattac tggaaaatta
     2761 tatatacgaa tccacttatc taaagtatta ttatcaattc ctagaaactc acaggtttgt
     2821 tttttattca agtagactct caattttata aaatcatctc ctaaaaaaga ttagaatttt
     2881 tgaattgctt tgggtaagca cataggaatt tcacctctcc ccagtttacg gcgagcctta
     2941 cggaagtatc attatttgct gctattgtca tggcattttg ttttcccaga acaaaaagaa
     3001 atctctaagt atttgtcgct aatcaggtca tggcgtactt gagtttctag ctcgaatagc
     3061 agggatcgtt attcaaaaat tgtatttaat tgtcaatgat caaaaatagg ttttttccta
     3121 ttcactatta ataacgataa acatgtataa aaagacacac tacgaaaaaa gttaaatgga
     3181 gtttactgat tttcatattt gtatatggaa cagacatagt cttgaataat ttttataaat
     3241 gttttgatgc ttatcaactt attcaacccg ctatccactt taagtataat ttttccatac
     3301 gccaataaag tagttttagg agcatgttct catttgagaa cgctcctttt ttatatattt
     3361 tgaaagctac ttactatacg cgtagtttca aatagttttg tcattctaat cttcagcaac
     3421 agaaaaaaat ctaaattata aaatttaggg ttgactaaaa tataattttt gtataccata
     3481 tgatcgtgat aatcattatc atttagaagg agaaagttat gttagaagtg aaaaagctta
     3541 atgtttgtta taacgatttt ttagcattga acgagatttc tttaaaaata caagaaggtg
     3601 ctttaacagg tattattggt ccaaatggtg ctggaaaatc tacattgctt aaagctatgt
     3661 taaatattat ccctcatcag ggagaggtct ccattaataa tgaagatatc aataaaaaac
     3721 tggtgaagat tgcatatgta gagcaaaaag ctgatattga ttttactttt ccaataaaag
     3781 taaaagaatg tgtatctatg ggaacatatg ccggaatgaa agtttttcaa agaattaaaa
     3841 atgcagaatg gcaaagagtt tcaaaggctt tggaaaaggt cgatatggga aaatattcta
     3901 atcatcaaat aggagaatta tctggaggac aatttcaacg tgtactttta gcaagatgtc
     3961 ttgctcaaaa tgcggatttc atatttttag atgaaccatt tgtagggata gacttagtta
     4021 gtgaacgaat aattatggat acgttaaaag aattgaaaca tcagggaaaa acagtgctaa
     4081 ttgtgcatca tgatttaagc aaggtaaaga agtattttga taatattatt cttttgaatc
     4141 gaaatctaat tgctcacgga agtgtagaat ctgtatttaa tgaagagaat ttaaaaaaag
     4201 cctatggaga tactatattt attggagaga ctaactatat agagtcaagt aaaaattagc
     4261 caaatcaaaa aatattttat aacagataat tccgaaaaaa gggtacaaca aataagcttc
     4321 ttaccattag tttttttgaa tgttcaatag ctaatcagaa acaattatat aaggctcgtt
     4381 tttagtcaaa atagcatgaa tcacgtaaca aagcttacga gaaaccgcac ctaaggcagt
     4441 tagatgatgt tttccttcgg aaactttttt ctggtaaaac gcactaaaaa caggatcaca
     4501 ttgagaagct actaaagccg ccgaaaacaa ggcttttcgc aaatatggag accctcgttt
     4561 actcataaca ttatgcgttg cctcatattg ccctgattgt gtcacggaag cgtctatccc
     4621 agcgtaagca acgagttttt tcgggttaga aaatttttga atatctccga tctcaccaag
     4681 aatagtcgca ccgtttatag gaccaattcc aggaattgtg agaataacag aatttaaagt
     4741 gtccattatg tgcttgattt ctctttccgt ttcctttatt tggctttcaa tgaattttaa
     4801 ttgttcaatc attgaacgta gttggaaggt gaacgcttct tgagcaaatt ttactccaaa
     4861 ggagttagaa gctgcttgct tcaattggtc cgctttttct gccttgattc ggttcctgct
     4921 taactcagct aagtattggg ccaaagtttc tgaggaaatg ttttctaagt cgatcggggc
     4981 actaaacgcc agcaataact ctttagaagc ttttccgaaa acgccctgct tggtaaaaat
     5041 cgtcgcgtat tctggaaaga cttgatccaa tacggctata atttttcttt tgaagtcgct
     5101 cgctgtacct actaaataag ttcggtaacg agaaagttgt ttcaaagaaa aaacattttc
     5161 atcggataag accgtttcga caaatgagcc atagcgcatt aagtcagcaa tcaaaacaga
     5221 atcaataata tcgttttttc gtttccgtat ttctgtacct ttacgccatc cgtctgtttg
     5281 aaggggattc acaacgtgaa tcaaataatc tgattcgtga aggtatgaaa agatggacag
     5341 ccaatagtgc cccgtggctt ccatccctat aacaaagtca ctgggtgctc cggaatagtc
     5401 gaccattcgc tcaattaatg actctgctcc ttcagtggtg ttaggaaatg aaaaaccttt
     5461 tagtaatacc tttccttcct cgttcatgat agaagcaacg tgagtgcgtt taccgatatc
     5521 aatgcctaaa aagtacatgt atatctgacc tttcaaaaaa agaattttgg attgaaatcc
     5581 actcaactga aaagcgtcac taccttgtac gagatacgga gtaaagtaag gactttcaac
     5641 atctaactca tacgcaatcc acttatcaga gagaggagcc agtctctcga gtgcgggttc
     5701 aatggcccaa ggggggaacg gcttagcctc tcaatccaat aaatatatta tctcaaaaga
     5761 aatgagttaa taccaaatta aaataacgat ggttcatagg gttatccaat caaaaccgga
     5821 aggttaaaac cctaactcta atatacaagg gggaataata tgcttcaaaa ttttattaat
     5881 ggtctgtatg actttcactt tttacagaat gctctaatta catctgtagt tatagggatt
     5941 gtttctggaa cagtaggttg ttttattatt ttaagaggaa tgtctcttat gggtgatgct
     6001 atttctcatg ctgttttgcc aggagtagca atttcttata ttttaggtat taattttttt
     6061 attggtgcga ttgtatttgg tctattagct tctatcatta ttacttttat aaaaagcaat
     6121 agtgtaataa aaggagatac agcaattggt attactttca gttctttttt agcattagga
     6181 gttatcctta taggagttgc aaatagctca acggatttat ttcatatctt atttggaaat
     6241 attttagctg tacaagatat tgataagtgg attacaattg gcgtttctgt tttagtgttg
     6301 gttacagttg ttctattttt taaagaatta ttaatcactt cttttgatcc attaatggca
     6361 aaagcaatgg ggatgaatgt cagtttttat cattatctat taatgattct attaacttta
     6421 gtagcagtaa cagctatgca aagtgtggga actatattaa ttgttgcaat gttaattaca
     6481 ccagctgcta cagcatattt atatgcaaac agcttaaaaa ctatgatatt tatttcggcg
     6541 acagtcggag cgtgctcatc attgctaggt ttgttcattg gttatagttt taacatagca
     6601 gcaggatcaa gtattgtttt aacagcggca ttcttatttg taattagttt tctaatctca
     6661 ccaaaacaaa aatttttaag aaagaaggag agaaagttat gatgagaaaa tggaaagcag
     6721 tactgggaag tctggggatt ttgattgctc tttttatatt cggggcatgt tcaacaaata
     6781 gtaaagacaa agatacagtg gcttcgaacg aaaaattaaa ggtagtagtt actaattcga
     6841 ttttagcaga tattactgaa aatatagcaa aagataaaat tgatttacac agtatcgtac
     6901 ctattgggaa agatccccac gaatatgaac ctttgcctga agatgttcaa aaaacttcaa
     6961 aagcagattt gattttttat aacggtgtta acttggaaac tggaggaaat gcttggttta
     7021 caaaattagt aaaaaatgcg aacaaagagg aaaacaaaga ctattttgca gcaagtgatg
     7081 gcatagatgt tatttactta gagggtcaga gtgagaaagg gaaggaagat ccccatgctt
     7141 ggttaaattt agaaaacggt attatttacg ctaaaaatat tgaaaaacag ttagcggaaa
     7201 aagatcctga taataaaaaa ttctataaag aaaatctaga taagtatatt gaaaaattgg
     7261 attctctaga caaagaagct aaatctaaat ttgcttcaat tccgaatgat aaaaaaatga
     7321 ttgttacaag tgaaggatgc tttaaatatt tctcgaaagc gtataatgtg ccttctgctt
     7381 acatttggga aatcaacact gaagaagaag gaacaccaga tcaaataaaa cacttagttg
     7441 aaaaattacg cacaacaaaa gttccctcct tattcgtaga aagcagtgtg gacgatagac
     7501 caatgaaaac agtatcaaaa gataccaata ttcctatcta ttcaacgatt tttactgatt
     7561 caattgcaga aaaaggacaa gatggtgata gttactatgc gatgatgaaa tggaacctgg
     7621 ataaaattgc tgaaggcctt tcgaaataat ttggagggta ggtatggtac aaattaaaac
     7681 gactactata ctaatttatt tactagcaat tcaggaatta tcaaaaagaa aaaagaaatt
     7741 aaaaaataat gatttagcaa aagttctaag tgttagtcca gcatctgtaa gtgagatgct
     7801 ggctaaactt agatatgagg actatttaga tataggattt caattaactg ataaagggaa
     7861 aaaatttatt gacgattaca aaaaaggttt taagtaaaag actttatttt tagggaggat
     7921 aagaaaatgg ctattggagt gattatgata ggacttaccc taatagctgg atataatttt
     7981 tttaaatctt tctcggatta ttttagaaaa ggtgaatgat ttagatgagt ctattaattc
     8041 ttgtaggatt gctttttgta tcagttattt tgctctttct cattagttta gctctaagga
     8101 atgagttcag taatagacgt taacgattga aacttcaagt cctgtgtaag ataatatagt
     8161 taattcatct tattaggagg aagtacccat agaaaccaga gattatatta caactcacca
     8221 aaaattaata gaaagtggac taagccattt tgaaaaagaa ggatttcagc aaactaagat
     8281 aaggcaactg tgcttgaccg caggcgtaac tacgggtgct ttttataaac attttacttc
     8341 taaggaagcg ttatttgaag aaattattca gccgcatatt aatgcgctta ttaatatgta
     8401 taatgaagag tttaccaaat acctcttaaa cttagaaaat atagatatag agaaaagttg
     8461 gagagagaac ttcaatgaat tagatccatt tattaattat atttttgaga ataaacgtat
     8521 attcgatttg gttttattcc aatcagctgg aaccaaatat caagatattg ttgaaaaaat
     8581 tacatcattt gtgacaaaaa agacagtctg ttgtctagat aagttaaaga ctatcggaat
     8641 gatcaatcaa aatttagtct ttaatgaaga agaaatccac ttctacattt actcattcta
     8701 cgctacattc tatgatatat tgaagcatag ctattcaaaa gaaaagacta tgctattaac
     8761 cagaagattg tatcaatttt ctgttcctgg gtggattgaa ttactgagtt aattaaaaaa
     8821 aagcttgcat tgagaattgt tctcagttat gctttttgat aagatcgcac tgttatctga
     8881 ataacggtgc atttttgtca gcttaagata acagtgcgat cttatttttt ggaggtgaaa
     8941 gagtgtcaaa aatcaatccg ttaaccttga ttactttaaa tatttttttt ccggtcatta
     9001 tttttttagg aaagggaaat atatttcaag tggggtgttt cattattgct acagttttag
     9061 tattgatgtt tcgtaaaata aagacatgta tttatttcat cttgatttat tcagttctgt
     9121 ttttagtaat gaaaattatt gatgctatca agatagagtt ttttactata ttttttggga
     9181 catccttata tattgtattt cgaatgttgc cggtcgtaat gatctcatgg ttgttggtta
     9241 gtgtctatac tagcaatgaa ttattatcct ctttagaaaa aatacattta ccgaaaaaaa
     9301 taatgttaag tattacagta gtgatcagat tctttccgac ttatcgttct gaaataagaa
     9361 tgattaaaga atctttaaaa atgagaaaca ttcgcttgtc tttttttcag cctctgcaat
     9421 atttggaatt tctgttagtt cccgttctta tacgtgcaac tatgattgct gaagaaatga
     9481 ccgctaatgc aataacaaag gggatagaat ctcctgtgaa aagaagttct tactataaaa
     9541 caaacatgag cttattagac tatatcatcc tgttttccgt tactattttt ttcttaagta
     9601 ttttgatttg aggtgaaaga attgtttgaa tttaacaatg tttctttttc atataaggat
     9661 gttaaggaac aagcgcttgt tgaccttaat ttttcaataa aaaaaggcag tcttacattg
     9721 attactggtt cttctgggag tgggaaatcc acactattaa agttaatgaa tggacttatt
     9781 ccaagacttt atgacggaaa attggttggg gaaattttat ttgaaaataa ctcgctacaa
     9841 tccttaacac aagaaagtat atcaaaaaat attggctacg taagtcaaaa tcctagagga
     9901 caattcttta caacaaatac aacgagtgag ttagtatttt cgatggaaaa ctttggattt
     9961 acaaaagagc atatggataa aagaatcaat gaattagctg aattattaga tctttcaaat
    10021 atattagata aaaacatttt ttcgctatca agtggtgaac ggcagaaaat ttctattgga
    10081 tgtagtttat cgctaaactc taaattaatt atattggatg aaccgtcttc aaatttggac
    10141 ttcaaagcga ctaagaaact tgctgactta ctttgtgatt taaaaaataa aggatttact
    10201 attgttatag ctgaacatcg tttttactat ttgaaagaat taattgatca agttctattt
    10261 gttaaaaata gaactgtaaa aactacaact attcagttgt taaaaactga aaacaataac
    10321 gcattaagga aatttgacat ttttaatagt tcgattgaag ttccctatat cgaaaaggta
    10381 agtgcagatg tattaaaagt tgaagaaatt acttatgaaa atattctaaa agacataaat
    10441 tttgatgtca aagttggaga tgttattggt ttggtaggga aaaacggtgt ggggaaaaca
    10501 accttactaa gaatactcat gaagattatt cgccctacca aaggaaagat aatcgaaaat
    10561 aaaaaaaatt tgtcatcgcc gtttttagta atgcaagaaa tggattacca atttttcaca
    10621 gaatcagtga ggagcgagtt agccctgggg aatgaaatag ttagtaaaga tcagcaagaa
    10681 aaaattttaa aaaaaatgga actttataaa atgaaagatc aattaccgtt tgatttatcc
    10741 ggtggagaaa aacaaagatt attggtttct atagcttcat tgtcaaaaac taatttattt
    10801 ctatttgatg aacctacaag tgggttggat tatatcaata tggaaaggat tgctgacctt
    10861 attaaagact taaaagaaaa gggtgcagta gttatagcca ctcatgatcc ggaatttctt
    10921 tataaaacat gcaatagaat tatttattta aaaaatggtg gaataaaaaa agatataaaa
    10981 cttagagtag aagattcaac tttgatagaa cagattttta atgacataac gaaatagaat
    11041 tgaggagaat atatatgaaa acaaaagatt atatttttat tggtttagct acagttattt
    11101 ccctagtaat ttactttttt tcaataatgg ttagtagtat tggtggggcg tttggtcaca
    11161 gcatatcccc agggattttt ggattattat ctggaatcat atttgtttat attagctata
    11221 actaccctag aaaaggaatt tttactattt acacaattgt tttattgtta ttttttacgt
    11281 taatgggcgg ggcgtactta ccttggttta tcagttcgat tagtatggcg atcttagctg
    11341 atattattct atctgtattt ggatatgatc gagcaattcc tcaagttgtg tcttgggttc
    11401 taatgcaact aggcagtgct gccggacagt ggattcccat atggttcttt acagatcgtt
    11461 ttcgtcaaga ctggatagat agaggacaat ctgctgcaac aatggatgca atgatccact
    11521 acgcggtggg tatatgggga attatttcag tgttagttgt agcaagttta tctatgattg
    11581 gtgtattgat tggaagaaaa gtcttaaaaa aatacaaaaa atagaacgta gtttaccttc
    11641 tatctgtttg tacatctcta atttacgcta ttacggaaag cattaacagt tgtaaacgaa
    11701 taagagaaga aattgaatgc gggggaggaa aaaatgaaag tatacaaaaa attatttagt
    11761 tatgtcccta aaatgaaata ctacggatgg ttagccactt tattttctga tttttctgtc
    11821 gtattaacag tctatggata ttacagtatt tacaaatttc ttcagtctct aattattgta
    11881 ggagatgaat atttagctaa gagttatgcg gttcaaactg ttgcttggtt aactttagga
    11941 gctttatttt atattttttc tggcttattt tctcatatcc ttgggtttcg cttagagact
    12001 aatttgagaa aaaagggaat tagcggttta acagaagcaa gttttcgttt ttttgattta
    12061 aattcttcgg gatatattcg aaagacaatt gatgacaacg cggctaaaac acatatggca
    12121 attgctcact tgattccaga tagtgctcaa gccatagtaa cacctgtttt atcagttgcc
    12181 ttaggctttt tcattaactt taaaattgga ttggttctaa tcgccatgtt agtaattggt
    12241 gtattcttgt taaagaaaat gacagagaat acaaatttta ttcaaaaata tcaagaatcg
    12301 ttagatgtgt taagctctga aacggtagaa tatatccgcg gaattcaagt tataaagata
    12361 tttggtgcta aagtaacatc atttaaagcc ttgaataaag caattaacga ttatgctaag
    12421 tttgcatata agtattcttt gagtgggaaa acaccttttg ttctttttca atggctattt
    12481 tttggcatag tttgcatttt gatagttcct ataacctttt ttataacttc tcttaccact
    12541 cccaagatct tagctgttga cttgttgatg ttgttttttt taagtggaat tatttttgtt
    12601 tctttcatga gaataatgta tgtaagtatg cacttgtttg aagcaagata tgcagtcgaa
    12661 acactagact ccctttattt agaaatgaag caagaccaac taaaatatgg gaacgagaca
    12721 cagattaaaa attgtgacat tgcatttgaa aatgtcagct tttcatacaa agaagagtgt
    12781 gttttagaaa atttcaactt agttttagaa caaaaaagaa tttatgcatt agtaggaaaa
    12841 tcaggaagcg gaaagagtac gatagctaaa ctaatttcag gtttctacaa tgtggataag
    12901 ggacagatta aaattggtgg taagccttta aatcactatt caaaagacac aattattaac
    12961 acaattgctt ttgtctttca agatacgaaa ctattcaatc agagtattta cgataacgtt
    13021 tctatagcaa atcctcaagc gccagcaagt aaagtatttg acgcgctaag attagccggt
    13081 tgcgaatcga ttttagaaaa atttcccgaa agagagcata ccttaattgg tagtaaagga
    13141 gtttatctgt caggaggaga aaaacaacgt atagctattg ccagagcaat tttaaaagat
    13201 gcaaagattg ttattatgga tgaagccagt gcatctattg atccagataa tgagtatgaa
    13261 ctacaaaagg cttttcaata tttgatgaaa gaaaaaacag ttattatgat agctcatcga
    13321 ttatccagca ttaaatctgt agatgaaatt cttgtcttgt cagaaggaaa aatagtagaa
    13381 cgtggaaatc ataatgaatt aatggctaaa catcgagtgt ataaacaatt ggtcactaat
    13441 tatgagaatg caaatgattg gaggcttgac aatgaaaaat ttttataaaa aaaaatttgc
    13501 tttaactgat cagggcgcag aagctttaac taaagcttct atttctagtt tttttgtata
    13561 ctgcattaat atggtaccag cattcattat aatgatgctg atagacgaat tagttttaga
    13621 aaatgcaaag ccccgttggc tttattttgc agtatcgttt gttactttgc ttttcatgta
    13681 ttggttgtta gatagggagt atgaaaactt atataatagt acttataaag aaagtgcgca
    13741 tttaagagtg caaattgcag acgatttgtc aaatttacca ttatcctatt tttcaaaaca
    13801 taatttatca gatttatctc aaactatcat gtctgacgtt gaaggtattg agcatgcgat
    13861 gagtcatgca atacctaaag ccggcggtat ggctctgttt ttccctttta tttcagtgat
    13921 gcttttggtt ggtaatgtca aaatgggatt agctgttatt ttgccaacgt tatttagttt
    13981 tgtcttaatc ttgttatcaa agaaatccca aacgaaagcc aatactaaat attacgatac
    14041 tttgagagaa aactcggaag aatttcaaga aactattgaa ttgcagcaag agattaatag
    14101 ctttaatcta tctaaaaaag ttcaagacag acttttcaaa aaaatggaag agagtgaaag
    14161 gattcattta aaggtagaat taagtacttt ttcagtcatg gccttatcct ctattttctc
    14221 atatgttagt ttagcagtag tcattctagt aggtgttcac ttactgttaa cgggtgaagt
    14281 gactatactc tacgtcgttg gttacttact agccgcaata aaaataaagg attccttcga
    14341 ttctatgaaa gaagcagtgc ttgaaatatt ttatttagct cctaaaatac agcgaattag
    14401 agctatgaag gaaaccagta tccaagaggg atcagatagt cctctgaaat cttttgacat
    14461 tgagttgcga gatgtctctt tctcatatga taacaatacc ccaattttag atcatatctc
    14521 ttttacggct aaacaaggag aagtgactgc gttagtaggt gctagtggtt ccgggaaaac
    14581 tagtatcttg aaattagtgt ctagactgta tgattatgat gaaggctgta ttttaattga
    14641 tgggtatgat attaaacgtg tatctccagc ttctttgttt agcaaaatcg ctattgtttt
    14701 tcaagaggta actttgttta atacaagtat tttggaaaat atacgtattg gaaatagtca
    14761 agcgtcagat gaggaagtga aaaaagcagc tcgtttagct aactgcgaag actttattga
    14821 aaaactacct gacggttatc atacattggt cggagaaaac ggaagttcac tttctggagg
    14881 agagcgacaa cgcctatcca tagcacgggc ttttttgaaa aatgcgccaa ttttaatttt
    14941 agatgaaatt actgccagtt tagatgcgga aaatgaaaaa cgtattcagg aaagtttaaa
    15001 tcgtttaata caagataaga cagtactaat tatatcgcat cgcttgaagt ctattgaaaa
    15061 ggtaaataaa attgtagtga tggatcaagg aaaactagtt gatcaaggta ctcataatga
    15121 gttgtatcga aggtcagaaa tatacaaaaa tctcataaag aaaactaaac tatctgagaa
    15181 attcgtctat gaaaaagaag ctcaatcacg atgatgaaat aggctttaaa ttaacctaca
    15241 atttttggga tatagttata attctttttc aaaaagaata attttattaa acagcatcta
    15301 attttgaata cacgaaagaa tcagtatgat tgaattagtt cacttaatgt ctattcttat
    15361 aatataagga ataatcaaag ttttaaaaaa ttggcgataa accagttaaa cataaaagat
    15421 tgaataaaaa gttatagttt ctttcatatt gccgttttaa gtagaggaaa agctacttaa
    15481 aacggtattt gtgtttgatt ggactaataa aagtgaattc ttatttttca gagcctaaac
    15541 tgcatattct tttaaatgat ttgattatct ctatgtaccg ataaattcgt tttcaaatag
    15601 ttatctattc atatgagttt tatttcatgt tatttattgt tgacaagagc aactcttgtt
    15661 ggtaaagtat cagtgtcaaa acgtaacgat tacgatttga ggtgtctaaa tcaaaataaa
    15721 aggagtataa aggtatatgg caaaaaagtc gaaaattgct aaagcaaaga aacaaatggc
    15781 gatgattgaa aagtatgcag ataaacgtca ggaattaaaa gccgcaggag atcgcaccgc
    15841 tctcgctaaa ttgccaagag attcaaatcc gaatcgtctg aggcttcgtg atcagacgga
    15901 tggtcgtcca agagggtata tgcgaaagtt cggtatgtcc cgtattaagt ttagagaact
    15961 tgcccatcaa gggttgattc ctggagtaaa aaaagcaagt tggtaaaagg aggaaatatc
    16021 atggcagtac cagcaagaaa aacatctaag gcgaagaaac ggttaagaag aactcatcaa
    16081 actgtagtaa aaccagaaat ttcatttgac gaagcgaatg gtgattatag acgtagccat
    16141 catgtatctt taaaagggta ctataatgga aagaaagtaa ttaaaggagc ttcaaaatag
    16201 tttaatatat aacttttagg agggaatcaa aatggaaatt atttacccac ctttagttga
    16261 agaaggatta aagtattatc ttgaaacgac ccagcaatcg ctagataaat gcacttttta
    16321 tcgttcgatg gtagagagag gaattattac tgaaacgggt ttaccgaccc aacaggcaat
    16381 tgaaaatgga ttagtcaaag attactatga ggaccaaggc ttatcatttg atgatttttt
    16441 aagaatctat ccaatttttg aagagtatga cgaggagtta tttcaatgta tcgatggtta
    16501 ttgggagata cctgttgata tgaaagaaaa tttagtttca caattggaat ctggcgagtt
    16561 aacttttgaa gatgcacaac aaattcaagc atatcttgaa gatcgataag tattgaagga
    16621 ggaagattta tgcgtcaaaa tattattttg gaatgtgtcg aaactggaga acgtctgtat
    16681 ctcaccagca aaaataaacg gaataaccct gaacggttag aattaaaaaa gtattcgcca
    16741 aaattgcgca gacgggcaat ttttaaagaa gtgaaatagc taagtggtaa aaaggtgaca
    16801 aaatgggcat accagttaca atcgtttctg ggtttttagg ggctggaaaa acaacgctaa
    16861 tcaatcaagc cttgcaagtc tcaccctacc ccagggaaga aattattata attgaaaatg
    16921 agtttggtga agttgggatt gatcacaaat tattagttca tagcgaggaa cgggtgttac
    16981 aactaaataa tggatgtatg tgttgcagtc ttcgcagtga tttattagca atattgattt
    17041 ctttactgga aacaatagaa gaacatcaac aaccaatttc acaaatcatc attgaaacta
    17101 caggtatagc agatccccaa ccaatcattc aaacactttt aacagcgcct caactaagag
    17161 gaagagtata tatcgatagt ttattgactg ttattgatag tgacagtttt gaacttattg
    17221 aaagagaacc acaagccctt aagcaacttg tcatggcaga tcgcattttt gtgtctagaa
    17281 aacaaaactt gaatttatct gcactaaaaa aattacagag agaaatcaaa agtattaacc
    17341 ctttatccga tattcgaatg ttcacacata gtgaaacgat tcaaaaaaag gatttttata
    17401 atttgaaaaa atttaatcat cccttagttt ttgaaagtga ttcatcagaa aatcactata
    17461 atggcaaata ctatcatcaa caccatcaag tacatgagtt taaatcaatt ctcttaactt
    17521 cagatcaaga cataaaaaaa gattttttac tccaatggat tgactggctg attttaacca
    17581 accaagggag cctgtatcgt tttaaaggat tgattgcttt acaagagcag gattttatag
    17641 ttgccatgca agaggtaaac gaacaggtta attttcaata caccactcga caaaaagacc
    17701 aaaccactat tattctaatc ggaaaaatgt tggatgaaga aaggatcaaa cagagttttg
    17761 ataaattact ggcggaaagc aaataatgat aagtagtgat tgggggtaat ttctaatgga
    17821 gaaaacggat atatctagtg catatcgacg acttaaaagc ccgaatatta aaactagaaa
    17881 aagggcatta aaaattatca aagaacataa acgaaataaa atgaagaaat tagcataatt
    17941 aaggaaggaa aaagaatgaa gaaaatcagt gtaggactta ttgggatagc agctctagga
    18001 ttattgggag cttgctccag cactaatgat gcaaaagtat cgaatgataa agatggaaaa
    18061 ttagaaattg taacaacatt ctatccaatg tatgatttca ccaaaaacat cgtcggcgat
    18121 gaagcgaacg tggatttaat ggttccagcg ggatcagaac ctcatgacta tgagccctca
    18181 gctaaggata tggcaaaagc tcatgatgct gatgtttttg tatatcataa cgaaaacatg
    18241 gaatcatggg taccaaaagc taaagaaagt tggaagaaag ctgggcctaa tgtagtagaa
    18301 ggaacaaaag acatgatttt attgccaggt agtgaagaag aagatcatga tcacggagaa
    18361 gaagatcacc atcatgaact agatcctcat acgtgggttt cacccaaaat ggcaattaag
    18421 gaagtttcaa atatcaagga tcagttagtt aaattgtatc cgaaaaaagc aaaagtattt
    18481 gagacaaatg ctgaaaagta tttgactaaa ctgaaacggt tggatgctga ctataccact
    18541 agtcttaaag aagcgaaaca aaaaagtttt gtgacgcaac acgcagcctt tggatattta
    18601 gctttggatt atggattgaa acaagttcct attgctggac taagtcctga agaagagcca
    18661 tcttctggtc gattagctga attaaaagag tatgttaaaa aaaataagat caattatatt
    18721 tactttgaaa agaatgctaa tgataaaatt gcaaaaactt tggccaacga agctgggata
    18781 aaattggaag tactgaatcc tcttgaaagt cttacaaaag aacaaatgga taatggtgaa
    18841 gattatgttt cagtaatgga agacaatcta aaagcgttag aaaaaacaac tatggttgct
    18901 ggtgaagagg tagttccaga aaaagaagcg aaggatgaaa agacagttgc taatgggtac
    18961 tttaaagatg ccgatgtgaa ggatcgcgaa ttgagtgatt atacaggcga atggcaatct
    19021 gtttatccgt tgttaaaaga tggaattttg gatgaagttt tcgattacaa agcaaaaatc
    19081 aataaagaca tgacagctgc cgagtacaaa gattattata ctactggcta taagacagat
    19141 attgatacta tcaacattaa agacaacacc attgattttg tagtcaacgg tgaacatcat
    19201 caatacacat acaaatatgt tggatacaaa attttgaatt atgaaaaagg aaaccgtggt
    19261 gttcgtttta attttgaaac tgatgatgca ggtgctggtc gttttaaata tattcaattt
    19321 agtgatcatg gaattgcacc aagtaaggca gagcatttcc atattttctt tggtggagaa
    19381 agtcaagaaa aactctataa tgaattgcat aattggccta ctttctatcc agcttcattg
    19441 tctaaacatg aaattgctca agaaatgatg gctcattaaa attcaatttt tggaggaaat
    19501 acatggtaag aaaagtatta ttgtctatgg attccaagaa tattgatgct tatcggcgtt
    19561 atcgatttgc aaagcaaatg agattaaatg ggagtacatt agctgatatt gctgcaactt
    19621 tacatgttag tattgacaca gttcatagag tattaaaacg ggatcctgat tgttgtttta
    19681 tcaaggtcaa tactggaaga agtagacgtt tagcaaaacg gcataatagc ttggccaatt
    19741 aattgataat accagagtag atcatttttc ccttatttgg atttgatctg ctttttatta
    19801 tgtcttgaat tgtcaaatca agcgcaacac tttatcaaag aagacttcat aaggtgttct
    19861 ccaatctaaa cactttctag gtctgttatt cagtttttca atcatctgat ttatcgttga
    19921 gttatctacc gtagctatct ctttcgattt tggcaagtat tctcgaatga gtctattcgt
    19981 attttaattt gttcattgtt gccaagggca tgaggatctg gaaaataaaa gggcatatta
    20041 ttcaatgctt ctgtaactgc gaaatgattg gagaattctt ttcctctatc tggagtgatc
    20101 gttttgaccg cctcatttgg aatactgctt aaaagttcaa tcattttctc gctcacataa
    20161 gcggctttct ttctggggac cttctttcct gctaaaagga agcatgattt tctgtctgtc
    20221 aaagtcacta aacaagtacc accagttttg ccagctacaa tatcggcttt ccaatgacca
    20281 aaatcacttc tatcatctgc ggattttgga cggccatgga tcgaatgagt aaggcaaatc
    20341 tttccacgag actcgacatg attttttgta tgtcttgttt ttcctcgatg gtgcaatgaa
    20401 cggatcaggc ctttattgcc gtgtgagagg ggttctgtat cgaaaagatc attgcaaatt
    20461 gcacgttaga tcgtgataaa actaattgaa aagtccatat gttcatgctt caaacgattc
    20521 gagatttcct ctggcgacca ctgaaaatcg acaaataaat gacgaacaat tgaccaaact
    20581 cacggatcat ttagaatgtg ataacgtccg catttttttc ttcgataagc ataatttttt
    20641 tccgctttgg aaggtgaata gtttttttct tgtttcttct cagttcatgt aaaatagtaa
    20701 acgggctatg ctgaatgtcg ttgccaatta tactaataga aaatccttct tcgtgaagca
    20761 gaaagattct ttctcgttcg agtatagtaa gatgacgata ctggctcata attattttcc
    20821 ttctaggagt gttctcgaca aacttatact agttggatat cgattatgac tcatttttat
    20881 gtgttgcact taaagtgtaa attcaaggta tagaaagagg ttccctatga aaaaaatacc
    20941 tattacaatc ttaactggct atcttggttc aggaaaaacg atacttctaa atcatattct
    21001 gtacggtgat cacggaaagg aaattgctgt cattgtaaat gacatagggg aagtaaatgt
    21061 tgatggggta ttagtcgaca agaaaggttt taaaagaact ggagaaaaac ttgtttcgat
    21121 gccgaatgga tgcatttgtt gtacattacg cgaagatttg attgttgaac tagatcaatt
    21181 agtgcaattt tctgacttga atcaaattgt tattgaagct tcaggaatca gcaaaccaat
    21241 tccaattatt caagcaatta tattagctaa aaacccttta gacattgatt tgacccaacg
    21301 attagagatt ggtgccatta ttatagtagt ggatgtaaaa cgtttttggg ataatttcga
    21361 aacgggtgaa aagattcagg aaagaaaagc tgaaggtaat gtagttgaag aagaacagga
    21421 tattcgtggc ttgttaattg atcaattcga gtgttgtaac atattacttt taaataatag
    21481 tgacttagtg tcaaccaaga cgatttacaa aattaacggt ctaattgcta agttacagtc
    21541 caatgccaaa ataattagga caattaaagg tgaagtagac ttatcgaaaa ttctaaatac
    21601 aaatcttttt aatatagatg agacttccta ttcagtgggc tggttcaaag agttagagct
    21661 tggctatcaa aatcatattc ctgaaacaga agaatatggt atctcaagtt ttatctatcg
    21721 aaatcggtta ctccttgacg cacaaaaatt tgcccatctt acggataaaa attttcctgg
    21781 caatattaca agagcaaaag ggtttctttg gtttgcccaa catagtgatt tttcgacgat
    21841 ctttgaaaca tcgggtagct ataaaagtat tgaatcatta aattattggg tagctagttt
    21901 acctaaaatt gagtgtcaaa tgattataca aaaaaatccc gaattgaagg aagcctggca
    21961 tccgttgttt ggtgaacgca ttaataaact agtttttatt ggtatgaaga tggatcaaca
    22021 atgtattaag gaacaacttg agggttgttt aactgattct cttaatgcag ataatttccg
    22081 aaaaatttca ccatttccat ggaaaaagtc taatatgttt agccagatat cgttaatgaa
    22141 atttttaaga aaagctggtg tcaagatagg cgatgtcata attattggca ttttgattat
    22201 tacctctttt attcccttat ttgtattctc tatagaaaag gagaatgaaa caactgaaga
    22261 tgctatcaaa tatgccatcg ttcgaattga tggcaaggaa atggatcgct tcgatttgga
    22321 taagatcaat cataaactcg tgaattatca tcctgcgaag ggacaatata atattgttga
    22381 gattaaagat ggaagaattc gagttaaaga agataatagt cctgatcaaa tcgcggttag
    22441 aactggatgg atctcaaaac ccggtcaaac gagtatttgt ttaccccata agttagttat
    22501 tagtattgag aaaaaagaaa gcacagatta ctacatttac taatctggaa atagatgagg
    22561 aggtttttaa taagaaaaag atattattat taataatagc aggggcttta ttaagtgtgt
    22621 taagcgcttg tggacaagaa aaagaagcgg tgacaacagg cacaagtatc tttaacggga
    22681 cctgcaatgg tttagttaac agtcggttag attacttatt ttggctcttc gtcaatgagt
    22741 gttggtgaag aaattcaata gaataatcga gatactcaca cggctagaca gctatcttgt
    22801 ctagccgtgt tgttgttctg gctttagtca gttgggtagt attcttagtt ttcttaaaat
    22861 attttcctcg atataaaaag attcgttctc ggtagttttg aaagtttcta tagccatagg
    22921 caattcgttt tagtactttg atatggttat tcaaacattc tatagggccg ttggaatagg
    22981 gaacgcgcaa cgctcgtttg atttctgttt ggtatttctt gaaggtcgtg aacactggtt
    23041 ggtagtgctc tggcaactcc ttgtagtctt cttttaggag cgcgacgaat tcaggaacat
    23101 cttgtcttct gataacggaa agaaaatcct ggtagacttc gtagcctcgc tttaaggaat
    23161 catcataaga taatagccga tcgactatct ctgcttctgt cagatgatct cgaaatgacg
    23221 gacgccagta gcgatgctga cctttcaatt cccacgcatt cttttgaagt agtttccagt
    23281 atttctttaa gtgcttcgac cgacgatccc cttttttaaa ggtcttcatt acttgaattc
    23341 ggtggttggt aaaagcccga ccaatgtgtt ggctaatatg aaagcgatcg gcgacgattc
    23401 gggcattcgg aaaacactct ttcaccaatg tgcgataggg tgtatagaaa tctgaaacca
    23461 ctagacgcac ttgttcacgg acttttatag ggtagcgcaa gaaatagtcg cgtaaaggat
    23521 tgagttgccg attttccaca atatcaatca gttgatgggt cttgccgtcc atcatgacaa
    23581 agctcatggc tcccgagaca tttttcactg atttgaattc atcgaaacac accacttctg
    23641 gtaacagctg gctcaaagaa atggttttag gccgaaggct gcgaagtatt cgaatgacag
    23701 tggtcggaga aatgtgcttc atacgtgcga tcaaagacat agaaagaggt tcgcttaata
    23761 gctcaagaat ggcttgtttg actctgcgag caatggagca gcgacgatca ctaactgaat
    23821 cttctgctaa aaaagtcttt ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca
    23881 gaatggtctt gtagttactg acatcattca ataagatgga tacttttttc cagccctatt
    23941 taatgatcgt ttgttcattt tgatagtggc gatgaaagca ttcctccggt tgataggtca
    24001 atgtgccatc aaaaacaaaa catgtttcgt cattgattcg ctctttgcga aaggcatctt
    24061 catgaaagat aagatttagg tctagaatat ctagtaactc tttggtaaac tctgaatagg
    24121 acatcttgaa acactccttt gaatggtttt cgtcgacttt attctaaagg attttaaggt
    24181 gtctgtttgc gtttattaga aaaaagaaaa agtattggca acagaaggcg tcaccaacac
    24241 tagatattat agatccctta ttttcttcgc ttcaaattgt caaggagtca tcataaattt
    24301 tgtatggatt cttttgtttg ggtagtagtc ttctaaataa tcttggatta acaccttcat
    24361 ctctatttta cttttatatt tgttggagtc gattaattct catttgattg atcgatgaaa
    24421 cgcttcaatt actgcattgt caacaggagt cccagtttgg ctcactgaat gtgtgatgtg
    24481 gtggtcgttc aacagctctt gataaataaa agaccgatgc tggctacctc tatcggaatg
    24541 aattaccgta ggcaattctc cttaataaat ttgtaatgga cggacgacaa tctctttttt
    24601 catgtgtgaa tctaccatat agtaacgtag aatacgagtt gctaagtcaa tataggtgga
    24661 caaatagagg cgaccgtcat tacatggaat gtaagtaata tccatcaccc aaacagcatc
    24721 aatttggttc tgttcaaatt atcgattcaa cattttttta ccatttcttt gattgttttt
    24781 tctctaccat gagaagattt gcgtcgatga aatcctcttg tgcataaatt catttcacgc
    24841 ataagaacag ctacaacacg tttattgatt tcgattcctt catcgtaaag tttttgagtg
    24901 atccctggag atccgtatcg atctttgtgt tcgtagaaca ctcgtcttcc tcttttagtc
    24961 gtttattttc ctgttcaaga atgtagaaat ctgctttatc aggtaatctt tttccttgtc
    25021 cagcaaaagc atttttcccg tatcgacgat attctgaaac ccatcgggaa attgttggac
    25081 gagagatatt gtattcttca gcaattttct tggtagttac ttttttctca aggatatcct
    25141 gtgaaatctt tagtttaaat ttacgatcat atgtttttct caatagagtt cattccttcc
    25201 ggtaacgtaa ctgatagtca agaaaagtgt atcatagccc aacttagtat cagggtttgt
    25261 caactaaact gtggaagttg atagataaga gtttttaaca accaaaattc tcttgactat
    25321 ttttgaaatc agacaaattt tgattggaca catagttgaa taaatctacc gtcgttacgg
    25381 aaggtaaatc gcatgctttt caattctaga ggagaagtcc tcgttgaagt accatagccc
    25441 agttctggat tcgacctcct gcccacttca tacgtaaata aagtaatttt aggagtgaga
    25501 ctgtgacaaa agttattttt aagctaaaaa cgaattaaaa aacgttgata attaaaatca
    25561 ttcgttttag attctatgaa tcattataga acgctgtaaa atcgatactt tataattata
    25621 aagaagatag tttcaaattt attgttcgaa atttgaaact atctttttac ttatgtcaca
    25681 gactccctta gttgagttat tctttaattt cgaatttcga gatagctaga tatattgcaa
    25741 gcatacattc ttgataacaa tcttgatcat attttccagt tctccaggat tgcttatgaa
    25801 tcaaagagct aaatcttttg agaattgcct caacagcttc ttcatttcca gcctttgcta
    25861 aaatgaaatc ttcttttaaa gaattcataa atctaccttc ttcccatgga cttataatct
    25921 atttctttgg gaaggaggag aacgagcaaa gaagcataca aaattagtta ttatgcagat
    25981 attaaaccag cattttttga atttttttgt aaagtcgatg ttttaaattt gtcgcaccct
    26041 gtcttgtaat ccccagaacc tttccaattt ctttatcagt tttatcaaag ataaattttt
    26101 cgataagaaa aatttgttca ggttctttca acaaaggaag gatttttgct aattttatat
    26161 cttcaacaag gagagtcaca tttaaaacat taatcggttg tttaaatttt atagtatctt
    26221 tagtaaacaa atatggtggg agttgatcaa gttgttcatg tttttgaatt tcttcttgtt
    26281 ttttataata agtattagcg gtgttcaaaa tgaccttttc aaagaatcga atcaactggg
    26341 catttacgcg ttcgtcgtta tctgacatat tgcacacctc ctccattaag atttaaaggg
    26401 aaaagaggca aaaaacacga aaagaagcta atgcaattat gctgcgctgc gaataacttc
    26461 tataagctca catgcattga taggactgct gatgtttatt ttcaaaaatg tttttttagt
    26521 tgataatgga tagttatctt tatacttaga aaagaaaatt catatatctt cttaaatatc
    26581 aaacatgtcc ttatggctaa gaaaatgtct tgagcttttt acgtataata aatcttttct
    26641 aaaacctcct tttggataat attcttattc gtgtatagat tgtatttttt atttcttccc
    26701 ccttcataat taacaactag aaaagagttc gaatagacac gtatttgaac gaaaattttt
    26761 attggattcc caaattggaa tatttagaaa cgaaaaattc ctattatctg tatagtgctt
    26821 agataataac ggatcataat aattattgtc tttgaactag atatatgtaa atagtattca
    26881 tcatgatgaa aaatgatgag ttctcggtga taaaatgtac tttttacggt ggaatataca
    26941 tgaaagcgat aagtggatat tttataatga agtaagttag cttattttga cagaggaata
    27001 gtattttaga taacgtataa aatactattt aatgatgtag aaaggaaaga gagaaacaaa
    27061 agtgttttca gataaagaag tcttattaaa gtctcttcat gtttgtttaa agttacctat
    27121 cgtggttttt aattccgaat tcagtattat aaaagagtat cgctcagata gaactactta
    27181 ttttttttat gattataaaa agttactaac tgataatatt aaaaaaacta aagatttcca
    27241 tttctttact ggtgatttta atgagatgtt tcttctatac atgtttaaaa ccagatatta
    27301 cttatttggt ccatttagag caaataatat tgataaagtt ttttttaaac taaaaatgaa
    27361 caatttgaat gttgcaatgg ctgatagaga acgactctat aattcattgc aaaatttgac
    27421 tttgtattct ttaggagaca ttcgagatat tctgatttta gttcattatt tttttactgg
    27481 aaaaatagaa gatctattcc atgaaccgtt aattgaatat acagaaaatc taagtaaaac
    27541 tatcgagcaa atcaaaatag ataatctgtt atcacaaaat tacgatccag aaatatatct
    27601 gtttctttat gaaaataaaa tactggaata tgttaaaaat ggagatattc gaaatttaga
    27661 aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag atacgattcg
    27721 gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt ctataacatt
    27781 aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag aaaacgaatt
    27841 agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt attatactaa
    27901 ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg tcgttcaatt
    27961 catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt atttttctgt
    28021 aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga tttataatta
    28081 tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc atactatcag
    28141 tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag tattcaaaaa
    28201 gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt tcaacaacat
    28261 tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg gaggagttga
    28321 ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt tgtttcgtag
    28381 aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac aaagtagtaa
    28441 ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtcat attaatggta
    28501 gcaattttaa acaaacatta tttaatctga ttaagctaga tgctaaaata tcaagttatt
    28561 tattcttttt gatacatgat gaatttatgg attatcaaaa attggatcag ttaattgaaa
    28621 gagaaagctg ggaagattat tacgtgggtc taactttttc agaaaagtta aacaactaca
    28681 agttaataga ttataagtat ttgagtgaaa gcaattaatt attcatgtag agtactagac
    28741 actttaaata tggacaattc aattataaag aaaaagaatc aataatttaa tgaggctgtg
    28801 acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa ctccaatcat
    28861 tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt ataattataa
    28921 agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact tatgtcacag
    28981 tctcattcag taatgcggga taaggctcct tattccgtta tttgaatact ctgaagttta
    29041 atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc atagaaatga acttaatttc
    29101 agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt aaaaagcaca
    29161 aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgttca tgaaattctc
    29221 taagtaaatt agttaacata aggttagtaa gattcctatt ctgtgaataa tacgtatcag
    29281 atttatcttt tattcttact aggaaagtaa ttattaaggg agttgatttg aataatggtt
    29341 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
    29401 atcaaaaaat taagtgttgg ggttgcatct gttttgattg gtgttggtct tgtcttggga
    29461 acaactgaac tagttaaagc acaagatgaa atttctccaa gcagcccact tgaaacagcg
    29521 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
    29581 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
    29641 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
    29701 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
    29761 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
    29821 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
    29881 ggattatttg ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg ggtaattacg
    29941 tttgaaaaga gcctagatga aatttttaaa gagataggca tagacaatct caagaccgat
    30001 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccgggt
    30061 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
    30121 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgagaaacct
    30181 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
    30241 cataagttaa cgaaaaatta taatttctca tattcatctt ccgcaaaggg cacgccttgg
    30301 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
    30361 atggttcagg ctgacaaagt aacatatgat gtttcgtttg aatccggaac aaaagtagct
    30421 gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gcataggttc aataactaat
    30481 aatgatttaa ctaaacttat cgattttgct aatgcaagtc cacgtccagt cgttattcga
    30541 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
    30601 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
    30661 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
    30721 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
    30781 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
    30841 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
    30901 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
    30961 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
    31021 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
    31081 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
    31141 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
    31201 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
    31261 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
    31321 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
    31381 ccagatgttc ctttagctaa agatgccttt tcaatttcag atgtctataa taaggacttc
    31441 ggtaatgcaa gtgttgatgc aaacactggt attgttacat tcactccagc taaaggtgta
    31501 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
    31561 gtaggcacga ccgatttagc agtaactgta agtaaagata tttatgaaaa tccaggagaa
    31621 aacattcctg caggctacca caaagtaacc ttcaccgcag g
//
LOCUS       DACELF010000026        31131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.026, whole genome shotgun sequence.
ACCESSION   DACELF010000026 DACELF010000000
VERSION     DACELF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.026"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     regulatory      292..389
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            505..1086
                     /locus_tag="IW687_001125"
     CDS             505..1086
                     /locus_tag="IW687_001125"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0950400.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            1089..2396
                     /locus_tag="IW687_001126"
     CDS             1089..2396
                     /locus_tag="IW687_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0950401.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2812..3300
                     /gene="purE"
                     /locus_tag="IW687_001127"
     CDS             2812..3300
                     /gene="purE"
                     /locus_tag="IW687_001127"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0950402.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3284..4417
                     /gene="purK"
                     /locus_tag="IW687_001128"
     CDS             3284..4417
                     /gene="purK"
                     /locus_tag="IW687_001128"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0950403.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4430..5725
                     /gene="purB"
                     /locus_tag="IW687_001129"
     CDS             4430..5725
                     /gene="purB"
                     /locus_tag="IW687_001129"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0950404.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5905..6282)
                     /locus_tag="IW687_001130"
     CDS             complement(5905..6282)
                     /locus_tag="IW687_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950405.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6538..7266
                     /locus_tag="IW687_001131"
     CDS             6538..7266
                     /locus_tag="IW687_001131"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0950406.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            7266..7520
                     /gene="purS"
                     /locus_tag="IW687_001132"
     CDS             7266..7520
                     /gene="purS"
                     /locus_tag="IW687_001132"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0950407.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7522..8193
                     /gene="purQ"
                     /locus_tag="IW687_001133"
     CDS             7522..8193
                     /gene="purQ"
                     /locus_tag="IW687_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0950408.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            8194..10416
                     /gene="purL"
                     /locus_tag="IW687_001134"
     CDS             8194..10416
                     /gene="purL"
                     /locus_tag="IW687_001134"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0950409.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10401..11840
                     /locus_tag="IW687_001135"
     CDS             10401..11840
                     /locus_tag="IW687_001135"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0950410.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11872..12915
                     /locus_tag="IW687_001136"
     CDS             11872..12915
                     /locus_tag="IW687_001136"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0950411.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12912..13490
                     /gene="purN"
                     /locus_tag="IW687_001137"
     CDS             12912..13490
                     /gene="purN"
                     /locus_tag="IW687_001137"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0950412.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13487..15022
                     /gene="purH"
                     /locus_tag="IW687_001138"
     CDS             13487..15022
                     /gene="purH"
                     /locus_tag="IW687_001138"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0950413.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            15038..16312
                     /gene="purD"
                     /locus_tag="IW687_001139"
     CDS             15038..16312
                     /gene="purD"
                     /locus_tag="IW687_001139"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0950414.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16434..17348
                     /locus_tag="IW687_001140"
     CDS             16434..17348
                     /locus_tag="IW687_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0950415.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDGLERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17481..19046)
                     /locus_tag="IW687_001141"
     CDS             complement(17481..19046)
                     /locus_tag="IW687_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0950416.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(19039..20490)
                     /locus_tag="IW687_001142"
     CDS             complement(19039..20490)
                     /locus_tag="IW687_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0950417.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20504..21520)
                     /locus_tag="IW687_001143"
     CDS             complement(20504..21520)
                     /locus_tag="IW687_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950418.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21740..22828
                     /locus_tag="IW687_001144"
     CDS             21740..22828
                     /locus_tag="IW687_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950419.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22882..24408
                     /locus_tag="IW687_001145"
     CDS             22882..24408
                     /locus_tag="IW687_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950420.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24410..25615
                     /locus_tag="IW687_001146"
     CDS             24410..25615
                     /locus_tag="IW687_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950421.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25872..26849
                     /gene="guaC"
                     /locus_tag="IW687_001147"
     CDS             25872..26849
                     /gene="guaC"
                     /locus_tag="IW687_001147"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0950422.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            27113..28537
                     /locus_tag="IW687_001148"
     CDS             27113..28537
                     /locus_tag="IW687_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0950423.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28998..30107
                     /gene="pdhA"
                     /locus_tag="IW687_001149"
     CDS             28998..30107
                     /gene="pdhA"
                     /locus_tag="IW687_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0950424.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            30110..31087
                     /locus_tag="IW687_001150"
     CDS             30110..31087
                     /locus_tag="IW687_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0950425.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        10013 a         5295 c         7024 g         8799 t
ORIGIN      
        1 ttgaacacta tttattcgga aaaaggggct atgacatgct ttttgtcatg ccttcttttt
       61 atgtgggctg cttttttaga taggagaaaa taaaaataac tatttaaacc cgaacaaaaa
      121 ggaataatgc attaaaaatc attagatttt acaaaaaaat aagtaaacca cgttgtcagt
      181 ttcagaaagt agtgttagac tgttttcaat atttgaaaca acatttttta ttcaaatatg
      241 gcaaactgcc ttcacgtgca aaacagttgc aagaaaatga atttacacta ttattagttt
      301 tacataccta cttatatatc gtcataatat ggatgacagt ttctagccag taccgtaaat
      361 gctggactat aagtaaaaga ttggctattt cacccttctt ttcttatgat aaagtggagg
      421 gtgtttttct ttaacagaaa catagtaggt ttttgcacaa aagcggttat ttcggtatta
      481 ataaatgata aaggagcaaa taaagtgaaa gaattagttg aacgtattca aaaagacgga
      541 cgtatattag gcgagggcgt attaaaagtc gacagcttcg tcactcacca agtagaccct
      601 gtattgatgg aacaaatggg aaaacggttt gcagaagtat ttaaagaaca aggaatcacg
      661 aaagttgtga ccattgaagc ttctgggatc gcaccagctt tatttgctgc aagagaatta
      721 gatgttccaa tgatcttcgc tagaaaagca aaaagtttga cgatggatga agaattattg
      781 acagcttctg tttattcttt tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa
      841 tttttatctt cagaagacaa agtattgatt atcgacgatt tcttggcaaa cggacaagcg
      901 gcaaaaggat tgatcgaatt atgccagcaa gctggagcgc aagtagaagg aatcgggatc
      961 gtcatcgaaa agtctttcca agatggacgt cagttacttg aagaaatggg attgagagtc
     1021 gtttctcttg cgcgtattgc ttccttgacg aatggacaag ttgagtttct tgaggaggac
     1081 gcataatcgt ggaaaaagaa acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc
     1141 tgctagcgat gtatgcagga gcagtagcgg tgcctctttt gatcggtaca ggactaggct
     1201 ttaatcaaga acagatgact tatttgattt caattgatat ctttatgtgt ggattagcga
     1261 cattattgca attgacagtt aaccgatttt tcggaattgg cttgcctgtc gttttaggat
     1321 gtgcgatcca agcagtagct ccactgatct tgatcggaac caatgaagga gtaggagcta
     1381 tctacggatc tattatcgct tctgggatct ttgttgtgtt agtttctggt gtcttttcta
     1441 aaattaaaaa actattccct cctatagtaa caggaacggt tatcaccgtt attggtttga
     1501 ctttgattcc tgtagcaatc gagaaaatgg gtggaggtaa tgcaacagca gctgattttg
     1561 gtgacaaaac aaatctgcta cttgcatttg tgacgattct cctaattgta ggcgtgcaaa
     1621 tgctggcaaa agggttcatt cgttcaatcg cggtattgat tggtttagtc ggaggaagca
     1681 tcttagctgc tttcatggga atgattgatc tgagcgcgat tggagatgcg ccgttgttcc
     1741 atgttccaca acccttttat tttggtaaac caactttcga cgtatggtcg attttactaa
     1801 tgatcatcat ttccatggtc agcatggtag aatcaacagg tgtatatttt gcacttggtg
     1861 acattactgg gaaaaatgtt ggagaagaag aattgaaaaa gggatatcgt gcagaaggac
     1921 ttgccgttat cttaggcggt attttcaata cattccctta tactggcttt tctcaaaatg
     1981 tcggattggt tcaactttcg ggaatcaaaa caagaaaacc catctatttc tctgcattct
     2041 tcttgattgt tttaggatta ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc
     2101 cggtacttgg tggaggaatg ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta
     2161 tgctgttgga agttgatttc agcaatgata aaaatctatt gattgttgct gtttctgttg
     2221 gatttgggct gggtttcaat gttacgccga cattattcag tcaaatgccg caaacattgc
     2281 aaatgttcac tggaaatggc atcgtaatga gtagcattac ggctatcata ttgaatttga
     2341 tctttaatgg attgaaaaaa gatgaagaaa tattggaaga agccttcaag aagtaataga
     2401 gcaaaagaaa ggttgtgaaa tcagcgtttt gacctgattg ttagagaaac aaacggaaaa
     2461 atagttcctc gtattttgcc gtttgtttga gctaataccg agggtcagct gattgaacac
     2521 cgttgaagga agaggtcgtg agcctgccgt tcatctccga gtcacaagga acaatttcaa
     2581 aaaaaagctt ctcatgtttg ttgaatattg ttccttgtga ccgaggagtt ggcaggagaa
     2641 caccgtttat ccataaaaag gaatgtgacc taacttttgt cacattcctt ttttttatct
     2701 ttaataaaca cgaacaataa aatgtttttc tttgttaaca ttcgaaattt ctttgacatc
     2761 aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg gaggaaaaac aatgacagca
     2821 aaaatctctg tgattatggg gagtacttca gattgggaaa caatgaaaga agcatgtcaa
     2881 atattggatg aattcggtgt gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca
     2941 gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag gaatcaaaat cattattgca
     3001 ggagcagggg gagccgcgca tctgccgggt atggtcgcat caaaaacaac tttgccggtt
     3061 atcggtgtac cggtccaatc acgaacatta aatggtttgg attcgcttct ttcgattgtc
     3121 caaatgcctg gaggagtacc tgtagctact acagcaatcg gaaaagcggg agcaattaat
     3181 gctggactac ttgcgataca gatgctttcc atgtatgatt tagaattgga ggctaaattg
     3241 gctgaaagac gttcaatgtt ggctaaaact gttatagaaa gtagtgatca acttggttaa
     3301 acctttgttg cctggttcaa cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat
     3361 ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg ctggatcctg tgagcgattg
     3421 tccagccgca caagttgcag attggcacat tgaaagtgtc tatgatgaca cctttgcgct
     3481 gaaagagctt gccagaagaa cagatgtcat tacctatgaa ttcgaaaacg tcagtataga
     3541 ggcgttaaat gcgattttac ccatgtcatt tattcctcaa gggacagact tgcttgcgat
     3601 tacgcaagac cgcctgttag aaaaatcttt tttggaaacg aataacatcg tgattgctcc
     3661 ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg attgacggga tcggctatcc
     3721 ttgtgttttg aaaaccactc gaggcggcta tgatggcaag gggcagtatg ttttgaaaag
     3781 tcgggcggat ctagctcctg ccatggacct tttaagagaa ggaacctgtg ttttagaagc
     3841 atggatcccg tttgaaaaag agatttcgat catggtggca ggaaatggtc aaggagattt
     3901 cacgactttt ccggtcgtgg aaaacatcca ccacaacaat attcttcatg aaacgattgc
     3961 tccagccgcg attgatcagg atgtgatcga ggaggctgag cgtattgccc gtgtgattgc
     4021 agaagcagtg agcctttcag gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg
     4081 gctatatgtg aatgaattag ctcctcgtcc tcacaattct ggacattatt ctattgaagc
     4141 ttgttctatg agtcagttcg atgcccatat tcgaggtatt tgtggttggc cattaggaga
     4201 aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt ttgggagaac aactgctgga
     4261 aagctatcgc ttgattgaga ataagccaga ctggcaattc catttctatg gaaaaaaaca
     4321 agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg acagaagaca tccagcaaac
     4381 gctggaagaa atcaaacaaa cgaatatttg ggattagagg agaaaaaaca tgttagaacg
     4441 ttatacacgc cctgaaatgg ggaatatctg gacggaagag aacaaatacc gtgcgtggct
     4501 cgaagtcgag attttagcag atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga
     4561 tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa
     4621 attaacacgc catgatgtcg ttgcgtttac acgagcggtt tctgaaacat tgggagaaga
     4681 aagaaaatgg gtccattatg gtctaacaag tacggatgtc gtagatactg cttatggcta
     4741 tctcatcaag caagcaaatg atattttacg ggaagacttg aaacgtttta cggaaatcat
     4801 tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg ggtcggacac acggtgtcca
     4861 tgctgaacca actacttttg gattgaaact tgccacatgg tattcagaaa tgaaacggaa
     4921 catcgaacga tttgaacatg cggcaaaagg agtagaagca ggaaaaatct caggagcagt
     4981 gggaacgttc gcaaatatcc cgccatttat tgaagagtat gtatgtaaac aattggggat
     5041 ccgtccacaa gaaatttcta ctcaagttct ccctcgggat ctacatgctg aatatttctc
     5101 agcgatggcg cttattgcta caagtattga acgttttgca acagagatac gcggattgca
     5161 aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc
     5221 tatgccgcac aaaaggaatc cgatcggttc agaaaatatg gctggtctag ccagagtggt
     5281 gcgcggacac atggtcactg cctatgaaaa tgtcacatta tggcatgaaa gagatatttc
     5341 tcattcttca gcagaacgaa tcatcttgcc tgataccacg attttattga attacatgtt
     5401 aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa
     5461 tatgtctgca acatttggtt tgatttatag ccagcgcgtt atgctgaaat tgatcgataa
     5521 gggaatgaca agagaagaag cttatgatct tgttcagcca aaaactgcgt acgcttggga
     5581 caaccaagtg cagttccgcc cgctgttaga agaagatcca accatcacat cggtattatc
     5641 taaagaagaa atagacgatg cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat
     5701 attcaaacga gtaggtttgg aataaagaag agtgagttgc aaaattcaat taaaatagaa
     5761 tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt
     5821 atattttctt atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct
     5881 ctttacatac aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg
     5941 ctggttatgg agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt
     6001 gaataagatc tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat
     6061 tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca
     6121 gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa
     6181 ataaacgtct ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa
     6241 ttctgataaa aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga
     6301 acccattatc caaattttta gttaattcta atataccatc attaagtata aatgtaaacg
     6361 cttgttgatg gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg
     6421 ccttaaatgt acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa
     6481 aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg
     6541 gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag
     6601 aacttgattt ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac
     6661 cagataagtg gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg
     6721 actcaaagag gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca
     6781 aaaaaagtac aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc
     6841 tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac
     6901 tataaggatg accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa
     6961 cttgcaaagc cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt
     7021 ttgaccgaat tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc
     7081 cggtcgaatg gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg
     7141 gatgaacgta cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg
     7201 atcccagtct atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga
     7261 gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag
     7321 gggaggcagt aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa
     7381 taggaaaata ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg
     7441 aaacgatttg tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca
     7501 aaaaaatgga ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt
     7561 gacgcagata tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg
     7621 catgatgagt cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat
     7681 ggtgactatt tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt
     7741 cgttttgctg aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca
     7801 gaagcaggac ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa
     7861 actgtaccat tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa
     7921 ataatctatc ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt
     7981 caagaactaa aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc
     8041 gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat
     8101 ccagaacgtg cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc
     8161 attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct
     8221 gaagagatca agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga
     8281 atgatcgaag aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct
     8341 gttatgtgga gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct
     8401 actagtggac ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt
     8461 gatggactag cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct
     8521 tatgaaggag ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct
     8581 cgtccaattg cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa
     8641 tatttactag aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg
     8701 actgtaggcg gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca
     8761 atgtgcgtag gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt
     8821 aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt
     8881 gcctcagaag aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat
     8941 ccttttatgg aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat
     9001 tctctaatag gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg
     9061 gcttcaaaag ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact
     9121 gggatgacac cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt
     9181 aaatcaggag aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc
     9241 actatcggta aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta
     9301 gcaaatctgc cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa
     9361 gaaccagctc gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa
     9421 cctggacaag tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc
     9481 tacgaaactt atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct
     9541 gctgtgatga gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg
     9601 cgctatctgt atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga
     9661 aatattattg ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct
     9721 cctgataaac cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg
     9781 tgtgaaacgt taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat
     9841 ggtcaagcaa tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa
     9901 catatcacta ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg
     9961 tttgcagatt ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg
    10021 atccgaaact ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc
    10081 caaaacggat tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg
    10141 gcagaatcag ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct
    10201 caattatttg cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca
    10261 cgattcgaag aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg
    10321 ataatcgaaa tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag
    10381 tgctgggagg atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga
    10441 tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc
    10501 ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg
    10561 ggcatcgaga tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt
    10621 taacaggaga agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg
    10681 acaatattca gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg
    10741 gtaacttgat caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc
    10801 attctaactc cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt
    10861 ttttaaataa attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa
    10921 tgacagaaac ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg
    10981 gacaaatgaa aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg
    11041 gggcacgttt tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa
    11101 tccagattga ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct
    11161 attttgccag accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata
    11221 tgggacggaa tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta
    11281 attcttcttt gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg
    11341 gattagtaaa aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag
    11401 agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga
    11461 ttatggtcga tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga
    11521 aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat
    11581 gtttttacgg gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg
    11641 atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga
    11701 tcgaatcgat cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca
    11761 acggagatta tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag
    11821 aaaaagtatc gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat
    11881 gcatacgcaa aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag
    11941 aagcatgtca aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt
    12001 tgttttgatt tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc
    12061 gtggggacga aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac
    12121 tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta
    12181 gactacttag caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta
    12241 gcagatggat gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct
    12301 ggtatgtact caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa
    12361 tctcaattat tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct
    12421 agcgggattc attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag
    12481 ttttctttat catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag
    12541 ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga
    12601 gctgcacata tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct
    12661 atggctgcac aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa
    12721 aaatatggtg aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg
    12781 gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc
    12841 gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata
    12901 ggagaagaac gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc
    12961 tagccgatta tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc
    13021 agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt
    13081 cacctaaaga atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag
    13141 aaaaaaagat cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc
    13201 tgaaaaatta tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg
    13261 gtttgcatgg catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc
    13321 attatatcga ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg
    13381 aacaggagga cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct
    13441 atccagaagt gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt
    13501 gatcagtgta tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg
    13561 agtagaaatc atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac
    13621 gctttcgatc gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact
    13681 tcatcctaaa atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc
    13741 aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa
    13801 agaaacgatt atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg
    13861 tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga
    13921 tccatctgac tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc
    13981 tttcagacaa aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat
    14041 cgctcaatac ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta
    14101 tgaacgaaaa caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca
    14161 gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact
    14221 ttcttataat aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga
    14281 gccaacggta gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat
    14341 tttagaagcg tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat
    14401 cgttttgaat cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga
    14461 aatcatcatt gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa
    14521 catccgatta ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt
    14581 ctcagtctta ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga
    14641 gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagtgtttgc
    14701 ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac
    14761 agtaggaatc ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca
    14821 agcgcaagag aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga
    14881 ttcggttgaa tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat
    14941 caaagaccaa gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga
    15001 tgttcggcat tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc
    15061 aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt
    15121 gtattgtgcg ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag
    15181 tgagcgagat cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat
    15241 cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt
    15301 gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa
    15361 acaattaatg gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga
    15421 aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt
    15481 agcagctggg aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga
    15541 acaaatgttg aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt
    15601 tttggcagga gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat
    15661 ggtcatttcg caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg
    15721 aatgggtgct tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga
    15781 agaagtttta aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt
    15841 tttatatgcg ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg
    15901 tttcggtgat cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt
    15961 aatcgatgat ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa
    16021 tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct
    16081 accagatttt ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg
    16141 caaactagtt ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa
    16201 agaagcacag aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta
    16261 tcgtacagat attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg
    16321 cttccattta taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt
    16381 tcataaagag tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa
    16441 aaattcaatt aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta
    16501 tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt
    16561 tttttgacca tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc
    16621 tagcaaaaac atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc
    16681 caggtgtgat gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga
    16741 cccgtctgaa aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag
    16801 aaccagagga agtagctgaa gcgtttgatg gattggaacg gtcaggaaaa gttcgttatt
    16861 ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac
    16921 cattagtagc taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga
    16981 ttcatgtcaa tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt
    17041 cccgattgca tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag
    17101 gtgtatttat cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag
    17161 ctaaatatca aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca
    17221 atatgcaagt gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt
    17281 cagaaattaa tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt
    17341 taccttaaag attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact
    17401 tgaaagctgt tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat
    17461 cattgtacgg atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat
    17521 tcttttggag aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta
    17581 taaccaattt caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt
    17641 gctaattcga ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa
    17701 aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac
    17761 atatctggat cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga
    17821 tatttgttcc ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact
    17881 gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa
    17941 tgaaccattt cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc
    18001 aagacaaata aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt
    18061 tcgatgtact tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag
    18121 taaaagacag aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt
    18181 tgttctttaa gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt
    18241 aattcgctga ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc
    18301 ttttctaatt catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct
    18361 tcaagagaat caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact
    18421 tgttgattct ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag
    18481 tctgtactga cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga
    18541 ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg
    18601 ttcaagaaat gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt
    18661 tgctgcaaat ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta
    18721 atcggtttaa gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa
    18781 tcgtcgaatc cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt
    18841 gaaaaagtca acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt
    18901 aagtcgataa ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt
    18961 ggataataag aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg
    19021 tcttcgatga taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg
    19081 gatcatcagt gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg
    19141 atcatttccg taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact
    19201 gaaatctgaa tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg
    19261 gatgccaatc ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt
    19321 aatacgaata aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat
    19381 caatggctgc aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat
    19441 cttatagtca agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg
    19501 gctaatttct ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa
    19561 gccggaaagt gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga
    19621 aataatggca tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg
    19681 aacttcactt tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag
    19741 atcttccgcc attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag
    19801 ggttaaacga aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt
    19861 gatcggttct tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat
    19921 ggtactaaga actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt
    19981 attttttttg cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag
    20041 cagttgagtg acagaatcta cctgattgat gatctcttca atggtatcca taaaatcttt
    20101 tgccaagata ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag
    20161 cgatacatct aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat
    20221 atcattccta agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc
    20281 agaaatcaag tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc
    20341 tgctttttgg atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag
    20401 tatgctcagt agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa
    20461 aatctgtttt atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg
    20521 ggatgttagc tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta
    20581 atgtttttcc tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc
    20641 cgacatcagg tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca
    20701 tatcttcttg tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt
    20761 cttttatcgc agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga
    20821 tttcggataa atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa
    20881 taaaatcacc gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat
    20941 ctttttttag ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg
    21001 tcggtgtgga gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat
    21061 ttgtgaagtg attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc
    21121 caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga
    21181 cagggatccc tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag
    21241 gagtaaaaat gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc
    21301 gttcctgatt catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag
    21361 tgataattga ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac
    21421 caacgactag tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag
    21481 cgattacaac aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga
    21541 tttgttagta ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa
    21601 aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag
    21661 taaatcccat aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa
    21721 ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat
    21781 tttgaccgca agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac
    21841 ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa
    21901 caacatggtc tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga
    21961 taaagtagaa aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact
    22021 tgtgattgct tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc
    22081 agatatcaag gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta
    22141 cgcgactttt gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt
    22201 aggattgaaa gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga
    22261 caacaacgca ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa
    22321 cggtcagttg gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg
    22381 ggatggttct acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca
    22441 aacattggat gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt
    22501 aaaaggcgtg gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc
    22561 gacagttgct ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga
    22621 cacacgtatc ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga
    22681 ggttccggtc aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt
    22741 agcaaataca gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta
    22801 ttataaagaa tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa
    22861 gtcagagaga ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa
    22921 aaattttctg gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc
    22981 catgcattgt gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc
    23041 ttgtatccat atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt
    23101 aaagatttga aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta
    23161 agtccttatc tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc
    23221 gtgattgatt gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg
    23281 tctatcgacc cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa
    23341 atagctaaag cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca
    23401 ttgaatgaag aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt
    23461 gtaacctcca tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc
    23521 accattttac gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag
    23581 catattattc gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg
    23641 gatattggag aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac
    23701 gagcgaatca taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata
    23761 gctggactga tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat
    23821 ggaagcaaga tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt
    23881 ccttcagcca ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg
    23941 aatctgctga tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa
    24001 aaaggagtat tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg
    24061 cgtataaaaa caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa
    24121 aaagtcgttt tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca
    24181 acccgtggga tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca
    24241 gcaaatggca aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt
    24301 gatcgaatct atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca
    24361 gatcaggaag tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc
    24421 aaaaaatctt acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct
    24481 ttttggtcgc tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat
    24541 gacggatggt attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca
    24601 tatattagta ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc
    24661 ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca
    24721 aatgaacccg tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg
    24781 gcaaggtttt tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt
    24841 gctgatgttt cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc
    24901 agctgttttc caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct
    24961 tcacttcttg acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg
    25021 gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt
    25081 ggtaaaggcg gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta
    25141 tcaagggtat ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt
    25201 gacaaataaa acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc
    25261 tgaactatca gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt
    25321 catggcagct ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc
    25381 aggtacatcg atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc
    25441 cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa
    25501 caatggcatg tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat
    25561 cttgctttta gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa
    25621 cgccggttat tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa
    25681 aagcgaacat tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt
    25741 cgtataaatt ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc
    25801 gctaaaaaaa gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg
    25861 aggataatgt aatgaaagta tttgattacg aagatattca attaattcct aataaatgta
    25921 tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa
    25981 tgccagtagt gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag
    26041 cggaaaatgg ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta
    26101 tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg
    26161 aatattcctt tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg
    26221 acatcgcgca tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt
    26281 tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg
    26341 aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa
    26401 aaatcaagac aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga
    26461 aagcagctcg caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa
    26521 aatctgtccg ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg
    26581 aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg
    26641 cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac
    26701 acaaaggatc tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt
    26761 cttatgctgg aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa
    26821 attcgatttt taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa
    26881 aagttgtcct gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg
    26941 tttggctgga attattatcg cagatacaag cttttgaaca tcattattca gaataaggga
    27001 ctgtgataag atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct
    27061 cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga
    27121 aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga
    27181 aatcgaaaac ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca
    27241 actttttggt gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg
    27301 ggtactaacg agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt
    27361 cgctgttcca agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg
    27421 gtatgagcaa aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata
    27481 cgctagcgag atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga
    27541 agaaatctta gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa
    27601 aatcaaacgg tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt
    27661 gactggtggc ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga
    27721 ggggatagct gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg
    27781 aacgcgttta tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga
    27841 atcgatccat aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac
    27901 aggaaaagtg atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg
    27961 gcttccaatt attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt
    28021 ccttcctata cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt
    28081 tttaggaaaa acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact
    28141 agctcaagcg caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc
    28201 taatgtattt aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga
    28261 aaaaagccga ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat
    28321 tttaggtggt tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc
    28381 agaaaaagta gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat
    28441 aaatggaatc agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc
    28501 aaagatgaaa attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa
    28561 gaatataaca gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg
    28621 atataaagaa actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa
    28681 taaatgtttg caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta
    28741 agtttgatta gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat
    28801 tttattcact aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt
    28861 gtgcttttag cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat
    28921 agtgatacag ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag
    28981 agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag
    29041 atgaagtcaa tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta
    29101 acaaagattt agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg
    29161 tatggtctcg tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct
    29221 tttttgcacc aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata
    29281 aagaagatgt tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac
    29341 ctttatctca agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag
    29401 aattgaaagc tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg
    29461 ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg
    29521 gcggttcatc acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa
    29581 atgccgtatt ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa
    29641 ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg
    29701 atggaatgga tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca
    29761 gtggaaatgg accagtccta atcgaaacac tgacttatcg ttatggacca catacactat
    29821 ctggtgacga tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag
    29881 atccattgat ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag
    29941 aagaacaagt catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca
    30001 aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat
    30061 caatcaaaga acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa
    30121 aacaatgatc caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt
    30181 cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt
    30241 gcaggaaaag ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc
    30301 aggattgagt ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt
    30361 tggttttatt ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg
    30421 aatgagcggc actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca
    30481 tacaccagaa ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg
    30541 agtagtgatt ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag
    30601 taacgatccg gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt
    30661 gccggatgaa gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga
    30721 tgtatctatt atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct
    30781 tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt
    30841 agaaacgatc attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca
    30901 acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc
    30961 attagaagcc ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc
    31021 agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga
    31081 attttaagaa aagaagggac gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DACELF010000027        30463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.027, whole genome shotgun sequence.
ACCESSION   DACELF010000027 DACELF010000000
VERSION     DACELF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.027"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..552)
                     /locus_tag="IW687_001151"
     CDS             complement(<1..552)
                     /locus_tag="IW687_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0950426.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="IW687_001152"
     CDS             complement(678..2606)
                     /locus_tag="IW687_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0950427.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="IW687_001153"
     CDS             complement(2912..3628)
                     /locus_tag="IW687_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0950428.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IW687_001154"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IW687_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0950429.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IW687_001155"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IW687_001155"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0950430.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="IW687_001156"
     CDS             complement(7098..8558)
                     /locus_tag="IW687_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950431.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="IW687_001157"
     CDS             complement(8548..9843)
                     /locus_tag="IW687_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0950432.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="IW687_001158"
     CDS             complement(10175..10852)
                     /locus_tag="IW687_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0950433.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="IW687_001159"
     CDS             complement(10882..11973)
                     /locus_tag="IW687_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0950434.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="IW687_001160"
     CDS             complement(12074..12508)
                     /locus_tag="IW687_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0950435.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IW687_001161"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IW687_001161"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0950436.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16441)
                     /locus_tag="IW687_001162"
     CDS             complement(14546..16441)
                     /locus_tag="IW687_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0950437.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16499..18274)
                     /locus_tag="IW687_001163"
     CDS             complement(16499..18274)
                     /locus_tag="IW687_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0950438.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18324..19820)
                     /locus_tag="IW687_001164"
     CDS             complement(18324..19820)
                     /locus_tag="IW687_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0950439.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20014..21636)
                     /locus_tag="IW687_001165"
     CDS             complement(20014..21636)
                     /locus_tag="IW687_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0950440.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21964..23277)
                     /locus_tag="IW687_001166"
     CDS             complement(21964..23277)
                     /locus_tag="IW687_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0950441.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23522..23746)
                     /locus_tag="IW687_001167"
     CDS             complement(23522..23746)
                     /locus_tag="IW687_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950442.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23766..24134)
                     /locus_tag="IW687_001168"
     CDS             complement(23766..24134)
                     /locus_tag="IW687_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0950443.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24330..25040)
                     /locus_tag="IW687_001169"
     CDS             complement(24330..25040)
                     /locus_tag="IW687_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950444.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25058..25555)
                     /locus_tag="IW687_001170"
     CDS             complement(25058..25555)
                     /locus_tag="IW687_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0950445.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25592..26044)
                     /locus_tag="IW687_001171"
     CDS             complement(25592..26044)
                     /locus_tag="IW687_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0950446.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26179..26784)
                     /locus_tag="IW687_001172"
     CDS             complement(26179..26784)
                     /locus_tag="IW687_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0950447.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27234..27854)
                     /locus_tag="IW687_001173"
     CDS             complement(27234..27854)
                     /locus_tag="IW687_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0950448.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27856..28725)
                     /locus_tag="IW687_001174"
     CDS             complement(27856..28725)
                     /locus_tag="IW687_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0950449.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28748..29155)
                     /locus_tag="IW687_001175"
     CDS             complement(28748..29155)
                     /locus_tag="IW687_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0950450.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29174..30463)
                     /locus_tag="IW687_001176"
     CDS             complement(29174..30463)
                     /locus_tag="IW687_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0950451.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8630 a         6627 c         5021 g        10185 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    16201 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    16261 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    16321 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    16381 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    16441 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    16501 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    16561 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    16621 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    16681 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    16741 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    16801 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    16861 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    16921 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    16981 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    17041 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    17101 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    17161 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    17221 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    17281 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    17341 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    17401 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    17461 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    17521 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    17581 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    17641 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    17701 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    17761 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    17821 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    17881 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    17941 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    18001 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    18061 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    18121 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    18181 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    18241 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    18301 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    18361 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    18421 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    18481 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    18541 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    18601 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    18661 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    18721 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    18781 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    18841 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    18901 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    18961 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    19021 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    19081 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    19141 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    19201 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    19261 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    19321 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    19381 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    19441 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    19501 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    19561 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    19621 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    19681 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    19741 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    19801 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    19861 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    19921 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    19981 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    20041 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    20101 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    20161 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    20221 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    20281 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    20341 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    20401 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    20461 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    20521 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    20581 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    20641 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    20701 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    20761 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    20821 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    20881 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    20941 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    21001 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    21061 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    21121 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    21181 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    21241 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    21301 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    21361 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    21421 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    21481 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    21541 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    21601 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    21661 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    21721 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    21781 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    21841 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    21901 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    21961 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    22021 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    22081 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    22141 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    22201 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    22261 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    22321 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    22381 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    22441 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    22501 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    22561 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    22621 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    22681 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    22741 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    22801 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    22861 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    22921 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    22981 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    23041 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    23101 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    23161 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    23221 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    23281 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    23341 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    23401 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    23461 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    23521 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    23581 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    23641 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    23701 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    23761 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    23821 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    23881 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    23941 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    24001 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    24061 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    24121 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    24181 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    24241 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    24301 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    24361 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    24421 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    24481 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    24541 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    24601 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    24661 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    24721 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    24781 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    24841 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    24901 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    24961 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    25021 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    25081 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    25141 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    25201 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    25261 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    25321 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    25381 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    25441 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    25501 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    25561 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    25621 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    25681 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    25741 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    25801 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    25861 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    25921 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    25981 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    26041 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    26101 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    26161 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    26221 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    26281 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    26341 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    26401 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    26461 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    26521 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    26581 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    26641 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    26701 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    26761 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    26821 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    26881 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    26941 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    27001 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    27061 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    27121 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    27181 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    27241 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    27301 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    27361 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    27421 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    27481 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    27541 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    27601 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    27661 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    27721 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    27781 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    27841 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    27901 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    27961 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    28021 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    28081 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    28141 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    28201 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    28261 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    28321 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    28381 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    28441 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    28501 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    28561 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    28621 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    28681 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    28741 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    28801 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    28861 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    28921 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    28981 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    29041 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    29101 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    29161 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    29221 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    29281 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    29341 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    29401 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    29461 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    29521 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    29581 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    29641 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    29701 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    29761 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    29821 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    29881 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    29941 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    30001 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    30061 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    30121 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    30181 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    30241 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    30301 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    30361 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    30421 cctgagtcaa aatattttcg atgataaaat tgataacgcc cat
//
LOCUS       DACELF010000028        29977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.028, whole genome shotgun sequence.
ACCESSION   DACELF010000028 DACELF010000000
VERSION     DACELF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.028"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..399
                     /locus_tag="IW687_001177"
     CDS             22..399
                     /locus_tag="IW687_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950452.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1005..1619
                     /locus_tag="IW687_001178"
     CDS             1005..1619
                     /locus_tag="IW687_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0950453.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2392..4131
                     /gene="yfcC"
                     /locus_tag="IW687_001179"
     CDS             2392..4131
                     /gene="yfcC"
                     /locus_tag="IW687_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0950454.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4288..5472
                     /gene="iadA"
                     /locus_tag="IW687_001180"
     CDS             4288..5472
                     /gene="iadA"
                     /locus_tag="IW687_001180"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0950455.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5583..5786
                     /locus_tag="IW687_001181"
     CDS             5583..5786
                     /locus_tag="IW687_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0950456.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5887..6549)
                     /locus_tag="IW687_001182"
     CDS             complement(5887..6549)
                     /locus_tag="IW687_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950457.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6562..8064)
                     /locus_tag="IW687_001183"
     CDS             complement(6562..8064)
                     /locus_tag="IW687_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950458.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8612..10291
                     /locus_tag="IW687_001184"
     CDS             8612..10291
                     /locus_tag="IW687_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0950459.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10418..10897
                     /locus_tag="IW687_001185"
     CDS             10418..10897
                     /locus_tag="IW687_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0950460.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            11116..11796
                     /locus_tag="IW687_001186"
     CDS             11116..11796
                     /locus_tag="IW687_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0950461.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11804..13135
                     /locus_tag="IW687_001187"
     CDS             11804..13135
                     /locus_tag="IW687_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0950462.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            13150..14112
                     /locus_tag="IW687_001188"
     CDS             13150..14112
                     /locus_tag="IW687_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0950463.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            14223..14561
                     /locus_tag="IW687_001189"
     CDS             14223..14561
                     /locus_tag="IW687_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0950464.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14640..15347
                     /locus_tag="IW687_001190"
     CDS             14640..15347
                     /locus_tag="IW687_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0950465.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15450..16931)
                     /locus_tag="IW687_001191"
     CDS             complement(15450..16931)
                     /locus_tag="IW687_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0950466.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            17240..17776
                     /locus_tag="IW687_001192"
     CDS             17240..17776
                     /locus_tag="IW687_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0950467.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            17854..19224
                     /locus_tag="IW687_001193"
     CDS             17854..19224
                     /locus_tag="IW687_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0950468.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            19291..19626
                     /locus_tag="IW687_001194"
     CDS             19291..19626
                     /locus_tag="IW687_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950469.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(19707..20564)
                     /locus_tag="IW687_001195"
     CDS             complement(19707..20564)
                     /locus_tag="IW687_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0950470.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            20671..21186
                     /gene="tadA"
                     /locus_tag="IW687_001196"
     CDS             20671..21186
                     /gene="tadA"
                     /locus_tag="IW687_001196"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0950471.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            21209..21293
                     /gene="ffs"
                     /locus_tag="IW687_001197"
     ncRNA           21209..21293
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW687_001197"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21503..22888
                     /locus_tag="IW687_001198"
     CDS             21503..22888
                     /locus_tag="IW687_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0950472.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            22925..23602
                     /locus_tag="IW687_001199"
     CDS             22925..23602
                     /locus_tag="IW687_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0950473.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            23610..24374
                     /locus_tag="IW687_001200"
     CDS             23610..24374
                     /locus_tag="IW687_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0950474.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            24376..25035
                     /locus_tag="IW687_001201"
     CDS             24376..25035
                     /locus_tag="IW687_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0950475.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(25065..25574)
                     /locus_tag="IW687_001202"
     CDS             complement(25065..25574)
                     /locus_tag="IW687_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0950476.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(25686..26684)
                     /locus_tag="IW687_001203"
     CDS             complement(25686..26684)
                     /locus_tag="IW687_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0950477.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            26885..27469
                     /locus_tag="IW687_001204"
     CDS             26885..27469
                     /locus_tag="IW687_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0950478.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(27546..28721)
                     /locus_tag="IW687_001205"
     CDS             complement(27546..28721)
                     /locus_tag="IW687_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0950479.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            28803..29183
                     /locus_tag="IW687_001206"
     CDS             28803..29183
                     /locus_tag="IW687_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0950480.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            29257..29619
                     /locus_tag="IW687_001207"
     CDS             29257..29619
                     /locus_tag="IW687_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950481.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9758 a         5015 c         6211 g         8993 t
ORIGIN      
        1 aaaaaaaggt gtgtgttcac tatgggaaaa ttagtcgctg ttgcacagtc gctgcttgat
       61 atactcaagt ggctgatgga aggaagcaat tggatatttg tgttgccact attgcttatt
      121 agtctattta tcatgtttcg catggtcaga aaaaaagaaa cgtttctgat ttttatctgg
      181 tatgcagcat ccgttggggt tgctaccttg atggttgaat tggcgttagg tagttgggaa
      241 gcttgcgtat ttgctttatt ttttgctgta ctgtatctaa tcatttatat cccatatatg
      301 aaaagaaagt ctcggatagg cagaaaaggt agacatacaa ctactttttc tagtacagat
      361 tctctcgaac aaagtagaag aacgagaaga agacggtaat atttagtagg tatacgcaaa
      421 aaaagataaa ataatggata aactattgag atgagagatt tatttaattg gtggaaaaaa
      481 gcttttttca cttatataaa agaatctttt ttaaaggaat gcttgttgta tcgggataag
      541 ccattccttt ttttatgtta tttatatata tataaatcat accaatgttt ttttgtattt
      601 tcttgcttgc acttggataa gagattactt acaataaatt cagatagaaa cagctgaata
      661 aaaaaaggct ccctgaccct tagttggcgc taagaatcag ggaatgacca gtcacacaca
      721 tacaccctga ccgatcattt tgccatagtc aatgcagtgc atcgacctaa tttattgtac
      781 ctaatctagg gtgaaaattc tacccctatg tagatatttt ccatggtctg cacgttgttt
      841 tatttgctat gtcaaggtat tgggcgtatt cgttcaatgc cttttttgtg tttgaaacag
      901 caaagaccac atgtgttact gtctaactac tgttttttta tgtacttcct tagattaggc
      961 agctgtttct attcatgtca agtatagcta ggcgggagag ataaatgagt ttatgttatt
     1021 ttgactatta tcaccaattg gaagagaaat taaggataga agagccgctg atgcttcaag
     1081 ctggcacttg gatcgcgaaa caaattaatc aaggtggtag attgcagata tatgctagcc
     1141 gcacactaag tggtgtagca tttgaatttt gggatcaaac cccggatctt gtaccaagta
     1201 tattgattga aaatccagcc ggaggtgtct atgaaacgct agaaggtgca ggacaggcaa
     1261 ttattgatca gctttctgtc aaatcagaag acatttttct tcttctttcg aatgaaggaa
     1321 gaaacccggc aatcatcgaa ctagctcagt ggatcaaaga aaatggtcat ccattaatca
     1381 ttgttactgg atttgatttg tcacgaagta tcaaatcgca acatagcagt ggtttgcgat
     1441 tatttgaatt tgctgatttg gttttggaca accacgccaa tttacatgat gcagtcttga
     1501 ctttgccaaa tctagatcca gcgatctgcg gaacagcctc tcttgcaacg atctttttgc
     1561 tgcagcaagt tttgtatttt acagcaaaac agctagtcca ggacacaagt ggaaaatagt
     1621 tatccacatg ttgacaaagt tccttaaaaa aataatgttc cacataaaaa tggagcgttt
     1681 gaaaaaaaga aaacgattat taatgaggat taacaacgac tacttttttt gttttagtta
     1741 ttttaagatg gatcatcttt gattttaagc ttgttttgtt taaagtttgt tatttttata
     1801 agttgtttca cagttatgag aaatgagtta taagaatatg tagtgtcaga aaaataaaaa
     1861 gtagtggaaa aagagacaag aaagaatcca tatggattct ttcttgtctc ttaatacacg
     1921 ttgatgaaag agtcttcccc aaaactcttt acaaagtaat tatagtacat ttgttatcat
     1981 tattgcaatg aagtttacct gatataacaa aattgtccaa caggatacct ttttctaata
     2041 gaaataaaat acacaaaaat tgaaggatag ataatggata tttataagtg tttattattt
     2101 tttttgcaat gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt
     2161 tctctaatct aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat
     2221 ataaatggaa agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag
     2281 tgattttcta ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt
     2341 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
     2401 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
     2461 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
     2521 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
     2581 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
     2641 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
     2701 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
     2761 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
     2821 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
     2881 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
     2941 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
     3001 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
     3061 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
     3121 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
     3181 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
     3241 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
     3301 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     3361 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     3421 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     3481 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     3541 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     3601 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     3661 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     3721 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     3781 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     3841 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     3901 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     3961 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     4021 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     4081 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     4141 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     4201 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     4261 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     4321 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     4381 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     4441 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     4501 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     4561 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     4621 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     4681 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     4741 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     4801 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc
     4861 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     4921 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     4981 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     5041 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     5101 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     5161 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     5221 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     5281 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     5341 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     5401 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     5461 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     5521 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     5581 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     5641 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     5701 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     5761 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     5821 aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     5881 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     5941 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     6001 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     6061 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     6121 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     6181 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     6241 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     6301 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     6361 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     6421 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     6481 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     6541 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     6601 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     6661 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     6721 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     6781 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     6841 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     6901 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     6961 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     7021 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     7081 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     7141 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     7201 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     7261 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     7321 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     7381 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     7441 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     7501 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     7561 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     7621 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     7681 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     7741 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     7801 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     7861 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     7921 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     7981 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     8041 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     8101 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     8161 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     8221 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     8281 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     8341 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     8401 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     8461 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     8521 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     8581 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     8641 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     8701 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     8761 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     8821 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     8881 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     8941 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     9001 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     9061 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     9121 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     9181 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     9241 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     9301 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     9361 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     9421 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     9481 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     9541 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     9601 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     9661 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     9721 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     9781 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     9841 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     9901 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     9961 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
    10021 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
    10081 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
    10141 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
    10201 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
    10261 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
    10321 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
    10381 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
    10441 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
    10501 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
    10561 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
    10621 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
    10681 ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa
    10741 actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt
    10801 aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa
    10861 gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca
    10921 gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc
    10981 tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta
    11041 tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa
    11101 cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg
    11161 gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta
    11221 actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata
    11281 ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt
    11341 gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat
    11401 gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta
    11461 gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa
    11521 acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa
    11581 ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat
    11641 gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact
    11701 ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga
    11761 aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga
    11821 tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat
    11881 cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa
    11941 tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa
    12001 aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt
    12061 aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa
    12121 acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga
    12181 agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac
    12241 gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta
    12301 tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa
    12361 agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca
    12421 gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc
    12481 agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa
    12541 atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac
    12601 aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt
    12661 cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat
    12721 gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa
    12781 tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt
    12841 cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga
    12901 aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat
    12961 tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg
    13021 tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga
    13081 aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc
    13141 aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc
    13201 attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc
    13261 gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa
    13321 gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta
    13381 gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca
    13441 gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg
    13501 gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct
    13561 gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat
    13621 acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg
    13681 agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa
    13741 gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga
    13801 agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct
    13861 tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag
    13921 cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg
    13981 gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta
    14041 gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga
    14101 aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc
    14161 ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga
    14221 agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct
    14281 tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag
    14341 aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga
    14401 cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag
    14461 tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg
    14521 gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt
    14581 cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa
    14641 tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat
    14701 tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag
    14761 tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac
    14821 agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag
    14881 gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag
    14941 gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga
    15001 agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga
    15061 ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt
    15121 tttctttagc attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc
    15181 gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa
    15241 gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc
    15301 agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg
    15361 tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt
    15421 tggaataaga ggatgtgaca agaattttgt cacatcctct ttgttttgtt ctcataacct
    15481 tttttcatat acagttcatg aaccgctttt attaacttgc caaataacac tgtattgtgc
    15541 gttgtatcca gatagaaagt ctccgtttca tctgaaattt tctcatgctg atctgtaatg
    15601 atcaaatcag catgttctga atatgcggta attttaacag catctttgcg aaataactgc
    15661 tgcaaatggc tttgaagatg ataatttcct gtaaactgtt tggttatctg caaatggatg
    15721 agtaactgct ctttcgtttc tgtttgtagt aagtgttgga tcaattgact aaggtattga
    15781 ctgatttggt ttctttcccc attttttgaa aaaattgtat cgattttttc tcttaatttc
    15841 ttttcctgta taaaagaact agaatgattg gacgtggctt ctggaaagca aagtgtagaa
    15901 aaactactgc tggattcacc caacagttcg aaaaaaacaa ctgccaacac aagaaaataa
    15961 aaataaattg gtattttatt ttctaaatgg ttgagaggaa actgttgagt taagtaagtc
    16021 atcaattgac cgacttgtcg acaaataagg tttttgcttg tggaaaataa aaaaccaatc
    16081 tcctgttttt gtcgaaaaga gatttgattt ggaaaaaaac aatagaaaaa ataattaaag
    16141 taggctctct ctgtttctgt ctcttcacaa gggcgactca ctgaaaaaga agtaaagtat
    16201 tctgaataat catgtgtatg ctgcaataag tccatcaatt cagtgatttc agaagaagtt
    16261 tttggaaaat atgcctgttg tttgtttcgt aatatgaata aagttaacag caagtagata
    16321 tttttttctt gttttgaaat tttttctatt tgctcatctg tactgttcat ttttagtgca
    16381 tttaattcaa gtgaggaaaa tggccaatcg ttacctccaa atgtatctga aagaaaaaag
    16441 taaagataga gacggataga taactcattt ccttcaagag tacagcaatt ttttacagat
    16501 aggatccgta aatggaattt ttttaaggaa tgattgagct gttttgtcag tttttcgaga
    16561 taggagggcg atatataaag ttgttcaagt aaatattttt ttggtacagc agtttgtgga
    16621 taattgatta gtaacatcaa atagttaaaa cgattggatt cttttaggta gcataatttt
    16681 aacttagaaa aaacagctaa ctttttagtt tgatcggttt ctttggtttg gatctctcct
    16741 ttatgattag aataaataaa ttcatcaatc aataaaaatt gcttgagtga tacatttatt
    16801 tttcggatca gttctttgat tgtccgtttt ggaatatgta agtcattgga taattcttgt
    16861 aaagaacaat tatttttttg aatgaccatt tgtagaacaa gtaactcttt ttttcttgct
    16921 tcaattagca taaatacctc ctttttattc tgtttttgta tatagttaga caaaaactgt
    16981 gagtggaaag tcatttcagt ctgtgttatt ctcacagagt gtcgccttct tttttatata
    17041 acgttattta atttttttgt taccttgttt attctattgt ttatttccta gggtattttg
    17101 tcaagatatt cttgttttta aggcggaaaa attgattttt aatggatata actaaatatg
    17161 ttttgataat tatcaataat atttttagaa ttatttatgt tatttattta tgggtagatg
    17221 aaaagagacg gaggtaagaa tgatggggag agtaacaaat tatagtaaag aatatttgct
    17281 gttcaaaagt atggtatacg tggaagaata tggaatgaag tcaattactg caagagagtt
    17341 agcagagttc tgcggttgtt ctacataccc gatttataca catttcaaat caatcagcgg
    17401 gttaaaagag aagatattag aagaaatcac tgtttatttt gaaaactact tagctgaatg
    17461 cagttccgat gatgttttaa gcattgtcta cttattgaaa gatttttttt ctatccatga
    17521 aagtattcgt gaaatcattg caaaatctga tttagacatg gattcgctat ttaagcggcc
    17581 atttttctca tatgtcaaag gtcacacgaa gatcaaagaa gaaaagtcgc ttgaattggt
    17641 atggcttaat gcattgggaa gtttgcgctc agatgattct tctcaagatt ttcttgaaat
    17701 gatgaacgct gtttgggata aaaacgaggt gacggatgaa tacttggaaa atatcttaaa
    17761 ggaacagtca ccttaggcag caacatgaaa aagtagatca gccaccttta tttttcgtaa
    17821 aaaagattgt aaaatgtcta acgaaacatt ggaatgaagg aggaagcgac aacgttacca
    17881 cagcaattta tcaaaaaata tcgtcttctt ttaggcgaag aagcatccga ttttttttct
    17941 gcgcttgaac aaggaagcgt gaaaaaagga tttcggtgga atccactaaa accggcagga
    18001 ttggacatgg tccaaactta ccatagcgaa gaattacaac ccgctcccta cagcaatgag
    18061 ggatttcttg gcactgtcaa tggaaagtct ttcctccatc aagctgggta tgaatatagc
    18121 caagaaccaa gtgcaatgat cgttggaaca gctgctgcag caaaacccgg tgaaaaagtt
    18181 ttggatttgt gtgcggctcc cggtggtaaa tcaacccaat tagctgctca aatgaagggg
    18241 aaagggttat tagtgacgaa tgagatcttt ccgaaacgtg cgaagatttt atcagaaaat
    18301 atagaacgat ggggagtttc gaacgcaata gtgacaaatc atgcacccgc agaacttgtc
    18361 cctcattttt cgggcttttt tgatcggatc gtggttgatg ctccttgttc aggagaagga
    18421 atgttccgaa aagatccaaa tgcaataaaa gagtggacag aagaatctcc gctatactgt
    18481 caaaaacgtc agcaagagat tttgtcttca gcaataaaaa tgctaaaaaa taaaggacaa
    18541 ctgatttatt cgacttgtac gtttgcacca gaagaaaatg aagagatcat tagctggctt
    18601 gtagaaaatt atccagtaac gattgaggag attcctttga ctcaatccgt tagttcagga
    18661 cgttctgaat ggggaagcgt agctggtttg gaaaaaacaa tacgtatatg gcctcataaa
    18721 gatcagggtg aaggacattt tgttgcaaag ctgacttttc atggacaaaa tcagatgcat
    18781 aaagaaaaga agacacgaaa aaaaagtaaa gtgcaaatga caaaagagca ggaaaaactc
    18841 tggacggagt tttcaaatga ttttcattat gaagctactg gaagactttt agtgttcaac
    18901 gaccatcttt gggaagttcc tgagttagcc ccttctctcg atggtttaaa agtcgtacgt
    18961 acaggtttgc atttaggaga tttcaaaaag aatcgatttg aacctagcta tgcattagcg
    19021 cttgctacaa aaaagataga gaatatacct tgcttgccga tcactcaaaa ggaatggcaa
    19081 tcttacacag caggagaaac atttcaacgt gatgggaatc aaggatgggt tttgctagta
    19141 ttagataaga tacctgtcgg atttgggaaa caagtaaaag gaacagtaaa gaactttttc
    19201 cctaaaggat tgagatttca ctgagtggat tagttcgttt atttttttgt ctatgctatg
    19261 atggagatag ataaaaagga gctgatactt atgaaaacat caacaaaagt aacaatcgga
    19321 ttaagcgtag cagccgctgc cagtgtagcg acagcagtcg ttgtatcggg aaaagtaatg
    19381 gaaaagatcc atcatatgac tactcggacg aaagtaaaaa aattcgtcta tgacaaattt
    19441 gatggaaatg aaaaattact agaaatcgta gatggtcttt cagatagtga cttggattct
    19501 ttgatgggaa tgttagcaaa aatcaaatca ggaaaaaaga aaatctctgt ttatggtgat
    19561 tcaatcaaag attcaaccga agatgtaaaa gaccggctaa tgcattttgt tgaaaaaatg
    19621 atgtaagaga aactagcttt tgaacactat tcattcgaaa taaggggttg tgacaaaatt
    19681 attgtcacaa cccctttaat ctatctttaa ttatgttcat gaagcaataa aagatagcaa
    19741 aacagtaaat cgttggcttt ttttagattg aaaccagact gttcttgaaa tttttcgatt
    19801 cgatatttta gagtattacg atgaagaaac agtgtttttg cagcagagct gacattgcct
    19861 aattcattcc ataattcttg aatgatcact tccatttcat tttgtttgat cagttctatg
    19921 taacttttga tcaacttact atcttttaca gtatcttttg tatagtaatg taaagccaca
    19981 tcttgcatag aaaaagtacg gctgtgagta taaagattgt tttgctctgt taaaaaaatt
    20041 tttctttctt cagcaaacaa agggacgagg tcggtgccag gtgaatggaa agaaccaacg
    20101 aatacagacg ttgtcgtatc aaaatcagca tctaaagata gaaaaatacc gttgatttca
    20161 gtagcatcaa gataatcacg gtttatttgt tcgactaaga gcgcatctgt gtctgtataa
    20221 aaaaagaaat cagcagaata gcgaaataat tccaaaatag tatcttgcca ttcttttttt
    20281 aagaattctc cttttgattc gacgtgaagc tggatcaaac gaaagctttt ttcttgaaca
    20341 caagaagcat ctttaaaaag atagtgatac catgggtgtt ttttttgatc gttgattaca
    20401 gggaataacg ctttcaaaag gctggtttct tgttgagata atgaatcttg tttgatccag
    20461 ataaaagcat catcaattgc aaaagaaagg atatccgctt ctgatgctgg agagtttttt
    20521 ttctgtgctt gtgggtaaag agcaagtaat ttatccattt tcatcttgtt cacctcttgt
    20581 atttatttta ccatagaaaa aaaagttagt tacagccttt ttatcatatg ctaaagtaaa
    20641 acaagaaaaa acaaaggcag ggaagggtaa ttggtaaata caaatcttga tgcagtagaa
    20701 aaagaaaaat ggatgcgcct tgcattggca gaagcaaaga aagcagaaat gcttcatgaa
    20761 gttccgattg gagcagtggt tgtattggat ggaaaagtga ttggaagagg atataacctt
    20821 agagaaacca cgcaagatgc tactacacat gcagaaatgc ttgcaatcaa ggaagcctgt
    20881 gaaaaagtgg gaagctggcg cttggaagat gctgcacttt ttgttacctt agagccttgt
    20941 cctatgtgca gcggcgcgat gattttgtct cgtgtagcag aagtttattt cggcgcgtat
    21001 gatccaaaag gcggaacagc cggtaccttg atgaatcttt tggaggacga gcggtttaac
    21061 caccaagcat atgtagaagg tggcatttta gaagacgaat gtgggatgat tttgactgac
    21121 tttttccgaa agctccgcca gcgaaaaaag aaattaaaaa aagactggtc aacgaatgaa
    21181 aaataaggta tactattctt tgccgaaagg ctaggacaat ggtgggcttg tgaagcgtgt
    21241 caggtctgga aggaagcagc actaagcagg ttccgccatg tgtctgatta taatgaaacg
    21301 acaacagact ttcgggtctg ttttttttat ttttatagta ctgtaaaaat gaatgttcct
    21361 gattttttag ccagactgat ttatcgaata ttgtacttat acaatttgct tcaaggcagg
    21421 aaaaactctg aaatagagct tgtttcaatg gaagaaaaag ctgcctatgg tatgataaag
    21481 ataaagagag gcgagagggt acatggaaaa aatagtgatt ttacatacga atgatctgca
    21541 ttctcattta gaaaattggc caaggatcag aaggtttttg gatcaacgta aacgagaaaa
    21601 cgaaaagaaa gaaaaaacga caacgattgc agtagatcta ggtgattttg tggatcgttg
    21661 gcatccattg tcagaagcaa cgaatggaca ggaaaatatc ttgctgatga atgaagttgg
    21721 ttatgatgca gcgacgatag gaaacaatga aggcgtagga aattccaaag aagagttgaa
    21781 tcatttatac gatcatgctg agtttgatat catcctggat aacttattcg ataaaaatac
    21841 gctccagcca ccggcatgga cgaaaccata taaaatcatt acaacgaaac accagacgaa
    21901 aatcggacta attgcattta ctgctccatt tccgcttact tataatccga atggctggga
    21961 catacggaat ccttatgata ttttgccaga attagtcgaa gatttacgtt cacaagtgga
    22021 tgttttagtt ttgatgagtc atctcgggat tcaggatgat ttccaaattg caaaagaaat
    22081 accagcaatt gatgttattt taggatcgca cacccatcat ttattcagag aaggtcacgt
    22141 aatcaatgat gtccaaatag cagccgctgg aaagtacggg cagtatgtcg gtgaagtcca
    22201 tttaaccatc gatgaagaga aaaaaatcct taagcattca gccaaagcca tccctaccga
    22261 aacaatgaca gcttttacag aagatgaaca agaaataaat agttatctag aacgtgggca
    22321 tgaattatta aaagaaaaag aagtagcaga tttgccattt tctctgagtc tagatatctt
    22381 caatgagcat tcgtttatcc aagtagcctt tgaggcggtg aaagaacgag gaaagacgga
    22441 agcagcaata ttaaacagtg gtctgttttt aactgatatt ccaaaagggc gggtcaatca
    22501 ggatcagctt cacactgcgt tgccgcatcc gatgcacttg ttaaatgtca cattgggtgg
    22561 aaatgatctg atccgcttag ttttagaaat agaaaaaaat cgtaactttt tacgtaacta
    22621 tcctatgaag ggaatgggat ttcgcggtaa gatttttggg cagatggtct ataatggaat
    22681 cacatatgat tctattaacc atcaagtctt ttggaagaac aagccaatcg atctgaataa
    22741 aaattatact tttactaccg tagatcattt gatgtttgtt ccattttttc caacgattga
    22801 gattgctgga aaaaatgaat ttttgttccc tgaatttatc cgaagtgtgg taggagatta
    22861 cttgaaagct cactacccaa tcaaataaaa acaaggtata ataaaaaaga taggtggtga
    22921 acgcatgtct gcaaaacaaa atgaacagca gaacgttgag cataaaacag aaattgctaa
    22981 gaaaaaatca tcagctgaac aggaaacaac agtcaccgaa gaactgacag ctgctttgga
    23041 agaaatcgtt gaggagccta aagcagaaaa agtcaaaggc gagctagtga cactgttaga
    23101 tgacagtaat tttttgatag gtgaaagaca ttatcggctt gtgtccgatt atcgtgaagg
    23161 atttaatgca gaaaagcttg gagaacgtta cagtgatgta ctagcccgat atgattatat
    23221 cgtaggagat tggggttatg aacaactacg cctaaaagga tttttccaag cggataatcg
    23281 acgggcgcat ccagatcagc gaatcgattc cttggaagac tatctttacg aatattgcaa
    23341 ttttggttgt gcgtattttg tcatcgagag aatcggcggt aaaaaagaaa agacaactca
    23401 gcgccgaaaa aagaaaaaaa accataatcg tgcgtatatc gatgagaaaa aaggaccagt
    23461 cgcatctaat cgcaaaaaac cggttattaa aaatcgaaaa acagagtcag caaaaacctc
    23521 tgaaaaagta aaaaaacaag aacaacctaa acaagtagaa aaaacaaaaa cagcatttac
    23581 gatcagaaaa agggaagagt agtggatgaa tggactataa aggatattta attgatttag
    23641 acggaacgat ttatcgtggg acacaaccca taccggccgg taaacgattt gtggaagaat
    23701 tgcaaaaaag aaaattacct ttcttgtttg ttaccaataa cacgactaag tcaccagaaa
    23761 cagtagctaa tcgtctagca gatgagtttg acattcatgt agctccagag acagtctaca
    23821 cagctacttt ggcaacaatc gattttatga aagcagatgg aaaagggaaa aaagtctatg
    23881 ttatcggaga agcaggattg attgacttga ttttagctgc cggatttaca tgggaagaag
    23941 aggctcctga ctatgtagtg gtaggcttag ataattatct gacctatgaa aaagtagtta
    24001 aagcaacact tgctattcaa aaaggtgcta ctttcattgg aacgaaccca gataagaata
    24061 tacctactga acgcggcctt cttcctggag cagggtcagt catctctttt gtagagacag
    24121 caacccaaac gccgccgatc tacatcggaa aaccagaagc catcattatg gataaagccg
    24181 ttgaagtatt agggctgcaa aaagaagaag taatcatggt tggagataac tatgaaacag
    24241 atatccaagc tgggatacgc aataatatag atactttgct cgttttatca ggttttacaa
    24301 aagaagaaga tgtacctggg ttgcctatcc cagctaccta tgtgaaacaa tcactggatg
    24361 aatggagctt ctaatatgaa aaattcgaga tggcagtgga tggaatatgc tggattgttc
    24421 agcctatttc tgacactgat cagtctggca gttggtgtga cgatcaactt tcgaccacta
    24481 tacgtatttg atatcggaca tttgcaaatt ttggattata cttctttaga tcaagagacg
    24541 ttactgaaaa attttgatca cctaatgaat tatctcaata atcccttcaa aaccatcctc
    24601 agcttgccag atttcccagt ttctgctagc ggagcccatc acttttatga agtgaaaatt
    24661 ttgtttttag tggattatgc tgtgtttttc atcactttga tcccgagtat tttgtttatc
    24721 aaatatttgc aaaagaatga tcggctttgg cggttgattc gtccgtttca aatcggcatg
    24781 ttactgccag ttgttttcgg ttttttcatg atgataggat ttgatcgctt ttttatcctt
    24841 tttcatgaaa cgtttttcaa caacgatgac tggttattcg atcccgttac tgatccaatc
    24901 atcaatgtac ttccagaaga atttttcatg catagcttta tcttattttt tgtattatta
    24961 gaattatttt ttgctgtttt tcttttctta ggaaaaaata gcttgaaaca aacaaaaaag
    25021 aaagaactcg tctaaataga cgttttcttt ctttttttgt tgaattattg agtaaggcga
    25081 cttctttttt ctggttcttt gtgtgctaat cgactggcag cagcagcggc gatcgctccg
    25141 acgatatcat ctaagaaggt atggacatga acgccatcgt ggctgtttag ttcttttaaa
    25201 ataccaggtt tcaccttgtc gatataacca taattcgtaa agccgatcgt accgtaaaca
    25261 ttcacgattg acaaggctag gatctcatcg ataccgtaaa ggccttcgtc atcttcgata
    25321 atatgttgca acggactcat caattttttc ttttctgcta atatatctaa ttggattcca
    25381 gtgataatgg cgttatgtac ttcacgcttt gttagcacgc tgttgacact ttcagtacaa
    25441 tccgccaaag tcaaatcaga aatatagtct ttttgtaaaa acatgacaag ttgtgcgata
    25501 tcttctacag ttacgccacg ttccttcaat agttctcttg ccttttcttc taaagtctca
    25561 gttttgacga ccatattatt gctccccctt tattttttct ttctctattt catgttaacg
    25621 gaacatgaaa aaaatgcaag aaactgcttt cgggtttaac ttgaaatgca aagagatata
    25681 aaaaatcaac taggacgaag atttttatcg tataagcttg tgctgtgacc aggctgagtt
    25741 cgttcctttg gattaatata gtgtaaagcg ttgttcactg cagtaggtgc ttcaccgagc
    25801 ccagtagcaa tcagtttcac ttttccttca taggtacaga tgtcaccagc tgcataaacg
    25861 cctggaatag aagtagacat atctgatttt acagtaatgg catttctggt actatcaagt
    25921 ccccaagaag aaagatgttc taaattagaa gagaatccat aattgacgat cagagaatca
    25981 atcatcaaat caatcgtctc gtcacttttc acttttttta accggatatc tgtcagttcc
    26041 ccattattgc ctgataatcc atcgatgaga tagggagtaa gcaagttgac agatgaagat
    26101 ttcaagcgtg atacactatg ttcgtgcgca cgaaattccg ggcgacgatg aatcaagtaa
    26161 acttcgcttg caataggctc taacatcaac gtccaatcga tagcagaatc accacctcca
    26221 gcaatagcga ctttttttcc agcatacttc atcaaatcat tcacgaaata atccaatccg
    26281 tggttttcaa atgattcagc attatctaaa ttcaattttc ttggctggaa tgaaccattt
    26341 cctaaagcta aaatgactgc tttggaataa tggatgcctt tatttgtagt aatctcgata
    26401 acttcgtctt cacgagtaag cgagagaact tcttctttta aatggaatgt atggttaaaa
    26461 gtagttaatt gtttttctaa ttgatcgatt aattcacttg cttttattgc tggataccca
    26521 ggaatatcgt aaatatattt ttcgggatac aaagttgcta attgaccacc aagttgaggt
    26581 agactgtcaa tgatttttgt tttagcttgg cgcattcctg cgtaaaaagc ggcaaacatc
    26641 cctacaggac ctgcaccgat gattgtaatg tcatagattt ccataaaaat tcctcccttt
    26701 tcacactata gcagaattta attgagaatg cttctcaaaa atgtttgaat tcgaaatcga
    26761 aacggtaagt tatagaatgt atctagtgat gaaaaataaa agaaaacaaa tctagtattt
    26821 ttacgagagg ttaggtatta tgttattatt attatgaagt atgcaaatat taggaggaaa
    26881 caatatggca ttcccacaac tagatttaaa aagtgaaaaa ggaccaaaag ctgttatcaa
    26941 aacaaaccgt ggtgacatta ctgttcaatt attccctgaa ctagcaccga agacggttca
    27001 aaatttcatt gaactttcca aaaaaggtta ttatgatggc gtgatttttc accgcgttat
    27061 tcctgatttc atgatccaag gcggcgatcc aactggaaca ggaatgggtg gcgaaagtat
    27121 ttatggcgaa agttttgaag atgaattcag tcgtgaactg ttcaaccttc gcggagcttt
    27181 atctatggca aattcaggtc caaacacaaa cggaagccaa ttctttattg taaataacac
    27241 aaatgtgcca gctaatatgc ttggacaact agaaggtgct ggattcccat cagaaatcat
    27301 tgaagcgtat aaaggtggcg gaactccttg gctagatttc cgacatacag tctttggaca
    27361 tgtactagaa gggatggaca cagtagatga aatcgcaaat gtacaacgtg gtccacaaga
    27421 ccgacctgta catgatgtag tgattgaaac aattgaaatt tcagaatagt ttggtatggt
    27481 acggagtaat tatgaaaaag gacgaaacct agtaaggtct cgtcctttta ttttgaataa
    27541 atgatttaat cgattgtctc taaagcatac ttgattcgat taagtccttc cagtaaagtt
    27601 tcttctggac aagcaatatt caagcgcata tgcccatgac cggaaggacc aaacgtgatg
    27661 ccaggattta atacgacttt tcctttccga acaagagtgt tctccaattc ttggtcgtct
    27721 gaaatatagg cggtaaaatc taaccacatt aagtaagttc cttcagggtt cattattctc
    27781 actttcggaa gatactgttc aaaaaactga ttaactgttt gaacattttt ttctagataa
    27841 ggaatcagtt ggcttaacca ttctccaccc gtctcataag ccgcttgggt tccaatcagt
    27901 ccaaaagtgt tgatttcgtg ctgttggttc atcactaaat gtttttcaaa atcagctttc
    27961 atctctggat ttttgataaa taccattgaa tttttgattg cagctagatt gaaagtcttg
    28021 gttgcggaag taaaagcaac tagtagatca tctaaattag gatcaatcgt tagcatggaa
    28081 gtaaatgtat gatcgaataa agttaaatct tgatggatct cgtcactaaa cagaatcact
    28141 tgatgtttta aacacaattc gcttaactgt ttcaattctt ctttgctcca aacacgccct
    28201 ccaggattgt gaggattgca taaaatcatt gcttttacat tgttttcttc gatttttttc
    28261 tccatgtcag acaagtccat gatgaaatga ttattttttt ctagcaaaga gcttcgaacg
    28321 atgtgacgtt gatttgtttc tataatagag gaaaatggag gataaactgg gtcatgaatc
    28381 aaaatagaat cgcctggttt tgtaaaagtt tgtactgctg cagctaaact ggttaacaca
    28441 ccacttgtaa aaacgatatt ttctttcgtc aactccacag catggtgttg gcgttcccaa
    28501 tgaatgatag cctgatatag tttatcagac agagtggcat aaccaaagat tccatgtttg
    28561 atgtattcac taaacgcttt agctacccca ccgggagaca agaagtccat atctgctacc
    28621 cacaaaggaa gtaagtcatc catttgatat gtctcagcaa ttgcgtccca ttttacactt
    28681 tctgtatctt ttcttgaaat ctgtttatca aattcgatca tcttaagacc tccagtcatt
    28741 ttcgtcgctt ctttttttga gctatttaca gtataatgat aaatgcgaag agagggaagt
    28801 agatgactta taaaattgga caaataattg aaggaaaagt gacagggata cagccttatg
    28861 gcgcattcgt ttctcttgat aacgagacgc aaggacttat tcatgtttca gaagtacaat
    28921 ccggttatac gaaaaatatc cattcattgt taaaagtcgg acagccagta acagttcaga
    28981 tcatcgacat tgatgaatat tccaaaaaaa tcagtttatc tttaagaacg ttagaaaaaa
    29041 caaatccagc tgttccatat cgcagaaaaa ggtatttcac gaataaaaat cgtaagatcg
    29101 gatttaccac tattgcggag cagttaccta tttggataga agaagcatta tctgaactag
    29161 aacggaaaga aaataagaaa taaaaagtgt attttttctg aaaatgtgct agaattttta
    29221 tgtaagccat agaacaagtt gatcgaggtg gaaattttgg caagcaaaag agttgctggt
    29281 acaattatgt tgcatttgga ggatggtact aaaaaatttt tggtccattc aattgatgac
    29341 aagttggaat ttgcgttagc ggaactttct gaaggaaaga ctggtttagc gaatatactt
    29401 aatttcttaa aagaaatagt tcatatcgat gttagcaaga ttagtttgat ggaattgaca
    29461 aatacacata ttaaagagga aaatgttccg ttgtttgtct ttgaaactga acaaaagaat
    29521 caaagagaag aattaggcga aggatatatt tgggaagaac caggtcaaat gcgctctgtt
    29581 cttggaactt acgaagtaga gggagtgcct ttcttctaaa atacagatta agcgaacaaa
    29641 gaagtttgaa tttttttcaa acttcttttt ttattgtttg gtccaaaaaa tacaatgaaa
    29701 tgttctgcta aagatagttt tttagtgcat tttattttca aaatatgttg aaaacggttg
    29761 tataacgaat aataataatt gatcgaaaaa tcatttcaca aagtaatgct taatttatag
    29821 ttgaccaagg aagaaataca tgataaattt aacagcgttg attctgaagt ggatgttata
    29881 tcacatttct aaaaaattta aaaaatagtt attgacatgt tactaacgac atgataagat
    29941 aactgagtcg ttaaaaaaga accacttgcg aagcttg
//
LOCUS       DACELF010000029        29100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.029, whole genome shotgun sequence.
ACCESSION   DACELF010000029 DACELF010000000
VERSION     DACELF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.029"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1162)
                     /locus_tag="IW687_001208"
     CDS             complement(98..1162)
                     /locus_tag="IW687_001208"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0950482.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1159..2244)
                     /locus_tag="IW687_001209"
     CDS             complement(1159..2244)
                     /locus_tag="IW687_001209"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0950483.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2257..3234)
                     /gene="mvaD"
                     /locus_tag="IW687_001210"
     CDS             complement(2257..3234)
                     /gene="mvaD"
                     /locus_tag="IW687_001210"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0950484.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3227..4171)
                     /gene="mvk"
                     /locus_tag="IW687_001211"
     CDS             complement(3227..4171)
                     /gene="mvk"
                     /locus_tag="IW687_001211"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0950485.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4493..5146
                     /locus_tag="IW687_001212"
     CDS             4493..5146
                     /locus_tag="IW687_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0950486.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5231..6136
                     /locus_tag="IW687_001213"
     CDS             5231..6136
                     /locus_tag="IW687_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0950487.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6138..6770)
                     /locus_tag="IW687_001214"
     CDS             complement(6138..6770)
                     /locus_tag="IW687_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0950488.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7090..7614
                     /gene="infC"
                     /locus_tag="IW687_001215"
     CDS             7090..7614
                     /gene="infC"
                     /locus_tag="IW687_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0950489.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7686..7886
                     /gene="rpmI"
                     /locus_tag="IW687_001216"
     CDS             7686..7886
                     /gene="rpmI"
                     /locus_tag="IW687_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0950490.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7939..8298
                     /gene="rplT"
                     /locus_tag="IW687_001217"
     CDS             7939..8298
                     /gene="rplT"
                     /locus_tag="IW687_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0950491.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8650..9888
                     /locus_tag="IW687_001218"
     CDS             8650..9888
                     /locus_tag="IW687_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0950492.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9936..12065
                     /locus_tag="IW687_001219"
     CDS             9936..12065
                     /locus_tag="IW687_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0950493.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            12040..12729
                     /locus_tag="IW687_001220"
     CDS             12040..12729
                     /locus_tag="IW687_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950494.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13137..13823)
                     /locus_tag="IW687_001221"
     CDS             complement(13137..13823)
                     /locus_tag="IW687_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0950495.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13959..14153)
                     /locus_tag="IW687_001222"
     CDS             complement(13959..14153)
                     /locus_tag="IW687_001222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950496.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14203..14643)
                     /locus_tag="IW687_001223"
     CDS             complement(14203..14643)
                     /locus_tag="IW687_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0950497.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14647..15492)
                     /locus_tag="IW687_001224"
     CDS             complement(14647..15492)
                     /locus_tag="IW687_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0950498.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15641..17059
                     /gene="pepV"
                     /locus_tag="IW687_001225"
     CDS             15641..17059
                     /gene="pepV"
                     /locus_tag="IW687_001225"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0950499.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17135..18337)
                     /locus_tag="IW687_001226"
     CDS             complement(17135..18337)
                     /locus_tag="IW687_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950500.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18324..20111)
                     /locus_tag="IW687_001227"
     CDS             complement(18324..20111)
                     /locus_tag="IW687_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950501.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20124..21077)
                     /locus_tag="IW687_001228"
     CDS             complement(20124..21077)
                     /locus_tag="IW687_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0950502.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21262..21612)
                     /locus_tag="IW687_001229"
     CDS             complement(21262..21612)
                     /locus_tag="IW687_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0950503.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21812..22891
                     /gene="pepA"
                     /locus_tag="IW687_001230"
     CDS             21812..22891
                     /gene="pepA"
                     /locus_tag="IW687_001230"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0950504.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            23034..23354
                     /locus_tag="IW687_001231"
     CDS             23034..23354
                     /locus_tag="IW687_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0950505.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23376..23840
                     /locus_tag="IW687_001232"
     CDS             23376..23840
                     /locus_tag="IW687_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0950506.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            24036..24641
                     /locus_tag="IW687_001233"
     CDS             24036..24641
                     /locus_tag="IW687_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0950507.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24680..25969)
                     /locus_tag="IW687_001234"
     CDS             complement(24680..25969)
                     /locus_tag="IW687_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0950508.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26397..26876
                     /gene="rlmH"
                     /locus_tag="IW687_001235"
     CDS             26397..26876
                     /gene="rlmH"
                     /locus_tag="IW687_001235"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0950509.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            27229..27543
                     /locus_tag="IW687_001236"
     CDS             27229..27543
                     /locus_tag="IW687_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0950510.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            27559..27945
                     /locus_tag="IW687_001237"
     CDS             27559..27945
                     /locus_tag="IW687_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0950511.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            27974..>29100
                     /locus_tag="IW687_001238"
     CDS             27974..>29100
                     /locus_tag="IW687_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0950512.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9205 a         5456 c         5887 g         8552 t
ORIGIN      
        1 tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttt tgcttccgcc
       61 gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc acgctctttt
      121 ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa tccttcactg
      181 gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg ccgaccatag
      241 tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt tcttctacgc
      301 cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt gcgccgagac
      361 aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg atttccatag
      421 aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc cagtcagcta
      481 aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag cttgttccgc
      541 ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg agcgtttctc
      601 ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct gtttgagtct
      661 cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata accaactctt
      721 gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata gcttcttggg
      781 ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga ggatattctt
      841 gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg ctgactgaac
      901 cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg ttgatttttt
      961 ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt agtgtaaacc
     1021 caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa gggttatgga
     1081 tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta gctagtgaaa
     1141 catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga catgtaacgg
     1201 cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc cagatttctg
     1261 gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg cggctccttc
     1321 atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga cgaccccagt
     1381 gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt ggatcaacgt
     1441 tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac tgttttttaa
     1501 gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat cgaccaaatc
     1561 agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt caggagcaat
     1621 caatggctca atggatagac ctggccaatc cagggcaagt aactcactga tagaatgctg
     1681 agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc agccaccata
     1741 acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat tggctagtgc
     1801 tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat tcaaggcttt
     1861 gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc catttgaact
     1921 gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt tttcttgtgc
     1981 atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt cccgaatatc
     2041 taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat attgtgcaga
     2101 ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt gatcgactgc
     2161 agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa tatagagttt
     2221 gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg caattcctgg
     2281 accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg tttcttgtac
     2341 ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat ccatcgtaaa
     2401 gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt ttaagctgtc
     2461 aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca tttttagtcc
     2521 gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg cttgtttcaa
     2581 ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg atgtttcgac
     2641 tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga tcaaaacgaa
     2701 aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt aagaacttaa
     2761 gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc aagctgaacc
     2821 agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct taagagcagt
     2881 attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc cagctgcggt
     2941 aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt ctttacgaaa
     3001 aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg tgccatctag
     3061 ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg tataaaatgc
     3121 atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat ttttcttacc
     3181 ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa acataggtat
     3241 gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt gcttgactga
     3301 tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg cgtccaccgc
     3361 cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat agattaagaa
     3421 aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt gtctgtgcca
     3481 acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag tatcctaatt
     3541 tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg acatctttga
     3601 cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa gcatcgatgt
     3661 tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc aagctgctag
     3721 tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa agctggacat
     3781 tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa gcacgagtga
     3841 ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc gtgctttcaa
     3901 tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg aataattttt
     3961 tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag gaagaaacca
     4021 gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt gcatagaaag
     4081 gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat atgatctttc
     4141 cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg taaaagtgat
     4201 ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat ttttttgtcc
     4261 actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca gaagtaaaac
     4321 taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg actttttttc
     4381 ttctttgact agaaaaaata caattgacac tactagatat tgggggtata tttaaaaaag
     4441 gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga gcgtgatcgt
     4501 tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg ctgaacactt
     4561 tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct tattttatgc
     4621 aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac gttttgcaag
     4681 catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt atgaagattc
     4741 actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag ttcgtgtgta
     4801 tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata aaaaacatcc
     4861 ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta tcgaaaaacg
     4921 tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt acaaagaatt
     4981 gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa tccaaatcga
     5041 tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct tgaaaatgat
     5101 cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta cagttgctca
     5161 aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac aaatgtcagg
     5221 aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt ggatcaatgt
     5281 acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca ttagtgacga
     5341 gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg attcttttga
     5401 tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata atcattatga
     5461 aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta ccacgccgaa
     5521 taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag acatgagtgt
     5581 atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc ctttaatcga
     5641 ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa caacgaacaa
     5701 gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta caggaacaaa
     5761 acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca agttgtcaga
     5821 aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag ttgaaatgac
     5881 gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt tgctgaataa
     5941 caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga ccgttccaac
     6001 tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt ccgttttcgg
     6061 atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta ttgcactgtg
     6121 gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc gatcacaagt
     6181 gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc gattgttgtg
     6241 aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt cgaaccggtt
     6301 aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc gctgtcttct
     6361 aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga agccatcgaa
     6421 gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt cgtacctgtt
     6481 aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc cagattggaa
     6541 aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc tgttttttgc
     6601 atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc gagatagctt
     6661 tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa ataaaactct
     6721 ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat gtgggttcac
     6781 ctcgtttttc tttagtatag caaagaagcg ggaatcactg caagaaagcc tgctaatcta
     6841 atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag attagttttt
     6901 gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc ttagttgata
     6961 atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg cgggttctgt
     7021 aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc tcataatatt
     7081 tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg tgcacgagag
     7141 ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc agaagcattg
     7201 aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc gaaaccacca
     7261 gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa agaccgcgaa
     7321 gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc tacgatcgac
     7381 ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa gggcgataaa
     7441 gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat tggtcaaaaa
     7501 gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca aaaagcgaaa
     7561 atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa gtaatttgaa
     7621 tgactaactg gctgttggac agccgatagt cggataacga aagattttga ggaggaaata
     7681 tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa cgtactggta
     7741 aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc aaaacaaaaa
     7801 aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc aaacgtattc
     7861 gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa gatttatttt
     7921 tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg taaacgtcgt
     7981 aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt atttaaaaca
     8041 gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg tcaaaagaaa
     8101 cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat gaatggcttg
     8161 agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa ccgtaaaatg
     8221 ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga ccaagcaaaa
     8281 gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac gaaaagttat
     8341 gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca ataacgtata
     8401 agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat ggtcatcctt
     8461 taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta gtttgcttat
     8521 cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt attttgttag
     8581 ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg atggtcggct
     8641 tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
     8701 aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
     8761 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
     8821 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
     8881 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
     8941 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat cttgatgtca
     9001 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
     9061 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
     9121 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
     9181 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
     9241 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
     9301 ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt tattctagca
     9361 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
     9421 cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt tggtttcttg
     9481 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
     9541 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
     9601 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
     9661 gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatt
     9721 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
     9781 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
     9841 ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
     9901 cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
     9961 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    10021 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    10081 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    10141 ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta gcaaaagtcg
    10201 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    10261 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    10321 tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    10381 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    10441 tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac atcacaaaat
    10501 tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt ttaccaaaaa
    10561 tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc aatgtttctt
    10621 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    10681 ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg tacttgagag
    10741 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    10801 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    10861 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    10921 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    10981 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    11041 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    11101 cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    11161 cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa gacatcgcag
    11221 agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    11281 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    11341 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    11401 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    11461 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    11521 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca catacttcaa
    11581 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    11641 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    11701 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    11761 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    11821 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    11881 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    11941 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    12001 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    12061 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    12121 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    12181 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    12241 tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc ctgtgaatca
    12301 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gatccgattg
    12361 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    12421 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    12481 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    12541 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    12601 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    12661 caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta tgatttagat
    12721 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacggt
    12781 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    12841 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaaag
    12901 gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca taagaagcta
    12961 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    13021 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    13081 ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    13141 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    13201 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    13261 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    13321 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    13381 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    13441 aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg ttctagtatt
    13501 ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg caggatcggt
    13561 ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc gatttttgaa
    13621 acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag ttggatcagt
    13681 gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt atcggagatt
    13741 ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc ataagcttgc
    13801 tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa tctattataa
    13861 cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa agccctgata
    13921 ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg tacctcttgg
    13981 atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt tttgtctttg
    14041 aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt atcgatgtaa
    14101 ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt cattatacga
    14161 actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat ttacatcatc
    14221 caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc tcagcttcac
    14281 taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag tttttaattt
    14341 tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg tatacttttg
    14401 gatagttggg tggatgctca ctttttctcc gtaatggatc atttctttat agctgcaatt
    14461 tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg gaaaccctag
    14521 atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc tgtgatgaat
    14581 aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg taaatttctc
    14641 catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc acttcggaag
    14701 tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct ggtctccaat
    14761 gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa actgggctaa
    14821 aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cctgttgcca tagcagcaga
    14881 acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat gacttttcaa
    14941 aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc gttgttcttg
    15001 tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta cagtttgttc
    15061 gggatagggt agcaaaagat gattagcaac aagtaaactt aaagcagagc catcaatcac
    15121 cagccattgt tcttttgatt gtttttctag tacttgtttg aaacgttcca aactcttaga
    15181 agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta cggaaagaaa
    15241 ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt gaagagcact
    15301 ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccactgtgta cggcagctaa
    15361 agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa cgatacgtcc
    15421 aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg actcttttca ctagttgttc
    15481 gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt tttagagaaa
    15541 aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta atttgtggta
    15601 tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg attggcaaaa
    15661 agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt tacgtgtcaa
    15721 cagcgagcgc gatgattcaa aagtaacacc agatgcacca tttggacctg gacctcgcga
    15781 cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa aaaatgtcga
    15841 taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga tatttggaca
    15901 catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac cagtaatcaa
    15961 ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta tggcagctta
    16021 ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag ttcgcttcgt
    16081 agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg aacatgaaga
    16141 agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg gtgaaaaagg
    16201 aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg tcttgaaatc
    16261 ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag cacttcaagt
    16321 accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca tcgaagcaaa
    16381 tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat tagttggtaa
    16441 aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct tggcattgtt
    16501 cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag cagctgctta
    16561 tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg aaaaaatggg
    16621 caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag aggaagcgaa
    16681 cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat tgcaaagtga
    16741 catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc gcgtaatgga
    16801 accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag tctatgaaga
    16861 ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg gacgtttgtt
    16921 gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa tgcatcaagc
    16981 aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg cagatgccat
    17041 ctatcgtttg gcgaaataaa aatactaaaa aactccggct atctctgaca gctggagttt
    17101 ttatgtttaa aaatgaatta gtacatcggt attcttaaaa gtaatttagt tcgtcttttg
    17161 atttctcagg agggatcaaa aaggcgaaag cactaagtat agcaaaactt gtgatgattt
    17221 gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt agctaaaata ttgtaccaaa
    17281 tatacggcgt tacagcagct aaaacaaaca aaactagaag ttggaacgga cgccagctat
    17341 ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt aatcaatagc aataatattg
    17401 ctacttttgg atacatcaat ctaatattgc ttttcagcat ctctaatcga tgcaacgggt
    17461 atttgtcgtt tccttccgtt cgaaagagga tttggttgat tgcatctttt acaatgtttt
    17521 tgttcaatat gatcgatgat aggatccatt tagccaccca tgtcaaacca tagcctaatc
    17581 cccaaaagat accggtaaaa acaaaactgc ctacttgttt gctcaatgaa ctgcgtttat
    17641 ccattaaata cttatttgtt acataataaa taatgatcac aggtatcccc caagagatga
    17701 ccggtgttgt caacaagtca aaaaagttag ctaaagaacc aatgacaaag aaatacaaga
    17761 ataattgttg gttggtgttg gagagttgtg gttttgttaa aaatagtaat acagacaata
    17821 aagtcaaaat ccaaatattt gaaaattgca tactgaagaa aaaggttgca gcatttccta
    17881 tagcaagcga aatcaacaag gctgtcgtta aatagatgtc gctacacttg gttaaaaagt
    17941 agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta aatcattcga atactcccca
    18001 aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata accatgccaa taacggggat
    18061 aatccgccat agacatcgcg ttctccaagg agttcaattc aggatctgct tttgtttttc
    18121 gtatcatcaa atgatcagta aaattatcta accgagtgcc tagcatattt ccttgattga
    18181 tagaaggata cattccttct ttttcaacca tctgtagaga tttttctgca ttcgattcaa
    18241 tagagtgctg cggaatgaaa aaagtaattg tcagtagtac gcaatagcta gcgatcaaga
    18301 gaacaaaagc aaaaaatatc tttttaaata gtttcatgag tatctcctat atctgttttc
    18361 attttttctc ttttatctag taaaaaagga gagaggatat aaacaggtag acctaatgct
    18421 aaaacaaaca cataccgtaa actaaaagcc acaggagtag caatcataat agtagcaaaa
    18481 acaagcatac cgggaagaaa aaggagtaaa taccgatatt ttttcttaaa gattgctaaa
    18541 agtgcactgc cgatagctaa ccacagtaat gtgccggaac caaagaaatc acgagcatcg
    18601 atgatttttt tcattcctga caatccagta agatgttcaa tcaaatctac ttgcttcata
    18661 ccataattat tcgtgtttac acgagtatct aaaaagccgt aactattttt tgtaccaggt
    18721 acccagaacc cgaacgtttc taaaatataa gcatctgtat agatttttat attacttggt
    18781 aaatttcttg cccatagtga tagaaattct tttttatgtg tattcaaaaa ctcattgtta
    18841 aaatttttat gccacttgat tgagtctgca agactaggag aatacgcttc ataatctttt
    18901 atcgggagta gctgattcat gaatgcttta tctttcttag acaagtcacc atccataaca
    18961 atagttctag aaatttgctg caaaggaaca cctaattgtt cagcagtttc tgtaggaata
    19021 gcataacgag ccatcaacgg gtggatgagt agtccagcaa tcactgtata acccagcacc
    19081 acttgaaaga atgcttgata ccttttttgt ttcaaaataa tcaagccgca aatcaaacct
    19141 gtgccggtta ctacataaat cccattattt cttagatagg tggtcagtaa agcaaggatg
    19201 attacgtaaa tctgattgag aatcgttggc ttttctcgtt gaaaaataaa gtcaaaaacc
    19261 gctaaggaaa ataaaaagca agcgatagag aacaaagtat ctttttgcat gttcaaggcg
    19321 tagattggaa aaaccggtgc aaatgcaaaa tagcataaac taatgagtac cggaaaaaaa
    19381 tggatgtttc tttttaacag ccagcaaaga gaataactta tcgctgaagc tgttatcaat
    19441 gtttgaaaaa tcgaaaataa aaatacaccg tgattgatcg tatcagttgg taaatcatga
    19501 acaaatagct tgaccagtaa ggtgaataag actggatgat gattgcttat aggaacatca
    19561 cccatagatt gggcgaccga acttaacgaa tcaggcaaaa ccactccagg atacaatgcc
    19621 aacagataag gaagccaaca cagaactaaa aagacagtaa agaataaaaa atattttctt
    19681 gaagagaaag cctgtgtgtt ttgagcagta aaaaatgttt ccagttttgg cattgataaa
    19741 taaatcgatt gtaaaaataa aaaagaatac acaaaaatgg agaaataaac aagaaagtaa
    19801 aaaatacttc catcagcaat cgttgtcttt gtatagtttg ctgagacata ctgaaattgt
    19861 atctgtctgc caatagaaag tgtaagagca aaagctgcag aaaaaaacaa tcgggcaata
    19921 gctgcaactt tgttttttat tgaaaacaac tctattccat attgcttaaa agcaagtagc
    19981 aaaaccatgg ctaaaccaat gattactgtt ggattcaaag ttcctagacc ataagaagta
    20041 aaaaaagcaa aacctgacgc aaataaagtt gcaaaaaaag taggaaatag attctccttt
    20101 ttcttatgca taaaaaaggc ctcctacttc atttctttat tttgatattc gtggtagaca
    20161 tggtataaat tcaattcata ttctttcttg gcatttgtga cgaccgtatc caaaatcaac
    20221 cctgttacta atgaaagaag ggctaaagtc atcaatccag tacttaaaat cgcagaaggg
    20281 atgcgtgtaa taaagcttgt cagcatatat tcacgaatca caggaatccc catgaccaat
    20341 ccaaataaaa agaagaagat ggtccagatc ccaaagaaaa gcaaaggttt ataatctttg
    20401 cacattttga tgatcatcat gatcactttg aatccgtcag agaatgtatt caattttgat
    20461 tcgctacctt caggacgatc gcgataatcg attggaatct caacgaactt gaatttttta
    20521 tctaaagcat gaatcgtcaa ttctgtttcg atttgaaaac cagaactcat aatagggaag
    20581 cttttgacaa aaattcgatt gaatcctcga tagccggtca tgacatcgcg aatttttgtt
    20641 ttatataagt aagtaattgt atttttaacc agattattcc caaaatcatg gaaaggacgc
    20701 ttattttcat caaaataggt tccatttgat agacgatcgc caatgaccat atctgcttcg
    20761 ccagaacgta atttctcaag caaaccatgt gcagcttctg caggataagt atcatcacca
    20821 tctaccatca aataataatc ggcatcaata tcaaaaaaca tttgacggat tacattccct
    20881 tttccttgac gtggttcttt ctttacgatt gctccgcctt gaacggccaa ttcgtatgtt
    20941 cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc ccttttaaaa
    21001 tcttcaataa cagttgaaat tgtcgcctct tcgttataac aaggaatgag cacggcaatt
    21061 ttttcacgtg attccatttg tcactctcct tatataataa aactaatctt atttcaataa
    21121 taccgtataa taactacaaa tactagctat tccaagagga aatagcccta tttttataaa
    21181 aatcacccac tataaaagaa ggataggata cctctctttt agatagtggg tgcgtattcg
    21241 ttatttcatt tttttgacgt attaatctat tgcgcccttt tttcttgaaa tgtctaatac
    21301 ggttcctgta tgagcatctg ctaaaaattc ataataaaca gtttcgccat cttctatccg
    21361 ggaaagccca ccccgataag cactcgaccg aacggcgaat ttccgaatag gttctttttc
    21421 aaaagagatc caagcacctt cgattgcgcc ttcttttaaa aaagatgttt tgatattttc
    21481 aagcacttgg tctgctgata acactttttt gcgatgatac caaatcgttg aagctacccc
    21541 actcaataat cctagaccga tcccaacggc taacccgcct ttaaaatagc ttaattcgct
    21601 ttcttcatac atgtggatat acctccttct tctcacttgc tactctttgt gtttatttta
    21661 ccacactctc atattttctt tgaaataaat aaccgctacc agaaaaaata agctataata
    21721 aatacgatcg gtctgcagaa aaaatcatat tttatccgca gatcaatata agtctgagag
    21781 gagtttagtc tgaaatttag acgaatagca tatggaagaa aaaacatttc aacgaatcaa
    21841 agagttaaca gaattacaag gaacaagcgg ttttgaacaa gacgttcgcg cctatatgga
    21901 aagtcatatg gaaccgctgg tagatgagtt acagttagat ggtttgggcg gcatttttgg
    21961 gctgcgccac catgaagaag cggatgcacc tcgtgtaatg gtcgcagcac atatggatga
    22021 agtcggattc atgctgacac aaatccaaga aaacgggctt tttaaagtcg tacctctagg
    22081 cggatggaac ccttacgttg tttctgctca acgttttaca cttaaaacct ctactggtaa
    22141 gaattatcca tgtatttctt cttccgttcc cccacattta ttacgaggaa caagcggaca
    22201 aaaatcagta gaagtaacag atattttgtt cgatgctggg tttgaatcaa gagaagaggc
    22261 aatgagttac ggtgttcttc caggagacac aatcgttcct tacgctgaaa cgatcaaaac
    22321 agccaacggg aagaatatca tcagcaaatc gtgggacaac cgctatggct gtacaatggt
    22381 attagaagct ttagaagcgt tacaaaatga aacattaggt catacattga ttgcaggcgc
    22441 gaatgtccaa gaagaagtag gcttgcgtgg ttctaaagct tctgtcaata aattcaagcc
    22501 agatctgttc tttgcagtag actgctctgc agcagatgac acagtgacga aaaatggcac
    22561 attcggacat ttaggagaag gtacattgat gcgtatccaa gatccaggtt tgatcatgct
    22621 tcctcgtttg cgtgagtact tgctagatat cgctgaaaca aacaacatcc cttatcaata
    22681 ctttgtttca aaaggcggta cagatgcagg tgctgctcat acacaaaatg aaggaatccc
    22741 aagtacagtg attggcgtag tgggacgtta tatccatacg catcaaacga tgttcagtat
    22801 tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca ttgaaaggtt tagataaatc
    22861 aacagtgaac acgatcgtat acgggaaata acgcgatagt gaaactataa aatctggcaa
    22921 aagcaacctt ttgtcagatt ttttttataa aaagaggatc aatgtttgtt agttcgaaag
    22981 aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggaaaa agaatgatta
    23041 tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaaaagt gtatttttct
    23101 ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct gaaatcgaaa
    23161 atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata gatgtagccg
    23221 ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga aaagaattag
    23281 gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc ctagaaagtg
    23341 taataaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat aagaacattt
    23401 tagtaggtat tgacggtagt gaacaagcac ttgctgcttt caaaaaagcg ttagaagtgg
    23461 ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa ttttataatt
    23521 ttatgggcta tgctcctatt gatcaaaatc tagtcgatca gcaaactgaa acagccaagc
    23581 aaatgatcga agaatgtaaa gaatatggaa aatctgttga ttacacaaat atagaaggaa
    23641 ttattgcata cggctctgtt cgtgaatcca tggcagtcaa attgccaaaa aaatatgcaa
    23701 tcgatctaat catggttggg cagtctggac taaatgcagt cgaacgcttt atgacaggaa
    23761 gtgtagccag ctacgtcata cgtcattcac cttgtgatgt cttgatcgtt tctgacgaag
    23821 aagaacagcc aagtaattaa gcaagtggaa cttgtataac ctttttaatc aaacagtaaa
    23881 gggactgtga caaaagtctt gtcacagtcc cttgttacga ataaatggtg ttccagaaga
    23941 agcttcttcg gaaataaagc atgaaaaaaa tctcatgcag tgaatgcaat tttttgttaa
    24001 gataaatctc agcaaaggat aatggaggaa agaacatgat ttttgcttat aacaaagcac
    24061 acgtcggaga cactttgatg gttatcatag ccgacgataa aggaacagaa aacactgtcg
    24121 aacgtaaatg gaatgttgct cggatcaaag atgaacaagg tcaagttgtt gcttggaact
    24181 ttttccatat ctccgatcat ctaacaattg aagggaacgg ccaagtaagc ttaacagatg
    24241 aagaattagc gaaattgaat cgattgatta aagaagcagg attcgaagaa cagcttgaag
    24301 ctgatcatga accgaagatc gttgtcgggt atgtaaaaac atgcgtcccg catcctgact
    24361 ctgaccattt atcgattact gagacagaag tggacaatgg gacagtattg caaattgttt
    24421 gcggtgcacc aaatatcgaa gctggacaaa aagttgtcgt tgcaaaacca ggagccatga
    24481 tgccagatgg aatgatgatc tggccaggaa ctttgcgagg ggtagccagc tatggaatga
    24541 tttgttctgc tcgtgaattg catttgccaa atgctccaga aaaaaaagga atcttagtat
    24601 tgcctgagga tgccgtagtc ggagaagtat ttccaaaata aagaggttgt gacaagtctt
    24661 tgtcacaacc tctttattat taattttcac tattttgatt ttgttttaat gccgattgat
    24721 cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa gtgattttga
    24781 cagaatctcc cacttttttc ttatacaaag cagattgaag ttctacacca gaagaaactt
    24841 ctttgtcatc gatcttagta atgacgtcgt attgtttcaa cccagctttt tctgccggtg
    24901 tagctgtttg gacatttgtc acgatgacac cgttagttac agatgaagga atcttcaaga
    24961 tttgttcttg ttgctgagta gaaacggcag ataagtcgac cattgtgata cctagagctg
    25021 gacgtgtcac ttttccgtct ttttccagtt ggttgatgat attcactaca tcgttacttg
    25081 ggatggcaaa tcccattcct tctacgctta cattagaagt cgattcagaa gtacttgcga
    25141 tcttacttga gttgataccg ataacttgtc cttcgatatt cactaaagga ccaccagaat
    25201 ttcccgggtt gatcgctgca tctgtttgga tcgcattgat attgactgtt tcgttcgatt
    25261 cattcgtact tgttacttgt cggttcaagg aagaaatgat tcctgaagtg acagaattcg
    25321 catattcaga tcctaatggg gaaccaatcg caatcgcagg ttcaccaacc ttcaaagcac
    25381 ttgagtcccc aaaagaggca accgtttcga ctttgtctga attgatcttc aacaccgcta
    25441 aatctgaata ggcatctgtt ccaactaatt cagcttttac cttcgtaccg tctttcatca
    25501 tgacttccaa accttgctgt ccatctacta cgtgattgtt tgtgactaca taagcagtat
    25561 tcccgtcttt tttgtagatt acgccgcttc cttcactata agcttccaaa ttactatcat
    25621 ctgagctgct actttcttct tgctgcccga ataatccgcc aaaaccgctt gattggtttt
    25681 gactttgtaa gttgataaca gaaacgacag cgtcttgaac tttatctacc gctttggtga
    25741 tatcagaatc tacattgact ttgacatttt ccacaactgt ttcaccggca gaattttgat
    25801 tgccgcttgt agcagtagaa ctattgttgc cagtgcccat cgctgcataa aaaatgccag
    25861 ctgtcagtag tcctccaacg atccctccaa caagccccag acccaactta cgccagaggc
    25921 tattattttt attttttttc atctttggtg tcacattttt tctatccatg tgttcctcct
    25981 ccaattacat tcatttatct actgttagta tagtcttatt tgttgacttt agtctacttt
    26041 gagactttga actttttatg aaaaacttta ggaaaaatca ttgttttcag atgaaaatat
    26101 ttgattttct ttttccacag atattgtgta aaactacgcg gataggattg gaaaaataag
    26161 ggataaaaca tatccacagc ctgtgtataa agtggataaa ttcttataaa acgtatgttt
    26221 gcctaatgaa tgtgatgaaa taaggtaaaa caacgctgtt ttctgtggat aaacctgtgg
    26281 atgttgtgca taactgttgg ggaaatcttt ttaacaaaaa tagtcaaaat aaacatatag
    26341 acgaaaaaat tgattataat aaacaaagtg agcgtgaaga tcaaaggaga gaaaacatga
    26401 atatcaaaat tatttcagta ggaaaattaa aagaaaaata cttgatccaa ggtatcaatg
    26461 agtatgtcaa acggctgaat gcctatgcga aaatcgaatt gatcgaagtt cctgacgaaa
    26521 aagccccgga gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa gaaggagaac
    26581 ggatcttagc gaaaatcaaa gaacaagaat acgtctatgg cttagcaatt gaagggaaaa
    26641 atcccacaag cgaatctttt gctaaacaaa tcgaccaatt aggtatccaa ggaaaaagcc
    26701 accttgtttt tgttatcggc ggttctttag gactcagcga agccgtcatg aaaagaagca
    26761 atgctcagat ctcttttggc aaaatgacgt atccacatca attgatgcgc ctgatcctcg
    26821 tcgagcaaat ctaccgtgcc ttccggatca acgcgggggc tccataccac aaataatgat
    26881 attttttaag agaaaaatag cataaaaatc tagttatccg cataaaaact ggacttatca
    26941 cactttatca aggtcaaaac cactcaattt actactaatt tactacttat gaatgagctt
    27001 tgatacgacg atttatcctt gaaaagtgaa gatataaaga tacttccaat aaaatttgaa
    27061 tatttaatag gtagacactt caaaaaatga ggtgtctatt tttttacccg attttgaaag
    27121 gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca ctctttaaga
    27181 ccatcaaaca ttcattcagc caataaaaaa gaaaggatag gtaaaaatat ggaacttaaa
    27241 tttgtgattc ccaacatgga aaaaacattc ggcaatttag aatttgctgg cgaggataaa
    27301 gtcgttcagc gaagaatcaa cggacggcta actgtcttat caagaagcta taatctctat
    27361 tctgatgttc aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc tggcgaaaaa
    27421 catttcggct ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc agagggctac
    27481 aaaatcggca ctcgtggttt tacaaattac cttttacatg ctgacgacat gataaaagaa
    27541 taaagaaaga gaggaaaaat gatgagatta gcaaatggca ttgtattaga taaagacacg
    27601 acttttggag aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca aaatgaagac
    27661 gggtcggttt cagatgaaat caaggaacgt acctatgact taaaatccaa aggacaagga
    27721 cgcatgattc aagtaagtat tcctgccagc gtgcctttga aagagtttga ttataacgca
    27781 cgggtggaac ttatcaatcc cattgcggac accgttgcta ctgccaccta tcaaggagca
    27841 gatgttgact ggtatatcaa ggcagacgat attgtgctga caaaggattc tagttcattc
    27901 aaagctcaac cacaagcaaa gaaagaaccg acacaagaca aatagtcgct aggtagaaag
    27961 gagacttttt cgcatgaaac agcgtggtaa aaggattcgc ccatctggta aagatttagt
    28021 ctttcatttt acgatagcgt cactcctgcc tgttttcctg ctggttgtcg gactgtttca
    28081 tgtgaagaca atccagcaga tcaactggca ggattttaac ctatcacaag cagataagat
    28141 tgacattccc tatttaatta tcagtttcag tgtcgcaatt cttatctgct tgctggtagc
    28201 gtttgtattc aaacgggttc gctatgatac ggttaaacaa ctttaccacc gtcaaaaact
    28261 ggcaaagatg atacttgaaa acaagtggta tgaatctgaa caggtcaaaa cagagggttt
    28321 ctttaaagat agtgctggtc gtacaaagga aaagataacc tacttcccta aaatgtatta
    28381 tcgacttaaa aatggcttga tacagatacg ggtggaaatc acgctgggaa aatatcaaga
    28441 ccaactctta cacttggaaa agaaattaga gagtggcttg tactgtgagc tgacggataa
    28501 agagttaaag gattcctatg tggaatatac tttgctctat gacaccatag ccagtcgtat
    28561 ttctattgat gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa acgtatggtg
    28621 ggaatatgat aagctccctc atatgttgat tgctggtggt acaggtggcg gtaaaactta
    28681 ctttatactg acactgattg aagccttgct tcatacagat tcaaaactgt atattcttga
    28741 cccgaaaaat gctgaccttg cggacttagg ttctgtgatg gcaaatgtct actatagaaa
    28801 agaagacttg ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac gtagtgagga
    28861 aatgaagcag atgaagaact ataagactgg caaaaattat gcttacttag gtctcccggc
    28921 acacttctta atctttgatg aatacgtcgc tttcatggaa atgctgggaa caaaagaaaa
    28981 caccgcagtt atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag ctggcttctt
    29041 tctaatactg gcttgtcaac gtccagacgc aaaatattta ggcgacggaa tccgtgatca
//
LOCUS       DACELF010000030        27999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.030, whole genome shotgun sequence.
ACCESSION   DACELF010000030 DACELF010000000
VERSION     DACELF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.030"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW687_001239"
     CDS             complement(5..586)
                     /locus_tag="IW687_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0950513.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW687_001240"
     CDS             complement(769..1395)
                     /locus_tag="IW687_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0950514.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW687_001241"
     CDS             complement(1417..2748)
                     /locus_tag="IW687_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0950515.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW687_001242"
     CDS             complement(2903..3304)
                     /locus_tag="IW687_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0950516.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW687_001243"
     CDS             complement(3390..3596)
                     /locus_tag="IW687_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950517.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW687_001244"
     CDS             complement(3691..5193)
                     /locus_tag="IW687_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0950518.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW687_001245"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW687_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0950519.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW687_001246"
     CDS             complement(5764..6381)
                     /locus_tag="IW687_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950520.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW687_001247"
     CDS             6602..7975
                     /locus_tag="IW687_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0950521.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW687_001248"
     CDS             8050..9162
                     /locus_tag="IW687_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0950522.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW687_001249"
     CDS             complement(9234..10871)
                     /locus_tag="IW687_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0950523.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW687_001250"
     CDS             complement(11039..11647)
                     /locus_tag="IW687_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0950524.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW687_001251"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW687_001251"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0950525.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IW687_001252"
     CDS             complement(13201..13953)
                     /locus_tag="IW687_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950526.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IW687_001253"
     CDS             complement(14094..15044)
                     /locus_tag="IW687_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950527.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IW687_001254"
     CDS             complement(15045..15884)
                     /locus_tag="IW687_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0950528.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IW687_001255"
     CDS             complement(15881..16627)
                     /locus_tag="IW687_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950529.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IW687_001256"
     CDS             16865..18235
                     /locus_tag="IW687_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0950530.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IW687_001257"
     CDS             complement(18290..19186)
                     /locus_tag="IW687_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0950531.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IW687_001258"
     CDS             complement(19467..20144)
                     /locus_tag="IW687_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0950532.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IW687_001259"
     CDS             complement(20235..21140)
                     /locus_tag="IW687_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0950533.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IW687_001260"
     CDS             21315..22769
                     /locus_tag="IW687_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0950534.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IW687_001261"
     CDS             22785..23816
                     /locus_tag="IW687_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0950535.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IW687_001262"
     CDS             23823..25010
                     /locus_tag="IW687_001262"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0950536.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IW687_001263"
     CDS             25166..26548
                     /locus_tag="IW687_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0950537.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="IW687_001264"
     CDS             26520..26906
                     /locus_tag="IW687_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950538.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="IW687_001265"
     CDS             complement(27106..27774)
                     /locus_tag="IW687_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0950539.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7795 a         5725 c         5146 g         9333 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt ttgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacg
//
LOCUS       DACELF010000031        27800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.031, whole genome shotgun sequence.
ACCESSION   DACELF010000031 DACELF010000000
VERSION     DACELF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.031"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(240..1541)
                     /locus_tag="IW687_001266"
     CDS             complement(240..1541)
                     /locus_tag="IW687_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0950540.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDQYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(1553..2170)
                     /locus_tag="IW687_001267"
     CDS             complement(1553..2170)
                     /locus_tag="IW687_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950541.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            2211..2903
                     /locus_tag="IW687_001268"
     CDS             2211..2903
                     /locus_tag="IW687_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0950542.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
     gene            3574..4173
                     /locus_tag="IW687_001269"
     CDS             3574..4173
                     /locus_tag="IW687_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0950543.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSVGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQVHDFSNYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4222..4923
                     /locus_tag="IW687_001270"
     CDS             4222..4923
                     /locus_tag="IW687_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAQ0950544.1"
                     /translation="MNEEKRPNFPDKYQLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEEIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLVETSKRINRIVAAEDA
                     LFPGEIRTGGVEVSTIQGRYGPPMLTEGEVKKQYDEIINQNTSETEKALRLFLFISKK
                     QIFWDGNKRTALITANSVMFNQGAGLISIPEKIFSKFNELLSDFYNSNNHMYYEKIKK
                     YMYLNCIFGIDFIGK"
     gene            4945..8709
                     /locus_tag="IW687_001271"
     CDS             4945..8709
                     /locus_tag="IW687_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950545.1"
                     /translation="MKMGSSKLEDAENSEPEEVEEKTIDVEFEHLTPNDSMDLTAYKE
                     ALDYGIKNRMTNIALSGPYGAGKSSVLEAYKKNHTDKKFMHISLSHFEGSKEIDTKIL
                     EGKIINQLLHQIEFKKIPRTIFKAKENQSKFKMIFATSIVFLFITSGIITFQFSKWKK
                     YYTSFFSSEKFASWFTGNTFHLISLLVFTSILVFLIYNFINLQVNQQVMKKIILKGNE
                     IEIFKDSHESYFDKFMNDVIYLFINSGKDIIVFEDLDRFDNATIYEKLHEINTLVNRR
                     LVLKNKKKLTFIYLIKDDTFKSKDRTKFFDLLIPVVPVVDSSNSFDQLLSIFERNKVK
                     KDFNINIIRKISFYIDDMRLLKNINNEYIIYKSRLRSISLDCNKLIAMITYKNLFPED
                     FSLFQSGVGYINSVIKSKERILSKEIQKLSDEIELLNSSINIAKKEHLNDIDELDALY
                     LKLDNDGYFSVEDKKEDEFTTRKDFIRAIKDNNFNIIKYTPRSGSYRLEWHRSEINIS
                     GKFKELTNNDEYRLRLEAINNKRIIDMNQNKIINLEIEKKNRMNSSLSEILSNINNNF
                     FIDTNYFSEEFHYLFKSQYFPLIVFLLREGLIDENYGDYITYFYENSLKKDDKEFLRS
                     AYDRNPKELNYKLQNSNQIVTNLTPGDITTYDIKNIDLAAYLVSIYPENNLYLKSVID
                     VMKSCEDNSYILGLFEKIKNSGDVEKFTNISNDFWPTIFADIISKSDKNDDILEFLYV
                     ISSFAKIQFLKVNNEDNLLTNYISERRFIHPLILSKEQQEVLLEKFKKIGIKFHDLKK
                     SNADIDSLKAVINSRMIDISESNLEQILQIYDIKYSRDEFKYSNITLFYENNPDNIYE
                     YLAKEKINEYIRVYLKFGMETLKENSNVFVEILNSEKLNKELGFELIKKTNLANQIED
                     LKYVINTDYWNSLLIAEHIKIDERNIVSYYKEADNDFSEQLVTAINKTTVKITFSKDN
                     LSDKTREELWETIVHNNQLDNRQYISMLKSLGFYWRNGFKLSVSSLKIKQLIYAKIIR
                     NTKKNLNEILNNHKVNLVDFVQVDIDNFCNIFIDEDIYQFSVIKDLLMEKELVDSKKK
                     RIVDISKQDISIQNLNVSYRIQKYILENKFEDNDFSYIIEEYSKFNNLVKSTIYIKAM
                     SNIERIVQEKVSINIELLLEMLSDEQISERKILFSYYIQLLDDKDVIKYVRSLNFPEE
                     FILVLKKRRPKFENSSINKRILEDYRRRDWITKIYDRGNYIKVQGRMVLK"
     gene            9562..9795
                     /locus_tag="IW687_001272"
     CDS             9562..9795
                     /locus_tag="IW687_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950546.1"
                     /translation="MNENEGDRKVEVLINDLLAERQMSLRELARLSGIEPSNLSNLAN
                     GKRQKIYLEHIERIADALEIDDITRILKLSRNV"
     gene            complement(9772..9906)
                     /locus_tag="IW687_001273"
     CDS             complement(9772..9906)
                     /locus_tag="IW687_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950547.1"
                     /translation="MYHRIRNLREDKDLNQEQLAKLLNISQTTYSRYESGKLDIPTQL
                     "
     gene            10642..13686
                     /locus_tag="IW687_001274"
     CDS             10642..13686
                     /locus_tag="IW687_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008381780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0950548.1"
                     /translation="MFQEKIFNPAILHENRLSPRTSFFSYETVAAAMQGDPQASFGYQ
                     SLNGLWDFYLGEAENQVVYEQKIEVPAHWQFQGHGKPIYTNVQYPFPIDPPFVAMENP
                     TAFYRKAFIVEDINLEHVLHFEGVESVFEVHVNDQYVGYSTGSRLVSEFDITPYLQKG
                     KNVLSVKVKQWSAMSYVEDQDMWWLGGIFREVYLFSRKPQSIQNVTSQADLCNQYKDG
                     FLAVSLFFQPTTTEEPEERTIEIELLDQENVTVLKKAEKRLGGAKANWTISATIEEII
                     PWNAEEPYLYHLLVKQYQGEELLEVIPLQVGFRQISITDGIFQINGKRVVLKGVNRHE
                     WHCESGRSLTVETMEQDVQMMKAFNINAVRCSHYPADTKFYHLCDRYGLYVIDEADIE
                     THGMDITQRINELSSSEEWLPAYLDRAKRMVEKNRNHPSIIFWSLGNESGYGENHLKM
                     AQLIKKLDPSRLVHYEGEARHLKALGEWQACQASDVISTMYTSVGEMAEQGERSLTQP
                     HILCEYGHAMGNGPGGLSEYLELFYRYPRLQGGFIWEWIDHGIKSIDNVSGETYYKYG
                     GDFAEEIHDGNFVIDGLLFPDRTPSPALFEYKKVIQPVKITFQLSEQACRITNRFNFR
                     NLASLQAVLQIKEDDQVIFLQELPRIDLPPQEETTIYLPEEATLSLAGRAQAVVTLFF
                     FEESAPDFSLEHAVAWEQAVIDQPVKSVQIPKANAFHCSETADELRITLAEQTYIFHK
                     KTGTLSQWLIEGQALLTEPLEMNFWRAPTDNDLLGSEEFGSASIAKEWEAFRIRHLKE
                     RVLAFTYEQRLDEIKVEIRRNSMPISLDWGIELSFTYRLSLAGDIRVEVQGTPFGNGP
                     KTLPRIGVQLMIDKQHQNVQWLGLGPHETYPDSCQSGFFDWWERTVPEMHTPYVRPQE
                     NGNHMKTKFFRLSKPSGGGLTISSKSLNFSVHHYTQNNLEQATHTNQLETSDAVAVNI
                     DYGVQGLGSASCGPGILPKYLLQNQAFAFDFQMNYQKEVEGA"
     gene            13689..14984
                     /locus_tag="IW687_001275"
     CDS             13689..14984
                     /locus_tag="IW687_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0950549.1"
                     /translation="MKKIALCMAMIGLGGLAACGNGSDTPEREGGEQNGAEEITIWAW
                     DPKFNIAAFELAESYHQEENPDFKINVTEISQQDSVQKMNTIFSSGSTQGLPNIVLIE
                     DFRARNFLEVFPEYFVPLTEYINVDDFMDYKIEVSSIDGEQYGMPFDSGVAGVYLRKS
                     IIEEAGLELSDFTNITWQTFNELGIRVKEATGKPLLSIDMNDLGHLRMMMHSSGYWYT
                     AEDGETPFIDGNPSLKQGLEDLKMLLDSGVMTTHNGWDQMLAGFNSGEVVGVLQGNWI
                     TPSVKAEESQSGDWAVVPVPKQESIEGSVNATNLGGSSIYVLDIDGKEEALSYIADVF
                     GKNIAFYEELIQTVGAIGSYVPASQGDAYHAPDPFFDDQAIYQDFMNWANEIPSVNFG
                     KNTYALEDIMNQGFLDYVNGADLDEVLKNTQMIADNQLN"
     gene            15007..15888
                     /locus_tag="IW687_001276"
     CDS             15007..15888
                     /locus_tag="IW687_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950550.1"
                     /translation="MKNKLFYKKGVMTGWLFIAIPTLLIFFFSFYPMIQAFLLSLQSG
                     SGTGLTFSGLANYRRLFSDQTFLTATKNTLIFLGFQVPVMVLLAMFFSVQLNQKTLKL
                     KGLFRTIIFLPAVTSLVAYSMVFKSLFATNGVVNALLVRLSIVSEPIAFFSSPFWAKV
                     IIILAITWRWTGYNMIFFLSALQNIDPAIYEAARIDGASPTKQFFKITVPLLKPVILF
                     SAVTSTIGTLQLFDEPMNLTQGGPGNATSTISQYIYNLSFRYTPDFGYASAVSYAIVV
                     MVIFFSMIQFKIGGEDK"
     gene            15885..16700
                     /locus_tag="IW687_001277"
     CDS             15885..16700
                     /locus_tag="IW687_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0950551.1"
                     /translation="MKKVGTYVFLGVFTILFAFPFYFMLVSITNPTVNIMQGKLLPST
                     HLLENWRNLVATYDLKQVFFNSARIALLQTILSVTIASLAGYGFEIYQSRLKNMLFNM
                     LLLSMMLPFAATMIPLFRMFGSFSTTIPFIGLDTVASVILPTLSTAFLIFFFRQNTKM
                     FSKSLLEAGRMDGLSELGLFFRIYMPTMKTTYAAAAIITFINSWNSYLWPLITLQSPE
                     KQTFPLMISAIGAANNPDYGLMMLAIVIATLPTMVVFFSLQKYFVAGMLGSVK"
     gene            17375..18367
                     /locus_tag="IW687_001278"
     CDS             17375..18367
                     /locus_tag="IW687_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950552.1"
                     /translation="MYWEEIELEKTLGPGVEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPAMRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            18383..19267
                     /locus_tag="IW687_001279"
     CDS             18383..19267
                     /locus_tag="IW687_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0950553.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTTQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            19316..20923
                     /locus_tag="IW687_001280"
     CDS             19316..20923
                     /locus_tag="IW687_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0950554.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSTSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            20924..22408
                     /locus_tag="IW687_001281"
     CDS             20924..22408
                     /locus_tag="IW687_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0950555.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDAYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVDWETGQMIVDNFHEMD
                     FGMDFYAPQTTTDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLTYDTAKQVFELDRQKLGRELTGAEEDPLNVRKVEIPLIDNQLVLEIFRDTS
                     SVEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            22584..23576
                     /locus_tag="IW687_001282"
     CDS             22584..23576
                     /locus_tag="IW687_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0950556.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIGMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGGTTKPLVRNDFA"
     gene            23628..24497
                     /locus_tag="IW687_001283"
     CDS             23628..24497
                     /locus_tag="IW687_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0950557.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMKQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVTGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWAIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQAFKEKMGSYVDTPALEEYLLTPMLADDAGL
                     IGCLALAIKKLEQ"
     gene            complement(24939..25478)
                     /locus_tag="IW687_001284"
     CDS             complement(24939..25478)
                     /locus_tag="IW687_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HAQ0950558.1"
                     /translation="MKRYIALLRGINVGGKNKISMKELKAGFEELGFRDVVTYLNSGN
                     VIFSSEGDDPQALAEMIKTMVKRDFSFDIAIYIIRQSELQERLAHIPDWSGTEDKTIY
                     DNLIFLMPSLTYEVLSEKLGAPNANLEQVFPYKDVVFWSYIRKDYQKTNWWKKTASTS
                     VGKQITIRTINTVRKIAKM"
     gene            25477..25674
                     /locus_tag="IW687_001285"
     CDS             25477..25674
                     /locus_tag="IW687_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950559.1"
                     /translation="MASPSFKTLLLADYRMDGAEAQSWLLSYPKYVSEKRLMQAYWWS
                     GRGVRIFALLISKNEETAYRW"
     gene            complement(25753..26007)
                     /locus_tag="IW687_001286"
                     /pseudo
     CDS             complement(25753..26007)
                     /locus_tag="IW687_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292166.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
     gene            25996..26454
                     /locus_tag="IW687_001287"
                     /pseudo
     CDS             25996..26454
                     /locus_tag="IW687_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
     gene            26451..27383
                     /locus_tag="IW687_001288"
     CDS             26451..27383
                     /locus_tag="IW687_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0950560.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            27628..27744
                     /locus_tag="IW687_001289"
     CDS             27628..27744
                     /locus_tag="IW687_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0950561.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         9081 a         4641 c         5655 g         8423 t
ORIGIN      
        1 attgtaggtt tggggagacg gatgtttttt ggaccttttt caacacatcc ctgactcttc
       61 cgacattgaa gaagaattaa tattcctaaa gatcacttaa tccgttgctt attctgccaa
      121 tcgaatatct ctttacctat acttttttat aaaaaatagg ttgatgggaa accctaagat
      181 ttcccatcaa cactaaatag tatagaacca tattttcgtt acaaggttaa gaatgacgac
      241 tatagttttt catctactaa ttttgtttta ttgctctcca tgactttaaa aaatggatag
      301 tagatgaaga tgctgatgac gatcaacagt aggcaaaaga gaatattgcg ccagtccata
      361 cttgaaagat aagcttgggc aaaaaaaggt gtgaaggaag gatcgacgat aaatccaatc
      421 ccaatccatc ctagttgctg agcaaagtag gtcagcaata atgaaatgat aggcgtaatc
      481 aaaaatggaa ttgccaaaat agggttaaaa acgataggta atccgaaaat cactggttcg
      541 ttgatggaac agattccagg aaccaatgac aattttccaa ttgtgcgata ttcgggtact
      601 ttgcttcgca tcatcaataa aactaaaccc aaagtaccac cggctccacc aagtacagaa
      661 atacggaaca tttgtaagtt cattaagtgt gtcatggtct caccatttgc cagttgttca
      721 gcattcaaac cagtttgagc cattcccaat gtaaagacga tagggaaaat aattgacgag
      781 ccattgatac caaatagcca taggatgtta cccaaagtca cgatcaataa gaaaccacct
      841 agactgccag cgatgttcgt tgctggagtc aggacttcca tcactgcttc agggaagatt
      901 ttgtttgttg ctgccaagaa aatcagattg atgccataaa agatgaatat atttgtcagc
      961 agtggaacga gcatattgat aaaggttgct accatgggtg gaactgtatc tgggagtttc
     1021 aacttaatat tttttacttc aaatagtcgg ttgatttcca ctactagcaa gccgatgata
     1081 attgcgacga acaatccgtt tgtcccaagg aaattaagat tgatggcacc ttcttccact
     1141 ggtgtacaaa ccattagata aatgacagat gcaaccatgc cattcatgga aggattaatt
     1201 ttgtattcat tggcaaggga aaatgcaata ccaaaaacac taatgaatcc gatgattccc
     1261 attgtcaaat tataaggggc tgtgatgaga tcataatttg ctacggcaaa atctttccag
     1321 ctggccataa atttcatgaa aatattagct gtatctggat tgtattgatc aatattgatg
     1381 ggtggatttg caaaaatcag aaaaaaagaa ccgataacaa taaagggtag gccaaacatc
     1441 ataccgctgg atatagcttt taggtggcgc tgattgccta tcttggctgc gatggggcta
     1501 agtatgtcat tcagtttcat gataaacgca gaattttcca tataacttcc tcctatactt
     1561 gccaaggatt aaatgaaaac gtacttggca cgacgtgatc tggatcatag gtctcgataa
     1621 ttttttcgta ttgttccttg tagtcattat caacttttgc ttgggggatc actttacttg
     1681 cttcatgcaa aaagtgttct gaaccacgcc ccattgcctg tccccgaaca gtatgtttat
     1741 ccaacgcaat atcaggaatt tctggaatat atcccatagc gaaactcttg ataacaatgt
     1801 tcttaagtaa atccgatgaa cgatcttttt cagattggca taggtaacga atcgcgtgaa
     1861 aaaaatacat cgcacgatcg gattcattgt attggaattc tttgcgcatg gcgtttaagt
     1921 tattgatcat gatggcggcc ataggattgc ccatgcccac atcctcaaca ctgatcgcca
     1981 atagccttct ccagagtttc tcttcaaatt gtggtgaggt gatatacatt tcataagcaa
     2041 attcacatgc ttctttttct ttgcctcgac gaatcgattt ctgtaatgct gagatagctt
     2101 catctccacg taagccatta cgggtagtca tttttgacca cggatcttga ataaatttcg
     2161 tttctgtcat gatataatac tcctattaga taaaatttga ggtgaatctt atggatgttg
     2221 aaatgcttat agataaatac caactgaatg actcagaagc tgaagtctta cgatttatga
     2281 aaaaaaatcg tcagtcttta aaaaatattg ggattcgtga cgttgccaaa gcatcgtatg
     2341 tttctactgc aacgattatc aatatggcga agaaaattgg atattccggt tatagtgagt
     2401 tggtatttgc tctaggaaac agccaatctg acattacgat gccaagcaca ttctccaatg
     2461 aggagaagga agcattcata catttaatgt ctgcgtatcg cgataaacgg attatgattc
     2521 ttggctcagg tttttcgcaa aatctagcca attatttttc ggaatctctt aatctatatg
     2581 gatttcgtgc gacggccaat agtcatttag aatttcttag acagcaagtg caacaagata
     2641 ttctgctttt tattatttcc aattctggtg atacagttcg attagccgaa ttagctaaaa
     2701 ttgcgaacga tcactttatc gatgtgatcg catttgttgg gcaaaaaaat tcaaaaatcg
     2761 gtacgctggc aacattaacc attagcacgg aaacctatat tccacaaaaa atatctgatt
     2821 accaaccaaa tcttttcttt ggcacgacac tgaatcaatt tgagcttttg ttgagcgcgg
     2881 cgctgaaggc tatttataaa tagaagaacg ctgtggccac tgtttcttca aaatctagta
     2941 taaagaatct tctttagaat gtaagtattt tctgtgtgtt ctgaacatgt ttaggaattt
     3001 ccaaacttgt ttagaaaact ttctcattcc ctccgctgta gaattgaact cacataaaaa
     3061 acatctaaac caaaatatct acaagcttgg aaaggataat aggttgttat taaaaataat
     3121 ttttattcct gaataattca tagctttaat tctttaacaa atcatattct tataaattag
     3181 acaagagaaa ataaatattc catatctact ttattggatc cataacatga ctaaattctg
     3241 aatacttttt aaagcttatc atttggtaaa actgaatata agttaattat atagactgaa
     3301 aattttgaat tttcagaata gaataactta aaagtactga caaaattttt taaaggggca
     3361 atattaatgt tctgagataa tcaaatctta ccgaaaatat caaaagccta atgtgacaca
     3421 atcggattct gtcacattca tcaaaatcgc tccatcctta gaggctctaa gagaaaatca
     3481 cttttctttt aggtcctttt ttctatagtt tgtttaaatg tgacttatta tcagttataa
     3541 ttatattaag taacatttga gaggtggcta aaaatgaaga gcgtggaacc gattcgcgac
     3601 aaaaaaaaga tcgatgccat gaaggcaatt ttggcttccg gaaaatatgg gcaacggaat
     3661 ttggtgcttt tttcagtcgg gatcaataca gcctatcgaa tctccgattt aagacagtta
     3721 aaattaagtg atgtattaga aatttctcgt ggacgggtga ttgtcaagga acgtctggcc
     3781 atgaaggaac aaaaaacggc caaacacaat tcggtcttca tttcgaataa attacgaaaa
     3841 gtgattctcg actatgttca atctgaattt cctgagcagc tacaagtaca tgacttcagc
     3901 aattatttgt ttccaagtcg aaaaggagca gatacacctt tgaccagaca aagtctttgg
     3961 cgaatcattc atgaagcagg aacggcggtt ggcttgaaag aaattggtcc ccattcgatg
     4021 cgcaagactt tcggctattt tttgtataag caagggacca aaacagaaat cattcagtcg
     4081 ctgctcaacc attcttcaca acgggagacc ttacgctata ttgggatcac ccaggaagac
     4141 aaagataccg ccgtaaagag tttggatctg tgacagaata acgccttata taatagaagg
     4201 aaaaattcaa gaggtgagaa gatgaacgaa gaaaagcgtc ctaactttcc tgataaatac
     4261 caattatctc gcaaagagtc tgtgtattta ttgaagaaaa atattgtaga actggtgtat
     4321 aatgcgggaa agtttgaagg actgaataca acgctattac aaacagaaga gattattaag
     4381 tataatcggg ccaataacgt ggcggtagat gatgtcctga ccgtcgttaa cttaaaaaga
     4441 ggctttgaaa tgcttttaaa cgacgtacag gagcccctag tagagacaag taagcggatt
     4501 aatcggatag ttgcagcgga agatgccctt tttcctggtg aaataagaac aggaggagta
     4561 gaagtatcga ctattcaagg aagatatggg ccaccaatgt taactgaagg cgaggtgaaa
     4621 aaacagtatg acgagataat aaaccaaaat acatcagaga cggagaaagc gttacggtta
     4681 tttttattta tatcaaaaaa acaaatattt tgggatggta acaaaagaac tgctcttatt
     4741 accgcaaatt cagttatgtt caatcagggt gcaggattga tctcaattcc ggaaaaaatt
     4801 ttttcaaagt ttaatgagct tttatcagat ttctacaata gcaataatca catgtattat
     4861 gaaaaaataa aaaaatatat gtatttaaat tgtatcttcg gtatagattt tattggcaaa
     4921 tagaatgtga ggagtgataa ataattgaaa atgggaagta gtaaactaga agatgctgaa
     4981 aatagtgaac cagaagaagt agaagaaaaa acaatagacg ttgaatttga gcatctaaca
     5041 cctaatgata gtatggatct aactgcatat aaagaagcat tggactatgg aattaaaaat
     5101 agaatgacaa atattgcttt atcgggtccc tatggtgcag gaaagagctc agtattagaa
     5161 gcatataaga aaaatcatac tgataaaaaa tttatgcaca tctctttgtc tcattttgaa
     5221 ggatcgaagg aaattgatac taaaatttta gaaggaaaga taattaatca gctactacac
     5281 caaatagaat tcaaaaaaat accacgtact atttttaaag caaaagaaaa tcaatctaaa
     5341 tttaaaatga tatttgcaac tagtattgtt tttttattta ttactagtgg tatcataact
     5401 tttcaatttt caaaatggaa gaaatattat actagctttt tttcatccga gaaatttgct
     5461 tcatggttta caggcaacac ctttcactta atttcattgc ttgtttttac aagtatactc
     5521 gtatttttaa tttataattt tattaattta caagtaaatc agcaagttat gaaaaaaatt
     5581 attcttaaag gaaatgaaat tgaaattttt aaagatagtc atgaatctta ttttgacaag
     5641 tttatgaacg atgtaatata tttatttatt aattctggga aggatataat agtttttgaa
     5701 gatctggatc gatttgacaa tgctactatt tatgaaaaat tacatgaaat caatacatta
     5761 gttaatcgta gattagtttt gaaaaataag aagaaattga cattcatata cttaataaag
     5821 gatgatacat ttaagtctaa agaccgaact aaattttttg acttattaat tcccgtagtt
     5881 cctgttgtcg attcttcaaa ttcattcgat caattattat caatttttga aagaaataaa
     5941 gtgaaaaaag attttaatat aaatattatc cgaaaaattt ctttctatat agatgatatg
     6001 cggcttctaa aaaatataaa taatgaatac atcatttata aaagtaggtt aagaagtata
     6061 agtctggatt gcaataagct aattgcaatg attacttata aaaatctatt tcctgaagat
     6121 ttctccctct ttcagtcggg tgtaggatat attaatagtg ttattaaaag taaagaaaga
     6181 atattaagca aagaaattca aaagttatct gatgaaattg aattgcttaa cagttctatc
     6241 aatattgcta aaaaagaaca tttaaatgat atagatgaat tagatgcttt gtatttaaaa
     6301 ttggataatg atggatattt tagtgtagag gataaaaaag aagatgaatt cactacacgt
     6361 aaagacttca ttagagctat taaagataat aattttaaca ttatcaaata tacccctagg
     6421 tctggtagct ataggttgga atggcatcgc tcagaaatca atatttctgg caaatttaag
     6481 gaattaacga ataacgatga gtatagacta aggctagagg ctataaataa taaaagaata
     6541 atagatatga atcaaaacaa aattattaat ttagaaattg aaaaaaagaa tagaatgaat
     6601 tcatcgttat cagagatact aagcaatata aataataatt tttttataga cacaaattat
     6661 tttagcgaag aatttcatta tctatttaag agtcaatatt ttcctcttat tgttttcttg
     6721 ctcagagaag gattaatcga tgaaaattat ggagattata taacctactt ctatgaaaat
     6781 agcctaaaaa aagatgataa agaatttttg aggagcgcat atgacagaaa tcctaaggag
     6841 cttaattata aattacaaaa ctcaaatcaa attgttacaa atttgacacc tggtgacatt
     6901 actacttatg atataaaaaa tatagattta gcagcgtatt tagtttcaat ttatcctgag
     6961 aataatttgt atttaaagag tgttatagat gtgatgaaga gttgtgaaga taactcatat
     7021 atattggggt tgtttgaaaa gattaaaaat agtggggatg ttgaaaaatt tacaaatatt
     7081 tccaatgatt tttggcctac tatttttgca gatatcattt caaaatctga taagaacgat
     7141 gatattttgg aatttttgta tgtaatcagt tcatttgcta aaatacaatt tcttaaagta
     7201 aataatgagg ataatttact cacaaattat atatctgaaa ggaggttcat tcacccttta
     7261 attttatcaa aagagcaaca ggaggttttg ttagaaaagt tcaaaaaaat tggaataaag
     7321 tttcatgatt taaaaaaatc caatgcggat atagattctt taaaagcagt aattaattca
     7381 agaatgatag atatttcaga gtcgaatctt gaacaaattt tacaaatata tgatataaag
     7441 tattcgagag atgaatttaa atatagtaat attactttgt tctatgaaaa caatccagat
     7501 aatatttacg aataccttgc aaaagaaaag ataaacgagt atattcgagt atacttaaaa
     7561 tttggtatgg agactttaaa agaaaattcc aatgtatttg tagaaattct taattcggaa
     7621 aaattaaata aagaattagg atttgaatta attaaaaaaa ctaatctagc caatcaaatt
     7681 gaagatttaa aatatgtcat taatactgat tattggaata gtttattaat agctgagcac
     7741 ataaaaatag atgaacgaaa tattgtttcc tactacaaag aagcagataa tgatttttca
     7801 gaacaacttg taacagcaat caacaaaact actgtcaaaa ttacattttc aaaagataat
     7861 ttgtctgata aaactagaga agaattgtgg gaaactattg tccataataa tcaattggat
     7921 aataggcaat atatatcaat gcttaaaagt ttaggatttt attggagaaa tggctttaaa
     7981 ctaagtgtat cctctctaaa aattaaacaa ttaatctatg caaaaattat tcgtaatact
     8041 aaaaagaatt taaatgaaat tttgaataat cataaggtta acctagttga ttttgttcaa
     8101 gtagatatcg ataatttttg taatatattt atagacgaag atatatacca attcagtgta
     8161 attaaagact tgcttatgga aaaagaactt gtagattcaa aaaagaaaag aatagtagat
     8221 atttctaaac aagatatctc aatccaaaac ttgaatgtga gttatcgaat tcagaaatat
     8281 attttagaaa ataagtttga ggataatgat ttctcatata tcatagaaga atattctaag
     8341 tttaataacc tagtcaaatc gactatctat ataaaagcta tgtcaaacat agaaagaata
     8401 gttcaagaga aagtttcaat aaacattgaa ttgctgctag aaatgttatc tgatgaacag
     8461 atcagtgaaa gaaaaatttt attctcatat tatattcaac ttttagatga taaagatgta
     8521 attaagtatg ttagaagttt gaattttccg gaagaattta ttttagttct aaaaaaacga
     8581 cgtcctaaat ttgaaaattc cagtatcaat aaacgaattc tggaagatta cagacgaaga
     8641 gattggataa ccaaaatata tgatagagga aactatataa aagtacaagg aagaatggtt
     8701 ttaaagtagg cctataaacg aaaccagatc tatttaattg agatctggtt tttgaaaaaa
     8761 atttgataag ttattgagag aattatattc ttaaaattaa taaatattta atatatcatt
     8821 aagtttacat aagatataat tttgctatcc aaggccatct agttgaactg attatctgta
     8881 ttatattata tgaaaattag tttataattc ctagatctaa tgtatgaagt ataaattttg
     8941 tatctacata atagtttaaa agaaaccggt ataaattatt tagtattgct aatttgattg
     9001 agtatttgtt tcagctcgca aaatttattt aaaaaattga caccattaat ttcaaagttt
     9061 tataaaccag attaaattct aatagataaa tctgcctata ttttttgaca gtagaagttt
     9121 ttgtagctta ttcaaaagcc gatagatcga accaggtctt aagaaagctc gtaaatcttc
     9181 acagttttca aaacgttaat ctttttatcg aagattcgag acactctctt tttggagagt
     9241 gtttttttcg tatcaaatgg taagtttgag taatttttca ttgttattca gttcttttta
     9301 caatcaaata atttgattaa tacctctagc atcgatgacc atgatcgaag ttgctcatgt
     9361 gttgtttata gcgcattgat atcttgttga gtaatggttt taatcggttg attcttcttt
     9421 gtcaggaatt ctttattttc attttccgat gtcataaggg taccttcttc tatatttata
     9481 aacttcatgt ctatacgaac aacaatctaa aattattgta ctataccatg tttatataga
     9541 caacacgagg aggaaatata aatgaacgaa aatgaaggtg atagaaaagt ggaagtgcta
     9601 attaatgatt tattagcaga acgtcagatg tctttaagag aactcgcaag gttgtcggga
     9661 attgagccat caaatttaag caatttggca aatggaaaaa gacaaaaaat ttatttagaa
     9721 cacattgaaa ggattgcaga tgcgttggag attgatgata tcacgaggat attaaagttg
     9781 agtaggaatg tctaatttcc ctgactcata gcgggagtaa gtggtttggc tgatattcag
     9841 tagtttagca agttgttctt gattcaagtc tttgtcttcg cgtaagttgc gaattctatg
     9901 atacacaaaa tcacctcata tgataatatt aatacggtca aagagattac agcgtaataa
     9961 caaattgtac acacccgatt acattatcca tcaaaaagca taagaaaaca tcaaaaaacc
    10021 ataaatcaaa tctgttatta aatcatttcc ctcagatttg gtcttgcttt ccaaaacggg
    10081 tttctataca atagagggga atagaaaccg aataaaaaaa ggcaccccgt atcagagttg
    10141 gcgctccaat acgaggcctt gaaaagtcac gcgaactgac taatcaagtt gccatagtca
    10201 ataccctaag gtatcgacct gtttattgta cctaatattc tgttcttttt ctagtaggaa
    10261 aagcagattt tctgggattt tatgcaggat accttatgct tatttcgcta tgtcaaaagg
    10321 attggacagt cgtccaatcc tttttttgtg tttcagatgg ggaagttaaa ataacagagg
    10381 attgacgcat tccaattcta aaacagagtg tcggcaagcc tcggtttcta ttcgtctata
    10441 agaggttctg tagtttttaa ggtggatgta cagaaaaaat atgcttggta ttagatgcac
    10501 acggatagaa atttctgact tggttttatg atagcgctta attcattttg gattagatga
    10561 tatcactttg ccaagaaata gaaataacga gaagtcagtt gattgtcaaa cgaagcacga
    10621 aaaacacatc aggaggaagc tatgttccaa gaaaaaatat tcaatccagc aatactacat
    10681 gaaaaccgct tgtcacctcg aaccagtttt tttagttatg aaacggtcgc agcagctatg
    10741 caaggagatc cacaggcatc ttttgggtat cagtcattaa atggtctgtg ggatttttat
    10801 ttgggtgagg cagaaaatca agttgtgtat gaacaaaaaa tcgaggtacc tgctcattgg
    10861 cagtttcagg ggcatggaaa accaatctat acgaatgtcc agtatccttt tccaattgat
    10921 ccgccatttg ttgcgatgga aaacccaacc gccttttatc gaaaagcatt tattgtggag
    10981 gacattaatc ttgagcatgt gcttcacttt gaaggcgtgg aatcggtgtt tgaggtgcat
    11041 gtcaatgatc aatatgtcgg ctacagcacc ggcagtcgct tggtctctga gtttgatatc
    11101 acaccatatc ttcaaaaggg caaaaatgtg cttagtgtaa aggtgaagca atggagtgcg
    11161 atgagctatg tcgaggatca agatatgtgg tggcttggcg gaatttttag agaagtctac
    11221 ttgttttccc gtaagccgca gtcgattcaa aatgtaacga gccaagcaga tttatgcaat
    11281 caatacaaag atggcttctt ggcagtttcg cttttctttc aaccaacaac gacagaggaa
    11341 ccagaagaac gaacgataga aatcgaattg ctggatcagg aaaatgtaac cgttttaaag
    11401 aaagctgaga aaagactagg aggagcaaaa gcgaattgga cgatttccgc aaccattgaa
    11461 gagataattc cttggaacgc agaagaaccg tatctatacc atctgttggt gaaacagtat
    11521 caaggcgagg aactgttgga ggtcattccg ctccaagtcg gctttagaca gatttccata
    11581 accgacggta ttttccaaat caatggaaag cgggtagtgc tgaagggcgt caatcgccat
    11641 gaatggcatt gtgagtctgg cagaagtctg acagttgaga cgatggaaca agatgtgcag
    11701 atgatgaaag catttaatat caatgccgtt cggtgttctc attacccagc agacacaaag
    11761 ttttatcact tgtgtgatcg ttatgggctg tacgtcattg acgaagcaga cattgaaacc
    11821 catgggatgg atatcaccca aaggatcaat gaactaagta gtagtgagga atggttgccg
    11881 gcgtatttag accgagcaaa aagaatggtc gaaaaaaata gaaaccatcc ttcgatcatt
    11941 ttctggtcgt taggaaatga gtctggctat ggggaaaacc atttgaagat ggcgcagctg
    12001 atcaagaaat tagatcccag tcgtctggtt cattatgaag gagaagcacg gcacttgaaa
    12061 gccttgggag aatggcaagc gtgccaagcg tctgatgtca tttcgactat gtatacttct
    12121 gttggcgaaa tggcagaaca aggggaacgt tcactaacac aaccgcatat tttatgtgaa
    12181 tatggtcatg cgatgggcaa tggccctggt ggattgagcg aatatctgga attgttttat
    12241 cgctatcccc ggttacaagg cggctttatt tgggagtgga tcgatcatgg tatcaagagc
    12301 attgacaacg tttctggtga gacgtactac aagtatggtg gtgattttgc agaggaaatc
    12361 catgatggca actttgtgat tgatggattg ctatttccag atcgtacgcc atcgccagca
    12421 ttgttcgagt ataagaaagt gattcagcct gtcaaaatca ctttccagct ctcagaacag
    12481 gcctgtcgca tcaccaaccg cttcaatttt cgaaatttag cgtctttaca ggcagtcctg
    12541 caaataaaag aagacgatca ggtgattttc ttacaagagc taccaagaat tgaccttcca
    12601 cctcaagaag aaacaaccat ttacctgcca gaagaagcta cgctgagctt agcgggaaga
    12661 gcacaggcag tggtcacgct gtttttcttt gaagaaagtg cgccagattt ttctttagaa
    12721 cacgcagttg cttgggaaca agccgtcatt gatcaaccag taaaaagcgt acaaataccc
    12781 aaagcgaatg cctttcactg ttcagaaacg gccgatgaac tgaggattac tttggctgag
    12841 cagacctata tttttcataa gaaaacaggt accttgagcc agtggctcat cgaagggcaa
    12901 gccttgctta ctgaaccgct tgaaatgaac ttttggcggg caccgactga caatgacttg
    12961 ttaggcagtg aagagtttgg ttcggcaagc atcgccaagg aatgggaagc gtttcgcatt
    13021 cgccacttaa aggagcgtgt gctcgctttt acctatgaac agcgccttga tgaaataaaa
    13081 gtcgagattc gccgcaacag catgcctatt tccctcgact ggggaattga attgtccttt
    13141 acgtatcgtc tttcattggc aggcgacatc cgtgtagaag tccaaggaac cccgtttggc
    13201 aacggaccga aaaccctacc gcgaatcggt gtgcagctga tgattgataa acaacatcag
    13261 aatgttcagt ggctaggact tgggccgcat gaaacctatc ctgatagttg ccaaagcggg
    13321 tttttcgatt ggtgggaaag aaccgttcca gagatgcaca cgccgtatgt gcggccgcaa
    13381 gaaaatggca accacatgaa gaccaaattc ttccgtttgt ctaagccttc tggaggtgga
    13441 ctgactattt ccagcaaatc gctgaatttt agtgtgcatc actacactca gaacaatttg
    13501 gaacaagcga ctcataccaa tcaattagaa accagcgacg ccgttgccgt caacattgac
    13561 tatggggtgc aaggattagg aagtgccagt tgcggtcccg gtattttacc gaagtattta
    13621 ttgcaaaatc aggcatttgc ctttgatttt cagatgaact atcaaaaaga agtggaagga
    13681 gcatgacaat gaaaaaaata gctttatgta tggcaatgat tggactagga ggcttagctg
    13741 cttgtgggaa tggcagcgat acgccagagc gtgaaggggg cgaacaaaac ggcgcagagg
    13801 aaatcaccat ctgggcgtgg gacccaaaat tcaatattgc agcctttgag ttggcggaat
    13861 cgtatcatca agaagaaaat cctgatttca aaatcaatgt cactgagatt tcccaacaag
    13921 acagcgtaca aaaaatgaat accatcttta gttcaggcag tacccaagga ttgcccaata
    13981 tcgtcttgat tgaagatttt cgcgcccgta attttttaga agtgtttcca gagtatttcg
    14041 ttccattgac tgagtatata aatgttgacg attttatgga ttacaaaatc gaggtgtctt
    14101 caatcgatgg cgagcagtat gggatgccgt ttgattcagg ggtagcgggc gtttacttgc
    14161 gaaaatcgat cattgaagaa gcgggcttgg aactgtcaga ttttaccaat atcacttggc
    14221 aaacctttaa tgaattgggg atccgtgtga aagaagcaac tggcaaacca ttgttgtcca
    14281 tcgatatgaa tgatctagga catttgcgaa tgatgatgca ttccagtggt tattggtata
    14341 ccgcagagga tggtgaaacg ccttttattg acggcaatcc ttctttgaaa caagggcttg
    14401 aagatttgaa aatgctgctt gattcaggag taatgacaac gcataatggc tgggaccaaa
    14461 tgctagcagg ttttaacagc ggcgaagtag tgggcgtttt acaaggcaac tggatcacgc
    14521 catcggtgaa agcagaagaa agtcaaagtg gcgattgggc agtcgttcca gtgcccaagc
    14581 aagaatcgat cgaaggttca gtcaatgcga ccaatctcgg aggctcgtca atttatgtgt
    14641 tagatatcga tgggaaagaa gaagccctga gctatattgc tgacgtattc ggcaaaaata
    14701 tcgcctttta cgaagagctg attcaaaccg tcggagcaat cggttcttac gttcccgctt
    14761 cacaaggaga tgcgtatcat gcaccagatc ctttctttga tgatcaggct atctatcaag
    14821 atttcatgaa ctgggcaaat gaaatccctt cggtaaactt tgggaaaaac acttatgcgt
    14881 tggaagacat catgaatcaa ggctttttgg actatgtcaa tggagcagat ttggatgagg
    14941 tcttaaaaaa cacgcaaatg attgctgaca atcaattgaa ttaacacgga ctgaggggca
    15001 tgtacgatga aaaataaatt attttataaa aaaggagtga tgacggggtg gctatttatt
    15061 gctatcccga cactcttaat cttctttttc tctttttatc caatgatcca agcgtttcta
    15121 ttatccttgc aatcaggaag cggcaccggg ctgaccttta gcggactagc gaattatcgg
    15181 cggctatttt cggatcaaac ctttttgaca gccacgaaaa ataccttgat ctttttgggc
    15241 tttcaagtgc ctgtcatggt cttgttggcc atgttctttt ccgtccagct caatcagaaa
    15301 accctaaagc tgaaaggcct gtttcggacg atcatttttt tgccagcagt aacttcatta
    15361 gtcgcatact ccatggtctt taaatcactg tttgcaacaa atggcgtggt gaacgccctt
    15421 ttagtgagac tgtcgattgt ctctgagcca atcgcctttt tctccagtcc cttttgggca
    15481 aaagtcatta tcattttagc catcacttgg cgctggaccg gctacaatat gattttcttt
    15541 ttatcggctt tgcaaaatat cgacccagca atttatgaag ctgctagaat cgatggcgct
    15601 tctccaacga aacaattttt taaaatcaca gtccctttgc tgaagccagt cattttgttt
    15661 tcagccgtga cctcaacgat tggcacacta cagttatttg atgaaccgat gaatttgacc
    15721 caagggggac caggaaacgc cacatcgacg atttcccaat atatttataa tctttctttt
    15781 cgctatacac cagattttgg ctacgcctct gcagtttctt atgccattgt cgtcatggtc
    15841 atcttctttt ccatgatcca atttaagatc ggaggagagg acaaatgaaa aaagtaggca
    15901 catatgtgtt tttaggtgtt tttaccattc tgtttgcctt tcccttttat ttcatgttag
    15961 tgagtatcac caaccctacc gtaaatatca tgcaaggcaa actattgcca agtacccatt
    16021 tgctagaaaa ctggcgtaac ctagtcgcaa cctatgactt gaaacaagta ttctttaata
    16081 gtgcaagaat tgcgttgtta cagacgattt tatctgtgac gatcgcttcg ttggccggct
    16141 acggatttga gatctatcaa agccgattaa aaaatatgct ttttaatatg ttgctcttgt
    16201 cgatgatgtt gccttttgcc gccaccatga ttccgctgtt tcggatgttc ggtagctttt
    16261 caacgacgat tccttttatt ggactcgata cagtagcttc agtcattttg ccaacattat
    16321 cgaccgcatt tctgatcttc tttttcagac aaaacacaaa aatgttttcc aagtcactgc
    16381 ttgaagcagg gcgaatggat ggcctgtcgg agctaggctt gtttttccga atatacatgc
    16441 caaccatgaa aacaacttat gcggccgctg ccatcattac cttcatcaat agttggaaca
    16501 gctacctgtg gccgttgatc acccttcaat caccagaaaa gcaaaccttt cccctgatga
    16561 tttctgccat cggcgccgcc aataatccag attatggctt aatgatgtta gcaatcgtga
    16621 tcgcaactct gccaacaatg gtggtcttct tctcgctgca aaagtacttt gtggctggga
    16681 tgttgggatc ggtgaagtag ggagaagcta tatgcgagaa acgattagct catcgttaga
    16741 aaatttgatt ccttgaaata atttctgtgt atcgatttgg agtaaattca caatttttgt
    16801 gagtttactc tttttctcta atttctttca gttggcggat gaaaagtgaa tactcctaat
    16861 tcctgcattt ttttcaatcc tgattggcga tcttagtttt ccgagattcc cctaaaaggc
    16921 tcatctaaca gctcctaacc ttatatacat ttacatatct aactcttatc ttttcgcttt
    16981 tagttagtta aaaagcaaaa aataaatgtg aatccgagct atttttaaat aattaaccag
    17041 ataatcttcc gattggaacg gttaaatagt gaatgattgg tagaaaagag attagaggat
    17101 gcgagttgtg ataagtaaac tcataaaaga agaatgctag aaaagtagag aaaaatagga
    17161 ttaatgaaat aatcgagatc cttatttttt aacaattttc gaaaaccata aatgaattga
    17221 aggaagctta gtgtgggtga aagaataatt acaaaaaaat attgacttaa aaaaatggct
    17281 atggtaatct ataaacggaa atagtgggaa cgtttccaaa atgcatcttc gttaaatttt
    17341 tattttttta aacgaaaatg gaaacgtttc taaaatgtat tgggaggaga tcgaattgga
    17401 aaagacattg ggaccaggtg ttgagataag ggcggtacaa cgggataaaa aaaatatctc
    17461 gaagtgggaa aaattaaaga aacagttgcg gcgtgataag tggatgtact tgttgctctt
    17521 accaggtatc atcatgacgt ttatctttaa gtatgtgcca atgtacggtg ctgtgattgc
    17581 atttaaagac tataatccta tgctaggaat tttggaaagt ccttgggtag gactagatca
    17641 ctttagggaa tttatcagtt caccaaattt tacgcttttg ctgagcaaca cattaaaaat
    17701 tagcatttat ggtttattgt taggtttttt tccaccaatt attttagcac tgagcttgaa
    17761 tcaaattatc aataaaaaaa taaaacagaa aatccagttg attctatatg cgccgaattt
    17821 tatctcagta gttattatcg tggggatgct gtttttgttc ctatccgcaa atggacctat
    17881 taatgggtta gtagaaaagg ttacggggca accaattatg ttcatgtcta atccagctta
    17941 tttcagaacg atttatattt tatcgggaat ttggcaagga atcggctggt catccattct
    18001 ttacaccgcg acgctggcgg gagtcagtac cgatttatat gaggcagcaa atatcgatgg
    18061 ggcaaatatt ttacaaaaga tttggcatat cgatttgcct gcaatgaggc ctgtgatggt
    18121 gatcagcttc atcttggccg ccggcggtat tatgaatgta ggatatgaaa aagcctactt
    18181 gatgcaaact tcaatgaaca tccctgcctc agaaatcatt tccacttatg tatacaaagt
    18241 cggcttgcaa tcaggtgatt atgcctactc tgcagctgtt ggtctgttca atatgattat
    18301 taatgtcgtt ttattactga ttgtcaacaa tataaccaag cggctaaacg atggtcaagg
    18361 cttgtaatga aaggagaaaa ttatgaatag tgctttatca aaaatcgacc gtcaaatctt
    18421 atggttgaat cgagtgattg taactttttt agtcgtcatc accattgttc cgctgattta
    18481 tgtactggta gcttctttta tggacccagc tactttgatc aataaaggta taagcttcaa
    18541 tccgaaagat tggacaacac aaggatatca acgggtattt gcagatgatt ccattttacg
    18601 cggttttgta aattcgttgt tttactcttt tagcttcagc ttattgactg tatttattac
    18661 gatgatcact gcgtatcctt tgtctcgacc taacttggcg gggaaaaaga ttattatgac
    18721 tttttttgtc attacaatgt tcgtgggtgg cggcttgata cctacctatt tattggtaaa
    18781 gaacttgggc atgttagata gtgtatgggc gattatcgta ccaggtgcta taaatgtgtg
    18841 gaatattatc ttagcccgta cctattttag tgctttgcca aaagaacttt ctgaagctgc
    18901 tactatcgat ggcgccaatg acttgcaaat actgttcaaa gtcatgcttc cattagctaa
    18961 accaattatg ttcgtattgt ttttatatgc ctttgttggg caatggaact catattttga
    19021 tgccatgatt tacatcaaag atcccgactt acagccgttg caactcgtat taagaaatat
    19081 tttgatccaa aaccagccct cacaagacat ggtgggagcc aatactgcca tggcagaaat
    19141 gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc atttctagtc tgccattaat
    19201 cataatgtat cctttcttcc aaaaatattt tgataaaggt gttttagttg gttctgttaa
    19261 agggtaagaa ttgttcacta tttgtatgaa taaaaattat aaaggagatt ttaagatgaa
    19321 agcaaaatgg atgctgccaa tcgtttttac ttccgtacta attttggggg cttgtggagg
    19381 ctcaaaagaa aagagtacat caagccctga ttatgaacta gaaaatgtct cttttccatt
    19441 gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc cctttggctc ccaatgatcc
    19501 taatgacaaa ttgattttcc aacggttaga agaagagacc aatctccata ttgattggac
    19561 aaactacagt agtgattttg ccgaaaaaag aaatttggat atcgctagtg gtgatttacc
    19621 agatgccatt ttcaacgccg ctgcaggtga ctatgattta ttaaactggg cggaaagtgg
    19681 cgttattgta ccgattgaag atttgatcga agattatatg ccaaatctga agaaaatttt
    19741 tgatgaaaat cccgaatacc gacaaatgtc tactgcacct gatggtcaca tttactctat
    19801 gccttggatt gaagaattag gcgaaggaaa agagtcgatc catacagtta atgggatggc
    19861 ttggattaac aaggagtggt tggataattt gggtcttgaa atgccgcaaa ccaccgatca
    19921 gttgatgact gtgttggagg ctttcaagac gcaagatcca aatggtaatg gtgaagcaga
    19981 tgaaattcct tttagcttta ttaacaatgg aggaaatgaa gacttaaaat ttttaatggg
    20041 ggcattcggt ctaggtgata atgatgacca cttagtcgtt gataacgatg gccaggtaca
    20101 atttactgct aacaaagaag ggtataaaga agccatcaaa tatttcaatg aaatgaataa
    20161 taaaggtctt attgataaag aggctttcga acaagattgg aatacgtatg ttgccaaagg
    20221 gaaagaacat aagtatggcg tgtactttac ttgggataaa gcaaatatca cgggtgcaga
    20281 tgatagttat gacgtcttgc cagtcttagc gggaccagat ggaacgaaac acgttactcg
    20341 tacgaatggc atgggtttct caagagatcg tttcgtaatt actagtgcga ataaaaactt
    20401 ggaacttaca gctaggtgga tagataaaat gtatgaaccg ttacagtctg tccaaaataa
    20461 ctggggaacc tacggtgatg aagcacaggc caatattttt gaattaaagg atggtatgtt
    20521 aaaacatttg cctttagagg ggacagcacc gggggaacta agacaaaaaa ccgaagctgg
    20581 aggaccatta gcagttcttg attcttatta cggcacagtc actactatgc cagatgatgc
    20641 gaagtggcgt ttagatttga tgcatgacac gtatttgcca tatattacag aagacaatat
    20701 ctatccacgt gtctttttag aaaaagaaga aacagaccga ttagcaaaaa ttgaaacaga
    20761 tatgtgggat tacatctatc gcaaaagagc agagtggatc accggcggta aaatcgatga
    20821 agaatgggaa gaataccttg ctgaattgga acgtgtaggt ttggaagaat ggattcaaat
    20881 aaaacaaact ggctatgata attttttgaa gggagcacaa taagtgttag tagaagaaac
    20941 ggcgattttg gataaagcaa atcgatttat tgaaatgatg cgaggaaaag ttaaccctca
    21001 gtatcgacaa aactatcatt tttccgcacc catcgggtgg ataaatgacc ccaatgggtt
    21061 tatatattac aaagggcatt accatctctt ctatcaatac tatccttacg atagtcgatg
    21121 ggggcccatg cattgggggc atgcgcgcag tagtgatttg gtccattggg aagatctacc
    21181 agtagcgtta gccccagatc aaccttatga cagcggcggg tgtttctcag gaagtgcaat
    21241 tgaaagagac ggaaaactat atttatttta tacaggctgt caagaaattg atggtattag
    21301 ccatcagcat caatgtttgg cggtctctga agatggtatt cattttagca aatacagtgg
    21361 taatcctgtg gtagcagaaa aacagctagg tgagaatggg ctgatacaag aatttcgtga
    21421 tcctaaagta attgttcaca acgatgctta ctatatggta gtagctacta aatctacaca
    21481 gtctactggt agaatagtga tgtttcaatc gtcagatttg tatacttggc acttttactc
    21541 tgtgttgcta gaagggactt cagagcaagg aataatgtgg gaatgtcctg acttattcca
    21601 tttagacgga cgagatgtct taatcatgtc accgatacaa atcaagaaga acggactgga
    21661 atatcataat atcagttcta ccatggcttg tattggtgag gtagactggg aaacaggtca
    21721 gatgatagtt gataatttcc atgaaatgga ttttggaatg gatttttatg caccacaaac
    21781 aacaacagat cctaatggtg gacgggtgat gattgcttgg atgcagatgt gggatcgcac
    21841 attgcctaca catgagttag gccatctttg ggcaggctct atgagtttgc cccgagagtt
    21901 aagagtgaaa aaaaatcgat tggtacaaaa acccatctca actgtatata gtcatttagt
    21961 ttatcaacaa ggcagtgaaa atattgcagt gactgacaca tcagtcgtat ttcgaaacgc
    22021 gctgtctgat tcttcatatt tacatctagt cgcagatttg gagaaagcag aagtttttac
    22081 aattgaatta ctgcggtcta agacggattg tctcaaacta acttatgaca ctgctaaaca
    22141 ggtttttgaa ttggatcgtc agaaactagg aagagagctt acaggggcgg aagaagaccc
    22201 tctgaatgtc cgaaaagttg aaataccttt gattgataat cagctcgttc ttgagatttt
    22261 tagagatacc tcatcagttg aagtctttat taatggttta gaagttatga cggctacttt
    22321 ctatgagctg gaaaaagctc gagatatctt atttcattcg atcggagaaa gtcgtattca
    22381 agcttttgaa acagcgagtg tgaaataaat gtttgattgc ttaggcgatg aatcaattat
    22441 ttaataggtt ttgaaggata attttttaga aaagaacaga tagctagatt tcccatggct
    22501 ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat actaaacttg
    22561 tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca aaattagcag
    22621 gtgtcggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa gcttctacta
    22681 gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt tatgcccggg
    22741 ggttaaagat gaagcatacg aatacggttg ctttgattct acccactgta tggcatccat
    22801 tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac tacaagcttt
    22861 atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag atggtcagcc
    22921 aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa tacatttcga
    22981 gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt tatgtgaccg
    23041 ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga ggatgtcaga
    23101 atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt cgtcgaaaat
    23161 attttgagat cgagtgccgt caactgggga tttcctataa agcgttagat atgttagaac
    23221 ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc gatatcgatg
    23281 ggatttttac gatcaacgat ccgatggcat tagctgtcat tggtatgctt gaaaaaatag
    23341 ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag atgacagaag
    23401 gagaccctta tctcgtctca acaattgccc aacctacaaa gcaaatggcg gaagtggcag
    23461 tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc gtattgccga
    23521 ttgaatttgg tgaaggtgga acaacgaaac ctttagtgag aaacgatttt gcttagtgca
    23581 aatacgtttc tttttttgaa gataagtgaa tgaaaggtgt agataaaatg ttatatggtg
    23641 gaattgaagc tggtggaaca aagtttgtct gtgggattgg taatgaacaa ttacaagtga
    23701 tagaacggaa aagttttcct accacaacac cagaagaaac gatgaagcaa gtgtttgatt
    23761 tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt gggccaattg
    23821 atattcaaat cgaatcaaga acttacggat atatcaccaa caccccgaag ttagcttggc
    23881 aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc tattggacta
    23941 ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa ggtaaaaata
    24001 gtgttcttta ttacaccgtt ggtactggtg ttggtggtgg tgctgtagtc gatggaaaaa
    24061 tagtcacagg tttcagtcat cctgaaatgg gacatatgct cttacaacgt cataatgaag
    24121 atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta gcctcaggcc
    24181 ccgcgattga aaaacgcttt ggaagaaaag cccagaccat ttcatttgat catccttatt
    24241 gggcgattga ggcagattat cttgctcagt gtgtgtataa cacaacttta ctatatgcac
    24301 cggaagtaat aattttaggt ggaggtgtaa tgaaacagcc gcaactactc gagttgattc
    24361 gccaagcgtt taaagaaaaa atgggttcct atgtggatac ccctgctctt gaagaatact
    24421 tactaactcc aatgcttgct gacgatgcag gattgatcgg ctgcttagct ttagcaataa
    24481 agaagttgga acaatagcag catgttaaag aacgtgtgag agagttttta tagcagttag
    24541 gatagataaa aaggttaact aaaatgtgag ctagaaaaaa ggatggctaa taatagatgt
    24601 tcatatatat tttttgcgta atcgaatgtc gcaaataaga attttttctt tcgttctcgt
    24661 cttcagttat attgttgtct agctgcctca aaattacttc ttagtgcttg gtttttgttt
    24721 tatcacagat attcattata gaaagacacg atgtgaaaat ctattattct cggtagttta
    24781 ttgcttctag ttgggatgat tgcgtagaaa cttaacgtca gttaaagtgc gtcacgaata
    24841 gtagtaacga gaactaatga gacattagaa tgttataaga aacccgtcag aagagtctac
    24901 ctgacgggtt ttataattat tagaaggctt gctcctcctc acatcttcgc aatcttccgc
    24961 accgtgttga tagtccgaat cgtaatctgt ttgccgacgc tggtactggc tgtttttttc
    25021 caccaattgg ttttttgata gtctttgcgg atgtaggacc agaagacgac gtctttgtaa
    25081 gggaaaacct gttcaaggtt ggcatttgga gccccgagtt tttcagataa cacctcgtag
    25141 gtcagtgaag gcatcaaaaa gatcaggttg tcatagatgg ttttatcttc cgttcctgac
    25201 caatcaggta tatgggccaa gcgttcctgc agctctgatt ggcgaatgat ataaatagcg
    25261 atatcaaagg aaaagtcgcg tttgaccatt gttttaatca tttcggcaag ggcttgtgga
    25321 tcatctcctt cactggaaaa aatcacattg ccgctattca aatacgtgac cacatctcta
    25381 aaccccagtt cttcaaaacc ggctttcaat tctttcatag aaattttgtt ctttccaccg
    25441 acattgatgc ctcttaataa agcgatgtat cgtttcatgg ctagcccctc ctttaaaacg
    25501 cttcttttag ctgattatag aatggatgga gcggaagcac aatcctggct tctcagctac
    25561 ccaaaatacg tgagcgagaa gcgattaatg caagcttatt ggtggagtgg ccgtggcgta
    25621 cgtatttttg cactgctgat tagtaaaaac gaagaaacag cctatagatg gtaatacccc
    25681 tcacaaattt gctaaagcaa gcgaatttgt gaggggcact ttaagttgtt ttctctattt
    25741 ctggtttcat tgttaatcca ccgcaatcct cacagacact gtctgtacgg ggacatcata
    25801 aaagggatct tctcgtcggc ctaaaaagtc ttgggcttgc tgagggaaga gggcgtacat
    25861 cagcacgtct tcttcgcttt tggcatactc agcgatttct tccttgaagg ctggcagttg
    25921 cggtgccagt agatcagctg gtctgacggt gatcacttcg gcatctccga taattgtttc
    25981 tttgatttct tcgctgatgg gtgctggcga tacaactatg gcaatatgtt ttatgtgcaa
    26041 ctggtaacgc agtatcttgt cacaacagag tttgatgatg atacggtaca agccatcatg
    26101 gacgaagcac tgaaatatga gggctggcga tacgtttacg gtggagcttc cccgactact
    26161 tcttttgatt gtagcggact gacacaatgg acgtatggaa aagctggaat taacttacca
    26221 cgaaccgcac aacagcaata tgatgtgacc cagcatatcc cactatcgga agcacaagct
    26281 ggcgatttgg ttttctttca ttctacctat aacgctggct cttatattac tcatgttggg
    26341 atataccttg gcaataaccg tatgtttcat gcaggcgacc caatcggtta tgccgactta
    26401 acaagcccct actggcaaca gcatttagtg ggagcaggac gaatcaaaca atgagaaagg
    26461 aagatttaat gatgaaattt agaaaaaatc agaataaaga aaaacagata ccaaaggaaa
    26521 agaaacctcg tgtctataag gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac
    26581 ttttagggct tagtttcagc tttgcgatat tcaagcactt tacagctata gatactcata
    26641 ctattcacga aacaactatc atagaaaagg aatacgttga tactcatcat gtagaaaatt
    26701 ttgtagagaa ctttgcgaaa gtctactatt catgggagca atccgataag tccattgata
    26761 atcgaatgga aagtctaaaa ggctatctga cagatgaact tcaagctctc aatgttgata
    26821 cagtacgcaa agatattcct gtatcgtctt ctgtaagagg atttcagata tggacggtag
    26881 agccaactgg cgacaatgag tttaatgtaa cctacagtgt agaccagctc attacagagg
    26941 gagaaaatac aaagaccgtc cactctgctt atatagtgag tgtctatgta gatggttctg
    27001 gaaatatggt actggttaag aatccgacca ttaccaacat acctaagaaa tcaagttata
    27061 aaccaaaagc cattgaaagt gaggggacgg ttgattccat tacaaccaat gaaatcaatg
    27121 agtttttaac gacgttcttc aagctctatc ctacagcgac agccagtgaa ctttcctact
    27181 atgtgaatga cgggatatta aaaccaatcg gaaaagagta catctttcaa gaactggtaa
    27241 atcctattca caatcgtaag gataatcaag tcacggtatc gctgacagtg gagtatatcg
    27301 accagcagac caaagcaacg caggtatctc aatttgattt ggtacttgaa aagaacggga
    27361 gtaattggaa gattgtaaaa taacaaatat tggtacatga ttacagatac tttgtaatca
    27421 tgtactcttt ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
    27481 tttaagtgac tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
    27541 tcaatttgat agcaggaaca aataattaga tgtccttttt taggagggct tagttttttg
    27601 tacccagttt aagaatacct ttatcatgtg attctaaagt atccagagaa tatctgtatg
    27661 ctttgtatac ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
    27721 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
    27781 gttttagctc atgttgatgc
//
LOCUS       DACELF010000032        26342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.032, whole genome shotgun sequence.
ACCESSION   DACELF010000032 DACELF010000000
VERSION     DACELF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.032"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(88..1218)
                     /locus_tag="IW687_001290"
     CDS             complement(88..1218)
                     /locus_tag="IW687_001290"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0950562.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1867..3069)
                     /locus_tag="IW687_001291"
     CDS             complement(1867..3069)
                     /locus_tag="IW687_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0950563.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="IW687_001292"
     CDS             complement(3129..3332)
                     /locus_tag="IW687_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0950564.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="IW687_001293"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="IW687_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4869..5681
                     /locus_tag="IW687_001294"
     CDS             4869..5681
                     /locus_tag="IW687_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950565.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6705..6857)
                     /locus_tag="IW687_001295"
     CDS             complement(6705..6857)
                     /locus_tag="IW687_001295"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950566.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7048..7926)
                     /locus_tag="IW687_001296"
     CDS             complement(7048..7926)
                     /locus_tag="IW687_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0950567.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7965..9083)
                     /locus_tag="IW687_001297"
     CDS             complement(7965..9083)
                     /locus_tag="IW687_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0950568.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9080..11179)
                     /locus_tag="IW687_001298"
     CDS             complement(9080..11179)
                     /locus_tag="IW687_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0950569.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11230..12051)
                     /locus_tag="IW687_001299"
     CDS             complement(11230..12051)
                     /locus_tag="IW687_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0950570.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12048..12857)
                     /locus_tag="IW687_001300"
     CDS             complement(12048..12857)
                     /locus_tag="IW687_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0950571.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12878..13372)
                     /locus_tag="IW687_001301"
     CDS             complement(12878..13372)
                     /locus_tag="IW687_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0950572.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13384..13872)
                     /locus_tag="IW687_001302"
     CDS             complement(13384..13872)
                     /locus_tag="IW687_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0950573.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13949..16645)
                     /locus_tag="IW687_001303"
     CDS             complement(13949..16645)
                     /locus_tag="IW687_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0950574.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17073..18146)
                     /locus_tag="IW687_001304"
     CDS             complement(17073..18146)
                     /locus_tag="IW687_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950575.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18143..18970)
                     /locus_tag="IW687_001305"
     CDS             complement(18143..18970)
                     /locus_tag="IW687_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950576.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18971..19777)
                     /locus_tag="IW687_001306"
     CDS             complement(18971..19777)
                     /locus_tag="IW687_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950577.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19789..20874)
                     /locus_tag="IW687_001307"
     CDS             complement(19789..20874)
                     /locus_tag="IW687_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950578.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20891..21433)
                     /locus_tag="IW687_001308"
     CDS             complement(20891..21433)
                     /locus_tag="IW687_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950579.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21691..22680
                     /locus_tag="IW687_001309"
     CDS             21691..22680
                     /locus_tag="IW687_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0950580.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22677..23414
                     /locus_tag="IW687_001310"
     CDS             22677..23414
                     /locus_tag="IW687_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0950581.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23411..23962
                     /gene="coaC"
                     /locus_tag="IW687_001311"
     CDS             23411..23962
                     /gene="coaC"
                     /locus_tag="IW687_001311"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0950582.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23978..24610
                     /locus_tag="IW687_001312"
     CDS             23978..24610
                     /locus_tag="IW687_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0950583.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24695..25342
                     /locus_tag="IW687_001313"
     CDS             24695..25342
                     /locus_tag="IW687_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0950584.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25347..26330
                     /locus_tag="IW687_001314"
     CDS             25347..26330
                     /locus_tag="IW687_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0950585.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8052 a         5571 c         4466 g         8253 t
ORIGIN      
        1 ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga ctttttggga
       61 cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga cttgctgcaa
      121 taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt ctgttgaagc
      181 aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt gtgcttcttt
      241 tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag taactaatcc
      301 taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat ctcctgctcg
      361 gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact cttctgcaat
      421 gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc agatatgtgt
      481 tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct ctgtacaaat
      541 atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt tagtttgatc
      601 agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt tttcataaaa
      661 agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag atagatcagg
      721 ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc gtaaaatcat
      781 cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca ctacttcttt
      841 cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc tatctactac
      901 ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc cagtttctgg
      961 aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa atccacgccg
     1021 aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca ctaatttttt
     1081 tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt cttgaacttt
     1141 ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat ctactgtaaa
     1201 tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc tacgccttcc
     5821 tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga cctggagttt
     5881 gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca taatcagcgc
     5941 gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg gttagttaat
     6001 attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca acctttaaaa
     6061 atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat tctcaaattc
     6121 tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt tccatttttg
     6181 tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga aaaatttttg
     6241 tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc aaattatttt
     6301 tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc ctaataaaga
     6361 aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt cttatttttc
     6421 actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca atcatttcaa
     6481 ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt gcaagagaag
     6541 cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata ggaataaaat
     6601 atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa tcacaactct
     6661 ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca aaaaaattca
     6721 aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca ttggtaagca
     6781 actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa gtgtacgcca
     6841 ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa acgcagaagc
     6901 atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt tttatttgaa
     6961 ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa atacttttga
     7021 catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg cttttctggc
     7081 caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa taatgtagct
     7141 atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat cctgaacttt
     7201 tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat ctggcgaaaa
     7261 catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct gccaaaacga
     7321 atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg ctggtcctgc
     7381 tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat tgtcctttgg
     7441 atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca cgaaagtccc
     7501 attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa cacctgctaa
     7561 ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat ctgttgtcca
     7621 ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat tttgccaagc
     7681 aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa tatcaattgg
     7741 accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa aaaaatcaat
     7801 gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt ccaaaatcgt
     7861 taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa ttgccccata
     7921 tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg cggcaagcgt
     7981 tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata ccagtaagcg
     8041 acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc gatttgttgt
     8101 aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca acgataaaga
     8161 ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt tcccgtttca
     8221 aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa ggctgttgaa
     8281 tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca cgccccgcca
     8341 aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc atcaatgtag
     8401 aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc aaggtaagta
     8461 cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc ctgtaatttg
     8521 gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc caattcgtcc
     8581 accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg taaattctcg
     8641 atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa gccccctgta
     8701 ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc attacaaaag
     8761 aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata aattcttaag
     8821 attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt catccacata
     8881 tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc agaagtcagc
     8941 ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat cgcagcttta
     9001 cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt tttaaattgg
     9061 gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt tttccctttt
     9121 ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac aaatttgaaa
     9181 tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac ttcaaattct
     9241 tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt ttgaatttca
     9301 atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag ccgcatttgc
     9361 cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac ggccaccacc
     9421 accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt acgaaagctt
     9481 gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg gcgccggatt
     9541 ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa gaactagcag
     9601 aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga taaaaaggca
     9661 tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt ccatccacaa
     9721 tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag tgcttcactg
     9781 aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca aaaggaccat
     9841 cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt ttgtatcgct
     9901 tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata caggccttcc
     9961 aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct gataaccaaa
    10021 cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc tcaataattc
    10081 ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc ccagtatgtt
    10141 caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa tgattgatac
    10201 ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat agaaccgttt
    10261 tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac atatccttat
    10321 tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc tgtccctgtc
    10381 gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga tggacaactc
    10441 tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat gtttccttca
    10501 gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca tttgggtcca
    10561 caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta tttccttttt
    10621 gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc acatctttca
    10681 ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga acaagcatac
    10741 ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta aacgtcgaat
    10801 aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata cgatgatccg
    10861 attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac gaattggttt
    10921 gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa ttttctcgat
    10981 aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc ttttgattac
    11041 ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg tattcgtttg
    11101 aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg ggtgaataaa
    11161 ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc tagtagctgc
    11221 agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga ccattccaaa
    11281 aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca atgttaccaa
    11341 taacggtaca attcctttaa aaatactatc gaggatttct tggacattca ttagttcttc
    11401 gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg tcattgcacc
    11461 taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta acccgttcgc
    11521 atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc catagtatcg
    11581 catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca aaatatttcc
    11641 ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc cccagaacaa
    11701 tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt tgatactttc
    11761 cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa tccccatgac
    11821 aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca gcgccgcagc
    11881 tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat aggcgaaacc
    11941 accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc gccaaaacat
    12001 cttccgaacc aattttttct catctttaga aattccatca gctaatttca ttgaaagaaa
    12061 tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac tgcatgaaca
    12121 gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat cgttccaact
    12181 gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc aaagaaaaac
    12241 cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc aactgccggc
    12301 agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt atccaagacg
    12361 cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc ataaagcgca
    12421 tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc accagatttt
    12481 gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat catattcatt
    12541 aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc cacgtttcct
    12601 cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc cgccgtacta
    12661 gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc gacaatcaac
    12721 cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat cggacgactc
    12781 agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc caaagcgact
    12841 acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc aaaatagaga
    12901 tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga taaagttcaa
    12961 ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt gataaggttt
    13021 tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt aattcgattt
    13081 ccgttgggaa agctttcgca acttttaccg catctttgac attagggact aaaaccatca
    13141 agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca actgttttaa
    13201 taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga atatcgttgg
    13261 tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt gtccaagtca
    13321 tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc atttttcttc
    13381 ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa tggtcatctt
    13441 taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta attccttggt
    13501 tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag ttacagcaat
    13561 ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa ataaatcggt
    13621 aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca actcctgttc
    13681 aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct tccccgtcag
    13741 taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta ttagaatttt
    13801 cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc ctgtgccaac
    13861 ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct aacctaaaaa
    13921 aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct aataattcat
    13981 agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc attgattgaa
    14041 aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca taattcggtt
    14101 cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc gtatgcatat
    14161 agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca tatttagaaa
    14221 tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa cggtccgcaa
    14281 ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat tctgccggct
    14341 tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct aagatgtcat
    14401 aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg atgaccaatt
    14461 ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt tctctgtagt
    14521 ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca tctttcaatt
    14581 tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt tgagaaaagc
    14641 cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca atctgtttaa
    14701 atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc gtcaaattat
    14761 tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt tgatttaaag
    14821 agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc acgaaattcc
    14881 ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac gcttcaaaaa
    14941 cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta ctatctccgt
    15001 gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat aaagtcacga
    15061 caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa tgctccaaga
    15121 actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga tttatcccgg
    15181 ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt tctgcaacag
    15241 gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc tttctaaaca
    15301 tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg tgatgcacca
    15361 aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct tcatttatca
    15421 gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga tccgttttta
    15481 tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact tcggattcca
    15541 gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct tcttgtgttc
    15601 gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca atattcccac
    15661 taaagggatg agataacatt aaagacaagg cttctcttga aacaataata tctttttgta
    15721 aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct cgaatggggc
    15781 gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag gtgtctaata
    15841 aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca gaatgccaat
    15901 ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag agcttttctt
    15961 gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg ttggccgttg
    16021 ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg tatccaaaca
    16081 gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag atagcaaatg
    16141 gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt gtggctaaaa
    16201 aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta ggcggatatt
    16261 tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga ccaactacag
    16321 aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca tctaaaggct
    16381 tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact aatctcgtca
    16441 aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc tcggcagtga
    16501 cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct ttagcgctct
    16561 ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc ggctgcaata
    16621 tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc gaatatttgc
    16681 ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat atgattttta
    16741 tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat taatagatta
    16801 cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt aactttttca
    16861 atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc cacttttctc
    16921 tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt ttcatccgct
    16981 tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc tttagaacgt
    17041 tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt gaattctaag
    17101 aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac ttcaagatgg
    17161 gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt tggcaataac
    17221 gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg tgccgcattt
    17281 tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt cacggttttt
    17341 ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac ataatgtaaa
    17401 tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc cacggaggct
    17461 tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac atttggtgtt
    17521 aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact attcaaagaa
    17581 tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat cagcatcaaa
    17641 ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt tgcatcaaag
    17701 aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga atactgatta
    17761 tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc tttgatcttc
    17821 gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat atattcactt
    17881 ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat cgcttcatta
    17941 gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt cgtgatcaaa
    18001 ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc actcatgcca
    18061 cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat gagaatacct
    18121 aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt actcttctgt
    18181 ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca atgaccaata
    18241 tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg attccttgtc
    18301 ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg acgaaaaatg
    18361 taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct atgattcctg
    18421 gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca ccaaggtctc
    18481 tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc atcaagacga
    18541 cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct aatccgaatc
    18601 ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga gagactaata
    18661 agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg taatagattc
    18721 ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa gccagcatga
    18781 acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca taattttccc
    18841 aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa aaaatcagat
    18901 agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac gaccatttaa
    18961 attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc atcacgatga
    19021 acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt tgtgtaacca
    19081 agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca atcagacgag
    19141 tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt ttcacaccat
    19201 ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg cctaaatctc
    19261 tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt ggcataatca
    19321 taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg aataatatct
    19381 gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc acactgaaaa
    19441 tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa ataatcagca
    19501 tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg taactgatga
    19561 tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc atagaaagcg
    19621 atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca ttcatatcaa
    19681 aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg agccacaaaa
    19741 tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct attcttcgta
    19801 gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga catggatatc
    19861 ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg ttgaatggac
    19921 catccattca ttttgctgtt catcataaca gatgatttca taatggacac cacgaaacag
    19981 ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg tcaaatcctc
    20041 tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga cacattcaaa
    20101 ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca cgatattgct
    20161 ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat ctacgggtgt
    20221 accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca ttgccaacgc
    20281 ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt gcagatcccg
    20341 cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg gttcatctaa
    20401 tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct gtctttgacc
    20461 accagacatt tcactaatct cccgtttttc gtatccagga agctggacca tacgcaaagc
    20521 atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca atccaaaagc
    20581 aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga ctgtatttac
    20641 ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga tgtccccttc
    20701 tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac agccggaagg
    20761 accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt ttaatactgt
    20821 ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc ccacgttttt
    20881 accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt cgttttttga
    20941 gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt cctgcttttg
    21001 ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca aaaataaacg
    21061 tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa gtcagcaaga
    21121 caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca tacccgtttt
    21181 cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga ttcgctgctt
    21241 cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa aagaatgttt
    21301 ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt gacaaatctg
    21361 tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt cttaacttct
    21421 caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt cctgtttact
    21481 accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa aatttttttt
    21541 gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt atttttcacc
    21601 taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta tggtatgata
    21661 aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt tcaggtaata
    21721 aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt ggctgaaaat
    21781 gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt ggcaatgagc
    21841 gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt atccggaact
    21901 gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc tactggtttt
    21961 gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc aggagactgg
    22021 ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg tacagcaggg
    22081 tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc cgataaaaaa
    22141 gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct gttacataaa
    22201 ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac atggctaaaa
    22261 agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac aacaaaagct
    22321 ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca gctaagcagt
    22381 cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc tgggggaatc
    22441 aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat tggaattgat
    22501 tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc ggatttgcag
    22561 attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga aacagcatct
    22621 aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga ccataaatga
    22681 agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt gctattacaa
    22741 atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc aatccggata
    22801 ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac tccaatataa
    22861 cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa ctaacgaaaa
    22921 aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact cctgctttta
    22981 gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa gaagaactgg
    23041 acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc tcttctaaca
    23101 ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat ctacgtgaat
    23161 ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca aaggaatctc
    23221 ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt ttcgcaaatg
    23281 atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa gatggaacag
    23341 tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa attagactgg
    23401 aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg cttacaaaag
    23461 cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga ttatgtctaa
    23521 aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc gcgcagtcca
    23581 tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac tagcaaaaca
    23641 agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc tagcaaacgg
    23701 cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa caccaaaatt
    23761 gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc gtaatctgga
    23821 aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt tagcttgtgg
    23881 agattttgga aaaggtgcct tagctgatat tcagacggtc atcgaacagg tcatgctgac
    23941 tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa aaaactttac
    24001 attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt taggttttat
    24061 ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg cttcgatcgt
    24121 tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca gcatgatccg
    24181 tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc cagtcatcgg
    24241 aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca tcttgattgg
    24301 ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa aagcagattc
    24361 cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt tagtgatgaa
    24421 tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa atttaaatgc
    24481 tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag gaatcgtcgc
    24541 aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc acccttctct
    24601 tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt gttactattg
    24661 aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg taggtgcaaa
    24721 cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg aaatttttgc
    24781 aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt cgtatgtctc
    24841 ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca tcgatgtatt
    24901 gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg atggcgcaat
    24961 caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg tcagtgctgt
    25021 ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata ttactaaaca
    25081 ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc aaccagtcac
    25141 attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg agaaggcatc
    25201 aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg aagaaacgga
    25261 tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata cagcattcca
    25321 agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat cctggttctg
    25381 tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc gaattactga
    25441 tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa tcagtctctt
    25501 tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt gagaataaaa
    25561 gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat ctaggtggtt
    25621 atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat acgctagact
    25681 atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca ctttactggc
    25741 ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt ggtgtgggaa
    25801 cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt actgcaggat
    25861 cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc aactataaaa
    25921 aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc gatgttattt
    25981 tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc gttgatggac
    26041 gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac cttggtgtat
    26101 tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt gatcagtata
    26161 aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa aatggcaaga
    26221 ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg catgaatata
    26281 tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa aatcatgctg
    26341 at
//
LOCUS       DACELF010000033        26271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.033, whole genome shotgun sequence.
ACCESSION   DACELF010000033 DACELF010000000
VERSION     DACELF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.033"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW687_001315"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW687_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0950586.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IW687_001316"
     CDS             complement(1551..2972)
                     /locus_tag="IW687_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0950587.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW687_001317"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW687_001317"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0950588.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IW687_001318"
     CDS             complement(5383..6639)
                     /locus_tag="IW687_001318"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0950589.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IW687_001319"
     CDS             7219..8520
                     /locus_tag="IW687_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0950590.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="IW687_001320"
     CDS             complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="IW687_001320"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0950591.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(10488..11087)
                     /locus_tag="IW687_001321"
     CDS             complement(10488..11087)
                     /locus_tag="IW687_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0950592.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            11341..12222
                     /locus_tag="IW687_001322"
     CDS             11341..12222
                     /locus_tag="IW687_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950593.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            12235..13845
                     /locus_tag="IW687_001323"
                     /pseudo
     CDS             12235..13845
                     /locus_tag="IW687_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(13922..14581)
                     /locus_tag="IW687_001324"
     CDS             complement(13922..14581)
                     /locus_tag="IW687_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0950594.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(14864..15529)
                     /locus_tag="IW687_001325"
     CDS             complement(14864..15529)
                     /locus_tag="IW687_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950595.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(15676..16281)
                     /gene="dhaL"
                     /locus_tag="IW687_001326"
     CDS             complement(15676..16281)
                     /gene="dhaL"
                     /locus_tag="IW687_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0950596.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(16309..17292)
                     /gene="dhaK"
                     /locus_tag="IW687_001327"
     CDS             complement(16309..17292)
                     /gene="dhaK"
                     /locus_tag="IW687_001327"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0950597.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(17294..17689)
                     /gene="dhaM"
                     /locus_tag="IW687_001328"
     CDS             complement(17294..17689)
                     /gene="dhaM"
                     /locus_tag="IW687_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0950598.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            17843..18163
                     /locus_tag="IW687_001329"
     CDS             17843..18163
                     /locus_tag="IW687_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0950599.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(18275..19144)
                     /locus_tag="IW687_001330"
     CDS             complement(18275..19144)
                     /locus_tag="IW687_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0950600.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(19240..20544)
                     /locus_tag="IW687_001331"
     CDS             complement(19240..20544)
                     /locus_tag="IW687_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0950601.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(20541..21050)
                     /locus_tag="IW687_001332"
     CDS             complement(20541..21050)
                     /locus_tag="IW687_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950602.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(21225..23360)
                     /locus_tag="IW687_001333"
     CDS             complement(21225..23360)
                     /locus_tag="IW687_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0950603.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(23443..25098)
                     /locus_tag="IW687_001334"
     CDS             complement(23443..25098)
                     /locus_tag="IW687_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0950604.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(25100..25414)
                     /locus_tag="IW687_001335"
     CDS             complement(25100..25414)
                     /locus_tag="IW687_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0950605.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            25584..25844
                     /locus_tag="IW687_001336"
     CDS             25584..25844
                     /locus_tag="IW687_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0950606.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            25858..26172
                     /locus_tag="IW687_001337"
     CDS             25858..26172
                     /locus_tag="IW687_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0950607.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         7374 a         5491 c         4752 g         8654 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaatc gagtagaggg gaaagccaac tgaccttccg cccctctcac
     8581 accaccgtac gtacggaccc gtatacggcg gttcaataag ttgagtgtac gaactggtac
     8641 ttcaaggaga tgtcctcata tccttggcgg gccagttctt tgtctgttaa tgatcgatga
     8701 agaatatgac ttttggagac tgaccaaata ccttttcttg tatttgacca ttcatacgct
     8761 ttcgctttat cgatccctaa acgaatcaga ttttttcttt tggttcttgg attcttccat
     8821 tgtttccaaa tatattgtct gattcttcgc ttcaaccaac cgtttaactc cctcatgaat
     8881 tctttcatct caccaattcc atagtagttg atccaaccag tcattagttg cttgatttct
     8941 tttaaaataa gttggatact tcttccacgg tttcttttag tgagttgttt gagcttgctt
     9001 ttcacacgct gttttgcttt tgcgtgaggg cgaattttaa tacctttctt tgttggcaga
     9061 atgcagaatc caagaaactt tcgtttcaat ggacttccta ccgcactctt ttctcgattt
     9121 accgtcaact ttagttgttc ttcgagaaat tgaatggatt tcttcatcac acgctgtcct
     9181 gctcgtttgc ttctaacata gatattgcag tcatcggcgt atcgaacaaa tttatgtcct
     9241 agattttcta gcacttgatc aaattcgttc aggtagatgt tacttaacag gggagagaga
     9301 ttgcctcctt ggggcgttcc ttcttcagtt gttgtgatga gtccattttc cattacacca
     9361 ctcattaagt attgacggat cagttttagg acttgtttgt cctttacttt ccgctcaatg
     9421 aaatacatca gcttatcgtg atttacggtg tcaaagtagg ctttcatatc aatgtccacc
     9481 acatatcggt aaccttgttc ataataagat tttgctttct tcactgcgtc atgtgcgctg
     9541 cgatttgggc gaaacccata actattatct gagaattctg gttcaaatag cggttgtaac
     9601 acttgattga ttgcctgttg aagcatacga tcaataaccg tgggaattcc tagttttctt
     9661 acaccgccgt ttggtttcgg tatttctact cgcaaaacag gctgaggttt atagttccct
     9721 tggcgtaaat tggatagtaa ttcctcgcgg tgctctttca agtaaggaag cagttgatcg
     9781 acggtcatcc catctgcacc agccgcccct ttatttctga ctacttgaag atacgcttga
     9841 ttgagattat tccgatctag aattctccca aacagatttt ctacaccatc ttgtttcctc
     9901 tgttcaccgc aagaagtgct gcgcacttca tcataccctt cgtgttccac actagctctt
     9961 tgatgatagc caagttttct tgttttctgc ggtcgtcgca tcttctcaca cctcctaaat
    10021 tattcaagat tcttattgtt cagtccttcg taacggtcag tgtcgttact actatgacct
    10081 cggctgactt ctcatgattc accatcatgt cgccatgatc gttgtgctgg tttgttttca
    10141 ttcaacgccc acaacaccct catgagatct cccccggtaa gagtgaaaac tttcctccca
    10201 tgtaactgct agatttactg tataggtttc gggcagtatt ggactttgtc ttgtgatgaa
    10261 gacttatccg acctaattca gccttgtatc tagtttctgt tcgtcagttc aggagtttgc
    10321 gtccgacttc cttcagccac aacctcacga ttgccgcctt gtctttcgct aacagttcct
    10381 actgccaagc ctgtagtgga ctttcaccac caagttttca cccatgcagg gcgcacacca
    10441 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    10501 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    10561 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    10621 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    10681 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    10741 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    10801 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    10861 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    10921 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    10981 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    11041 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    11101 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
    11161 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
    11221 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
    11281 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
    11341 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
    11401 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
    11461 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
    11521 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
    11581 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
    11641 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
    11701 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
    11761 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
    11821 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
    11881 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
    11941 gtgatcatac gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg
    12001 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
    12061 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
    12121 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
    12181 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
    12241 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
    12301 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
    12361 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
    12421 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
    12481 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
    12541 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
    12601 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
    12661 cttttagcaa tatcgatcag ttgtacgatg atttttttcc aagcggattc gatcactgca
    12721 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
    12781 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
    12841 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
    12901 atgtggaaac ctttaggctg gtcttatttg acctatccat ttacaactaa caactgaatt
    12961 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
    13021 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
    13081 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
    13141 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
    13201 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
    13261 gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca
    13321 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
    13381 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
    13441 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
    13501 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
    13561 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt
    13621 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
    13681 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
    13741 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
    13801 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
    13861 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
    13921 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
    13981 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
    14041 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
    14101 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
    14161 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
    14221 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
    14281 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
    14341 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
    14401 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
    14461 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
    14521 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
    14581 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
    14641 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
    14701 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
    14761 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
    14821 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
    14881 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
    14941 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
    15001 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
    15061 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
    15121 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
    15181 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
    15241 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
    15301 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
    15361 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
    15421 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
    15481 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
    15541 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
    15601 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
    15661 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
    15721 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
    15781 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
    15841 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
    15901 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
    15961 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
    16021 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
    16081 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
    16141 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
    16201 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
    16261 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
    16321 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
    16381 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
    16441 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
    16501 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
    16561 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
    16621 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
    16681 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
    16741 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
    16801 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
    16861 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
    16921 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
    16981 attcttaacg atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat
    17041 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
    17101 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
    17161 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
    17221 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
    17281 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
    17341 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
    17401 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
    17461 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
    17521 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
    17581 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
    17641 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
    17701 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
    17761 taataaattt ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta
    17821 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
    17881 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
    17941 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
    18001 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
    18061 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
    18121 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
    18181 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
    18241 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
    18301 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
    18361 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
    18421 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
    18481 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
    18541 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
    18601 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
    18661 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
    18721 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
    18781 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
    18841 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
    18901 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
    18961 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg
    19021 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
    19081 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
    19141 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
    19201 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
    19261 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
    19321 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
    19381 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
    19441 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
    19501 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
    19561 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
    19621 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
    19681 ttttgcccaa cgttttctgt gtcgatatag catcttcaac gtcacaggaa cttccatgaa
    19741 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
    19801 actgatttct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
    19861 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
    19921 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
    19981 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
    20041 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
    20101 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
    20161 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
    20221 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
    20281 attcaatttc gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc
    20341 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
    20401 cttatgtttg tatataaaca cataacacca gacaccaata aaccatacaa atccgcctaa
    20461 aatcggataa gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt
    20521 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    20581 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    20641 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    20701 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
    20761 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
    20821 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
    20881 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
    20941 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
    21001 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
    21061 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
    21121 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    21181 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    21241 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    21301 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    21361 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    21421 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    21481 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    21541 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    21601 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    21661 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    21721 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    21781 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    21841 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    21901 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    21961 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    22021 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    22081 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    22141 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    22201 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    22261 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    22321 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    22381 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    22441 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    22501 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    22561 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    22621 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    22681 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    22741 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    22801 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    22861 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    22921 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    22981 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    23041 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    23101 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    23161 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    23221 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    23281 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac
    23341 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag
    23401 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    23461 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    23521 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    23581 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    23641 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    23701 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    23761 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    23821 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    23881 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    23941 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    24001 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    24061 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    24121 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    24181 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    24241 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    24301 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    24361 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    24421 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    24481 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    24541 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    24601 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    24661 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    24721 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    24781 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    24841 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    24901 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    24961 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    25021 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    25081 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    25141 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    25201 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    25261 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    25321 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    25381 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    25441 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    25501 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    25561 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    25621 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    25681 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    25741 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    25801 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    25861 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    25921 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    25981 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    26041 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    26101 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    26161 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    26221 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa a
//
LOCUS       DACELF010000034        25944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.034, whole genome shotgun sequence.
ACCESSION   DACELF010000034 DACELF010000000
VERSION     DACELF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.034"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW687_001338"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW687_001338"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0950608.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW687_001339"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW687_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0950609.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="IW687_001340"
     CDS             complement(3549..4358)
                     /locus_tag="IW687_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0950610.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW687_001341"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW687_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0950611.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="IW687_001342"
     CDS             complement(7968..8654)
                     /locus_tag="IW687_001342"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0950612.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="IW687_001343"
     CDS             complement(9002..10783)
                     /locus_tag="IW687_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0950613.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="IW687_001344"
     CDS             complement(10809..11723)
                     /locus_tag="IW687_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950614.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="IW687_001345"
     CDS             complement(11750..12712)
                     /locus_tag="IW687_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950615.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="IW687_001346"
     CDS             complement(12712..13656)
                     /locus_tag="IW687_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950616.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="IW687_001347"
     CDS             complement(13657..14661)
                     /locus_tag="IW687_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950617.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW687_001348"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW687_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0950618.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW687_001349"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW687_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0950619.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW687_001350"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW687_001350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0950620.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="IW687_001351"
     CDS             complement(18496..20178)
                     /locus_tag="IW687_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0950621.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="IW687_001352"
     CDS             complement(20230..20592)
                     /locus_tag="IW687_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0950622.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="IW687_001353"
     CDS             20862..21050
                     /locus_tag="IW687_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0950623.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="IW687_001354"
     CDS             21288..23957
                     /locus_tag="IW687_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0950624.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="IW687_001355"
     CDS             complement(24048..24668)
                     /locus_tag="IW687_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0950625.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="IW687_001356"
     CDS             24780..25181
                     /locus_tag="IW687_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950626.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="IW687_001357"
     CDS             25316..25654
                     /locus_tag="IW687_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ0950627.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5500 c         4644 g         8774 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttcc
//
LOCUS       DACELF010000035        25674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.035, whole genome shotgun sequence.
ACCESSION   DACELF010000035 DACELF010000000
VERSION     DACELF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.035"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..514
                     /locus_tag="IW687_001358"
     CDS             170..514
                     /locus_tag="IW687_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0950628.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IW687_001359"
                     /pseudo
     CDS             515..934
                     /locus_tag="IW687_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IW687_001360"
     CDS             complement(1026..1376)
                     /locus_tag="IW687_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950629.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IW687_001361"
     CDS             complement(1342..2673)
                     /locus_tag="IW687_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0950630.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IW687_001362"
     CDS             2929..3495
                     /locus_tag="IW687_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0950631.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IW687_001363"
     tRNA            3640..3727
                     /locus_tag="IW687_001363"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IW687_001364"
     CDS             4061..5035
                     /locus_tag="IW687_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0950632.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IW687_001365"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IW687_001365"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0950633.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IW687_001366"
     CDS             complement(7450..7896)
                     /locus_tag="IW687_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0950634.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IW687_001367"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IW687_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0950635.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IW687_001368"
     CDS             8361..8879
                     /locus_tag="IW687_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0950636.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IW687_001369"
     CDS             complement(8956..9912)
                     /locus_tag="IW687_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950637.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IW687_001370"
     CDS             10078..10884
                     /locus_tag="IW687_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950638.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IW687_001371"
     CDS             10881..11870
                     /locus_tag="IW687_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0950639.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IW687_001372"
     CDS             11867..12871
                     /locus_tag="IW687_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0950640.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IW687_001373"
     CDS             13003..13545
                     /locus_tag="IW687_001373"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0950641.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IW687_001374"
     CDS             13565..14263
                     /locus_tag="IW687_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0950642.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IW687_001375"
     CDS             14284..14496
                     /locus_tag="IW687_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0950643.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IW687_001376"
     CDS             14496..15458
                     /locus_tag="IW687_001376"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0950644.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IW687_001377"
     CDS             15476..15871
                     /locus_tag="IW687_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0950645.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IW687_001378"
     CDS             16034..16399
                     /locus_tag="IW687_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0950646.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IW687_001379"
     CDS             16396..18207
                     /locus_tag="IW687_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0950647.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IW687_001380"
     CDS             complement(18269..18436)
                     /locus_tag="IW687_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0950648.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IW687_001381"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IW687_001381"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0950649.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IW687_001382"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IW687_001382"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0950650.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IW687_001383"
     CDS             21193..22173
                     /locus_tag="IW687_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0950651.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IW687_001384"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IW687_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0950652.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IW687_001385"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IW687_001385"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0950653.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IW687_001386"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IW687_001386"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0950654.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IW687_001387"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IW687_001387"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0950655.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5376 g         7574 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt ttttctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatt
//
LOCUS       DACELF010000036        25598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.036, whole genome shotgun sequence.
ACCESSION   DACELF010000036 DACELF010000000
VERSION     DACELF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.036"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW687_001388"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW687_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0950656.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW687_001389"
     CDS             808..1242
                     /locus_tag="IW687_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0950657.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW687_001390"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW687_001390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0950658.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW687_001391"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW687_001391"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0950659.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW687_001392"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW687_001392"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0950660.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW687_001393"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW687_001393"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0950661.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW687_001394"
     CDS             9513..10439
                     /locus_tag="IW687_001394"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0950662.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW687_001395"
     CDS             10621..11514
                     /locus_tag="IW687_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0950663.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW687_001396"
     CDS             11648..11773
                     /locus_tag="IW687_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0950664.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW687_001397"
     CDS             complement(11907..12539)
                     /locus_tag="IW687_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950665.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW687_001398"
     CDS             complement(12687..13229)
                     /locus_tag="IW687_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0950666.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW687_001399"
     CDS             13364..13753
                     /locus_tag="IW687_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950667.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW687_001400"
     CDS             13757..14521
                     /locus_tag="IW687_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0950668.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IW687_001401"
     CDS             14650..15597
                     /locus_tag="IW687_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0950669.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IW687_001402"
     CDS             15727..16467
                     /locus_tag="IW687_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950670.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IW687_001403"
     CDS             complement(16758..17426)
                     /locus_tag="IW687_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0950671.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IW687_001404"
     CDS             17560..17847
                     /locus_tag="IW687_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950672.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IW687_001405"
     CDS             18263..19801
                     /locus_tag="IW687_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0950673.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IW687_001406"
     CDS             19798..20763
                     /locus_tag="IW687_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950674.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IW687_001407"
     CDS             20750..22072
                     /locus_tag="IW687_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0950675.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IW687_001408"
     CDS             22313..23056
                     /locus_tag="IW687_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950676.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IW687_001409"
     CDS             23071..24063
                     /locus_tag="IW687_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950677.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IW687_001410"
     CDS             24075..24812
                     /locus_tag="IW687_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950678.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IW687_001411"
     CDS             24802..25524
                     /locus_tag="IW687_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0950679.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         8865 a         4337 c         5324 g         7072 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaat
//
LOCUS       DACELF010000037        25403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.037, whole genome shotgun sequence.
ACCESSION   DACELF010000037 DACELF010000000
VERSION     DACELF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.037"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW687_001412"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW687_001412"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0950680.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW687_001413"
     CDS             complement(937..1518)
                     /locus_tag="IW687_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0950681.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW687_001414"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW687_001414"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0950682.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW687_001415"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW687_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0950683.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW687_001416"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW687_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0950684.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW687_001417"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW687_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0950685.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW687_001418"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW687_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0950686.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW687_001419"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW687_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0950687.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW687_001420"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW687_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0950688.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW687_001421"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW687_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0950689.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW687_001422"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW687_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0950690.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW687_001423"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW687_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0950691.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW687_001424"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW687_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0950692.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW687_001425"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW687_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0950693.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW687_001426"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW687_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0950694.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW687_001427"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW687_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0950695.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW687_001428"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW687_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0950696.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW687_001429"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW687_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0950697.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW687_001430"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW687_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0950698.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW687_001431"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW687_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0950699.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW687_001432"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW687_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0950700.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW687_001433"
     CDS             13972..14157
                     /locus_tag="IW687_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0950701.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW687_001434"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW687_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0950702.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW687_001435"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW687_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0950703.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW687_001436"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW687_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0950704.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW687_001437"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW687_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0950705.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW687_001438"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW687_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0950706.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW687_001439"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW687_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0950707.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW687_001440"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW687_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0950708.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW687_001441"
     CDS             18232..18879
                     /locus_tag="IW687_001441"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0950709.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW687_001442"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW687_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0950710.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW687_001443"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW687_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0950711.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW687_001444"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW687_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0950712.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW687_001445"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW687_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0950713.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW687_001446"
     CDS             20317..21255
                     /locus_tag="IW687_001446"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0950714.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW687_001447"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW687_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0950715.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW687_001448"
     CDS             21935..22774
                     /locus_tag="IW687_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0950716.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW687_001449"
     CDS             22750..23619
                     /locus_tag="IW687_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0950717.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW687_001450"
     CDS             23609..24406
                     /locus_tag="IW687_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0950718.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW687_001451"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW687_001451"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0950719.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8249 a         4686 c         5474 g         6994 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tac
//
LOCUS       DACELF010000038        25378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.038, whole genome shotgun sequence.
ACCESSION   DACELF010000038 DACELF010000000
VERSION     DACELF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.038"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW687_001452"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW687_001452"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0950720.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IW687_001453"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IW687_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0950721.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IW687_001454"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IW687_001454"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0950722.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IW687_001455"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IW687_001455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0950723.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IW687_001456"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IW687_001456"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0950724.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IW687_001457"
     CDS             5265..5843
                     /locus_tag="IW687_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0950725.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IW687_001458"
     CDS             5895..6377
                     /locus_tag="IW687_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0950726.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IW687_001459"
     CDS             6494..6868
                     /locus_tag="IW687_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950727.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW687_001460"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW687_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0950728.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IW687_001461"
     CDS             9167..9757
                     /locus_tag="IW687_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0950729.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IW687_001462"
     CDS             complement(9885..11234)
                     /locus_tag="IW687_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0950730.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IW687_001463"
     CDS             complement(11402..12142)
                     /locus_tag="IW687_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950731.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IW687_001464"
     CDS             complement(12155..13747)
                     /locus_tag="IW687_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0950732.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IW687_001465"
     CDS             complement(14315..14611)
                     /locus_tag="IW687_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950733.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IW687_001466"
     CDS             14787..15512
                     /locus_tag="IW687_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0950734.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IW687_001467"
     CDS             15505..16347
                     /locus_tag="IW687_001467"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0950735.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IW687_001468"
     CDS             16350..16871
                     /locus_tag="IW687_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0950736.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IW687_001469"
     CDS             complement(17124..17687)
                     /locus_tag="IW687_001469"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0950737.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IW687_001470"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IW687_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0950738.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IW687_001471"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IW687_001471"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0950739.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21031..21297
                     /locus_tag="IW687_001472"
     CDS             21031..21297
                     /locus_tag="IW687_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0950740.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21441..23432
                     /locus_tag="IW687_001473"
     CDS             21441..23432
                     /locus_tag="IW687_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0950741.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23786..25087
                     /locus_tag="IW687_001474"
     CDS             23786..25087
                     /locus_tag="IW687_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0950742.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8173 a         4808 c         5203 g         7194 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaacga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt aggagctgtt
    20701 tttacttggc tgttcttatt acttgatgca gaacagcttt tttacaacct ctccaataaa
    20761 cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata tgaggaacta
    20821 tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac ctctgattca
    20881 cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac aacttctttt
    20941 tttctggagt ttcttggagc atatttttgg agtccttctg aataaagcgt aaaataaaag
    21001 tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc gttatttatc
    21061 ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc gggaggcgta
    21121 tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt actaggacca
    21181 ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc aattttttgc
    21241 cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa agaataattt
    21301 tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa tttaggaaag
    21361 cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga tatccatata
    21421 acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata tcaatggctg
    21481 gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg ctttacgtta
    21541 tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat tttgttatcg
    21601 gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa tatgtcgtga
    21661 ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac acacttgtcc
    21721 gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc ttactagcag
    21781 atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg cgaggaattc
    21841 cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc actttagtta
    21901 ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga aaagcattcg
    21961 gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg aacttcagtc
    22021 aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg ctttttagtg
    22081 cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg acaggtgcag
    22141 aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg agctggcctt
    22201 acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg ttgaatgttt
    22261 atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa atgatgcctc
    22321 aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt gcttcgcaag
    22381 cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa ctgctgcctc
    22441 ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca gcagtcaatc
    22501 tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct catcatatgg
    22561 aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca ctgctttact
    22621 tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc tttttcttcg
    22681 gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga ggcggttttg
    22741 tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa caagggaaca
    22801 ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg cagcttagag
    22861 aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc ttggttcctg
    22921 atatggctga agataaagtt ggccgacagt tcatctattc gattttagat aaacggccta
    22981 aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca tatacgaaag
    23041 aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg ttcttaggct
    23101 tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat ttgatgaaac
    23161 aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga gaagttggcg
    23221 atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta ggaaaatggc
    23281 aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg ccagaaacat
    23341 ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc attggaaata
    23401 ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg aactaatgtc
    23461 gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta ctgcaacaaa
    23521 cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg ggcttaatgc
    23581 cgcagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc tgtttagcaa
    23641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
    23701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
    23761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
    23821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
    23881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
    23941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
    24001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
    24061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
    24121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
    24181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
    24241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
    24301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
    24361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
    24421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
    24481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
    24541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
    24601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
    24661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
    24721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
    24781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
    24841 agatggctct tatattatct ccggcaaact aacattagat gatttccagc gttattttca
    24901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
    24961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
    25021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaac
    25081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttccta cgattagtca
    25141 agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa gcacaccaaa
    25201 taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac tgtaagtggt
    25261 ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt gccctaacca
    25321 tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc ttatcttt
//
LOCUS       DACELF010000039        25113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.039, whole genome shotgun sequence.
ACCESSION   DACELF010000039 DACELF010000000
VERSION     DACELF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.039"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            367..1278
                     /locus_tag="IW687_001475"
     CDS             367..1278
                     /locus_tag="IW687_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0950743.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1495..1932
                     /locus_tag="IW687_001476"
     CDS             1495..1932
                     /locus_tag="IW687_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950744.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2027..2911
                     /locus_tag="IW687_001477"
     CDS             2027..2911
                     /locus_tag="IW687_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0950745.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3135..6302
                     /locus_tag="IW687_001478"
     CDS             3135..6302
                     /locus_tag="IW687_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950746.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6322..6867
                     /locus_tag="IW687_001479"
     CDS             6322..6867
                     /locus_tag="IW687_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0950747.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6895..7350
                     /locus_tag="IW687_001480"
     CDS             6895..7350
                     /locus_tag="IW687_001480"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0950748.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7347..8105
                     /locus_tag="IW687_001481"
     CDS             7347..8105
                     /locus_tag="IW687_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0950749.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8191..8931)
                     /locus_tag="IW687_001482"
     CDS             complement(8191..8931)
                     /locus_tag="IW687_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0950750.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8992..10245
                     /locus_tag="IW687_001483"
     CDS             8992..10245
                     /locus_tag="IW687_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0950751.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10567..10794
                     /locus_tag="IW687_001484"
     CDS             10567..10794
                     /locus_tag="IW687_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0950752.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10917..11243
                     /locus_tag="IW687_001485"
     CDS             10917..11243
                     /locus_tag="IW687_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0950753.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11311..12024
                     /locus_tag="IW687_001486"
     CDS             11311..12024
                     /locus_tag="IW687_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0950754.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12334..13185
                     /locus_tag="IW687_001487"
     CDS             12334..13185
                     /locus_tag="IW687_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0950755.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13306..15171
                     /locus_tag="IW687_001488"
     CDS             13306..15171
                     /locus_tag="IW687_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0950756.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIIAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15219..16628
                     /locus_tag="IW687_001489"
     CDS             15219..16628
                     /locus_tag="IW687_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0950757.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16736..17437
                     /locus_tag="IW687_001490"
     CDS             16736..17437
                     /locus_tag="IW687_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0950758.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17596..18075
                     /locus_tag="IW687_001491"
                     /pseudo
     CDS             17596..18075
                     /locus_tag="IW687_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18923..19498
                     /locus_tag="IW687_001492"
     CDS             18923..19498
                     /locus_tag="IW687_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0950759.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19498..19938
                     /locus_tag="IW687_001493"
     CDS             19498..19938
                     /locus_tag="IW687_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0950760.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19954..20655
                     /locus_tag="IW687_001494"
     CDS             19954..20655
                     /locus_tag="IW687_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0950761.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21143..21568)
                     /locus_tag="IW687_001495"
     CDS             complement(21143..21568)
                     /locus_tag="IW687_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950762.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21942..22913
                     /locus_tag="IW687_001496"
     CDS             21942..22913
                     /locus_tag="IW687_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0950763.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22945..24342
                     /locus_tag="IW687_001497"
     CDS             22945..24342
                     /locus_tag="IW687_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0950764.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24501..25109
                     /locus_tag="IW687_001498"
     CDS             24501..25109
                     /locus_tag="IW687_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950765.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8573 a         4132 c         5295 g         7113 t
ORIGIN      
        1 tgtataaaaa tatatcataa taaaaacaac aaaaaaatgt ctggaaaatc gaacataaaa
       61 gtgtctgatt tgaatatccc agcagcggaa gaagaaaaac aaaatcgata acatttgttc
      121 taagaattag ttacgttaaa tacggaacgc aagatagact tgaaaaacgg taacaaaaga
      181 agtagtatat taaggtgtga tgtgctactt gacatttatt ctgctgagcg aactttctgg
      241 attgtgtagc cttatatcta ttcaggacgt atcgagcaga ttagtaaagg tgttcttgat
      301 gattttcatc ttgaacacct ttgtttttag caattcgcaa aaaacgaaaa gtaggagaat
      361 tgataaatga aaaaaagatt tttagcactt gccatcgtac taggcactgg cttattgtca
      421 ggctgtacga atgccggtga aaaaacagcc gttagttata aaggcggtac aatcagcgaa
      481 caagaagtaa tggatagctt gaaaaaaatg caaggagcag attccgctgt gcaacagttg
      541 atcgtttatc aagtatttga agataaatat ggagatgacg tatcaaccaa agaaatcgat
      601 tctcaatatg atcaaacaaa gaaacaattg ggtgattcat ttgattcaca attgaaatct
      661 gcaggttaca cagaacaaac attcaaagac agtatcaaac aaagtctagc ctttcaagaa
      721 ggtctaaaga aacatatcaa attgaccgat gaagatttaa aaacggcttg ggaatctttc
      781 catccagaag tggaagctca aattatccaa gtagcaagtg aagatgatgc aaaagatgtg
      841 aagaaagctg ctgataaggg agacgatttc tctaaattag ccaaagacaa gtcaactgat
      901 actacgacaa aagaagatgg cggaaaagtg aaatttgatt ccactactac aacggttccg
      961 gcagaagtca aagaagcagc ctttaagttg aaagatggtc aagtttctga tgtgatcact
     1021 tctaccaatg cttcaactta tactacggaa tattatgtgg ttaaaatggt gaaaaaccaa
     1081 aacaaaggca acgacatgga taaatacaaa aaagaattga aagaaattgc aacagacaca
     1141 aaattaagtg acagtacctt ccagaataaa gtaattggcg aagtattgaa agacgctaat
     1201 gtaaaaatca aagacaaaga ctttgaaaat gttttgagta cattcacttc agattctagt
     1261 acagcaagta gtaaataagc ggtataaata aaaaatatga actatttaaa tcaataaaac
     1321 tgttcatcta aatcttttta ttagaactaa atcaacagta gtttaattac tacttgattt
     1381 agttcttttt attttaaaaa ttttttttac ttagaatcca gcgaccctag atatttttct
     1441 gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag
     1501 tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc
     1561 acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt
     1621 tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc
     1681 cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc
     1741 gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg
     1801 tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct
     1861 tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt
     1921 gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt
     1981 atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt
     2041 attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat
     2101 agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga
     2161 tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg
     2221 cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa
     2281 tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct
     2341 agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg
     2401 aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat
     2461 caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg
     2521 aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa
     2581 ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga
     2641 aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga
     2701 ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc
     2761 tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga
     2821 agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt
     2881 attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg
     2941 gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt
     3001 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
     3061 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
     3121 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     3181 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
     3241 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
     3301 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
     3361 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
     3421 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
     3481 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
     3541 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
     3601 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
     3661 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
     3721 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt
     3781 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
     3841 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
     3901 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
     3961 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     4021 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     4081 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     4141 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     4201 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     4261 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     4321 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     4381 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     4441 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     4501 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     4561 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     4621 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     4681 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     4741 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     4801 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     4861 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     4921 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     4981 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     5041 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     5101 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     5161 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     5221 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     5281 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     5341 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     5401 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     5461 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     5521 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     5581 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     5641 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     5701 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     5761 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     5821 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     5881 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     5941 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     6001 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     6061 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     6121 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     6181 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     6241 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     6301 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     6361 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     6421 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     6481 tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac
     6541 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     6601 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     6661 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     6721 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     6781 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     6841 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     6901 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     6961 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     7021 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     7081 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     7141 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     7201 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     7261 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     7321 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     7381 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     7441 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     7501 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     7561 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     7621 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     7681 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     7741 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     7801 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     7861 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     7921 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     7981 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     8041 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     8101 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     8161 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     8221 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     8281 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     8341 agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga
     8401 agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt
     8461 gatactttca atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc
     8521 aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac
     8581 ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt
     8641 ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg
     8701 tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc
     8761 gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat
     8821 ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat
     8881 gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct
     8941 cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca
     9001 acagtgacac tttatttgac ctttactttg attttagatt taggtatcta tttattcttt
     9061 tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt
     9121 ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt
     9181 ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct
     9241 atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat
     9301 cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa
     9361 agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat
     9421 tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt
     9481 cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt
     9541 tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa
     9601 tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat
     9661 gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg
     9721 ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata
     9781 caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa
     9841 aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc
     9901 ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat
     9961 catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca
    10021 cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa
    10081 agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg
    10141 gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt
    10201 gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga
    10261 taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac
    10321 agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag
    10381 ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa
    10441 gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa
    10501 agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca
    10561 aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa
    10621 gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta
    10681 agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc
    10741 tcaagggaag aagacacgct actgcataca catggtggct acctaaatta ttgattttct
    10801 atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt
    10861 atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga
    10921 atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg
    10981 aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat
    11041 ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag
    11101 atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca
    11161 ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac
    11221 gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa
    11281 aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg
    11341 ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa
    11401 cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca
    11461 aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa
    11521 ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt
    11581 gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat
    11641 acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat
    11701 tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat
    11761 ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca
    11821 ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa
    11881 gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa
    11941 ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc
    12001 aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag
    12061 ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga
    12121 ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt
    12181 atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg
    12241 attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag
    12301 gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac
    12361 aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta
    12421 gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt
    12481 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
    12541 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
    12601 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
    12661 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
    12721 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
    12781 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
    12841 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
    12901 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
    12961 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    13021 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    13081 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    13141 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    13201 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    13261 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
    13321 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
    13381 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
    13441 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
    13501 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
    13561 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
    13621 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
    13681 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
    13741 gaagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
    13801 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
    13861 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
    13921 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
    13981 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
    14041 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
    14101 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
    14161 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca tcggggattc
    14221 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
    14281 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
    14341 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
    14401 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
    14461 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
    14521 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
    14581 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
    14641 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
    14701 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
    14761 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
    14821 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
    14881 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
    14941 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
    15001 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattatagc agctggctat
    15061 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
    15121 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
    15181 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
    15241 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
    15301 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    15361 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
    15421 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    15481 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    15541 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    15601 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    15661 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
    15721 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    15781 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    15841 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    15901 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    15961 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    16021 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    16081 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    16141 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    16201 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    16261 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    16321 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    16381 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    16441 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
    16501 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    16561 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    16621 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    16681 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    16741 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    16801 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
    16861 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    16921 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    16981 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    17041 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    17101 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    17161 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    17221 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    17281 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
    17341 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    17401 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    17461 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    17521 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
    17581 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
    17641 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
    17701 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
    17761 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
    17821 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
    17881 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
    17941 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
    18001 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
    18061 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
    18121 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
    18181 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
    18241 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
    18301 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
    18361 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
    18421 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
    18481 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
    18541 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat
    18601 ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta
    18661 tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag tatcttctat
    18721 aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg
    18781 gctgaaaaat gatttttttc taaatactat atatagtggc gtctatccta tataccacta
    18841 aaaaactaca aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt
    18901 ttgagttagg gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa
    18961 taaagaaagt tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat
    19021 cgaactggtt tacggtggtt catactccgg gattatggga gtgattagta aaacagtact
    19081 ggaaaataac ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc
    19141 tggaaaagat gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt
    19201 atttgaaaag ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt
    19261 ctcacaactt ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt
    19321 gaatattggc ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga
    19381 ttttatggat aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt
    19441 caatgaatta cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg
    19501 agtcaaatat atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt
    19561 atcgaaaaag cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac
    19621 caagaaagca attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat
    19681 atggaagaac tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca
    19741 atcgatccag gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc
    19801 attgtacaag aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat
    19861 ttaaaatcag accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac
    19921 gtaggcgagt acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg
    19981 attggtgcag gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca
    20041 ttttatgaaa atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag
    20101 tacgcttttt tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct
    20161 ttagtgaacg atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat
    20221 ctaaacgctg atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta
    20281 gatacgatgc taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc
    20341 catatcaaag tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat
    20401 gaacagctag aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat
    20461 aatcaatggt atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc
    20521 gattttgtag aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta
    20581 gctgaattgg aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt
    20641 caagaattag gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt
    20701 agaataagcc tttctatcgt ttggttattt tatattcaaa tatttgaaat aaataaatct
    20761 ttactctttg atttgtaaac tgaaatgatc cactgtttgt tttactgatg aagcttgatc
    20821 aaaggaagaa gctgagtttg aagttcataa aaatcagcca tagtgagttt tgcttggatt
    20881 tgtttatctt cttctacttt ttacataatt ttgaacagga taagattcaa atgttttaaa
    20941 tgaatctttg actagtttgt gacagttgtc gcatatgctc aagcttcctt ggtcattaaa
    21001 ttctgtatgg atatttttgt tgttattaca tgtacaataa tcaggactat cgatcatcca
    21061 tttcgtctcc tttacaatca tcttgcttct ttactatagc agaaaagaag gggagaaaca
    21121 aaagaagatc caacagacgg cactatagac tgtagatgtt gccatcctca tccttcaaac
    21181 ggtaattacc gatttttgta aatatgactg ttgtttcttt tttattttct tttcctgaaa
    21241 taatatattc gtcatctttg gtagtgatct tgatatctgt cagcactgtt ttttcttcag
    21301 atgattcaga agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc atttttttat
    21361 ctgtaaatgt caactgtccc gcggataaat ccatatcttt acgtactttt agcgtatata
    21421 cactgttggt ggtatctgct ttttccgctt ttaagacaga agaatcggtt gtttcgtccg
    21481 aggattggtc agagccgcca aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca
    21541 aaataaatag tacgattaat ttcttcaaaa tacttccccc atttgatagg ataaagaaat
    21601 tataccattg gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca aaacacccac
    21661 aaatagagta aattttagaa taaaatttta aaaaggcaaa aaatccgcca ctttcagaaa
    21721 gcgttttaaa aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat gaaccggttc
    21781 aaatacttgt ttaagtataa ttaatagtga gaggaagttg ctctactgaa gactttagtt
    21841 atatggatat tgatgacaag caacactatg caaagaatgc ggtcatcata atcgaacaac
    21901 aagaaagaga aatcaaatgt aactggcagg agtgagaatg tatggcatcg atccgtgatg
    21961 tggctaagat ggctggtgtt tcagtgggaa cagtttcccg ttacttgaat ggtcagcaac
    22021 tgaaggaaaa gaacatgaaa aaaatagcag aagcgatttc agcgttggac tataaagaaa
    22081 acatcattgc taaagggttg aagaataatc gaagcttctc tattggactt ttgataaacg
    22141 ggatttccag ccgttttggg tcggaagttg tctcagggat cgaaagagta gctgaagaaa
    22201 atgggtatag cttattatta agcgggtttg ccgatcagcc agaaaaaatt gaacaaaaaa
    22261 ttgaatacct gatgaagcat gtgatcgatg ggctgattgt cttcttatcc gaagaagagt
    22321 ggtctggatt tgaaatgctc acaaaaatga gtattccagt tatcgcattg aattgtccag
    22381 aaggaccagc aggtattgat acaatattag tgaatgatcg agaaagtgta gcaaaagtga
    22441 tcgctcatca tggtgaacaa ggacataaaa aaatcggatt tattgctgcc actcaaacag
    22501 actacgtagc aagagaacgt ttagcaggag ccaaagaagc ggtccaaaat gatccatcga
    22561 tccaattaga ggtctttaca ggagactatt ctagaaaaag cggctattac ggggcaaaag
    22621 cattattaga aaaaggggca actgctattt ttgtcagcaa ttacaatatg tcgattggtg
    22681 ccatcgaatg tttcaatcaa gaaggtgtca gaatcgggaa ggatattgcg ttcagtcact
    22741 atgattattt agataaaaac gatctgagta tccttccgaa aatcacgatc acgccaccaa
    22801 cagataaaat cggggaacga gcagcagaac agctgttaaa acgaatcaaa aaagaagagg
    22861 aagaaaatga aaaagtgatt gtgatggaaa atatcataca tggaatcaac taaaacgaac
    22921 taaaaaagaa aaagaggtgc tttgatgaaa aaatatcaat ttccagcaaa tttttggtgg
    22981 ggttctgcag caagtggccc acaaacagaa ggaaggattt caggcgatgg aaaaggagag
    23041 aacatttttg attattggta caaaaaagaa ccgcaaaagt tctttgatca agtaggacct
    23101 gaaaaaacct cgcaagttta tacgaagtat caagaggatg tacagttgat gaaacagact
    23161 gggcataatt catttcgaac atctatccaa tggagccgtt tgatacctga tgaatctgga
    23221 acaatcaacg aagaagccgt tcgtttctat catcgttatt ttgatgagtt gattgaaaac
    23281 ggtatcgaac cctttgtgaa tctgtatcat ttcgatatgc cgatgtatct gcaagaaaaa
    23341 ggcggatggt tgaaccgaga aacggttgaa gcctatgaaa aatatgccaa aagttgtttt
    23401 gaattgtttg gttcgaaagt aaaaaaatgg tttactcata atgaaccaat cgttccagtc
    23461 gaagcaggtt atttatatcg atggcattat ccagaagagt cagatatgaa aaaagcgatc
    23521 caagttggct atcatgaagc gttggcttca gcgttagcca tcaaatctta ccatgaaatg
    23581 gagcaaggtg gtgttattgg tatcatcttg aatctgacac caagctatcc aagagacgaa
    23641 tcaaaccctg aagatgttaa agcagcgcaa atagcagatg cgttcttcaa tcgttcattc
    23701 cttgatccag ctgtaaaagg cgagtttcca agtgaattga ttgagatagt aaaagaactt
    23761 gatctcatgc ctgtgatcca tgcagaggat ttgacggtta tcaaagaaaa tacagtagat
    23821 cttttaggta tcaattatta ccagccacgc cgggtaaaag caaaagaaac accaatcgat
    23881 actaaaaacg gtccaatgcc tgaggattat ttcgacaatt atgaaatgcc tggtcgtaaa
    23941 atgaatcctt atcgcggatg ggagatctat gaacaaggaa tctatgatat tttgatcaat
    24001 gtacgagatc attatggaaa tatcgactgc tttatttctg aaaacggtat gggtgtagaa
    24061 aacgaagcac gatttatcaa agaagatggc atgatccacg atgattatcg gatcgaattt
    24121 gtccaatctc atctgaaata tgtccatcaa gccattcaag aaggtgccaa ttgtttaggc
    24181 tatcatatgt ggacatgtat ggataactgg tcgtggacca atgcatataa gaatcgatat
    24241 ggctttattt cagtagactt agcacaagat ggaaaaagaa caatcaaaaa atcgggtcat
    24301 tggttcaaag acgtatcaga taaaaacgga tttgaggctt aagaaagaat agttgagagc
    24361 aaatgcgtga aattctccat tcttaccatt ttgtgaaaaa gaagttcaat tatttacgat
    24421 ctttttcaac ttttttgtct ttctatttca ttttttgtat ataatagaat agtcaaaatt
    24481 aaataggagt ggaggaaagc atgaggaaaa aaagaccgtt tcttctcttt tggttattcg
    24541 gttttttctt actgcttatt ttatccgcag cattttcagg aaaaatgaca gcagatcttg
    24601 ctcagaaaga aacagaaaaa atcgtagcaa gcaaagaatc agtagataat ttcaaaaaag
    24661 aattctcaac ttattggtgg gcggagtata aaataactgc caattacgaa aaaggcgaat
    24721 tggacattta tttgccaaaa ggaaaagaag aaaatcaatt tattaatatc gatgacgtac
    24781 ttagtgcgat ttctctcaga caatatcaat ttaaagacaa gcatttgacc gttttgcttt
    24841 tgactcatga tggcgacaaa gtagcccgtt tgatcaccgc ttattctgaa aaagtagaca
    24901 aaaaagtggt cgccgatcgt ttagcggaat ggaaaaagac aaatgaacaa gtcaagaaaa
    24961 caactgtctc ttctcaaaaa aacgagtcga caggaacaag tccttcagat tctggcagtg
    25021 gacaaacgat aacgggaagt gaagaaaatc aagcctcaat tattacgaat gagtcatcac
    25081 aaggacaata tcaatcctat ggacaatgaa aaa
//
LOCUS       DACELF010000040        24664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.040, whole genome shotgun sequence.
ACCESSION   DACELF010000040 DACELF010000000
VERSION     DACELF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.040"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            235..1041
                     /locus_tag="IW687_001499"
     CDS             235..1041
                     /locus_tag="IW687_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0950766.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1053..2273
                     /locus_tag="IW687_001500"
     CDS             1053..2273
                     /locus_tag="IW687_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950767.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2263..3849
                     /locus_tag="IW687_001501"
     CDS             2263..3849
                     /locus_tag="IW687_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0950768.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3888..6026
                     /locus_tag="IW687_001502"
     CDS             3888..6026
                     /locus_tag="IW687_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0950769.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6314..7705
                     /locus_tag="IW687_001503"
     CDS             6314..7705
                     /locus_tag="IW687_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0950770.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7764..8573
                     /locus_tag="IW687_001504"
     CDS             7764..8573
                     /locus_tag="IW687_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0950771.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8545..9534
                     /locus_tag="IW687_001505"
     CDS             8545..9534
                     /locus_tag="IW687_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0950772.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9648..10643
                     /locus_tag="IW687_001506"
     CDS             9648..10643
                     /locus_tag="IW687_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0950773.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10647..11789
                     /locus_tag="IW687_001507"
     CDS             10647..11789
                     /locus_tag="IW687_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ0950774.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11786..13213
                     /locus_tag="IW687_001508"
     CDS             11786..13213
                     /locus_tag="IW687_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0950775.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13280..14569)
                     /locus_tag="IW687_001509"
                     /pseudo
     CDS             complement(13280..14569)
                     /locus_tag="IW687_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14749..15159)
                     /gene="tagD"
                     /locus_tag="IW687_001510"
     CDS             complement(14749..15159)
                     /gene="tagD"
                     /locus_tag="IW687_001510"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ0950776.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15285..16676
                     /locus_tag="IW687_001511"
     CDS             15285..16676
                     /locus_tag="IW687_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ0950777.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16809..17756
                     /locus_tag="IW687_001512"
     CDS             16809..17756
                     /locus_tag="IW687_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0950778.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17796..18956)
                     /locus_tag="IW687_001513"
     CDS             complement(17796..18956)
                     /locus_tag="IW687_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0950779.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19121..20296
                     /gene="mltG"
                     /locus_tag="IW687_001514"
     CDS             19121..20296
                     /gene="mltG"
                     /locus_tag="IW687_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0950780.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20432..20911
                     /gene="greA"
                     /locus_tag="IW687_001515"
     CDS             20432..20911
                     /gene="greA"
                     /locus_tag="IW687_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0950781.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21079..21810
                     /locus_tag="IW687_001516"
     CDS             21079..21810
                     /locus_tag="IW687_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0950782.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21807..22874
                     /locus_tag="IW687_001517"
     CDS             21807..22874
                     /locus_tag="IW687_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0950783.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22881..23513
                     /locus_tag="IW687_001518"
     CDS             22881..23513
                     /locus_tag="IW687_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0950784.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23653..24318
                     /locus_tag="IW687_001519"
     CDS             23653..24318
                     /locus_tag="IW687_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0950785.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24346..24639
                     /locus_tag="IW687_001520"
     CDS             24346..24639
                     /locus_tag="IW687_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0950786.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8417 a         3663 c         4781 g         7803 t
ORIGIN      
        1 aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaccaacac aaaatattat
       61 agaacccctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag
      121 agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg gtaccaaaaa
      181 tatggtaaaa tggcacttga ctgtaattca ctgaaatcgc ttggaggaac acaaatgttt
      241 aaagatttgt atttatttat gaaagacatt tatcagaata gaaaattact gctacagttt
      301 tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt
      361 atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca
      421 caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg
      481 ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta
      541 gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg
      601 tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat
      661 ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca
      721 ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt
      781 acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt
      841 gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat
      901 cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc
      961 tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca
     1021 catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag
     1081 atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca
     1141 atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa
     1201 taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt
     1261 tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa
     1321 tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata
     1381 tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg
     1441 atatcattga atttgctgat attggcgacc atatttatca acctgtaaaa acctattcaa
     1501 gtgggatgtt cgttcgtcta gcttttgctg tagcgatcaa tgtgaatcct gatattttga
     1561 ttgtggatga agcattggca gtaggagatc ttgaattcca actgaaatgt atggaaaaat
     1621 ttacagaaat taagaactcg ggaaaaacga ttttattcgt ttctcatgat attagttcga
     1681 ttcgccgttt ttgtgatcgt tcctattggt tgaaaaatgg agaagttgtc gaatatggtg
     1741 atacgatgga agtaacgacg aattacgaaa actttttgaa gaaaaaatca gttaaaacag
     1801 tagatcgaaa taaaaaccaa gtagaacgct tcgcagctcc agatatcgta gacgtgacga
     1861 aagccgaatt gttggataaa aataaaaatc cagtggatat gctggaacaa ggagaaaaac
     1921 tatacgttaa agtaacctat gaagtaaaag acgatacaat caaaaatcca gttttaggaa
     1981 ttgctctgcg tacagtcgac aatttttatg tgtgcggact caatactcta ctagatggta
     2041 tcaagatccc ttggaaaaaa ggtgagaata tcttttatct ggagtatgaa aagttaggtt
     2101 tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt
     2161 tggtatacaa gacaaaatac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca
     2221 tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa
     2281 atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt
     2341 aaagttttag agtttggtcc cggaaacggt cgattaacga aacatttgat cggagcaaaa
     2401 caatgtgaag tgagcattgt tgagctagat aaggaattat tcgattttgt cagcgaattt
     2461 gcccaagatg gattttatgg agatatcgaa agttttgaat gggcaaacta ttatgctgga
     2521 cagacatttg attacgtctt gtttgcggat gttttagaac atttagtaga tcctggaaaa
     2581 acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct
     2641 aacttagcac ataattctgt catgatcgat ttgttcaata atcaattgcc ttgggcttct
     2701 tatggtctcc tagatgaaac ccataattct ttttatactc atgatggttt ccaaaaagtt
     2761 tttgaaaaag ccggattatt cattaatatt gaagattact tgtacttggc tgtaggagat
     2821 acagaattaa aatcaactta tgaagagttg ccggaagctg tacgctatga tttcaaaatg
     2881 cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagtacaa
     2941 acttccattg cacagccaca aaactctaat tatgtaaaag tactagaagt aacccagcaa
     3001 acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag
     3061 acactaagtt tccccgtgtc agaagcaaca gaaagaatgg tgtttcgctt cgcacagcaa
     3121 cctagcttta tcgaattcag tgcagaagct gctggagaaa aactcatatt tatcgattca
     3181 aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga aataccagag
     3241 ttcgctctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc
     3301 ggggaattga caccaacaat gaaagaattg ctggaaacaa ttcgtcctat ggctgaagtc
     3361 acaaagcagc tttctgagaa aaatgatgaa ttagaaagag aaaatcagca tcttcttgag
     3421 gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg
     3481 gaaagtgatg aatggacgat caaacataga ctaggacgac gtaagaaaaa aacatcgaaa
     3541 aaaatacaag aaaaagagct ttctatatgt attgatgaga aaatatggga tgcagaaaca
     3601 aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc attgtcctat
     3661 aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agatgaagtc
     3721 aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgaattacg aatcctttgc
     3781 gagcaagaac gttcattctt agtggaagca gtagctcaaa atggtcaatc atggatcatt
     3841 gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag
     3901 aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg
     3961 tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa
     4021 atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg
     4081 atgttaagac ccaaagcggt tttaagatac gtttatccgg gaaaatgaga ggaaaagcga
     4141 tcttagattt tcaaacggca aaacatcaaa atggcatagc tgtgaaacta aatggtaaat
     4201 acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag
     4261 ggataaatta tgcaaggact catggcgtaa aaaaaacaat ccgccgggtc aaactggaat
     4321 tgaaaccagg atcgatcgac tatgcagagt ggatcagtcg tcacgagaaa cctgatctga
     4381 aagagcaaag gaaagaagca caagggtttg cataccgtcc attgatttcc atacttatgc
     4441 ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ttggatcaaa
     4501 cgtatgatca ctgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa
     4561 agtgtctgga atcctatcaa gcaaaggatg atagaatcaa agttgttttc cgacaagaaa
     4621 atggacatat cagtctggca actaattctg cgttggaaat ggcagaagga gaatttatag
     4681 cgctcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc
     4741 taaacataca tccagaactg gatttgatct acagtgacga agataaaatc gatgcagatg
     4801 gcaatcgctt tgatccgcac ttcaaagcag actggtctcc cgatacattg atgggaaata
     4861 attatatctc tcacttaggc gtatatcgaa caagtattgt gaaaaagctt ggcggtttcc
     4921 gtaaaggcta cgaaggctct caagattatg atttggtcct tcgtgtgaca gaacaaatcc
     4981 ctgcagatca tatttaccat attgaccgag tcttgtatca ttggcggacg atcccaggat
     5041 caaccgcaag taatggtgaa gccaaaagtt atatctatga ttctggagtg aaagcattga
     5101 cagatgccct gtctagaagg aatatcaaag gaagcgtacg tcctggcaga atttctggat
     5161 tttatgagat tgcttatgac gtcttgcaag aggatttggt cagcgtgatc attccaacga
     5221 agaatggcta tgaagatcta aaaacttgtg tggattcgat tatcgaaaaa acgtcttatc
     5281 caaattatga aatcattatt gcagataatg gaagtacaga tcctaaaatg caggaattat
     5341 ttgcagaata caagcatcag ctgaaagacc gatttatcgt tgaattaatt gacattccat
     5401 tcaattattc acgtatcaac aatctggcag cagaaaaagc aaacggcaaa tactttttat
     5461 tcttgaataa cgatacagaa gtgattgaac ctgactggat gacagcgatg gtttcttatg
     5521 cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc
     5581 agcatgcagg agtattatta ggaatcggtg gtgttgcagg acatgcattg aataactatg
     5641 atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca
     5701 ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgtcaatgga tttgatgaaa
     5761 cattagaagt agcattcaat gatgttgact tgtgtttgaa agtttatgaa ctgggtcgtt
     5821 acaatgtcta tgcacatcaa gcagaattgt atcatttcga atctaaatca agaggttatg
     5881 aagatacacc agaaaaacaa agacgttttg ccggggaaat aaaaaaaatg caagataagt
     5941 ggccttctta tattgctcat gacccatttt ataacgataa tctaactaaa gaaggaatcg
     6001 gcgatttttc attgagacct gattgattaa aagaactgta ttttcatttt tattgatgaa
     6061 aatacagttt ttttagtata tgaaaattgt aacttgtgac cgaagagatg ataggaagaa
     6121 cacccattca gtaggaagaa tgtgatacga tttttgtcca actctctgtt aggcagaaat
     6181 atacatataa acatacaata gtcacctttt ctagtttctt ttaatttatt ttacaatttg
     6241 gttattcact aggtttttaa actttgctat ggtaagatga tagaggcatt attttatggt
     6301 taggagtgta tatatgaata aaaatgggga gtggaatgcg gcacgtagga tattcattat
     6361 catcatggat gtacttgtat tgaatgtgtc gatctatctt agctttttgc tgaagttcca
     6421 aggagaagtc ccagcaagaa acttagagac ctttcagcat tcagcagttt ttatttccat
     6481 tatctttatt gcgttgaata ttttgttagg agcttatgta ttctatagcc gagtgatcag
     6541 tgatatcgtc ttcattactg ttattggaca ggtgctgata gcattgggga tcatggtagt
     6601 tacttttgct ggacgttggt ttgcatttcc gcgtggcgtc atcgtgctct cgtttatact
     6661 tggcacgata ttacttagcg tttatcggat agttgtttat aaactatact taagagtcag
     6721 tcgtgataag aaagtcctta tcgtaggttt ggagaaagat gtcgcaccag ctatccaaaa
     6781 ctttcaatct aagaaaaaca acaaacataa agttgaagca gtagtgatcg acaagtatta
     6841 cgaaaacaca aaaaaattga ttgatgatat cgatattgtc tacttggctt ctaaaatccc
     6901 agaagacgaa aaaatcaata tttatcaatt agtaactaaa aaagagaaga agctattttt
     6961 aaatacaact tttgaaaatt taacgatgat caatccgaac atcatgaatt tcgaagatga
     7021 aagtatcatt gaagcttccg gtttcaaaat tcagccggaa tatgaattat tcaaacgtct
     7081 ttttgatatc atcgtttcgc ttattttact gattcttgct tcacctttta tgctaatcac
     7141 agcgattttg gtaaaaacaa cttcacctgg accaattatt tataaacaag tacgtattac
     7201 gaaaaatcaa aaggaatttt caatctataa atttcgttcc atgagtgtaa cagctgaagc
     7261 aaaatcagga ccggtcttgg caaagagcaa tgatgcacgt gtgacaccag taggcaaatt
     7321 tatccgtgcc gtacgttttg acgaattacc tcaaatcttt aatgtattga aaggcgatat
     7381 gtcgatcgtc ggaccacgtc cagaaagacc atttttcgtt gatcagttca atgaagaaaa
     7441 tccttattat tatttaagac acaatgtacg tgcagggatt accggatatg cacaagtata
     7501 cggaaaatat gtatcagatt ataatagtaa actacgtttt gatttgttgt atatcaaaaa
     7561 gtattcctta ctattagatt tgaagatttt acttcaaaca atcaaaatct tatttgataa
     7621 gatgtcctca caaggactag atgaagatga aaatatccgt tcgttagcag aaatcgactt
     7681 ttcacaagag aaaatttatc gctagatggg aaaaattttg agcaaagaag taggagagaa
     7741 cattggaaaa taaaaatata agtatgctgc tttctttata tgataaagaa aaaccagagt
     7801 atttaaagga agcattaact agtatttttg agcaaacagt aatgccaaaa gaaattgtat
     7861 tagtttatga cggccctata aatgaaaaac ttcaagtagt tgtcacttat ttccaacaaa
     7921 aagatcctca cttgtttaca gttgtaaaat tagaaaaaaa tcaaggattg gggattgctt
     7981 tagcaattgg attagaacat acgaaaaatc gcttagtagc cagaatggat acagatgata
     8041 tcatggaacc ccaacgatta gaaaaacaat tagagatctt ccaaaaagat ccagaaatag
     8101 cgattgttgg gagtaatatt gaggaattta ctggcacgct tagcaatgtt atcggaagaa
     8161 ggattgtacc tgaatataat gatgaaatcc gtgtgttttc gaaaagaaga aatcccttta
     8221 atcatatgac agtcatgttt gataaaaatg ctattttaga agtgggaaac tatcgcccat
     8281 taattggatt tgaagattat tatttgtggg taagactgtt gaaggcggga tataaagggt
     8341 ataatatcca agagattcta gtccatgcta gagcaggtgc tgacatgtat gctagacgtg
     8401 gaggaagtaa atatctttta ccaggaatta aaggccgata tcgcgtatgg aaagatggtc
     8461 taggatcact aaaagatttt ctgatagtta tttgcggaca tcttgtaatt agtttattac
     8521 cgaataaaat taggggaaaa gtatatgaat caaaacttag aaaaacaaac taaactttct
     8581 ataatcatac ctttttacaa tgcaaaaaat actttaaaaa gagctgtaga cagcgtctta
     8641 acgcaaggtt atcccaatat tgaaatcttg ttaattgatg atggatctac agatggtagt
     8701 agtgacattg gtgacgaaat tgctaatcta gagagtaaag tagcatgtta tcacttggaa
     8761 aacgctggtc catcagttgc taggaattgt gggataaaag aagcaactgg aaatttggta
     8821 ggatttttag atagtgacga ttatttttta cctgattgtt ttaataatgt tttgaaagac
     8881 taccatagga attaccccga tatcattgct tttggattaa gtaaaggaaa aaatatagaa
     8941 aatgcaaatt tatttgttcc cgaaaagaag attggttgtt caagtgaaga agcaattgag
     9001 caaatgttta gaagtaaggc agtggatttt tacgtatgga ataaattttt taaaagggaa
     9061 ttattcaatc atatagtttt tccagaaaat aaattatatg aggatatggt tcctatgtat
     9121 gaagctttta gagcggctag taaaattgat attttgccat tttcaggtat tttttattat
     9181 caaaataatg atagcatcgt ttatcagtct tttaattcaa aacagtatga taatattttg
     9241 caaagaaaaa tattagttga tgaagtaaag aaagattatc ctcgacttat taatttggcc
     9301 aatgctcgat tagtagacgg atacctttca acagggttta aaattactgg caagaatcaa
     9361 aatcagaaag aaaagcaaaa atattataat cagtctaaaa gagaaataaa aagtatatat
     9421 tcaaaaataa gaagtaacaa tgagatatca attgctaaaa aattagcttt atttttatac
     9481 ttaattaatc ctaaaactta ttttatttta tataaaaaag ttctaaaaaa ataaagtttt
     9541 tttcgatatg ttatttttta tgtttattaa cttcggaaga ctaaaattgt tactggtaat
     9601 gatacatttg taaaaataaa aaagatgatt tttaggaagt gaagagagtg cataaaatta
     9661 gtgttatagt acccgcatat aacgtagaag actatttaga aaactgctta gaatctattt
     9721 ccaaccagac acattcaaat attgaaataa tagtagtcaa tgatggttcc ccggatcgaa
     9781 gtggggaaat tgcagaagaa tataaaaaga aagatatgcg attaaaagtg atacatcaag
     9841 tgaatggtgg tttaagtgcg gctaggaatt ctggtcttga ttctgcaaca ggagattttg
     9901 tgtgctttat tgacagcgat gattggatag aaccagacta tcttgagtta ctatattttg
     9961 gcacgcaaac ttatggagca gatatctcgg tgctaaaaat aaaaaagatt tcaagtttag
    10021 aacaactaaa agaacataca attgctgaga gaaaatggga aatatttgat agagaacaag
    10081 caatgaaaaa cttatttata aataataaaa ttggctattc tgcaaataat aaattattca
    10141 atagaaattt atttgataat ataagattcc cactgggtaa attaatggaa gataaggcta
    10201 ctacttattt gctgatcgat cattccgaaa aagttgctgt caattcatct gctaaatatc
    10261 actattacca aagccctaaa agtatactga gaggaagctt caatcctaga atttttgatt
    10321 cttttgaagt ccatgaagat atactaatat tcatagataa gttttatccg gaattatcaa
    10381 ttaatgttag aactcgctat gtctatgaag caataaggat gatgatgaca atgattaaaa
    10441 ataactatac caataaagaa gattatttac gttctataaa tattttacaa cgctttgaaa
    10501 aagaagtgaa aattgatccc gacttttcac caaaattaaa aaaattagta aagtttttat
    10561 taataaataa aaacttgcct tactatatgt caagtagtaa gatagcaagt aaattcttga
    10621 ttaagataga atctgtgagg taagagatga agaatataaa aaaatttttt tcaaggaata
    10681 ttttcccggt ttttattaaa atattaccta ttcaaaaaaa tttaattgta ttttcaagtt
    10741 ttggcgggcg taactattct ggaaatccaa gagttatttc agagaaaatt catgaaaaaa
    10801 aattgaatta cagaattatt tggttaatca aaaaagaagc gattataact gatttaccag
    10861 attatattaa aagaattgac tccgattctt ttttagctgc cttttattta tatacagcta
    10921 agtattggat agacgatagt cggaaaacga ttaagtatgc aaaaaggaaa aaacaacttt
    10981 attttcaaac atggcacgga acgcctttaa aaaaaattga atttgatgca aaagataagt
    11041 tacctaaacg ttacctaagc tatgcgaaaa aagatagtga gtcaattaca tatttattat
    11101 ctggtaatcg ctatagttct aagaagtatg tttcagcatt caatatcgtt ccttctaaaa
    11161 tattagaagt aggaactcct agaaacgatg aattagcctc tagtaaagag caagtgtttg
    11221 acttgaaaaa gaaacaaatc aatgtcttat ttgctccaac ttttcgaaat aatattgagg
    11281 acaacggaat tacgcaacta gaatggttag gtgtggataa cttacgtgaa ttttttaaaa
    11341 agcaacaaca agaattaaat tttatgacta aatttcatcc taatgttcat agtaaattag
    11401 caactgattc taaaagtata gaatatatga aaaaacatca aattacttta attaatgatg
    11461 ggattcctct tgaaatgctt tttgaagaga tagatgtttt aatcacagat tattcatcta
    11521 tttttttcga ttttgcgcta acaaaaaaac caattattct ttttaactat gatgaaaaag
    11581 aatatagctt agaaagaggt ttttatatac agttaagtga gttaccaatt ataagcgtac
    11641 aagatagtta tggccttgtt gatgttttta aaaaccaaca agaaaagctt ttgaaaagtt
    11701 ctcaagagtt acttgattat attggaaatt atgaaacagg agcaagtaca gaaaaggttc
    11761 ttgagttgat agagagggat ataaagtgaa aaaaacagta aaagatatat tttataatgc
    11821 gatatatcaa gtctttttga ttgttttacc cttacttact atacctatac ttagtcgtag
    11881 aattggttca actggtttag ggatttatgg gtatgttttc tcaatatcac agtttttaat
    11941 gactgtcatt gctgtgggaa tgaatccttt tcgtattcga aatatcgcta aatcaaggaa
    12001 agataaaaaa gcactatctt tacaattctg gaatatttac tttattcaat ttttaatagg
    12061 cttgagtgtt tcctttctct atatagccta tatttatttt tttgatgtaa agtataaaaa
    12121 tttatactta attcaattga tatttattgt agggctaaca ttagatattt cttggttttt
    12181 tcaaggaatt gaagaatttt cgaaagtggt tattagaaat acagttatca aattatcatc
    12241 ggtattgtta ataatcattt ttgtttcaac taaagatgat ttgtggattt acgtgttcat
    12301 tacttcaatt actaactttt taggaagttt agttttctgg ttaagtatcc acggaaaagt
    12361 aggtaaacca atctttaact ctaatgtttt taaacagttg tggaaaccag cttttattat
    12421 tttgactcct cagttgttta tgcaagtgta cacaacttta gacaaaacga tagttgggta
    12481 ttttgtggat ccaacggaat tatcttatta tgatcagtct caaaaaattg ctcgtattat
    12541 attggcagtc ttaacatcaa ttactattgt tatgttgcct aaaatatcta aagcacaggc
    12601 agaggggaaa tcagaaaata ttttagttta cacaaaaaaa tcttttgatt acacgattat
    12661 cattgcatta attcttttta gtgtggtgtt tatcaataca aaagaatttg ttccatggtt
    12721 ttttggggag aatttcaaac cgatgacaat aaacatgctt atttcttgtc taattatagt
    12781 tgttagtcca attggtggag tttttgctaa ccaatttgca attgctatag aaaaagataa
    12841 ggagtatgct tatccaatga tcattggtgc gattgtaagt attattggaa atgtagcttt
    12901 tgtgcctact tatggcgctg tagcggctac gatcgtactc gtggtagtag aattattagt
    12961 ctttttcttt agagtatttt ttgtacgaaa agaaataaaa atatcctatt tctttgggaa
    13021 gaacgttatc ttatttttta tagtcaatct tactttgact acttgggcct attactttct
    13081 tccacaatta ttctcttctt catttttaga tatggcaatt aaatcaataa tgattttcat
    13141 tatttatctt ccgtttgttt attttctttt tccagatata cgaaatgaaa taagaaagat
    13201 tactcgaaaa taaaataatg ttgtaaaaaa gtagttcttt gtcaaatcgg gctgataagg
    13261 tcatttatga acaacaaagt tagtaagagt tactagcttt taatgcatag ctgattaacc
    13321 tgttgattag aaaaatccac aagcgcatat tggtaaaact gcgaaaccca taagcaaatc
    13381 gtttcaatgt tttgatatgt gtgcgaagat tctctaattt tgttattaga atactttttc
    13441 gaaaatgcta gttaaaagcc tttttcagat atgaaaaagg ctttttgaga tagtgaagtt
    13501 tcgagggaac ttctttggtc gtttctcgaa gtagctagct gtttcttgtg ataattgcta
    13561 agcagctttt gataaactgc ttagcaattt taagttcata agagattgag ggtagacgat
    13621 caactacttc actttctgtt aaatagcgcc attgaaaatt ctgccatttc ttgaatgttg
    13681 tgtaatccaa ctcgtcatct gattttagaa gtaatttata caaaggtttc aattgtcgat
    13741 agtacttagc ttgtgaagtg tctttttgat tcaactgctt catcacacgt tttcgaacac
    13801 gatcgaaaaa cttgttcaag tgttgtccaa tgtgaaagca atcaatgatg atttgggcgt
    13861 ttggaaagga ctctttgact aatgaaaaat aaggggcatt catatttgtg acaatgtatc
    13921 gaacccccat atgagctttt cttgtaaaac gcatgaaata agcaatcaaa tctttttttc
    13981 tccggctgga caaaatgtca aagagcttcc ctgtctctct attcatacaa gaaaaggaga
    14041 actttccatg ggaaattgaa agagagcgaa actcgtcgaa tagtaaacaa tgaggtaaat
    14101 agtctagcat ctgtcgcagc attgccgata gagcagataa tgtccgttga aatgtcgtta
    14161 ctgaaagatc acaacgtttc gtaagtgtct ttaacgcttg acgctcggtc aactcttcta
    14221 atatcgtgta atgaacttgt ttcgaaataa aacatgaagg aacgacaaga tacgtatgag
    14281 cagtaaaata ttttccgcat gattggtagt ggtaatgttg ttttctaaga cgcaaatagg
    14341 tttatttatt cccactgcga tttaagataa tcattgaaat cttcttgccg tttttcacga
    14401 gctgcttcgc agtttcttta caaaatagac aatgtctaaa agtgcgaact aatgtacctt
    14461 cgattacgat ggctttcttg tccttataaa aagttttttg aaaagaatta atttcaatat
    14521 ttttatcttt aatagtgaag tgctctttga tagaacgaga aatggatata caaaatcttc
    14581 ctttcagatt gatgttttag acgactctat tttatcgaaa tttttgtatg tctgtttttt
    14641 ttacaagaaa atctaggatc gacagaaaat tctatcagtc ctagaaatta tagagccatt
    14701 tttttacgtc ttatccagat atattgttct ttcattgttt aaaaaatatt atttatcact
    14761 atttaaagaa ttaagttcgt tctttatttg agttgtagag atctcaggtg ttcttggtaa
    14821 ataaattact tgtgcatctg ttagttgttc aagttcatca aattgtccag cccaatcatc
    14881 gcccatcacg aaataatctg catggtattc ttttatatct tttacttttt gttcccaatt
    14941 ttcttcagga ataactaaat caacgtagcg aatagcttca agtaactgtt ttcttttttc
    15001 ataactaaaa taacattttt tttgtttcgc attccaattg aattcatctg tagaaagggc
    15061 aacaataaga taatctccat attgttttgc tcgtttcagt aaattgatat gcccgtagtg
    15121 aagcaaatca aaggtaccgt aagtaattac tcttttcatc tttcacctct cgaaattcat
    15181 taatatacat tgttagttta gcattttttt gttagaattg aaagaaccag tttatcctaa
    15241 aaaggataag aaatgtttat gagaaatagg tggatggagg aaaaatgttg aatgaaaaat
    15301 ttttaaaata tgtgaaaggt tggtatttta tttattttgg ttttattcta atgttacgag
    15361 caataggccc gtatttacta ttacctacta gatatgatac tttacttttt tggcctgctg
    15421 gaatagcggg agttgtcttg atcattatag atgcaattat tgcatttaga gaaaaaaatc
    15481 ttaaaaaata tgattggcta ttaattgctt ttattggcgc gatgctagta agcagtttaa
    15541 tgaatttaaa atatggatat agcgaaaata tcaagttaat catgtggcaa ttaatctttt
    15601 tctttatgat ttatcagttt ggcaaagact acagtagtaa cttttttttc aaagtattta
    15661 cttggatact ttcattggta tggtttttag ctaatttagt atcattatac tggtttgtca
    15721 ttcaatatgg cataaaaatt ccaataaaat ataagtatta cccagttcgt ttagggtggt
    15781 taggcaaccg tttatttggt gtatatacag atccaaattt tggtgcggtt atatcattaa
    15841 ttgtcatcat tattgctata tattatttgg tcacttacaa gcatttaaat cttctattta
    15901 aaatattttt tgcaattaat atttttattc aattctcgtt tattgcttta tcgggctcac
    15961 gcaccacatt agtttgttta ttaatagcaa cttttgtttt tagtttcttt acaagttata
    16021 gtagtcgttt tcttaaaact aatactttaa aaagatggct aactgcatta gtagtaggat
    16081 tgctaagtat aattgcgatg tattatgtaa cagatataac tgctcaagca tgggcacatg
    16141 ttccgcatat tacagaaata aaaaaagata tatctgattc atcaggaaca actagcagca
    16201 ataaagttga tttaaaacgc cctgacactg atggcgggga tgtttctaat ttaaggtttg
    16261 gactttggaa aagtgcaatt gaaatattca agagtaagcc gatatccggc ggttctccag
    16321 gatactatat tgaatacgca catgataaac taccatatac attaatggga aaagatactt
    16381 taactgctca taattttatt ttcttagtta tggctgctac aggaggatta ggtctattga
    16441 cattgttaac cttcttattt atccaatgct ttaaaacttt gcgttattgt ttcacacaac
    16501 caaatctagt aaaagataag cacttttttg ctattttaat gattcttaca atagcagttt
    16561 cggcattatt tatcacagaa ctcattttgg ttagtacgat aggttcattt attttttggg
    16621 gatatctggg ttctatccaa aaaactatat ctaaagaaga aggagtaact aattgaaact
    16681 atttttaggt atataaagta gaaaagaaaa ctagttatcc caacttcaac aaattttata
    16741 gtttttacaa tataaaaatg gtaaactaac tttattaagt atgagaatgc ataattggag
    16801 tgaatttgat ggatactata ttgattactg gtggagctgg ttttatcggt tcaacgctag
    16861 cgaattattt gggaaaagaa aacaaaatcg tagtagtcga cgatttatca atgggaaaaa
    16921 aagaaaactt ggatatggag aaacaagtga ctttcattga gggggatgta tcagatcctc
    16981 aattgatgga aagaatcatg aaacagtatc agtttgccta tatttttcat ttagctgctg
    17041 tagctagtgt ggcagattct gtagaacgtc ctctagaaac tcatcgtgta aattttgaca
    17101 gtgcgctttt attgcttgaa ttggtacgaa aatatcaatc tggtttaaaa cgattggtat
    17161 ttagttcatc agctgcagtt tatggcgatg aaccaacgtt gccaaaaaaa gaagaatcgg
    17221 tcatccgtcc tttaacacca tatgccatcg ataagtttgc atcagaacaa tatgttttgg
    17281 attactgtca tttatatgat gttccgacta gtgctgtccg attttttaac gtttatggtc
    17341 caaatcaaaa tcctaactct ccatatagcg gagttatttc gattctagtg gaccgataca
    17401 aaaaacaatt ggcaggagaa aaaacagaat ttactttatt tggtgatggt agccaatcta
    17461 gagacttcgt ttatatagaa gatgttatac aagcactttt acttgttgct aaggaagaaa
    17521 aagctttagg acagcaattc aatgtcggta caggaaaaag cactacgcta cttgaattga
    17581 ttcattcaat tgatcaaatc ttaggaacag agctagcgct taaatatgaa gcggagcgtt
    17641 caggggatat tcgtgattct ttagcggata tttcaaagat ccgttcttta ggttatcaac
    17701 ccaaatttga tatattgaac ggaatggaac gttatttaaa gacagaaatc gattgaaaca
    17761 aaaaaccagc atcctgctgg tttttttgtc tcgacttagc tttttaaatt aacaccttcc
    17821 atcagttcgt aaatctgatt tcttaaatca gtggccggca atttgatgtt tttatcagat
    17881 tggatattac tttccatgac gattgcatct tttgtgtctt ttttcatcgc cgctactgca
    17941 tggaatcgat tgatatttcc ctgagcaagg atataatcat ttccactgta aacaccgccg
    18001 ctgtaaggat gtttatcacc attattttgc cacaatgaga aaagtagttc ttttggtata
    18061 atatcgccag acagaacact ggtaaagaat ttgttcaagt cataaacaga catacttata
    18121 tttcctgtgc ctaattcatt tcggatgtct gtttcaggct cgaccaatgc ttttgaataa
    18181 tcgtcagttg cagacatttg gtaagataca gcatgctgag gggaattttt tacattttca
    18241 taaaaatctg tatgttctag atggagtggc cgaatgattt ctttttttaa aagagtttca
    18301 taaggctgat tcattagttt gatcaatacc cctgtaagca atgtataatt taatggttca
    18361 taatgatacg tatcataacc agtaaatttc aaatgatgaa gggcaaactg aatgatctca
    18421 ctatctgaca taggtttttt tggagaagcg gttcgatgaa gaccagagcg catgtaaagc
    18481 atatttttga ttgagatttg ctgacttcca ggaatctgag gataaaattc agatagcttt
    18541 gtgtcaaaag ttatcttgcc agattgaatt tgttttagga taagtaaagc agtcatcgct
    18601 ttttggatcg aaccgatttg atagtaaaga gaaggagaat tcgattcttt tatgcgtaga
    18661 tttgcgtacc catatccttt ttctaatatc actcgattat ttttgcgtac ataaatcgaa
    18721 ccgatataat ctgatttttg cagtaattga tcaattttgc tggcaagcat ctgctcatta
    18781 gatgttaaat tggaagcaag ttgtgcgggt tccgttttat aaatttgtgg ttgaggttgt
    18841 tttgttaatt gccatcctgt aatgatgcct gttgaaaaga agctgacagc cagcaacaaa
    18901 actgcgagaa aagaaagaga gatctgctgt tttttttgat gttttccttt tgccatatta
    18961 aatcctttct atccattttt aactattttt cattgtaatc ctcaaaaaga gcatttgcca
    19021 cttacaattt tgacataata tttggtaaaa taattcacgg cagtagaaat ttcatgtata
    19081 ataaacagag ttacgaagat gatagtaagg aggagttgtt ttgtcaggaa acaaacaaca
    19141 cccaagcaaa gacaaaaaag aagccagtaa ggcagctaca gcagatataa tcgacaagcg
    19201 gagaaatctg cgtaaaaaag aagataagat cgtgaggaag atcattttag tgatcgctct
    19261 tacattattg attattggcg gatttctcgg gtttaccgta tatcgttatg tagatagcgg
    19321 gttgaagcct ttagataaat cggacgacca attagtccaa gtggaaatcc caaacggctc
    19381 ttctaataag cagattggcg aaattttgga gaaagacaat attattaaaa gtgggattgt
    19441 tttcaattat tatacaaaat ttaaaaactt aactggcttt caagctggtt attatcagtt
    19501 agcgcctaat atgactcttg atgagattgg caagcagttg caagaaggcg gcacatctga
    19561 gccaacaaaa gttgcagatg ggaaaattgc cattccagaa ggctacgaca tcgatcagat
    19621 tgctgagcgg gtagcgaagg taacaggtaa agacaagaaa gaatttttag atttagtaaa
    19681 tgatgagact ttctttaacg agttgtatca aaaattccca gaattgttgg aaagtgcctc
    19741 taaagctgag aacacaaaat accgtttgga aggctactta ttcccagcta cttatgatta
    19801 ctacacgagt acttcgctta aggatcttgt gattgagatg gtgaacaaga caaatacggt
    19861 catgcaaaac tattattcag ctatcaaaca gaaaaatttg actgtacaag aagttttaac
    19921 tttggcttct ctagttgaaa aagaaggcgt aaaagaaaat gatcgtaaaa atattgcaca
    19981 agtcttcttt aaccgaatca aggcaaatat gccacttcag tcagatatct ctgttttata
    20041 tgcattagga gaacataaag aattggtgac gtatgaagat acagctgtag attcaccata
    20101 caatttgtat accaataccg gctatgggcc tggtcctttt gacaatccaa gcgaaaaggc
    20161 aatcaaggca gtattagaac cagcagaaaa tgactattat tatttcgtag cagatacgag
    20221 cactggaaat gtttatttcg ctaaaacgta tgaagaacat atggaactag tgcaaaaata
    20281 tgtaaataat tcttagaaaa acaggggcta gtcattgatc atttttagaa aaacatttac
    20341 attttaagaa aagcgtggta atcttttttg gactatattc ctcaaaagat taccttttgc
    20401 ggtattttat cgaaaaaagg agacgttaca catggtagag aaagtttatc caatgactct
    20461 agaaggtaaa gaaaaattag aaaaagaatt agaagaacta aaaacagtta aacgcggaga
    20521 aatcattgaa cgaatcaaaa tcgctcgcgg tttcggagat ctttccgaaa actccgaata
    20581 cgaatcagct aaagatgaac aagcatttgt tgaaggaaga atcacaacaa ttgaaaatat
    20641 gatccgtttt gcacaaatca tcgataatga cggcgtagat gcagatgagg tatcgatcgg
    20701 taaaacagtt acgttcatcg agttgcctga tggagatgaa gaagagtaca cgattgtcgg
    20761 aagtgctgaa gcagacccgt tttctggaaa gatttccaat gattctccta tcgcgcaagc
    20821 attgattggc aaacgtaaaa atgatgaagt aaccattgct acaccaggtg gcgatatgtt
    20881 agttaaaatc gtcaaagtag aacaagcgta gtctgactga aagatctaga gagttcatct
    20941 tactgatgac gcactctaga tcttttctct ttgagtattg caggtttcac tgaacgatct
    21001 tacatctaaa gtcctatagc caacagtcaa aaaaataggt atgatgaaat gagaatgtgt
    21061 caggagggag aaaaagcgat gaatagttct tggcgttttt ttgtggtagt cgaagcactg
    21121 ctgctgttat ttgctgtttg gcaaatcgta aacaatactg gacttttgct tttagtaata
    21181 ttcggtattt tcaatatata tctagctatg cgcaaatatc ctcgtactaa atttcaaaat
    21241 tttcaattga tcttagggag cttggtcatt ttttttagct tagtcaacag tcctgcttta
    21301 tggatgatgg ttgtattggc cgttttattt atcgggctga aaggtgtaga gatttcagga
    21361 atagatttga caaaaaatgc tttttggcga aaaaaacaga tcatgatggt ccaaacagag
    21421 caattaaaaa cacataataa tgagcgtaaa agacagcaat tatttggtaa ccaacgtatt
    21481 ggaaacgacg tctatgaatg ggatgatatc aacattgcca tcatttctgg tgatactatc
    21541 atcgatttgg gtaatacact tttgccaaaa gatgataata ttgtgattgt aagaaaaggg
    21601 ataggcagaa cacggatctt agtacccctg ggagtggcta ttcgtttaga gcatgcgaca
    21661 ttagtaggaa atgtcttgtt tgaagaagag cagttttcct taaaaaacga acagatcaaa
    21721 atctgcagca gtgattatga tgaaaatcct cgcagattga agatcattac caataccttg
    21781 gttggagatg tcgaggtgat acgaatatga tgggaagaat atccaaagca atgctagctg
    21841 tttattcggg gattgcggct ttccttatta ttctattttc acttttcact tatttttatg
    21901 ccagtaacca aagtcattgg tggggagaat tgctgcgtgc gcgtttatta tacgttccgc
    21961 ttatcttcca tttgatggca atttcattag gtgtaggatt gatcgtattt ctattgttat
    22021 cacttattca aaaggcaaaa tacgggaaaa tcgaagaaaa gcttcgtgca ctttcttctg
    22081 gcaattatga atccaaactt ttgcatcagc cgatcccaag cgcgtcggat gatctgtaca
    22141 tcaaagatat tgatatggaa attgctaaaa taaaagaaaa aatgatcgaa gtatccagtg
    22201 aattacagat tatgactagc cgtcctcaat atgtagatgg acaaaccaaa gaagagattt
    22261 tagaattaga aaggcatcga ttagctcgtg agcttcatga ttcagtttca cagcaattat
    22321 ttgcagcgat gatgatgatg tcggcattga cggagcaagc agaaaaaagt gagacaccag
    22381 aaatgttccg taagcagttg aaaatggtgg cagagatcat caacgcttcc cagtctgaga
    22441 tgcgtgcgct gcttcttcat ctgcgtccag tcaatttaga agaaaaaagt ttaaaacaag
    22501 gcattgagca gttattgaag gaattgcaga ataaaatcca gatttcgcta aaatgggatg
    22561 tagaagatgt aaaattaact agttccattg aggatcactt gttccgaatc gttcaagaat
    22621 tgttatcaaa cacattaaga catgctaagg ctaatgaatt agaagtctat ttgcataaaa
    22681 tagataacaa tcttttatta cgtatcattg acgacgggac aggatttaat atgaatgaaa
    22741 ccaaaacggg aagttatgga ttgaacaata tcaaagaacg agtagctggt attggtggaa
    22801 cagtaaaaat cattagtttt aaagggcaag gtacgagcgt ggagatcaag gtccctttga
    22861 tgaaggaggc atagataaag atgataaaag ttttattagt agatgaccat gaaatggtac
    22921 gcttaggcgt ctcttcttat ttatctatcc aagaagacat tgaagtgatt ggagaagctg
    22981 aaaatggacg tcaaggctat gaaaaggcga tggcacttcg accagatgtc attttaatgg
    23041 atctagtcat ggaagaaatg gatggcatcg aatcaacaaa agcaatctta aaagattgtc
    23101 caaaagccaa aatcattatc gtcacaagtt ttattgatga tgaaaaagtt tatcctgcaa
    23161 ttgaagcagg agcagcagga tatttgttga agacgtctac cgcacatgaa atagctgatg
    23221 ctataagggc tacgcaacgc ggcgagcgag tattggaacc tgaagtgaca acaaaaatga
    23281 tggaaaaaat gagtcggcga aatgatccag tattgcatga agaattaacg aatcgagaaa
    23341 atgaaatttt gatgctgatt tctgaaggga aaagtaatca ggaaatcgcg gatgaattgt
    23401 ttatcacact gaaaacagtt aaaacgcatg tgtcgaatat attagctaag ttagaggtag
    23461 aagaccgaac tcaagcagct atctatgcgt ttaaacatgg tttggtgaaa taaaaacgat
    23521 gataaagagg ataatcaaaa gctgagaaaa taactattat ttttcttggc ttttttacaa
    23581 taatgatgct tgcttttgtt tttggaaagg ctttgttata ctgagaaaag aagatactaa
    23641 aggaggaaaa tggtgaaaca aaattttgca attgtaggcc ttggacgatt tggcggaagc
    23701 atctgccgta cactgatcga agctggacaa gaagtactgg ctattgacag taatgaagat
    23761 cgagtcaatg aatatatgaa tatagccact catgctgttg tggcaaatgc acaggatgag
    23821 atgacacttc gttcattagg tataagaaat ttcgatcatg taattgtcgc tattggagaa
    23881 gatatccaag ccagtatctt agttactctg atggtaaagg aaatgggcgt accgaatatt
    23941 ttagcaaaag cccaaaatga gtaccatgcc cgtgtactag agaagatcgg agcagatcgt
    24001 gtcgtacacc ctgagcgaga catggggata cggattgcac ataacttagt atcaaagaat
    24061 attctagact acttagagct gtcagataaa tattctttag cagaaatccg agtctccaat
    24121 cctaaatttt tcaacaaaac attagctgaa ttaaatttca ggcaacgttt tgacttaaca
    24181 gtagtcgcca tacgaagagt agatcaaacg gttatcgctt ctcctgcagc agatgaatat
    24241 gttcgcgaaa atgataatct actggtgatt ggagagacag gcgatgtgga tattctagac
    24301 gataagatga atcaatagaa agaaaagaga aagtaggttg ttgggatgga attaacggta
    24361 acgccaaaag cacaaaagtg gtttgactca gaaattgatc tgccagaaac ttacggaata
    24421 cgtttttttg gaaaagtcta cggcaaaaca gaggttcatg atggtttttc gattggaatg
    24481 tcagttgaac agccagaaca tcctgtaaag aaagtggaaa tggatgggac actttttttt
    24541 atcgaggaag cagatgaatg gttctttaaa ggatatgatt tacttgttga ttatgatccc
    24601 aacctggagg agccaaccta tcactttaaa aaacaataaa aaaagagagg gtgacacaag
    24661 tttt
//
LOCUS       DACELF010000041        23648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.043, whole genome shotgun sequence.
ACCESSION   DACELF010000041 DACELF010000000
VERSION     DACELF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.043"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            248..523
                     /locus_tag="IW687_001521"
     CDS             248..523
                     /locus_tag="IW687_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950787.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="IW687_001522"
     CDS             536..2614
                     /locus_tag="IW687_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0950788.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..4660
                     /locus_tag="IW687_001523"
     CDS             2774..4660
                     /locus_tag="IW687_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0950789.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4672..5619
                     /locus_tag="IW687_001524"
     CDS             4672..5619
                     /locus_tag="IW687_001524"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0950790.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5639..6166
                     /locus_tag="IW687_001525"
     CDS             5639..6166
                     /locus_tag="IW687_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0950791.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6229..6882)
                     /locus_tag="IW687_001526"
     CDS             complement(6229..6882)
                     /locus_tag="IW687_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0950792.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7014..7856
                     /locus_tag="IW687_001527"
     CDS             7014..7856
                     /locus_tag="IW687_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0950793.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8014..8910
                     /locus_tag="IW687_001528"
     CDS             8014..8910
                     /locus_tag="IW687_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0950794.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8913..9626
                     /locus_tag="IW687_001529"
     CDS             8913..9626
                     /locus_tag="IW687_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0950795.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9646..10164
                     /gene="msrA"
                     /locus_tag="IW687_001530"
     CDS             9646..10164
                     /gene="msrA"
                     /locus_tag="IW687_001530"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0950796.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10161..10388
                     /locus_tag="IW687_001531"
     CDS             10161..10388
                     /locus_tag="IW687_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0950797.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10540..11091
                     /gene="lepB"
                     /locus_tag="IW687_001532"
     CDS             10540..11091
                     /gene="lepB"
                     /locus_tag="IW687_001532"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0950798.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11174..11716)
                     /locus_tag="IW687_001533"
     CDS             complement(11174..11716)
                     /locus_tag="IW687_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0950799.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11953..12351)
                     /locus_tag="IW687_001534"
     CDS             complement(11953..12351)
                     /locus_tag="IW687_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0950800.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12611..13471
                     /gene="ylqF"
                     /locus_tag="IW687_001535"
     CDS             12611..13471
                     /gene="ylqF"
                     /locus_tag="IW687_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0950801.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13464..14231
                     /locus_tag="IW687_001536"
     CDS             13464..14231
                     /locus_tag="IW687_001536"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0950802.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14287..15144
                     /gene="dprA"
                     /locus_tag="IW687_001537"
     CDS             14287..15144
                     /gene="dprA"
                     /locus_tag="IW687_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0950803.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15266..17344
                     /gene="topA"
                     /locus_tag="IW687_001538"
     CDS             15266..17344
                     /gene="topA"
                     /locus_tag="IW687_001538"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0950804.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17313..18689
                     /gene="trmFO"
                     /locus_tag="IW687_001539"
     CDS             17313..18689
                     /gene="trmFO"
                     /locus_tag="IW687_001539"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0950805.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18887..19792
                     /gene="xerC"
                     /locus_tag="IW687_001540"
     CDS             18887..19792
                     /gene="xerC"
                     /locus_tag="IW687_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0950806.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19829..20377
                     /gene="hslV"
                     /locus_tag="IW687_001541"
     CDS             19829..20377
                     /gene="hslV"
                     /locus_tag="IW687_001541"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0950807.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20391..21791
                     /gene="hslU"
                     /locus_tag="IW687_001542"
     CDS             20391..21791
                     /gene="hslU"
                     /locus_tag="IW687_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0950808.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21809..22600
                     /gene="codY"
                     /locus_tag="IW687_001543"
     CDS             21809..22600
                     /gene="codY"
                     /locus_tag="IW687_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0950809.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22682..23557
                     /locus_tag="IW687_001544"
     CDS             22682..23557
                     /locus_tag="IW687_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0950810.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4078 c         4981 g         6397 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     4681 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     4741 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     4801 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     4861 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     4921 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     4981 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     5041 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     5101 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     5161 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     5221 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     5281 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     5341 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     5401 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     5461 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     5521 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     5581 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     5641 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     5701 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
     5761 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
     5821 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
     5881 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
     5941 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
     6001 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
     6061 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
     6121 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
     6181 gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa
     6241 taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg
     6301 catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc
     6361 tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac
     6421 gacacagagc caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg
     6481 tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac
     6541 aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc
     6601 tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt
     6661 aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata
     6721 gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac
     6781 cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc
     6841 atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact
     6901 aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac
     6961 atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa
     7021 atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag
     7081 gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc
     7141 aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa
     7201 gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg
     7261 aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag
     7321 ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat
     7381 tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa
     7441 tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac
     7501 tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt
     7561 tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc
     7621 gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc
     7681 caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca
     7741 aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg
     7801 ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga
     7861 aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat
     7921 taggaaaata attgttttct tttttcataa agactacctt tttgataaaa tttttgataa
     7981 cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc
     8041 ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata
     8101 ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa
     8161 gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc
     8221 aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc
     8281 gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga
     8341 gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact
     8401 gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat
     8461 tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt
     8521 cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg
     8581 aactttcctg agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa
     8641 acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt gctttatcaa
     8701 ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac tagcacgaca
     8761 gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt ccatccaaat
     8821 aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga gacacaaaac
     8881 caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac gtatcttcaa
     8941 acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt ggggtagtac
     9001 ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca gaactcccag
     9061 aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat tcaaataaag
     9121 aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat agtgatattg
     9181 aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa gtactaggtt
     9241 ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga aatgtccaac
     9301 taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat gtgatgaata
     9361 tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat gatctgacta
     9421 tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa gcagataaaa
     9481 tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat acctctgatg
     9541 cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc aagacaaaag
     9601 aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga acgagcaata
     9661 tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc tggtatccat
     9721 acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga acaagtaaaa
     9781 agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac attgatttca
     9841 tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc tttcggacaa
     9901 ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga agaacagcgg
     9961 atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga acagccaatc
    10021 gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca tcaagagtat
    10081 tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc ctttatcgaa
    10141 gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg acactgcgag
    10201 gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa gacatccaat
    10261 ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg aatgtcgact
    10321 atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa aataatcatg
    10381 acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca atagcctaat
    10441 tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt ttcttataat
    10501 aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa acaacgctat
    10561 attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct tgcgttcatg
    10621 ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga aaacgtgttg
    10681 aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt tgatattgtt
    10741 gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct tccaggagaa
    10801 aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa ggaaccttat
    10861 ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga ctttacacta
    10921 caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact tggagataat
    10981 cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga tattctaggt
    11041 aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg agaaacaata
    11101 aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca gtttactacc
    11161 ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa gttttgataa
    11221 atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg aatctaccta
    11281 gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa ttgataaaaa
    11341 ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg tttatctttt
    11401 tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata agaaaagcag
    11461 cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac atttcaagca
    11521 aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa aatccgaatg
    11581 tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca agggctaata
    11641 aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa acgtcttttg
    11701 caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg ataccataaa
    11761 atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa caattaaaaa
    11821 aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat aaaaaaaata
    11881 gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa aaacgtcaga
    11941 ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt ctagatcttg
    12001 gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt ggaaagtacc
    12061 accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt caacactaaa
    12121 cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt ttataaattt
    12181 tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata ccattgaacc
    12241 agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat aaaaagcaac
    12301 ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca tttgtttcgt
    12361 ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca aaaatttaga
    12421 ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga tgtgatagaa
    12481 tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat aaaaaagtga
    12541 atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta tgaaaggaat
    12601 ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc gacgagaagt
    12661 ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac gattaccttt
    12721 atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc ttgttttatt
    12781 gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat attttcgtaa
    12841 acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta aaggtctagt
    12901 acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa
    12961 gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat ctacgttgat
    13021 gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg taacaaaagg
    13081 tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag ggattttatg
    13141 gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga
    13201 ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg cccgatatta
    13261 ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa aattggcacc
    13321 ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg atcgtggcag
    13381 cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac tggatcgttg
    13441 cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa gaacgattga
    13501 aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat ccacgcaaag
    13561 gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca ttaattaagg
    13621 aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt caccaatgga
    13681 ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg gcagctgcag
    13741 ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa ttatcagaga
    13801 aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc gggatcggga
    13861 tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa ttagcgatga
    13921 tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat gcgatgacct
    13981 tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg
    14041 ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc
    14101 tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa cacttagaag
    14161 gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat
    14221 atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac gaaaggcggt
    14281 tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa aggaatcagt
    14341 agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa agaaatcgga
    14401 tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt tcgcaaaagt
    14461 tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt tatcacttgc
    14521 tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat tgtactgttt
    14581 tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg tggaagacac
    14641 acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat agaagctgga
    14701 tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca agcagcaatc
    14761 tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt ctatcctgct
    14821 gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag tgaatatcct
    14881 cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat cgcagctttg
    14941 tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat aacagcacag
    15001 caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt aacagaacat
    15061 tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc gggtgaagat
    15121 atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt gacaaacgtt
    15181 ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag taggaacacg
    15241 acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg tagaatcacc
    15301 tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa tggccagtgt
    15361 tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa acaattatga
    15421 accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga aagccgcagc
    15481 aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag aagcgattgc
    15541 ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa
    15601 tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga
    15661 tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt attcgatcag
    15721 tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc
    15781 tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag aagaatattg
    15841 gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt tctggggaat
    15901 cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca ctgctagaat
    15961 tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc
    16021 gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa actttcgaac
    16081 aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg gtaaacaagg
    16141 aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg atacagcaaa
    16201 agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta gacatgatca
    16261 ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc gtccaacgag
    16321 tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat tgaaacttta
    16381 tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt tagatacaat
    16441 gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa aaatcaaatt
    16501 caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag agaaagaaaa
    16561 tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg aaccaaaaca
    16621 acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg cactagaaga
    16681 aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc aaagacgcta
    16741 ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa ttgtcaattc
    16801 attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag aaatggaagg
    16861 agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg atcgtttcta
    16921 taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa
    16981 agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga taaaattagg
    17041 gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat
    17101 cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa
    17161 atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg aattcacttc
    17221 ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa
    17281 agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag acatccaaaa
    17341 ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca gggcttgccg
    17401 gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta tatgaaatgc
    17461 gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta gtatgttcca
    17521 attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc
    17581 gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct ggtggtgcct
    17641 tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa gagcatccat
    17701 tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc gtagtagcaa
    17761 caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat ggttcagatg
    17821 gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc gatatggata
    17881 aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac tgtccgatga
    17941 cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg gcagaactta
    18001 aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta atggcacagc
    18061 gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa gatcctaaaa
    18121 ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca gcttctttat
    18181 acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt gttttccgga
    18241 tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct
    18301 ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt
    18361 tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg agcggactac
    18421 ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag tttccacaag
    18481 aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa catttccaac
    18541 cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga gacaaaaaag
    18601 caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata aaaacagaat
    18661 tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg agacggatag
    18721 tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg aggaattgcg
    18781 ccaattgtga acaaacttag aaatattggt taaattctga caatttattt gtctttttat
    18841 aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga cagaagccac
    18901 gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa
    18961 agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat ctggagacgc
    19021 tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat tagctgatca
    19081 tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac gttcgtttta
    19141 tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg ttcatctcaa
    19201 aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag cactttttga
    19261 cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg aagtccttta
    19321 cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca ttgattttga
    19381 ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc ctctaggttc
    19441 ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat tgatgacaaa
    19501 atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac
    19561 tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata gtgatatcca
    19621 tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg cagatttgcg
    19681 gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct atgcccatgt
    19741 aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt aaaaataagg
    19801 aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt caaccactat
    19861 ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag taacaatggg
    19921 tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca atggagaagt
    19981 tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg
    20041 caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag ctcaagaatg
    20101 gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga atgataaaga
    20161 aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta tcttagctat
    20221 cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact atgctcaaaa
    20281 cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag atatctgtat
    20341 ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat atgtatgaat
    20401 taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta ggacagcacg
    20461 cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg cagttagatg
    20521 aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct acaggggtcg
    20581 gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg
    20641 aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca atggttcggg
    20701 atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt gtatacacaa
    20761 gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag
    20821 aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat gctagtcagc
    20881 aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat cgccgtacag
    20941 tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt gaagttgaag
    21001 accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg atcgacctaa
    21061 atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta acagtcaaag
    21121 aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat gctgatatcc
    21181 atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat gaaatcgata
    21241 agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc caaagagaca
    21301 tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc caaacagatc
    21361 acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat ctaattccag
    21421 aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct gaagattttg
    21481 tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg ttgatcggta
    21541 cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct caaatcgctt
    21601 tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc attttggaaa
    21661 aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa ataacgatca
    21721 cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat ttaagtcgct
    21781 atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa cccgcaagat
    21841 aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc cttacgataa
    21901 aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag gaagtgacgg
    21961 agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt
    22021 tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga tgaaaactga
    22081 agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa gagagaaata
    22141 tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt tgggtacgat
    22201 tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag cagaatatgg
    22261 agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg aagcagatgt
    22321 acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg aattgaaggc
    22381 tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg cttctaatat
    22441 tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa aattggaatc
    22501 agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa aagtattgaa
    22561 ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg actggggcag
    22621 atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg gaggaatggc
    22681 aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac acggtgcgga
    22741 attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag gtgatccagc
    22801 gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa aagatgatca
    22861 gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc gggatatgga
    22921 ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga gtacgccgga
    22981 gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat taggaggaga
    23041 cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt tcttttctat
    23101 cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg aagatttcta
    23161 tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc cattcatcga
    23221 tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc gtgaactttt
    23281 tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga
    23341 aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag ggatctggtc
    23401 tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac
    23461 ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag gtgcccatga
    23521 aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg agaagttagc
    23581 agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg
    23641 acatcgat
//
LOCUS       DACELF010000042        23525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.044, whole genome shotgun sequence.
ACCESSION   DACELF010000042 DACELF010000000
VERSION     DACELF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.044"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(196..501)
                     /locus_tag="IW687_001545"
     CDS             complement(196..501)
                     /locus_tag="IW687_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0950811.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(526..957)
                     /gene="ruvX"
                     /locus_tag="IW687_001546"
     CDS             complement(526..957)
                     /gene="ruvX"
                     /locus_tag="IW687_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0950812.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(954..1220)
                     /locus_tag="IW687_001547"
     CDS             complement(954..1220)
                     /locus_tag="IW687_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0950813.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1475..2200)
                     /locus_tag="IW687_001548"
     CDS             complement(1475..2200)
                     /locus_tag="IW687_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0950814.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2561..2631)
                     /locus_tag="IW687_001549"
     tRNA            complement(2561..2631)
                     /locus_tag="IW687_001549"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2597..2599),aa:Gly,seq:ccc)
     gene            2748..3500
                     /locus_tag="IW687_001550"
     CDS             2748..3500
                     /locus_tag="IW687_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0950815.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3516..3995
                     /locus_tag="IW687_001551"
     CDS             3516..3995
                     /locus_tag="IW687_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0950816.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4246..5826)
                     /locus_tag="IW687_001552"
     CDS             complement(4246..5826)
                     /locus_tag="IW687_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0950817.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5819..6562)
                     /locus_tag="IW687_001553"
     CDS             complement(5819..6562)
                     /locus_tag="IW687_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0950818.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6738..7514)
                     /locus_tag="IW687_001554"
     CDS             complement(6738..7514)
                     /locus_tag="IW687_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0950819.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7762..8598)
                     /locus_tag="IW687_001555"
     CDS             complement(7762..8598)
                     /locus_tag="IW687_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0950820.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8609..9538)
                     /locus_tag="IW687_001556"
     CDS             complement(8609..9538)
                     /locus_tag="IW687_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0950821.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9730..10083
                     /locus_tag="IW687_001557"
     CDS             9730..10083
                     /locus_tag="IW687_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0950822.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10157..10813)
                     /locus_tag="IW687_001558"
     CDS             complement(10157..10813)
                     /locus_tag="IW687_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0950823.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11039..12973
                     /locus_tag="IW687_001559"
     CDS             11039..12973
                     /locus_tag="IW687_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0950824.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13059..14282)
                     /locus_tag="IW687_001560"
     CDS             complement(13059..14282)
                     /locus_tag="IW687_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0950825.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14276..16174)
                     /locus_tag="IW687_001561"
     CDS             complement(14276..16174)
                     /locus_tag="IW687_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0950826.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16168..17286)
                     /locus_tag="IW687_001562"
     CDS             complement(16168..17286)
                     /locus_tag="IW687_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0950827.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17369..18202)
                     /locus_tag="IW687_001563"
     CDS             complement(17369..18202)
                     /locus_tag="IW687_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0950828.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18195..19112)
                     /locus_tag="IW687_001564"
     CDS             complement(18195..19112)
                     /locus_tag="IW687_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950829.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19339..20463)
                     /locus_tag="IW687_001565"
     CDS             complement(19339..20463)
                     /locus_tag="IW687_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0950830.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20646..21554
                     /locus_tag="IW687_001566"
     CDS             20646..21554
                     /locus_tag="IW687_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950831.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21695..23308
                     /locus_tag="IW687_001567"
     CDS             21695..23308
                     /locus_tag="IW687_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0950832.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7339 a         4590 c         3947 g         7649 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
      241 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      301 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      361 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      421 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      481 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      541 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      601 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      661 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      721 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      781 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      841 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      901 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      961 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
     1021 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
     1081 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
     1141 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1201 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1261 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1321 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1381 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1441 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1501 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1561 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1621 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1681 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1741 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1801 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1861 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1921 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1981 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     2041 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     2101 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     2161 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2221 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2281 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2341 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2401 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2461 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2521 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2581 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2641 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2701 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2761 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2821 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2881 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2941 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     3001 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     3061 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     3121 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3181 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3241 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3301 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3361 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3421 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3481 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3541 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3601 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3661 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3721 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3781 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3841 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3901 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3961 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     4021 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     4081 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     4141 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4201 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4261 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4321 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4381 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4441 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4501 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4561 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4621 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4681 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4741 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4801 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4861 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4921 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4981 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     5041 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     5101 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     5161 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5221 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5281 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5341 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5401 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5461 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5521 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5581 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5641 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5701 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5761 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5821 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5881 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5941 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     6001 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     6061 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     6121 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6181 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6241 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6301 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6361 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6421 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6481 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6541 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6601 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6661 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6721 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6781 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6841 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6901 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6961 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     7021 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     7081 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     7141 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7201 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7261 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7321 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
     7381 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
     7441 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
     7501 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
     7561 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
     7621 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
     7681 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
     7741 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
     7801 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
     7861 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
     7921 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
     7981 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
     8041 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
     8101 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
     8161 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
     8221 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
     8281 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
     8341 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
     8401 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
     8461 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
     8521 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
     8581 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
     8641 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
     8701 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
     8761 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
     8821 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
     8881 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
     8941 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
     9001 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
     9061 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
     9121 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
     9181 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
     9241 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
     9301 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
     9361 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
     9421 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
     9481 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
     9541 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
     9601 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
     9661 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
     9721 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
     9781 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
     9841 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
     9901 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
     9961 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    10021 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    10081 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    10141 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    10201 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    10261 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    10321 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    10381 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    10441 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    10501 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    10561 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    10621 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    10681 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    10741 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    10801 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    10861 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    10921 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    10981 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    11041 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    11101 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    11161 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    11221 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    11281 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    11341 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    11401 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    11461 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    11521 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    11581 acaccctgac attttgattc tagatgaacc aacgaaccat ttagatatcg agacactttc
    11641 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    11701 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    11761 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    11821 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    11881 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    11941 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    12001 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    12061 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    12121 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    12181 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    12241 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    12301 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    12361 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    12421 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    12481 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    12541 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    12601 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    12661 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    12721 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    12781 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    12841 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    12901 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    12961 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    13021 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    13081 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    13141 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    13201 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    13261 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    13321 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    13381 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    13441 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    13501 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    13561 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    13621 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    13681 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    13741 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    13801 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    13861 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    13921 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    13981 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    14041 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    14101 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    14161 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    14221 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    14281 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    14341 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    14401 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    14461 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    14521 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    14581 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    14641 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    14701 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    14761 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    14821 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    14881 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    14941 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    15001 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    15061 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    15121 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    15181 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    15241 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    15301 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    15361 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    15421 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    15481 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    15541 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    15601 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    15661 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    15721 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    15781 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    15841 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    15901 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    15961 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    16021 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    16081 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    16141 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    16201 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    16261 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    16321 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    16381 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    16441 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    16501 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    16561 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    16621 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    16681 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    16741 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    16801 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    16861 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    16921 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    16981 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    17041 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    17101 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    17161 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    17221 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    17281 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    17341 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    17401 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    17461 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    17521 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    17581 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    17641 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    17701 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    17761 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    17821 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    17881 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    17941 tggctgtagc taaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    18001 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    18061 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    18121 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    18181 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    18241 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    18301 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    18361 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    18421 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    18481 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    18541 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    18601 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    18661 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    18721 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    18781 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    18841 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    18901 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    18961 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    19021 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    19081 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    19141 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    19201 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    19261 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    19321 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    19381 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    19441 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    19501 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    19561 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    19621 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    19681 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    19741 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    19801 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    19861 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    19921 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    19981 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    20041 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    20101 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    20161 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    20221 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    20281 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    20341 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    20401 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    20461 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    20521 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    20581 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    20641 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    20701 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    20761 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    20821 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    20881 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    20941 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    21001 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    21061 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    21121 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    21181 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    21241 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    21301 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    21361 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    21421 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    21481 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    21541 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    21601 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    21661 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    21721 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    21781 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    21841 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    21901 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    21961 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    22021 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    22081 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    22141 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    22201 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    22261 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    22321 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    22381 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    22441 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    22501 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    22561 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    22621 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    22681 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    22741 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    22801 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    22861 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    22921 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    22981 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    23041 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    23101 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    23161 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    23221 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    23281 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    23341 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    23401 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    23461 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    23521 aaaaa
//
LOCUS       DACELF010000043        22964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.045, whole genome shotgun sequence.
ACCESSION   DACELF010000043 DACELF010000000
VERSION     DACELF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.045"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..783
                     /locus_tag="IW687_001568"
     CDS             <1..783
                     /locus_tag="IW687_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0950833.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="IW687_001569"
     CDS             1161..3203
                     /locus_tag="IW687_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0950834.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="IW687_001570"
     CDS             3214..3834
                     /locus_tag="IW687_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0950835.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="IW687_001571"
     CDS             3846..4304
                     /locus_tag="IW687_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0950836.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="IW687_001572"
     CDS             4323..4601
                     /locus_tag="IW687_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0950837.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="IW687_001573"
     CDS             4626..5993
                     /locus_tag="IW687_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0950838.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="IW687_001574"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="IW687_001574"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ0950839.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="IW687_001575"
     CDS             7180..7671
                     /locus_tag="IW687_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950840.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="IW687_001576"
     CDS             8114..9313
                     /locus_tag="IW687_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0950841.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="IW687_001577"
     CDS             complement(9808..10566)
                     /locus_tag="IW687_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0950842.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="IW687_001578"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="IW687_001578"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0950843.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="IW687_001579"
     CDS             11666..13111
                     /locus_tag="IW687_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0950844.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="IW687_001580"
     CDS             13139..13594
                     /locus_tag="IW687_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0950845.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="IW687_001581"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="IW687_001581"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0950846.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="IW687_001582"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="IW687_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="IW687_001583"
     CDS             15403..16017
                     /locus_tag="IW687_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0950847.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            16258..16641
                     /locus_tag="IW687_001584"
     CDS             16258..16641
                     /locus_tag="IW687_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0950848.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            17004..17513
                     /locus_tag="IW687_001585"
     CDS             17004..17513
                     /locus_tag="IW687_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0950849.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17613..18263
                     /locus_tag="IW687_001586"
     CDS             17613..18263
                     /locus_tag="IW687_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950850.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18714..19652
                     /locus_tag="IW687_001587"
     CDS             18714..19652
                     /locus_tag="IW687_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0950851.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19665..20717
                     /locus_tag="IW687_001588"
     CDS             19665..20717
                     /locus_tag="IW687_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0950852.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20983..21618
                     /locus_tag="IW687_001589"
     CDS             20983..21618
                     /locus_tag="IW687_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0950853.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21809..22600
                     /gene="msrA"
                     /locus_tag="IW687_001590"
     CDS             21809..22600
                     /gene="msrA"
                     /locus_tag="IW687_001590"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0950854.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7303 a         3682 c         4842 g         7137 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgta tttattagta tatatacttt
    16201 acaaaaattt gtatatatac taattttttt gtaggatcaa tgaaatgagg ggaaaatatg
    16261 ccaaaatata aagaaatagc cttggctcta aaagagagga tcgttgaggg aaaatatcag
    16321 gaaggttcta aattacctga gcaagaaagt ctggctagag agtttaaaac aagccgtgtt
    16381 actatccgca aagctataca actccttatt gatgaaggtt tgttatatac gagacgtgga
    16441 tctggtactt ttatacgaag caatatcaga caggacaatg agaatgtcac tcaagtaaat
    16501 aatgtatttg gcacttcctc acaagaaaat acaaatgtga caagtaaaat aatccgtttt
    16561 gatgtccggt ttccgacaga ttatgaaagg cggctttctg caacctactt ccgatcccct
    16621 tcctgcaaat catgggtata aaaaataggc atattaagcg ggctaggagg agaaatattc
    16681 acatatcatt ttttcattcc gcaagctgcg tcaagttatt tggaatttgt agaacagatg
    16741 agagaggtag aagcagcact acaaactact tttcaataag taggaattat agaaatttta
    16801 ctcaaaaaat cttcattaaa aaatctgact aaataagttg agaaaacaga atatacttca
    16861 atatccagtg atttataaat atcattcact catgataccc agctccgtat cataagtgat
    16921 gcggttttta tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa
    16981 gactaaatac ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg
    17041 cgattgattt gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga
    17101 ctaaagaaga agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag
    17161 aaaataatgt gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc
    17221 tgattctctt ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa
    17281 gcaaggaaat caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta
    17341 tgaccactga tgatatttta gaagtttttg ctgaggcaga actctatcca tcaaaaggcg
    17401 aaatcggggc atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat
    17461 atatgcgtaa ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt
    17521 tcatgagaaa tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa
    17581 tattatctta atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag
    17641 ataagatgct ttttgaagag atagagaatc gatgtaggct caattttgag cttcggggaa
    17701 agatgtcttt aatacaacaa aaaaagtatt tagcgaataa atcagagttt actctgggac
    17761 atgttgaaaa attaatctcg gattggatta gtagtagaag cgaatttaca aaaataaaac
    17821 agccgattaa atttgacatg aaaaaacttt tgttaaataa gagtgagata ggtaatagag
    17881 atcaatatat tagagctaaa gggcaagaaa taattgattc attaggtgaa atgaggtcat
    17941 acaattattt gtatgtaaca catagagctg atggaatggt cattactgtg gggaaatctt
    18001 cttctaatga tattttttta gatggagatc ttttttatca actcaataca aatcacttgt
    18061 ctggcactga aaatataatt ttaaggacgg aatatggcaa tgaaatattt gcaaaatatg
    18121 atgagatttt aaaaaattat ttggactggg cttggataat tccggttgag tcaggagatg
    18181 ctaagaaact ggagagatta ttgggcgatg aactaattaa taaaaaagta ccaattttaa
    18241 attattattc ccatagacaa taaaaggcta tttttcatga taatactagt tataactgta
    18301 aaaatggaag aaaatctggg aaagaagttt ttaaataagt gtattgttcg attttaataa
    18361 ataataagca ataccatccg tctgtgttgc gcaacatcgt actacaatga atattttttc
    18421 acaaaaagaa aatcatccat cgaatgaatg gaaggtttta gtatacacga ggttaatgcc
    18481 ccctctttta taatcagagg agtgaggttt tttctatgaa tgtttctatt ttcttattgc
    18541 tagtctagtt tctcttacga tgaaattggg attctattcc agtttttatt gatttgctga
    18601 taaaataact tgaatggtgg atataaacat gataaataag agaagaataa caaaacaagg
    18661 ttgtgaaatg attctcaaat gaatataata gaaagaaaaa ggaggaagaa tcaatgaaaa
    18721 tagcaattgc aggcgctggc gcaatgggaa gtcgctttgg gttaatgctt caccaaagtg
    18781 ggaacgaagt attattaatc gatggctggg cagaacatgt ccaacaaatc aaagagcatg
    18841 gattacaagc aaattttaat ggtaaagagg tagaagcaaa actaccaatc gttcttcaat
    18901 ccgaagtaga aaaagaagat caagttgatc tgattattct atttaccaaa gcgatgcagc
    18961 tggaaaaaat gctgcaggat atccaatcat taatcaaaaa agatacagag gtcttatgtc
    19021 tattaaatgg tatcgggcat gaagatatta ttgagaaatt tgtaccaatg gaaaatatct
    19081 atattggaaa taccatgtgg acggcaggtc ttgaaggtcc tggtcaggtc aaattatttg
    19141 gaagtggttc agtagaatta caaaatttag gtgatggaaa agaagcagct gcaaaaaaat
    19201 tagcagacaa attgtctgaa tcaggattaa atgctcattt ttctgacaat attcactatt
    19261 ctatttatcg caaagcatgt gtgaacggaa caatgaacgg actatgcaca atcttagacg
    19321 ttaatatggc tgagctagga aaaacatcga ctgctcataa aatggtggcg acgattgtca
    19381 atgagtttgc caaagtagca gcagtagaga agattgaact agatgtccca gaagtcattg
    19441 cacattgtga atcttgtttt gacccggaaa caattgggtt acattatcct tcaatgtatc
    19501 aagacttgat taagaaccat cgattaacag aaatcgatta tatcaatggc gcaatttcta
    19561 gaaaagggaa gaaatatggt gttgcgacgc cttattgtga tttcttgact gagcttgtcc
    19621 atgcaaaaga agatagtttg aacgtaaaat aaaggagaga ttgaatggaa acttcacaaa
    19681 aaatatcacc gaaaattttt ctgaataaag tcctcgctgg tacagcaaca ggaatcatta
    19741 ttggattgat tcctaatgca gtattaggtg ccattttaaa atattttagt gcgtatccct
    19801 ttgcggtaac gatcgcacag ttagcagtta tttttcaact agcaacacct ttgatcattg
    19861 gtggtttgat tgccttacaa tttggatttg atccgatgag aatgatggtt gtagccggag
    19921 cttcttttgt tggttctggt gtagtcaaat tcacacctga tttgggtgaa acgggtgttt
    19981 acgttggtgc tggtacaggc gatattatta atacgatgct gacagcatcg attgctgtcg
    20041 ggctgatttt actgattgga aatagttttg ggtcagtgtc gattgtgtta acaccgattg
    20101 ttgtaggtgt aggtgcaggt ttacttggtt tttatttatt cccttttgta acagcaatta
    20161 cttctgcaat tggatcattg attaataact tcactacctt acaaccgctg ttaatgtcta
    20221 tcttgattgg ttgttccttt gcctttttga ttatctctcc tatttcaaca gtagcaatcg
    20281 gaatggcaat ccaactagat ggtgtttctg ctggtgcggc tgcgatgggc gtagctgcaa
    20341 ccacttttgt tttagtagtg aactcttgga aggttaataa gtcaggtgtg acaattgcga
    20401 ttgctttagg cgctatgaag atgatgatgc ctaatctatt tagaaaacct gtgattctta
    20461 ttccttgttt agttagtgca gtgattacag ctattcctgt cgctctattt tctatttcag
    20521 gtacgcctgc ttctgctggt ttcggcgtgg tcggacttgt tggtccgttg gcttctttag
    20581 atgcagggct aaatggcttt cttgttgttc tttgttggtt agtcatccct gtttttgcgg
    20641 cactaattac tcaattttta tgcgagaaag tatttaaact gtatgatcga gaagaagtct
    20701 tcaaattttt aggctagtag attttactaa gaaattttca atcaatggat gcgtaagtga
    20761 gatgtcaaca acatctgact tacgtttttt tgcctttttg agtaggagat tgatgctgac
    20821 aactgaactc ttcagtgcca ttcgattttt taaaatgtag aagaaactca cctttatgtt
    20881 attgattgtt caaaaggcaa ctagaataaa aatatgtgtt aaaatgagaa aaaatttgat
    20941 tgttggatgt atcgaataat cactaaaaag taggagaaga aaatgatttt tttgcaaaaa
    21001 tatggttttt attttttatt attaggtgtg attagtgatt tatcgacacc gtatatcctt
    21061 ggcctttttt atcccaaact gaatcaaatg actaccgtaa ttagtgtgtt tggtgacgta
    21121 gatagtccag tcagacgtgc tttcttggtc tggtcagttg tatctggact tttttttgtt
    21181 ctttcgttgc ctgcgttgta tcacctattt attggaacat caaaaacact tgcaatactt
    21241 gctgttgcaa cagttggttt atatgggata ggtgattgta tttttactgg gttattcagt
    21301 atcaatacga atgaaagctc ttggaatcta tcgacttgga tccataatat aggttctggg
    21361 ctaggctatg caggctttct acttttccca ttgttacttg ttctgcttta tcgtcaaagt
    21421 ggtcaaggta cgcttagcca tttttatctt gttttaacag ttattagttt actaattgca
    21481 ggtctttacg gattagcacg gattccgtcg attagtcagt ttgccttctt taagcaattg
    21541 ggcttctttc aacgcttaag ttttttcttt aactatttgc cgatgatttg tttaagtttt
    21601 tttcaactac gaaagtagaa aaagcagtgt gaagctgatt aagtaaaaga atttctctat
    21661 gggtaaccat tcgtgtggat gtgttcgact aagaatagag atgaggagag cgaaaagcaa
    21721 gttagtctta acttgttatg cgttgtgcta aatttcaggt agtcattttt tgaatgaata
    21781 agaagaaaac ttgaaagagg gtgacttcat gttagatgtg gagcagatgg aaatagacct
    21841 ttataatatt attttaaatc gaaatacaag agaatgggaa agaacacaat ttgaagaggc
    21901 aaaagaagac ctagcaaaaa tgtcgataaa gcaggtgata gaaaaactag aggtcaactt
    21961 gcgtccgcta gcactacgag ataatttgac accagatgcg atggattttt atttaagaat
    22021 cacaggggag tcagcgagtg caaaggctta tgattatgcc aaacataaga taacttcacc
    22081 cttataccaa gaacgggcga tttttgccgg tggctgtttc tggtgtatgg ttgagccttt
    22141 tgaaacaaaa ccaggtgtaa tctcagtgtt atctggttat acaggtggag agaccaaaaa
    22201 accaaattac gatcaagtca gtggtggata tactgggcat gttgaagctg ttgagatcat
    22261 atttgataca cgtcaaataa cctatgaaga gctgttggag atttattggc aaatttcaga
    22321 ccctacagat gcctttggtc aatttcaaga tcgggggaaa caatatcgtt caattctatt
    22381 tgttgttaat gaaaagcaaa ggcaattggc tgaaaaatca agagaacaac tgattcaatc
    22441 taaccgattt agccgtccta tcgttacaga gattcgccaa gcaaagactt tttggccagc
    22501 tgaaaatcat catcaacaat tttatcagaa gcaacctaaa agatataaaa agatcaagca
    22561 ttcgcatcag cttcatttat tttttcagcg acatagatag aggaaggttg tggcgcaaag
    22621 cgttcagctc cgacaaaata atgagcaact gacaaaaata gcttttcata tttttggaca
    22681 gttatgaatt atttcgagga gttgctttga gaacaccgag gaaaaggttg tggcgcaaag
    22741 cattcagctt caagtcgcaa ggaaacagga ggtatgacaa gtcttttgtc acaactcctg
    22801 tttttttact agcctttatt tccatgtgaa agagaattcg aattgtagaa tcttgatttt
    22861 tctatggaat tttgtagaga atcatcttga taattaatag acacctaatc gaattccaca
    22921 ttaatgatga actaaattcc gtataaaagc gcaaacaata acta
//
LOCUS       DACELF010000044        22024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.046, whole genome shotgun sequence.
ACCESSION   DACELF010000044 DACELF010000000
VERSION     DACELF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.046"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(59..1369)
                     /locus_tag="IW687_001591"
     CDS             complement(59..1369)
                     /locus_tag="IW687_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0950855.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1381..2799)
                     /locus_tag="IW687_001592"
     CDS             complement(1381..2799)
                     /locus_tag="IW687_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0950856.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3039..4607)
                     /locus_tag="IW687_001593"
     CDS             complement(3039..4607)
                     /locus_tag="IW687_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0950857.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4611..4892)
                     /locus_tag="IW687_001594"
     CDS             complement(4611..4892)
                     /locus_tag="IW687_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0950858.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4889..5812)
                     /locus_tag="IW687_001595"
     CDS             complement(4889..5812)
                     /locus_tag="IW687_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0950859.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5809..6687)
                     /locus_tag="IW687_001596"
     CDS             complement(5809..6687)
                     /locus_tag="IW687_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0950860.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6674..8011)
                     /locus_tag="IW687_001597"
     CDS             complement(6674..8011)
                     /locus_tag="IW687_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0950861.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8014..9117)
                     /locus_tag="IW687_001598"
     CDS             complement(8014..9117)
                     /locus_tag="IW687_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0950862.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9191..10699)
                     /locus_tag="IW687_001599"
     CDS             complement(9191..10699)
                     /locus_tag="IW687_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0950863.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10665..11357)
                     /locus_tag="IW687_001600"
     CDS             complement(10665..11357)
                     /locus_tag="IW687_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0950864.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11379..12353)
                     /locus_tag="IW687_001601"
     CDS             complement(11379..12353)
                     /locus_tag="IW687_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950865.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12653..13525
                     /locus_tag="IW687_001602"
     CDS             12653..13525
                     /locus_tag="IW687_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0950866.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14107..16305)
                     /locus_tag="IW687_001603"
     CDS             complement(14107..16305)
                     /locus_tag="IW687_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0950867.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16537..17529
                     /locus_tag="IW687_001604"
     CDS             16537..17529
                     /locus_tag="IW687_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0950868.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17589..19010
                     /locus_tag="IW687_001605"
     CDS             17589..19010
                     /locus_tag="IW687_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0950869.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19003..20502
                     /locus_tag="IW687_001606"
     CDS             19003..20502
                     /locus_tag="IW687_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0950870.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20541..21935
                     /gene="uxaC"
                     /locus_tag="IW687_001607"
     CDS             20541..21935
                     /gene="uxaC"
                     /locus_tag="IW687_001607"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0950871.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6668 a         4494 c         3823 g         7039 t
ORIGIN      
        1 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
       61 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      121 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      181 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      241 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      301 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
      361 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
      421 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
      481 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaattga
      541 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
      601 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
      661 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
      721 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
      781 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
      841 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
      901 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
      961 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1021 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1081 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1141 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1201 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1261 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1321 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     1381 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     1441 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     1501 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     1561 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     1621 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     1681 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     1741 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     1801 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     1861 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     1921 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     1981 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2041 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2101 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2161 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2221 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2281 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     2341 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     2401 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     2461 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     2521 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     2581 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     2641 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     2701 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     2761 ttttactgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     2821 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     2881 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     2941 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3001 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3061 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3121 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3181 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3241 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3301 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     3361 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     3421 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     3481 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     3541 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     3601 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     3661 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     3721 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     3781 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     3841 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     3901 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     3961 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4021 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4081 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4141 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4201 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4261 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4321 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     4381 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     4441 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     4501 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     4561 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     4621 tttctcattc ttactcgata taaactgaat gccccagctg ctaaaaaagc aagtgaaata
     4681 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     4741 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     4801 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     4861 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     4921 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     4981 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     5041 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     5101 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     5161 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     5221 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     5281 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     5341 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     5401 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     5461 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     5521 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     5581 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     5641 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     5701 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     5761 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     5821 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     5881 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     5941 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     6001 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     6061 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtgtttcgat
     6121 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     6181 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     6241 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     6301 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     6361 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     6421 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     6481 atctccgccg tgagaatgca ggtaaaggac cgtcggaaac ggtccttctt tttctggata
     6541 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     6601 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     6661 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     6721 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     6781 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     6841 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     6901 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     6961 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     7021 tcgaaaaatg gatccttctg aatgctgatg tccgttcatc tatcggataa gccttttttt
     7081 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     7141 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     7201 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     7261 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     7321 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     7381 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     7441 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     7501 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     7561 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     7621 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     7681 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     7741 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     7801 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     7861 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     7921 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     7981 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     8041 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     8101 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     8161 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     8221 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     8281 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     8341 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     8401 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     8461 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     8521 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     8581 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     8641 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     8701 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     8761 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     8821 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     8881 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     8941 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     9001 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     9061 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     9121 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     9181 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     9241 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     9301 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     9361 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     9421 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     9481 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     9541 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     9601 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     9661 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     9721 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     9781 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     9841 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     9901 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     9961 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
    10021 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
    10081 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
    10141 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
    10201 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
    10261 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
    10321 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
    10381 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
    10441 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
    10501 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
    10561 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
    10621 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
    10681 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
    10741 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
    10801 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
    10861 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
    10921 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
    10981 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
    11041 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    11101 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    11161 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    11221 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    11281 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    11341 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    11401 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    11461 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    11521 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    11581 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    11641 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    11701 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    11761 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    11821 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    11881 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    11941 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    12001 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    12061 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    12121 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    12181 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    12241 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    12301 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    12361 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    12421 gtatataatc atttttatcg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    12481 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    12541 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    12601 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    12661 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    12721 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    12781 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    12841 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    12901 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    12961 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    13021 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    13081 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    13141 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    13201 atttttcaaa attatgctgc cattagcttt accgtctatt gctactatct cgctttttta
    13261 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    13321 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    13381 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    13441 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    13501 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    13561 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    13621 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    13681 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    13741 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    13801 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    13861 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    13921 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    13981 attaaacggc gtttatcttg ttgattctgc ggcaataaaa tcagccagca gcaaaacatg
    14041 aagtgctgtt ttttgctgtc ctcttttatt gcttgaatca gatcacaaga aaaacagcct
    14101 cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa
    14161 gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga
    14221 gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc
    14281 accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg
    14341 caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg
    14401 cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt
    14461 gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt
    14521 cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa
    14581 ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg
    14641 atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg
    14701 atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag
    14761 tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt
    14821 atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag
    14881 cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg
    14941 tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc
    15001 cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc
    15061 ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc
    15121 accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg
    15181 agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct
    15241 aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact
    15301 taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt
    15361 cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc
    15421 aaatctgtcc gaaaataagt aggaaaaaag accaatgagt cctaccagtg ttaaagacac
    15481 tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc
    15541 taatctgtaa atccaattat tataatctga tttcagtact atatacgttt tatctttttc
    15601 tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc
    15661 tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat
    15721 gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc
    15781 caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt
    15841 tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaac
    15901 ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact
    15961 gactaaatca taattattat catccataac aatcgtttct ataaaattta gttctttcct
    16021 gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa
    16081 atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac
    16141 gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga
    16201 ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa
    16261 aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc
    16321 cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc
    16381 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
    16441 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
    16501 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
    16561 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
    16621 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
    16681 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
    16741 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
    16801 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
    16861 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
    16921 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
    16981 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
    17041 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
    17101 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
    17161 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
    17221 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
    17281 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
    17341 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
    17401 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
    17461 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
    17521 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
    17581 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
    17641 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
    17701 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
    17761 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
    17821 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
    17881 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
    17941 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
    18001 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
    18061 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
    18121 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
    18181 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
    18241 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
    18301 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
    18361 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
    18421 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
    18481 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
    18541 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
    18601 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
    18661 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
    18721 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
    18781 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
    18841 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
    18901 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
    18961 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
    19021 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
    19081 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
    19141 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
    19201 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
    19261 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
    19321 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
    19381 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
    19441 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
    19501 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
    19561 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
    19621 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
    19681 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
    19741 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
    19801 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
    19861 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
    19921 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
    19981 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
    20041 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
    20101 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
    20161 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
    20221 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
    20281 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
    20341 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
    20401 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
    20461 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
    20521 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
    20581 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
    20641 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
    20701 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
    20761 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
    20821 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
    20881 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
    20941 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
    21001 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
    21061 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
    21121 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
    21181 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
    21241 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
    21301 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
    21361 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
    21421 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
    21481 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
    21541 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
    21601 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
    21661 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
    21721 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
    21781 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
    21841 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
    21901 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
    21961 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
    22021 ccaa
//
LOCUS       DACELF010000045        21776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.047, whole genome shotgun sequence.
ACCESSION   DACELF010000045 DACELF010000000
VERSION     DACELF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.047"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IW687_001608"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IW687_001608"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0950872.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IW687_001609"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IW687_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0950873.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IW687_001610"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IW687_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0950874.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IW687_001611"
     CDS             complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IW687_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0950875.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IW687_001612"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IW687_001612"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0950876.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IW687_001613"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IW687_001613"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0950877.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="IW687_001614"
     CDS             complement(8557..9027)
                     /locus_tag="IW687_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0950878.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="IW687_001615"
     CDS             complement(9073..9498)
                     /locus_tag="IW687_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0950879.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="IW687_001616"
     CDS             complement(9565..9834)
                     /locus_tag="IW687_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0950880.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="IW687_001617"
     CDS             complement(9845..11440)
                     /locus_tag="IW687_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0950881.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IW687_001618"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IW687_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0950882.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="IW687_001619"
     CDS             complement(14993..15553)
                     /locus_tag="IW687_001619"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0950883.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="IW687_001620"
     CDS             15906..16880
                     /locus_tag="IW687_001620"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0950884.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="IW687_001621"
     CDS             17050..18450
                     /locus_tag="IW687_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0950885.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="IW687_001622"
     CDS             complement(18528..19250)
                     /locus_tag="IW687_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0950886.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="IW687_001623"
     CDS             complement(19329..19835)
                     /locus_tag="IW687_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0950887.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="IW687_001624"
     CDS             19958..21757
                     /locus_tag="IW687_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0950888.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6038 a         4747 c         3904 g         7087 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttctcctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg ggatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta tacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa ataaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggtcga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctgggaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaata
//
LOCUS       DACELF010000046        20818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.049, whole genome shotgun sequence.
ACCESSION   DACELF010000046 DACELF010000000
VERSION     DACELF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.049"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1241)
                     /locus_tag="IW687_001625"
     CDS             complement(39..1241)
                     /locus_tag="IW687_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0950889.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="IW687_001626"
     CDS             1358..1873
                     /locus_tag="IW687_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0950890.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="IW687_001627"
     CDS             complement(2072..2518)
                     /locus_tag="IW687_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0950891.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW687_001628"
     CDS             complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW687_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0950892.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="IW687_001629"
     CDS             complement(4397..5026)
                     /locus_tag="IW687_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0950893.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5010..5474)
                     /locus_tag="IW687_001630"
     CDS             complement(5010..5474)
                     /locus_tag="IW687_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0950894.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="IW687_001631"
     CDS             complement(5961..7229)
                     /locus_tag="IW687_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0950895.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="IW687_001632"
     CDS             complement(7460..8197)
                     /locus_tag="IW687_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0950896.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="IW687_001633"
     CDS             complement(8264..8833)
                     /locus_tag="IW687_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0950897.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="IW687_001634"
     CDS             8961..9833
                     /locus_tag="IW687_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0950898.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="IW687_001635"
     CDS             10175..11047
                     /locus_tag="IW687_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0950899.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="IW687_001636"
     CDS             11044..13002
                     /locus_tag="IW687_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0950900.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13253..14104)
                     /locus_tag="IW687_001637"
     CDS             complement(13253..14104)
                     /locus_tag="IW687_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0950901.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14227..14694
                     /locus_tag="IW687_001638"
     CDS             14227..14694
                     /locus_tag="IW687_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0950902.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14957..15439)
                     /locus_tag="IW687_001639"
     CDS             complement(14957..15439)
                     /locus_tag="IW687_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0950903.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15539..16402)
                     /locus_tag="IW687_001640"
     CDS             complement(15539..16402)
                     /locus_tag="IW687_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0950904.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17000..17848
                     /locus_tag="IW687_001641"
     CDS             17000..17848
                     /locus_tag="IW687_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950905.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18019..18360)
                     /locus_tag="IW687_001642"
     CDS             complement(18019..18360)
                     /locus_tag="IW687_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0950906.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18446..19153
                     /locus_tag="IW687_001643"
     CDS             18446..19153
                     /locus_tag="IW687_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0950907.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19140..19463
                     /locus_tag="IW687_001644"
     CDS             19140..19463
                     /locus_tag="IW687_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0950908.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19552..20589)
                     /locus_tag="IW687_001645"
     CDS             complement(19552..20589)
                     /locus_tag="IW687_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0950909.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6202 a         4308 c         3695 g         6613 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtaaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccggtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacgtag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttatagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggttgcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gctcgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctcttt
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttactga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcggagg
     7561 tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatcatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cgaaatcatc
     9481 ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaaatattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga tccaacgatt
     9901 aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca
    11881 gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa
    13201 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    13261 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    13321 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    13381 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    13441 cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt
    13501 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    13561 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    13621 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    13681 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    13741 cagtgaattg gtgatctggc gctaatatac tgatcgagtg atcggcatct gcaaaacttg
    13801 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    13861 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    13921 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    13981 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    14041 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    14101 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    14161 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    14221 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    14281 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    14341 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    14401 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    14461 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    14521 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    14581 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    14641 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    14701 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    14761 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    14821 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    14881 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    14941 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    15001 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    15061 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    15121 gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg
    15181 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    15241 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    15301 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    15361 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    15421 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    15481 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    15541 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    15601 attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt
    15661 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    15721 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    15781 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat
    15841 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    15901 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    15961 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    16021 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    16081 atttttgtat gccgtatcta ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    16141 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    16201 atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    16261 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    16321 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    16381 attggttcag atctttaagc atatttatct tcctccttga ttgccttttt atcatattat
    16441 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    16501 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    16561 tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa
    16621 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    16681 attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac
    16741 ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat
    16801 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
    16861 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
    16921 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
    16981 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
    17041 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
    17101 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
    17161 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
    17221 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
    17281 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
    17341 tcttatagaa catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
    17401 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
    17461 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
    17521 tgaatcgctt attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
    17581 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
    17641 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
    17701 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
    17761 ctctataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
    17821 aatagataaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
    17881 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
    17941 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
    18001 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt
    18061 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccgctgc
    18121 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc
    18181 tagtgcagca atcattgttg cgcctaatgc aggtccttcc tccacactta aacaatagac
    18241 cgttgcatcg aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc
    18301 caccgaaaca atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat
    18361 tgacaaatgc tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt
    18421 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
    18481 agacacgtta cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg
    18541 gaaagctgcc ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg
    18601 ttctgcccaa tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt
    18661 tttctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
    18721 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
    18781 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
    18841 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
    18901 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
    18961 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
    19021 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
    19081 caacttagtg attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
    19141 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
    19201 ctagggtcct accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt
    19261 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
    19321 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
    19381 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc
    19441 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
    19501 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
    19561 gaacggacca ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc
    19621 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
    19681 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
    19741 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
    19801 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
    19861 tcggacaagg tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
    19921 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
    19981 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
    20041 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc
    20101 gttgatcact aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt
    20161 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc
    20221 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt
    20281 gcttcctgct cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
    20341 cacttcgtca cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc
    20401 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
    20461 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
    20521 gctttttgga tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt
    20581 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt
    20641 tcaatatata tagtatgcta tttgttccac cttttttatt catcataatt attctcttac
    20701 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
    20761 caacctctta taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattca
//
LOCUS       DACELF010000047        20801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.050, whole genome shotgun sequence.
ACCESSION   DACELF010000047 DACELF010000000
VERSION     DACELF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.050"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW687_001646"
     CDS             <1..300
                     /locus_tag="IW687_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0950910.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IW687_001647"
     CDS             complement(724..894)
                     /locus_tag="IW687_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0950911.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IW687_001648"
     CDS             complement(1088..1267)
                     /locus_tag="IW687_001648"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950912.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1654..2766
                     /locus_tag="IW687_001649"
     CDS             1654..2766
                     /locus_tag="IW687_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0950913.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IW687_001650"
     CDS             complement(2844..3137)
                     /locus_tag="IW687_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0950914.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IW687_001651"
     CDS             3305..3619
                     /locus_tag="IW687_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0950915.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IW687_001652"
     CDS             3640..5619
                     /locus_tag="IW687_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0950916.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IW687_001653"
     CDS             5632..5958
                     /locus_tag="IW687_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0950917.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IW687_001654"
     CDS             5974..6492
                     /locus_tag="IW687_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950918.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IW687_001655"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IW687_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0950919.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IW687_001656"
     CDS             complement(7928..9433)
                     /locus_tag="IW687_001656"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0950920.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IW687_001657"
     CDS             complement(9525..10115)
                     /locus_tag="IW687_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0950921.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IW687_001658"
     CDS             10294..11940
                     /locus_tag="IW687_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0950922.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IW687_001659"
     CDS             11989..12708
                     /locus_tag="IW687_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0950923.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IW687_001660"
     CDS             13187..14134
                     /locus_tag="IW687_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0950924.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IW687_001661"
     CDS             14146..15066
                     /locus_tag="IW687_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0950925.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IW687_001662"
     CDS             15107..16564
                     /locus_tag="IW687_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0950926.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IW687_001663"
     CDS             16909..17523
                     /locus_tag="IW687_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0950927.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IW687_001664"
     CDS             17516..19240
                     /locus_tag="IW687_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0950928.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IW687_001665"
     CDS             19237..20688
                     /locus_tag="IW687_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0950929.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3511 c         4166 g         6415 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atattcatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACELF010000048        20523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.051, whole genome shotgun sequence.
ACCESSION   DACELF010000048 DACELF010000000
VERSION     DACELF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.051"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW687_001666"
     CDS             <1..111
                     /locus_tag="IW687_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0950930.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(569..844)
                     /locus_tag="IW687_001667"
     CDS             complement(569..844)
                     /locus_tag="IW687_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950931.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="IW687_001668"
     CDS             1298..1504
                     /locus_tag="IW687_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950932.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="IW687_001669"
     CDS             1700..1867
                     /locus_tag="IW687_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0950933.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="IW687_001670"
     CDS             1893..2237
                     /locus_tag="IW687_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0950934.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="IW687_001671"
     CDS             2242..2523
                     /locus_tag="IW687_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950935.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="IW687_001672"
     CDS             2626..2940
                     /locus_tag="IW687_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0950936.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="IW687_001673"
     CDS             2918..4612
                     /locus_tag="IW687_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0950937.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="IW687_001674"
     CDS             4632..5810
                     /locus_tag="IW687_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0950938.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="IW687_001675"
     CDS             5773..6459
                     /locus_tag="IW687_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0950939.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="IW687_001676"
     CDS             6459..7619
                     /locus_tag="IW687_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0950940.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="IW687_001677"
     CDS             7629..8504
                     /locus_tag="IW687_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950941.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="IW687_001678"
     CDS             8501..8812
                     /locus_tag="IW687_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950942.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9155
                     /locus_tag="IW687_001679"
     CDS             8802..9155
                     /locus_tag="IW687_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0950943.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9145..9546
                     /locus_tag="IW687_001680"
     CDS             9145..9546
                     /locus_tag="IW687_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950944.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9539..9943
                     /locus_tag="IW687_001681"
     CDS             9539..9943
                     /locus_tag="IW687_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950945.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9955..10563
                     /locus_tag="IW687_001682"
     CDS             9955..10563
                     /locus_tag="IW687_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0950946.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10583..10945
                     /locus_tag="IW687_001683"
     CDS             10583..10945
                     /locus_tag="IW687_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950947.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10948..11130
                     /locus_tag="IW687_001684"
     CDS             10948..11130
                     /locus_tag="IW687_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950948.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11147..14578
                     /locus_tag="IW687_001685"
     CDS             11147..14578
                     /locus_tag="IW687_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0950949.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14629..15366
                     /locus_tag="IW687_001686"
     CDS             14629..15366
                     /locus_tag="IW687_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0950950.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15376..17667
                     /locus_tag="IW687_001687"
     CDS             15376..17667
                     /locus_tag="IW687_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950951.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17691..19817
                     /locus_tag="IW687_001688"
     CDS             17691..19817
                     /locus_tag="IW687_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950952.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19980..20426
                     /locus_tag="IW687_001689"
     CDS             19980..20426
                     /locus_tag="IW687_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950953.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20428..>20523
                     /locus_tag="IW687_001690"
     CDS             20428..>20523
                     /locus_tag="IW687_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0950954.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7229 a         3081 c         4291 g         5922 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     8941 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     9001 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     9061 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     9121 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     9181 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     9241 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     9301 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     9361 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     9421 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     9481 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     9541 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     9601 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     9661 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     9721 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     9781 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     9841 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     9901 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     9961 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
    10021 tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca
    10081 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
    10141 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
    10201 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
    10261 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
    10321 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
    10381 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
    10441 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
    10501 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10561 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10621 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10681 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10741 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10801 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
    10861 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
    10921 taaggaaaaa aagcttcaaa agtaaaaatt ccaaagctct tagttgaaac gtgggtcgat
    10981 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
    11041 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
    11101 tatgcaataa accaatccca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
    11161 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11221 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11281 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
    11341 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg cgaaaaaata
    11401 ttatggagaa aattccaccg aagttaagaa acttgaaaga gaactgatta atcaacaaac
    11461 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11521 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac aaaaaaatgt
    11581 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11641 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11701 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11761 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11821 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11881 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    11941 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
    12001 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12061 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12121 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12181 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12241 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12301 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12361 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12421 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12481 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12541 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12601 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12661 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12721 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12781 gcaagagttg aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
    12841 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12901 taatcttcca caaccagtac aggatttcat tgttgctata ggtggtctta cagcagcatt
    12961 tggcatactg ttaccggcaa tactagccgt gtcatattta tttggtccga tgatgcttat
    13021 aattggagga attatagctg ttatagcagg cgttattgtg gcaattaaga actggggtgc
    13081 aattactgac tggtttagtg gtttatggaa aaaatttact gattggttgg gtgatacttg
    13141 ggaaagtata aaagacggag cctcatcagt ttgggatggg gttaaagaaa cctggtctgg
    13201 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13261 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa cttggcaaac
    13321 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13381 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13441 tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattgg gcgcagcttg
    13501 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13561 atggataagt atcgcttgga atgggattgt aactacattc catactatat ttgatccagt
    13621 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13681 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13741 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13801 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13861 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13921 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    13981 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14041 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14101 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14161 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14221 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14281 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14341 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14401 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14461 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14521 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14581 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14641 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14701 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14761 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14821 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14881 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    14941 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15001 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15061 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15121 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15181 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15241 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15301 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15361 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15421 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15481 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15541 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15601 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15661 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15721 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15781 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15841 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15901 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    15961 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16021 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16081 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16141 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16201 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16261 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16321 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16381 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16441 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16501 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16561 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16621 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16681 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16741 tggacgttag atggcggatt caatgcctcg tttattacgg ctggtgaaat agtaggaatt
    16801 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16861 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16921 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    16981 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17041 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17101 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17161 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17221 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17281 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17341 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17401 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgaaac agtaaatcta
    17461 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17521 gatattgacc atttcatcat tgaaactagt aagccaaacg tattagttag atggaattta
    17581 gtggcacacc gtctagggta tgaagatatt agattaaaag aggatacagc atatgatagc
    17641 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17701 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17761 ctcgtcaagg tgataaaggg attacggtga cggttaaacc gtttctaaat ggcttgcaga
    17821 tggatacgag tggcggaaca tttactttaa aaggaacaac accatctaac cgttacgtag
    17881 ataatgttgc aactagtgta actagtgaag aagtcacgtt ttctcttgat ggcacattta
    17941 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac aatcaaattt
    18001 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18061 ccgatgaata tgtttcgcaa ttagaagagt tgattcgaaa gtacaacgaa acttttgatg
    18121 cttttatggc tgaaatcaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18181 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18241 taggtaactt acaagtgatg tacagtaaca gcatcgactt cggggactat gattattcgg
    18301 agaacccgaa tttaatgcca tatattacag aaccttgggt tggacctttg ttaggtaatg
    18361 gacatacagt taaagatagt gttaaaaggg ttataactca tactaaaact agaacagcta
    18421 attccggcga catattatcg cttggcttag gtattccgtg tacagcggaa gctaataata
    18481 ggtatcttat aaccacttta cgaccatcta cgacatacac attaagcgtt actatgagtg
    18541 tcggttcaga ttggactgga gagaccaata ctataggggt taggcttaga tatttaaatg
    18601 aacaaggtgg tatagaactg cccattaatg cattaatacc agcaaatgta gaacgcgata
    18661 aaatggtcac tcatacattt actggtataa caaaagataa tgtaactagc ataacaaact
    18721 gttatgttga aatattctca cttaatagtg aatataaagg aacggttagt gtcagctatg
    18781 acgttaagtt aaaagcacac taccctaact tactagatgg cccttactgg ttaggtaaag
    18841 ttcctttagg cgagaatatt gctgacccta cagttgtctt tccacacaaa actagtgaat
    18901 atatggttta tggtagacgc aatacagaaa actatatcgc tgaccaaaca tataccatat
    18961 caatgaaagc aactaaacta acagttcaat cgtttgccgt gtatattgct gctggccgcg
    19021 ttaaagtagg agacatgaag cctactgagg ggttagctaa tacatgggag ttaactttta
    19081 ctgtaactaa gcaacatata gatagtgggg taactaatta tctggaaata taccaatacc
    19141 cgtcagctac aaaaggagct gttcaaattg agtggctcaa actagaaaaa ggcaatactc
    19201 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    19261 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    19321 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    19381 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    19441 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    19501 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    19561 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg
    19621 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    19681 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    19741 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    19801 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    19861 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    19921 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    19981 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    20041 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    20101 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    20161 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    20221 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    20281 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    20341 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    20401 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    20461 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    20521 gaa
//
LOCUS       DACELF010000049        19850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.052, whole genome shotgun sequence.
ACCESSION   DACELF010000049 DACELF010000000
VERSION     DACELF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.052"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW687_001691"
     CDS             <1..111
                     /locus_tag="IW687_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0950955.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="IW687_001692"
     CDS             124..510
                     /locus_tag="IW687_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950956.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="IW687_001693"
     CDS             1061..1285
                     /locus_tag="IW687_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950957.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="IW687_001694"
     CDS             1303..1731
                     /locus_tag="IW687_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950958.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="IW687_001695"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="IW687_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ0950959.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="IW687_001696"
     CDS             3139..4674
                     /locus_tag="IW687_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0950960.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="IW687_001697"
     CDS             4754..5632
                     /locus_tag="IW687_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0950961.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="IW687_001698"
     CDS             5634..5951
                     /locus_tag="IW687_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950962.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6000..6701
                     /locus_tag="IW687_001699"
     CDS             6000..6701
                     /locus_tag="IW687_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0950963.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="IW687_001700"
     CDS             6716..7606
                     /locus_tag="IW687_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0950964.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="IW687_001701"
     CDS             7629..7844
                     /locus_tag="IW687_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950965.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="IW687_001702"
     CDS             7856..8197
                     /locus_tag="IW687_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950966.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="IW687_001703"
     CDS             8197..8568
                     /locus_tag="IW687_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950967.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="IW687_001704"
     CDS             8561..8956
                     /locus_tag="IW687_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0950968.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="IW687_001705"
     CDS             8958..9335
                     /locus_tag="IW687_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950969.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="IW687_001706"
     CDS             9336..9944
                     /locus_tag="IW687_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0950970.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10285
                     /locus_tag="IW687_001707"
     CDS             9944..10285
                     /locus_tag="IW687_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950971.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10527..13316
                     /locus_tag="IW687_001708"
     CDS             10527..13316
                     /locus_tag="IW687_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0950972.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13306..14046
                     /locus_tag="IW687_001709"
     CDS             13306..14046
                     /locus_tag="IW687_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0950973.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14043..16805
                     /locus_tag="IW687_001710"
     CDS             14043..16805
                     /locus_tag="IW687_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0950974.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16818..17726
                     /locus_tag="IW687_001711"
     CDS             16818..17726
                     /locus_tag="IW687_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0950975.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            17726..18343
                     /locus_tag="IW687_001712"
     CDS             17726..18343
                     /locus_tag="IW687_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950976.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            18347..18796
                     /locus_tag="IW687_001713"
     CDS             18347..18796
                     /locus_tag="IW687_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950977.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            18796..19320
                     /locus_tag="IW687_001714"
     CDS             18796..19320
                     /locus_tag="IW687_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950978.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            19348..19755
                     /locus_tag="IW687_001715"
     CDS             19348..19755
                     /locus_tag="IW687_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950979.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            19755..>19850
                     /locus_tag="IW687_001716"
     CDS             19755..>19850
                     /locus_tag="IW687_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0950980.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         7089 a         2788 c         4213 g         5760 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
    10321 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
    10381 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
    10441 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
    10501 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    10561 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    10621 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10681 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10741 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10801 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10861 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10921 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10981 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    11041 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    11101 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    11161 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    11221 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    11281 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    11341 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    11401 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    11461 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    11521 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    11581 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    11641 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11701 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11761 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11821 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11881 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11941 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    12001 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    12061 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    12121 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    12181 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    12241 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    12301 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    12361 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    12421 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    12481 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    12541 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    12601 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
    12661 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
    12721 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
    12781 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
    12841 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
    12901 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
    12961 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
    13021 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
    13081 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
    13141 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
    13201 atggagattt acctgattca atggttcgga agattaccac aaaaattaaa acagaattga
    13261 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
    13321 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
    13381 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
    13441 atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg ttactataaa
    13501 gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
    13561 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
    13621 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
    13681 acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
    13741 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
    13801 ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa atcgttggac
    13861 agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga tggtaattta
    13921 gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc tggaaaaaat
    13981 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
    14041 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
    14101 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
    14161 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
    14221 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
    14281 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
    14341 gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata tcaattattg
    14401 aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gccgtttcta
    14461 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
    14521 ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
    14581 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttaaaggtca
    14641 aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct caaagtgagg
    14701 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
    14761 ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa gacattaaga
    14821 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
    14881 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
    14941 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
    15001 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
    15061 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
    15121 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
    15181 caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca aaagaaaaac
    15241 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
    15301 tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt acttcagctt
    15361 ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata
    15421 tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc
    15481 aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga
    15541 attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat ccttttcctg
    15601 gggcttctac tccaacccct attgaagata attctttagt tattcgtaca aatggagacg
    15661 gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta ggaaatggta
    15721 aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa ggaaaagaaa
    15781 taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt gggtatccta
    15841 gtgaattaaa aacagatgca gaaaagagag cttggagaat ttacgatatt ttgtgtaata
    15901 acggatttac gaaacaatct gcatgtggaa tattggggaa cattcaacaa gaaacaggag
    15961 gaacttttga tcctgatact gttcaaatag gtggaccagc atacggatta gttcagtggg
    16021 atggttcttc atatcctctt gttggtccag caacatggga tggaaaagtt tatgttcaaa
    16081 acttatttaa tgctgcaggt attaaagaac caataacgag cttagatgca caagttcgat
    16141 tgcttatttg gacatttaca aatggacaat ggatgggcgt agtacaacct acgacggttg
    16201 atggatttaa ggcctgtact gatccaagac aagcagcata tgcttttgaa cgaaactatg
    16261 aacgtccggc agcgacacat cctgaacgtc aagattatgc agttaactgg tataacaaat
    16321 ttaaagattt aaaaccagga ggagctactg gagaagcagg actaaaacat ttagagtctt
    16381 tgattggaca acgaattggc aatggtcagt gttatggctt gtctgcagaa tattcaggat
    16441 atctgggtgg ttgtggcatg ggtgctggaa caaaatatgg tttaactcat gtgataggaa
    16501 atacttctgc agcatctgat attggtattg cctatgattg gtctgctgtt ggttggaaag
    16561 tgattcaaaa ccctagatat gatcaattag tagttggtgc aattattaat tgggcaagag
    16621 gtggacaagt gggttcatgg tttgcagacg gaacttatgg acatactggc gttatccgag
    16681 gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt atgatttgtg
    16741 gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc ataccaccaa
    16801 aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca gaaccctata
    16861 attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt atggaagcta
    16921 caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat tttgaatcag
    16981 ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct aaaaaaggga
    17041 aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa acagccaatt
    17101 tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc tattttgtca
    17161 tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca attgaagatt
    17221 taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg gactggatga
    17281 atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt ttccaagatt
    17341 ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag gatattttga
    17401 agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt gttgatattc
    17461 aaggagttcg tcatgcgtct atttcagagc gcttgctggc agatatggat tatctatatc
    17521 agaaattacg agcaacactt ttcacgattg aatacggtga gattgaagtg actgatattt
    17581 tgcaggatga tctcttttca gataatcacg aagtcgaaaa agttggaact gtagaatttc
    17641 caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag aatgttttca
    17701 ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg agactgatga
    17761 atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt taggcttaga
    17821 aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg gtgcagtcat
    17881 tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt atgcaagcga
    17941 gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg gagaaggtgg
    18001 aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag ttggggaact
    18061 cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact tcaatttctc
    18121 tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg caaatatatc
    18181 aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg gtgttaatca
    18241 gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaagtt acacaaacga
    18301 aagaattcca aattttactt ttgaaaaaat cggggaggta tagaaaatga cagatatcgt
    18361 aaaaataaaa caaagcggtg tacaggttta tccacaaact cattggaatg ctatagaagg
    18421 caaaccaaca acggtgaagg gagataaagg cgatcctggc caagcagcaa caattaccat
    18481 aggaaccgtt tctagtggtt caaccgcttc tgttacgaat gtaggaacat catctgcagc
    18541 tagatttaat tttgttttac caaagggaga taaaggtgat cctggaataa atgccacaac
    18601 tacagctgta gctacaacta cagcaaatgg gttgatgtcg tccacagata aaactaagtt
    18661 ggatggaatt gcagctggag cacagaaaaa tccaggtaat gctacaacta cgacagcagg
    18721 tttaatgtca gcaaccgata aagtgaaact cgatggatta accaatatta catttgagaa
    18781 agtggggacg gtttaatggc tgatattgtg caattaaaag aagacggagt tgctaagtat
    18841 ttgaaaactc atgccaaagg aattgatggt gtggagggtg ttttagtaaa agccacaggt
    18901 aatgaaacgg tcttaggaac gaaaaatttt aaagacggat tgcaattcaa tggattgcct
    18961 gtgcaagcag gtatgatcga gcgagctatc acactagctg atcgaagtga tacaacaaac
    19021 gtaactgatg tgaatggaaa aataatcaga atcggaaaca tcgtatttct tacttttaat
    19081 tttaaatgtg gcacatggcc cgaaggatca gaaactcgtt ggattttaaa aatcccggac
    19141 ggtttcaaac gtgatcaagg ctatccagcg caaaccgcgc tttcactagt tagaaatgca
    19201 agtcagccag cggatgcacg tgcattcatt gatcaaagta gcatcataca agccaaatca
    19261 ggttcaggta gttcatatat ttcaggtatg tggatcactc aagatccttg gccggcttaa
    19321 cagattacta attggaggaa gagtaaaatg aaaacaattt ataaagtatt gtatccaatg
    19381 ggatatgaag aacatcaagt agaagataac tttccaacat ctttaccatt tgtcgaagtt
    19441 ccacctattt tgtttgaaaa gaaggaagat gaaacggatg aaggtttcgg aagaagacaa
    19501 cagtcgcaat tctttaactt cactgaaaat aagtgggaag aagcagttac acaagattat
    19561 tcaaaaaaat tagaactact agaaaattta tctataggtt tacaggtaga taatgctgca
    19621 ttgaaaaaat caaatgaaga gttaacggaa aaagcagatt cgatggcgca gttgaacgcc
    19681 aaactgatgc ttaatgattt aacaattaat aaaaaaattg aagcaataga aaaacaaatt
    19741 ggaggtgctt cataatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    19801 ttactgacga tcaagttcga caattcgttc cactatgcat tacagacgaa
//
LOCUS       DACELF010000050        19374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.053, whole genome shotgun sequence.
ACCESSION   DACELF010000050 DACELF010000000
VERSION     DACELF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.053"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW687_001717"
     CDS             99..746
                     /locus_tag="IW687_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0950981.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW687_001718"
     CDS             1139..3784
                     /locus_tag="IW687_001718"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0950982.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW687_001719"
     CDS             4094..5407
                     /locus_tag="IW687_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0950983.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW687_001720"
     CDS             5397..6050
                     /locus_tag="IW687_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0950984.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IW687_001721"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IW687_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0950985.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IW687_001722"
     CDS             complement(6671..8023)
                     /locus_tag="IW687_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0950986.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IW687_001723"
     CDS             complement(8089..9378)
                     /locus_tag="IW687_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0950987.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IW687_001724"
     CDS             complement(9465..10169)
                     /locus_tag="IW687_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0950988.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IW687_001725"
     CDS             10369..10635
                     /locus_tag="IW687_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0950989.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IW687_001726"
     CDS             complement(10803..11033)
                     /locus_tag="IW687_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0950990.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IW687_001727"
     CDS             11105..11260
                     /locus_tag="IW687_001727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950991.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IW687_001728"
     CDS             complement(11569..11793)
                     /locus_tag="IW687_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0950992.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IW687_001729"
     CDS             12060..12395
                     /locus_tag="IW687_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0950993.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IW687_001730"
     CDS             12628..13569
                     /locus_tag="IW687_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0950994.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13571..14461
                     /locus_tag="IW687_001731"
     CDS             13571..14461
                     /locus_tag="IW687_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0950995.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IW687_001732"
     CDS             14504..15304
                     /locus_tag="IW687_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0950996.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IW687_001733"
     CDS             15319..16170
                     /locus_tag="IW687_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0950997.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="IW687_001734"
     CDS             16167..16526
                     /locus_tag="IW687_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0950998.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="IW687_001735"
     CDS             16541..16702
                     /locus_tag="IW687_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0950999.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="IW687_001736"
     CDS             16699..17025
                     /locus_tag="IW687_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951000.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="IW687_001737"
     CDS             17022..17837
                     /locus_tag="IW687_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ0951001.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="IW687_001738"
     CDS             17976..18287
                     /locus_tag="IW687_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951002.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="IW687_001739"
     CDS             18284..18514
                     /locus_tag="IW687_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951003.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18521..18724
                     /locus_tag="IW687_001740"
     CDS             18521..18724
                     /locus_tag="IW687_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0951004.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18721..19017
                     /locus_tag="IW687_001741"
     CDS             18721..19017
                     /locus_tag="IW687_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951005.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            19094..>19374
                     /locus_tag="IW687_001742"
     CDS             19094..>19374
                     /locus_tag="IW687_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0951006.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP"
BASE COUNT         6531 a         3213 c         3959 g         5671 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc
    13381 atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc
    13441 tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac aacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
    18541 agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
    18721 atgaaactaa aagacggatt ttacgctagc agtcacggta tcggcggttt aatgctagat
    18781 atgccgacaa agaaccctaa aacacgcaag aaatcaaaat tcaaagtcgg tgacatggtc
    18841 cgctgtgaag cagaagagtt cgtttatccg ttcagaggat acgttaaaaa gatactgtca
    18901 aactcagcaa tcattcgtat tgaaaataca atgaagtgtg atcgttcgac tgcaaaacac
    18961 aagcattatt tagctgtagc gagattagtt gacatggaag taatcaagag caaataaaaa
    19021 aagccggatc gctccgactg attcaataaa tccaacacat ttattatatc acataaagga
    19081 gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa gatgtaatgc
    19141 gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct ctttgataga
    19201 tattaagtcg ccgattataa cggatatgcc gaaggcaccg aagcatggca ataaggcaga
    19261 agacgcgatt attcagatga tggatataga aacggagaga gatgcgattc tagcggcttt
    19321 gatggctctt agtctgatta gtcgtcagat actctactac agcttctgtg tgcc
//
LOCUS       DACELF010000051        19008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.054, whole genome shotgun sequence.
ACCESSION   DACELF010000051 DACELF010000000
VERSION     DACELF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.054"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..271
                     /locus_tag="IW687_001743"
     CDS             <1..271
                     /locus_tag="IW687_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951007.1"
                     /translation="LLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKGIY
                     EMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(368..802)
                     /locus_tag="IW687_001744"
     CDS             complement(368..802)
                     /locus_tag="IW687_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951008.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1034..1744)
                     /locus_tag="IW687_001745"
     CDS             complement(1034..1744)
                     /locus_tag="IW687_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0951009.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1734..2594)
                     /locus_tag="IW687_001746"
     CDS             complement(1734..2594)
                     /locus_tag="IW687_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0951010.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2599..3237)
                     /locus_tag="IW687_001747"
     CDS             complement(2599..3237)
                     /locus_tag="IW687_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0951011.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3252..3551)
                     /locus_tag="IW687_001748"
     CDS             complement(3252..3551)
                     /locus_tag="IW687_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0951012.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3594..5078)
                     /locus_tag="IW687_001749"
     CDS             complement(3594..5078)
                     /locus_tag="IW687_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0951013.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5098..5559)
                     /locus_tag="IW687_001750"
     CDS             complement(5098..5559)
                     /locus_tag="IW687_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0951014.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5577..6644)
                     /gene="ulaG"
                     /locus_tag="IW687_001751"
     CDS             complement(5577..6644)
                     /gene="ulaG"
                     /locus_tag="IW687_001751"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0951015.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6862..7632
                     /locus_tag="IW687_001752"
     CDS             6862..7632
                     /locus_tag="IW687_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0951016.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7905..8732)
                     /locus_tag="IW687_001753"
     CDS             complement(7905..8732)
                     /locus_tag="IW687_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951017.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9300..10535
                     /locus_tag="IW687_001754"
     CDS             9300..10535
                     /locus_tag="IW687_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0951018.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11534..11794)
                     /locus_tag="IW687_001755"
     CDS             complement(11534..11794)
                     /locus_tag="IW687_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0951019.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11821..13122)
                     /locus_tag="IW687_001756"
     CDS             complement(11821..13122)
                     /locus_tag="IW687_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0951020.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13414..13872)
                     /locus_tag="IW687_001757"
     CDS             complement(13414..13872)
                     /locus_tag="IW687_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0951021.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(13885..15210)
                     /locus_tag="IW687_001758"
     CDS             complement(13885..15210)
                     /locus_tag="IW687_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0951022.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15227..16639)
                     /locus_tag="IW687_001759"
     CDS             complement(15227..16639)
                     /locus_tag="IW687_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951023.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(16656..17420)
                     /locus_tag="IW687_001760"
     CDS             complement(16656..17420)
                     /locus_tag="IW687_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0951024.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(17524..18399)
                     /locus_tag="IW687_001761"
     CDS             complement(17524..18399)
                     /locus_tag="IW687_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0951025.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(18654..18738)
                     /locus_tag="IW687_001762"
     tRNA            complement(18654..18738)
                     /locus_tag="IW687_001762"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18702..18704),aa:Leu,seq:caa)
     gene            complement(18756..18827)
                     /locus_tag="IW687_001763"
     tRNA            complement(18756..18827)
                     /locus_tag="IW687_001763"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18793..18795),aa:Gln,seq:ttg)
     gene            complement(18837..18909)
                     /locus_tag="IW687_001764"
     tRNA            complement(18837..18909)
                     /locus_tag="IW687_001764"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18874..18876),aa:His,seq:gtg)
BASE COUNT         5978 a         3980 c         2941 g         6109 t
ORIGIN      
        1 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
       61 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      121 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      181 tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt
      241 tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata
      301 ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta
      361 ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag
      421 aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt
      481 tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca
      541 aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga
      601 tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct
      661 tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg
      721 cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga
      781 tcataaggga atcaatattc atttttttct tcctttctta tacaattctc cctctaataa
      841 tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt
      901 ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat
      961 aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct
     1021 taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt
     1081 tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg
     1141 ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc
     1201 cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt
     1261 ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta
     1321 cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag
     1381 tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat
     1441 gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga
     1501 gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca
     1561 ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc
     1621 ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg
     1681 gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag
     1741 acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct
     1801 cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct
     1861 agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca
     1921 tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca
     1981 gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac
     2041 aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct
     2101 aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg
     2161 ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa
     2221 ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt
     2281 agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct
     2341 ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt
     2401 acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat
     2461 cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct
     2521 tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc
     2581 ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc
     2641 ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc
     2701 gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa
     2761 gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga
     2821 taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc
     2881 catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt
     2941 ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc
     3001 tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac
     3061 tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa
     3121 gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca taacatctcc
     3181 taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt
     3241 agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt
     3301 tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga
     3361 gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt
     3421 tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt
     3481 gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact
     3541 aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact
     3601 ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt
     3661 ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca
     3721 taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc
     3781 ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct
     3841 acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta
     3901 ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg
     3961 atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca
     4021 aacaagaacc caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca
     4081 attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt
     4141 tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa
     4201 gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc
     4261 aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa
     4321 gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt
     4381 tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct
     4441 aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct
     4501 tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct
     4561 aaaatcagtc ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt
     4621 aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa
     4681 cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt
     4741 aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca
     4801 gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt
     4861 ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag
     4921 taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa
     4981 atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt
     5041 gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta
     5101 attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc
     5161 aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc
     5221 tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc
     5281 actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc
     5341 atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc
     5401 gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc
     5461 gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg
     5521 gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat
     5581 aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa
     5641 aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag
     5701 atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc
     5761 tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt
     5821 gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct
     5881 cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg
     5941 gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg
     6001 aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct
     6061 tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg
     6121 atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt
     6181 ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa
     6241 ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg
     6301 attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa
     6361 ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg
     6421 ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct
     6481 tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga
     6541 tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct
     6601 actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca
     6661 tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt
     6721 tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata
     6781 ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat
     6841 gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa
     6901 gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc
     6961 cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag
     7021 atcatacgca attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa
     7081 tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat
     7141 tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa
     7201 tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat
     7261 aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc
     7321 gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc
     7381 ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa
     7441 atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga
     7501 ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa
     7561 ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta
     7621 gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac
     7681 aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt
     7741 atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct
     7801 aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt
     7861 cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt
     7921 tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata
     7981 tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc
     8041 aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat
     8101 aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat
     8161 acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc
     8221 acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca
     8281 gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa
     8341 aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa
     8401 atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta
     8461 taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg
     8521 attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa
     8581 agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt
     8641 gcttgttcac aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat
     8701 tcttttagta atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat
     8761 ttctccttta tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa
     8821 taaaaatttt aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat
     8881 ttctttttgt gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc
     8941 atggcgagaa agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa
     9001 tgacgacagc acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta
     9061 tttttaatga cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt
     9121 gttgcgtgtt tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt
     9181 tgtgaaaaca gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt
     9241 gttttttagt atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca
     9301 tgaagacaat tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg
     9361 aggttgccaa agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt
     9421 ttgctccttt gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga
     9481 aaaaacaaga agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta
     9541 ccaaagaaat ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc
     9601 ctcgactgat tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga
     9661 ttcctttagt atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg
     9721 cgctgatgcc ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga
     9781 caaaaaagcg tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa
     9841 aagaaatgct tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca
     9901 tcacaacgcc taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg
     9961 gtccaatttt tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc
    10021 gaaagcaaca agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc
    10081 gtatgttacg caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac
    10141 ctattgacta tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat
    10201 atctgtctag tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag
    10261 ggaccgtcca aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg
    10321 agcaaagctg taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac
    10381 cagtgactga aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc
    10441 agcaggcacg cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag
    10501 aaatcgaaac cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa
    10561 tagagactca tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta
    10621 aatagtggat tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat
    10681 ccttaaaata aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc
    10741 ttagtcaaat tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa
    10801 agaccataat aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat
    10861 cgctttgtag agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt
    10921 taccacttta tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc
    10981 gatgcatact cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa
    11041 tgttctatgt cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt
    11101 cccagcacaa ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata
    11161 ttaccattca tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata
    11221 tttgacttga tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt
    11281 ccattttttc ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa
    11341 aagggctgtg acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc
    11401 ttgcatctgc ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt
    11461 aagaagcaat tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc
    11521 acttttagaa catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat
    11581 tgatagcaaa aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg
    11641 gtacaatgat ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat
    11701 tgatttgaat atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa
    11761 agataaaaac aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac
    11821 ttagacagtc tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc
    11881 ataagctttt acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa
    11941 gataacaaca cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt
    12001 ccatgttggc aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat
    12061 aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat
    12121 aggagctcca aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg
    12181 accaacctct ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat
    12241 cgttgctcct gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata
    12301 gggtaattgg gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat
    12361 cagccacatt ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca
    12421 caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt
    12481 ggttaaaggc tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg
    12541 aatcagccaa gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc
    12601 aacagcaggt ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg
    12661 atatacttcc actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa
    12721 agaactcata ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg
    12781 aacttgtaac ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc
    12841 atatgattta gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat
    12901 cgtcatccta aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc
    12961 aggaaacggt aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt
    13021 tgaaacaacc ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg
    13081 tgggacaagt ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca
    13141 ataaaatatg aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa
    13201 ttaagaaatt ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg
    13261 aaaataagtt ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa
    13321 atgattaaat ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata
    13381 aatatcaccc tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat
    13441 ctttacttct tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat
    13501 gatcaccgta tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt
    13561 cccttctttt acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc
    13621 aactgtatct aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat
    13681 ggcatgtcgg gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc
    13741 atcatgattc gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt
    13801 tactgctgtt aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt
    13861 aaagaaatgc actttttatc acctctactt taatgctggc caatattttt tgtttacttt
    13921 gatcatttca tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt
    13981 aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc
    14041 tactgcaact tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt
    14101 aattggctcc acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg
    14161 tagattcgga atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt
    14221 caacaaggaa atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa
    14281 tacttcttcc acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg
    14341 tccatccatg accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc
    14401 aggatataag taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat
    14461 gactgacatc atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac
    14521 ttgcatttct tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc
    14581 tttatcataa atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca
    14641 attcttacga tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc
    14701 acaggcatta atcattttta cattcggata tttacgacga acagcttcag aaacgatcga
    14761 ctctggattt gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac
    14821 aatttccaat aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt
    14881 ttcttgtcct actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa
    14941 tcctccaaca cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc
    15001 atccgttgca cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat
    15061 caagtacata tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg
    15121 caaccgtttt tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc
    15181 tccagctata gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata
    15241 atttattgaa ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt
    15301 tttcagttgg tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca
    15361 tggtgggatc cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat
    15421 tggcttttcc tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg
    15481 ctaaccctaa ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac
    15541 gtcctaatgt ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg
    15601 caaaaaagaa aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga
    15661 tgagataatc aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac
    15721 agacaatccc tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa
    15781 acatgaaact aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc
    15841 ttcccatgat tcctttgacc atctctttat tttctttata agctgcttta tagatagcta
    15901 gacatatagc tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag
    15961 cctgaggatc tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag
    16021 atgtatagct tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg
    16081 ggatcagcaa acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga
    16141 gcagttttgc aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa
    16201 cagctgcgat tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat
    16261 aagaaaaaac tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta
    16321 aaccggaaat aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc
    16381 cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat
    16441 ttttgacaaa accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg
    16501 catttccagc tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga
    16561 tcaatcccgc taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca
    16621 catttgattt aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac
    16681 tcaatcaaag gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact
    16741 tggtgggtac gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct
    16801 gcgtgacaca tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa
    16861 tattcttcca cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg
    16921 aaacaaggct caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga
    16981 accggcaaat catatttttt tgctaatgta acaactacct cctgattttc atccaaagta
    17041 tgagtatgat gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga
    17101 atctgagcat tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa
    17161 taagataagg atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt
    17221 ttgccacacg tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt
    17281 ttcacagcat gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct
    17341 cgctgataag catccataat cccataattc acaccatggc tatagccaaa atcatccgaa
    17401 ttaaaaatca cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca
    17461 aataatcata gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt
    17521 attttaatag attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc
    17581 aagttgaaat tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct
    17641 atgtttaaat gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat
    17701 caagcgttta accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt
    17761 atcataaacg tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac
    17821 tatttttaag ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt
    17881 gatgaatcct acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac
    17941 cacttctact gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat
    18001 tgctgtgaca tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa
    18061 tgtaatatag atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac
    18121 ttcttccgca aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt
    18181 gatcttttct ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt
    18241 actcacttta gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc
    18301 atcgatcaat acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc
    18361 tctatatgat aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca
    18421 atttttaaag acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa
    18481 tagtagcacc ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata
    18541 tcttttttac tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa
    18601 aaacaaaaaa aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc
    18661 ggccggggtc gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc
    18721 caattccgcc acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac
    18781 gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg
    18841 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
    18901 cacattcgcc atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca
    18961 tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc ctcaacgg
//
LOCUS       DACELF010000052        18774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.055, whole genome shotgun sequence.
ACCESSION   DACELF010000052 DACELF010000000
VERSION     DACELF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.055"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(566..1132)
                     /locus_tag="IW687_001765"
     CDS             complement(566..1132)
                     /locus_tag="IW687_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0951026.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1132..2088)
                     /locus_tag="IW687_001766"
     CDS             complement(1132..2088)
                     /locus_tag="IW687_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0951027.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2088..2744)
                     /locus_tag="IW687_001767"
     CDS             complement(2088..2744)
                     /locus_tag="IW687_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0951028.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3114..5132
                     /locus_tag="IW687_001768"
     CDS             3114..5132
                     /locus_tag="IW687_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0951029.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5559..7973
                     /locus_tag="IW687_001769"
     CDS             5559..7973
                     /locus_tag="IW687_001769"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0951030.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8321..9874
                     /locus_tag="IW687_001770"
     CDS             8321..9874
                     /locus_tag="IW687_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0951031.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10255..12039)
                     /locus_tag="IW687_001771"
     CDS             complement(10255..12039)
                     /locus_tag="IW687_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951032.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12054..13775)
                     /locus_tag="IW687_001772"
     CDS             complement(12054..13775)
                     /locus_tag="IW687_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951033.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13875..14636
                     /locus_tag="IW687_001773"
     CDS             13875..14636
                     /locus_tag="IW687_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0951034.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15603..15871
                     /locus_tag="IW687_001774"
                     /pseudo
     CDS             15603..15871
                     /locus_tag="IW687_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16543..>18774
                     /locus_tag="IW687_001775"
     CDS             16543..>18774
                     /locus_tag="IW687_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951035.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6173 a         3330 c         3773 g         5498 t
ORIGIN      
        1 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
       61 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      121 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      181 atatttgagg ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat
      241 gaatgacata caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat
      301 tcttttatat atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata
      361 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct
      421 tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga
      481 ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat
      541 atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg
      601 gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt
      661 tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga
      721 cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc
      781 gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga
      841 aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt
      901 gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat
      961 cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg
     1021 ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt
     1081 gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct
     1141 cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc
     1201 aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca
     1261 cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc
     1321 gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc
     1381 ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc
     1441 tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa
     1501 tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc
     1561 taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt
     1621 ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac
     1681 tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa
     1741 attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa
     1801 attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat
     1861 ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc
     1921 agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc
     1981 aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta
     2041 attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc
     2101 tttctgtaag agttatctat ttgttttctt ttttctctat acactggata agtcaggcac
     2161 agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga
     2221 taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg
     2281 cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc
     2341 atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc
     2401 aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt
     2461 acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat
     2521 aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag
     2581 agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg
     2641 aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca
     2701 agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga
     2761 agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca
     2821 atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa
     2881 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
     2941 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
     3001 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
     3061 taagagaagt tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa
     3121 acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac
     3181 gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac
     3241 agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag
     3301 cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat
     3361 atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat
     3421 ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct
     3481 acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac
     3541 aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat
     3601 ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg
     3661 cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata
     3721 atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg
     3781 gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca
     3841 attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg
     3901 attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga
     3961 acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg
     4021 aaaatggctc ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta
     4081 catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt
     4141 ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca
     4201 agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg
     4261 ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa
     4321 gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga
     4381 gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa
     4441 ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca
     4501 cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca
     4561 gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa
     4621 ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca
     4681 tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt
     4741 caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat
     4801 ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca
     4861 aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct
     4921 atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag
     4981 ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca
     5041 gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt
     5101 atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga
     5161 attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa
     5221 gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt
     5281 ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc
     5341 cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg
     5401 tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa
     5461 aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa
     5521 tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa
     5581 ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc
     5641 ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt caagggctac
     5701 atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc
     5761 aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat
     5821 atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc
     5881 gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg
     5941 atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag
     6001 cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg
     6061 aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc
     6121 gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag
     6181 ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag
     6241 caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac
     6301 cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag
     6361 aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa
     6421 aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt
     6481 atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat
     6541 cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat
     6601 tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag
     6661 ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac
     6721 aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag
     6781 tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc
     6841 caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac
     6901 ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata
     6961 tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga
     7021 atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata
     7081 acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca
     7141 ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct
     7201 tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa
     7261 tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg
     7321 atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc
     7381 cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg
     7441 atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat
     7501 tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact
     7561 atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct
     7621 ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga
     7681 ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt
     7741 acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt
     7801 tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg
     7861 agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag
     7921 tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc
     7981 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt
     8041 ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt
     8101 ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct
     8161 gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa
     8221 ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt
     8281 ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat
     8341 tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag
     8401 aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt
     8461 atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt
     8521 aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt
     8581 ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct
     8641 tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct
     8701 ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt
     8761 attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg
     8821 aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc
     8881 ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat
     8941 tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat
     9001 aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa
     9061 tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga
     9121 tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg
     9181 ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg
     9241 gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg
     9301 gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac
     9361 atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt
     9421 ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt
     9481 gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat
     9541 caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg
     9601 tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat
     9661 tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca
     9721 aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc
     9781 agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt
     9841 tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt
     9901 cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt
     9961 aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt
    10021 attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa
    10081 aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca
    10141 aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa
    10201 gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc
    10261 tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga
    10321 atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc
    10381 aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat
    10441 tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat
    10501 aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg
    10561 agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat
    10621 aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat
    10681 tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac
    10741 catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa
    10801 ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt
    10861 tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac
    10921 ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt
    10981 tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc
    11041 taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata
    11101 agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa
    11161 gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa
    11221 ggcgagttga ttaatgaaac gaatggccgg atagatagca aagttcataa attgagcacg
    11281 tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg
    11341 ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa
    11401 aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt
    11461 tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat
    11521 ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat
    11581 acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc
    11641 acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc
    11701 ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa
    11761 tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc
    11821 ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag
    11881 atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa
    11941 actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg
    12001 tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt
    12061 ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta
    12121 ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta
    12181 ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct
    12241 cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc
    12301 tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa
    12361 ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct
    12421 tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc
    12481 atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg
    12541 atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact
    12601 tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat
    12661 ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct
    12721 agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt
    12781 gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct
    12841 gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat
    12901 accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc
    12961 acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa
    13021 gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc
    13081 actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat
    13141 gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg
    13201 gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt
    13261 cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga
    13321 gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt
    13381 tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa
    13441 agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa
    13501 tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag
    13561 gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt
    13621 ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt
    13681 acacctaatg taccggcgat ttgtaaacag atcatcacaa aggtaaggag agcgagtcct
    13741 ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac
    13801 ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa
    13861 agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt
    13921 tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag
    13981 aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt
    14041 tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga
    14101 cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga
    14161 tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata
    14221 caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt
    14281 ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga
    14341 gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac
    14401 atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct
    14461 tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa
    14521 tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg
    14581 atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa
    14641 tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg
    14701 ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat tttagttttt
    14761 tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat atacgatgta
    14821 attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta taattcatgg
    14881 tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg tatcagatga
    14941 gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg ttgcttacat
    15001 tattacaata tgttaataag gcaaaaataa atagatggat tgaataaaca tattttttaa
    15061 tcaaatagtc cctactgtga ctttcctcag ctatcggtga tactaccgat agcatttttt
    15121 tgcttataaa ataattataa gtatttagag atttttcttc taacattctt caaacaagaa
    15181 aaatcacaaa ggttattgtt atttttatat attcttttta agaatgattg tttttttata
    15241 gaaattttaa aaaataaaac acgttattga tggatagtta gtttgttatt tggtaatatt
    15301 atagaagttg actggtagtg atttattttt cgtcgtttta taaaaatgtt attttttgga
    15361 aaaattcaca aatatgtaac gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg
    15421 actaatataa atactattgt catttatgca attttttatg gatagtaatt ttagagggga
    15481 atcaatgaag gaagaataga agggaaaaat agttgcaaat acgccagtta gcaaaaatag
    15541 caatacatct tcaagcttta aaattcgaga gaatcatctt ggtagtggaa tcaactacaa
    15601 caaaaggatt aaaccaatta atagatagaa ttaccaatgg aaaaatagaa aaggtagtta
    15661 ttctatacaa aagttgttta attcgcttcg gttatgaact tagtgaaacc ctttgcaata
    15721 tatggtacag aatatagaaa ttattgacat tacagaaaat gatgaagatt taatacagat
    15781 tgtcactatt tgtagatacc ttttacaagg aaaccgagcc aataaagcta agaagatgat
    15841 taaggagttg atggaagacg atacaagtta agaaagtgaa actacgccca aataaagagc
    15901 aaaaagagta attggacgtg gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt
    15961 attagtaatg acggtttgag aacatccatg acgagaatga aggaacatcc taaatgcgtt
    16021 tagttgaacc aagtgccaaa taatatggcc aaataagcga tcagttatcg atgaatccca
    16081 agtattctaa actatatatt cattttgatg ctaagcattg atatattttt ttgggattga
    16141 tgaaggttaa ccaatacagg gactcactga tgaaattcta gagtctgacc ttggtatcaa
    16201 cgatttagtg attgtttcta atggaaaagt ataaaagtat ataagtacat taacaaaata
    16261 actattaggg ttacgctcca gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata
    16321 attatggtta agccacacgg acaactaaaa gtcatacaaa gctatcgaat caatcaacag
    16381 aagacaatcg gcaatcatta gctttgttta cagcttacat tgcaacgctt gagttgaacc
    16441 gaaaatacga gggaggtaac gcagccattt tgagttgggg aatcttttta gaaaattatc
    16501 gggtaccttg ttgtttagtc aaaattataa gggggagtaa tagtgaggaa caaatttcat
    16561 aaactgttaa gtatgtttct agttatgctt attgtgatca atcaatttgg gaacataacg
    16621 gttctagctg agtctttagc agaacagcca gagcgttcgc tgacgatcga agttgatcaa
    16681 gaggaggctg ctttgggtga gcagttccag ttgcgagtca gaggaacacc agacctgcta
    16741 aatgcattgg tcattgataa gagtgaggat ttgattgaag gggaagtgaa gcaactcaat
    16801 gattacgaac gtctgattac ttattcttcc cagcattcag gtaaatacga attgaccgca
    16861 agaagcggtg atattcaatc aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc
    16921 acaagtgagt cttttaatgt accagaagaa agcacaagtg aatcttttaa tataccagaa
    16981 gaaagcttaa gtgaatcttt taatgaagcc agatcatctg agccatctat aacgcaggaa
    17041 tctcagattg aaagacaaga ggagctcttg aattatgaca tcggcatctc tgctgagcgc
    17101 cccactttgc cgagcaggca tacacaaacg ttaaagggat acattcaagg aggcgctggc
    17161 gatattcttg aagaatatgg ggagatggtc gtacgagttc ctagagcatc gattggctta
    17221 attgagttca ttagagtgga ggtcgctgat gattcacctt taaagccact ggcattttac
    17281 caagaaggca cagacgttgt ttttccattt acctttgatt atgaaggatt aaatgaagcc
    17341 gctcagtcgt atacattcag tatcacgttt gaagttgctt tctttcaatc aggcggtact
    17401 gagccaaata atgactttca agtgagtctt acttatgctg agcgagcagc aaacacggaa
    17461 ttttcaatta cgcctattcc ttcatcaaga cccatttttt cgaaattata ctatggctct
    17521 agagaaatcg acagccaagg aaatcttggg aatctgctga ttgacgggca agacgtgacc
    17581 aacagtaata aatttatttt gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg
    17641 gttaccgatg tactaccgca aggaacaact ttggcaccga atccgactac atggagttcc
    17701 actggaaata gagaacctat ttcaggtggg gttcggatcg cagaagtaac gaatccaggt
    17761 gaagcaaacg ttagaactca ttatgtttcc gaggaatttg tggacaaagt atcttttaat
    17821 aaggaaacga atacacttgt tgtggacttt ggtaatctag cagagggtca atcgtatttt
    17881 attgaatatg ctcttgaagt gaccgatgtc gacttgttca atgaaggaag acaagaggat
    17941 gggttttctc ggaatcgagc gagtatgacc tacaataatc aggtaatgag ccgagactac
    18001 cgcttgagaa tccctgaaag taatcttccg gcaatctctt taagaaaaag tgtggacaaa
    18061 aatgttatca atctcaatga aaatgaacta acgtatactc tgcatgtctc gatgttttct
    18121 ggaacaagaa atacgcttgt tattgaagat gttttggaag aaggcatgga atttgtgtca
    18181 tttctatcag atattccctc tttcattgat gccgaagtca ataatggaat agttcgtttc
    18241 agtgcgaata atacgattgg tggtccgaca agttttaccc tctcttttaa ggtcaatgta
    18301 gaggcgtatg cggtcggcga tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat
    18361 cgattgaata cgagtactgt aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa
    18421 actgatgaga atggccaaag gctggcagga gcaaaatttg aaattttatc aagcgattca
    18481 caagtgatac aagaaggtat aacggcagaa aacggagaat ttttaagtga accgcttcct
    18541 gtaggtagct atcaagtccg agaaacagaa gcaccggaag ggtatctttt agatgcgacg
    18601 gtacatgatg tttttataaa tgaatactcc atagaagaag tgacgttaac cattgaaaac
    18661 atccttgata cgggtggtgt tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt
    18721 caaggagctg tttttgagtt acagaaccga gaaggagaaa cgctacaaac cggt
//
LOCUS       DACELF010000053        18731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.056, whole genome shotgun sequence.
ACCESSION   DACELF010000053 DACELF010000000
VERSION     DACELF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.056"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2939
                     /locus_tag="IW687_001776"
     CDS             <1..2939
                     /locus_tag="IW687_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0951036.1"
                     /translation="DFNNVKFIVSGFSRINGQEYQLLQAMMEKGQLVVDLLLDRPYTA
                     DLPEVLTLFHETGKLYFRLFQSARERKVPILVDRFAPEQTRPTELQLLQTMWEETSSQ
                     KKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRRLVSEENYRYRDIQVLTKNMSLYG
                     NILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLFALDRYSYRLSDIMRFFKTELFLS
                     TEAETELDEWKQERDEFRKKLDITENVALAYNYQGTYWTREKDWQFVAYDFEAEQIED
                     VQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEAANVFYQFLIQSGVQQQLLFWRNQ
                     EVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESAFDWSLFQEIITSGLENLSYGKIP
                     TAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDRKETKGLLSSEEREVLNQALPDGQ
                     FLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQSYDTDGSLKMSPYLRRITEYLSVP
                     VERKEHLTIESDPNCYVGTYRSGINTVNRIYRLALDEKKPVPKHWQKLKELVLNSSWR
                     NFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTSISRMENFYNCEYKYFMQFGLKLK
                     ERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLVNMSDAQRRDFTEKVLQEVFGELR
                     FEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNTGMHPLQTEILFGQIAGAKGITGL
                     ELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDYKSSGRSFDVTEAYYGMAMQLLTY
                     LDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTETDDVEKNTLKKFSYDGVFVDDPAL
                     LEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKDKFFTEEELQLFMAHNRENMRLAG
                     DAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDVMLKENNYHRLEKIDKEEALRRIL
                     KRSEEE"
     gene            2936..6658
                     /gene="addA"
                     /locus_tag="IW687_001777"
     CDS             2936..6658
                     /gene="addA"
                     /locus_tag="IW687_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0951037.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            6727..7068
                     /locus_tag="IW687_001778"
     CDS             6727..7068
                     /locus_tag="IW687_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951038.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            7377..8420
                     /gene="pheS"
                     /locus_tag="IW687_001779"
     CDS             7377..8420
                     /gene="pheS"
                     /locus_tag="IW687_001779"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0951039.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            8425..10845
                     /locus_tag="IW687_001780"
     CDS             8425..10845
                     /locus_tag="IW687_001780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0951040.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11015..12025)
                     /gene="trpS"
                     /locus_tag="IW687_001781"
     CDS             complement(11015..12025)
                     /gene="trpS"
                     /locus_tag="IW687_001781"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0951041.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12279..13016
                     /locus_tag="IW687_001782"
     CDS             12279..13016
                     /locus_tag="IW687_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951042.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13030..13845
                     /locus_tag="IW687_001783"
     CDS             13030..13845
                     /locus_tag="IW687_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0951043.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            13858..14505
                     /locus_tag="IW687_001784"
     CDS             13858..14505
                     /locus_tag="IW687_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0951044.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            14521..15177
                     /locus_tag="IW687_001785"
     CDS             14521..15177
                     /locus_tag="IW687_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0951045.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            15339..16160
                     /gene="racE"
                     /locus_tag="IW687_001786"
     CDS             15339..16160
                     /gene="racE"
                     /locus_tag="IW687_001786"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0951046.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16163..17518
                     /gene="rph"
                     /locus_tag="IW687_001787"
     CDS             16163..17518
                     /gene="rph"
                     /locus_tag="IW687_001787"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0951047.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            17519..18037
                     /locus_tag="IW687_001788"
     CDS             17519..18037
                     /locus_tag="IW687_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0951048.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18144..18644
                     /locus_tag="IW687_001789"
     CDS             18144..18644
                     /locus_tag="IW687_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0951049.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6272 a         3220 c         4039 g         5200 t
ORIGIN      
        1 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
       61 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      121 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      181 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      241 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
      301 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
      361 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
      421 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
      481 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
      541 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
      601 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
      661 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
      721 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
      781 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
      841 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
      901 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
      961 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1021 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1081 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1141 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1201 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1261 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     1321 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     1381 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     1441 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     1501 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     1561 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     1621 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     1681 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     1741 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     1801 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     1861 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     1921 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     1981 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2041 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2101 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2161 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2221 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     2281 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     2341 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     2401 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     2461 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     2521 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     2581 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     2641 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     2701 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     2761 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     2821 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     2881 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     2941 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3001 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3061 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3121 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3181 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3241 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     3301 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     3361 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     3421 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     3481 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     3541 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     3601 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     3661 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     3721 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     3781 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     3841 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     3901 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     3961 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4021 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4081 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4141 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4201 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4261 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     4321 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     4381 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     4441 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     4501 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     4561 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     4621 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     4681 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     4741 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     4801 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     4861 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     4921 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     4981 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5041 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5101 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5161 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5221 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     5281 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     5341 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     5401 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     5461 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     5521 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     5581 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     5641 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     5701 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     5761 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     5821 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     5881 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     5941 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6001 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6061 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6121 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6181 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6241 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     6301 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     6361 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     6421 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     6481 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     6541 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     6601 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     6661 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     6721 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     6781 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     6841 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     6901 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     6961 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7021 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7081 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7141 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7201 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7261 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     7321 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     7381 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     7441 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     7501 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     7561 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     7621 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     7681 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     7741 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     7801 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     7861 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     7921 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     7981 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8041 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8101 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8161 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8221 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     8281 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     8341 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     8401 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     8461 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     8521 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     8581 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     8641 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     8701 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     8761 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     8821 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     8881 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     8941 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9001 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9061 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9121 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9181 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9241 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
     9301 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
     9361 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
     9421 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
     9481 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagaa
     9541 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
     9601 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
     9661 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
     9721 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
     9781 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
     9841 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
     9901 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
     9961 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10021 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10081 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10141 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10201 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10261 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    10321 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    10381 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    10441 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    10501 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    10561 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    10621 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    10681 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    10741 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    10801 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    10861 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    10921 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    10981 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11041 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11101 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11161 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11221 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    11281 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    11341 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    11401 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    11461 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    11521 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    11581 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    11641 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    11701 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    11761 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    11821 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    11881 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    11941 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12001 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12061 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12121 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12181 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12241 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    12301 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    12361 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    12421 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    12481 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    12541 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    12601 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    12661 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    12721 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    12781 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    12841 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    12901 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    12961 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13021 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13081 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13141 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13201 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13261 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    13321 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    13381 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    13441 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    13501 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    13561 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    13621 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    13681 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    13741 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    13801 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    13861 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    13921 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    13981 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14041 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14101 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14161 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14221 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    14281 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    14341 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    14401 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    14461 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    14521 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    14581 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    14641 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    14701 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    14761 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    14821 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    14881 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    14941 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15001 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15061 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15121 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15181 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15241 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    15301 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    15361 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    15421 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    15481 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    15541 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    15601 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    15661 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    15721 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    15781 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    15841 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    15901 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    15961 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16021 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16081 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16141 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16201 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16261 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    16321 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    16381 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    16441 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    16501 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    16561 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    16621 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    16681 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    16741 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    16801 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    16861 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    16921 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    16981 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17041 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17101 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17161 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17221 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    17281 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    17341 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    17401 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    17461 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    17521 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    17581 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    17641 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    17701 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    17761 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    17821 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    17881 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    17941 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18001 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18061 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18121 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18181 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18241 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    18301 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    18361 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    18421 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    18481 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    18541 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    18601 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    18661 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    18721 aggctgggac a
//
LOCUS       DACELF010000054        18676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.057, whole genome shotgun sequence.
ACCESSION   DACELF010000054 DACELF010000000
VERSION     DACELF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.057"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            314..1975
                     /locus_tag="IW687_001790"
     CDS             314..1975
                     /locus_tag="IW687_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0951050.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2045..2392)
                     /locus_tag="IW687_001791"
     CDS             complement(2045..2392)
                     /locus_tag="IW687_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0951051.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2476..3417
                     /locus_tag="IW687_001792"
     CDS             2476..3417
                     /locus_tag="IW687_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951052.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3418..4461
                     /locus_tag="IW687_001793"
     CDS             3418..4461
                     /locus_tag="IW687_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951053.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4467..5537
                     /locus_tag="IW687_001794"
     CDS             4467..5537
                     /locus_tag="IW687_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951054.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5527..6480
                     /locus_tag="IW687_001795"
     CDS             5527..6480
                     /locus_tag="IW687_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0951055.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6492..7502
                     /locus_tag="IW687_001796"
     CDS             6492..7502
                     /locus_tag="IW687_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0951056.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7787..8608)
                     /locus_tag="IW687_001797"
     CDS             complement(7787..8608)
                     /locus_tag="IW687_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0951057.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8705..8983)
                     /locus_tag="IW687_001798"
     CDS             complement(8705..8983)
                     /locus_tag="IW687_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951058.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9251..11260
                     /gene="metG"
                     /locus_tag="IW687_001799"
     CDS             9251..11260
                     /gene="metG"
                     /locus_tag="IW687_001799"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0951059.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11739..12326)
                     /locus_tag="IW687_001800"
     CDS             complement(11739..12326)
                     /locus_tag="IW687_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951060.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12501..13823
                     /locus_tag="IW687_001801"
     CDS             12501..13823
                     /locus_tag="IW687_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0951061.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13978..14754
                     /locus_tag="IW687_001802"
     CDS             13978..14754
                     /locus_tag="IW687_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0951062.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14747..15316
                     /gene="rnmV"
                     /locus_tag="IW687_001803"
     CDS             14747..15316
                     /gene="rnmV"
                     /locus_tag="IW687_001803"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0951063.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15309..16193
                     /gene="rsmA"
                     /locus_tag="IW687_001804"
     CDS             15309..16193
                     /gene="rsmA"
                     /locus_tag="IW687_001804"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0951064.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16644..17273
                     /locus_tag="IW687_001805"
     CDS             16644..17273
                     /locus_tag="IW687_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951065.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17397..18506)
                     /gene="ugpC"
                     /locus_tag="IW687_001806"
     CDS             complement(17397..18506)
                     /gene="ugpC"
                     /locus_tag="IW687_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0951066.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6107 a         3355 c         3793 g         5421 t
ORIGIN      
        1 ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag tttctttttg
       61 tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat tatttaatgg
      121 cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa ttccttgaaa
      181 cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat tttttaataa
      241 ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa attccaataa
      301 aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat gcggccttgt
      361 acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa aaagcggttc
      421 agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta cagctgactt
      481 atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg aaggaattta
      541 ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg cagaagtcag
      601 tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt caaatggtga
      661 tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc cagctacagc
      721 ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta cagctggaaa
      781 gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag aaatcaaatt
      841 gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt tcccacaaaa
      901 acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata aagctgttta
      961 taatggacca ttcacattag aagattttga tggaccagga acagatacag aatggactta
     1021 taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag
     1081 tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag cagatgacgt
     1141 gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata cttctgttaa
     1201 agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa
     1261 tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag taaaacaagt
     1321 actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg taaaaagtcc
     1381 aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg acaaagataa
     1441 agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt tagaatttga
     1501 tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc aaaactcaat
     1561 ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct ctgtacgtct
     1621 tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg ctgactatgc
     1681 agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc gtggtcgctg
     1741 gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg ctggcgatga
     1801 acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg atatgggtgt
     1861 cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt
     1921 tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag aataatcgtt
     1981 aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc gttctcatat
     2041 tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt agtattccgg
     2101 caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag gaaaattctg
     2161 gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga
     2221 aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat gaccataaga
     2281 atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt tttattaaaa
     2341 taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc atttgattgc
     2401 tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga agtcaaaagc
     2461 tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt ctttttcatg
     2521 atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt gctgccgggc
     2581 acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct aaataaacaa
     2641 gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa tttgcttcaa
     2701 ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt gttagctgga
     2761 agaatcggac catccttgca gttagggtta caggcaatta tctttggaac agtcctaggg
     2821 acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac atcttctaca
     2881 ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt attacaatat
     2941 atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt tgtgtacaca
     3001 atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg gtttatccgt
     3061 acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc aaaaggattg
     3121 agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc attgatgact
     3181 ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga aaatatcttt
     3241 gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga ttatcctacg
     3301 atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt gatcgtggat
     3361 ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg aggataaatg
     3421 gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga atttcttcca
     3481 ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt gaattttatc
     3541 caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat ggttttacta
     3601 ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga tccaactgcc
     3661 caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat cgatggattg
     3721 aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc aaacgtccct
     3781 tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt aagccgctta
     3841 tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact agatgtaacg
     3901 attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg
     3961 caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg
     4021 gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac aaactggata
     4081 ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga atatgtttta
     4141 gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat tttgccgaat
     4201 atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat tttcttcgaa
     4261 gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt aggaacgatg
     4321 ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat tccggcagta
     4381 actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg tgatgccttt
     4441 gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa gacttagaaa
     4501 tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat tttcatttga
     4561 ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca gtgacgacca
     4621 gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa atcctcttta
     4681 aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa
     4741 tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc attgggaagc
     4801 aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga caaaaagctg
     4861 cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc aacagttatc
     4921 cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt
     4981 atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact attcaagcac
     5041 agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt atctttatca
     5101 ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa
     5161 gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat ccctatactt
     5221 ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg tatgctattc
     5281 ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat ccaagaaatg
     5341 aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta tcaaaaacac
     5401 acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg ccagaagaaa
     5461 tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt caaggaggaa
     5521 ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt caatgtagga
     5581 aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga aggagaaaca
     5641 ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac gatcattcgc
     5701 ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac gaagatcaaa
     5761 gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca agatccatac
     5821 gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat tgatgttaat
     5881 ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt gagaactgtt
     5941 ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg gcaacgccag
     6001 cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg tgatgaacca
     6061 atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca agatctccaa
     6121 aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt taaacatatt
     6181 agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac aagtgatgaa
     6241 atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat tcctctacca
     6301 gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc agatgacgga
     6361 caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac tgagcaggaa
     6421 gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt tgctaactag
     6481 gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa gaacaaaaac
     6541 agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg ctgaaaaagc
     6601 ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc gctacattaa
     6661 aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat gcacagctta
     6721 atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca aagcttaaaa
     6781 cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg atctatctgg
     6841 aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat cagcatcggc
     6901 cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa gaattttatg
     6961 tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa tggatcggga
     7021 tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta actttaccaa
     7081 aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc
     7141 gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag aaacaaaaga
     7201 gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat cttgaacaat
     7261 tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt ccaaaagaag
     7321 aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa ttgcgttata
     7381 agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca gaagaaagca
     7441 attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt atcaagggat
     7501 aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa aagtcttaat
     7561 gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg acaaaccaat
     7621 aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt ttttattcag
     7681 aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa gagttgtttt
     7741 tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat tcatggtgcc
     7801 ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt tcaccgatct
     7861 catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt ttataatcga
     7921 tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct ttacctaaaa
     7981 cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat gctttcaaga
     8041 tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca gtgactaaat
     8101 caagcgagat agccacttct ccgctagata aatggatctt gttactatga cgttctagtg
     8161 tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct ccaataaagg
     8221 ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta ctgaatgtat
     8281 gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt gtttctctta
     8341 agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat gcccatgaat
     8401 ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct tcttctttgt
     8461 tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct ggacgttcct
     8521 gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa tataattttt
     8581 cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta aaagtacttt
     8641 ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta
     8701 tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa agcagataaa
     8761 acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata acgatatata
     8821 cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa tgcccatgga
     8881 ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt gctaatgaaa
     8941 gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt ccagtccctt
     9001 actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa gccgtttttt
     9061 agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc ttttaaagga
     9121 tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa aaaagctaac
     9181 agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc gataaaggag
     9241 gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct attatccaag
     9301 cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa ttgctcgtta
     9361 taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac atggtcaaaa
     9421 aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc
     9481 ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat tcattcgcac
     9541 gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat tagaacaggg
     9601 agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag aattctttac
     9661 agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc
     9721 gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc gcatgagcaa
     9781 atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc agccagaatc
     9841 tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc ttgcagtttc
     9901 acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg tggtgtatgt
     9961 atggatcgat gcattatcca attatattac tgcactagga tatggctccg aagatgaaag
    10021 tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt
    10081 tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt
    10141 atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt
    10201 cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt attatttgct
    10261 tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg tttcccgtgt
    10321 caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg ctatgatcaa
    10381 caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat ttgatagtga
    10441 actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga
    10501 attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca aatacattga
    10561 tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt
    10621 catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc caatcatgac
    10681 agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa tgaatcttga
    10741 aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag gtacaccaat
    10801 cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg
    10861 tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac
    10921 gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt
    10981 catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac tagatgctgg
    11041 agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg atccaagcga
    11101 attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca
    11161 aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac aagtaatcga
    11221 agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag ggggtatgac
    11281 actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt aaattccttc
    11341 ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa taaatcctag
    11401 aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa taggaatcgt
    11461 aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga ttcatctggt
    11521 actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta ttacatctat
    11581 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
    11641 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
    11701 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
    11761 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
    11821 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
    11881 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
    11941 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
    12001 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
    12061 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
    12121 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
    12181 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
    12241 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
    12301 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
    12361 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
    12421 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
    12481 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
    12541 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
    12601 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
    12661 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
    12721 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
    12781 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
    12841 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
    12901 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
    12961 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
    13021 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
    13081 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
    13141 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
    13201 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
    13261 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
    13321 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
    13381 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
    13441 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
    13501 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
    13561 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt gtctttattt
    13621 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
    13681 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
    13741 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
    13801 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
    13861 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
    13921 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
    13981 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
    14041 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
    14101 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
    14161 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
    14221 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
    14281 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
    14341 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
    14401 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
    14461 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
    14521 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
    14581 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
    14641 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
    14701 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
    14761 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
    14821 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
    14881 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
    14941 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
    15001 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
    15061 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
    15121 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
    15181 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
    15241 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
    15301 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
    15361 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
    15421 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
    15481 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
    15541 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
    15601 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
    15661 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
    15721 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
    15781 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
    15841 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
    15901 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
    15961 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
    16021 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
    16081 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
    16141 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
    16201 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
    16261 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
    16321 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
    16381 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
    16441 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
    16501 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
    16561 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
    16621 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
    16681 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
    16741 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
    16801 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
    16861 tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct tggtacttgc
    16921 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
    16981 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
    17041 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
    17101 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
    17161 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
    17221 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
    17281 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
    17341 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
    17401 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
    17461 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
    17521 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
    17581 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
    17641 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
    17701 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
    17761 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
    17821 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
    17881 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
    17941 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
    18001 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
    18061 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
    18121 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
    18181 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
    18241 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
    18301 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
    18361 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
    18421 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
    18481 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
    18541 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
    18601 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
    18661 actgatacac tggagt
//
LOCUS       DACELF010000055        17516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.058, whole genome shotgun sequence.
ACCESSION   DACELF010000055 DACELF010000000
VERSION     DACELF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.058"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..2427
                     /locus_tag="IW687_001807"
     CDS             100..2427
                     /locus_tag="IW687_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0951067.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IW687_001808"
     CDS             2553..2882
                     /locus_tag="IW687_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0951068.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IW687_001809"
     CDS             2922..3926
                     /locus_tag="IW687_001809"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0951069.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IW687_001810"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IW687_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IW687_001811"
     CDS             5558..6730
                     /locus_tag="IW687_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0951070.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IW687_001812"
     CDS             complement(6892..7347)
                     /locus_tag="IW687_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951071.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IW687_001813"
     CDS             7936..8358
                     /locus_tag="IW687_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0951072.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IW687_001814"
     CDS             complement(8524..9717)
                     /locus_tag="IW687_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0951073.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IW687_001815"
     CDS             9941..10318
                     /locus_tag="IW687_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0951074.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IW687_001816"
     CDS             10306..10644
                     /locus_tag="IW687_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0951075.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IW687_001817"
     CDS             10778..11221
                     /locus_tag="IW687_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0951076.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IW687_001818"
     CDS             11483..12298
                     /locus_tag="IW687_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0951077.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IW687_001819"
     CDS             12308..12970
                     /locus_tag="IW687_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0951078.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IW687_001820"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IW687_001820"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0951079.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IW687_001821"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IW687_001821"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0951080.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IW687_001822"
     CDS             15692..16189
                     /locus_tag="IW687_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0951081.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IW687_001823"
     CDS             16374..16634
                     /locus_tag="IW687_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951082.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IW687_001824"
     CDS             complement(16996..17235)
                     /locus_tag="IW687_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951083.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3473 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DACELF010000056        17265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.059, whole genome shotgun sequence.
ACCESSION   DACELF010000056 DACELF010000000
VERSION     DACELF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.059"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1717)
                     /locus_tag="IW687_001825"
     CDS             complement(137..1717)
                     /locus_tag="IW687_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0951084.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2118..3494)
                     /locus_tag="IW687_001826"
     CDS             complement(2118..3494)
                     /locus_tag="IW687_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0951085.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3622..4740)
                     /locus_tag="IW687_001827"
     CDS             complement(3622..4740)
                     /locus_tag="IW687_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0951086.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4882..5316
                     /locus_tag="IW687_001828"
     CDS             4882..5316
                     /locus_tag="IW687_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951087.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5394..5927)
                     /locus_tag="IW687_001829"
     CDS             complement(5394..5927)
                     /locus_tag="IW687_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0951088.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="IW687_001830"
     CDS             complement(6021..7199)
                     /gene="mutY"
                     /locus_tag="IW687_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0951089.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7192..7989)
                     /gene="recX"
                     /locus_tag="IW687_001831"
     CDS             complement(7192..7989)
                     /gene="recX"
                     /locus_tag="IW687_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0951090.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8096..9478
                     /gene="rlmD"
                     /locus_tag="IW687_001832"
     CDS             8096..9478
                     /gene="rlmD"
                     /locus_tag="IW687_001832"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0951091.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9652..10230)
                     /locus_tag="IW687_001833"
     CDS             complement(9652..10230)
                     /locus_tag="IW687_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0951092.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10552..11022
                     /locus_tag="IW687_001834"
     CDS             10552..11022
                     /locus_tag="IW687_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0951093.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11130..12356)
                     /locus_tag="IW687_001835"
     CDS             complement(11130..12356)
                     /locus_tag="IW687_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0951094.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13110..13310)
                     /locus_tag="IW687_001836"
     CDS             complement(13110..13310)
                     /locus_tag="IW687_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0951095.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13444..14052)
                     /locus_tag="IW687_001837"
     CDS             complement(13444..14052)
                     /locus_tag="IW687_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0951096.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14137..14607)
                     /locus_tag="IW687_001838"
     CDS             complement(14137..14607)
                     /locus_tag="IW687_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0951097.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14655..15569)
                     /locus_tag="IW687_001839"
     CDS             complement(14655..15569)
                     /locus_tag="IW687_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0951098.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15715..16518
                     /locus_tag="IW687_001840"
     CDS             15715..16518
                     /locus_tag="IW687_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0951099.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16696..>17265
                     /locus_tag="IW687_001841"
     CDS             16696..>17265
                     /locus_tag="IW687_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0951100.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPE"
BASE COUNT         5226 a         3460 c         2836 g         5743 t
ORIGIN      
        1 agacatttct atgattttaa tatagtaaaa agagccgtca cggaaaatcc gtcacggctc
       61 taaacaaaat atgagaggct gtttttgttt ggctattctt attacttgat gcagggcagc
      121 ttgtgtcaag acttctttac atcaaacttt ttaattttac attgggatat ttatccgcaa
      181 accagcgttc tgcaaattgg ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag
      241 ccaagatatt gcgacttgaa ctcatcttct catccaaatc ttctggatcg atccagcgag
      301 cgattttgtg tcccattgga gacatgatta cttcggcgtt atactcattt aacatacgat
      361 attggaatac ttcgaattgt agctgaccaa cagctccaat gatatattct tccgttagat
      421 atgttttata aagctgaatc gctccttctt gaactaattg atagattcct ttgtggaagg
      481 atttttgttt catgacattt tttgcagtca ctttcataaa cagctctgga gtaaatgaag
      541 ggagttcttc ataagcaact tttagttttc cttcgaacaa tgtatcccca atctgataat
      601 tgcctgtatc atatacaccg atgatatcgc ctgctactgc tttttcgacg ttttctctcg
      661 aatctgccat aaattgtgtg acattgctta atttcagttt tttatttgta cgttccaacc
      721 agacatccat tcctcgttcg aatgttccag aacagattcg gacaaaagcg attcggtcac
      781 gatgggctgg attcatattt gcttggatct tgaatacgaa acctgaaaat tcttcctcat
      841 aaggacttac catctgatca tcttgtgttt tgtgtccata aggagctggt gccaattcaa
      901 cgaaagtctc taaaaaagtc tgtacaccga agttagtcaa tgcagaacca aaaaatactg
      961 gtgtctgatc gccacgagca attttttctt gattaaatgc atctccagcc tctttgatta
     1021 gttctacttc ttctaagact tgttcatata aggaattttc agttagagga tgattttcgg
     1081 gaagcagtcc atcttcatcc aattctacta atcgttcatc ctgataattt tccggacgat
     1141 aaaactctac acgattatgg tagatatcat aaagaccttc tagcccttta cccataccga
     1201 ttggccaatt catcggataa gattcgatat ctaatagctc ttctaattct tctaacaatt
     1261 ccaatggttc acgaccatca cgatctagtt tgttgataaa agtaaatatc gggatgccac
     1321 gtttttttac aacttggaat aatttctttg tctgtgcttc gatccctttt gcactatcga
     1381 ttaccatgac cgcactatcc acggccatca aagtacgata agtatcttca gaaaaatctt
     1441 catgtcctgg tgtatccaag atatttactc gtttcccttg ataatcaaac tgcataacag
     1501 aactagtaac agaaatccca cgttgtttct cgatttccat ccagtctgat ttggcaaaat
     1561 tacctgtttt tttgcctttt acggtcccgg cttgacggat agccccacca aacaatagta
     1621 attgttctgt aatcgttgtt ttcccagcat ccggatggga aatgatcgca aaagttctgc
     1681 gattgtccac ttgttgcttt aattttggat tattcataac ctaactccat ttcttccatt
     1741 caattattta tattttctct aggcacagat tgtccgatct cttaaagaaa gacaatttca
     1801 tatagggctg tgacaaaagc cgtgtagcag tctctttttc cgagtaaacg gcgttctaga
     1861 gacagcctat cagcaataag cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg
     1921 ctgctcttat tacttaatgc aggatagctt tttcacaacc tttgtcaact actacaactg
     1981 aatcagtata gcatggattc agcgaatgaa aaagccacca aaccataaga gtcaaatatt
     2041 tttttgtcag ctgactaaaa gaaaagacgt gttgcttgtt tattcaagca acacgtcttt
     2101 cataaccaag tttccaatta tcttcttggc tcgtcatctt cgaattcttt tctgaagtat
     2161 cttcgttctt cttcaggttc cgcttctgtt tcttccggat catgaaaaat gacacggatt
     2221 ttaaggattc gagaaccttc catttcttca gaaaccagcg taatattatc gacatcaaac
     2281 gataactttt cgccttcatc cggaatcatg cctaatgctg taatcaaata accagccatc
     2341 gtatctacat cactcatatg cagatcactg tcaaaagctt cattgaactc atcaatcagc
     2401 atacgtcctt ggatgatata ttcgtgttca tcgatttttt cataaagatt ctcaacttca
     2461 tctgattcat catcgatttc tccgacgatt tcttctaaaa ggtcttctag tgttgctaac
     2521 ccaacgacgc cgccatattc atctaataaa atagccatct gattctgcgt ctttttcatt
     2581 tcatataaca gatcatcaat aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt
     2641 ttcttgatat ctaaattatc aaagccgaat ttatgggcag cttttagtaa attctttgta
     2701 tgaaggattc cgactacttt atctttgtct tcgttataaa cagggattct tgaatagttt
     2761 tcggataaaa cttcattcac attttcttcc acagtgtcgt tgatatcaat cataaatgca
     2821 tccgttcgtg ggaccatgac ttctcgggca actttcgtat caagagaaaa aaccccttgg
     2881 agcatttcta attcttcatt attcaatacg ccttcgtttt caagcatata acgcatttca
     2941 tctctggtca tttttgaatc ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa
     3001 cttgtggaag cagaaagcag ccagacgaag ggcttagcta ctacaccaat caagcgtatc
     3061 agccctgaag tgaactttgc gacttcttct gatttattta aagcaatacg tttagggtac
     3121 aattctccaa aaacaatcga tacataggtc aaaatagcca gcacaatgat atttgcaata
     3181 ttttttgccg ctgtgccgcc acctaatacc ggagctaacc gttccgataa agtattagct
     3241 agtgaagcac cagacaaaat gttgaccaaa gtgattccta cttggattgt cgataggaaa
     3301 ttatttggat tctgcaatat ttttaaaagc ttttgagatt tcacatctcc ttcatcagct
     3361 ttttgctcga ttcgattctt attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa
     3421 gcattgatca atgtcaaaac gatcaataat aaaatctgcg ctaacagcga ctgactctca
     3481 gggtcagcat tcataatcgc attctctcct tataaaatta atttttagtt tacgatagaa
     3541 aaaaacgtgg cactatagcc acgttaaaat ggtatcacaa agcaattttc tttgcaataa
     3601 aaacaagtgt actaaaacta cctacgaggt cgtagcaatt ttcttcttgt tttcattttg
     3661 cttgtcttct ttgacgattt tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa
     3721 aggaacacct aagaagatac caagcaatcc ggcaatattt ccagctacta gaagaacgat
     3781 aatgatcgtt aatggatgga ttttcagtga tttcccaatt acattaggat aaatgatatt
     3841 cccatccaac tgttggacga ttagcacaac cacacaacaa agtaatgctt ttaccggatc
     3901 attgaagact gttactagaa atgcaggtgc tagtcctaga taaggtccta aatatggaat
     3961 taggtttgtc aatcccgcaa tgaccccaaa taagaaagca taatccacac caattaccat
     4021 atatcctaaa aatgtgaagg taccaacgaa taaacattcg attgcttgtc cgctaatgta
     4081 attagccaat gttttattca gttgccccaa taactccaca atttggtttc ttcttttttc
     4141 tgggaaaaac tgttggatgt ttggcactag tttttctccg tctttcaaca tatagaacaa
     4201 gatgaatggt gctgtaatga ttactacggt tgccgaagcg atcgttccaa cgatcgaacc
     4261 taaactattg gatactccac taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa
     4321 ttgctcaaaa tatttattga tatctacttc cttgaacaaa ggcaattcgg ctacctcttt
     4381 taaccagctt tctacctgtt tgatgaaatc aggcatatta gatgccaatg aggaaatttg
     4441 ctgtactaga cttgggatga cactaagtaa aatccaaata attgctgcaa ctaaaaggag
     4501 cagcacaatt gcgacagccc atattctttt gatttttgct ttcatcaaca gcttgacaat
     4561 aggattcaat aaatagtaaa gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa
     4621 aaatgtcccg atcggttcga aaataaagtt tatttctgat gaaatcatga tcaatgcagc
     4681 tatgatcaat aattcaagcg accaaaacat tagtttagac tgtctaagtt tttcaaacaa
     4741 tagatttcct cctttctttc ttactagtat atattatatt cgttaaaaat tcttttattc
     4801 tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata
     4861 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
     4921 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg
     4981 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
     5041 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
     5101 cggatggcga ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa
     5161 gcagaaaact ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca
     5221 gctgaaactt tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct
     5281 ggcatgcctc atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa
     5341 aacagcaagc ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta
     5401 cgcgacaatt gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca
     5461 tctccattat tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata
     5521 tcgttcgggt aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt
     5581 ttctcgccgt caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca
     5641 tcgtccagca aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc
     5701 attgctatta cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt
     5761 acccatcggc gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag
     5821 tgttcgcttg tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc
     5881 ttgtaacttt ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct
     5941 taagcagcat tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata
     6001 tcaataaaaa tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga
     6061 tctaccagtt tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac
     6121 cacttcgaag ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa
     6181 aaaaggagaa catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt
     6241 gtctgccaaa caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact
     6301 aggtcatcaa acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac
     6361 tcctcttgtg tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta
     6421 tctctttctt caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa
     6481 acgtcttttg gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg
     6541 ttagctaaac aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag
     6601 atagctgaac ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg
     6661 attttccgca tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca
     6721 atgcaaaaca atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt
     6781 ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta
     6841 atttcttcag gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca
     6901 gcttggatgt tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt
     6961 tcttctggag catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa
     7021 taatcgatca ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga
     7081 taaggatcac gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg
     7141 aactgatctt gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact
     7201 ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata
     7261 taagctttgc tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca
     7321 tagtttatct ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg
     7381 aagatcagct atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg
     7441 ttggattttt ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc
     7501 ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc
     7561 aatgatttca ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt
     7621 gtcacttaag cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag
     7681 gtccaattct tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc
     7741 ttttaagtat gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc
     7801 taggtgaaaa ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc
     7861 taattcccgt ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc
     7921 ttctccttca gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac
     7981 ggttaacata aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt
     8041 aatcaggtat tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc
     8101 agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa
     8161 tggtgaagga atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa
     8221 agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat
     8281 aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg
     8341 cggtggctgt caattacaac acttagctta tcaagcgcaa ttggatttta aaaaggatct
     8401 gcttttgcaa gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc
     8461 gacgatcggg atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa
     8521 aaataagcag actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt
     8581 ccctataaca gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt
     8641 tcaactattg aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt
     8701 ccgcacaatc atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac
     8761 tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc
     8821 cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga
     8881 tgaaacgatc cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt
     8941 cgatctttct cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg
     9001 cgaaggaatc aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg
     9061 agttgggacg atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac
     9121 cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac
     9181 tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca
     9241 gcctgatggt atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc
     9301 attattgaaa taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc
     9361 cagagatctg gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat
     9421 gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat
     9481 gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata
     9541 gctcttcatg ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt
     9601 tatttaaaaa aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat
     9661 attcacgtct atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa
     9721 aatcacatta tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg
     9781 tggaacagtt gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg
     9841 ttgattagga ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg
     9901 tgctgttaaa ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct
     9961 gttagatgtc tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc
    10021 atcaatgaat gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac
    10081 attttgattt aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt
    10141 aatataacca tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc
    10201 aataaacaat aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat
    10261 tgtaaacgct ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa
    10321 aaaaggtgaa aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag
    10381 aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt
    10441 tcaacttttt aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt
    10501 cttgctgtga gccttcatgg taaactacta ttatagaaag gagttagtaa aatgaagatg
    10561 ataaaagata ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca
    10621 gggttgctta gcggaaatcc cggagaatat tactattctt tgcaacttcc accatttgca
    10681 ccacctagct ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct
    10741 ctttatttgt tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt
    10801 gtgattcagt tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt
    10861 ttcattgcag caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag
    10921 ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta
    10981 ttcgcaactc tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc
    11041 ctgtaattta acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg
    11101 gcactcttta ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta
    11161 ttctggtcat aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta
    11221 tcttgacttg gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga
    11281 tcttggtact gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta
    11341 ttcgatccat caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa
    11401 gcattctcat ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc
    11461 agttcatttt ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg
    11521 atcatttgtc cttcgccttg caactggtct tgtttgattg tttcaaacgc aggggtgtag
    11581 ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact
    11641 gcttttaaaa tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt
    11701 ttcgtcacta cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt
    11761 cgcattcgag aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca
    11821 tggacaccat ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg
    11881 atcaaatctt ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc
    11941 agttgctcaa ttgaatccat ggccatttcg acaccaccaa atgcataggc atgatttaat
    12001 ttatctaccg ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc
    12061 gttgattttt tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt
    12121 tccgttcttc ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg
    12181 tcatcaatgc ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt
    12241 ttgtttactg tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca
    12301 atcagcaaaa caaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata
    12361 cacctctgtt tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca
    12421 ctttttaatc ataagaaaac tttaaaaaac atttttgtgt aacgattact attttttaaa
    12481 atttctcaaa attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca
    12541 cgcagttcgc aatcgtttta tcttttttta cgtatttttt ccagaaaaag cgaacaacaa
    12601 ggtattttcc aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac
    12661 agtctttgcc taccgttcag attatcattc gatttcacga aaatttcttc ctatgactgg
    12721 tccttttgtt aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa
    12781 atgactcgaa ctagtgattt cattaccatc atcttttaat ggatagatat gctcctcttc
    12841 aaagtaaaac gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta
    12901 gtggtttcac aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat
    12961 tactaacgaa gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat
    13021 gcatttactt agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct
    13081 aagaagtcgt gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt
    13141 ccgcgtgcac cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag
    13201 ccgtctcctt gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata
    13261 aacccaaatc ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatag
    13321 cctccaaaaa agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc
    13381 tagcaagctt tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa
    13441 tggttatttt gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa
    13501 tgctgcaata taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac
    13561 gtccaaacct agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga
    13621 ggtaatagct aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc
    13681 agcggctgct tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat
    13741 cgcttctttt atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa
    13801 aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg
    13861 aatagcgtcc atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt
    13921 ctcaattgct gcatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg
    13981 catcgtttct tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa
    14041 tgtgtaagtc ataaaaaaat tcctcctcaa tgttttcttt ataatgtcta cattacaaag
    14101 agtaacaaag agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc
    14161 tacttcttct tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt
    14221 cgctaggata acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac
    14281 attgatcaac aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa
    14341 gaacttaccg cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca
    14401 aagtttgttc gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa
    14461 ggccatactt aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt
    14521 agccaaacca ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata
    14581 tccttttaac tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta
    14641 tcatactcta tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc
    14701 tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa
    14761 gaaattatac gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata
    14821 tgtccgtcga aaataattat cttttcgcta tactgccaaa tatggacatg atgaacattc
    14881 tttaccccat cgattgcttc aatttcttgt tccatttttt catagtcaat agcaggtgca
    14941 ctttgtatca aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat
    15001 atcgaaatga tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa
    15061 attgctccga taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta
    15121 gattttatat tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc
    15181 gcgagcaatc caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa
    15241 gtgaaccgct tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag
    15301 ttgatgaaag cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct
    15361 tcttttcggg ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg
    15421 ttatggaaag catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct
    15481 gataaagtga tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt
    15541 ggctgtgtag gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga
    15601 ataacatttt tttgatattt caccaatcat tcagatgttt ccctaaagca cttgtatttc
    15661 acttgatact tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca
    15721 acaaaacaat taattatcag ttcctttata cgattcaaag acctaaagga gacgagaaat
    15781 gtcccatttt ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca
    15841 atctcttttc aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct
    15901 actcgcattc ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga
    15961 ttcatttacc aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa
    16021 caggacatct ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa
    16081 cttatcatag ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta
    16141 gaacttccct acaccaatga atctttaaaa aacttaacag gtaaggagct ccgttctttt
    16201 ttaagtaaag catctatacc actttggata aaagtcctga catttatatt ttctatttat
    16261 cctagttttc tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat
    16321 gcacgctttc gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca
    16381 atttcattgc ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacaca
    16441 agcgccttca ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca
    16501 aaaatatcga tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt
    16561 tagaggttgt gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc
    16621 aaaaaacaag caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa
    16681 ggcgggaaat taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa
    16741 tttatcgaag aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca
    16801 gaggatctac aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta
    16861 ttagcctctt ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc
    16921 cttgagactt tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa
    16981 tcgattacgg agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca
    17041 caaaaagcac agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa
    17101 ggaaaaaaaa tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc
    17161 aaagcattag acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg
    17221 gaaaagactt tttctttaaa tcaactaaag gaagttcttc ctgaa
//
LOCUS       DACELF010000057        17073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.060, whole genome shotgun sequence.
ACCESSION   DACELF010000057 DACELF010000000
VERSION     DACELF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.060"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..530)
                     /locus_tag="IW687_001842"
     CDS             complement(9..530)
                     /locus_tag="IW687_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0951101.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IW687_001843"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IW687_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0951102.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IW687_001844"
     CDS             4610..5497
                     /locus_tag="IW687_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0951103.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IW687_001845"
     CDS             5494..6663
                     /locus_tag="IW687_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951104.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IW687_001846"
     CDS             6687..8042
                     /locus_tag="IW687_001846"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0951105.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IW687_001847"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IW687_001847"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0951106.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="IW687_001848"
     CDS             10328..10618
                     /locus_tag="IW687_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951107.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="IW687_001849"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="IW687_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0951108.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="IW687_001850"
     CDS             11659..12318
                     /locus_tag="IW687_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0951109.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="IW687_001851"
     CDS             12475..13407
                     /locus_tag="IW687_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951110.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /locus_tag="IW687_001852"
     CDS             13561..14850
                     /locus_tag="IW687_001852"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0951111.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="IW687_001853"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="IW687_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0951112.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /locus_tag="IW687_001854"
     CDS             16430..17017
                     /locus_tag="IW687_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0951113.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5586 a         3060 c         3739 g         4688 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agt
//
LOCUS       DACELF010000058        16735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.061, whole genome shotgun sequence.
ACCESSION   DACELF010000058 DACELF010000000
VERSION     DACELF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.061"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW687_001855"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW687_001855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0951114.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2423..3118)
                     /locus_tag="IW687_001856"
     CDS             complement(2423..3118)
                     /locus_tag="IW687_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0951115.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IW687_001857"
     CDS             complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IW687_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0951116.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4317..4508)
                     /locus_tag="IW687_001858"
     CDS             complement(4317..4508)
                     /locus_tag="IW687_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0951117.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4529..5419)
                     /locus_tag="IW687_001859"
     CDS             complement(4529..5419)
                     /locus_tag="IW687_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0951118.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5406..6173)
                     /locus_tag="IW687_001860"
     CDS             complement(5406..6173)
                     /locus_tag="IW687_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0951119.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IW687_001861"
     CDS             complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IW687_001861"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0951120.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IW687_001862"
     CDS             complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IW687_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0951121.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IW687_001863"
     CDS             complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IW687_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0951122.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10789..11127
                     /locus_tag="IW687_001864"
     CDS             10789..11127
                     /locus_tag="IW687_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951123.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(11295..12053)
                     /locus_tag="IW687_001865"
     CDS             complement(11295..12053)
                     /locus_tag="IW687_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0951124.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(12071..12889)
                     /locus_tag="IW687_001866"
     CDS             complement(12071..12889)
                     /locus_tag="IW687_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0951125.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IW687_001867"
     CDS             complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IW687_001867"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0951126.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IW687_001868"
     CDS             complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IW687_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0951127.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            14017..15351
                     /gene="dnaA"
                     /locus_tag="IW687_001869"
     CDS             14017..15351
                     /gene="dnaA"
                     /locus_tag="IW687_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0951128.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15549..16679
                     /gene="dnaN"
                     /locus_tag="IW687_001870"
     CDS             15549..16679
                     /gene="dnaN"
                     /locus_tag="IW687_001870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0951129.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4624 a         3538 c         3005 g         5568 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa
     2221 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     2281 cttttgaaca ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta
     2341 ctattattta actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa
     2401 aatagctgat ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt
     2461 attttttctt caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta
     2521 ctggatctaa tactggattg ataactggtg tcaatagtgt tgtcgcagaa aacagtacga
     2581 cccaaagaag tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc
     2641 ttcgatctgc tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc
     2701 agccaccgat tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac
     2761 gtgaaaacaa tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat
     2821 tatctaacca catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga
     2881 ttgcttccgt tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt
     2941 gttctgcaac taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca
     3001 aattcgctgc ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta
     3061 attttgtttc taaactattg ttttctttta cgattgctcg taattgttct ggttccatta
     3121 tctaatagtc ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca
     3181 tattctacag ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc
     3241 gtcttgtacg acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg
     3301 catgtttccg taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg
     3361 ttcgaaatct gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt
     3421 ccaagcacgt acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata
     3481 agcagcacgg atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc
     3541 cgctttgtct tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat
     3601 cacttctgca ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc
     3661 tgcatctgct acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa
     3721 taaaaatagt gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga
     3781 ttttccttct tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc
     3841 tttatctttg gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa
     3901 gtctgctaac actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc
     3961 ttctacgtga gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac
     4021 ttggcggata tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac
     4081 gattccagca atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag
     4141 ttctgttaag cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat
     4201 cgtcgcaaag ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt
     4261 tgattttcct acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac
     4321 tctttctctg catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc
     4381 atcatgacga tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg
     4441 acttcccagc gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag
     4501 tcgtacataa aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat
     4561 caagaattcg agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat
     4621 ttttttcttg gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc
     4681 ggatataata tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat
     4741 tttgatttaa ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg
     4801 cacgattggc taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt
     4861 gtcccataga taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca
     4921 agcgcaaata attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt
     4981 gtgttagttt caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca
     5041 gatcttctcg ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt
     5101 ctctaacgat agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct
     5161 cccctgcaat aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga
     5221 cacctgattg ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac
     5281 ggggctgata cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt
     5341 ttacatccac cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta
     5401 atcctttact tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct
     5461 cctcgagaac gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa
     5521 cgaatatttc ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct
     5581 tctacgactt catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg
     5641 atctctaatt ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt
     5701 aatccttcca atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta
     5761 aatgaattga ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac
     5821 tgactgctta cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc
     5881 aattcgatct ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt
     5941 tcagtgatca aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct
     6001 cttgtcacat caggttttct gatcccgaca ccactggtag catttccttg tgcatccata
     6061 tccactagca atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc
     6121 gttgttttgc cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct
     6181 cctcctattt gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg
     6241 ttggtttctt tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa
     6301 ccgcctcttc tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca
     6361 attcctcttc actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg
     6421 gcaaacacaa ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa
     6481 atgatgcacg aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag
     6541 catcgattcc taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat
     6601 caacgatcgt tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg
     6661 cccccgaacc aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg
     6721 taagtgaatc gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt
     6781 tcatcttttc gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat
     6841 gatcggtaag ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa
     6901 atattccttt cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa
     6961 aatctctaga catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc
     7021 cgatagactt tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt
     7081 tgagcaattt ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt
     7141 cactgacttt cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag
     7201 ggtttacgcc actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt
     7261 tttgttttgc ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct
     7321 ttttcgcttc catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt
     7381 catatttgat ttggacttct acttgttcga tttcttttcg tgtcaattct tcattttctg
     7441 gaataaaccg tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta
     7501 gtacaccatc ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg
     7561 ttggtttgat tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct
     7621 tcaaataatt cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta
     7681 atcgcaaatc tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca
     7741 aacgataagg ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac
     7801 cgtcacttcg tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac
     7861 cggcaatcag cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag
     7921 ctgtataaag attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta
     7981 ctacgtcata ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa
     8041 tagaatgaag catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat
     8101 agacttcttc cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg
     8161 caaaacgcac gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga
     8221 tccctgtaaa cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac
     8281 ctgtataggt cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga
     8341 agctaaaatg attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg
     8401 tacttgattt tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt
     8461 cttttagatg attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt
     8521 tcaattcacc gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt
     8581 tggcccggta gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga
     8641 tcagttcttc gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt
     8701 tcatttctgt tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc
     8761 cttttcctgt gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt
     8821 ctccgcctaa cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag
     8881 gattacatgg catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc
     8941 ccatacgtgc agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca
     9001 cgtcatacgc atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa
     9061 caaaattgac tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct
     9121 cctagatagt cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa
     9181 tcaatacctg caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg
     9241 cgggtattag aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca
     9301 gaaatagctg attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact
     9361 ggttcatttt cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt
     9421 ttatttagaa gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct
     9481 gctgtcagtg gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct
     9541 tttcggcttc tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct
     9601 gtatcgatta gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga
     9661 gtcgttcctg caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa
     9721 cttgatttgc cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc
     9781 ttcccttgct tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt
     9841 tcaagcaata acttcgtagt caattcttcg acatcatcat attctggata atcaatattt
     9901 acttctactt gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac
     9961 agattgccgt ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc
    10021 aaatccatca cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt
    10081 gtgaattcgc ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga
    10141 ttgactacaa cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc
    10201 tttggtgctc tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca
    10261 acgatatgtc cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat
    10321 tgataaactt tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt
    10381 gcaccttctc caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca
    10441 gccatttttt cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta
    10501 tgcacaataa acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat
    10561 agattatcac acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct
    10621 gttctgcttc cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt
    10681 tattcgaaga gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac
    10741 taaatggtag cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc
    10801 agatctgctg cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca
    10861 gggaatatca accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa
    10921 gctgatttgt ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa
    10981 ttaaagacga aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat
    11041 cagcaaaaaa aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc
    11101 caatttatcc aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga
    11161 agacagtttc gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga
    11221 taagaatgat ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc
    11281 aaaaagtaat ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt
    11341 tcctcacctt ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt
    11401 ttacgttcaa atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt
    11461 tcagccgtgc gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct
    11521 acattgacta ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga
    11581 tattgcaatg cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt
    11641 tctaaatgga aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca
    11701 tcaagctgtt ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca
    11761 cctagctcac ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc
    11821 tcagctgttg gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact
    11881 ttttcgctga tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa
    11941 cctttttttc cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca
    12001 gccagaccta gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt
    12061 acctccccta ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc
    12121 tttcgcggat tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga
    12181 tcagcagcgt ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc
    12241 tggctaaatt gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca
    12301 ttgatgcatt cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga
    12361 ttgctgctaa aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga
    12421 aatgcccttg tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg
    12481 gcatttgtac taataaagga agacaaccag catatggatt gacgccattt tctgcatata
    12541 aacgctgctg ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt
    12601 gctgcagtgc ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga
    12661 aatgcatcaa cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc
    12721 ctgcattacc aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat
    12781 cccaaattcc tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga
    12841 ccaaactaac gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc
    12901 ctctctgtcg attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc
    12961 ataagtcaac cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat
    13021 ttcttctttt atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac
    13081 cgcgttgccg accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt
    13141 ttccaaacgg taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc
    13201 ttgaaactct cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc
    13261 tatctctcac ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct
    13321 aaaaaagagt ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct
    13381 tcctttgcga cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg
    13441 gaatccgtga actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc
    13501 cacctccaaa atgttcttat ctattttata aacacagaca tactttgata gtataacgac
    13561 aaagtagtga ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat
    13621 tatttttttc tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata
    13681 ataaaaggct ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg
    13741 atttcctgtg tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc
    13801 aagaggaaat ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact
    13861 ttttttgaca actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat
    13921 taaaaaattt ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac
    13981 tagttattca ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg
    14041 aatgaattaa aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc
    14101 gaaacagcga accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag
    14161 attcataaag aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg
    14221 ttatcaggaa atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa
    14281 gttatagaag aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg
    14341 aaccctaaat atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca
    14401 gcagcgctcg ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga
    14461 ggcgtgggac ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt
    14521 cagcctaatg ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat
    14581 tcgatccaaa acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg
    14641 ctagtggatg acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat
    14701 actttcaatg ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca
    14761 aacgagattc cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt
    14821 gatattacac cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct
    14881 gaacgtctag agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac
    14941 attcgtgaat tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa
    15001 gatatttcga ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat
    15061 cctcaattgt caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg
    15121 aaagatctca aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg
    15181 tatttggcac gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc
    15241 aaggatcata caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt
    15301 gtcagtatgc aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat
    15361 tctatcaact gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact
    15421 ttttccacag ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac
    15481 atgccctatt acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga
    15541 gatacatcat gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc
    15601 aacgagctat ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga
    15661 cacaagaagg tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt
    15721 ctgttgaaaa cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag
    15781 cacgtttctt tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt
    15841 tagaaaataa acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag
    15901 atgcagataa ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg
    15961 tacacgtatt gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc
    16021 gtccaatctt gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta
    16081 ctgattctca ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg
    16141 atattgttat tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag
    16201 aaatcgtcga aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt
    16261 tctattcccg tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt
    16321 ttaatacaga ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt
    16381 tactttctca tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg
    16441 ttttatacgg aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa
    16501 gcagtggcga tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg
    16561 catttggaga tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg
    16621 aaccaacgga agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac
    16681 aatgattttc aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaat
//
LOCUS       DACELF010000059        16150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.062, whole genome shotgun sequence.
ACCESSION   DACELF010000059 DACELF010000000
VERSION     DACELF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.062"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(282..1592)
                     /locus_tag="IW687_001871"
     CDS             complement(282..1592)
                     /locus_tag="IW687_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0951130.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(1774..3003)
                     /gene="rpsA"
                     /locus_tag="IW687_001872"
     CDS             complement(1774..3003)
                     /gene="rpsA"
                     /locus_tag="IW687_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0951131.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3138..3818)
                     /locus_tag="IW687_001873"
     CDS             complement(3138..3818)
                     /locus_tag="IW687_001873"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0951132.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(3867..4502)
                     /locus_tag="IW687_001874"
     CDS             complement(3867..4502)
                     /locus_tag="IW687_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0951133.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4568..5971)
                     /locus_tag="IW687_001875"
     CDS             complement(4568..5971)
                     /locus_tag="IW687_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0951134.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(5958..6989)
                     /locus_tag="IW687_001876"
     CDS             complement(5958..6989)
                     /locus_tag="IW687_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951135.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7020..7241
                     /locus_tag="IW687_001877"
     CDS             7020..7241
                     /locus_tag="IW687_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0951136.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7406..8143)
                     /locus_tag="IW687_001878"
     CDS             complement(7406..8143)
                     /locus_tag="IW687_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0951137.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8296..9636)
                     /locus_tag="IW687_001879"
     CDS             complement(8296..9636)
                     /locus_tag="IW687_001879"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0951138.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(9873..11396)
                     /locus_tag="IW687_001880"
     CDS             complement(9873..11396)
                     /locus_tag="IW687_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0951139.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(11774..12385)
                     /locus_tag="IW687_001881"
     CDS             complement(11774..12385)
                     /locus_tag="IW687_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0951140.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12508..12633)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12715..13431)
                     /locus_tag="IW687_001882"
     CDS             complement(12715..13431)
                     /locus_tag="IW687_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0951141.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13437..14006)
                     /gene="scpB"
                     /locus_tag="IW687_001883"
     CDS             complement(13437..14006)
                     /gene="scpB"
                     /locus_tag="IW687_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0951142.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(13990..14787)
                     /locus_tag="IW687_001884"
     CDS             complement(13990..14787)
                     /locus_tag="IW687_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0951143.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(14757..15128)
                     /locus_tag="IW687_001885"
     CDS             complement(14757..15128)
                     /locus_tag="IW687_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0951144.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15147..16034)
                     /gene="xerD"
                     /locus_tag="IW687_001886"
     CDS             complement(15147..16034)
                     /gene="xerD"
                     /locus_tag="IW687_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0951145.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4540 a         3504 c         2731 g         5375 t
ORIGIN      
        1 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
       61 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
      121 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
      181 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
      241 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
      301 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
      361 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
      421 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
      481 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
      541 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
      601 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
      661 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
      721 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
      781 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
      841 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
      901 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
      961 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     1021 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     1081 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     1141 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     1201 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     1261 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     1321 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     1381 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     1441 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     1501 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     1561 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     1621 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     1681 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     1741 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     1801 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     1861 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     1921 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     1981 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     2041 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     2101 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     2161 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     2221 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     2281 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     2341 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     2401 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     2461 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     2521 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     2581 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     2641 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     2701 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     2761 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     2821 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     2881 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     2941 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     3001 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     3061 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     3121 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
     3181 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
     3241 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
     3301 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
     3361 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
     3421 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
     3481 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
     3541 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
     3601 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
     3661 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
     3721 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
     3781 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
     3841 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
     3901 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
     3961 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
     4021 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
     4081 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
     4141 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
     4201 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
     4261 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
     4321 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
     4381 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
     4441 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
     4501 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
     4561 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
     4621 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
     4681 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
     4741 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
     4801 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
     4861 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
     4921 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
     4981 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
     5041 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
     5101 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
     5161 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
     5221 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
     5281 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
     5341 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
     5401 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
     5461 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
     5521 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
     5581 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
     5641 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
     5701 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
     5761 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
     5821 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
     5881 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
     5941 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
     6001 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
     6061 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
     6121 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
     6181 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
     6241 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
     6301 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
     6361 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
     6421 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
     6481 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
     6541 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
     6601 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
     6661 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
     6721 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
     6781 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
     6841 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
     6901 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
     6961 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
     7021 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
     7081 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
     7141 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
     7201 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
     7261 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
     7321 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
     7381 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
     7441 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
     7501 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
     7561 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
     7621 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
     7681 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
     7741 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
     7801 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
     7861 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
     7921 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
     7981 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
     8041 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
     8101 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
     8161 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
     8221 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
     8281 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
     8341 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
     8401 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
     8461 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
     8521 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
     8581 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
     8641 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
     8701 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
     8761 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
     8821 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
     8881 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
     8941 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
     9001 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
     9061 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
     9121 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
     9181 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
     9241 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
     9301 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
     9361 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
     9421 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
     9481 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
     9541 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
     9601 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
     9661 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
     9721 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
     9781 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
     9841 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
     9901 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
     9961 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    10021 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    10081 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    10141 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    10201 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    10261 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    10321 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    10381 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    10441 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    10501 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    10561 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    10621 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    10681 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    10741 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    10801 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    10861 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    10921 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    10981 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    11041 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    11101 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    11161 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    11221 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    11281 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    11341 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    11401 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    11461 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    11521 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    11581 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    11641 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    11701 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    11761 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    11821 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    11881 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    11941 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    12001 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    12061 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    12121 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    12181 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    12241 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    12301 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    12361 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    12421 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    12481 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    12541 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    12601 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    12661 ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt
    12721 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    12781 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    12841 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    12901 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    12961 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    13021 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    13081 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    13141 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    13201 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    13261 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    13321 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    13381 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    13441 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    13501 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    13561 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    13621 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    13681 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    13741 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    13801 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    13861 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    13921 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    13981 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    14041 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    14101 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    14161 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    14221 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    14281 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    14341 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    14401 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    14461 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    14521 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    14581 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    14641 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    14701 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    14761 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    14821 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    14881 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    14941 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    15001 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    15061 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    15121 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    15181 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    15241 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    15301 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    15361 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    15421 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    15481 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    15541 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    15601 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    15661 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    15721 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    15781 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    15841 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    15901 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    15961 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    16021 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    16081 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    16141 ggtttctttt
//
LOCUS       DACELF010000060        15878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.063, whole genome shotgun sequence.
ACCESSION   DACELF010000060 DACELF010000000
VERSION     DACELF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.063"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..2604
                     /locus_tag="IW687_001887"
     CDS             133..2604
                     /locus_tag="IW687_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0951146.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="IW687_001888"
     CDS             complement(2707..4443)
                     /locus_tag="IW687_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0951147.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="IW687_001889"
     CDS             complement(4440..5144)
                     /locus_tag="IW687_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0951148.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="IW687_001890"
     CDS             5275..5778
                     /locus_tag="IW687_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0951149.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="IW687_001891"
     CDS             5738..6076
                     /locus_tag="IW687_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0951150.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="IW687_001892"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="IW687_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0951151.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="IW687_001893"
     CDS             7627..8205
                     /locus_tag="IW687_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0951152.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="IW687_001894"
     CDS             8209..8730
                     /locus_tag="IW687_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0951153.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="IW687_001895"
     CDS             complement(8809..9363)
                     /locus_tag="IW687_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0951154.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="IW687_001896"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="IW687_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0951155.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="IW687_001897"
     CDS             9903..10154
                     /locus_tag="IW687_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0951156.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="IW687_001898"
     CDS             10279..11070
                     /locus_tag="IW687_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951157.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..11761
                     /gene="rimM"
                     /locus_tag="IW687_001899"
     CDS             11240..11761
                     /gene="rimM"
                     /locus_tag="IW687_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0951158.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11751..12512
                     /gene="trmD"
                     /locus_tag="IW687_001900"
     CDS             11751..12512
                     /gene="trmD"
                     /locus_tag="IW687_001900"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0951159.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12648..12995
                     /gene="rplS"
                     /locus_tag="IW687_001901"
     CDS             12648..12995
                     /gene="rplS"
                     /locus_tag="IW687_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0951160.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13253..13546
                     /locus_tag="IW687_001902"
     CDS             13253..13546
                     /locus_tag="IW687_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0951161.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            14404..15591
                     /locus_tag="IW687_001903"
     CDS             14404..15591
                     /locus_tag="IW687_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0951162.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         5292 a         2843 c         3236 g         4507 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    11521 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    11581 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    11641 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    11701 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    11761 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    11821 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    11881 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    11941 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    12001 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    12061 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    12121 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    12181 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    12241 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    12301 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    12361 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    12421 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    12481 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    12541 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    12601 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    12661 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    12721 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    12781 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    12841 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    12901 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    12961 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    13021 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    13081 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    13141 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    13201 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    13261 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    13321 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    13381 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    13441 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    13501 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    13561 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    13621 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    13681 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    13741 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    13801 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    13861 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    13921 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    13981 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    14041 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    14101 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    14161 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    14221 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    14281 aaaaagagaa aaaaagatta cgtccatata attgtgtaaa agtgaagcat caaaaaagcc
    14341 attgatctca gttaggagta caatgttttt tgcagaaaac atactcgaat aggactgata
    14401 tcaatgactc aagtacattt tacttttgaa agcgaagaaa ttcaagcaat tataaacgaa
    14461 agcggtgcga atgataccgc aaaaacactt atgactatca tgtttaatca gctgatggaa
    14521 gaacaacgaa atcaatatat tcaggcaaat gcctatgaac gttcagaaga acggcaatca
    14581 caaagaaatg gttactacga tcgttcgttt acaactcgaa ttggtacgtt agaacttcat
    14641 gttccacgaa ccagagacgg taagtttagt cctactatct ttgaaagata tcagcgaagc
    14701 gaaaaagctt tgatcgctgc catgattgaa atggtcattt ctggtgtctc cactcgcaaa
    14761 gtaactaaaa cagttgaact gctaacggat ggtgcgactg tttctaaatc attcgtttca
    14821 aatctgatga aacagttgga tccatttgtt tttgagtgga gaaaccggag cctagaaggc
    14881 tcggaatatc catttttcat gtgtgatgcc ctgtatatga aagtaaggga aaatcatcgt
    14941 attgtctcaa aaggtgtata tattggtatc ggcattgatt ctgatggacg acgtacgatt
    15001 cttggttttg atgttcaaga cggcgaatcg gaagataatt gggataccgt ttttcaatcg
    15061 ttcgttcaac gaggtttgtt cggtgtaaaa ctggtcattt ctgatgcaca caaagggtta
    15121 gttaaggctg ttcgcaagaa ctttttaggt gcgagctggc aaagatgcca agcccacttt
    15181 ttgagaaata tttttgataa gctgccaaag aaagtttctt ctgatgtaaa agacgagttg
    15241 aagagtatct ttaaagcctc tgaacttgaa ttgacacgtg aaagaaaaga gcacttcttg
    15301 gaaaaatatg gttgtgattc aaaattaagt gctgcttgcg acattttaga aaacggcttt
    15361 gaagatgcta ttcaaatttt gtcttttcct gaaaatatcc gccgaaggat tcgtacaacc
    15421 aatgtgttgg aacggttgaa cgaagagatt cgccggagag aacgagtgat tcggattttt
    15481 ccaaatatca attccatcac tcggataatt ggaacacttt tgatggaaaa agacactgag
    15541 tggctggctt ctccacgaaa atatttagaa tttaattcga ataacattta gcaccatgaa
    15601 cctgtgcgag tctcttcttt ctgaggagag acatcaagag tcgctgcgct actgctcctg
    15661 acctctctcc tcagaaagaa aggcatggca gttaggttca caacgcaaaa cattgtgctt
    15721 ttgatgaggt tgaactttta cacaagattg tggacttgac tgtctcatta aaaagcataa
    15781 ccattactcc gatctgtttt taaaattagt ttctacctct aatatatatc gagtttttat
    15841 tttgccttca gagaatactg tttctttttt tgcagtta
//
LOCUS       DACELF010000061        15850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.064, whole genome shotgun sequence.
ACCESSION   DACELF010000061 DACELF010000000
VERSION     DACELF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.064"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..482)
                     /locus_tag="IW687_001904"
     CDS             complement(12..482)
                     /locus_tag="IW687_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0951163.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IW687_001905"
     CDS             complement(641..1099)
                     /locus_tag="IW687_001905"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0951164.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IW687_001906"
     CDS             complement(1270..2415)
                     /locus_tag="IW687_001906"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0951165.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IW687_001907"
     CDS             complement(2428..3345)
                     /locus_tag="IW687_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0951166.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IW687_001908"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IW687_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0951167.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IW687_001909"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IW687_001909"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0951168.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IW687_001910"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IW687_001910"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0951169.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IW687_001911"
     CDS             6871..7752
                     /locus_tag="IW687_001911"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0951170.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IW687_001912"
     CDS             7749..8465
                     /locus_tag="IW687_001912"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0951171.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IW687_001913"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IW687_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0951172.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IW687_001914"
     CDS             9182..10183
                     /locus_tag="IW687_001914"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0951173.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IW687_001915"
     CDS             10180..11541
                     /locus_tag="IW687_001915"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0951174.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IW687_001916"
     CDS             11501..12652
                     /locus_tag="IW687_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0951175.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IW687_001917"
     CDS             12627..13796
                     /locus_tag="IW687_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0951176.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IW687_001918"
     CDS             13971..15350
                     /locus_tag="IW687_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0951177.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15850
                     /locus_tag="IW687_001919"
                     /pseudo
     CDS             15631..15850
                     /locus_tag="IW687_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5181 a         2798 c         3374 g         4497 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatttg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc
//
LOCUS       DACELF010000062        15826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.065, whole genome shotgun sequence.
ACCESSION   DACELF010000062 DACELF010000000
VERSION     DACELF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.065"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(266..466)
                     /locus_tag="IW687_001920"
     CDS             complement(266..466)
                     /locus_tag="IW687_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0951178.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IW687_001921"
     CDS             complement(771..2003)
                     /locus_tag="IW687_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0951179.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2260..2829
                     /locus_tag="IW687_001922"
     CDS             2260..2829
                     /locus_tag="IW687_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0951180.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3007..3447)
                     /locus_tag="IW687_001923"
     CDS             complement(3007..3447)
                     /locus_tag="IW687_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0951181.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3605..4369
                     /gene="map"
                     /locus_tag="IW687_001924"
     CDS             3605..4369
                     /gene="map"
                     /locus_tag="IW687_001924"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0951182.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4401..5324
                     /locus_tag="IW687_001925"
     CDS             4401..5324
                     /locus_tag="IW687_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0951183.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5400..6539
                     /locus_tag="IW687_001926"
     CDS             5400..6539
                     /locus_tag="IW687_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0951184.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6532..7332
                     /locus_tag="IW687_001927"
     CDS             6532..7332
                     /locus_tag="IW687_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0951185.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7332..8159
                     /locus_tag="IW687_001928"
     CDS             7332..8159
                     /locus_tag="IW687_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0951186.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8137..8871
                     /locus_tag="IW687_001929"
     CDS             8137..8871
                     /locus_tag="IW687_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0951187.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8971..9837
                     /gene="rfbA"
                     /locus_tag="IW687_001930"
     CDS             8971..9837
                     /gene="rfbA"
                     /locus_tag="IW687_001930"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0951188.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9851..10423
                     /gene="rfbC"
                     /locus_tag="IW687_001931"
     CDS             9851..10423
                     /gene="rfbC"
                     /locus_tag="IW687_001931"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0951189.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10445..11473
                     /gene="rfbB"
                     /locus_tag="IW687_001932"
     CDS             10445..11473
                     /gene="rfbB"
                     /locus_tag="IW687_001932"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0951190.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11571..12422
                     /gene="rfbD"
                     /locus_tag="IW687_001933"
     CDS             11571..12422
                     /gene="rfbD"
                     /locus_tag="IW687_001933"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0951191.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12456..14489
                     /locus_tag="IW687_001934"
     CDS             12456..14489
                     /locus_tag="IW687_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951192.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14533..15813
                     /locus_tag="IW687_001935"
     CDS             14533..15813
                     /locus_tag="IW687_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0951193.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4860 a         2732 c         3283 g         4951 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aatgactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg cagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccag ccgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg
     2101 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
     2161 gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg
     2221 gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt
     2281 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     2341 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     2401 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     2461 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     2521 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     2581 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     2641 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     2701 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     2761 attgaaaaga tgatacaaga cagtatagat gctatcttga aattcaaaga accaatagaa
     2821 atcgattgaa tatactcaaa agttccacaa atcagtagat aacaagagat gtgtcaaaat
     2881 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     2941 gaatgcaatg aataaaagag ccgtacagat tgaataaatc tggatggctc ttttattgca
     3001 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     3061 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     3121 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     3181 tccgcagaca ccgtaaattt ttcccgatag atccaattct tgcaagtctt cataaaagtc
     3241 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     3301 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     3361 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     3421 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     3481 tatgaattat accaaaacat cattgaaaag aaaataaaac ctagagacac ctggggaatg
     3541 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     3601 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     3661 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     3721 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     3781 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     3841 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     3901 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     3961 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     4021 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     4081 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     4141 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     4201 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     4261 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     4321 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     4381 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     4441 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     4501 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     4561 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     4621 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     4681 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     4741 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     4801 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     4861 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     4921 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     4981 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     5041 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     5101 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     5161 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     5221 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     5281 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     5341 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     5401 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     5461 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     5521 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     5581 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     5641 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     5701 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     5761 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     5821 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     5881 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     5941 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     6001 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     6061 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     6121 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     6181 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     6241 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     6301 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     6361 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     6421 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     6481 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     6541 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     6601 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     6661 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     6721 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     6781 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     6841 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     6901 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     6961 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     7021 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     7081 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     7141 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     7201 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     7261 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     7321 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     7381 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     7441 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     7501 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     7561 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     7621 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     7681 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     7741 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     7801 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     7861 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     7921 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     7981 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     8041 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     8101 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     8161 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     8221 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     8281 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     8341 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     8401 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     8461 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     8521 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     8581 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     8641 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     8701 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     8761 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     8821 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     8881 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     8941 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     9001 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     9061 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     9121 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     9181 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     9241 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     9301 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     9361 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     9421 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     9481 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     9541 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     9601 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     9661 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     9721 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     9781 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     9841 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     9901 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     9961 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    10021 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    10081 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    10141 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    10201 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    10261 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    10321 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    10381 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    10441 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    10501 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    10561 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    10621 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    10681 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    10741 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    10801 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    10861 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    10921 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    10981 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    11041 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    11101 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    11161 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    11221 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
    11281 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
    11341 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    11401 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    11461 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
    11521 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
    11581 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
    11641 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
    11701 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
    11761 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
    11821 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
    11881 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    11941 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    12001 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    12061 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    12121 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    12181 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
    12241 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    12301 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
    12361 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    12421 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    12481 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
    12541 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    12601 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    12661 ctgatgtgat ttttggacag aatcgggagg tgcggtcaga cgaatggctg gtgcagacac
    12721 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
    12781 acggacaaaa catgatcatt gcgtataatt ctcctgcaaa ggatattacg gttatcggaa
    12841 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    12901 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    12961 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    13021 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
    13081 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    13141 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    13201 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    13261 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    13321 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    13381 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    13441 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    13501 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    13561 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    13621 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    13681 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    13741 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    13801 catggtctta tttctatgcg attacgcatt cgaaaataga taattatttt tcggattttg
    13861 aaaccatcgc agttgctgtg ttgggaacag tgattttagc atttgcatta tttggctgga
    13921 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
    13981 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    14041 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    14101 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    14161 aggaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    14221 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    14281 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    14341 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    14401 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    14461 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    14521 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    14581 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc agggttaggt
    14641 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    14701 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    14761 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    14821 ggagatttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    14881 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    14941 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    15001 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    15061 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
    15121 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
    15181 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    15241 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    15301 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    15361 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccaa
    15421 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    15481 ttgataggat ttgggattgc tactttgctg cttatccttt ttattggtcg cttaacgttg
    15541 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    15601 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    15661 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    15721 gaagaggcat atgacgatat gacttatgat gatacatctt atggggaaca accagatagc
    15781 gctcattctt ccaggatcca acgctatcat tgataggcac aaaata
//
LOCUS       DACELF010000063        15545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.066, whole genome shotgun sequence.
ACCESSION   DACELF010000063 DACELF010000000
VERSION     DACELF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.066"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..469
                     /locus_tag="IW687_001936"
     CDS             59..469
                     /locus_tag="IW687_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0951194.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            462..1163
                     /locus_tag="IW687_001937"
     CDS             462..1163
                     /locus_tag="IW687_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0951195.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1163..2776
                     /locus_tag="IW687_001938"
     CDS             1163..2776
                     /locus_tag="IW687_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0951196.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            2766..2987
                     /locus_tag="IW687_001939"
     CDS             2766..2987
                     /locus_tag="IW687_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0951197.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2984..3745
                     /locus_tag="IW687_001940"
     CDS             2984..3745
                     /locus_tag="IW687_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0951198.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4126..4635
                     /locus_tag="IW687_001941"
     CDS             4126..4635
                     /locus_tag="IW687_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0951199.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4705..5244)
                     /locus_tag="IW687_001942"
     CDS             complement(4705..5244)
                     /locus_tag="IW687_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0951200.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5384..5995)
                     /gene="rpsD"
                     /locus_tag="IW687_001943"
     CDS             complement(5384..5995)
                     /gene="rpsD"
                     /locus_tag="IW687_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0951201.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6311..7108
                     /locus_tag="IW687_001944"
     CDS             6311..7108
                     /locus_tag="IW687_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0951202.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7136..7771
                     /locus_tag="IW687_001945"
     CDS             7136..7771
                     /locus_tag="IW687_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0951203.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7791..8564
                     /locus_tag="IW687_001946"
     CDS             7791..8564
                     /locus_tag="IW687_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0951204.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8972..9769
                     /locus_tag="IW687_001947"
     CDS             8972..9769
                     /locus_tag="IW687_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0951205.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            9747..10160
                     /locus_tag="IW687_001948"
     CDS             9747..10160
                     /locus_tag="IW687_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951206.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10144..12789
                     /gene="mutS"
                     /locus_tag="IW687_001949"
     CDS             10144..12789
                     /gene="mutS"
                     /locus_tag="IW687_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0951207.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12807..14915
                     /gene="mutL"
                     /locus_tag="IW687_001950"
     CDS             12807..14915
                     /gene="mutL"
                     /locus_tag="IW687_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0951208.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14937..15497
                     /locus_tag="IW687_001951"
     CDS             14937..15497
                     /locus_tag="IW687_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0951209.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5023 a         2749 c         3411 g         4362 t
ORIGIN      
        1 aaactttcag aaaaggtttg ttagtaaaat gaaagctata cgtaaggagt gttgtttgat
       61 gaaatttttt caagagtttt tcattattat tttcttttct gtattaggag aaggcatacg
      121 gatttttgct catttaccta ttcctggaag tatcttaggt attctttttt tatttttagc
      181 atttgaaatg catcttgttg atcctgaaaa aatcggagca actggtgatt ttttattgaa
      241 taatctagct attttatttg ttcctgctgg tgtaggttta ttggaatatt ttgatgatat
      301 tgcttcgata tggcctgttt tactgggagc agtcgtcgtg tgttccatcg tcacgatggt
      361 tgcagaggga aaaacggcag aaggtgtaga agctttgctg ggctatgtcc gtaagaaaaa
      421 agcaggaagg gtatctgtaa acgaaatgga ggaaaatcaa attgactgag ttttttgtga
      481 atccactatt tggcttaagt ttgactttag gagtgtatgt attatctgat aaatttttgc
      541 ggaaatataa actgcctgtt ttgaatcctc tcgtattatc gattgttgtt attatcttat
      601 ttttgcattt ttctgggatc agctataaag actataatat cgggggcaat tttttgacta
      661 tgctgattac tcctgcgaca gtggcacttg ccatccctct ttataaaaac ttccgtttac
      721 tgaaagagaa tttttatcct gtaatggctg cgattttagc aggaatagtc gcaaattgct
      781 tggttagtgt agggttcggt tacttcttct ctcttcataa agagatggtg atctcattac
      841 tacctaaatc tgtaacgacg gctatttctg tcgatttatc tcatacgatg ggcggggtga
      901 atgccgtgac acttgctatt gttgtctcta caggtatttt tggttcgctg atcgccactc
      961 atattttccg cctattcaag atcgaaagtc cagttgctcg cggtgtggcg ttaggaagta
     1021 cttctcatgc tatcggtaca gcaaaagcaa tcgagattgg cgaaatagaa ggaatcattt
     1081 ccggacttgc gatatgcgta aatgggatat tgaccgtttt gttgctgccg ttattctttc
     1141 agccttttgc tggcttgttt tgatgcgaaa accactattt tatgctacaa taaccaaaac
     1201 gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct tccgagtatc ttaaacaatt
     1261 tgataaagat ctatattata agattctcct cctagaaagt tttgaggatc aagcttggca
     1321 taccgctgca caattagcgc aagttgttca gctggatgcc cgcagcgtgt cgaaatacct
     1381 gaacgagctg tctaaaaact atcagcagtt tagcggtaag acacaccctt tgtttacaaa
     1441 aaatcaccgt tctggttata acttttatga tacgctcgat tcaattgaac atgagcgttt
     1501 tttgatctat ctcgtgcaaa gtacgctgaa attccagttg ctgcacgaca ttttttttga
     1561 agaattccat acaatgtacc aatttgcgca aaagcattac attagtgaat ctactgctca
     1621 tcgaaaaatc aatgaatgga aacagcaact gcaaacttac ggaattcggc tgcaacgagg
     1681 tacatatatc gctcaaggcg aagaagaaat cattcgactt tacttgcata tgacattctg
     1741 gcagttgttt cgagggaaaa tctggccatt tgaaacgatt tctcaaatgg acgtaaagaa
     1801 tatggcagaa catatcatgg ctttttttaa tgttcgtctg aacgagatca aaaaaagacg
     1861 attggaatac atgctaggtg cctttttttt aagaaaatca cagaagcatt atgttgtatt
     1921 gaatgagaaa aaaagacggc tgatttctga caatctgttg ttccagcgat tttgccaagt
     1981 catggagccg gtatttccta attattttca agtagaagac gagttaggcg cattatttct
     2041 tgtactgatg actcgtgaag aatattatag tgatcctaag atacggaaga aaatttttga
     2101 ttttcatcaa caagcaaaaa caccgccctt tacagccctt tcagaagcaa aagccgctct
     2161 ttctctttat caggaagagc aggggctgcc agcagaaaat ctaaccttcg aggcagaaaa
     2221 ctatttattc agcagtcatt ttttcgccta tctttttcct aatgcgaaag aaacgattga
     2281 tggcaatagt agtgatttta ttaatcattt agttattgaa aacaaggaat tgaaacaatg
     2341 gctcgtacat ttttttgaaa gtagacacaa acatcccaat catctggcat ttaaaaatca
     2401 tgcgtttttg atgggtcggt atctgactgt gtttaaaaca ttaggagcct ttactccaca
     2461 gcttccaaag atcacgatcc ttttaatgac agatttccca ttatttgaag agcaactgtt
     2521 agaggaggga ttacggacat ttttccgtaa tgagtatcag ctgatttttc tgccaacaga
     2581 ttatcggggg agagaagttg atcttctgat ttcgacaagt aaagtccacc gcaaaccatg
     2641 ggcggatctt gactatttta ttgtgaccga agaactaaaa ttaattgatt acatccaact
     2701 atctcaaaaa tttgaaatga ttcaaaaaaa aactattaaa acaataacaa agaaggagaa
     2761 aaaagatgac atttgaagaa attttaccag aattgaaaaa aggtgcaaag attattcgta
     2821 aaggatggag tggatttgaa ctttatgttg ttctcgtatc cgatgatgaa tttgatggct
     2881 gtccagtgac accttactta ttgatcaaaa caagtgacga aggtttttcc agcttcgctc
     2941 ctacagtttg tgatatttta gcagatgatt gggcgatcgt agaatgaaaa accagtatcc
     3001 agatctagct aaacaggttg tatttatcac aggagctgct tctggaattg gtgctgcgca
     3061 agcccgagcg tttcttgaac aagatgcttt tgtatttgga ttagatcaaa agttcggtaa
     3121 aatggaagaa ctctctttgc gatttcctga aacttttgcc tacgcactag gtgatgttcg
     3181 acaaatgaaa gaactccaac aagcggtatc gaaatgccag gatcgttttg gtgaagttac
     3241 gattttactg aatacatcgg ggatattgga tgcctacaaa ccattgatgg aaactgacga
     3301 atcgctgtgg gatcttattt atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat
     3361 catccttcct gatatgatcc gccgaaaaag cggaacaatc atcaacatgg cttcgattgc
     3421 gggtttggtt gctggcggag gtgggatcgc ttatacaagt gcgaaacatg caatcgtcgg
     3481 atttaccaaa caactcgctc tggattacgc aggacagggt atccgagtaa aaggaattgc
     3541 tccaggagcc atccaaacac caatgaatgc ggctgatttt gaagggaaag gtgagatggc
     3601 tgagtgggtc gcaaatgaaa caccagtcaa gcgatgggca cagccggaag aagtagctga
     3661 gctgacgtta tttcttgcct ctcctcaagc aagctacata caaggaacga tcatcccaat
     3721 cgatggcgga tggttgttaa aataaccttg tgtttttttc aagcttctgt atgataaaac
     3781 taaagagaga aacaacggtt gatgataaaa gaaagtcgat gactgttccc gacattcgat
     3841 gccgaacagc cagttcaaaa aattcttttt aataatcgtt aatattcgaa actaagcggc
     3901 ttttcacgac cttttgatat aaacggtgtt caaaagcttg caactgcggt aacaagtcca
     3961 gccaaatgaa aaatataagg agctattttt atttgactat tcttattact tgttgcaggg
     4021 cagctttttc actaccttct gcggcacttc agttggaatt ttcttcccat cctttggtcg
     4081 cagtcattac caagataaaa ttatcttgca aaggagaaat agaacatgga aaaaaataat
     4141 acccgttcag ccaaacgctg gacaacgaca gatgtgactc ggatggcgct agtcactggt
     4201 ttatatgtgg cagtgacgct ggttttgtct gtctttagct ttggagtgat tcagattcgt
     4261 ctctcggaga tgttcaacta tctgtccttg tataacaaaa gatacatcgt cgcggtaaca
     4321 gcgggagtag ctttggctaa tatggcttct cctcttggac tgatagatgt tgtagtagga
     4381 agcgtcagca cgctgcttgt attgcttatc aactataaaa tcactagtcg catcaaaaac
     4441 atgaaaatca aaatggctgt gacagcatgc gtatttgctt tttccatgtt tacagtagct
     4501 ggtcagctaa cgattttata tcaactacct tttttcttga attggtgggt gattgcttta
     4561 ggagaattgg catcaatgat tgtcggtggg ataattattt attggatcgg taaaaaagtg
     4621 gatttgtcaa aataaagatc tgtttaattt aaaatatggc agcgtggaag agactttgag
     4681 tcttcttcca tgctgctttt ttgattaatt tgtatgaaga aatcgattta gccgtttttt
     4741 catcaagata ccgatataag ctgctccagt tgccttgatt gcttccggca gtaaaaacgg
     4801 cagtagtcca ctggcaaatg cagttgtaaa agtaagatca ccacttattt tcagccagat
     4861 cgttccgcag atcaatgcag caagagagcc cattagattg gcaaagattg catataagta
     4921 atgagcatcc gttctttcga tcatccaacc agttatccac gtttgtaaga gaaatccaat
     4981 caaatatccg ccagttggcc caaataaaac agctatacct ccagtcatcc ctgaaaatac
     5041 aggaagacca atcaagccaa gtaaaagata aactacaaca gatatcaatc ctagttttct
     5101 tccgagaaaa gttgctgtta atccaatagc aaatgtttgc cccgtcaaag gaaccaagcc
     5161 aaaaggaatg gtcatctgag aaaggacaga aataatcgcg gcaaattccg cagcaagtat
     5221 taattctttc aatgttcggt tcataatgcc ctcttttcgt taacttaata tttttatttg
     5281 gttaacgaaa ttgtaaaaca aagtgattac tttgtaaata gaaaatataa aaaaagacca
     5341 gctatccaat agatagcggt ctttttcaag aaagcttttg aaattataag ctttggttgt
     5401 agtattcaac gacaagagct tcgtcgattt ctggatatag ttcgtcacgt tctggtaaac
     5461 gagtcaatga accttccaat ttttcagcgt caaagcttac gaatgctggg cgtccaacag
     5521 ttgcttctac agcttctttt actgttacga tgttttgtga tttttcacga actgagatca
     5581 cttgacccac ttctacgtgg tatgaaggga tatcaacgcg tttgccatca actaaaatgt
     5641 gaccatggtt aactaattga cgtgcttgac gacgagtcgt tgcaagtcct aaacggtaaa
     5701 caacattgtc taaacgttgt tctagtaaga tcatgaagtt aacaccatgt ttaccttctt
     5761 tgattttgct agcttttacg aacaagttac ggaattgacg ttcgttcatt ccgtacatgt
     5821 gacgaagttt ttgtttttca gttaattgca tgccgtattc tgattgtttg ccacggctgt
     5881 taggtccatg ttgacctggt gcgtaagggc gacgtgctaa ttcttttcct gtgcctgaca
     5941 atgagatgcc taagcgacga gaaattttcc atgatggtcc tgtataacga gacattaaaa
     6001 tttcctccaa tgaatataag tttttggagt aaaataatcc gttgaaaaat tcataatcat
     6061 atagtttgtt cttcaatctt cacctttgca gccgcggtta cacaatcgaa cccgagacgg
     6121 ggcaacaaac tagggatgag ttattatttt tctgctgcat tattttacac aaactacagt
     6181 atatgataga tcatccacga atgtcaagat aattagtaca taaaaacgct ttacaaacct
     6241 aggctgtctt tataaaatga gagggcatgt gcaaatggaa aaaagcgaag gaagtttaag
     6301 gagaaaatca atgaatccgg tatcaccatt atttgagaaa atcgatgctt caattgaaaa
     6361 gaaggcgaat ctttttgaaa acagcttttc acgttacgct gttcgagcgg ttctcgcctg
     6421 tttgtttttg actttaggaa cggctgtggc atttgcaatc gcgatgaagg gggaagatat
     6481 cgcgcatggt ctaggaaaga ttttatacgc atttatgttc agctggggtc tggtcatgat
     6541 tctatacatg aacgctgaat taggaacatc gaatatgctt tatatgaccg tgggtgtttt
     6601 tcggcagaga cttgctttaa gtaaggcagc caagattcta tttgcctgta tttttttcaa
     6661 tatgattggc ggtatattat gtggatttct tatttctatg actacgccat ttcaagattt
     6721 aacgttggac aactttatgt tagaatcaat agcagtaaaa ctagcaaaac ctgcagcaac
     6781 gattttggtg gaagggatgt ttgcgaatat cgttgtcaat accgctgtac tgatcagcat
     6841 gcggatgaaa gaggatgccg ggaaagtgat cacagtcgta ttcatcattt tcatttttgc
     6901 ttttttagga tatgaacacg tgatagcgaa ttttcctgcc tttacgcttg cttactttgc
     6961 ttctcaagga cagacggatg gaatgacagt cagcaatgtg ttacataatt tgttttttgc
     7021 tttggcagga aattatattg gtggcggttt agtaatggga ctgggatatg cttggctgaa
     7081 tcagtcaaaa tcatcctatg tggactaaaa agtcgttcat taaaggggaa gtgccatgga
     7141 agttttagtt tttccaaaaa atgaggaaaa agcgttacaa atggaaaagt acatgaaaaa
     7201 tcttttttcc tttgctggag tacctaagcc ggaacgcgct cttttacaac aaccgttact
     7261 ggatgagagc aaaaatttat ctatttctga agttttagct tttgtttatt attattatga
     7321 acaaatcgaa agagagtatc agtatgtagc gatcgattta gtccagaaaa acatcaaacg
     7381 gcttacattt gaagacttag tcgtattgtt gccactagtt cagaaaaaag aatggtggga
     7441 cagcatcgac tcttggagaa aagtatacag cacatggtgt aaagcctacc cagagcagcg
     7501 tgaagaagtc tttcaattgt ttgaaaaaaa ggaagatttt tggctacgtc gaatagcaat
     7561 cacgttgcaa ttaggttata aacagcaaac agacacagga ctattgatcc gagcaataga
     7621 cgaagatcgt caaacaaagg aattctttat ccaaaaagca attggttggg cattacgcga
     7681 ttacagtaag accgatgccg attgggtaaa aagacagctg acaacagatt tgagtaagtt
     7741 agctgttcga gaggcaagta agtatttata agaaagcgag acgacaagag atgagacgca
     7801 aattatttgc ttttgatatt gatggaacgt tgttagattc aaacaaacaa gctttagata
     7861 gcacgagaga agcattggaa aaattacgca aagccggtca tctggtcacg attgctacag
     7921 ggcggagccg atttcatgcg caagagatca ttcgcgatct tgattttacg aactacattt
     7981 tatgtaatgg agcggccggt tttttagacc atgaacaagt atataagaat ttactagatc
     8041 gcgaccagtt ggatgcgttc gttcaagaag cgcatcatga gcagatcgat acagcttttg
     8101 tcagcttaga caatatcaag cgttcttctt ccaatgatgt aacggtgatg gaagaagcga
     8161 tgcattcttt cggtgctgtc ttgcctgaat tagatcagtt ttttgcagag gaacaagaaa
     8221 tataccaagc gttagcattt ttcgatcaaa catatgatgg gaagttctct tcatatgata
     8281 aaattcgatt tgtccgctgg catgagaaca gtgtagatgt cgtacctaaa aatggttcta
     8341 aagcagcgac aattctgcac ttagcagaac aagtaggcat ccctcatgaa gacatcatca
     8401 gctttggcga tggacaaaac gaccgtgaaa tgttgcgcat gtcaggaatt gggatagcta
     8461 tggggaatgc tgtgccagaa gtacaagcag aagctaagat ggtcactgat acaaacgatc
     8521 aggatggaat ttggaaggca ttgaaagagt taagcgtgat ttaaaagagg ttgtgaaaaa
     8581 gctgttctgc atcaagtaat aagcccagcc aaacaaaaat agctcctcct atttttcgtt
     8641 tttctgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggtcgtga
     8701 cagaagtggt cagtttcaag aaataagaag ggatttacga aaattgcttc tcaaattttt
     8761 gtaaaattac ggcttatttc tgaagaagta gatgaaacaa cgtttattcg ggaaatggga
     8821 ctgtgacaag atgtttgtca tagtcccatt tttttatttt atttttcaaa actaagaaat
     8881 accaggaaag agttcctttt gtttgacctt ttctttctta ttgaaaacaa ctataatata
     8941 gagtgatgtt aaaagagaag aggaatttag tgtgcgggta ttgtttattg gtgatgtagt
     9001 aggttcaatg ggacgagaga tgattactga atatcttcct agattgaaga agaagtaccg
     9061 tccgcaagta acgattgtaa atggtgagaa tgccgcttct ggacgtggaa tcacagaaaa
     9121 aatctataaa aaatttttgc aagacggcgt agatgtcgtg acgatgggga atcatacgtg
     9181 ggacaaccgc ggaatctttg agtttgtaaa tgaagctaaa aagatggttc gtccggcaaa
     9241 ttttccggaa gggacgcctg gacaagggat ggtctttgtc aaagtgaacc aagtcgaact
     9301 ggcagtgatc aacatgcagg ctcgttcttt tatggtggac ttggatgatc cttttcgtaa
     9361 aatgaatgag ctggtagaag aagccagaaa acgtacgccg atcatttttg tagattttca
     9421 tggagaaaca acaagtgaaa aacaagcaat gggctggttt ttagatggga aagtttcagc
     9481 agtggtcggt acacatacac acgttcagac aaatgatgcg cgaattttgc caagaggaac
     9541 agcgtatctg acagacgttg ggatgacagg tccctatgat ggtattttgg gtatgcgcag
     9601 agaacctgta atcgaaaagt ttctgaccgc attaccaaaa cgttttgaag ttgtggaaca
     9661 aggccgatcg attttaagcg gctgtatctt ggaattggat gatacgacag gtcatgcaaa
     9721 aaaaatccag ctgattcaga tcaacgatga tcgaccgttc atggagtaga aagagaggca
     9781 gttttttgca gcaggagccg gaaattcaaa aagaagtaag gaaactgaca aaacttttaa
     9841 gagagaatga aacgatcatt cgctacaaag aacttgaaga aaagatccag caaaaccagt
     9901 atttagctga gttgagagaa aaaatcaaac aagcgcaaaa agatgctgtt cattttgccc
     9961 actatgacaa accagcagca gaacaagaag caatcaaaca agcggatcaa ttcatgcaag
    10021 aatttgatca gcatccgctt gttgtagctt atagaaaaca gctgttggaa gcagatgatc
    10081 tgcttcatca tttaacaact atgatacaag aagaaatcaa tggacaaata gaggaggaaa
    10141 aacatgcctc aaaaaactaa aaatactcct atgatggaac aatatctaag catcaaggcc
    10201 caataccaag atgctttttt gttctatcgt ttaggagact tttatgaact attttacgaa
    10261 gatgcgatca aagcgtcaca gcttttggaa ttaacgttga cgagcagaaa tcgaaatgca
    10321 gaagaaccta ttcctatgtg cggtgtacct caccatgcag cacaaggata tatcgatgcc
    10381 ttgattgaaa aaggctataa agtagcgatt tgcgagcagg tagaagatcc aaagacgact
    10441 aaagggatgg tcaagcgcga agtgatccag ctagtcactc ctggtacagt catggacagc
    10501 aaaggattgt cggcaaaaga caacaatttt ttaacagctg tagttagtca aggaaatgaa
    10561 tttggctttg cttatgccga tttaagtacg ggagaattga agacggctcg gttgcatgat
    10621 gaagaagctg ttttaaatga agcctctgca ttgcagacaa aagaactcgt attaggcagt
    10681 gagatcactg atacccttcg tgaacagcta agcgggcgtt tagggctagt cttttctcaa
    10741 caaaatgaga tggaagaaaa cgcggaattt agctttttga caagcgaact agtcgatccg
    10801 ctgaaaaaag aagcaaccgg caaactattg acttatttat cggtgacaca aaagcggagt
    10861 ttagctcata tccaaaaagc agtagaatac caaccagatc attttttgaa aatggattat
    10921 tattcaaaat tcaacctaga attaagccaa tctattcgga ctgggaaaaa gcacggcact
    10981 cttctatggt tgttagatga aacaaaaaca gcaatgggtg gccgtttact aaaacaatgg
    11041 ctagaccgtc cgcttatcca ggcaaaacaa attaaagcaa gacaagaaat ggtcgcttct
    11101 ttgttagaca gctattttga acgtatcgat ttgcagtcag cattaacgaa agtctatgat
    11161 ctggaacgcc tagcaggtcg agtagctttc ggtaatgtca atgggcgaga cttgatccag
    11221 ctgaaaaatt ctttagaaca agtgccaatg atcagagggt tgattgaagg aatcgatcaa
    11281 ggtcaatggc aaagtctgct ttcagaaatg cagccgatgg accatctcgt ccaattgatc
    11341 gaccaagcta tccaagatga tccgccgtta caaataacgg aaggaaacat catcaaagat
    11401 ggattcaatg agcagctcga tatgtaccgt tctgctatga gaaacggaaa gaaatggttg
    11461 gcagaacttg aagcaaaaga gcgtcaagaa acaggaatca aaaacttaaa aatcggattt
    11521 aaccgcgttt tcggttatta tatagaaatc acaaaagcaa atcttggaaa tgcagaactg
    11581 gagaagtatg aacgaaaaca aactttagca aatgcagagc gtttcatcac accagaacta
    11641 aaagaattgg agacacaaat cctcgaagca gaagaaaaat ctgttgattt agaatatcag
    11701 ttgtttttag ctgtgagaga agaagtcaaa aaggctatcc agccgctaca agtacttgca
    11761 aaagcaatca gtgcagtaga tgtgctacaa agttttgcaa cgatcagcga acgctatcag
    11821 tatgttcggc cagaacttgt cagtgacaag catcagcttc tgatcaaaga cggacgacat
    11881 cctgtagtag aaaaagtatt aggacatcag gaatatatcc ctaacagcgt agaaatggca
    11941 gaagacgaaa tgatcttgct gattacagga ccgaatatgt ctggtaaaag tacgtatatg
    12001 cgacagttgg cattaactgt tctgatggcg caaatgggct gtttcgttcc ggcaaaacaa
    12061 gcagttttac ctatttttga tcggattttt acccgaattg gtgcaagtga cgacttaatt
    12121 gctggacaaa gtacttttat ggtagagatg atggaagcca atcaagccct gcgttatgct
    12181 acgccaaaca gtttgatttt gtttgacgaa ctagggcgag gaacagcaac ctatgacgga
    12241 atggcgttgg cccaagcaat catcgaatat atccatcgtc atgtacaagc aaaaacactc
    12301 ttttctactc attatcatga actgacggta cttgagaaag aacttccgca gcttaaaaat
    12361 gtccatgttg gagccgttga aaaagacgga gaagttgtct tcttgcataa aatgatggat
    12421 ggacctgcgg ataaaagcta cggcatacat gtggcaaaga tcgcaggatt gccttccaat
    12481 ctactagaac gagcagcaga tatcctgcat acgctagaaa atggcgataa cggacaccat
    12541 actgttccag ctgaagtaga agagaaaact aaaacagcca aacaggagca aataaaacca
    12601 cctgcagaaa aagtaaatca agaccatata caagaccgtg taaaagaaga aaaccaacag
    12661 ctttctttat tcagtgaatt atccgaaaat gaaacagaag tcattgaatc actgaagcaa
    12721 ataaatttac tagaaatgac gccaatggat gcactaaaca cattatatga attacaaaaa
    12781 caactgtagg aagaaggcgg atatcgatgg ccaaaatcca agaattatct gaacgtttag
    12841 ctaatcagat cgctgcggga gaagtagtgg agcgaccagc ttccgttgtg aaagaattag
    12901 tggaaaatgc aattgacgcc ggcagtactc agattgatat cttagttgaa gaagcgggac
    12961 tgcggacgat ccaagtgatt gataacggag aaggcatttt ggcagacgac gtagagaatg
    13021 ccttcaagcg gcatgcgaca agtaagatcc ataatcgcga cgatcttttt cgcatacgaa
    13081 cgctgggctt tcgaggggaa gctttgccaa gtattgcctc tgtttctgaa atgactctgg
    13141 aaacggcgaa tcaggaagaa aaacaaggca cctatctgtc gctgaaaggc ggagaagtta
    13201 ttgagcatcg accagcacca ctgcgtcaag gaacgaagat taccgtcagc aatctgtttt
    13261 tcaatacgcc agcccgcttg aaatacgtga aaacactgca cacagaatta gcgaacatcg
    13321 gggacatcgt caatcgactg gcattgagtc atccatcgat tgcttttcgt ttagtacatg
    13381 atggaaacaa aatgctggca actgctggaa atggcgatct gaaacaaaca attgcgggaa
    13441 tctacggatt atctaccgca aaaaaaatgc tgaaaatcga aggaaaagat ctagattttg
    13501 agttatttgg ctatgtttca ttgcctgagg tgactcgtgc cagtaggaat tacttatcga
    13561 cgatcatcaa tggacgtttt atcaagaact tttccttgaa taaagcaatc gttgcaggtt
    13621 atgggtcaaa attaatggtc ggtcgtttcc ctattgctgt attggaaatc aaaatggacc
    13681 cgcttttagt agatgtaaat gttcatccga cgaagcagga agtccggctg tcgaaggaaa
    13741 aagaactaac agagctaatt agtaacgcca ttcaaaaagc attgtctcaa gaaaatctga
    13801 ttccaagtgc agcggataat ctacggttta agaaaaaagt acctgatcag cctaaaacag
    13861 aacaaatcga aatcgatttg tcctacgctt ttgaagaaga aaaacctaaa aagccaagta
    13921 gtttgaattt tgatcgagag acaggtacct ttttcgtaga agaaccaaag aacacatttg
    13981 ttgacagaat tgttgataag tctgtggaaa atgttgaaaa ctattctggt ttttctgaag
    14041 aaaacaccac ttatccagaa aaaacagcga aaacggtgga taagtttggt gaaaatgttg
    14101 ataactttgt agaaaaaaca gtggaaaaat ctgaaaagaa agaatttccc gttgtggata
    14161 acatggagaa aacaaatgaa gaacaagcag ataaatccgt agaagaggat gaaaagattt
    14221 acgaggaaga attgacactc catcctgaat ttgatttcac tcaaggaaac gacaaaacat
    14281 tagaacaagc catcaagaaa ttagaaacgg aaaaaccagt acaacgtttt cctcagttgg
    14341 aatattttgg acagatgcac ggaacgtatc tttttgccca aagtagcgat gggttgtaca
    14401 tcattgatca gcatgctgcc caagagcgca ttaagtatga atatttccgg gaaaaaattg
    14461 gggaagtatc tgatgatctg caagaattgc ttgttccatt tgtgttagac tatccaaaca
    14521 gtgatgcttt gaaattaaaa gagcaaaaac acttgctaga agatgcaggg atttatttag
    14581 aagaatttgg tcaaaacagc tttattgtac gggcccatcc tacttggtat cccaatggag
    14641 aagaagaatc gattattcgg gagatgatcg atatgctgct gacaactgga agtgtgagtg
    14701 taaaaaaatt ccgtgaagca acagcaatca tgatgagttg caaacgatcg attaaagcca
    14761 accattattt gaatgaagca caagcacgtg tgctgctgaa ggatctggca cgatgcgaaa
    14821 atcctttcaa ttgtccccat ggccgtccag tattgattca ctttaccaat tcggatatgg
    14881 aaaggatgtt caaacgaatc caagacccgc attaagtcaa agagaagagg gaaaacatga
    14941 agattgtatt agcatcccaa tcaccaagaa gaaaagagtt attggctcat atcgtttcag
    15001 aattcgagat tgaacctgct gatatcgatg aaacgccatt tccagacgaa cagcccgtcg
    15061 attatgttcg tagaatggct gaagaaaaag cacgaattgt ctgggaacaa gatgaaaaag
    15121 aagaccattt agtgattgcc agtgacacga ctgttgttct caatcaagag atcatgggta
    15181 aaccagaaga tctggctgag gctgaattca tgctgaaaaa actaagcggg gaaacacata
    15241 ctgtatatac agctgttgtt ttaaaaacaa aggaacgaga agaacggata ctagcagaag
    15301 cacatgtcac tttttatcct ttgacagacg aagagatcaa acgctacttg gagacggggg
    15361 attatgcgga taaggcaggg gcatacggca tccaaaatgc tgcgagtgta ttcgtcaaag
    15421 aaatttctgg agattattac agtatcgtag gatttccgat tggtgctgtc aatcaggcac
    15481 tgaaaacatt catttaaaca aaaaagcgga ctatctgatt ttcgattgaa aatccatgat
    15541 agccc
//
LOCUS       DACELF010000064        14994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.067, whole genome shotgun sequence.
ACCESSION   DACELF010000064 DACELF010000000
VERSION     DACELF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.067"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW687_001952"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW687_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0951210.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="IW687_001953"
     CDS             complement(321..890)
                     /locus_tag="IW687_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0951211.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="IW687_001954"
     CDS             1076..1978
                     /locus_tag="IW687_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0951212.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW687_001955"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW687_001955"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0951213.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW687_001956"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW687_001956"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0951214.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW687_001957"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW687_001957"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0951215.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="IW687_001958"
     CDS             8546..8731
                     /locus_tag="IW687_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0951216.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="IW687_001959"
     CDS             complement(8793..9989)
                     /locus_tag="IW687_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0951217.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="IW687_001960"
     CDS             complement(10159..11595)
                     /locus_tag="IW687_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0951218.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="IW687_001961"
     CDS             11888..12697
                     /locus_tag="IW687_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0951219.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..14880)
                     /locus_tag="IW687_001962"
     CDS             complement(12883..14880)
                     /locus_tag="IW687_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0951220.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2660 g         4779 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaa
//
LOCUS       DACELF010000065        14890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.068, whole genome shotgun sequence.
ACCESSION   DACELF010000065 DACELF010000000
VERSION     DACELF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.068"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW687_001963"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IW687_001963"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0951221.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1197..2216)
                     /locus_tag="IW687_001964"
     CDS             complement(1197..2216)
                     /locus_tag="IW687_001964"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0951222.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2849..3853)
                     /locus_tag="IW687_001965"
     CDS             complement(2849..3853)
                     /locus_tag="IW687_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0951223.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3866..4915)
                     /locus_tag="IW687_001966"
     CDS             complement(3866..4915)
                     /locus_tag="IW687_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0951224.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4932..5753)
                     /locus_tag="IW687_001967"
     CDS             complement(4932..5753)
                     /locus_tag="IW687_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0951225.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5740..6519)
                     /locus_tag="IW687_001968"
     CDS             complement(5740..6519)
                     /locus_tag="IW687_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0951226.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6536..7006)
                     /locus_tag="IW687_001969"
     CDS             complement(6536..7006)
                     /locus_tag="IW687_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0951227.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7018..7437)
                     /locus_tag="IW687_001970"
     CDS             complement(7018..7437)
                     /locus_tag="IW687_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0951228.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7518..10286)
                     /locus_tag="IW687_001971"
     CDS             complement(7518..10286)
                     /locus_tag="IW687_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0951229.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10486..10710
                     /locus_tag="IW687_001972"
     CDS             10486..10710
                     /locus_tag="IW687_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951230.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11489..13204
                     /locus_tag="IW687_001973"
     CDS             11489..13204
                     /locus_tag="IW687_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951231.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13207..>14890
                     /locus_tag="IW687_001974"
     CDS             13207..>14890
                     /locus_tag="IW687_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951232.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIV"
BASE COUNT         4323 a         3071 c         2559 g         4937 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa
     1201 cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt
     1261 ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca
     1321 cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt
     1381 cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc
     1441 caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg
     1501 ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct
     1561 cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca
     1621 ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt
     1681 gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga
     1741 tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta
     1801 ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact
     1861 tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc
     1921 ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca
     1981 acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata tccgataatc
     2041 gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca
     2101 tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt
     2161 tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat
     2221 attcctccaa ataaataatt gataatttcc attatagtat acatttctta aagttgggac
     2281 taatttccat tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc
     2341 aatgatatgt tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct
     2401 aagaggaagc agttttgaca atgttactac cagcattact attaggaggt taggaaactt
     2461 ctcctagtga gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc
     2521 aggtcctgca acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt
     2581 caacattctg gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta
     2641 ctacggatcc atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca
     2701 catgtcccat taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata
     2761 acgtaattga tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat
     2821 tttttatatt ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta
     2881 tctctaatgg atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat
     2941 gcatcaataa gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat
     3001 agtcttttgt gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac
     3061 gttcagataa cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt
     3121 ctacgatctc taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc
     3181 agctttgttc ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg
     3241 ctccaataaa ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg
     3301 catctgcttt tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat
     3361 agtattctga ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa
     3421 aaaatttgat ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag
     3481 acaacttact gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt
     3541 gttctactgc tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag
     3601 aatcttttgt taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga
     3661 caggcaagtt gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc
     3721 caccgattcc tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt
     3781 cttctacttg cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat
     3841 tgaatttcag catgatttcc acctcttata atttactttt caagactgta tcaaagtctt
     3901 caaaaatctt tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt
     3961 gaaacggaac gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa
     4021 gtgttttctc gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac
     4081 tcatatatct ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga
     4141 atagcaaatg cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa
     4201 atccttgtga cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc
     4261 cccagtttgg accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat
     4321 atccatcaaa aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc
     4381 gcaactgttg catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa
     4441 ttcctatccc taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc
     4501 ctactgattc taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac
     4561 tttgcacgtc atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg
     4621 cttcaacagt agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag
     4681 gagaaagttt tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac
     4741 cataagattc caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta
     4801 agatcagtaa attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca
     4861 agatcgtact tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct
     4921 ctgctccttt tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg
     4981 atcaagatcg ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct
     5041 ccaagacata agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt
     5101 tctcctgaac cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca
     5161 acgaccatca gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc
     5221 tggatctttt tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga
     5281 aacagaatgt gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct
     5341 tgcatggcta aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa
     5401 gaatctccta accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc
     5461 acatcaaatt caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc
     5521 agtgtaacga tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc
     5581 atttcttctt tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc
     5641 atattcatct gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg
     5701 tgatttagct cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc
     5761 tacctcccct gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac
     5821 aactgctaaa atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc
     5881 tatagcaaaa cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag
     5941 catcgcaaaa ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa
     6001 gtcaggaatc atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat
     6061 taccacaaca ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac
     6121 tcctttataa tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa
     6181 aagccctaag taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa
     6241 aagcgcggtt tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc
     6301 ggtcacaaca tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc
     6361 taaagttgcg cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag
     6421 ccccgtcaca atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac
     6481 aaatgcaaca attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca
     6541 aaacttctct tacatccact tttttatctg ctggtacttg acggatttct acttctattt
     6601 gtttgtcaat taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc
     6661 cgatgctgcg agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg
     6721 gatacgcttc tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat
     6781 atttatctgt caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta
     6841 gtttgactcc ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta
     6901 catcatcatt agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta
     6961 cttgtccgtg taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta
     7021 aaattcgtct tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt
     7081 tgaatcagtt agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga
     7141 tactagctca atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt
     7201 gatatattgt aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat
     7261 ttctttatct ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc
     7321 atcagtatag caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga
     7381 atctaatatc cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt
     7441 ttcatctcct cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg
     7501 caacaacttg cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc
     7561 aaaatatcat agatataggc aatctcagaa ttggggatca tgacactata atgctcttca
     7621 atgacactaa acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc
     7681 tctaatcctt gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc
     7741 acatggacat acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct
     7801 aattctctca taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg
     7861 gaatccagta ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc
     7921 tctgaagcat tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag
     7981 atgaggtctt ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc
     8041 attcctacga cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc
     8101 tgatattcat atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt
     8161 tcacttaagt ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt
     8221 ttgttttcct ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct
     8281 gctatctctt ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt
     8341 gtcgtcacat tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg
     8401 atttctttca gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt
     8461 tcgcaataat caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca
     8521 aatgattcaa atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca
     8581 gttgcataac cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc
     8641 gcttttttta gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat
     8701 ttgatcttac ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga
     8761 gaaacttgtt gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat
     8821 ttgatagtgc ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat
     8881 ttgatttgat aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa
     8941 accagcagct cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga
     9001 atcacttctt gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa
     9061 tcaattagga tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc
     9121 attaattgct ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta
     9181 gtaataggct gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc
     9241 tgttcacgct gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct
     9301 cctatgtttc cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac
     9361 aaagggctcc ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt
     9421 tcattacgac tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg
     9481 aaagttgtta agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg
     9541 atccggcgtg acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg
     9601 aacagttttt cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca
     9661 ccatctgctg cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta
     9721 acatgtccaa acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta
     9781 aaagtgatga atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc
     9841 aaattaacta aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg
     9901 ccatctggat agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg
     9961 tatccgatca taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg
    10021 agatcatcga tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct
    10081 tctttgtgga aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt
    10141 aactggttca aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt
    10201 tgtgctgtaa agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag
    10261 taacataaaa tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat
    10321 tcattttgtc aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca
    10381 gcctacttat cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata
    10441 ctggaatttc aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa
    10501 tataaaaaca ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc
    10561 gctgtagttg tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt
    10621 ggcgtagata cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt
    10681 gcgttctata aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt
    10741 tttagctatt acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa
    10801 aacgagttat gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga
    10861 ttacgtacac cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat
    10921 gttcctacct tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg
    10981 atgtcgggag gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt
    11041 tctccactat tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct
    11101 cattttcata ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa
    11161 aattacgtag caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc
    11221 tataaacatt tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa
    11281 atagacgttc ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa
    11341 aatagtctat tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt
    11401 gttctctttt cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg
    11461 tggacaaata aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta
    11521 tcgtaagcaa atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt
    11581 cctgccttta atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa
    11641 aatcattgaa atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat
    11701 catttgccaa tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca
    11761 gctaatcaaa aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac
    11821 cttgatcacc cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat
    11881 tcgtttattg atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat
    11941 cgactgggaa gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt
    12001 ttttatcatc aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct
    12061 caatgaacac gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac
    12121 aaaaaaagaa acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg
    12181 tgtttccaat atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat
    12241 catctgtttg ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca
    12301 agttcttgct ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa
    12361 cctcgtcgtg atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga
    12421 cacagaaaat tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc
    12481 aattatcgct tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca
    12541 aaatattaca tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag
    12601 cgggaaaacg actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt
    12661 tataatggac ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc
    12721 tcttgttccc caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg
    12781 taagaaagac gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga
    12841 atttgtccgt cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt
    12901 ctctggcgga caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt
    12961 actgatttta gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa
    13021 ggcgctacag acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat
    13081 tcaagaagca catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca
    13141 tgaagaatta cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga
    13201 atagttatga aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt
    13261 actgcctatg caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca
    13321 acagttgtga tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag
    13381 gtccgcttca atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac
    13441 gtagtcagtc aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca
    13501 aaattaagaa aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa
    13561 aactcccacg gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca
    13621 atttctgcgg ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt
    13681 tttatgatgc gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt
    13741 ttccttgtga gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg
    13801 attattgggg atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa
    13861 gcttttcagc aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat
    13921 gaaaaaggac aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta
    13981 gatcaccttg cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt
    14041 tcgtcaatca ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa
    14101 ccatttatcg aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa
    14161 cgagcatttg atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc
    14221 ttggaaccca aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac
    14281 cccaagcagc gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct
    14341 atagtaggaa aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat
    14401 gacacaacaa aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat
    14461 acattacgta aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg
    14521 cgagaaaatc ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta
    14581 aaagcctctt atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt
    14641 ggcggtcaag ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg
    14701 atgatcagta atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg
    14761 acagagaaaa aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg
    14821 attgcccatc gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga
    14881 gctattgtcg
//
LOCUS       DACELF010000066        14626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.069, whole genome shotgun sequence.
ACCESSION   DACELF010000066 DACELF010000000
VERSION     DACELF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.069"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..603)
                     /locus_tag="IW687_001975"
     CDS             complement(<1..603)
                     /locus_tag="IW687_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0951233.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETV"
     gene            767..1669
                     /gene="gnd"
                     /locus_tag="IW687_001976"
     CDS             767..1669
                     /gene="gnd"
                     /locus_tag="IW687_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0951234.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHQKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            1671..2672
                     /locus_tag="IW687_001977"
     CDS             1671..2672
                     /locus_tag="IW687_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0951235.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            2669..2953
                     /locus_tag="IW687_001978"
     CDS             2669..2953
                     /locus_tag="IW687_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0951236.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2986..3783
                     /locus_tag="IW687_001979"
     CDS             2986..3783
                     /locus_tag="IW687_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0951237.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3797..4630
                     /locus_tag="IW687_001980"
     CDS             3797..4630
                     /locus_tag="IW687_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0951238.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            4648..5505
                     /locus_tag="IW687_001981"
     CDS             4648..5505
                     /locus_tag="IW687_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0951239.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5527..6036
                     /locus_tag="IW687_001982"
     CDS             5527..6036
                     /locus_tag="IW687_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0951240.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6073..6474
                     /locus_tag="IW687_001983"
     CDS             6073..6474
                     /locus_tag="IW687_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951241.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            6586..6939
                     /locus_tag="IW687_001984"
     CDS             6586..6939
                     /locus_tag="IW687_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951242.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6973..7416
                     /locus_tag="IW687_001985"
     CDS             6973..7416
                     /locus_tag="IW687_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0951243.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7433..8518
                     /gene="uxuA"
                     /locus_tag="IW687_001986"
     CDS             7433..8518
                     /gene="uxuA"
                     /locus_tag="IW687_001986"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0951244.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8511..9224
                     /locus_tag="IW687_001987"
     CDS             8511..9224
                     /locus_tag="IW687_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0951245.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            9553..10854
                     /locus_tag="IW687_001988"
     CDS             9553..10854
                     /locus_tag="IW687_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0951246.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            11477..11677
                     /locus_tag="IW687_001989"
     CDS             11477..11677
                     /locus_tag="IW687_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0951247.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            12245..12808
                     /locus_tag="IW687_001990"
     CDS             12245..12808
                     /locus_tag="IW687_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951248.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(12869..13213)
                     /locus_tag="IW687_001991"
     CDS             complement(12869..13213)
                     /locus_tag="IW687_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951249.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(13310..13555)
                     /locus_tag="IW687_001992"
     CDS             complement(13310..13555)
                     /locus_tag="IW687_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951250.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(13625..13879)
                     /locus_tag="IW687_001993"
     CDS             complement(13625..13879)
                     /locus_tag="IW687_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951251.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(14043..14366)
                     /locus_tag="IW687_001994"
     CDS             complement(14043..14366)
                     /locus_tag="IW687_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951252.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            14553..14626
                     /locus_tag="IW687_001995"
     tRNA            14553..14626
                     /locus_tag="IW687_001995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14587..14589,aa:Ile,seq:cat)
BASE COUNT         4989 a         2363 c         3024 g         4250 t
ORIGIN      
        1 tacggtttca gcaatatgga ttgtttcttt tgattgccct gtgtgagaaa tcaaaacagc
       61 acagtctttt tccgtcaata aagaagcttc catcaattga atatggtaat ccgtgctgtg
      121 agaagttgcg ataggggaac gcaaaaattt atgataagcg tcagttgcta caatctctga
      181 accgccaaca ccgaaaaaat agacacgttt agattcattg ataatagtag ccgctttttt
      241 tagatccttt tgttgtaaca acttttttgt gtcatttaaa gttgaaatac tagactcgaa
      301 caccttttgc gccatagtga gctcagtatc tgttttctga atattttcgt ggatagaaat
      361 agcagataga tcattttctt ggatcaacat ggccatttta aaatctttaa atccattata
      421 accaagtttt tttgtaaatt gaaaaaaagt agagtcagca atgcccagtt cacttgctaa
      481 attgctaata ggactatgac ttactttgct tggattttct aaaatataat cggcgatagc
      541 ctgttctttt gggctgaagt cgttgtacat tgttttgatt tgtagtctta tggagacgct
      601 catgagaatt cctcattttc ataataagat tatcattttt tccgttctca ttaatgttaa
      661 agatacaagg agagtaggaa aaaatcaaac aaaaaatatt tttgtaaaat ctatttacaa
      721 aaaaattttt ttgtataatg agggtataag aaatggaggc gctatcatgg aaattggtgt
      781 aattggtttg ggaaaaatgg gattgaattt agctttaaac ttaaaagagc accatcaaaa
      841 agtacaagga ctcgacattt caagtacagc tgttagtcag gcaagagaaa atgacattcc
      901 tgcttatcaa gagatctctg aatttctagc tacttttgag cagaaaaaaa tcatctggtt
      961 aatgcttcct gctggagaga taaccgaaaa catgttaaga aaactttttc caaaactcgg
     1021 accaggagac attattattg aaggcggaaa ctcttattat aaagattcaa tccgtcgagc
     1081 agaagagttt cagaaaaatg aaatcggtta cttggattgt gggacttctg gaggaatcga
     1141 aggagcacga catcaagcct gcttaatgat tggaggagat aaagaaactt ttctatccgt
     1201 ggaacaatta ttcaaagatg tagcaattga aagaggatat ctgtatgctg gtgcatccgg
     1261 aagcggacac ttcttgaaaa tgatccataa tggaatcgaa tatggaatga tgcaagcaat
     1321 cggtgaagga ttccaacttg tacaaaaaag tgagtatgct tttgacttgg aacaagtcgc
     1381 acgtgtttgg aatcatggtt cggtgattcg cggctggctg atggaaatca tggaagatca
     1441 gttaaacgaa catcccaatt taagtaatta tcggggaatc gtttctgcat caggtgaagc
     1501 aaaatggacg gttgaaacag cgctagaaat ggctgttcca gttccagtaa ttgcaactag
     1561 tttgttcatg aggaatcttt cacaggaaga agatagtttt tcagcaaaag tagtttcagc
     1621 actaagaaat gggtttggcg gacatgaaat agttgaaaag ggtgagtaaa atgccagatt
     1681 atcatatagc aatcgttaat tccagcagtt tcggaagagt ctttccacac cacttggaaa
     1741 gattaaagtc agttggagac gttcatttct ttactgttga tcaagagatt ccaggaaaag
     1801 aactagcgga gttacttaaa ggattcaata tcattattgc aagtgttacg ccattttttc
     1861 gacgagattt ttttgaaaac aaagatgaac tgctattgat ttcccgacat ggtattggtt
     1921 acaacaatat tgatttaaaa gcagcaaaag aacaccagac aatcgtatct atcatacctg
     1981 ctttagttga acgaaatgct gtagcagaaa ataatataac taatttgttg tccatgcttc
     2041 gacgcacgac acaagcacaa gcaagagtca aggacgatcg atgggaagat cgggcggagt
     2101 tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat tggtgcagga aataccggaa
     2161 gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga tgttctagct tatgatcctt
     2221 acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa agtagaactt caagaattac
     2281 tggaacaatc agatatcatt tgtttatgtg cgaatttgac agaagagaac tatcacatga
     2341 tttctgataa agaagtcgat cagatgaaag aaaatgttta tctatctaat agtgcaagag
     2401 gcgctctagt tgatgaagaa gcgatgatta atggtctgaa aagcggaaag atcgcaggat
     2461 atgccacaga tgttttagaa gaagaaccgg gacgtcaaga tcatccgtat ttagcgtttg
     2521 aacaagtagt gattacgcca catatatcgg cctacacaag ggaatgttta gaagcaatgg
     2581 gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca ccttcttcct gaacgttctg
     2641 tccagacaaa gagcaactat ataaaaggat gatgaagaaa atggtgaacg gtttaaaagt
     2701 gaaaacaggc cctcaatttt atttatatga agaaggtggt atttcaaaag tttctgatct
     2761 cttgaaatca tacggcgcga aaagagtact ggtcacccac ggaacggtat catgggaaaa
     2821 ggcgctacct aaattagtat ttttgaatga cgaaacgatt caattttttt atcatcgtta
     2881 ttctggagaa tgtagttatg ctgaagccag acgaatagct acaatcatca aaaaaatgaa
     2941 atcgatttcg tagttggcgt tggcggagga aaactgaccg atctagtggg ttatagtgcc
     3001 aatcttgcaa gaatcgattt cggagtcatc cctactttag ctagtaattg tgctccatgg
     3061 acacctcttg ctgtcatgta taaggagaat ggacaagcag aagggaaaac agaacacttt
     3121 tacagacaag ctgctttttt tataacagat ccccaattag tgattgattc ccctgttcca
     3181 tatttcattg ccgggttagc agatacacta gcaaaatggt atgaatctga gctcatctta
     3241 gaacaagatt ttttgcaaga tgaatcattt ttgaagctag cacaagatac tgcgaagatc
     3301 tgcaaagaag agattttgaa tcattctgct aaagcaatcc aagatatggc acaaagaaaa
     3361 ctgacatcgg agttcaaaca gctttcagag attatctttg ctgttgctgg tttagttgga
     3421 ggattaggag ataaatacgc aagaaatgtg gcagcacatg ctatgcatga cgctatcagt
     3481 aaatatattc cggaaagcca tcgttttcta catggagaaa aagtagcgta tgggattttc
     3541 tatcaactgg cactagaagg tcgctgggaa aaaatagacg agttatatcc tttgtatcgg
     3601 gaattagaac tgccggtttc tctagaacaa atgggattat ttccgaaaga taaacagata
     3661 atagataaaa tggctgaatt tattgattcg aaagagaaag tgcatttgat tcctatagag
     3721 gtaacacaga aatcactgat acagtcaatg atggaactgg aaggttatat cagaaagatc
     3781 tgaaggagga ggacacatgg aaacattaag tgtagtacaa agtttgttga ttgctttatg
     3841 ggtagccgcg gttatgtccc gctggttagg tggaggagcc acgttgacct tacgtttttc
     3901 tcctttgatg actggattag tcgtcgggtt gatcatgggg gaagttgcac aagcgatgat
     3961 tgttacagcc gctttgcaaa tgatttatat gggggttttt tctccaggag gatcaatgcc
     4021 agctgaacca tctattgcag cagctatcgc agtaccagtc gcattattag gggatctgtc
     4081 gccagaagca gcgattgccg ttgcggtgcc agtcggattg ctcggaagtt acttgtatca
     4141 gtttcgtttt ttcatcaata cttttttagg taaatataca gacaaagctg tagaagattt
     4201 gaatacaaaa gctatggccc gatcaattat tctttatcca acggttgcct catttttatt
     4261 attcgttccg cttgttttca ttgctttata tttaggggcg ccattgatcg ctgatgtaat
     4321 caaagcttta gagggaacag tcgttatcca cattcttgaa gtagtaggtg gagggttagc
     4381 ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga aaagattatc tggtcttttt
     4441 cttccttgct tattttatga gtgttgtttt gaaatcttta gatattacga tggttactta
     4501 tgccattttt gggattatta tcgctttgat ctttgtccag tcgcaaaaag ggaaacaagt
     4561 acagcctgcc acaagcaata acaattcagc atcttttgat gacgaagatg actatgatga
     4621 cggattttaa tttaggagga atgggaaatg gctgaaataa tggataatca aacaggtgct
     4681 cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc tgagatggca ttttgcaaac
     4741 gaaattcctc actcttttga acgttacttg gcgccatcac tactatacgg aatgatgcca
     4801 atcttgaaaa aactttataa agatgaagac tctttaaaag cagcatataa acggcagtta
     4861 ctgttcttca acactcaatt aagctggggt ggcggtgtga ttactggcct aatgtcttct
     4921 atggaacagc aaagagcaga agaagagtat gaagaaaaag agatcttgat gcaggatgac
     4981 ttgatgtata acacgaaagc gggattgatg ggggcactag caggaatcgg agatgcaatc
     5041 gattcaggaa ccgtccaata tatttttatc gcaatcgcgg taccttgggc acaacaggga
     5101 agtcctttag gcgcactttt tccttttatc tgctttgggt tgtatcaggt tttactaggg
     5161 gtatttttcg caagacaagc tttcactatg ggccgtaatg ctacaggcct aaagcaaaac
     5221 actggtgttc agacagcgat tgaaatgcta tcagttttag ggttgtttat gatgggggtg
     5281 cttgcgggaa actatgtaaa agtagaatct tcattgaaat ttgcgatatc cggacgtgag
     5341 tttgttatcc aggaaattct tgatcaaatc gtgccaggga tattaccttt agctgtcgta
     5401 atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag tcacacaagc attattatgg
     5461 ctgactggaa tattaattgt gctagcagga atcggtattt tataaaaaga agggaacgtg
     5521 aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc gtttgatcca tgggcaagta
     5581 atggtaacgt tatctcaaaa aagtggagtg aattccattt ttgttgtaga cgaagttgta
     5641 gcaaaagata aatttatgag agatctttac aaaaatgcag gaagccgtac aggacaaaaa
     5701 acaattgtca ttacaccaga gaaagctaaa ttctattggg atgaatatca atttaaagaa
     5761 tacaattgta ttttgatcgc aaaaacagtc caaacgattt acgacttagt aaaacacgga
     5821 atccccatgg aagaattgaa tattggaggg attgctcaaa aaaatcctga tacagatctt
     5881 ttagtgacga aatctgttta tttaaataaa tcagatgctg aaaaattgaa agaaatgaat
     5941 gaacaatatg gcgtaaagaa tatttatttt caagccactc catctgcacc taaaacagga
     6001 ttaaaagatg tattgaacaa gtttgatttg tcataaacaa atgcttagaa aaaagataaa
     6061 gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt gggaatttcg ttatcgctat
     6121 atttaccgta agcgccgtac aaaaaagagt aaacagcgtt ttctttcagc attagtatca
     6181 gatatttaca gtatgagaac agatgtgaca gtcattgctt acgatacgct agcctaccga
     6241 tcaaaaaata tttatgttgg agatatcgaa aaagcagaga aagttatctg tacttattac
     6301 gatacacccg ttcatgcatt aggaagttat ttcatgtttg attggaaaga ccaaaggaaa
     6361 aaaacaatct actctatatt attgtccttc attttgttgt tcagtttagg ttggtgggga
     6421 atgatgatat ataacaaaaa tcctcatcac gtattcgatc tgctatccgt ataaacaggt
     6481 atcactgtac tagcatttgg gagctacttt ttcttattgg gaaaagcagc tcgcggatgg
     6541 cctagcagac aaacttttat tcgaaatact tcatccatat taacgatgct agaaatgatc
     6601 cgtacgattg atgatccgag tgtagcgtat gcgtttgttg atgaaggctg ttatggagaa
     6661 aagggcttag acagcgtgag atcgggtatg aaaaaagagg ctattctttt ttatctagac
     6721 tctgttgggg cagatacacc gctacagttt tcgggaaatt atttttcaaa taaagaacag
     6781 tggctgaaac aagttgataa gttgaaagaa aaaaacgtca attatatttt tagtgcaaga
     6841 aaaaagcaag cgcagttctt ctatttgacc aaaacagatt tacgagggaa aacatttaat
     6901 tggcaaaatg ccaatcagat tattgctctt tttcgataag taaaaagtgc aaaacaagga
     6961 gtgagaagac taatgttgga aatcatgatc gttacacatg gtaagttgag cgaaggactg
     7021 aaagatgcgg cggaagtaat tgtcgggaca tcgagccata ttgaaacaat gtgcttagag
     7081 tctggggagg acatccaaac attcggagag aaggtcaaag aaaaattaac tatgatcaac
     7141 acagcagaac ccatgctagt atttgttgat ctaattagcg caagtccgta caatcaagcg
     7201 ctcttggcag tcaatgaact gactgaagaa aagaaagcag ccacttatgt catagggggc
     7261 gtgaatctgc caatgttgtt agaagcaatc aatcatcaga tgcttcacac accgatagaa
     7321 gaagcggtgg aacaaattgt caaacaagga aaagataatt taactgtttg gcatgcttca
     7381 gcggaagaaa aaaaattaga tgaggacgat ttttagaagg aaggctgaaa agatgaagtg
     7441 gggatttaga tggtatggtg aaaaagggga ttcgatccca ttaaaatatg ttcgccaaat
     7501 accgggaata aatgggataa ttggaacttt gctgactaaa atgcctggag atgtttggga
     7561 gatttcagaa attgaggagt tgaaagcatc tgttgagagc caaggactgg aactgcttgg
     7621 aattgaaagt gtagcgatcc acgatgcaat caaggcaggg acagatgaac gagattacta
     7681 tattgataac tatatacgaa caatcaaaaa cttgtctcga tgtggggttc gattgatttg
     7741 ttatagcttc aaaccaattt ttggatgggc gaaaactgat ttgtttaatg aaaacaacga
     7801 tggaagctat tctcttttat acgatcagaa aatcgtcgat cagatggagc caggagaaat
     7861 gtatcaattg atacacagcc agtcaaaggg attcaaattg ccgggttggg aagaagaacg
     7921 gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga atgaccgaag aagaattatt
     7981 tgaaaatctg acttattttt tagaaaaagt gattccagtg tgcgaaaaag cagatgtaaa
     8041 aatggctatc catccagatg atccgccatg ggaacttttt ggttttccta gagtgacgaa
     8101 aaatcttggc gacttgaaga aaattctaaa tgcagtagat tctccatata atggactaac
     8161 gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat ttagttgaaa tcatccatga
     8221 ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg gagtttttag gagaaagaaa
     8281 gttcaaagaa acggcacatc cctctacaga aggctccctt gatatgtatc atattatgaa
     8341 agcgttggtg caagaagggt tcgacggtcc tatacgccct gatcatggta gaaccgtgtg
     8401 gggtgaagtg gctatgcctg gatatggcct gtatgatcgt gcaatgggac tcacttacat
     8461 gcaaggtcta tatgaggcga taagtaagga aaaaccactg ggagagcatt atgaataaaa
     8521 tcaagctctt catctttgat atggatggcc ttttattaga aactggacgt ttagcttatc
     8581 gtgcttatgt aaaatcagca caaaagtatg attatgaaat gcgtaaggaa gtctattatt
     8641 tactaactgg ccaaacagaa atggcgataa gagaacaaat gggcattttg tacggagaag
     8701 atgttccata tatcaaatgg cgagaagcaa tcaatcaata taaagaaaaa atcgtgaaag
     8761 aagataagag agtatatacg aaaaaaggag cagaagaaat tttatctttt gcaaaagaaa
     8821 gaggaatcca tacaatcgtg gcttcgtcta atacacgggt aaaagtagaa atgtatttaa
     8881 aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc aggggatgat gtaaaaaaag
     8941 gaaagccgga accagagatc tttttgaaag cttgttcaaa aatgaatatt cctccaagtg
     9001 aagcacttgt atttgaagac tcgattgcag ggatcgaagc agcaaggcga gcgggcattt
     9061 tgtcttttct catcaaagat catttgatag ggttgcccga tcataatggg aagcataagt
     9121 tgaaagtaga tgtaagttta tatgaatcca ataaagcaga ctatgaattt tatgacctgc
     9181 atcaagcaaa acgattttta gaaagtaaag ggttgtatct ctaataaaga ggactatttt
     9241 ctagtcctct ttatttattg tcctgcacga ctgagggaac agatgaaaaa gaagtaattt
     9301 tttattttga agtatctacc ttatttgtag tgaacaattt agaacaaaca caaaacgcat
     9361 ctactgttat ctaccagtag atgcgtttaa agagaagaac gagaatcgtc catatgaggg
     9421 tttccacggc tctataattt ctaggactga tagaaaatca ttctatcggt cccttttgag
     9481 catgaaaaaa caaaacatac agaagctccg ttagaattga atcaccacaa cacaatcaaa
     9541 ggagcgttct gtatgcctga tcttcatcat atcaaactac tacttggaat caaagataaa
     9601 aacattttca tcacaaatgt ggaatcgaag agcattaaga atatcaagag tcttgttgtt
     9661 tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt gtaaacagat gaatcatgaa
     9721 ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc aacttaataa atgtgcgaac
     9781 caactgactt atcttgcttt agcgaaacaa cgctaccact gtcgtggctg ccacacctat
     9841 tttacggcga atacctacat tgtcgataga aactgcttta tcgctaaaca agtgcgctat
     9901 aaaattttag aagagctaac tgaaaaacaa gcaatgacga caattgctaa gcattgcggc
     9961 gtttcttggt cgaccgtttc tcggacactc gcttctctga tccccatgac aaaagtaaaa
    10021 agaaattggt taccgcgttg tttattaatg gatgaatttc gttcactgaa aaatcaagtc
    10081 ggtccctact ccttttcttg tatggatggc gatactggaa aattactgga tattcttcct
    10141 tcacggaaga aaaaggctct cgtttcttat tttatgcaat ttgaacgacg cgcacgattg
    10201 aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg cctctctgat aaaggaatgt
    10261 tttccaaagg caaaactcgt tgtggatcgt tttcacattg tcaaacatct cattcgtaaa
    10321 tttgaggata ttcgcgtacg aatcatgaaa agctttgatc gaaacgatcc aacacaagcc
    10381 aaatattatc gacaattaaa ggcgttgtct cggcttctga tcaaacgaca ggacaagctt
    10441 gtttatgaca aatggaccaa atggcgcaat tttggttggg cgtatctcac cgaaagtgaa
    10501 gtggtggatc gtctgctttc gatctctgac gaattacgaa tcgcctattt ttactatcaa
    10561 gaaattcttc aagcgtttca cgacaaagag gcggacactt tctttaaact tgtaagaacg
    10621 atgcccaatt ccgttccaaa agaactgcat tctataaaaa aagcttttat aaactatgaa
    10681 tccggcattc gtttagccct cgaattgcct tattcaaatg ccaaaataga aaatctgcat
    10741 acccacatca aagcattgaa acgagtcgct tatggctttc gaagctttcg gaaaatgaag
    10801 accagaattt tcttactaaa taatctgatt acctatgaat caaaaaacat ctaaagtaga
    10861 aaaggaattc taccttagat gttcttatct cactctatca gtcctatttg acaaagagcc
    10921 gtttccactc cttatgcaga tttttttaat tcttgcactg cttgttcgat cgtaacacct
    10981 gtcgctacat gattggtatt ttctttgtca aaaacgacaa atagattcaa atctgtatcc
    11041 aaggttactc gatattgtat atctttgtta tagaatgtca tagccattct cctttcttta
    11101 aatacttgct gaaaggcaac gaacgcgttg ataacgaaat tccatttata agtataccac
    11161 caattgaaag gttttcatag ttaagaaatt ttaaagtcaa aagttgcaga taaaagcaaa
    11221 taaacaagaa aacaaaaagt gaatcaatcg cggttattca actttttctt cgattcgaag
    11281 acaatagaag acaatgaatt ttatatttca ttgcattatc cttgtgttat tagaaaaagc
    11341 atgatatatt aaatgagtaa tctattttga aacgtaattt gagcgatata tctacataga
    11401 taaaactcct tttgcctatc aaatatttat tgcaacgaaa aaacgtacat tgatacgtat
    11461 caggaggaat ttatttatga ataacggtac agtaaaatgg ttcaacgcag acaaaggttt
    11521 tggctttatc actggtgaag atggaaatga tgtatttgca catttttcag ctatccaagg
    11581 tgacggcttc aaaactttag aagaaggcca agcagtgtct tacgatgttg aagatggtca
    11641 acgtggccct caagcaacaa acattgttaa agcttaataa aaattgactt ttaaccgtct
    11701 tttcgaaaga aaagacggtt tttttgtacg aaaaaattca ttggtcgttg aatgggctga
    11761 atagtagtgt ataaaacaat tttgtggagc gtgaagcttt tatggtagag gaaaatacga
    11821 ataaagtgat gttgataaaa agattcctat cttgaatatg aatcgagatg tcttgaaaat
    11881 gaatgagttt gaccaatcat tatcctatcg aaatttacag tttcgttcga ttggtctgtc
    11941 aattaatgaa aaagtaaaaa agaatatgtt ctaggaagag ggcggcacat ctaaattttt
    12001 aagtaacgga cagttcaaat atcaagcatt gaaagaggct atcctactgt ttgaaggaat
    12061 acgaatatat aaaaacgaca aagaggaaaa agtagttcat taaatccctt caagtgatga
    12121 aagaatgaga tctaataaaa cctgatcttt catctgtttt tttaataacg taataatttt
    12181 tttatttttt gtgctttcgc cgttattgta gagtgattta gcattcttat ttttaaaaat
    12241 aactgtgtta tatattaaaa aaggcataat agttataagc ttttttaaag aatatttaga
    12301 aattattaat tttcgtctga aaacaaaaaa agaaagcgtt attattttag tctattttca
    12361 gggggggaac cttatgataa aaaaactatt tttactatca atattaacgc tagcaatggc
    12421 aggttgttct gactctggct tagcaactga actgatcaca aaatcaactg aagccacgac
    12481 agaggagtta aaatgtggaa acgatcacag tgactacaga ggcttaaatc gacgatgcat
    12541 gggaatatta ggcagaagca attgtaagca tcgatgtagt aatcgaagaa ggtgtagaag
    12601 aagcaacaga aaaatcaaaa gaaagatcaa aagaagcaat ctgtgctgtg catcgtatac
    12661 agacgtcgaa gatgcggtac ttttaaacca cgtcaggaga tgttcattta tgtctaggga
    12721 agttggcgat gaaccaaaac tagagtacgc ttttagtttt gcacgtgagg atgaagataa
    12781 ccctaaaact tacgacattt tagcttgatc taagatggtt aaagcttttt aatgaagtaa
    12841 gaggagatag aaggcttttc tagttatatt aggtaagata aattcctttt tgctccaaat
    12901 ttgctaaaca ttctgctaga taaacttcat cgtgttccgg ataaatcggc acgttgcgct
    12961 ctacagtagg taaatcaaaa aatgcttcgc atgatttgcc acgatacata ggatttttcc
    13021 actctggtgc tgggctgtaa ccacgttttt ccatttcatc catgacaagc tgatgatatt
    13081 ggaataatct ataaggagaa tagtgaaaaa cataatccac agtagcatgt ttctttcccc
    13141 agccatttcc tcttaatgca cagcattccc gatgttgtcc taaaagctgt tgacgaggaa
    13201 gctttgaaat caatttttca tgccataatc acatagaaat gtctccttta tatatagatt
    13261 gttaacctta gacgttaaat gaaaaaacca gcaccaaggc tggaaaaaat cacttcagtt
    13321 tgcttgctgt ttctgagcga ccaattaggt aatcaacgga aatatcgtaa aagtcagcga
    13381 tatagattaa ttcaatgatg cttggaattt cttctccttc ttcgtactta cgatatttga
    13441 ataaaggaat acttaacgcg ctagaaacag ctacttgaga taaagatcgg ccagttctta
    13501 agaaattcag acgacaacgt aaagtatcca gtaaaatagc ttcttcgata ctcatttaat
    13561 cacctttcat tatagtagaa tagttataat gactatagaa agctacacat gcaatcaata
    13621 gtactcaatg ttttttattt aaacgtatca aataaagaat gacgatcaat aaagacaaaa
    13681 ccagtcctga taattcttct ataaaaacac tatcgggttc aaagtagttg taattaaaaa
    13741 ctttccacat aaagtagata gccggaagag tcaaaatagc taaataaaac cgttctttgt
    13801 tccaattcat aagataaaca atacttgcga ccagtgcgac cgcaggcacc caaaacaaaa
    13861 ctctcaccaa cgtaatcatg taacggactc ctcaacgatt catataataa aagataacat
    13921 gttattgcat acatcacaaa tgaaatgcta ggaaaataaa agaagaatag atcaatgaaa
    13981 aatgaaaaag cagaacagtt ttctgctgat aagcaggaac tgttccgctg aatgtgtctt
    14041 atctatcgag aaaaaatcaa tggattgata tctaagtaat tcaatacata taaaaagagg
    14101 acgatcgcat tcactaaaaa ggaaagaatg atgatcccat agcgaatgga aacatattta
    14161 acaaatactt ttgtttgaat acgggcatat ctttttcctt tatagctgag catcatgaca
    14221 agaagcaaaa gggtaatcac accttgaaaa atcattccaa taaacagaat tcctgaggtt
    14281 tgataattta attggagaaa ataagctgct gccgcaacta aatcgaataa aatcgcaata
    14341 aatggcaaaa taatcactcc taacattgag aataaataaa agaataacat tttttctaaa
    14401 ataagcaaat aaaaattaaa gtaacaaaat agttatgaca caagtaaaag aaataagaat
    14461 aacaatatta ttttacttat attttgaaac ttgtgagagt ttgtcattcg tgctatagtt
    14521 gaaaagtaac aaaaataaat tttatttatg atgtaccttt agctcagttg gttagagcag
    14581 acggctcata accgtccggt cgtaggttcg agtcctacaa ggtaca
//
LOCUS       DACELF010000067        14192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.070, whole genome shotgun sequence.
ACCESSION   DACELF010000067 DACELF010000000
VERSION     DACELF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.070"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1127)
                     /locus_tag="IW687_001996"
     CDS             complement(<1..1127)
                     /locus_tag="IW687_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0951253.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1210..1545)
                     /locus_tag="IW687_001997"
     CDS             complement(1210..1545)
                     /locus_tag="IW687_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0951254.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1555..1929)
                     /locus_tag="IW687_001998"
     CDS             complement(1555..1929)
                     /locus_tag="IW687_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0951255.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1942..2256)
                     /locus_tag="IW687_001999"
     CDS             complement(1942..2256)
                     /locus_tag="IW687_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0951256.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="IW687_002000"
     CDS             complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="IW687_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0951257.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            6384..6668
                     /locus_tag="IW687_002001"
     CDS             6384..6668
                     /locus_tag="IW687_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0951258.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            6671..7588
                     /locus_tag="IW687_002002"
     CDS             6671..7588
                     /locus_tag="IW687_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0951259.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            7581..8363
                     /locus_tag="IW687_002003"
     CDS             7581..8363
                     /locus_tag="IW687_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0951260.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            8364..9044
                     /locus_tag="IW687_002004"
     CDS             8364..9044
                     /locus_tag="IW687_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0951261.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            9037..9933
                     /locus_tag="IW687_002005"
     CDS             9037..9933
                     /locus_tag="IW687_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0951262.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            10128..11777
                     /locus_tag="IW687_002006"
     CDS             10128..11777
                     /locus_tag="IW687_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0951263.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            12371..13306
                     /locus_tag="IW687_002007"
     CDS             12371..13306
                     /locus_tag="IW687_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951264.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            13299..14069
                     /locus_tag="IW687_002008"
     CDS             13299..14069
                     /locus_tag="IW687_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951265.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         4183 a         2724 c         2829 g         4456 t
ORIGIN      
        1 tgatcgcgga ttccgtctcc taaatatttc gcatccggcc tttggcaagc caaaatcaga
       61 aagaaaccag attgacgacc caacatcacg atttgtttga gcttattcaa aatcaccgca
      121 ctttctttcg tcgttaacat ctccatataa gccacgtatt catcaaagat taaaaagttg
      181 ggtggaagtc caagataggc ataattctct cctgttttgt agttagacat ttctttcatt
      241 gccttgctac gagccatcat acgttcatag aaatcttcca cacaagcaga aatttcttcc
      301 ttttgtgaat aaacatgagg catcaccgta cctaaatcag ctaaatctgc atttttggga
      361 tcgaggataa acaattctgc atctgacttc aatagtgctt cgataatggt gaggaggaaa
      421 taggtcttcc ctccacccgt gccaccagcg attaacatat ggggtaaaga atcataagcc
      481 catacttgat ttttcatcaa tcgcagagtc ccattttctg ccactacttc gtctatccca
      541 attcgattgg cgatcatatc atacagtaaa gtatactcca cataggagtc tttgagttcc
      601 ttttctacta actcacaata cagtccgctt tctaacttct tttccaattt taaaagctga
      661 tcttggtatt tcccaagtga aatttgaacg cgaatagaca gtaatccatc tttcattcga
      721 taatagattt tggggaagta actgatagtc tctctggttc ggctactatt caaatctttg
      781 aaaaagcctt cactttgagt ttgtttcact tcataccaat ggttctctag aatcatccga
      841 gctagctttt gtcgatgcca caagcttcgc caatgatcaa gaaaaaagtg aatataaagc
      901 aatatcatag ctcctgaaaa agcgagactt ccaagcagac atactaaatc ttttatagac
      961 caaacgattt caatagtaga aagtgatagc ttagaaatat caaattgtct aaactcttga
     1021 aaataaaaga cgctcatcca accgatgaac agagcgagta gccagccaag aacaaagtga
     1081 taaaccaagt gctgatctcc tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc
     1141 acctccaatc gaaaaagccc aataggattt cattaggcaa tttcaaaaag ttatgctagt
     1201 catttactgc tacgaggagg ggagttgttt cgtttcaacc aattgattta aaaagccttc
     1261 aatagcatcc ggcatctcca caccaatttc ttccaaaatg gattctgcct gccgacataa
     1321 gttttcattt acttcgacaa ctataaagaa agactcttct tctcctaacc agtctagtac
     1381 atcagagtct tcttcactgt cgctattttc tccaagtaaa ccttgatgta taatcagaaa
     1441 cattttttca tcagaggtta atgctttaac gagttgttcc tcagtcagct cgccttttcc
     1501 atattgatcg agacaatcta aaatatgttc aacattaatt tccataatct tcacctattt
     1561 ctttctttca ttttggttcg taggatttcc ttgattcggc tgccgcttca agactaaatc
     1621 atccgcctta atgaaccaat taacggtagt tccttctcga aaaacataat tggcaacggt
     1681 atccacgatt ggattgacca actctacctc tgcatcataa gcaaactcgc gtaagggtac
     1741 attagctggg atactaactt gaatcattcg cccttgtgca gaggatttta aattataggt
     1801 tcgttctttt acctcattac caattgttcc atcctcattt tgcttccgta cttctcggcg
     1861 taatgcggaa aaacgcaact tcccaaatgt taaacctgaa tctacgacaa taccttctgt
     1921 taatctcatc ttcttttcct cctatgcttt cactaaatca tcaacataca acacgtaatc
     1981 agtaaaccca cgattctcaa tctcatagcc tttggcaacc aaatggggat tcacggcttt
     2041 tagcggttgg tcttgatcaa aatccttttc accggcttcc gcagctacta ccacctcaat
     2101 atcatcggcc cgttgcttac tggaatacaa atgataactg cgaccaatca ccgtagtatt
     2161 ccgtccatat ccttctgtta taatttctcc ttcaccggca taacttaact ttccaaaggt
     2221 ttcagccata tctggcacga caaatttcaa ttccatcttc ttttcctctt ttcattgtta
     2281 gttttggata caaaaatagc gcgactactt ttttaggagg cggtcgcgca cgttggttct
     2341 taggatttac ttgtatcatc tcatcacctt agttccggcg attccagaaa taatagcccg
     2401 ccgtcgcaaa aatcaaagta aaaccaatga acagtaaaac attggaattt ttctccccgg
     2461 tttgagggaa ggtcttcact gtgggactag attcttgaga gccacttgga acagttggtg
     2521 tttccggttg tttcggcgta cttagtatgg ttggaacttc ttttggtgtt aaggtttgag
     2581 atttttcttt caaatcttca ttgtgttttc cattgatatt cccatcttta tcgtaattga
     2641 tttccgcaaa agtaaacttg gttccttctg gatattttcc ggtatctact tttttcgcaa
     2701 gcacagtttt ggtgaattct ttatcattta cttcataatc aatcttgcca gatttccaaa
     2761 tttctttgtt tgtaccatcc ggtaataaag catacagaat tgtttcgaaa gcttcttttg
     2821 agccatccag tacatcatga gtaatcgaca catcatcaaa catatccacc acatcaccat
     2881 gagtaaaggt ctgagaaccg ttttctaggt gggcctttgt ttggatagaa acatggtgtt
     2941 ccactgtaca actgacagtt tgggcttggt tattcaagct aacatccttc gctactggct
     3001 tatcgcctgc ttcaaaagct tccttatttt catacacata gttgaataag acaatagttt
     3061 tatttaaaac ttgttcggcg gttaatttat gctgaatttt ccaagttcct gttttctctt
     3121 tgttcgccaa cgttgcgctt gtttctgcaa tcaccactgg tttggctttt tcatcttttt
     3181 cttgggcagc ttttgtcgca tccacatcaa tggcttgcgc taccacatac cagtcttctt
     3241 tgatttcatg tagattgtaa ctcaatttat caactagctc ggtagaatcc agagaagtta
     3301 attctttgtt tccgtctttc caagtcgcaa gagaagttaa actatcttct tcctcaggca
     3361 aatcatagag cattaaacgg ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt
     3421 aaggaacggt caccgtttta attggttgtt tttggtcttg ttcggtaatg gtaaagacat
     3481 actcactctt cacaaagtct tctttgtttt ccttaaaagt agaacggatt tctaatggcg
     3541 taatcttttg aaaaccttct ggggcttcta cttcttcaag taaataatcc ccataaggaa
     3601 gattttcaaa cttgccataa ccatcaaacc ctaattgctc gttataagct gtggtcgctt
     3661 catcttcggc acctgtgatt tcattggtcc cttccaatgg cgacacttta aaggtcagat
     3721 cattgaagcc agtttcggta gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc
     3781 caaagcgaat cacttgttct tttgccgtaa catttcgggt aatcacggca tttttttcgc
     3841 tatcatccac ctttttgatg gatacaggat actttgtttc atcaaggcta tatccttcgg
     3901 gcgcttttgt ttcttgccaa taatactcgt tgatagctaa atgtttaacg gcaacttggt
     3961 tcttttcgtc taaagtcaaa gtgaccgttt catcagaacc tttcgttccc ttcgctaact
     4021 ctggtttaaa agcctcactc catttaacag cttttccatc cttagcagta aacaaggtgt
     4081 attcagcacc ctcaaagact gccttacctt gtgctttatc cccagtatct ttgtcttctt
     4141 tcgtcaaagt ggtttcgcca gtgacttctt gattttgccc ttttacgtta ctggtaacaa
     4201 gggctacggt ttgattggca tacttcaatt cgacttttac aggtttgaac gtattcacga
     4261 acccatgact agctttagtt tcggtcacat aataggttcc caattccaac gcattagcaa
     4321 tctcttttgg tgtttccgca tgaccgtttt catccgtggt catttcttga acgatttcac
     4381 cggttggact gtctttgcga atggcaaacg tattaccagc taaggaataa ttgtcattcc
     4441 aaagatcact ccctgtttca actccgcttt tatccagaat gatttgccct ttttcccgtg
     4501 cgttttttga agtcacgtaa atggtttcgc ctgctttaat ggtggtagtc atgggggtgg
     4561 tatcaatcgt ataaggcgcc ggtactgatt tttcggtaat ggttactttt gtaccatgag
     4621 gaatctcatc caatgtagca atgccttctt tgtcagtagt cacgtctttt gcaggtaaag
     4681 tttttccaaa gtctaaatgg aaaaccgttc ctggtacaac atcacccgat tctttatcga
     4741 cttttttgat ctttaaagaa ccctccgttt ccacatcaat tttgattgtg taggtattag
     4801 gtttatcaat cgccccggcc attaaagtct gttggccagc ctttttatag gcgacaggtg
     4861 ttccggtacc agcagatttc ttaagcgtca gcacaccact ttcttttgaa ttggcatttg
     4921 gggtgattat caactgatta ccattcacac gataatcaat gtttgcggca ttttccacaa
     4981 cctcatcgaa ctctgacaaa ttcagattat tcgtatccgt cacagtagtc gattgcccta
     5041 atactgtttt agccgtacta ttatggaagg ttggtttctt ctgataatcg tcaatggctt
     5101 gatttatttt tgcctcaatg gctgcgatat ttacggcact accatctgat cgttttattg
     5161 agtgaagggt atacccatta acttcttgcc agatcatctt ctgtgccaca atatgagtat
     5221 ctgcatctgt acctactttt ttccataaaa cggaaactaa tttggcttta tcggacatat
     5281 ctggtaacgg gtttttctca tagccaggag tgaactcatt gtaaatcggg actcctggtt
     5341 caatacagaa aatatcttgt ttaaagccgt tgtacactgc ataatgcgga acagtgactt
     5401 ctgacattgg acgaccatca ctaaaagttc cttcaacagc atattgatgt gctaaatcta
     5461 gttctacggt taccgtttgt ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa
     5521 ttgctccaac aattgcttga acagccaaac tcaaaatagt taatacacat aatttctttt
     5581 tttgtttttt atacatgaaa tgttctcctt tcaaattaag cacaaaaaaa taagatgctc
     5641 gcttgaacat ctttgtttat aaagtctttt taaaattttt attgactgaa tagaatggat
     5701 ttgagaatga atgagcctaa aaatttaatc taaaatacga aatatactgc ctttaaatta
     5761 gaacacattt tagaacacaa acatttcaaa tttaatcaaa tataataaaa tatatttacg
     5821 ggaaaacctt tataaatcaa ggtttttcaa acttattcaa tgatacgaag aaaaaaccag
     5881 gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga ttatcgttcg
     5941 agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt taccttaatc
     6001 ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt ttagctatgt
     6061 taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg ggaaaaagat
     6121 aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa gtggtcagct
     6181 tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat attgtgactt
     6241 gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg tgaaaagtct
     6301 tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg tattattttt
     6361 cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc tggcttcagc
     6421 gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg attaccatgg
     6481 tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag acaactcaac
     6541 cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta cactttcttt
     6601 atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc aataagaaag
     6661 aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta taaccaaaaa
     6721 atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg gaatgatcta
     6781 tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta ttatgggttt
     6841 gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc aggcagcaag
     6901 acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa ctgcaagaga
     6961 taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg aagacctgct
     7021 gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct ctttaggtat
     7081 gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt tgattttgga
     7141 tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa tcatccaacg
     7201 tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag atgaattgtc
     7261 acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag aattatcgaa
     7321 agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg cgacggaaca
     7381 agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat ctagccgtgt
     7441 tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat tggtagaggc
     7501 aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt atttcttaca
     7561 attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata aaagtatgga
     7621 agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt ttttattctt
     7681 ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca acggatacag
     7741 tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg attcttctgg
     7801 ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt acgattcgca
     7861 atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt ctcgttacgc
     7921 ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt gcttacacac
     7981 ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat gttctaaaag
     8041 tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg gctttgacat
     8101 tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc atgggaacgg
     8161 ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg gagtggttta
     8221 cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg caagcaccga
     8281 aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga atgtttattt
     8341 tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt atcgaagacg
     8401 atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa cttcttgatg
     8461 cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat ttgatccttc
     8521 tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc cgacagacat
     8581 cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa gtttccttgt
     8641 tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa ctgttgttac
     8701 gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg tcgcaacgca
     8761 tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg aatggtgtgg
     8821 ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa catccgaaaa
     8881 aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat tttgatagtg
     8941 agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag gcacatgctg
     9001 tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct gttgcttctc
     9061 gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa gagattaact
     9121 aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc agtcgaattt
     9181 cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga tgaaaatgaa
     9241 caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat cgtcggcctt
     9301 tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt actggaacag
     9361 gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc tgttgcaaga
     9421 ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa gcaaaaagaa
     9481 atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt ctctttttat
     9541 gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat aaatacaact
     9601 gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca gaatttattg
     9661 cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt cattcgatta
     9721 acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt cttccagcgt
     9781 ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg gctttacctg
     9841 tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga cgggggcatc
     9901 ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct gaagtgaatg
     9961 acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt tttattttgg
    10021 tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt ttcaaatagc
    10081 gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg ggccaaagag
    10141 aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta gacatttttt
    10201 cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct tatcaaccga
    10261 gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa ttccgttcat
    10321 gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa gtatttgaag
    10381 ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt ttacgtagct
    10441 ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag gaaaatcctc
    10501 aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat cataattttg
    10561 caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt ttatttattt
    10621 ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa gtactaggaa
    10681 tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg aagaaacaaa
    10741 cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca gccgccaatc
    10801 agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact aaaccattaa
    10861 gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa gcagaattga
    10921 tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat ttcgttctca
    10981 ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg ggagaattgg
    11041 aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc cgaccagagt
    11101 ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa gaagcggtag
    11161 ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca tcaggaaaat
    11221 ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc caaacagcga
    11281 ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca atcgaagatg
    11341 atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa cgtttgctgg
    11401 accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta aaaggaacaa
    11461 atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg gagtatccat
    11521 ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag aattattgtg
    11581 agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt gatttcaaat
    11641 taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt ttgaattatc
    11701 ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa cagcgtcagt
    11761 ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa aaagacgatt
    11821 atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac gagtctgctg
    11881 tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg ctgtcttcct
    11941 tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt ccatggcaaa
    12001 gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc attttattta
    12061 tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag cttaacatga
    12121 ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta gtagttgctg
    12181 tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc tatcgaagat
    12241 gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg tgtggaagcc
    12301 aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc tataaggaga
    12361 taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct atggaagtgg
    12421 ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct atgcgctatt
    12481 agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt tagtcaataa
    12541 aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag ttcgagcaaa
    12601 gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa cggtccagca
    12661 aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga tgaaacgcag
    12721 cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg cacgtatgct
    12781 ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg aaccacgttt
    12841 gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg agatgtggga
    12901 gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc attatctaga
    12961 agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac tgatcgagaa
    13021 tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct ttgatctagc
    13081 gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag atgagcagac
    13141 aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc agcttacaag
    13201 acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag aactgttctt
    13261 gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt tattttactg
    13321 cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg gtccaaacac
    13381 tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa atgatcggtg
    13441 ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct ggtttgatta
    13501 tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc tctcaaaaat
    13561 ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg attatttggg
    13621 gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta acgatcattt
    13681 ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt acattattga
    13741 tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct cagtcttatg
    13801 atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta ggtggcgttt
    13861 tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc aatccgatcg
    13921 tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg ccagtagcaa
    13981 catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact tggtatttga
    14041 tcaataaagg aagaggattg agaagttaga aaagaggttg tgaaatcagc gttttgacct
    14101 gagtattaga gaaacaaacg ggaaaatagt tcctcatatt ttgttgtttt ttgagcgaat
    14161 accgagggtc agctgattga acaccgttta gt
//
LOCUS       DACELF010000068        14146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.071, whole genome shotgun sequence.
ACCESSION   DACELF010000068 DACELF010000000
VERSION     DACELF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.071"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(571..1725)
                     /locus_tag="IW687_002009"
     CDS             complement(571..1725)
                     /locus_tag="IW687_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HAQ0951266.1"
                     /translation="MKNLSYARRTTNESLCLELIMNNQNLSRADLSNLTGLTKATISV
                     IVKKLIEDHLVLETGIGSSGNAGGRKPIILKYNGKAGLSISIDVGYNYINALLSYLDG
                     EEIRIIQKKPIMLDEKEVKAYCSEIFASFEENLPKTSYGVIGVVVAVHGPVYNGELIF
                     TPHLNLEHIAFREIIEEISDYPVYLENEANLSALGEYTFTSNSSSLISVNMHSGIGAG
                     IVINGELQVGSHGFAGEIGHQIIVIDGEKCPCGNHGCLEMYAANKVCFEKFSKIKSMD
                     YVNAVILREYYQNNDLDAQQIIQENVHYLSVGINNLIAHYDPEVIVLNSSLYREIPEM
                     IHHLKQYLSNRFSDKIIVRNTVLNEKATLLGGLVLNLQHFLNINQLKLPH"
     gene            1986..3293
                     /gene="xylA"
                     /locus_tag="IW687_002010"
     CDS             1986..3293
                     /gene="xylA"
                     /locus_tag="IW687_002010"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAQ0951267.1"
                     /translation="MSYFPRVEKIKYEGVKTKNPFAFRHYNPEEVVAGKTMKEHLRFA
                     VAYWHTMTQDGSDPFGLPTNQRTWFGATEMDTARNRVEAFFEILEKLGAEYFCFHDVD
                     IAPEGNSLAEFYKNLDEITDLIKEKMDQTGIKLLWNTANMFSHPRYVNGAASTNNADV
                     FAYAAAQVKKGLDISKKLGGENYVFWGGREGYETLLNTDMAFEQDNIARLFKMAISYG
                     EKINHKPQFLIEPKPKEPSKHQYDFDAATALQFIQKHGLEGEFKLNLEANHATLAGHT
                     FEHEIEVARTNDALGSLDANQGDVLLGWDTDEFPTNVYDVTLAMYGVLENGGIAPGGI
                     NFDSKVRRSSFEMEDLFLAHIAGMDTFARGLKAAAKLKEERFFDDLKEKRYASFKEGI
                     GASIVSDKEDLESLTDYAMKLKDIQLESSHIEYVKSCLNDYLV"
     gene            3393..5003
                     /locus_tag="IW687_002011"
     CDS             3393..5003
                     /locus_tag="IW687_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0951268.1"
                     /translation="MKIQNPVLKGFNADPSIVRAGDTYYIANSTFEWFPGVQIHESKD
                     LVHWQLVTRPLSTIRLLDMKGNADSGGIWAPDLSYADGKFWLVYTDVKVVDGAFKDCT
                     NYLTTAEAIEGPWSDPIHLNGVGFDASLFHDDDGKKYLVQMEWDHREYHHAFNGIKCT
                     EYSVEEQRLLPETAKIIWRGTDVKLVEGPHLYKLFGYYYLFCAEGGTSYAHQEVVSRS
                     KTLYGTYESQPETFLTAFDAPDNALQKCGHGSLVETPNGEWYFAHLTGRPWHHSYESA
                     HDPRGWCTLGRETAIQKVTWTNEGWPEIVGGKQGSMVVDAPKGAEPFEYVSNTVVDDQ
                     FDQEELNIHFQTLRVPFSDRIGKIENGQLHLNGLGSLANVHENSLVARRWQAFEFAAE
                     TKIRYKPTTYQQMAGLTNYYNSTHWSMIQLTWNEVNGRVIEVTENNQGVFKSYLKEAA
                     ISVPEEIKYVYFKTIVSTSTYSYAYSFDGESWIDIPVQLDAKVLSDDYVNSSYGGFFT
                     GAFVGMVNVDYSGYNLPAVFDYFRYQEV"
     gene            5053..6561
                     /locus_tag="IW687_002012"
     CDS             5053..6561
                     /locus_tag="IW687_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951269.1"
                     /translation="MENELLFSMTDIEKSFGPVQVLKKANLEVRKGEIHAFMGENGAG
                     KSTLMNILAGLIDKDAGKIIFEGKEIPKMTTDLATELGIGFVHQELNLAEDLSVAENV
                     YIGRLPYKNKPLGIVDFKKLHEDTMYWLNLVGASVSPDAHVGSLSTANKQLIEIAKAL
                     SLNAKVIIFDEPTTSLSDKDVEVLFIIIRRLKEKGFSSIYISHRMKEIFELGERITIM
                     RDGKYITTDDVKDLSEDQIISMLVGREINDLYPKQATEIGETYLEVDQLSDRFGHVKD
                     VSFKARRGEVLGFAGLVGSGRTETMRLIFGADRAKSGTIKLGDKSVTIKSPVDAIRNG
                     VGLLTEDRKHQGLMLGLSIEDNITITHLKQFVLNHSELKETANRFVKDVNIKVSNIEN
                     PVSSLSGGNQQKVVLAKWLNADNQVYIFDEPTKGIDVGAKSEIYTIINQLALQGKTIL
                     IVSSEIPELLGICDRIQVFREGEITGEINVADYTNMKDEAQEVIMKYATLGQ"
     gene            6626..7594
                     /locus_tag="IW687_002013"
     CDS             6626..7594
                     /locus_tag="IW687_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951270.1"
                     /translation="MENEKKKLDIKSLFMNYSVYLVLIILVLYFSITNGDFLSSYNIE
                     NFLQQIPPVGILTVAYSMILICGNVDLSIGYVGMAAGMVGLYASNSGLPAPVVIAISL
                     VVGATLGTMNALLIKKFDLPSFILTLGTSYVIKGMLGFFTDEQFIGSEQEWFNKLAKT
                     PIGTDMVKSNALIFIIVIIIFVFVMKKTRLGRYTYAVGSNPEAARLSGINIDLHIVKI
                     FMIEGMLAAIAGLLIVSNLNGGASKDANGLDLFAMAAAIMGGTSFSGGIGTIGGAVAG
                     ILTLQVFKNGLTLMGVNSFLQDTVTGLVIIFAIIVDYFKRRASRQS"
     gene            7647..8699
                     /locus_tag="IW687_002014"
     CDS             7647..8699
                     /locus_tag="IW687_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0951271.1"
                     /translation="MFKKGKIILIGMLVAALFLTTGCSKGSSANGDAKKTLYFVPIVD
                     TGAYWNPMKKGAEDAAKDLGYTLVTKTSPSAEPSKKEKHIGFIKEATQKNVAGIAAAP
                     IEKKAFVDPIKAAVDKDIPVITFDADLENPKDRTAYVGTDNVSAGEELGKHAAEEMKA
                     KGITSGSIAVVCVDVAQPTMIDREKGMKKGFKEVYGEEAKNFKFLSTIQDNDQPSESK
                     KQLEGYISANKDLVTVFSLGSEGPDVGVMSALESQGKAGKILHYGFDYTKTWEKGIDD
                     GRITAIVDQDAYQIGYQVIENLVKAIDGEKIDSTIPIDVKYVKAEELKSYGKEKNKIK
                     TDSVDESEEDSTEDTK"
     gene            8772..10274
                     /gene="xylB"
                     /locus_tag="IW687_002015"
     CDS             8772..10274
                     /gene="xylB"
                     /locus_tag="IW687_002015"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAQ0951272.1"
                     /translation="MTYVLGLDLGTSALKGLVVGKNGQVVAEASHSYPMDTPKIGYSE
                     QNPLSWLEAADQVMKDLVSILPELKDSLEGISFSGQMHSLVLLDEKNTPLRPAILWND
                     VRTTKQCQKINETMGESILKREKNVALEGFTLPKILWVQENEPEVWKQVKQIQLPKDY
                     LRFWLTDKFSMDYSDAAGTLMLNQDSKKWDTELLRQWDIPEAILPQLVESAAVVGVMK
                     TELKERYGFSQEVKIIAGGADNACAALGSGVVKSGTGLCSIGTSGVFLKYEDKADRDY
                     QGKLHVFYHAIENTAYSMGVTLSAGNSLNWFKNTFAKDQPFEELLSEIDTVPIGCDGL
                     LFSPYIVGERTPYTDSQIRGSFIGIDTHHQLKDFTRSVLEGITLSLKDSQRIMEEVAE
                     EKIERLVSVGGGAKNKSWLQMQANIFNTPIVTLKTEQGPGLGAAMLAAVGCQWFLDLQ
                     ECADQFVAYKEEVQPQPQQVDAYKKVYDRYQKMYDATKDLCHLYFSQIEK"
     gene            complement(10488..10829)
                     /locus_tag="IW687_002016"
     CDS             complement(10488..10829)
                     /locus_tag="IW687_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0951273.1"
                     /translation="MTEEKNYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRK
                     TMFAVICPITSTIRNFPTRYSLPTELDTTGQILISQLKSLDFNERKLKKIESLPLQDM
                     AKIDQMIEYIF"
     gene            complement(10829..11059)
                     /locus_tag="IW687_002017"
     CDS             complement(10829..11059)
                     /locus_tag="IW687_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0951274.1"
                     /translation="MLITKSRLQGSSVVITLPSDNGKKPSENKEYIVIYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLSPEGREIL"
     gene            11291..11884
                     /locus_tag="IW687_002018"
     CDS             11291..11884
                     /locus_tag="IW687_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951275.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSTRPG
                     LETAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSLQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLTSSDLDLLKTLIDNG
                     TPIKTIAEKWNVSRTTIYRYLNKMEGKDNEQQKGSVS"
     gene            11856..13292
                     /locus_tag="IW687_002019"
     CDS             11856..13292
                     /locus_tag="IW687_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ0951276.1"
                     /translation="MNNRKDQLANYSDEQRHAAMAKYKMIKSNLEYKKSLAEVSEDEG
                     IPLRTLQRWKKHYLEEGLIGLVHKKRSDNGKIRLEPEVVSEIERLFFSYRKMSIATIQ
                     RKLCDFCSEKKFVPPSYSQVYKTIQSLPSSVGVLAHEGDKVYSEKFDLIKIREPKRPN
                     EVWQADHTLLDIEIIDTKGVPKRPWLTIIMDDYSRAIAGYSVSFDSPSAIHTALTLHQ
                     AIWTKRDSKWPICGIPEHFYTDHGSDFTSDHLEQVAIDLRINLLFSKVGVPRGRGKIE
                     RFFLTVNQLFLESLPGYIGNSDKTKRLNFQEFRNKLHHFLIYDYNLKEHSSIKMCPTD
                     KWNSKSFLPNLPESLEKLDLLLLEISKSRKVHSDGIHFQGLRYINPNLSAFVGESVLI
                     RYNPSDIAEIRVFYRNQFLCNAISPEISNYSIDIDDLIAARNKRKKILKEKIHSPSSI
                     DLLITEQEEKNTNSIRKKKSTLKRYINE"
     gene            13285..14097
                     /locus_tag="IW687_002020"
     CDS             13285..14097
                     /locus_tag="IW687_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0951277.1"
                     /translation="MNRTKKFIETKEYKRFAEFCDACIKYKYIGVCYGIPGVGKTVSA
                     RYYANWDSIFKQIDFKVNKEIGIHATKEILYANTIFCTAPAERQTKISSDIHTYGIRI
                     GMTRSIYLTEKEGKEDEGLSIDAYKGIDLIIVDEIDRLKLQHFEQLRDIYDQNDIAMV
                     LIGMPGIEKRLSRYPQLYSRIGFAHEFDNLSKDETHHIIEYKWSDLGLDLKLEDFSDY
                     EAITSIIKITKGNFRLLHRLFAQIHRILEINELNTITVDVVEAARVSLVIGL"
BASE COUNT         4554 a         2447 c         3065 g         4080 t
ORIGIN      
        1 cactttttta tttgaatgac gtgtattttt atagataaat ggcacatccg agtttagtag
       61 cctgactttc cattatgttg cttatctctc aaaattgttg atttatcaac ttcaaataat
      121 cgatcagttt ctttataggt ctgtgttttt acacaattgt tcaagtacta tggtaaaccg
      181 aggttcttca tcaccgataa agctccttcg attggtagtg catttaagaa attacctaat
      241 catggttttt atcagcatac agagtatcca accgtgaaat atctgaataa tctcattgaa
      301 taacaagacc aacgatcaat caagcgaaga tctaaattta tgtactgcta cgactacaaa
      361 taaaggtatg gaaatgattc gaggactata caagaagagc caaaaaaaga ctctcttttt
      421 ggcttttccg tgtgtcttga aattgaaaga ctattgggta ttccgttttt aatagattag
      481 agattaagta aatggtatcc cccctgtaaa aacctttgca acagaactga tattattgcg
      541 gacacagttg gaggatacca aatctattag ttaatggggc agttttaatt gattgatatt
      601 caaaaaatgc tgcaggttta gtacaaggcc gcctaaaaga gtcgcttttt cattgagaac
      661 cgtatttcta acaatgatct tatctgagaa gcggttggac aagtactgtt tcagatggtg
      721 aatcatttca ggaatctcac gataaagaga actgtttagc acaatgactt caggatcata
      781 atgagcaatc aagttgttga tgccgacact taagtaatgg acattttcct gaataatttg
      841 ttgtgcgtcc agatcgttat tttggtagta ttctcttaaa atgacagcat tcacgtaatc
      901 catggatttg atttttgaga acttttcaaa acaaaccttg tttgctgcat acatttctag
      961 acaaccgtga tttccgcagg gacatttttc cccgtctata acaatgattt ggtggccaat
     1021 ctcgccagca aatccatgag aaccaacttg cagctccccg ttgataacga tacctgctcc
     1081 gataccgcta tgcatattta cactgatcaa gctagaggaa ttagaggtga atgtgtattc
     1141 tcctaatgca gataagtttg cttcattttc taaatataca gggtaatcac tgatttcctc
     1201 aatgatctct cgaaaggcga tatgttccaa attgaggtga ggggtgaaaa tgagctcccc
     1261 gttataaaca ggcccatgaa cggcaacgac cacaccgata acaccgtaac ttgttttagg
     1321 caagttttcc tcaaatgatg caaatatttc agagcagtag gctttaactt ctttttcatc
     1381 taacatgatc ggtttctttt gaattatgcg gatttcttct ccatcgagat agcttaatag
     1441 agcattgatg taattataac caacatcaat ggagatcgag aggccggctt ttccgttata
     1501 tttcaaaata attggcttac gaccgccagc attgccagag ctaccgattc ctgtttccaa
     1561 gactaaatga tcctcaatta atttctttac aataacagaa atggtcgcct tagtgagacc
     1621 agtcaaatta gaaagatctg cgcgagacag gttttgatta ttcataataa gctcgaggca
     1681 caatgattca tttgtcgtgc gtcgcgcgta acttagattc ttcattttaa tacacgtccc
     1741 attaatttat taaataaact tactgctata gtatagcggt aaatttaatg ttttgctagt
     1801 tttttcgttg atttgtttat taaacatact taaaaaaacg gaaagtgtaa aactagtaat
     1861 accattcaaa atatttataa aagagcttga ctattcgtgt gaaagcggat actatatagt
     1921 caacaagtta gttagtatac aaaacgaacc aacttgaaag tgatgaactt taggaggaga
     1981 caagcatgag ttattttcca cgtgtagaaa aaatcaaata tgaaggtgtg aagacgaaga
     2041 atccatttgc gtttcgtcat tacaatccag aagaagtagt tgctggaaaa acaatgaaag
     2101 aacatttacg attcgccgtt gcctactggc acacaatgac ccaagacggc agcgatccat
     2161 tcggtttacc tacgaatcag cgtacttggt ttggtgctac agaaatggac accgctcgta
     2221 atcgagtaga ggcctttttt gaaattttag aaaaactagg tgcagagtac ttttgtttcc
     2281 atgatgtgga cattgctcca gaagggaaca gtttagcgga gttttataaa aatttagatg
     2341 agattactga tttaatcaaa gaaaagatgg atcaaacagg aatcaagttg ttatggaaca
     2401 cggcgaatat gttcagtcat ccaagatacg taaatggtgc tgcttctact aataatgctg
     2461 acgtctttgc ttacgctgct gcacaagtga aaaaaggctt ggacatttcg aaaaaattag
     2521 gcggtgaaaa ctatgtattt tggggtggac gtgaaggtta cgaaacctta ttgaatacag
     2581 atatggcctt tgaacaagac aacatcgcca gattgtttaa aatggcgatc tcatatggtg
     2641 aaaaaatcaa tcataagcca caattcttga ttgaaccaaa accaaaagaa ccaagcaaac
     2701 atcaatatga ttttgatgcg gcaacagcac tgcaatttat tcaaaaacac ggtttggaag
     2761 gcgaatttaa actgaacctt gaagccaatc acgcgaccct tgcagggcat accttcgaac
     2821 atgaaatcga agttgctcga accaacgatg cgttaggttc actggatgct aaccaaggag
     2881 atgttttact aggctgggat acagatgagt ttccaactaa cgtttatgat gtgaccttag
     2941 cgatgtatgg tgtattggaa aatggcggaa tcgcaccagg agggatcaac tttgactcaa
     3001 aagtacgtcg ctccagcttt gagatggagg atcttttctt agcgcatatt gctgggatgg
     3061 ataccttcgc tagaggctta aaagcagcgg cgaaattgaa agaagagcgt ttcttcgatg
     3121 atctgaagga aaaacgttat gccagtttca aagaaggaat cggcgcttcg attgtttccg
     3181 ataaagaaga tttggaaagc ttgacggatt atgcaatgaa gctgaaagat atccagctgg
     3241 aatctagtca tatcgaatat gtgaaatctt gtttaaatga ttatttagtg taaatgatac
     3301 gtgctctcta taatagaagc tcttgcagga aatgtggtgt tttcggttat agagagtatc
     3361 tcttaattta gtgaaaagga atggagctaa cgatgaaaat acagaatcca gttttaaagg
     3421 gctttaatgc tgatccctcg attgttcgtg ccggagatac ttattatatc gcaaattcga
     3481 catttgaatg gttccctgga gtccaaatcc atgagtcgaa ggatttggtt cattggcaat
     3541 tagtcacgcg tccattatca actattcgac tgcttgatat gaaaggtaac gctgactctg
     3601 gaggaatctg ggctcctgat ctatcatacg cggacggaaa attttggctt gtctatactg
     3661 acgtgaaagt agttgatggc gcatttaaag attgtaccaa ttatttgact acggctgagg
     3721 cgatcgaagg accatggagt gatccaattc atttaaatgg tgtgggattt gatgcatcgc
     3781 tttttcatga cgatgatgga aaaaaatacc tagtgcaaat ggaatgggat catcgagaat
     3841 accatcatgc atttaacgga ataaagtgca cagaatattc ggtggaagag caacgcttat
     3901 tacctgaaac ggcgaaaatt atttggcgag gtactgatgt gaaattagta gaaggtcccc
     3961 atctatataa attattcggc tattattatt tgttttgcgc agaaggtggc acgtcatatg
     4021 ctcatcagga agttgtttct cgctcaaaaa cactttacgg aacctacgaa agtcagcctg
     4081 aaaccttttt aacagctttt gatgcgcctg ataatgctct ccaaaagtgc ggacacggtt
     4141 cgctggttga gacgccaaac ggggagtggt attttgctca tttaactgga cgaccttggc
     4201 accattccta tgaatctgca cacgatcctc gcggctggtg cacattaggc agagaaaccg
     4261 caattcaaaa ggtgacttgg acaaatgaag gatggcctga gattgttggc ggtaaacagg
     4321 gaagtatggt agtcgatgca cctaaaggag cagagccgtt tgagtatgtg tctaacacgg
     4381 tagtcgatga ccagtttgac caagaggaat tgaacattca ttttcaaaca ctaagagtac
     4441 ctttcagtga tcgtatcggc aaaattgaaa acggtcagct gcacttgaat ggcttaggca
     4501 gtcttgcgaa tgttcatgaa aattctctag ttgcgagacg ctggcaggca tttgaatttg
     4561 cggcggaaac gaaaattcgc tataaaccga cgacatacca acaaatggca gggcttacca
     4621 actattataa ttcgacgcat tggtcgatga ttcagttgac ttggaatgaa gtgaacggtc
     4681 gcgtcattga agtaacggaa aataatcaag gagtattcaa aagctacttg aaagaagcgg
     4741 cgatctctgt tccagaagag atcaagtatg tgtatttcaa gacgatcgta tctacatcga
     4801 cgtattccta tgcgtactcc tttgatggtg aaagctggat agatatccct gttcaattag
     4861 atgcaaaagt attgtcggat gactacgtta attcaagcta cggcggattc tttacagggg
     4921 cattcgtggg aatggtgaat gttgattact caggctacaa ccttccagcg gtatttgatt
     4981 atttccggta tcaagaagtt tagttccttg ctgccaattg cttatttttt caaaaaatag
     5041 aaggaggttg tcatggagaa cgagctttta ttttcaatga cagatattga aaaaagtttt
     5101 ggtcctgttc aagtactgaa aaaagcaaac cttgaagttc gaaaaggtga gatccatgct
     5161 tttatgggag agaacggagc agggaaatcc accttaatga acattctggc tggcttgatc
     5221 gataaagatg ctgggaaaat tatttttgaa ggaaaagaaa ttccaaaaat gacgacggac
     5281 ttagcaacag agttagggat cggatttgtt catcaagaac taaatctagc agaggattta
     5341 tctgtggctg aaaatgttta tattggccga ttgccttata aaaataagcc ccttggaatc
     5401 gttgatttta agaagctgca tgaggatacg atgtattggc tcaaccttgt cggtgctagt
     5461 gtgagtcccg atgcacatgt aggctctctt tctactgcta ataagcagct gattgagatt
     5521 gctaaagctc ttagtttaaa tgctaaggtg attatttttg atgaaccaac gacttcttta
     5581 tcggataaag acgttgaagt attattcatc atcattcggc gattaaagga aaagggtttt
     5641 tcgagtattt atatttcaca ccgcatgaag gaaatttttg aacttggcga acgcatcact
     5701 atcatgcgag atggcaaata tatcacgacc gatgacgtga aagatttgtc agaagatcag
     5761 atcatttcaa tgctggtggg acgtgagatc aacgacttat atccgaagca ggcaacagag
     5821 attggtgaga cgtatttgga agttgaccag ctctctgacc gctttggtca tgtaaaagat
     5881 gtcagcttta aagcaagacg aggagaggtt ttaggctttg cgggtctagt tggcagcgga
     5941 aggaccgaga cgatgcggct gatttttggc gcggatcgtg ccaagtctgg aaccattaag
     6001 ctgggcgata aatcagtgac cataaaatca ccggtggacg cgattcgcaa tggagtagga
     6061 cttttgacag aagatagaaa gcatcaagga ttaatgctgg gactgtccat cgaagacaat
     6121 atcacgatta cccacttgaa acagttcgtg ttgaaccatt cggagttgaa ggaaacggca
     6181 aatcgcttcg tgaaagatgt aaacataaag gtcagcaaca ttgaaaatcc cgtatcaagt
     6241 ctctctggtg ggaatcagca gaaggtagtt ttagctaaat ggctaaatgc cgataaccaa
     6301 gtctacattt ttgacgagcc cacaaaagga attgacgtag gggcgaaatc agagatatac
     6361 acgataatca atcagttggc attgcagggc aagacgattt tgattgtttc ttctgaaatt
     6421 ccagaattat taggaatttg tgatcgtatc caagtcttta gagaaggaga aatcactggt
     6481 gaaataaatg ttgctgatta tacgaatatg aaagatgaag cgcaggaagt cattatgaag
     6541 tacgcaacgc tgggccagta attcaaaaag atttgcttaa ggtcacattt tgtttgtact
     6601 actacggaat gaaggagata aaacaatgga aaacgaaaaa aagaagctag atatcaaatc
     6661 tctttttatg aattattcgg tctacctagt gttaataatt ttagtacttt atttttcaat
     6721 tacaaatgga gattttctaa gctcctataa tatcgaaaac tttttgcagc aaattccacc
     6781 tgtggggatt ttgaccgttg cttattcgat gattttgatc tgtggaaacg tagatttatc
     6841 aattggatat gtagggatgg ccgcggggat ggtggggctt tacgcctcca atagcggctt
     6901 gccggcacca gttgtaatcg ctatctcatt agtggtcgga gcgaccttag gaacgatgaa
     6961 tgcccttttg atcaaaaaat ttgatttgcc gtcgtttatt ttgacgctgg gcacaagcta
     7021 tgtaatcaaa gggatgcttg gctttttcac tgatgaacag tttatcggtt cagaacaaga
     7081 atggttcaac aagctggcaa aaacgccaat tggaacagat atggtcaaaa gcaatgcatt
     7141 gatcttcatt atcgtgatta tcatttttgt ctttgtcatg aaaaaaacac ggctaggcag
     7201 atatacctat gcggtaggtt caaatccaga agctgcacgg ttatcaggga tcaacatcga
     7261 tttgcacatt gtcaaaatat tcatgattga aggcatgttg gcggcgatcg caggcttgct
     7321 gattgtttct aacctgaatg gaggcgcttc gaaagacgcg aacggtttag acttgtttgc
     7381 gatggcagcc gcaatcatgg gaggaacgtc atttagcggc gggatcggta cgataggtgg
     7441 cgcggtcgca gggattttga cattacaagt atttaaaaat ggcttgacac ttatgggagt
     7501 taactcattc ttacaagata ctgtgacagg tttggtcatt atttttgcga tcatcgtcga
     7561 ctacttcaaa cgtagagcca gccgacaatc gtagaaatca aaatagtctt attaaaaatg
     7621 tgaagcagag aaaggaatgt gcaaaaatgt ttaaaaaagg aaagattata ctgattggca
     7681 tgttagtcgc ggcattattt ttaactacgg gatgtagcaa gggaagctcc gcaaatggag
     7741 acgctaaaaa gacattgtat tttgttccga ttgttgatac aggcgcctat tggaacccga
     7801 tgaaaaaagg ggcggaagac gcagcgaagg atttggggta tacattagta acgaaaacat
     7861 ccccatctgc agaaccgagt aaaaaagaaa agcacatcgg cttcattaaa gaagctacac
     7921 aaaagaatgt cgctgggatc gcagctgcac ctatcgaaaa aaaggccttc gtagatccga
     7981 tcaaggccgc agtcgataaa gatatcccag tcattacttt tgatgcggat ttagagaatc
     8041 ctaaagatcg aacggcttat gtaggaacgg ataatgtctc tgccggtgaa gaactaggaa
     8101 aacacgcagc agaagaaatg aaagcaaaag gcatcacgag cggttcaatc gcggtcgtgt
     8161 gtgtggacgt agcgcagccg actatgattg atcgggaaaa gggaatgaaa aaaggcttta
     8221 aagaagtgta tggcgaagag gctaaaaact tcaagttcct atctactatt caagacaatg
     8281 accaaccctc tgaatcaaag aaacagctag aaggatatat ttctgcaaat aaagatttag
     8341 taacggtatt ctcattaggc tctgaaggtc ctgacgtagg agttatgagt gctcttgagt
     8401 cccaaggaaa agccggtaag attctccact atggatttga ttatacaaaa acgtgggaaa
     8461 aagggatcga cgatggacgt attactgcta ttgttgacca agacgcttac caaatcggct
     8521 accaagtcat tgaaaatctt gtcaaagcaa tcgatggaga aaaaatcgat tcgacgattc
     8581 caattgacgt aaaatatgtc aaggcagaag agttgaagag ttacggcaag gaaaagaata
     8641 agatcaaaac agattctgtg gatgaatccg aagaagacag tacagaggat acgaaataaa
     8701 cgaatgagca tagtaatggg agatgaagga gagcttgtct cccattactt ctttaacgga
     8761 gaggtgagaa gatgacttac gtattaggct tggacttagg aacaagcgct cttaaagggc
     8821 tggtagtcgg taagaacgga caagtggtgg cagaagcatc tcattcttat ccaatggata
     8881 caccaaaaat tggatacagt gaacaaaatc cgctttcctg gctcgaggcg gcggatcagg
     8941 tgatgaagga tctcgtttcc atacttccag agctgaaaga ttcgttagaa ggaatcagct
     9001 tttcaggaca gatgcatagt ttggtacttt tagatgaaaa gaatactcct ttacgaccag
     9061 caattttgtg gaatgacgtt cgtacgacga agcaatgcca aaaaataaat gaaacaatgg
     9121 gtgaatccat cctaaaacgg gaaaaaaatg ttgctttaga aggattcacc ttgcccaaaa
     9181 ttttgtgggt gcaggaaaat gaacctgaag tctggaaaca agtaaagcag attcagctgc
     9241 ctaaggacta tttgcgtttt tggctcacag ataaattttc aatggattat tcggatgcgg
     9301 cgggtacatt gatgctaaat caggatagca aaaaatggga tacagagctg ttaaggcagt
     9361 gggatattcc agaagctatt ttacctcaac tggtagaatc agctgctgtc gtcggagtga
     9421 tgaagacaga attgaaagaa cgctatggat tttcacagga ggtcaagatt attgccggag
     9481 gagcggacaa tgcctgtgcg gctctgggtt caggtgtggt caaatccgga acaggcctgt
     9541 gttccatcgg tacaagcgga gtttttctta agtatgaaga caaggctgat cgagactatc
     9601 aaggcaagct gcatgtcttt taccatgcaa tagaaaatac ggcttattca atgggggtta
     9661 ctttatcagc gggaaatagc ttgaattggt tcaagaacac ctttgccaaa gatcagccat
     9721 ttgaagaatt gctgtctgag attgacaccg tgccgatcgg ctgcgacggc ttgctttttt
     9781 ctccatatat cgttggggaa cgtacccctt acaccgatag tcaaatcaga ggaagcttca
     9841 ttggcatcga tacccatcat cagctaaagg atttcactcg cagtgtgtta gaaggaatca
     9901 ctctctcctt aaaggattcc cagcgaatca tggaggaagt ggcagaggaa aaaatagaaa
     9961 ggctagtatc tgtaggcgga ggtgcgaaaa ataaaagttg gctgcaaatg caggcaaata
    10021 tttttaacac gccgatcgtc acattgaaaa cggaacaagg gccaggatta ggagcagcca
    10081 tgctggctgc tgtaggctgt caatggtttt tagacttaca agaatgtgcg gatcaatttg
    10141 tcgcgtacaa agaggaggtt caacctcagc ctcaacaagt agatgcatat aaaaaggtgt
    10201 atgaccggta tcagaaaatg tatgatgcga ctaaggatct ttgccatctt tacttttctc
    10261 aaatagaaaa ataaagaata tatcattgat tggttttctc gagatgtgaa tggaatgggc
    10321 cgttgattga agcggaattt aatttgggat tccaatagaa attataattt gattaatgaa
    10381 ctttcctatg atgttaaaaa ttgattttag aatcgtaaat atacaaaaaa ataaagtaat
    10441 gaaccattac atttttacta atgtgacggt tcattacctt acattaatta aaatatatat
    10501 tcaatcattt gatcgatttt cgccatatct tgtaaaggta aggattcaat ttttttaagt
    10561 tttctctcat tgaaatcgag cgacttcaat tgagaaataa gtatttgtcc agtagtgtct
    10621 aattcggtag gtaatgaata gcgtgtcgga aaatttctta tagtagaggt aatcggacaa
    10681 ataactgcaa acattgtttt gcgattaaac tcgtatctag aaacaactaa tccaggacgc
    10741 cgcttttgaa tctcttttcc tgcagaagga tcaaaatcaa tccaaacaat atctcccttt
    10801 ttaggaatgt aatttttttc ttcagtcatt ataaaatctc tcttccttca ggagacaaat
    10861 cttcccattc atctttttcg taatactccg cttcttctcc accactaaat ggatcttcaa
    10921 tttttggaac caaagtaatt gttccatcat cagaatagat aactatatat tctttatttt
    10981 ccgaaggctt ctttccgtta tcagaaggca aagtaatcac tacagaactt ccttgtaatc
    11041 tagattttgt aataagcata gtgccacctc ttttcatcta ttttatcatg caacacttaa
    11101 tcacacaaca tatatttatt ctagtttaaa gtgtccagaa aatgaccctt ttctgtacac
    11161 tttctttttt caatctattt ttactagaat gaatcttttt cgtgttcatt aactcgtatc
    11221 aaaatctatg ttcaattaat ccttatagat aaagttatgg tacaatggtt aacgaaaagg
    11281 agcgattttt ttgaaaatag gatatgctcg ggtttccacc ggattgcaaa atttagattt
    11341 acaagaagat agtttaaata aatttggttg tgagaaaatt ttcactgatc atatgagtgg
    11401 cgcaaaaagt accagacctg gattagaaac agcaattgat tttgttcgtt cgggtgatac
    11461 tttggttgta tggcgattgg accgactagg aagaaatatg gaagatctga tttcgattgt
    11521 caatcgattg aacgaacgtg gagtcagttt ccatagtcta caagaaaata ttacgatgga
    11581 taaatctagc tctaccggtc aattgatgtt tcatttgttt gcagcgtttg cagaatttga
    11641 gcgtaatttg attttagagc gttctgcagc tggtcgtgca gcagcaagag caagaggtcg
    11701 ctttggaggt cgcccagaaa aattaacatc aagtgacttg gatttactga aaacattaat
    11761 tgataacgga acgccaatta aaactattgc ggaaaaatgg aacgtctctc ggacaacaat
    11821 ttatcggtat ctaaataaaa tggagggcaa agataatgaa caacagaaag gatcagttag
    11881 ctaattattc cgacgaacaa agacatgcag caatggccaa gtacaaaatg atcaaatcca
    11941 atttagagta taagaaatcg ttagctgaag tttctgaaga tgaaggaatc ccactccgga
    12001 cattacaacg ctggaagaaa cactatctag aagaaggatt gataggtcta gttcacaaaa
    12061 agagatctga taatgggaaa atccgtttag aaccagaagt tgtcagtgaa attgaaaggc
    12121 tgttcttcag ttatagaaaa atgagtattg caactattca aagaaagcta tgtgactttt
    12181 gttccgaaaa gaagtttgtt ccgcctagtt atagtcaggt ctacaaaacg attcagtctc
    12241 tcccctcttc tgtaggagta ctggctcatg agggtgataa ggtttattca gaaaagttcg
    12301 accttatcaa aattcgagag cctaaacggc caaatgaagt atggcaagcg gaccatactt
    12361 tattagatat tgagatcatt gatactaaag gagttccgaa acgtccatgg ttgacgatta
    12421 tcatggatga ttacagccgt gcaattgccg gctattctgt atcctttgat tccccttctg
    12481 ctattcatac cgcattaaca ttgcaccagg ctatttggac caaacgagat tccaaatggc
    12541 ctatttgtgg tatcccagaa cacttttata cagaccatgg gagtgacttc acttcagatc
    12601 acttggaaca agttgcgatt gatctgagaa ttaacctatt gttttctaaa gtaggcgttc
    12661 cacgtggtcg aggaaaaatc gaacgattct ttctcacagt taatcaactc ttcttagagt
    12721 cattaccagg atatattgga aattctgata agacaaaacg acttaatttt caagaatttc
    12781 ggaataaact tcatcatttt ttgatctatg actataacct taaagaacat tcttcaatca
    12841 agatgtgtcc aaccgataaa tggaattcaa aatctttttt accgaacctt cctgaaagcc
    12901 ttgaaaaatt agacttatta ttgctggaaa tttctaagtc gagaaaagtg cattcagatg
    12961 gtattcattt tcaagggctg agatatatca accccaacct ctctgctttt gtaggagagt
    13021 cggtcctgat tcgatacaat ccctcggaca tagctgaaat tcgagtgttt tatcgtaatc
    13081 aatttttatg taacgcaata tctcccgaga tatcaaatta ttcgattgat atagatgatt
    13141 tgattgcagc aaggaataaa cgcaaaaaga tactgaagga aaaaattcat tctccaagct
    13201 caatcgatct cttaattaca gagcaagagg agaaaaatac taattctatt agaaagaaaa
    13261 aatcaactct aaagaggtat atcaatgaat aggacgaaaa aatttattga gacaaaggaa
    13321 tacaaacgat ttgcggagtt ttgtgacgca tgcataaagt ataagtatat tggtgtttgt
    13381 tatggtatac ctggagttgg aaagactgtt tcagcacgtt attatgctaa ctgggatagt
    13441 atttttaaac aaatagattt caaagtcaat aaagaaatcg ggatacatgc aacaaaagaa
    13501 attctttacg ccaatacgat tttttgcaca gcaccagcag aaagacaaac taagattagc
    13561 agtgatattc atacttatgg aattcggatt gggatgactc gtagtattta tcttaccgaa
    13621 aaagaaggaa aggaagatga aggtttatca atcgacgcgt ataagggaat tgatttaatt
    13681 atcgttgacg aaattgaccg attaaagctt caacatttcg agcaattaag agatatttat
    13741 gatcaaaatg atattgcaat ggtcttgatc ggtatgcctg gtatagaaaa acgattaagt
    13801 agatatcctc aactctattc aagaatcgga tttgcacatg agtttgataa ccttagtaaa
    13861 gatgaaactc atcatatcat tgaatataag tggtctgatc ttggattgga tcttaaactt
    13921 gaagatttct cagactacga agctataact agtatcataa aaattacgaa aggtaacttt
    13981 agattgctgc accgcttatt tgctcaaatc catcgaatat tggagattaa tgaactaaat
    14041 actattaccg tagatgtagt agaagcagca agagttagct tagtcattgg cttgtaaata
    14101 cgtcatttat atttaaagaa aaacgctatt taaatataaa agtcac
//
LOCUS       DACELF010000069        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.072, whole genome shotgun sequence.
ACCESSION   DACELF010000069 DACELF010000000
VERSION     DACELF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.072"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW687_002021"
     CDS             131..505
                     /locus_tag="IW687_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0951278.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW687_002022"
     CDS             complement(619..1248)
                     /locus_tag="IW687_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0951279.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW687_002023"
     tRNA            complement(1382..1454)
                     /locus_tag="IW687_002023"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IW687_002024"
     tRNA            1668..1741
                     /locus_tag="IW687_002024"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IW687_002025"
     CDS             1696..1845
                     /locus_tag="IW687_002025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951280.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IW687_002026"
     CDS             2338..3666
                     /locus_tag="IW687_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0951281.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IW687_002027"
     CDS             3868..4701
                     /locus_tag="IW687_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0951282.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IW687_002028"
     CDS             4717..5454
                     /locus_tag="IW687_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0951283.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IW687_002029"
     CDS             complement(5609..6577)
                     /locus_tag="IW687_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0951284.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKKRILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IW687_002030"
     CDS             6809..7642
                     /locus_tag="IW687_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0951285.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IW687_002031"
     CDS             7676..8515
                     /locus_tag="IW687_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0951286.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IW687_002032"
     CDS             8541..9101
                     /locus_tag="IW687_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0951287.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IW687_002033"
     CDS             9300..11021
                     /locus_tag="IW687_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0951288.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IW687_002034"
     CDS             11186..12328
                     /locus_tag="IW687_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0951289.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IW687_002035"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IW687_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0951290.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2297 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttttttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACELF010000070        13870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.073, whole genome shotgun sequence.
ACCESSION   DACELF010000070 DACELF010000000
VERSION     DACELF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.073"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..662)
                     /locus_tag="IW687_002036"
     CDS             complement(6..662)
                     /locus_tag="IW687_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0951291.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            855..1133
                     /locus_tag="IW687_002037"
     CDS             855..1133
                     /locus_tag="IW687_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951292.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1152..2360
                     /locus_tag="IW687_002038"
     CDS             1152..2360
                     /locus_tag="IW687_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0951293.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2508..2873)
                     /locus_tag="IW687_002039"
     CDS             complement(2508..2873)
                     /locus_tag="IW687_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0951294.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3020..3793)
                     /locus_tag="IW687_002040"
     CDS             complement(3020..3793)
                     /locus_tag="IW687_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0951295.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3967..4827)
                     /locus_tag="IW687_002041"
     CDS             complement(3967..4827)
                     /locus_tag="IW687_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0951296.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4827..6245)
                     /locus_tag="IW687_002042"
     CDS             complement(4827..6245)
                     /locus_tag="IW687_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951297.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6302..6751)
                     /locus_tag="IW687_002043"
     CDS             complement(6302..6751)
                     /locus_tag="IW687_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0951298.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7196..7459)
                     /locus_tag="IW687_002044"
     CDS             complement(7196..7459)
                     /locus_tag="IW687_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0951299.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7558..8853)
                     /locus_tag="IW687_002045"
     CDS             complement(7558..8853)
                     /locus_tag="IW687_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0951300.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8850..10121)
                     /locus_tag="IW687_002046"
     CDS             complement(8850..10121)
                     /locus_tag="IW687_002046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0951301.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10286..11155)
                     /locus_tag="IW687_002047"
     CDS             complement(10286..11155)
                     /locus_tag="IW687_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0951302.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11447..13057)
                     /locus_tag="IW687_002048"
     CDS             complement(11447..13057)
                     /locus_tag="IW687_002048"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0951303.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3948 a         2920 c         2367 g         4635 t
ORIGIN      
        1 gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg
       61 tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta
      121 gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc
      181 atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca
      241 gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga
      301 tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa
      361 aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg aatattatac
      421 ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg
      481 atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc
      541 tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat
      601 cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc
      661 aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt
      721 taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac
      781 ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa
      841 tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat
      901 atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact
      961 cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa
     1021 atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca
     1081 attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat
     1141 gtataacata catggaaaag aaattattta ataaatattt tattggaatc acattgatca
     1201 attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag
     1261 acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa
     1321 cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct
     1381 taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta
     1441 tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag
     1501 ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt
     1561 attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc
     1621 tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta
     1681 cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat
     1741 ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa
     1801 ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa
     1861 aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca
     1921 catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt
     1981 atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg
     2041 tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg
     2101 gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct
     2161 actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat
     2221 ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag
     2281 cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat
     2341 cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa
     2401 taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt
     2461 tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc
     2521 cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca
     2581 ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa
     2641 tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg
     2701 ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt
     2761 accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac
     2821 agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc
     2881 actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat
     2941 accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt
     3001 agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg
     3061 catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa
     3121 tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt
     3181 ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc
     3241 cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg
     3301 catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca
     3361 atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg
     3421 taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac
     3481 tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct
     3541 tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt
     3601 ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca
     3661 gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg
     3721 tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca
     3781 cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt
     3841 gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac
     3901 atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt
     3961 ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac
     4021 cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg
     4081 attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa
     4141 attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga
     4201 accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat
     4261 atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc
     4321 aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat
     4381 gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc
     4441 cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc
     4501 gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg
     4561 taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt
     4621 ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg
     4681 atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat
     4741 catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt
     4801 caataactct ttcattgtat ataacattac gcttccacct catttgttag attttcattt
     4861 ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag
     4921 gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca
     4981 tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc
     5041 atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg
     5101 tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat
     5161 gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa
     5221 gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact
     5281 ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata
     5341 tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag
     5401 ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat
     5461 accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata
     5521 gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct
     5581 agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg
     5641 gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct
     5701 aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct
     5761 aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt
     5821 gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa
     5881 tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg
     5941 aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg
     6001 cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct
     6061 actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt
     6121 tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat
     6181 ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt
     6241 ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt
     6301 ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct
     6361 ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat
     6421 ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc
     6481 cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt
     6541 tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc
     6601 gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca
     6661 catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca
     6721 gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta
     6781 acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa
     6841 tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc
     6901 tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc
     6961 ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat
     7021 aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca
     7081 ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa
     7141 aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt
     7201 ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg
     7261 cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac
     7321 ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg
     7381 aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg
     7441 gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag
     7501 agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta
     7561 tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa
     7621 aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa
     7681 tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat
     7741 caataattct tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg
     7801 ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata
     7861 gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact
     7921 gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc
     7981 tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg
     8041 agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg
     8101 cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa
     8161 tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg
     8221 ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc
     8281 gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg
     8341 accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt
     8401 ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc
     8461 ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg
     8521 ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa
     8581 tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg
     8641 ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa
     8701 tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt
     8761 actgtaataa ggaattttaa attctttggc ataactataa acttctttta acgttttgtt
     8821 ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca
     8881 attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa
     8941 tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca
     9001 cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg
     9061 atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg
     9121 ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt
     9181 aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg
     9241 tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata
     9301 tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt
     9361 ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg
     9421 cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc
     9481 acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct
     9541 atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca
     9601 gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca
     9661 cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca
     9721 cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca
     9781 cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa
     9841 gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga
     9901 tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca
     9961 attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct
    10021 aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca
    10081 ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat
    10141 taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg
    10201 gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta
    10261 tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat
    10321 gagatttagc atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga
    10381 atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg
    10441 ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga
    10501 tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag
    10561 catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac
    10621 catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc
    10681 attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg
    10741 aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag
    10801 ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc
    10861 ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg
    10921 gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac
    10981 cacccatgta tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt
    11041 cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag
    11101 cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa
    11161 aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta
    11221 ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa
    11281 gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc
    11341 tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc
    11401 ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt
    11461 gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag
    11521 aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga
    11581 tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca
    11641 attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg
    11701 ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa
    11761 ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat
    11821 tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac
    11881 gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat
    11941 tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac
    12001 caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt
    12061 cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca
    12121 atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa
    12181 tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt
    12241 ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt
    12301 gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg
    12361 gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg
    12421 tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct
    12481 gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga
    12541 tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg
    12601 gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag
    12661 tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt
    12721 gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt
    12781 ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc
    12841 ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg
    12901 ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta
    12961 agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg
    13021 aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat
    13081 ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg
    13141 gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa
    13201 gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt
    13261 tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc
    13321 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
    13381 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
    13441 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
    13501 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
    13561 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
    13621 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
    13681 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt
    13741 ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca
    13801 tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc
    13861 tctggataaa
//
LOCUS       DACELF010000071        13576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.074, whole genome shotgun sequence.
ACCESSION   DACELF010000071 DACELF010000000
VERSION     DACELF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.074"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..503)
                     /locus_tag="IW687_002049"
     CDS             complement(<1..503)
                     /locus_tag="IW687_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951304.1"
                     /translation="MCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRG
                     NVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQL
                     TVRDRIAG"
     gene            complement(1070..2005)
                     /gene="whiA"
                     /locus_tag="IW687_002050"
     CDS             complement(1070..2005)
                     /gene="whiA"
                     /locus_tag="IW687_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0951305.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2039..3037)
                     /locus_tag="IW687_002051"
     CDS             complement(2039..3037)
                     /locus_tag="IW687_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0951306.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3034..3918)
                     /gene="rapZ"
                     /locus_tag="IW687_002052"
     CDS             complement(3034..3918)
                     /gene="rapZ"
                     /locus_tag="IW687_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0951307.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4053..4784)
                     /locus_tag="IW687_002053"
     CDS             complement(4053..4784)
                     /locus_tag="IW687_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0951308.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(4788..6053)
                     /locus_tag="IW687_002054"
     CDS             complement(4788..6053)
                     /locus_tag="IW687_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0951309.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6316..9132)
                     /gene="uvrA"
                     /locus_tag="IW687_002055"
     CDS             complement(6316..9132)
                     /gene="uvrA"
                     /locus_tag="IW687_002055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0951310.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9142..11136)
                     /gene="uvrB"
                     /locus_tag="IW687_002056"
     CDS             complement(9142..11136)
                     /gene="uvrB"
                     /locus_tag="IW687_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0951311.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11395..12897
                     /locus_tag="IW687_002057"
     CDS             11395..12897
                     /locus_tag="IW687_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0951312.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12899..>13576
                     /locus_tag="IW687_002058"
     CDS             12899..>13576
                     /locus_tag="IW687_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951313.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQI"
BASE COUNT         3908 a         2936 c         2338 g         4394 t
ORIGIN      
        1 cctgcaatac gatcacggac tgtgagctgt ggcttgcttc caccatcatt cataaaatat
       61 ccagcagagc cactacttaa agtgattgtt acatcttcac tttctattat taagaattca
      121 taatgaaatg ctttcaactc tccactatta tttaaaagtt ttatactcca acttcgtcta
      181 ggttgagttg tcacattccc tctaaaactt cctataaatt ctgcaatcac cgtatcttca
      241 atcaaaattc tttcctcatc tatttcatat tcaagactaa acggaaattc tccatatgta
      301 atctctggca atggtggtgc tgtagttctc caaataaata caataactgt gaatagaaaa
      361 aatccaacga tcgatataaa tgttatgaga cctgctcgaa gtaaaacccg atataataaa
      421 aatgctgtat ttcgctttgc tatatctaga aaaataaaaa gtactgcaat cataatgata
      481 gtaaaagttg taaaaaagca cattttttgc cctcccttta taaacatcga ttctcaaatt
      541 ctaaaccaaa tcttgatagt tcaagttcag gtataacgta taccaaccat acatcccaat
      601 tactatttat gaacttatga ttatcactga ataaatcact ccatcccctt gttaaaactt
      661 ctacattaat tacacctggt tgttttcgac tagctaatcc aatgaactta tcaaccatcc
      721 ttatccacca accacgagaa tcttgaccag aagtaagaaa aacagttctc aaggttgatc
      781 gagcagttcc ttccagtttt taattaatct ccagttatag ataaatactt gatcctatct
      841 atatcaggac ttctatatta agcaaaagtt tatgtataaa actaaaatct tcattcagtt
      901 gtctccccgc tttttatacg attgtattaa aaaacaatag aagcctttca acattatgtt
      961 actccagtaa agttggaatt atagtttgac ggaagaaaag caagtatagc ttttaaaaaa
     1021 aactgataca ttcgcatctc ctgcttcatg tatcagtttt ttctttgaat taaactgctt
     1081 cttcacgcag tttttccgca aattcattta ttttgcgaat tcggtgatta attcccgatt
     1141 ttgatattgc acctgaggga atcatttccc ctaattcttt caagctgacc tctggatgtt
     1201 gaagccgaag ttctgcgatt tcctgaagtt tttcaggaag ggcatgtaga ccgacctttg
     1261 cttcgatcaa ttcgatgttt tcgatttgtt tagaagccgc atcaatcgtc ttgttcagat
     1321 ttgctgtctc acaattgact agacgattga ccgaatttct catatcccgt acgattcgaa
     1381 catcttcaaa tcggagcatt gagttggttg caccaatcaa tgttaagaaa tcagcaattt
     1441 tttctgctcc ttttaaatac gaaatatagc cattgcgccg ttcaagcgtt cgagcatgta
     1501 gaccgtaata atttaacata tcgcaaatgt cttggttatg ctgttcataa atagaaaata
     1561 tttccaaatg ataacgactc gtttccggat tattgactga acctgaggcc ataaaagcac
     1621 cacgtaagta agaacgcatt ttttgcgaat ttcccataat ctcgtccgaa acgtgtccat
     1681 tgaacatcat accatccatg atatcaagat ccatcaacac atcttttgtg ccttgcttca
     1741 accggacgat atagacattg ttctttttta atttcatttt tttacgaacg agtagctcac
     1801 ttcgtacctg ataatgatct ttcaaaagag aatagatacg tctggcaatt gcagcatttt
     1861 ctgtttggac gttcaaaata aactgctggt tcacaatacc taaagagccg ttcattcgca
     1921 gcaaagcagc taattccgct ttcgcatgtt ctcgatgaac ttccaaacca gttagttctt
     1981 tcttcacatc tgcagcaaat gacatcctat tctcctcctt tctccaacaa taaattgctt
     2041 aatattttga accgaaaact aaacggaaca attcttctac tactttgtct ccatcgtgga
     2101 agacaccacc atctttcaat tctaaaaagt cggtggaaat cactcgacat ccttcatttc
     2161 tcaatccgtt gaagtcgtgt gcgacttgta ctagatactc atcatagatc tctgggtcca
     2221 tataatcttc aggcactttt tccgtattga ccaatactgt atcgataaaa ggctgattca
     2281 aatgcttgtg taaaacacgt atatgatcgg catctgtgaa atgttccgtt tccccttttt
     2341 gggtcatgat attacagata tagacggttt cagcagttgt cttgagcatt gcttcgccga
     2401 tttctggtat cactaaattt ggaaggatac tggtgaataa acttccgggt cccaaaacga
     2461 ccatgtcggc gtccataatt gctgaaacga cttttcgagc cgctttggct tcacttttat
     2521 catttgtgtt gcagacatac acacggtcaa tggttttacg gttgatcgcg attttagatt
     2581 ctcctactgc agtcgttccg tctttaaaaa cagcatgcaa ggtcaaagga gtttctgaag
     2641 aaggataaac atgtccatct acatgcatca tttttgctaa taattgaatc gcctcataag
     2701 tgcttccccg catctcggaa actgcggcaa tgatcagatt accgatcgta tgattggcta
     2761 aaaactgatc ttcctttttg aaacggtatt ggaaaatatc ttcatagaat tgtggcatat
     2821 ccgataacgc cactaagaca tttctcaagt caccaggagg agccatagcg atcgattctc
     2881 tgattgcacc gctgcttcca ccatcatctg caacagtcac tactgctgtg atatctgctc
     2941 cttgatttcg taaacttttc aggataactg gcagaccagt gccaccgccg accactacga
     3001 tctttggttt tcttatccgg taggttttca ttttcacgac cggttcaccg tctctttcct
     3061 tttatccttg tcccgatgtg tcacattgac tttatagttt ttccctaaag catttgccaa
     3121 acgttcagtc aatgccacag aacggtgttg accgcctgta cagccgattg caactgtcaa
     3181 gctgcttttt ccttctttaa tgtatccagg caatacagtt tccagaagat ccaaaaattt
     3241 atggtagaat gattgcgttt catcaaagga catcacataa tcatagactg gctgatcttt
     3301 tccggtcaat ggtcttagtt ccgggatata atgagggttg ggcaaaaaac gcacatccat
     3361 cacaatatcc gaatcgatcg gcaacccgta tttaaaacca aatgatacaa attcgatccg
     3421 aaatccttgg tctccctttg atttgaatga ctcattgatt ttttcacgta attgtcgtgg
     3481 tgtcaatgtt gtcgtatcaa tgatcacgga agcttgggct ttcaagtcgt ctagtatcgc
     3541 ccgttcttta cgtatccctt cagtgatcaa accgtccatc gccattggat gtgctcgtct
     3601 tgtttctttg tagcgagcca ctagttcttc gtctgaagca tctaaaaata agatagaagt
     3661 gtcgatgaag ttcgtatttt cgatttcgat cagcatgttt tggatttctt caaagaagga
     3721 acgagaacgc aaatccactc ctaaagccat ttttgtgact ttacctgatt ctttgatcaa
     3781 ttcccagaat tttggaatca atcgaggcgg catattatcg atacaaaaat agcccatatc
     3841 ttcaaaactt tggatagcta ctgttttccc tgcaccactc atacctgtta tgatgactaa
     3901 ttgcaaatta tctgtcatat ttacgaaccc ctctcacatt gtgcataata aaattatatc
     3961 atcccgggcg tatcattacc agttttcttt ttcttttaca aaaagatttg gaccaaatat
     4021 cttttaggat atttgcccca aatccaattt ttttactttt ccgatgctgt atccaatgcc
     4081 tcgcttcttt cagctaatgc acgttcgatc gttcgatccg ctaaaataga ttgagcatcg
     4141 atcactgggt aatgattatc ttcagcaaat tcatgcatca aagataattc tgtgcaacct
     4201 aggatgactt tttcacagtt taggcgctct accgcttcct ctaagatttc gtaatagagc
     4261 tcttgattca aataatccga ttccttgatc tcatgataga tcaggtaatt gattttttct
     4321 tgaagcgcag tatcaggaat cactgtttcg aaaccaagat ttttgacttc gcgttcatag
     4381 attccagctt tcatacttcc ttctgttcct agaatcgcta ctctccctgt cgtatgctga
     4441 cggacaagtt catttgctgc ttctcttggc atatgcaaga taggaatatc cgttgccgct
     4501 tgtaactctt caaaaaagta gtgtgcggtg ttacaagtca acacaatgaa gtttggtcgc
     4561 aataaatttt gtttctcaat atcatccagc agaaacggca ttggattttc ttctgatcga
     4621 tctaaaatat aagctgtacg atcaggaact gttgcatgat tgaataagac ataattcaaa
     4681 tattcttgat cttttgtagc tttcgttcgg tggttgatca agcgaacaaa actttctgtc
     4741 gccatcgtgc ccattccgcc taaaatactg aaaaaattct ccatgaacta gccttctttc
     4801 acatgaaaat actttttgaa tcttggaata tagttatgga acatatattt catcaacaac
     4861 cagcgcttga ttgatcggtc tttttcataa aatacagtag taccgtaacg attttctttg
     4921 atcatttgct cagctagttt tttgtcttca ttttcccgag catattctaa aaagattttt
     4981 ttgggcacac ccatccataa tcttgcttta tctgattgat tcgttccata ggtcgtttca
     5041 acaaagggtt tgttgaacac tcgatcttct gtgacaaaac gagccagatt caagccgttc
     5101 aatgtcacaa agaaactgct acgaccttgt ctcaaattga tttcaaataa tttatattct
     5161 ccatctctag gatcatactt catatcgaaa ttggcaaagc ctgtatattc gattttttcc
     5221 aaaaatgcct taatggtttg atagattttt tcgttataat ctggcatgat cacaacgtaa
     5281 ttaccaatcg atgctggtgt aggatcttct aatagtggat gaccaaggca catcatccga
     5341 acttgatgat cttcatctac atacgcattt agcacacgca tattactatc atcacccggg
     5401 ataaaatctt gtacaatcat ttcactttta taaccggctt cgtatatccg gcctaaaatt
     5461 aaatcgaact cttctctagt ctctaaaata aaagcttttt ttcttccttc aaattggaca
     5521 gaaagatact ccacactgtt tgcaggtttc aaagcaactg ggaattcaaa aggcaattcc
     5581 tgttccaaat gtccatttac taacatttct tctctgacga tcaatgtttt aggatacgga
     5641 agatcatatt cttcacaaac ttcataaaag cttactttat tgatcagcct ttcaaataaa
     5701 gaataatcaa tatatggaca gataaaatat tctgacagct cttgtttatg ttgggagatc
     5761 aattctgcat atccatcacc acaagcaatc aaaagatatt ttttactctt atcagaatat
     5821 ctttcttttg ccaaacgaag catcgtctcg ataaatacag ggtctttatc aaatcctgga
     5881 ataacttcta cattgacaat tttactgtag cgggtagggg ctaactgatc cgaagcatat
     5941 gcttggcaga ttttgccgta tgcttcatta aaagaacgtg ccataccata aacgttcatg
     6001 tcgcttccta ataatatagg gataaattct tcattttcaa tactgttcat cattatctct
     6061 cacttactca taatctcttg tttttctttt ccaactacac aaggaatatc ataccataaa
     6121 gaaaaaagca atcaaaggaa agtcctccga ttgctcaatt cttttttgaa cggattgaac
     6181 ttataggaag tttttatcta taccaatcag tgtagaaaga actaccatgt aatgattcta
     6241 gaacactgat attaataaaa caagcaaaaa ttcatttttt tatgtttttt taactatctc
     6301 agctatctat cctctttata ccattatttt tttcaaatag tgacctgtat aactttcttt
     6361 tacgttaatg atttcttctg gtgttccagt cgcaaggatc gttcctccac cttctccacc
     6421 ttctggtcct aaatcaatga tataatctgc tgttttgatt acatctagat tgtgttcaat
     6481 cactaaaact gtgttaccag catctactaa tctttgtaat acatgcaaca agcgggcgat
     6541 gtcatcgcta tgaagtcctg tcgttggttc atctagtata tagaaattct ttccattaga
     6601 gattttgtgc aattcactgg caagtttcat ccgttgtgcc tcaccaccgg acaatgtcgt
     6661 tgctggttgc cccatcgtca cataacctaa gccaacatca acaatcgttt gcagtttgcg
     6721 atgaatcttt ggaatgtgct tgaagaattc tacagcatcc tctactgtca tttccaaaat
     6781 atcagcaatg ctttttcctt tgtaatgcac ttctaatgtt tcagaattat atcgtttacc
     6841 atgacatact tcacaaggaa catagacatc aggcaaaaag tgcatttcga tcttaataat
     6901 tccatccccg cgacaagctt cacaacgacc gccttttacg ttgaaactaa aacgcccttt
     6961 cttataaccc cgcatttttg cctcgttcgt ttgagcaaat aacccacgga tatcatcaaa
     7021 tacacttgta taagtcgctg gattacttct cggcgtccgg ccgattgggc tttgatcgat
     7081 atcgatgatc ttttcgatac tttcgtagcc ggaaattgtc ttgaatttac ctggcttagc
     7141 agaattctta tttaattttt gcgctaacga tttctttaag atactattga ccaatgtact
     7201 ctttccagaa ccagacacac ctgtgactgc tacaaattct cctaatggaa attcaacact
     7261 aatatttttc agattgtttt ctgaagcacc agtgatcttg attgctttcc cattaccttt
     7321 acggcgttct tttggtacag ggattgattt tttccctgaa agatatttcc ctgtcaaaga
     7381 attttcatca ttagctactt cttcaggagt gcctgcagcc acgatttgtc cgccttgttc
     7441 tccggcccca ggtccgacgt caatcaaata atctgctgcc cacatcgtat cttcatcatg
     7501 ttcgactaca acgagggtgt tgccaagatc acgcattttc tttaatgagc caatcagtcg
     7561 atcgttgtcg cgttgatgca atccgatcga tggttcatct aaaatataca aaactccaga
     7621 aagattcgat ccaatttgtg tagccaatct gattcgctgc gcttctcccc cagataatgt
     7681 tccggaagcg cgactcaaag ttagatagtc tagtcccaca ttttccaaga atgaaagtct
     7741 gtcatttact tcttttaaaa tcggtttggc aattactttc tcttgttcag ataacgttaa
     7801 gtttgagaag aaatctactg ctttttgaat agacaactca tttgtttgcc cgatattcgt
     7861 tccattgatt tgaacagata gagcttgggg attcaatcga taacctttac atgtctgaca
     7921 agttaattct gtcatataaa gacgcatttg ctcacgcgtg aaatcactgt tcgtatcatg
     7981 ataacgacgc tcgatattct tcaatacccc ttcgaaagtc gtttctacat cgcgaacgcc
     8041 accaaaatca ttttcataat ggaaatggaa aagatcatct gtcccattca agatgatctc
     8101 ctgatgctct tccggtaatt cttcgaatgg tgtatctaaa tcgataccga aactttcaca
     8161 agcctgcgct aacatttgag gataatactg agagctgatt ggattccatg gaacgatagc
     8221 accttgacta agtgtcttcg ttctatctgg aatcactaaa tcttgatcga cttctaattt
     8281 gacacctaaa ccatcacagt ctggacaagc tccaaaaggc gcattgaatg agaacagtct
     8341 tggttctagt tcgccaacag taaatccgca ataaggacag gcatagtgtt cgctaaacaa
     8401 catttcttct tcaccgatca cgtcaaccag agcatagccg tctgctaatc gcagggccgc
     8461 ctctaaagaa tcaaataggc gagaacgaat cccttctttc acaatgatcc ggtcgatgac
     8521 gatcgcgata tcatgttttt tgtttttctc cagctcaggc acttcactta aatcatagat
     8581 ctcaccatcg actcgaacgc ggacgtatcc ctctttttgg attcgttcga atattttttt
     8641 atgttgtcct tttttcttta ccacgatcgg tgccaacact tggatctttg tgcgctcagg
     8701 caaagccatt acttgatctg ccatttgatc tactgattga ctggtaatct cgatatgatc
     8761 gttgggacaa atcggatgtc ctacacgtgc aaacaacaag cgtaaatagt cattgatttc
     8821 cgtcacagta cctactgtcg accgaggatt tttacttgtt gttttctgat caatagaaat
     8881 cgccggactc aatccgtcga tgctatctac gtccggctta tccatttgtc ctaaaaactg
     8941 acgagcataa gcagaaaggc tctctacgta tctccgctgc ccttcagcgt acagcgtatc
     9001 aaatgctagg gaactttttc cagaaccgga caaacctgta acgaccacga atttatctcg
     9061 tggaatcgtt acatctatat ttttcaagtt atgggcacgt gcaccatgaa tcgtaatttt
     9121 atcgttagcc atgttgtcct cctactttga agcttttaat tctaaaatca tatctcgcac
     9181 attcgcggct gtttcaaaat ccaaagcttt tgccgcatct ttcatttctt tttctaattt
     9241 catcaataac gtgtcttttt cttccttcgt catttcttcg tacgagactt gtaccgcttc
     9301 ttttgtatca tcttccgatt ctttcgtaat agaaatcaaa tctcggattt ctttaatgat
     9361 cgtttttgga acgatcccat gttcttcatt gtacttctgc tgaatcgtac gacgtcttga
     9421 cgtttcatcc atcgctagac gcatagaatc tgttactttg tctgcgtaca tgattacttt
     9481 accttcttca ttccgagcag ctcgtccaat cgtttggact aaggaacgtt cactacgtaa
     9541 aaagccttct ttatccgcat ccaagatagc aacaagggaa acttcaggta cgtctaatcc
     9601 ttcacgaagc aaattgattc caactaacac atcaaattca cctaatcgaa gatctcgaat
     9661 gatttctgtt cgttccagcg ttttgatatc actatgcaaa tatttgactt tgatccctaa
     9721 ttctttgaaa taatctgtga ggtcttctgc catttttttc gtcaatgtcg ttacaaacac
     9781 ccgctggtct ttttcgactc gttcatttat ttcgcctact agatcatcga tttgtcccat
     9841 gattggacga acttcgatca ctgggtcaag cagccctgtc ggacggatga tttgttggat
     9901 aacggtatct gtttgttcat gttcatatgg tcctggcgtc gcagagacat aaatgatctg
     9961 attgacatgt ttttcaaact cttctaaccg aagtggccga ttgtctaaag cagatggcaa
    10021 acggaaaccg taatctacta gcatctgttt tctcgctcgg tcaccattat acattcctct
    10081 gatttgaggc atggttacgt gcgattcgtc cgccacgatc aaaaaatctt caggaaagaa
    10141 atccaatagc gtataaggcg gttcaccttc tttacgtccg tccatatgcc gagaataatt
    10201 ttcaattccc gacgtgtagc ccatttctaa catcatttca atatcgtaat tggttcgctg
    10261 ttctaaacgt tgagcttcca gaagtttgtt ttcatttcgc aagacagtta aacgctgttc
    10321 taattccgct ttgatattcg ctacggcatg ctccatatgt tcatcattcg tcacaaagtg
    10381 agtcgccggg aagattgcta cgtgttccgt ttctcctaaa acttcacctg tcagtgcatt
    10441 gacttctcgg atacgttcga cctcatctcc aaaaaactcc acacgtaagg cacgttcatc
    10501 tcttgaagcc ggaaaaatct ccacgacatc accgcgcact cgaaaacgac cgcgctgaaa
    10561 atctatatca tttctttcga attggatact gactaagtca cgaatcaact gattgcgatc
    10621 tagttctgct ccctgtcgaa tcgaaactac ttgtttttgg tactcaaaag gcgagcctaa
    10681 tccaaaaata catgaaacgg atgcaatgac gatcacatca tttctttcta ataatgaact
    10741 agtagccgaa tgtcgtaatt tgtcgatctc atcatttaca cttgaatctt tttcgatata
    10801 cgtatcgctt gaaggcacat aagcttcagg ctgataataa tcataataag aaacaaaata
    10861 ttcaacagca ttgttaggga aaaattcctt gaattccccg tacagctgac cagccaatgt
    10921 tttgttatgg gcaataatca gtgttggttt attcactttc tcaataagat tggaaatcgt
    10981 atatgttttc cctgttccag tcgcacctaa taagatttgt gcttttttcc cgccgactac
    11041 gccatccact aattggttaa tcgcttcagg ctgatcacca gccggttggt atttcgagac
    11101 aagctcaaag tgtcgggagg tatctctctc aatcattctt cgtcctcctt ttcattcatt
    11161 ccttttcatt ttacgaccaa taaaaagaaa aggaaagcaa aggcattcct tactaaaaaa
    11221 ttataccaca ccgaacatac tttcgccact tttttatctt attatacatg ttatgtttta
    11281 taacataaaa tgttagaaac tcgcattttt ttcatttttt cgagcttaca gtcttgtctg
    11341 attcagcact tttcgcctat aataataaaa tattggaaat ttaggaggtt gattatgaaa
    11401 agaaagcatt tatttcactc atttgttttc ttaatgacag gacttgtgac acttgcgttt
    11461 ggtcatgttg cacaagctga caacactaca acggcagagt caagcacaag cgagacaatg
    11521 caaaaaatag agcccaaaaa agacgtatac gtaattgcca gtgactctgc ttttgctcca
    11581 tttgaatttc aaaacgcaaa gggcgagtac gaaggcatcg atgtaaaatt gatggaaaac
    11641 atcgccaaac tgcaaggatt taacatccga atggactacc gtggtttctc tgctgctttg
    11701 caagctttgg aatcaggaca agcagacgga atgattgccg gaatgactgt aacagatgaa
    11761 cgaaagaaaa gctatgattt ttctgattct tactttgata gcgggatcca attagctgtc
    11821 aaaaaaggag atacttcgat caagtcttat gaagatttga aaggaaaaac agtcggagca
    11881 aaagttggta ccgaaagtgc tgaattttta agcgaccatg aatctgaata tggctattct
    11941 atcaaatatt ttgatgctgc tgatcaattg tacgacgggt taaaagttgg cgcaatcgat
    12001 gcaatgatgg atgattatcc ggtaatcggt tatgcgatca aacaaggaca agaattggaa
    12061 acaccgatca aaaaagaatc tggcggaaaa tatgcatttg ccgttaaaaa aggacaaaat
    12121 cctgagcttc ttaatatgtt caatgaagga ttgaaagaat taaaaagaac aggcgaatac
    12181 gataagatta ttaaagaata tgtagcggat ggttcagaaa cttccagtga gtcttccact
    12241 gctgatgaat caacatttct tggacttatc caaaacaact atcaagcatt gttgaaagga
    12301 ctttggagaa caattctcct aactttgatc tcctttgctt tggctatagt aatcggtgtg
    12361 atttttggat tattcagtgt agcaccaata cgcgctcttc gcacaattgc tagtatttat
    12421 gtcgatatca tccgaggaat tccaatgatg gttcttgcgt tctttatttt ctttgggttg
    12481 cctggtatcg ttggatttac gattcctgac ttccttgctg gtgttatcac cttgactctt
    12541 aacgcaagtg cttatatcgc agagattgtt cgaggcggga tcaacgccgt tcctgtcgga
    12601 caaatggaag cttccagaag tttaggtctt tcttataatc ggacaatgca aaaaatcatt
    12661 ttgccgcaag cgatacgcat catgatccca tcattcgtca atcagttcgt tatctcatta
    12721 aaagacacaa cgatcatttc cgcaatcggt gtagtcgaat tgctgcaaac tggtaagatc
    12781 attgttgcac gtacaacaca aagtacgtat gtttacttga tcattgcgat catgtacttg
    12841 attttgatca cagcattgac aaaattagca aaagttttag aaaagaaggt gaagtaagat
    12901 ggcagaaaaa atcttagttg agcatttagt caaaaaatac ggcgataaca cagtattaaa
    12961 cgacatcaat gtatccatca acgaaggaga tgtcgtctgt atcattggcc cttcaggttc
    13021 aggaaaaagt acattcttgc gttgcttgaa tcgtttagaa gaaccttctg acggtgaaat
    13081 tattattgac ggcgcccact tgatggataa aagcacagat atcaacttgg tgagacaaca
    13141 tatcggaatg gtattccaac actttaacct attccctcat ctttctattt tggaaaacat
    13201 cattttagca ccgatggacc tgaagaaaga atctagagaa gaagcagaga aaaaagcaat
    13261 caaattactg cagacagttg gtctagaaga taaaaaagat atgtatcctg aaatgctttc
    13321 tggtggacaa aaacagcgtg tggctattgc tcgtgctcta gcgatgaacc ctgacatcat
    13381 gctttttgat gagcctactt ctgcactcga tcctgaaatg gtcggtgatg tcttgaacgt
    13441 catgaaaaag ttagcagaac aaggtatgac gatggttatc gttacgcacg aaatggggtt
    13501 cgctaaagaa gtggccaatc gcgtcatgtt cattgacgac ggaaactttt tagaagatgg
    13561 aacgcctcaa caaatc
//
LOCUS       DACELF010000072        13211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.075, whole genome shotgun sequence.
ACCESSION   DACELF010000072 DACELF010000000
VERSION     DACELF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.075"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW687_002059"
     CDS             147..1016
                     /locus_tag="IW687_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0951314.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW687_002060"
     CDS             1225..2250
                     /locus_tag="IW687_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0951315.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW687_002061"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW687_002061"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0951316.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IW687_002062"
     CDS             3333..5036
                     /locus_tag="IW687_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951317.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW687_002063"
     CDS             5038..7071
                     /locus_tag="IW687_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0951318.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW687_002064"
     CDS             7082..7522
                     /locus_tag="IW687_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0951319.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW687_002065"
     CDS             complement(7722..8849)
                     /locus_tag="IW687_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951320.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW687_002066"
     CDS             complement(8852..9529)
                     /locus_tag="IW687_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951321.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW687_002067"
     CDS             9793..11559
                     /locus_tag="IW687_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0951322.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW687_002068"
     CDS             11574..12011
                     /locus_tag="IW687_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0951323.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW687_002069"
     CDS             12024..13175
                     /locus_tag="IW687_002069"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0951324.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt t
//
LOCUS       DACELF010000073        13191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.076, whole genome shotgun sequence.
ACCESSION   DACELF010000073 DACELF010000000
VERSION     DACELF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.076"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(148..450)
                     /locus_tag="IW687_002070"
     CDS             complement(148..450)
                     /locus_tag="IW687_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951325.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="IW687_002071"
     tmRNA           complement(670..1036)
                     /gene="ssrA"
                     /locus_tag="IW687_002071"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1207..1980)
                     /locus_tag="IW687_002072"
     CDS             complement(1207..1980)
                     /locus_tag="IW687_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0951326.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2124..2474
                     /locus_tag="IW687_002073"
     CDS             2124..2474
                     /locus_tag="IW687_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0951327.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2455..3171
                     /locus_tag="IW687_002074"
     CDS             2455..3171
                     /locus_tag="IW687_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0951328.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3171..4619
                     /locus_tag="IW687_002075"
     CDS             3171..4619
                     /locus_tag="IW687_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0951329.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4689..5198)
                     /locus_tag="IW687_002076"
     CDS             complement(4689..5198)
                     /locus_tag="IW687_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951330.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5188..5913)
                     /locus_tag="IW687_002077"
     CDS             complement(5188..5913)
                     /locus_tag="IW687_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951331.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6136..8256)
                     /locus_tag="IW687_002078"
     CDS             complement(6136..8256)
                     /locus_tag="IW687_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0951332.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8486..9664
                     /locus_tag="IW687_002079"
     CDS             8486..9664
                     /locus_tag="IW687_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0951333.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9680..10438
                     /gene="aroD"
                     /locus_tag="IW687_002080"
     CDS             9680..10438
                     /gene="aroD"
                     /locus_tag="IW687_002080"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0951334.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10461..10946
                     /gene="ybaK"
                     /locus_tag="IW687_002081"
     CDS             10461..10946
                     /gene="ybaK"
                     /locus_tag="IW687_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0951335.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11017..12270)
                     /locus_tag="IW687_002082"
     CDS             complement(11017..12270)
                     /locus_tag="IW687_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0951336.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12387..>13191
                     /locus_tag="IW687_002083"
     CDS             12387..>13191
                     /locus_tag="IW687_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0951337.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGY"
BASE COUNT         4319 a         2339 c         2628 g         3905 t
ORIGIN      
        1 agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc atcatattac
       61 acaatcttaa tactaactta aaaaatatcc cttttataag tatggtgata aaatccgttc
      121 cgggctacct tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt
      181 atacttcaaa tagtcataac gccgatgatc gctccgtgcg tctggcacat cagtcacgat
      241 atcaaacaaa aaatacacat ctttcttcat tctggttttt gttgccggaa ttttaaagta
      301 gttcttatta gagtaataga gattgattaa taagctatct tcgattgcta aaaagaaaac
      361 ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa
      421 ccataattgt gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact
      481 aatgttcttt ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa
      541 aagtgttctt tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg
      601 aaaataaaaa atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct
      661 gaaatctcac aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa
      721 aatctacgtt catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc
      781 attctgtatc gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta
      841 tcccactaaa gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta
      901 actgttttta ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact
      961 gtaacgtttt tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg
     1021 aatccgtaac gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta
     1081 tagcatatat tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata
     1141 actgcccctt tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa
     1201 aagatcttaa atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt
     1261 ctttgtgata aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt
     1321 gttgtcgcaa gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg
     1381 aactttttcc agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc
     1441 tttcttcact gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg
     1501 ttcgtgatag tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc
     1561 tttaaagcct aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat
     1621 cattgtttgg ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg
     1681 accgttcaac tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga
     1741 atagatttct tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat
     1801 gcccgcatct tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg
     1861 gagaacattg ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga
     1921 tttcgcatct agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac
     1981 aaataaaact ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa
     2041 acctttcgtg aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa
     2101 gaagagaatt taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa
     2161 ttggcagtct ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt
     2221 cgcctcttgc cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact
     2281 ggttttttct tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc
     2341 atacgaatta ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta
     2401 ttatgacaga aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat
     2461 attttaatga ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat
     2521 gaaggttggg aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa
     2581 aacccacaac attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg
     2641 ctagtaggaa gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct
     2701 agagattctg aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca
     2761 aaacctttca gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag
     2821 ttggcagaag caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt
     2881 catttcaaga tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga
     2941 ctgacgccta aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc
     3001 tctcgggagc agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca
     3061 gtggacgccc atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc
     3121 caaactgttt ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt
     3181 tgtatcagca actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag
     3241 tttcttttac acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg
     3301 gatatgctga atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta
     3361 gtcaagatca ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa
     3421 atgtgaactt tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt
     3481 tactgaattt taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa
     3541 aattcacatc gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc
     3601 catttaattt gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata
     3661 tcgataacag cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta
     3721 tcgttgcagt tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt
     3781 taaggaatgc gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg
     3841 acaaagatga atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag
     3901 aatctcaagc agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt
     3961 ctcacgagat gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca
     4021 atgcaatccc tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc
     4081 gtctgatccg tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca
     4141 gtatggtcat taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag
     4201 aagctaaagc agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg
     4261 tttacgctga ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac
     4321 agtttactga aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca
     4381 tcacgattga agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc
     4441 gttattataa agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt
     4501 taccaatcgt gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac
     4561 taggaaaagg aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa
     4621 aaacaagcgg tataaaagat catgaatcga tcttctatac cgcttgtttt tactataaaa
     4681 tgattgattt agcccgcggg ctctaaacga tcaatataag gactttgagc aatagtagca
     4741 tcatgagtaa agagtaagat accttgattt tcttcatgag ctaaataaga aagcaagtgg
     4801 agtagctctt ggatctccaa tacactaagt tcatcaaatg tatcttcaat aacaatattg
     4861 tttcgcttgg aaagcaaggc atgaagtaat tgcagcttta ctttttcaaa agacgtcaac
     4921 tcagctgccg cacaattaag caaggaagga gagatatcaa gtttttcttg ataggccaaa
     4981 cgatcttgtt tgatttcttt ttccggtaca tccaacagga catggtcttt aagattcaga
     5041 taaggaaaca tcggccaatt tttttggatg atcgcaaaat tatccagttt tgcaacaaga
     5101 acctcttttt tcaagtgctc caatatttta tgctgttttt cgagaggaca caaaataacg
     5161 gtcaagtgtt cagcatgaat caaatattca ggtttcatta cgtccaggcc tcctactttg
     5221 caaatgatgg ataaaaaaag tgaaagggaa tagactaaga atgagacagc atccactgag
     5281 cccaataaga gtttgggcaa atcccttaag tatatctgtg aaaaaaagat cattttggaa
     5341 atcgatggta aaaaaagtct gttgatccat cggaattgta agtccgctta ccgaagtcaa
     5401 gatatcacgg atatctggca gttcagtagt ctttaagaca agaaaattga tcctttgaag
     5461 aagcgcttca taaactgact ggaagcttac gatccacaat aaaaccaaga tacaacaaag
     5521 aaagagagga aagaacagct gccagaaaat aaaaaggaat aatttggttt tagaaagacc
     5581 agtcaggtgc caattgatca attcctgctt gttttgcctg actgaatagt aaaagaaaaa
     5641 gataaaaagg aaggcaaata gaaaaaataa actgaatatg ccttttatat aaaatcggct
     5701 agctaattct aaagccggaa gaagctctgt agatgcagaa gcgccaataa atgaaagccg
     5761 atcttggact tgattggcta aagccgattg caaatggtaa agagtcaggc atgctatcaa
     5821 taaaaaagca gaaaagcttg aaaataaaca atagagtatt gtctgctttt tgtaataaat
     5881 taatcgagca gtagcatagc ggattatttt catacttttc acctctataa aaagtataga
     5941 aaatgaatat gaataaagta tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa
     6001 tattttgtaa aaagcaagag gttgtgaaga agctgcccta ttctattacc gtagatgcaa
     6061 gcttttgaac actgtttatt cgaaaagagg agcagcgaca ataattttgt cgctgctcct
     6121 tttaaattta ttttatcatt cggaagaact tgtggtcgtt ccgtgaagtt cttggtacgt
     6181 ttgcgtttta taaagatcaa cagttgattg tccgttgtgt tcactaaaga cactagttga
     6241 cttgtcacca agagattttt cagcttcttt taatttctct aagccattct tgtagtcata
     6301 atcactggta tcaattgggt ctaatccact attcgtatag aaccgaagaa gatcaccatt
     6361 attgatttgg tcagaaacag ctaactgttt atcaactttt tctttcagtg aatcaatttc
     6421 attctgtact tcctcagtag gttcagtaat agcttcacca gtttcgttac tatagatcgt
     6481 accaccgtag taagtatatt ttggtgtaac aaaatcacca tcacggaaag aaacaagctg
     6541 ttcatgatct ttcgagaata gatcttgtcc taactgaata taattttttg tatctacacc
     6601 tagtaagtga agtaatgtag gcaaggcatc aacttgtccg ccgtaagtgt gattgattcc
     6661 gccctgtgtt tgtccaggga tatggatcat ataagggaca cgctgcattt gtgcattgtc
     6721 gtagtttgtc caagtagaac ttgtttttcc aactaattca gctagatttt tatttcttga
     6781 attcgagaca ccgtaatggt caccgtaaag gacgatgact gaattatcat acaagccgct
     6841 ttctttcaaa taattgaaga attcttgtac ggcggtatct aaataattgg ctgtcgcaaa
     6901 gtaaccgttg atcgtttcat catttgtatt ggctaacggg aatcctgctt cgtcattggt
     6961 gaattttgaa taaggataat ggttagatac agcaatgaat tttgaataaa atggttgttg
     7021 cagacgttcc aagtattgta cggattgttg gaaaaatggt ttatcatgca acccgtattg
     7081 gaacgaattg tcatcattaa catcataata agaagaatca aagaaataat catatcctaa
     7141 attcttgtat gtctcgtttc gattccagaa tgtaccagcg ttcccatgga ataccgcact
     7201 aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct tggaaagtat tttttccgcc
     7261 taattgtgtg aacaaggagc cttgattcaa gccaaacaat gaattttcca tcagcgtttc
     7321 cgcatcgctt gtttttcctg cttttacttg atggaaaaag ttgtcaaaac tgaacgtgct
     7381 gttgctgtgg taaagactat tgatgaaagg tgtaacttca tgctggagac cattctcatc
     7441 tgtcaattta taatcgatca ggaattgctg gaagctttct aaatggatat aaatcacatt
     7501 ttttcctcta gcgagcccga acaaatcgtc atttggtgct gcataatgtt cttttacata
     7561 attttctact tctttcatat cattgggact tgcttctgcc cgcacttggt ttgtctggta
     7621 agtttgaaca ccgtcataaa ccatgaaagc attcaagcca agatatttga ccaaataatc
     7681 acgagagaat gttcgagtca gcaattctgg acgatcagct tccgccataa aaagatttcc
     7741 tgagaaaatc ataactgcta atgtagagat cgcaaaagcc attcttgctc ggatcgggcg
     7801 agggtccaac ttgatttttt ttgcaatcag cagaacagtc aagataataa aatcgatcca
     7861 atagatcaca tcatatgggc gaaacagtcg aatggcactt tctcccagac cactcgctac
     7921 tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc gtgaattctc gataataagt
     7981 gacattcgaa aaaacaagca atgtcaaaag aaaataaatg accatcatcg tgatataagc
     8041 aactctaggg cgtctgatat aaagagcaat tccaagtaat aacaatgtag ttgcaatagg
     8101 gttgataaac aagataaagt attgaatgcc actttcgatt ccgagatgga aatcaactaa
     8161 ataggcaaaa atcgttttca accaaagtaa aacgactagt aaagaaaaaa atccgagcct
     8221 cttattcaaa aaggcaggtg tttttataca tttcaagata atcttccttt ctgcacttct
     8281 taatcacaga agtgtcctga aaccatgtta cacagctcac ttattctcat ttattttact
     8341 agtatgacaa tatagtgtca attgaaacca gaagtttatg ctgtttcatt aaggttttct
     8401 ttaagcttca tgaaaaaatg agcagtaaga caagtcctat gttttttgtt atacttggga
     8461 agagaaagct aaagcaggtg aaggaatgaa agtaaaagta aaaaaaagag caagcaatcg
     8521 attgaaaaaa ggatatccac tgatccaaaa agaagacctt gctcatcccc aaaaagaaac
     8581 cacgcaatgg gtggaattag ttgattcgca aaaccaattt atcgcaaaag gatatctagg
     8641 aaagcagaat aagggaatcg gttgggtatt gagccagcag aacatcccat ttgatcaatc
     8701 tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga actttctttt tttcagatga
     8761 ttctactacc gcttttcgta tattcaatgg agaaggagac gggatcggcg gtctgattgt
     8821 tgattattat gcaggttatg ctgttttttc ttggtataac gaaacgttat atgcacatcg
     8881 tgaattattg attcaagctt ttcgtgaaat ctacccggaa atcataggag gctatgagaa
     8941 aattcgattt gaatcagtaa atctgcctga gagccagttc atttacggaa aagaggcacc
     9001 ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc gtttatttaa atgaaggatt
     9061 aatgactggg atctttttag accaaaaaga agtacgggga tcattagcag aaggtttcgc
     9121 ttctgggaaa acagttttga acatgtttag ttataccggc gccttttctg ttgctgccgc
     9181 aatgggaggg gcaatcgaaa caacaagtgt cgatctagca aaacgcagtt taaaaaaaac
     9241 acaggaacag tttgaagtga atggtttgga tgtcgatcag caaaaaatca ttgttatgga
     9301 cgtattcgag tatttcaaat acgccaaaag aaaacaattt ttatatgaca tgattgtatt
     9361 ggatccacca agttttgcca gaaataagaa aaaagtcttt cgcgtagcaa aagattatgg
     9421 ggaactggtc aaggatagtc tggatatttt agcagaagac gggatactta ttgcgtctac
     9481 aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg attgaagagg aattccatgc
     9541 caaaaatgtt tcttttgaac aacttgcttt atatcgtctg ccaaaagatt tccagacgac
     9601 ggataccttt ttagaaggca attatctgaa agtgtttgtt tatcaaatta aaaataacca
     9661 ttagaaggag cgatgcagaa tggttcgggt aaagggattg aatataggaa gcggtagacc
     9721 aaagatttgt gcgcctctta tcggggccgt ccgagaggaa gtattacgtg aagcagttct
     9781 tgcacgtgaa gcaggggccg atttagtcga atggcgagtc gatcattatc gtgaagtttt
     9841 ttcttttgat gaagtagcag aaacattggt tcttatccat gagatgcttg atggactgcc
     9901 gttattattt acgtttcgta ccttagctga aggcggagaa caagacatat cgatccgtca
     9961 gtatcgcgac ctttatgaac atgtgataca cacaggatta gtagatatgg tcgatatcga
    10021 gttgttcaaa gtagaaagtc taggaaaaag actgatagag gaaatcaagt ctttaaacat
    10081 tcctttaatt atcagtagtc atgattttaa agaaacacca gcagaccctg tcttgcttta
    10141 ccgattgaat gttatggaac attttggagc cgatatcgga aaactggcag taactcccaa
    10201 taatgaacga gatgttttgc ggttgatgga gttgactaga cgggcaaatg cttttgtctc
    10261 catgcctatt attacgatgt ccatgggaaa tcttggtaaa atttctcgtc tggttggcgg
    10321 aacgacgggt tccgttatga catttggttc cttaagcagc gattctactt ctgctccagg
    10381 acaattaccg gtcaaagcat tgaagcaaat tattcaatta ttaaatgaac cgttgtagaa
    10441 caagtttgag gtgatatttt ttggcaaaga aaaaaatagt aaaaacgaat gcaatccgta
    10501 tggtcgaaca aaaaaagatt ccctatacag agcacgaata tgagtgggac gaaagccacc
    10561 taagtgcgtc atcggtcgct gaacagcttc cagaaagcca atctcgtata tttaaaacac
    10621 tggttgctgt gggcaatgtg acggggcctc ttgttgctgt gataccggga gaagccgaat
    10681 tgaatctaaa aaaattagca aaagtttcag ggaataaaaa agtagaaatg ctccatttaa
    10741 aagatttgga agcaacgacc ggatacatac gtggtggctg ctcgcctata ggtatgaaaa
    10801 aactatttcc tacttattta gatcaaattg cagaaagcta tgaacaaatc attgtctcgg
    10861 ctggcagaag agggctacag atggagttag cacctcaaga tatctgtgca ttaacgagcg
    10921 gacaatttgc agatataaaa caataaatca tacgtataaa agaaagaagg aggccgggat
    10981 gttgtttgtc cagtctcctt ctttcttttt tatacattat gctctttgcc cggcattcat
    11041 tcctgctaca tgccctgtag cgaaagccgc tgtgatatta aagccgccag tataaccatt
    11101 gacatccagt aattctcccg caaaatacag accatcaatg attttactct ccaatgtttt
    11161 aggataaatt tcctttagag aaacccctcc gccagttaca aatgatttct caataggaaa
    11221 agttttgttg attgatagct taaactcttt gcatagttga acaaaatctt gaatttgttt
    11281 ttgagaggct tgttgtcctg tcagatgatc catttcaagc cgctcaagaa agaaaactaa
    11341 caatcgctca ggcaatatac catgccaagc attgaccaag ttctttttgg ttgctttact
    11401 tttttctgtc aatatatgaa taagctcttg tttggtttgt gtagggaaac aatccagaga
    11461 aagtgtcact ggttctcccg ttttctctaa ttctttattg acaaaacttg aacaacgcag
    11521 tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa tccatcgtat gttcagtcaa
    11581 tacacgacct tttttattta aaacgcgtaa agtgatgtct tgtaaagaaa gcccttgaag
    11641 tgttttctcc tgaatatacg gttcatccga tatcaagggg gactctgtcg cataaagagg
    11701 cgtcaccgtg tgacccactc gcttcacgat cttgtaccca tctccagtag aaccagtaga
    11761 tggataagtc cgtccaccag ttgttaagat aatggcagga gcatggaact cttcgaattc
    11821 cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat ttctctactc gagtagagaa
    11881 tagaagcgtc acatctagtt ctttcagacg attgagcaat gcttcgataa tcgtttttga
    11941 tttatttgtt acaggaaaca tccgtccatg atcctcttct tttaaatgta cgccttgtga
    12001 ttcaaagaag ttcatgatat cgaaattatt ccattgcgca aacgtactat ataaaaactt
    12061 tccatttcca ggaatatgtg caatcaaatc atcaacagga cgattgttcg taacattgca
    12121 ccgaccacca cctgtcatca gtagtttttt tcctgctttt ttgttttttt cgatcaagag
    12181 tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc atcccgcttg ttccagcgcc
    12241 gactacgatc acatcataca gtttttccat tccctcacct caaggaaagt gtagcatatc
    12301 tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga gaaaatgagc taaaatgagg
    12361 gaagataagc aaaggagcgt ggaacgatga caaacgcaac agaatatgtt caagcaattc
    12421 aagaaaagct acatcaaaag gatcaagggc aagaagaatt tttacaagca atcgatgaat
    12481 tcatgcccac ggttatgtct tttttggaca ctcatcccga atatatcgaa aagaatatct
    12541 tagaactttt aacggaacca gaaagagtta ttcaatttcg agtgccttgg caagatgatg
    12601 aaggaaactg gcgagtaaat agagggtacc gtatccaata caactcagct atcggccctt
    12661 ataaaggtgg attgcgcttc catcctagtg tcaatctaag tatcttgaaa tttttggctt
    12721 ttgaacagat tttcaagaat agtttaaccg gtctgccaat cggtggagga aaaggcggaa
    12781 gtgacttcga tcctaaagga aaatctgata atgagatcat gcgtttttgt caaagtttca
    12841 tgctggaatt gtcaaaacat atcggcccat cgattgatgt tcctgcggga gatatcggtg
    12901 taggagctag agaaatcggc tatttgttcg gcgaatataa acgattgaaa aaatatgata
    12961 ctggtgtgtt gacaggtaaa cctttggatt tttggggaag taagatacgt acggaagcta
    13021 ctggttatgg attagtgtat tatgtcaaac atctattgaa cgaagaaaaa gattcattta
    13081 atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc catttatgca atcgaaaaag
    13141 tgcaagagct aggcgggaaa gttgttacat gttcagattc tagcgggtac a
//
LOCUS       DACELF010000074        13083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.077, whole genome shotgun sequence.
ACCESSION   DACELF010000074 DACELF010000000
VERSION     DACELF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.077"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW687_002084"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW687_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0951338.1"
                     /translation="MVSSIITALYLLTALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IW687_002085"
     CDS             1873..4539
                     /locus_tag="IW687_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0951339.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IW687_002086"
     CDS             4903..5655
                     /locus_tag="IW687_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0951340.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IW687_002087"
     CDS             complement(5677..6831)
                     /locus_tag="IW687_002087"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0951341.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IW687_002088"
     CDS             7016..9457
                     /locus_tag="IW687_002088"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0951342.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="IW687_002089"
     CDS             complement(9787..10491)
                     /locus_tag="IW687_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951343.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="IW687_002090"
     CDS             complement(10678..10923)
                     /locus_tag="IW687_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951344.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="IW687_002091"
     CDS             complement(10940..11791)
                     /locus_tag="IW687_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0951345.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="IW687_002092"
     CDS             11957..12961
                     /locus_tag="IW687_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0951346.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4187 a         2319 c         2724 g         3853 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcagttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaaa aaaagagcgt gagacaaaag taaagaacac
    13081 ttt
//
LOCUS       DACELF010000075        13058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.078, whole genome shotgun sequence.
ACCESSION   DACELF010000075 DACELF010000000
VERSION     DACELF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.078"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(211..1167)
                     /locus_tag="IW687_002093"
     CDS             complement(211..1167)
                     /locus_tag="IW687_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0951347.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1271..1873
                     /locus_tag="IW687_002094"
     CDS             1271..1873
                     /locus_tag="IW687_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0951348.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1961..3460)
                     /locus_tag="IW687_002095"
     CDS             complement(1961..3460)
                     /locus_tag="IW687_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0951349.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3476..4000)
                     /locus_tag="IW687_002096"
     CDS             complement(3476..4000)
                     /locus_tag="IW687_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951350.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4298..5047
                     /locus_tag="IW687_002097"
     CDS             4298..5047
                     /locus_tag="IW687_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0951351.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5038..5823
                     /locus_tag="IW687_002098"
     CDS             5038..5823
                     /locus_tag="IW687_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0951352.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5792..6508
                     /locus_tag="IW687_002099"
     CDS             5792..6508
                     /locus_tag="IW687_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0951353.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6610..7629)
                     /locus_tag="IW687_002100"
     CDS             complement(6610..7629)
                     /locus_tag="IW687_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0951354.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7648..8706)
                     /locus_tag="IW687_002101"
     CDS             complement(7648..8706)
                     /locus_tag="IW687_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0951355.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8927..10075)
                     /gene="nagA"
                     /locus_tag="IW687_002102"
     CDS             complement(8927..10075)
                     /gene="nagA"
                     /locus_tag="IW687_002102"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0951356.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10151..10987)
                     /locus_tag="IW687_002103"
     CDS             complement(10151..10987)
                     /locus_tag="IW687_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951357.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11171..12388)
                     /locus_tag="IW687_002104"
     CDS             complement(11171..12388)
                     /locus_tag="IW687_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0951358.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12771..12854
                     /locus_tag="IW687_002105"
     tRNA            12771..12854
                     /locus_tag="IW687_002105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12805..12807,aa:Leu,seq:gag)
BASE COUNT         3895 a         2684 c         2282 g         4197 t
ORIGIN      
        1 accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct accgttcagc
       61 tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa tattgtgact
      121 tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa atcagctttt
      181 tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt gggagaattc
      241 tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc
      301 ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat aaacttcttc
      361 aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg cagtataaat
      421 caataaacca tagatatgtt ttccataata agctggttgc tggattctct ctagattttc
      481 cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag tttcgtagta
      541 tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt ggctttcgcg
      601 aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt ctgataaaag
      661 aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta tttggttttc
      721 cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa atactaatcc
      781 aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt cctccactaa
      841 ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga agcgagaggc
      901 aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc caacagcaaa
      961 aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat tcatgcccgt
     1021 acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc ctgcagctgg
     1081 ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac tgatcactgt
     1141 tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta attatacttg
     1201 aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag
     1261 gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca aaagtgcctt
     1321 agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc cgatgattgc
     1381 tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt cttttggaaa
     1441 gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt tttttgtcaa
     1501 aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc tagttattat
     1561 tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg caatagctac
     1621 gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg ctatccaacg
     1681 tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct ttcttcgtcc
     1741 tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc aaaagaaaga
     1801 acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac aaaagaaaaa
     1861 tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa
     1921 gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc ttcatcgagt
     1981 tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc ccacgtgtcg
     2041 acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag
     2101 tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg gtttctcctg
     2161 aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc tgtgctgctt
     2221 cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct tcgccatatt
     2281 ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct tttgctagtg
     2341 ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct tctgccagtg
     2401 cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt tgagcttgtg
     2461 cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc gaatcatatt
     2521 gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga cgttctacga
     2581 ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt tctaaattgt
     2641 aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt gctaatttca
     2701 gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg
     2761 attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc gcttctgcta
     2821 tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca agataaccgt
     2881 ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt tttgcaaggt
     2941 ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga tagatttctt
     3001 ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct cttgcttcat
     3061 tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca atctcttcta
     3121 cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg ccttgttccg
     3181 tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc cgattcgaac
     3241 gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt ttgttattgc
     3301 tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc aacgcttcat
     3361 ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt aaaatcaaag
     3421 ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg ctcctttaag
     3481 cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc tcaatgatca
     3541 atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa gctggacgac
     3601 taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca gggatcggtg
     3661 tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc gttgattcgg
     3721 catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact atcgatatgc
     3781 ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca cctagaaaac
     3841 caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc gggccatcaa
     3901 aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa atcagcaatg
     3961 tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct ccttccattt
     4021 ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg actcagtggt
     4081 gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg ttttcatgtt
     4141 aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta agaaaaaacc
     4201 attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg tttctttgat
     4261 tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta gtgtgtcaga
     4321 taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa aaaagctgca
     4381 gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga ttgcatcgaa
     4441 tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg cagacccaag
     4501 ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg gaggacaaga
     4561 atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg tccctattta
     4621 ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca cgattgacaa
     4681 tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac ttagcgtagg
     4741 catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc
     4801 acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag tagatgaagg
     4861 aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg caaaaaacat
     4921 tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag tgataatcta
     4981 tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac tcaatccatg
     5041 tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa tgaccgtttt
     5101 ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat ggaggagctt
     5161 ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt catgaataaa
     5221 cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac ccattttatc
     5281 cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct gatcaaagta
     5341 cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca atctgttgaa
     5401 atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc catccattca
     5461 cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct acaaaaacat
     5521 gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga agtattcatc
     5581 gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa aatatttacg
     5641 ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat ccattcaatc
     5701 gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg tacaatcgtc
     5761 tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga taatcgaaga
     5821 tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta atatgaacgt
     5881 aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga
     5941 tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt tatcttggct
     6001 tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg atatcgaaac
     6061 cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt tcactttgca
     6121 acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc aaacgactga
     6181 aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca ttcacgaaat
     6241 accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc
     6301 atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa aaagctcact
     6361 gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata tattgaacga
     6421 aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga caaagaatgg
     6481 gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa aatagtccaa
     6541 gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt gacgaatatc
     6601 catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt tcgtttttcc
     6661 agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg ataatgcctc
     6721 atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt ctactaattg
     6781 ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa aaacctctgt
     6841 ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca aagctcttaa
     6901 aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc tacctttttt
     6961 catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga tatgaggtac
     7021 atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga acgtcaatac
     7081 ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc ctagtccctt
     7141 aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta ctgtgcaatt
     7201 tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag atgttcggat
     7261 cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata actctgctat
     7321 cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag ctgccgcttg
     7381 tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc catattcctg
     7441 catttttgca agattggtct catctgctat acaatttccc ctcaacaata cagcatcatg
     7501 gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac tgagatcata
     7561 atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc
     7621 gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat gaatcatttg
     7681 taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc
     7741 attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg ccactgttgc
     7801 agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga ctggtagaat
     7861 attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa ataccacttt
     7921 gactagactg taataccatg aagttttttc agatgcagct tttttgaagc ttacttcacc
     7981 taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta aataatccat
     8041 cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt ggcttatctc
     8101 tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc aaatgaaaag
     8161 gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat ttttagcttc
     8221 catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc ggtagccaat
     8281 attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga cgataggcgt
     8341 tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt gcttatcccc
     8401 tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa taactaatcc
     8461 ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt gcatgaaagc
     8521 tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa tttttccaac
     8581 agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa taaaaactgt
     8641 tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa
     8701 gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc ttttattttg
     8761 ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga acaataggaa
     8821 attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa aaaaaacctt
     8881 aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac
     8941 gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt
     9001 ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg
     9061 gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat
     9121 ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg
     9181 ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc
     9241 caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta
     9301 ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat
     9361 gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac
     9421 cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta
     9481 gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa
     9541 actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag
     9601 cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg
     9661 cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc
     9721 ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc
     9781 ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca
     9841 tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat
     9901 gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc
     9961 cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg
    10021 catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat
    10081 tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg
    10141 tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga
    10201 atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat
    10261 cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt
    10321 ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt
    10381 gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac
    10441 tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac
    10501 taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta
    10561 atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa
    10621 caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat
    10681 cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca
    10741 acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat
    10801 caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct
    10861 cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt
    10921 gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg
    10981 tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag
    11041 gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa
    11101 aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg
    11161 gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt
    11221 gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat
    11281 taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt
    11341 tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag
    11401 cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc
    11461 tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta
    11521 gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca
    11581 tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc
    11641 ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca
    11701 aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat
    11761 agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta
    11821 ataattcttt tggataaagt gccccagtag gattattagg attaatgata acaatagctt
    11881 ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt
    11941 catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg
    12001 gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca
    12061 tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa
    12121 aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat
    12181 ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg
    12241 cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt
    12301 cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta
    12361 atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag
    12421 aactttccaa attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt
    12481 gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa
    12541 ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt
    12601 cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc
    12661 attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt
    12721 gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt
    12781 tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag
    12841 tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga
    12901 acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc
    12961 atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc
    13021 aagtgttaaa acaattaatc gaaaaacgag taaaatcc
//
LOCUS       DACELF010000076        12954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.079, whole genome shotgun sequence.
ACCESSION   DACELF010000076 DACELF010000000
VERSION     DACELF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.079"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(73..1182)
                     /gene="rpoD"
                     /locus_tag="IW687_002106"
     CDS             complement(73..1182)
                     /gene="rpoD"
                     /locus_tag="IW687_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0951359.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1206..3047)
                     /locus_tag="IW687_002107"
     CDS             complement(1206..3047)
                     /locus_tag="IW687_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0951360.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3229..5622)
                     /locus_tag="IW687_002108"
     CDS             complement(3229..5622)
                     /locus_tag="IW687_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0951361.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5729..6589)
                     /locus_tag="IW687_002109"
     CDS             complement(5729..6589)
                     /locus_tag="IW687_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0951362.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6648..7169)
                     /locus_tag="IW687_002110"
     CDS             complement(6648..7169)
                     /locus_tag="IW687_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0951363.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7489..8208
                     /locus_tag="IW687_002111"
     CDS             7489..8208
                     /locus_tag="IW687_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0951364.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8234..10825
                     /gene="mprF"
                     /locus_tag="IW687_002112"
     CDS             8234..10825
                     /gene="mprF"
                     /locus_tag="IW687_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0951365.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11013..12200)
                     /gene="tuf"
                     /locus_tag="IW687_002113"
     CDS             complement(11013..12200)
                     /gene="tuf"
                     /locus_tag="IW687_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0951366.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12568..12924
                     /locus_tag="IW687_002114"
     CDS             12568..12924
                     /locus_tag="IW687_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0951367.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3715 a         2711 c         2302 g         4226 t
ORIGIN      
        1 gtctcaagct ttttttcttt taaaataaac caaacagttt tactgatttc tcttctggtc
       61 aatagtccac gcttattcta agaaatcttt taattgtttt gaacgagaag gatggcgcaa
      121 ttttcttaat gctttagcct cgatctgacg aatacgttcc cgagtaacac caaatacttt
      181 ccctacttct tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac
      241 attctcttcc cgatcagtca atgtatctag tacatcttcc aattgttctt ttagtaactc
      301 ataagctgcg tgttctgctg gacttgttgc ttcctggtct tctatgaaat cacctaagtg
      361 agagtcatct tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa
      421 gatttctcgg actttttctg ttggaagatc catttccgca ccgatttctt ctggcgtagg
      481 ttctcttcca aggtcttgca gcaattgacg ttggatacgt atcaatttat taattgtttc
      541 aaccatatga actggaatac ggatcgtacg tgcttggtca gcgattgctc gagtgatggc
      601 ttgacgaatc caccatgtag cataggttga aaacttaaat cctttgcggt agtcaaattt
      661 ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga actgcatgcc
      721 tcgtcctaca taacgtttag cgatacttac tactaaacga aggtttgctt ctgccagacg
      781 ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct aacgctactt cttcttccgc
      841 agttaaaagg gagacacggc caatttcttt cagatacata cgaaccgggt cattgatttt
      901 tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt tctcagcact
      961 cttcaagctg tgaattgatg gatcaccatt ttcatccacc acactgatac ctgcgtcttc
     1021 aatcttttgg atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg
     1081 attggataaa tcatcatata caacttgtcg tttgggtttg ttttctttga tgaaagctgc
     1141 tactgctgct tcatacgttt ttttatctgt tccatttgcc ataaaagaag gccccccttg
     1201 agtagctatt tggcttgttt taactgtttc gttaaattga tgatttcaat cgtcaattca
     1261 agctcaagct gtttgtttcc agttcgtctg gcttcttgct gcatttgttt ttttaatgtt
     1321 atttcatcag ctaagccaga tttgctgatc acatctagca agtcttggat ttctcgtggc
     1381 gaactttctt ctgccaccgg taaatattcg atttccacca ccaaccgcct taaattttca
     1441 tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga gacatatgca
     1501 tcgaataaaa aataaacttc tgcatatgca tcatgaataa actgaaattc tcgttctttt
     1561 aaaatattac ggacagaaac ttctttaaat gcacgatata atagaatctg ctctgccttt
     1621 tctacttgcg tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt
     1681 tgactttcct gaggttcttg acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt
     1741 ttttgtatcg tctctctgcc gatctgaaac tctgttgaca gttgagatag atacatatct
     1801 tgttcgattg gtgaaacaac tcggctaagt tcaactagta attcttcgag gtattcgatc
     1861 ttgtcttttt cattatctaa atttttccct tgttttaaat aacgtgattt aaaagtaaag
     1921 actgtttcgc gcccatgagc cactaattct tggaaagatg cttctccata cttacggaga
     1981 tattcatcag gatcaagctt ttctggaacg actacgacag aaagtgctaa tcgactgttt
     2041 tgccgaagca attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag
     2101 caaaagacca attctttcgt cagcttttcc atttgttgga tctgttgagc tgttagactt
     2161 gtccccatag aagctaatcc agttttgata ccagcctgcc atgcagcaat cacatccata
     2221 aacccttcga acaacaaaat ttctccgttt ttccggatat cgcttcttgc tttatcaaga
     2281 ttaaataaaa cttgtctttt attgaacagt tctgtttctg gactattcaa gtatttgggc
     2341 tgatcttttt cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg
     2401 ccttgagaat tacgaagtgg aaacatcact ctttgataaa aacgatcaga catacggcca
     2461 tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt catttgaaat tgattcattc
     2521 ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt gaattcttca
     2581 attaattcta aagacaaccc acgactttgc aaatattcta aagctggctg tccagctttt
     2641 gtatgcaata gcatgtgatg atagatttcc gctgcttttt catgagcagc aatcagtttc
     2701 cccgtagcag attcaggatt ttggacagct atagtacgct gacgccattg atcttctaac
     2761 ggtatctgtt cgaagtctgc cacttttacc actgcttctg gaaaacttag accttctagt
     2821 tcttgaagaa aagtaaacac gtttccgcct ttaccacaac caaagcaatg aaaaatctgc
     2881 ttgtcttctg caacagaaaa agacggtgta cgttcttcat gaaaaggaca cagccccaag
     2941 taatttttac cagacttttt caactggaca tattgtccaa tcacctcaac gatattcgtt
     3001 tgactgcgta ctgtttcgat aacctcttga ggaatccgtt gagccatttt ttcaccccct
     3061 gctctattga tatcttttgg gtttaacaga agatatttca ggagaataac cggtaaaaaa
     3121 tcgcactaac tgaaatcagt gcgattttaa aggacacaat tatacatgtt tgattgtatc
     3181 atatttcatt gctttttcaa gtataacaca tttctatctc attaagcatc aatttgctac
     3241 tcttttcagt aactgatttt gaatatcagc cttccagtac aatactcgaa gaacaatatt
     3301 tacgagcatg aatacaaaga agtatcctac gctctctccc aaccctaagt ttacttccaa
     3361 aattccatgg aacagcattg cagctacata tatccctacg atcgtcgtta ctgctaggaa
     3421 gatgcgcaat gggttgaagg gataacaagc ttttacaact gccatgcaac tgatcccaat
     3481 cagcaagtaa tacatcaaag tagtagtttc agcatttccc catgcgaatg tttttccaag
     3541 tagatacacc gcaatgatat tcactactac aagcaaggca tttggtaaag cattaataag
     3601 tgctgtaggc aaataacggt aagtgatttt tcttttatct tgttcaaaag ataaaaagaa
     3661 ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt tggataggga taaacggaaa
     3721 tccaattgct gtaacggcac atataatcga taaaataaat gaataaatcg ttttgataaa
     3781 aaagatgcct gagactttcg tcacattgtt gaccactcgg cgtccttcaa acaggacttc
     3841 tggcaatgta gtaaaatcag agtccaacaa taccagattc gcaatctgac gtgttgctcc
     3901 atcaccttct gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa catcattcac
     3961 accgtcgcct gtcattgcaa ccgttctgcc gctttctttc aattcattta ccagcaattt
     4021 cttttgctgc ggactcactc gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc
     4081 catttcttca gtaatttgag aaagatcgac atacgcatca taattcggta atcctgcacg
     4141 tctagcgata ttggagacag taaaaggatt gtctccagaa atcactttca gatccacacc
     4201 ttcctcatga agataagcta gtgtttcttg agcgtttttt cggattggat catcgatttc
     4261 aaacagagca accggctcaa caaatgctgg tttttcttcg ttcaattgct gatttggagc
     4321 gatccccaac ataagtacgc gatagccatt cgcttgggct gtttttagac tgtccggaag
     4381 ccgatcttct tccaccaaac gttcaggcgc tcctacgtac acgactccca gttctggaaa
     4441 ttccatcgcg ccccatttgc gatcagaaga aaatgcgact ttgtttgtca catcataccg
     4501 attagaaagc tcaaaataat ctctcatagc ccgcatagtg atgttgttat cactgctttc
     4561 tgccagatac gtccccataa tttctgggat ggctttctcg tacagcggat gtagtgcttc
     4621 tacccgctgg atactcattt tcccttcagt aatggtccct gttttatcca agcataatgt
     4681 atcgacatgg gccagcgttt caattgaata catgtcttga acaaggattc gtttttttgc
     4741 cagtttggtt acccctgtcg ttaatgcaat acttattagc aataccaaac cctttggaag
     4801 catgcctaac aaagctgctg cagatgcaat aacagaaatt ttgatagaag catcgcggat
     4861 gaacagtgct tccaaaaata gtatgatccc caaaggaata atcacgaaac tagtgaattt
     4921 ggatactttg cgaatcgaat ctacaagttc agaacgaata gctttgtgag tctttgcctc
     4981 gctagtcaag cggacagcat aattatctgc acctactcgg ataacttcag ccatgacaga
     5041 accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca gtttctttga caatcaaatc
     5101 agattcgcct gtcaaaagag cttcatttgc ttctgcccgt ccagatataa cctgtaaatc
     5161 ggctggtatc tgttctcctg ctccgatttt taccaaatct cctaatacca gctctttagt
     5221 agaaatcgat tgttctttac cgtcccggat cactagtact tctgattggg aaacaatcga
     5281 taatttccgt acaagatttc ttgcatgtat ttcttgataa attccaataa cgatattgac
     5341 gacaataatt gctaaatacg ccatgttgga atacgcacct acaaataaca gacaaatccc
     5401 aataccaaag ttcagaaaat tgaataacgt caatatatta tcgctgaata tttgttttgt
     5461 actttttgat gcatcttcct ggtagtcatt ttgctggcca gaagccattt tctgaactac
     5521 ctcttgctgt gtaagtccat tcattttctt tttctccatt ttccgccaac tttcaaaaat
     5581 aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac aacttgtttt tataaaattt
     5641 atagaaaaac acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc
     5701 gtgtgctgtt tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc
     5761 accaacctag tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc
     5821 ccaatcccgc gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa
     5881 tgaaatggaa tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc
     5941 caagaacaca aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt
     6001 taaatccgtc agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca
     6061 atcctgggac gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat
     6121 tctgatcgac ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa
     6181 caaaagtaac aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt
     6241 tccataacgc aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt
     6301 catacgcacc taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc
     6361 ctagaccaac aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta
     6421 aaaaagaaat cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc
     6481 ctggcaagat aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt
     6541 tggttgtgta ccccacttct tgattttcca ttttttgcct tttttccact atttgcgcac
     6601 tccttctttc atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact
     6661 ttttcatagg tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta
     6721 cctccattat gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg
     6781 tattgtttcc cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct
     6841 gctaattcta ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca
     6901 aaattatatg cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc
     6961 atttccgcca aatatttgac accttgatca atactgtcag ctggattatc aatgatctca
     7021 gcttctccat gcagactttc cgagctctgc atgagatctg tcccttttcc ttttgattcg
     7081 gtcaatatta tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct
     7141 gttgttattt ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcat
     7201 aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata
     7261 gataatcgtt ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact
     7321 atttctttat cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg
     7381 aattttcctg tattttttga catcttacga aaatgatcgt tgtttttcag taaaaacaat
     7441 tgcccaatga ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa
     7501 aagaaatatg caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc
     7561 ttccctctct ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc
     7621 cctgtactcg aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg
     7681 acaaacgaag catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg
     7741 ttgctggaaa aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca
     7801 ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac
     7861 acgcttgtta aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg
     7921 gtatggttac tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt
     7981 ttgctgcatg acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg
     8041 atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact
     8101 ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat
     8161 cctgaacgct tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata
     8221 gaaggtgatt tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt
     8281 caaaacaatc tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg
     8341 taaaactcta tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag
     8401 cggactaatg ctattgattg gtcttatctc tgtgttacct atggttggtt atgatattat
     8461 tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga caagctggct
     8521 gatcaatacg atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc
     8581 tgaactatat ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt
     8641 cttgtttttg atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt
     8701 cacgccagtt gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata
     8761 ttttcctgtc gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc
     8821 acttaaaact caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt
     8881 tagtttcctt tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc
     8941 attattcatt gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag
     9001 tttcgatgta atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt
     9061 tgtgtggatc ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt
     9121 ctttttcttc aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca
     9181 attagcaaca gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat
     9241 gctagtactt tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt
     9301 gaacccattg aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt
     9361 aatcattatg ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt
     9421 cttgattgtt ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt
     9481 tttcttgtct attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca
     9541 attgatctat tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt
     9601 gctttacatc gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt
     9661 tatttccttt ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat
     9721 cgctgttagt ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat
     9781 tggcgaagcc tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc
     9841 cgatagtcaa ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga
     9901 accaactgtt cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc
     9961 ttctggtaag aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg
    10021 actatgctat ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga
    10081 attcggctac gatttcatta agatggggga agaagcttat gtagacctga atagttttac
    10141 aacttcaggg aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg
    10201 atttacattt gatgtcttac aaccaccatt ttctactgag caaatgagta ctttcaaaaa
    10261 tatctctgat aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc
    10321 tgaagattat ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt
    10381 cgcttttgca acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat
    10441 gcgatatgat tctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt
    10501 tgcctatatg aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa
    10561 tgtcggaact tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt
    10621 tggttctcat ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa
    10681 ctgggtctca cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc
    10741 tttgctgatt attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa
    10801 aaaatggata cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg
    10861 aataaaagag actgaagtaa aacgaaagtt catgcttcta caatctcttc ttatacaatt
    10921 gaagaacaaa gagcggtcca aacagaaatc ctgtttggac cgctctttta ttttaaacat
    10981 ttttcattac caaaatattc gagtcgcgtt tcttatgctt cgatctctgt aacgatacct
    11041 gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt
    11101 ggatggatca agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct
    11161 tctggcaatg tgattgttcc tgtaacatca gttgtgcgga agtagaactg tggacgatag
    11221 ttgttgaaga atggagtatg acgtccacct tcttcttttg tcaacacgta cacttctgct
    11281 ttgaattttg tatgtggagt aatggaacct ggtttagcaa gtacttgtcc acgttcgata
    11341 tcgtcacgtt ggatcccccg taacaacacg cctacgttat ccccagcttc cccgtaatct
    11401 aacgttttac ggaacatttc tacacctgtt acaactgctt tttgtgtttc aggtttgatc
    11461 cctacgatct ctacctcatc accgacacga acagcaccac gatcaatacg acctgaggca
    11521 acagttcctc gacctgtaat tgagaagacg tcttccactg gcaatagtaa tggtttatct
    11581 gtatcacgtt ctggtgttgg gatatattca tctactgtat ccatcaattc cataatagca
    11641 gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat cacaggagtg
    11701 tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa cttccatttc taccaaatcg
    11761 atcaattctt catcatcgac taaatcaact ttgttcaaga aaacaatcag gtattttaca
    11821 ccaacttggc gtgacaataa aatgtgttca cgtgtttgag gcatcggacc gtcagttgca
    11881 gaaacaacta agattgcacc atccatttgt gctgcccctg taatcatgtt tttcacatag
    11941 tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc atattctacg
    12001 tgtgcggtat tgatcgtaat cccacgttca cgttcttctg gagctgcatc aatactagca
    12061 tagtcttgag gatttgctag gccttttttg cctaatactg tagtgatcgc agcagttaaa
    12121 gtagttttcc catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg
    12181 tcataatgtt cttttgccat aatttacaaa cctcctaaat aatatagatc actgaaaaat
    12241 ttcagctctc acctttttat tatagtcttt ttttgactaa atcaaaaaga atacgtccga
    12301 cttacttttt gtaagattcg aactaagctt attatacgtc aatattggtc aaagtcaact
    12361 aagagcactc gtctgtctta tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt
    12421 ttttgagaaa aacttcaaga tagtccttaa ctttcttttg aagcaataag aacaaaatga
    12481 ggaaaagttt aaaattcagc tgttgccaac aactttcaat agacgaaatc ttctatttcg
    12541 ttaataataa ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta
    12601 ggcatatttc tcggcggaag ttttgcagct tatcattatt tttacggcgg cgaagcttat
    12661 tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc aagcagataa cggagagaaa
    12721 ttcacgactt atacctacaa gcaagcggct tataataaag atggtgaaaa aaaacaggtg
    12781 acgcttcgcg aagaaagaga acatccatta aagatgaatg cgtatcttaa actcaaagtc
    12841 aatcctcgta aaggtgtatt aagctgggaa gaaataaagg cttctgaagt gcctaaaaat
    12901 gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat tttattagat aaaa
//
LOCUS       DACELF010000077        12896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.080, whole genome shotgun sequence.
ACCESSION   DACELF010000077 DACELF010000000
VERSION     DACELF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.080"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..366)
                     /locus_tag="IW687_002115"
     CDS             complement(<1..366)
                     /locus_tag="IW687_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0951368.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAG"
     gene            556..825
                     /gene="rpsN"
                     /locus_tag="IW687_002116"
     CDS             556..825
                     /gene="rpsN"
                     /locus_tag="IW687_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0951369.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1001..1363)
                     /locus_tag="IW687_002117"
     CDS             complement(1001..1363)
                     /locus_tag="IW687_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0951370.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(1374..2495)
                     /locus_tag="IW687_002118"
     CDS             complement(1374..2495)
                     /locus_tag="IW687_002118"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0951371.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(2511..2861)
                     /locus_tag="IW687_002119"
     CDS             complement(2511..2861)
                     /locus_tag="IW687_002119"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0951372.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2991..4502)
                     /locus_tag="IW687_002120"
     CDS             complement(2991..4502)
                     /locus_tag="IW687_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0951373.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(4824..6176)
                     /locus_tag="IW687_002121"
     CDS             complement(4824..6176)
                     /locus_tag="IW687_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0951374.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6329..7405)
                     /locus_tag="IW687_002122"
     CDS             complement(6329..7405)
                     /locus_tag="IW687_002122"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0951375.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            7601..9346
                     /locus_tag="IW687_002123"
     CDS             7601..9346
                     /locus_tag="IW687_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0951376.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(9595..10062)
                     /locus_tag="IW687_002124"
     CDS             complement(9595..10062)
                     /locus_tag="IW687_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0951377.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10046..11419)
                     /locus_tag="IW687_002125"
     CDS             complement(10046..11419)
                     /locus_tag="IW687_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951378.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(11740..12228)
                     /locus_tag="IW687_002126"
     CDS             complement(11740..12228)
                     /locus_tag="IW687_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951379.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12250..12852)
                     /locus_tag="IW687_002127"
     CDS             complement(12250..12852)
                     /locus_tag="IW687_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0951380.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3710 a         2800 c         2332 g         4054 t
ORIGIN      
        1 tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa
       61 cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg
      121 aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac
      181 ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata
      241 aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg
      301 atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct
      361 tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa
      421 aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta
      481 acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat
      541 tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa
      601 acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc
      661 ttgcgcaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttgatcgat
      721 ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta
      781 gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt
      841 aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct
      901 tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc
      961 cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca
     1021 gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat
     1081 ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca
     1141 ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaata tgagtcggaa
     1201 gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt
     1261 tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga
     1321 ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct
     1381 tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct
     1441 aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc
     1501 acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca
     1561 cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt
     1621 ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata
     1681 tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg aattaattca
     1741 gaaaccagct caagtgctgg ctgcagtgga taaacttccg gtagtgcgtg cccagaggga
     1801 ttcagtccgt acattgctac tccataacga atcatattgc cgattgtttc atcatgccat
     1861 aaagccgttg cactattgct gacatgtata tatcgaggaa gttcgggaag tttttttagc
     1921 acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta
     1981 gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct
     2041 gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt
     2101 agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcctgc
     2161 aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg
     2221 tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg
     2281 actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca
     2341 gctgtttcaa tagctccatg accgtatccg tttgctttta ctactgcaaa caattctttt
     2401 ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc
     2461 actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc
     2521 aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tactttgcct
     2581 tcatgaggtg aacgcgtcac aacaggctga ccgttttcat tttttaaaat ctcgacatct
     2641 tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg
     2701 taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt
     2761 tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc
     2821 ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat
     2881 aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa
     2941 ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta
     3001 tttgaacgga ttacaaatcc gcgtttttta tcgttgtgac gtttaccgcc atctttttgg
     3061 ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca
     3121 cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc
     3181 attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct
     3241 gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct
     3301 gctgtttgca ggtacttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact
     3361 gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc
     3421 cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa
     3481 gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt
     3541 tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca
     3601 cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat
     3661 acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt
     3721 gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt
     3781 agttcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt
     3841 gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca
     3901 tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct
     3961 gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc
     4021 aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca
     4081 agtttcaatg tgtggcggtt gatgtggtca agcatacgtc ctggtgtacc cacaacgata
     4141 tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct
     4201 tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct
     4261 aactcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt
     4321 tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata
     4381 acgtcttttc cagctaatgc tagtggaatc gtttctgatt ggataggtgt tgcttcttca
     4441 aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc
     4501 aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat
     4561 ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc
     4621 ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa
     4681 acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg
     4741 gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc
     4801 gttttctgca agtttacaga tttttatttc atttgctgaa gctgttctac cacttcaaat
     4861 aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttt
     4921 acagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat
     4981 ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg
     5041 aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct
     5101 aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggtag atttttaaaa
     5161 gtatctaata ccagtcccat ggctgttgga tttgcattat agacatcgct caaaatagct
     5221 gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc
     5281 ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa
     5341 gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca
     5401 aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata
     5461 ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct
     5521 gcgggcacaa cgagtaatcc gtctggtaca agtccgtcag tgatttccat tttggcttta
     5581 gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct
     5641 gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc
     5701 atttccaaga cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca
     5761 atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac
     5821 gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgca
     5881 cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca
     5941 ggaataactg gaaaatcttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat
     6001 gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga
     6061 acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc
     6121 agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag
     6181 atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact
     6241 ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca
     6301 atcactccag cttcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct
     6361 gatggtatcg attcattcct aattgaatca gttcttcaat caaatcaccg tatttcaagc
     6421 ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta
     6481 attcattcag gaataattcg tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc
     6541 ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt
     6601 ccggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttg gcttcataat
     6661 catagaatgc tacgtctttt acgacttcgc caggcaaagt cgtccgaaca tcttcatttc
     6721 ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat
     6781 catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct gcctttgtaa
     6841 tgccgacact agaacccata ttcgcaggtt tgacaaacat cggataaagc aaagaacctt
     6901 cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat
     6961 aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg
     7021 cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc
     7081 cttggatcgt tccatcttcc ccgtttggcc cgtgtaaaac tggaaaaacg atggctcctt
     7141 cttctttgat ttctcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac
     7201 ttgggtccca tgaaagatgc aagacatctt tgctagcagg tttttctgtt agtaatggac
     7261 ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat
     7321 aaatggcatt taaaactgaa aatgcggaaa gaatggaaac ttcatgctct gcgctgcgtc
     7381 cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga
     7441 tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg
     7501 agttggtttc taaaagaaca aacgtcattt attttttgtc agctagatta tttgtcccgt
     7561 tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg
     7621 tagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt
     7681 acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat
     7741 taagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac
     7801 attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat
     7861 cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcca aatcgccttt
     7921 tgcctctgct tctatggggc aggctcatcg cgccaagctg aagaacggtg actcggtcgt
     7981 cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc aactatttga
     8041 acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt
     8101 actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa
     8161 tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta
     8221 tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa
     8281 ccatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca
     8341 gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg tatttgatga
     8401 tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac taaaaaaaga
     8461 agaacagaca caagctagcc gaaaaactga aatagtccat gaaaaagagt tcggttcctt
     8521 tgcttttcga gcctccactt ctacagaaga ccctgttgcg ccatatacca tcaactacat
     8581 cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt
     8641 tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca
     8701 gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca
     8761 gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac acgtcgtgac
     8821 gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt
     8881 tgggacatta gaaggggtga tccgcgtcct tgaccctgaa gtttccctca tggaagtcgc
     8941 ttcgcctttt gcccaacatt attttctcac tcatctggat gtagaagata cattgaaaca
     9001 aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca
     9061 ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca
     9121 agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt
     9181 agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga
     9241 ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt
     9301 ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac
     9361 agaaagaatc agcttcaaga aataagaagt gattttgcat aaattgtttc tcaaattttt
     9421 gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa gcaccgagaa taaaaaatgt
     9481 gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa aggaacatgg agcacattta
     9541 tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct
     9601 actatcttct gtacttggta aaccttggtc aattgccttt tttaacgctt gacaaaaaga
     9661 catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa
     9721 ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt
     9781 tccagagtga tggaacactc ggacgccctt cggcaataca agtgaagcta aaccataatc
     9841 ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc
     9901 agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg
     9961 gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc
    10021 ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc
    10081 ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca
    10141 agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata
    10201 gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact
    10261 ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg
    10321 gaatagctgc atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc
    10381 agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag
    10441 aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc
    10501 atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg
    10561 aaagtttttc taatccgtac attatgccaa cataaaaact gctaaataaa aaaaggaaat
    10621 tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct
    10681 tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag
    10741 caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc
    10801 caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt
    10861 tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa
    10921 gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat
    10981 acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac
    11041 cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca
    11101 gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa
    11161 gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac
    11221 caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa
    11281 atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa
    11341 taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc
    11401 gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt
    11461 cgattttagc attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa
    11521 tacagaaaga agcataagct tctttctgca tttttacggt acttgattac tcaagcagac
    11581 aagcccttct ccacaaaagt cttgcccgac acccctgcat ccggcaggtg ataaagatag
    11641 aaatcaatta tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc
    11701 gacccgtcgc ataaatttac tttaacacag ttcctatgtt tatgcgagag cttttttcat
    11761 tttataatcg accatttgaa atccttgacc gatatctgaa ataagttctt ctgtaatctc
    11821 aaatccttca tgcttataaa cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa
    11881 gatctctctt tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt
    11941 caatccttga ccacgtactt tatttagcaa atataattta cttaaataaa tttttccatt
    12001 tatctcttca taagcagtgt aaccgacagt cttttcttcg ctttttaata aaaagtagga
    12061 cacaccttct gtcagttctt tttgaatctg tttttttgac tgataagttt tcagcatgta
    12121 atccacttgt ttttgaccaa tgatcggcac aaaagcttcc ggccatactt cttgtatgac
    12181 tgccatcagc tcatccagtt cattttctgt tacttttatt ttttccatac acccacgtcc
    12241 ttttttacat tataaatgac cttgcgcttg atggaataaa aagagcatga tcatgctata
    12301 cacacccacc gaccacggtt tcaagaaaat cacaatctgc ataaattccc taaaagataa
    12361 tttcgtcaaa ccagctacta gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt
    12421 aacaactagt acttttttaa tatctttcgt ttttcttgtc agcaattcat caaatttctg
    12481 atactttttt ggcgataaaa taagctggac aaaggctacg ccatagtaac ggacaagcaa
    12541 aaatccgatt gcttccccaa tcactaggcc aatacaacta tataaaagcc caaatccatt
    12601 gccaaacatc agcatgcccg ctactgagga gatccctccc ggcaaaatag gaatgatcac
    12661 ttggatcact tgcaacagaa caaatatcat cactgcatac ttgccaaatt gctggataaa
    12721 cgcttgcaaa gacgtgctcg atcgaaaaat cccatgatga taagcataaa gaatcaacac
    12781 aaagaaaaaa aacagaccaa taatgggcat gatctgaaga catttttgta aaactgatcc
    12841 ttttttagac atctatattc cccacatctt ttttcattta ttaaaactgc ctcctt
//
LOCUS       DACELF010000078        12377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.081, whole genome shotgun sequence.
ACCESSION   DACELF010000078 DACELF010000000
VERSION     DACELF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.081"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW687_002128"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW687_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0951381.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW687_002129"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW687_002129"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0951382.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW687_002130"
     CDS             complement(2169..2906)
                     /locus_tag="IW687_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0951383.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW687_002131"
     CDS             complement(2997..3524)
                     /locus_tag="IW687_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0951384.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW687_002132"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW687_002132"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0951385.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW687_002133"
     CDS             complement(4401..4886)
                     /locus_tag="IW687_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0951386.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW687_002134"
     CDS             complement(5039..5236)
                     /locus_tag="IW687_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951387.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW687_002135"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW687_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0951388.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW687_002136"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW687_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0951389.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW687_002137"
     CDS             9211..9423
                     /locus_tag="IW687_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0951390.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW687_002138"
     CDS             9425..11104
                     /locus_tag="IW687_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0951391.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW687_002139"
     CDS             complement(11358..11558)
                     /locus_tag="IW687_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0951392.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW687_002140"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW687_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IW687_002141"
     tRNA            complement(12249..12322)
                     /locus_tag="IW687_002141"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
BASE COUNT         3616 a         2667 c         2148 g         3946 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga ttccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaa
//
LOCUS       DACELF010000079        12186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.082, whole genome shotgun sequence.
ACCESSION   DACELF010000079 DACELF010000000
VERSION     DACELF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.082"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1255
                     /locus_tag="IW687_002142"
     CDS             <1..1255
                     /locus_tag="IW687_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0951393.1"
                     /translation="KAFMSALTLGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPA
                     LDLGWTGAASITWSWSYAFIFFAVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLV
                     YYVSGSLAAGFLTAMVQVILELKLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFN
                     MIMDKIPALNKRADTNALKKKIGIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTA
                     TALSLFPMISKMFMQSLSPLADAMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAIL
                     LVPVFIGYAIILPGNQVLPLAGIINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGA
                     TYLAPILSDLAEKSGAVEGIKKGQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFL
                     GLFVLLYKYMIKAKVPSERYAELQK"
     gene            1277..1537
                     /locus_tag="IW687_002143"
     CDS             1277..1537
                     /locus_tag="IW687_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951394.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1551..2303
                     /locus_tag="IW687_002144"
     CDS             1551..2303
                     /locus_tag="IW687_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0951395.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2397..3002)
                     /locus_tag="IW687_002145"
     CDS             complement(2397..3002)
                     /locus_tag="IW687_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0951396.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3055..4029)
                     /locus_tag="IW687_002146"
     CDS             complement(3055..4029)
                     /locus_tag="IW687_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0951397.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4407..5186)
                     /locus_tag="IW687_002147"
     CDS             complement(4407..5186)
                     /locus_tag="IW687_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0951398.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5253..6524)
                     /gene="celB"
                     /locus_tag="IW687_002148"
     CDS             complement(5253..6524)
                     /gene="celB"
                     /locus_tag="IW687_002148"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0951399.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6677..8074
                     /locus_tag="IW687_002149"
     CDS             6677..8074
                     /locus_tag="IW687_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0951400.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8155..8595
                     /locus_tag="IW687_002150"
     CDS             8155..8595
                     /locus_tag="IW687_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0951401.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8743..9069
                     /locus_tag="IW687_002151"
     CDS             8743..9069
                     /locus_tag="IW687_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0951402.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9070..9819
                     /locus_tag="IW687_002152"
     CDS             9070..9819
                     /locus_tag="IW687_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951403.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(9899..10354)
                     /locus_tag="IW687_002153"
     CDS             complement(9899..10354)
                     /locus_tag="IW687_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0951404.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(10781..11056)
                     /locus_tag="IW687_002154"
     CDS             complement(10781..11056)
                     /locus_tag="IW687_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951405.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11350..12165
                     /gene="thiD"
                     /locus_tag="IW687_002155"
     CDS             11350..12165
                     /gene="thiD"
                     /locus_tag="IW687_002155"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0951406.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         3892 a         2115 c         2480 g         3699 t
ORIGIN      
        1 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
       61 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
      121 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
      181 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
      241 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
      301 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
      361 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
      421 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
      481 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
      541 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
      601 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
      661 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
      721 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
      781 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
      841 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
      901 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
      961 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     1021 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     1081 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     1141 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     1201 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     1261 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     1321 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     1381 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     1441 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     1501 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     1561 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     1621 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     1681 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     1741 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     1801 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     1861 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     1921 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     1981 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     2041 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     2101 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     2161 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     2221 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     2281 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     2341 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     2401 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     2461 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     2521 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     2581 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     2641 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     2701 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     2761 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     2821 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     2881 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     2941 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     3001 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     3061 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     3121 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     3181 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     3241 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     3301 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     3361 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     3421 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     3481 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     3541 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     3601 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     3661 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     3721 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     3781 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     3841 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     3901 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     3961 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     4021 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     4081 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     4141 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     4201 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     4261 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     4321 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     4381 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     4441 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     4501 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     4561 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     4621 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     4681 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     4741 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     4801 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     4861 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     4921 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     4981 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     5041 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     5101 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     5161 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     5221 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     5281 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     5341 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     5401 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     5461 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     5521 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     5581 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     5641 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     5701 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     5761 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     5821 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     5881 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     5941 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     6001 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     6061 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     6121 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     6181 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     6241 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     6301 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     6361 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     6421 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     6481 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     6541 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     6601 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     6661 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     6721 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     6781 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     6841 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     6901 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     6961 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     7021 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     7081 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     7141 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     7201 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     7261 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     7321 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     7381 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     7441 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     7501 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     7561 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     7621 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     7681 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     7741 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     7801 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     7861 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     7921 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     7981 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     8041 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     8101 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     8161 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     8221 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     8281 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     8341 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     8401 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     8461 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     8521 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     8581 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     8641 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     8701 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     8761 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     8821 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     8881 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     8941 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
     9001 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
     9061 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
     9121 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
     9181 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
     9241 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
     9301 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
     9361 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
     9421 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
     9481 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
     9541 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
     9601 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
     9661 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
     9721 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
     9781 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
     9841 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
     9901 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
     9961 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    10021 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    10081 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    10141 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    10201 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    10261 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    10321 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    10381 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    10441 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaaacaac
    10501 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    10561 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    10621 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    10681 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
    10741 tattacagaa catttttaat taataaagaa cagttagaag ttaaaattgc tgattcacat
    10801 agagtttatg acttcttgga aattcttttt caatcagttt tttcatctgt ttatgaaatt
    10861 cttttttttc agtaaacata aaggtttggg tatcgtaacc ttcttcatgt aggaaataga
    10921 ttattccttt ttctgtgttt tgcgtataag cttttatact cctgtctttt ttgaaagaac
    10981 agatcgtata gccatctatt tgctctggta gtcgtttgat tttcttcaaa atgacttttt
    11041 tatcctcaag tttcattttt ttcacctgac tttatttttt tcatatggaa atcatagcgc
    11101 ctttggttat atgaaaacaa gaaggatact gaaatatata taaatcaaat agtggttatt
    11161 tattattaga atagcgaata caaataaacg accactattt tctgatttat tgcaaatgtt
    11221 ttttcaaata aatttgatta ccaagaaaag gtagtggtat atttactgag ggatactttg
    11281 aaaaacatat gatagaaaca gtaacagtat tatgaaaaat ctttttataa aaatggagga
    11341 atcgatttga tggaagatca gtatgtacaa gcaatgacga tcgcaggatc agatagcgat
    11401 ggaagcgcag ggatgcaagc agatatgcat acattcttta caagaaatgt ttacggtgtt
    11461 tctgtcatga ccgcatgtgt tgcaggtaat tcttacggaa tcggagcaag tgtgacattg
    11521 ccaacagatt ttatcgacaa agaatttgaa ctgattgcga aggactttca aatacgtgca
    11581 gctaaaacag gtatgctagc agattcaaag ttgattgaga cagttgtaaa aaattaccaa
    11641 aaatatgatt ttggaccatt agtcgtggat cccgtaatcg taacgaaaca tggaaatctt
    11701 ttactagaag aatctgcttt gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta
    11761 ctgacaccta atttttatga agcagaaaag ctgactggac ggacattagc agctgaagaa
    11821 gattttgtgc aagcagcaaa agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa
    11881 ggacatcatg ctaaaaatgc gcagcaagaa acagtcaatg actacgtatt gttcgaatca
    11941 ggaaaatcat tttggttaaa tgcaccattt tatcctaccg accggatcaa cggaactggc
    12001 gattcgctat ctgcatgtat tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg
    12061 atccgattgg caaaagacta tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac
    12121 aaatatggcc ccatcaatca ttgggcagct ggtgaattag agtaaaagaa agaagttgtg
    12181 aaaaag
//
LOCUS       DACELF010000080        12055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.083, whole genome shotgun sequence.
ACCESSION   DACELF010000080 DACELF010000000
VERSION     DACELF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.083"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..388)
                     /gene="frr"
                     /locus_tag="IW687_002156"
     CDS             complement(<1..388)
                     /gene="frr"
                     /locus_tag="IW687_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0951407.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIR"
     gene            complement(391..1113)
                     /locus_tag="IW687_002157"
     CDS             complement(391..1113)
                     /locus_tag="IW687_002157"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0951408.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1249..2130)
                     /locus_tag="IW687_002158"
     CDS             complement(1249..2130)
                     /locus_tag="IW687_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0951409.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2229..3011)
                     /gene="rpsB"
                     /locus_tag="IW687_002159"
     CDS             complement(2229..3011)
                     /gene="rpsB"
                     /locus_tag="IW687_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0951410.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3370..3849
                     /locus_tag="IW687_002160"
     CDS             3370..3849
                     /locus_tag="IW687_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0951411.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4066..5157
                     /gene="phnW"
                     /locus_tag="IW687_002161"
     CDS             4066..5157
                     /gene="phnW"
                     /locus_tag="IW687_002161"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0951412.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5150..5278
                     /locus_tag="IW687_002162"
     CDS             5150..5278
                     /locus_tag="IW687_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0951413.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5282..5827
                     /locus_tag="IW687_002163"
     CDS             5282..5827
                     /locus_tag="IW687_002163"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0951414.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6280..7971)
                     /locus_tag="IW687_002164"
     CDS             complement(6280..7971)
                     /locus_tag="IW687_002164"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0951415.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8391..9338)
                     /gene="arcC"
                     /locus_tag="IW687_002165"
     CDS             complement(8391..9338)
                     /gene="arcC"
                     /locus_tag="IW687_002165"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0951416.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9453..10472)
                     /gene="argF"
                     /locus_tag="IW687_002166"
     CDS             complement(9453..10472)
                     /gene="argF"
                     /locus_tag="IW687_002166"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0951417.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10563..11792)
                     /gene="arcA"
                     /locus_tag="IW687_002167"
     CDS             complement(10563..11792)
                     /gene="arcA"
                     /locus_tag="IW687_002167"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0951418.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3316 a         2493 c         2177 g         4069 t
ORIGIN      
        1 tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct aattcttttc
       61 tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac
      121 tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa
      181 aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg
      241 gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc
      301 ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt
      361 ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt
      421 ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg
      481 aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc
      541 aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc
      601 agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc
      661 tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc
      721 aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc
      781 tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa
      841 cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg
      901 agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccgcccac
      961 aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt
     1021 gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc cactcaattt
     1081 taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc
     1141 cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa
     1201 aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac
     1261 ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc
     1321 gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat
     1381 atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa
     1441 gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc
     1501 tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata
     1561 acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt
     1621 tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc
     1681 agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac
     1741 ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga ttttcattgc
     1801 agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc
     1861 ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc
     1921 agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt
     1981 cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc
     2041 tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc
     2101 ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt
     2161 aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc
     2221 attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt
     2281 gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt
     2341 ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg
     2401 tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct
     2461 acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca
     2521 acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca
     2581 agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt
     2641 ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc
     2701 cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt
     2761 gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg
     2821 ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt
     2881 tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga
     2941 ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa
     3001 attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc
     3061 ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat
     3121 gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac
     3181 ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta
     3241 gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg
     3301 aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg
     3361 agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc
     3421 ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg
     3481 caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat
     3541 ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa
     3601 gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt
     3661 ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag
     3721 atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg
     3781 gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca
     3841 cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt
     3901 ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt attcaaattt
     3961 ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc
     4021 ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta
     4081 acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt
     4141 acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt
     4201 gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag ctccttcggg
     4261 gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac
     4321 ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt
     4381 tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag
     4441 aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat
     4501 aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat agtggacgcg
     4561 atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga ttttttgatt
     4621 tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa
     4681 gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct atatgaccag
     4741 taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt
     4801 ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat
     4861 tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat ggggttccag
     4921 cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg
     4981 cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt gatctatccc
     5041 gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa
     5101 gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac
     5161 tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc
     5221 ttttagaaat gcttttctag aaaaaggaac ccaactgact gacaaagaaa tccgctaacc
     5281 gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa
     5341 acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca
     5401 acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac
     5461 attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca cgaatggatt
     5521 taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt attcaccaga
     5581 cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca
     5641 taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac
     5701 ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg
     5761 tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaagat
     5821 cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat ttgtgtaaac
     5881 aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga atgactttta
     5941 tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac agccaagcaa
     6001 aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
     6061 caccgtttat tcgaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt
     6121 tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact
     6181 ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta
     6241 atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg
     6301 aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa
     6361 gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa
     6421 tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc
     6481 agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc
     6541 attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac
     6601 tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc
     6661 ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc
     6721 cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct
     6781 gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt
     6841 tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg
     6901 catgtcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg
     6961 atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg
     7021 gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat
     7081 caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt
     7141 cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc
     7201 ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt
     7261 catcgattca tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag
     7321 acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg
     7381 gaattggacg tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt
     7441 accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg
     7501 gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat
     7561 aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc
     7621 gattggtact gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac
     7681 tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg
     7741 tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc
     7801 gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc
     7861 tccgtgttca gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc
     7921 atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc
     7981 tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta
     8041 tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca
     8101 gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc
     8161 gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa
     8221 gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa
     8281 aaaacgaaag gctagtcgtt tcataaaaaa aaaatccaga tggcctaaag gagaagaaat
     8341 accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct
     8401 acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg
     8461 attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcttctac tttcggaagc
     8521 atgcttcctg gagcgaattg cttttcatca atgtattgtt tcatttctga aacagtaaca
     8581 gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc
     8641 aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta
     8701 tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg
     8761 ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct
     8821 ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct
     8881 ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat
     8941 ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga
     9001 gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggcg
     9061 ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt
     9121 gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga
     9181 ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt
     9241 gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagatggca
     9301 ttgcccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt
     9361 ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga
     9421 ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct
     9481 aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga
     9541 cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt
     9601 tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag
     9661 aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt
     9721 ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca
     9781 gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt
     9841 gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat
     9901 tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag
     9961 ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca
    10021 aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc
    10081 aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg
    10141 aatccacgga attcgattcc gtcaaacata cgtcctagta ctttagctgt atcttctgtg
    10201 gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga
    10261 tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc
    10321 gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag
    10381 tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat
    10441 aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt
    10501 atttaagatt aaagaggaaa tcccgacggc agctgacagt aagactgccg tcaggaaaca
    10561 aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacgacct
    10621 cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg
    10681 ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt
    10741 gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc
    10801 agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga
    10861 gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta
    10921 ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc
    10981 aaatgcatca ttgcacggtt gttcggaatt ttgattgcca acactttttc aaatcctgaa
    11041 ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa
    11101 atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct
    11161 ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc
    11221 aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact
    11281 gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg
    11341 tccatgtaaa atggatagtc atcgtctgct gacagatcat atagactgct agaacgtact
    11401 tcgatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt
    11461 gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc
    11521 aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat
    11581 ttttccaagt aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt
    11641 tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct
    11701 ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta aaactgtctt taatttccct
    11761 atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac
    11821 atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatgaga
    11881 cattcctcac attaatattc gataattaaa taattaaacg tttttctatt tttaagcgta
    11941 atttattcat tttttattct gtatacactt tcaaaaaaat atttattcat tgaaaaaatt
    12001 tttttgaata aattaaaaaa ataaattata ttatcgttgt tagcgtttac tatat
//
LOCUS       DACELF010000081        11891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.084, whole genome shotgun sequence.
ACCESSION   DACELF010000081 DACELF010000000
VERSION     DACELF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.084"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            166..408
                     /gene="yaaA"
                     /locus_tag="IW687_002168"
     CDS             166..408
                     /gene="yaaA"
                     /locus_tag="IW687_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0951419.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            395..1519
                     /gene="recF"
                     /locus_tag="IW687_002169"
     CDS             395..1519
                     /gene="recF"
                     /locus_tag="IW687_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0951420.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1516..3462
                     /gene="gyrB"
                     /locus_tag="IW687_002170"
     CDS             1516..3462
                     /gene="gyrB"
                     /locus_tag="IW687_002170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0951421.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3482..5953
                     /gene="gyrA"
                     /locus_tag="IW687_002171"
     CDS             3482..5953
                     /gene="gyrA"
                     /locus_tag="IW687_002171"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0951422.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6189..6488
                     /locus_tag="IW687_002172"
     CDS             6189..6488
                     /locus_tag="IW687_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0951423.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6538..7050
                     /gene="ssb"
                     /locus_tag="IW687_002173"
     CDS             6538..7050
                     /gene="ssb"
                     /locus_tag="IW687_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0951424.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7076..7312
                     /gene="rpsR"
                     /locus_tag="IW687_002174"
     CDS             7076..7312
                     /gene="rpsR"
                     /locus_tag="IW687_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0951425.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7455..9428
                     /locus_tag="IW687_002175"
     CDS             7455..9428
                     /locus_tag="IW687_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0951426.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9434..9886
                     /gene="rplI"
                     /locus_tag="IW687_002176"
     CDS             9434..9886
                     /gene="rplI"
                     /locus_tag="IW687_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0951427.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10160..11527
                     /gene="dnaB"
                     /locus_tag="IW687_002177"
     CDS             10160..11527
                     /gene="dnaB"
                     /locus_tag="IW687_002177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0951428.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3978 a         2149 c         2632 g         3132 t
ORIGIN      
        1 agaaggccct aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat cacctttttt
       61 agattaaatc gtcttatatg gaaaaatata tggataaatt tgttttattg cgaaaaaaaa
      121 ggtataatag agtatacttt taataatgaa aatgagggat gggcattgaa acaacaagtc
      181 attctaaata cggaattcat gacgttgggc caaatgttga aagaagtcac agttatcggt
      241 agtggcggac aagcaaaatg gtatttggca gaaaacactg tgttagtcga tggcgaacca
      301 gaaaatcgtc gtggacgcaa actttatccc ggcatgatgg ttgaagttcc agaagtagga
      361 acttttttta tggtaaaaaa aggaaacgac gataatgagg ctgaataagc tgtatttaaa
      421 aaattatcga aattacgaag agctgaacat cgaattttcc aagaacctcg tcatcttttt
      481 aggagaaaat gcgcaaggaa aaacgaatct tttggaaagt atctacgtat tagctatgac
      541 gcgaagtcat cggacaagta gtgagcagga attgattggc tggactgacg atcaggcgat
      601 gattcaagga gagatcacaa aaggttcatc cactcttcca ttagaaatct tattatctaa
      661 aaaaggccga aaaaccaaga tcaaccatat cgaacagaaa aagttaagta cttacgtggg
      721 acaaatgaat gtcattttat ttgcaccaga agatctttct ttagtaaaag gcagcccgca
      781 gatcaggcgg aagtttttag atatggaatt aggccagatc aatccaatct atctttatga
      841 tcttgtccaa taccaagcaa ttttgaaaca aagaaaccag tacttgaagc aattgaatga
      901 aaaaaaacaa actgatttgc tttatttgga tattttgtcc gaacaactag cagcgtttgg
      961 cagcaaagta ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt gggctaattc
     1021 tttgcatcag caaatcaccc atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt
     1081 cgattcttta gaaacacata cgcaagagca aatccaagaa caatttctcg cacttttgaa
     1141 ccaaaataaa aagaaagacc tttttcgggg aacaacgaca gttggtcctc atcgtgacga
     1201 tctttccttt tttattaatc agaaaaatgt gcagacttat ggatcgcaag gacagcaaag
     1261 aacaactgca ctaagtgtca aattagcaga aatcgatttg attaaagaag agacagggga
     1321 atatccaatc cttttgttag atgatgtgat gagcgagctg gatgataatc gtcaactcca
     1381 tctgctagaa acaatcgaag gaaaagttca aacgttctta acaacaacga cacttgacca
     1441 tgtaaaaaat aaaatgactg ttgaacctga gattttttat gttcaacaag ggaaactaaa
     1501 aggagaagaa actgaatgac agaagaaaga agtttagtag aacgcgctaa agagtatgat
     1561 gccagtcaga ttcaagtact tgaaggactt gaagccgttc gtaaacgacc tggcatgtat
     1621 atcggttcga ctagttctga gggtctgcat caccttgttt gggaaattgt cgataattcg
     1681 atcgatgaag tattagcagg ttttgcaacg aaaatccatg tgattattga aaaagacaac
     1741 agtatcaccg ttattgacga cggtcggggg atacctgtag atatccaggc aaaaactgga
     1801 cgtcctgcag ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt cggcggtggt
     1861 gggtacaaag tttctggtgg acttcacgga gtaggatctt ctgttgtaaa cgcgctttct
     1921 acacagcttg atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt
     1981 ggagctgtag tggacgactt aaagattatc gaacaaacgg atcgccatgg aacaacggtt
     2041 cactttacgc cagaccctga aatttttaca gagacaactg agtttgattt ttcaaaattg
     2101 gctacccgga tccgtgaact tgctttcttg aaccgcggga tgcgtatatc gattgaagac
     2161 aaacgagaag aagaaccagt catcaatgaa taccattatg acggcgggat caaaagttat
     2221 gtcgaatact tgaatgcaaa caaaaccgtg atttttccag aacctgtata tttggaaggt
     2281 gaacagcaag atatcgcagt tgaagtctct atgcaatata cagacggcta ccattccaat
     2341 atcatgagtt ttgccaacaa tatccatacg tatgaaggtg gaacacatga atcaggattc
     2401 aagactgctt taactcgtgt gataaatgat tatgcccgaa aacaaaagct gatgaaagaa
     2461 aatgacgaca acttgaccgg tgaagacgtg cgagaaggat tgacagctgt tatttctatc
     2521 aagcatcctg accctcaatt cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta
     2581 cggacagtca ctgatcggtt atttagcgaa catttcatga aattcttgct ggaaaaccca
     2641 agtgtgggcc ggcaaattgt tgaaaaagga ttactagctt ccagagctcg tctagcagcg
     2701 aaacgtgcgc gtgaagtaac gagacgtaaa ggtgctttag agattagtaa tctgccagga
     2761 aaattagcgg actgctctag caacgatcct gaaaaatgcg aattgttcat cgtcgaagga
     2821 gattcggccg gcggatcagc taagcaaggt cgtaatcgag agtttcaagc catcttgcct
     2881 attcgtggga aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa
     2941 gagatccggt ctttatttac tgctatgggt acagggtttg gagaagattt tgatgtatcg
     3001 aaagctcgct accacaaatt agttatcatg actgatgccg atgtcgacgg cgcgcatatc
     3061 cgtacattgc tgctgactct tttttatcgt tacatgcgtc ctattgttga agctggatac
     3121 gtctatattg ctcagccacc tttatacggg gtaaaacaag ggaagaatat tacgtatgta
     3181 caaccaggaa aaaatgcaga agaagagttg aaacaagtcg ttgcttcact tcctgcctca
     3241 ccaaaaccaa gcgttcagcg ttataaagga cttggagaaa tggatgatca tcaactatgg
     3301 gaaacaacta tggacccaag caatcggatg atggcccgcg tcagtgtgga cgatgctatt
     3361 gcagcagatc agatatttga aatgttgatg ggggatcgag tagaaccccg tcgtgcattc
     3421 atcgaggaaa atgcacacta tgtgaagaac ttagatattt aatcatgaag gaggaaaaag
     3481 aatgagtgaa gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga
     3541 ctccttcatc gattatgcga tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga
     3601 tggtctgaaa cccgttcatc gccgtatttt atacggcatg aacgaattag gtgtaacacc
     3661 ggataaagcc cataaaaaat ctgccagaat cgtcggagat gtcatgggta aataccatcc
     3721 tcatggagac cgtgcgatct atgagtcaat ggtacggatg gcacagccat tcagctaccg
     3781 ttatatgcta gtggatggtc atggaaactt tggttctgtt gacggtgacg gtgccgccgc
     3841 tatgcgttat accgaagcac ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa
     3901 taagaacact gttgatttcc aaagcaacta tgatgataca gaaaaagaac cagttgtact
     3961 acctgctcgc ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg taggtatggc
     4021 cacgaatatc ccacctcata acttgtctga ggtagtagcg gctatcgatt tgctgatgga
     4081 taatcctgat gtgacgacca atgaattgat ggaagtatta cctggaccag attttccaac
     4141 aggtggtttg gttatgggga aatcaggaat ccgaagagcg tatgaaacag gtaagggttc
     4201 tattaccgta cgtgcaaaag ttgagattac tgagatgcca aatggtaaag aacggatcat
     4261 cgtgacagaa ttgccttata tggtcaataa agcaaaattg atcgaacgga tttctgaact
     4321 gcacagagac aagcgtatcg aagggatcac agatttgcgg gacgaatctt cccgtgaagg
     4381 tatgcggatc gtcattgaca tccgtcgtga cgccagcgca tccgttatct tgaataactt
     4441 atataaattg acatccttgc aaaattcatt tggattcaat atgttggcga tcgaaaaagg
     4501 tgtgccgaaa gttttgagct tgaaacaaat ccttgaaaac tatatcgaac atcaacgcga
     4561 agtcatcaca cgacggacgg tctttgaaaa agaaaaagca gaagcaagag cacacatctt
     4621 agaaggtttg cgtattgctt tggatcatat cgatgaaatc attgcgatta tccgcgggtc
     4681 taaatcagat gacgaggcaa aagcaacaat gatcgaacgg ttcgatttat ccgatcgcca
     4741 gtctcaagct attttagata tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat
     4801 cgaaaatgaa taccaagaac tcttaaaact gattgaagat ttaactgaca ttctggcaag
     4861 accagaacgt gttacagaaa tcatcaagaa tgaattagca gacttgaatc aacgatttgg
     4921 tgataaacga cgcacagaat tactagtcgg tgaggtctta agtctggaag atgaagattt
     4981 aatcgaagaa gaagaaatcg ttattacatt gacaaacaat ggttacatca aacgtttggc
     5041 aaataatgaa ttcagagctc aacgaagggg tggacgtggt gtccaaggaa tgggtatcca
     5101 caatgacgac ttcgtcaaaa atctggtttc ttgttctacc catgatacat tgttattctt
     5161 tacgaataac ggaaaagtgt atcgggcaaa aggatacgaa attccagaat acgggcgtac
     5221 agccaaagga atcccaatca tcaatttact aggtatcgat tcgagtgaaa gcatccaggc
     5281 aatcattgcg gtgacgggag aagcagaaga aggccactat ctcttcttta cgacgcgtca
     5341 aggtacagta aaacgtactt ctgtcactgc ttttgctaac atccgaagta atggtctgat
     5401 cgcaattggt ttgaaagaag acgatgagct cgtcaatgtg ctactgacag atggtcaatc
     5461 taatatcatt attggaacac ataacggcta ctctgtcact ttcaaagaag aagcagtccg
     5521 cgatatggga cgtacagctt ccggggtcag agggatacgc ctgcgtgagg aagattatgt
     5581 cgttggggca gccttaatga aggaaaatca agaagtatta gtgatcacag aaaaaggtta
     5641 cggaaaacgg acaaaagtat cggaatatcc agtgaaaggc cgcggaggta aagggatcaa
     5701 aacggcgaac atcactgaaa aaaatggtcc actagctgga ttgaccactg tttctggaga
     5761 tgaagacatc atgctgatta cagataaagg tgtcattatc cgtttcaacg tatcgactgt
     5821 ttctcaaact ggacgttcaa cattaggcgt acgtttgatg aagatggaag ctgatacgaa
     5881 agttgtcacg atggcagcag tcgaaccaga agctgcagaa caaacagttg aagaacaagg
     5941 atcagaagaa taaccatcaa gactaagtgt gtaagctagc aagaataacc gattaaaaaa
     6001 tctggttatt cctgctggct tttttgatta gttgaatttc tcttaaaata ttcgaaactg
     6061 ttcattctgt attgcttttg accaaaatag agtgtataat tagagcttgt gagtagtcta
     6121 attaattaga taactctcct tgctcttagg aacctaagag ccgaaagtcc acagggaggt
     6181 gaatatcaat ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca aacattgatg
     6241 aagaagcaaa aactgcttta atcgaacgtt tcgatgcaat cttgaaagat aacggagcag
     6301 aagttatcga atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc
     6361 gtgaaggtat ctatcacatc gttaaagtat cttctccatc atcagcaaat gctgtcaatg
     6421 aatttgaccg tcttgctaaa atcaacgacg atatcattcg tcacatgatt gttaaagaag
     6481 aagcttaatt gttgtttcat gtgaaacaat agaaaacgat gagaggagat gactattttg
     6541 attaataatg ttgtattagt tggacgtctg acgaaagatc cagatttgcg ttacacagca
     6601 agtggtacgg cagtcgcaac atttactttg gctgtgaatc gtaattttac aaaccaaagc
     6661 ggcaatcgcg aggctgattt tatcaactgt gtgatttggc gcaaatcagc agaaacttta
     6721 gcaaactatg ctcgtaaagg aacactttta ggtgttactg gacgtatcca aactcgttct
     6781 tatgataatc agcaaggaca acgtgtatac gtgacagaag tagtagctga caacttccaa
     6841 ttgctggaaa gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt ccaaagttct
     6901 ggtcaatcag ctggcggatt tggcggaaac aataactcta atcaaacatc tcaatcatcc
     6961 aacggtatgc ctgatttcga ccgtgatact tctgacccat tcggctcaag ttcaacgatc
     7021 gacatttcag acgatgattt accattctaa acgaatttga taggagggaa atagaatggc
     7081 acaacaaaga agaggcggac gtaaacgtcg taaagtagac tacattgctg ctaaccatat
     7141 tgaatatatc gattataaag atatcgaatt actaaaaaga tttatttcag aacgcggtaa
     7201 aatcttgcca cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc
     7261 aatcaaacgc gctcgtatca tgggcttgct acctttcgtt agcgaagact aataaatcct
     7321 aggaattgaa tgactgatcc ttttgatcag ctattcaatt cctttttttc tgtaaaaata
     7381 acctctattc gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat
     7441 atgggaggaa aattatggaa aataaaaaca atcatttatc cccgaaacct ctcttcctta
     7501 tcctgtttat attaatcgaa gtattggtag tcttaatttt accatttcgt tttgttagcg
     7561 cttcggtcat tctattgatg aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt
     7621 ggcttatttt aaataaacgt gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc
     7681 tagcttatgt ctcagaagtc atgcctgtag gagtgattgc ttatcagcca tcaagccaaa
     7741 aaatcgaatg gctcaatcca ttagcattga aaatcaaaga tcaaagtgga ttaacagagg
     7801 aagaattgct agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag
     7861 cagataaaac atttcagttt gatatggata cagtaaaggg tgttgtttat tttaatgata
     7921 ttacccaaaa ccagttgtta aaactccatc agacgaacag tcagccggtg attggtattt
     7981 tatctattga taactatgca gatgccattg ataaattaga tgacaaagag atttcttatt
     8041 tgaactcttt cgtcactaca ctagtttctg actggatgaa tgaataccat gtctttttca
     8101 aaagaatcaa cgcagaacgg tttttcttcg tagcacagat gatcgatctg aaaaagatga
     8161 tggataaaaa atttgatttg ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc
     8221 ctatcaccct tagtatgggg attgcttatg gctcaagaac cctcgatgaa atcggtggag
     8281 aagctcagaa taatttagat gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag
     8341 aggtaaaaga aggagcaaaa cctgtttatt atggggggaa atctgctaag aatgtcaaac
     8401 gcacgcgtgt tcgttctcga gcgatgagta ccgctttgcg tcggattttt gatgaaacag
     8461 gcgatgtgtt tatcatgggg cacaagtttc cggatatgga tgctttaggc tctgcttttg
     8521 gtgtcagttg tctggcacgc ttcaaccaga aaaaagctta catcgttatc aatgagaacg
     8581 agatcattcc agatgtagaa cggtgtcttg aagaaatcca taagcatcct gaattagaaa
     8641 tgcaactaat ctctccagaa cgtgccatag agctgaaaaa agaagaggac ctcctcgtga
     8701 tggtggatta ccataaacct tctctatcga tctctcaggg cctatatgag caatttgata
     8761 agattgtgat cattgatcac catcgtcgtg gggaagagtt tccaagtaaa cctctgctga
     8821 cttatattga atcttcagct tcttcagctt cagagctagt ttctgagctg atccaatacc
     8881 aaagcagcaa aacaaatcgc ttagatcgtt ttgaggccac attattgctt gcagggatgg
     8941 ttgtagatac aaatagtttc aatgtccgta caactgcacg aacttttgac gttgccagct
     9001 atttaaggtc ttgtggagca gacgcatcct tagtacaata tttattgagc tcggatttag
     9061 cgacatactt ggagatcagc aatttgattg ctggaagcga ctatgtgact tctgatattg
     9121 ttgtggcggc agggacagac gaaaaagagt ataatagtgt tatagcagca aaaacagcag
     9181 atacattgct ttcgatgatc aatatcaatg cagctttcgt cattacaaag cggacagacg
     9241 gcttgattgg gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc atcatggaat
     9301 ccttaggtgg cggaggacac ttcacaaacg cagctactca actggaaaat acgacagttg
     9361 aggaaacaaa acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata
     9421 aggagtaatc caaatgaaag taattttttt acaagatgta aaaggaaaag gtaaaaaagg
     9481 ggacatcaaa gaagttccag caggttatgc acaaaattat ctaatcaaaa ataacttggc
     9541 aaaagaagca acgaaaagca gtatcagtca gttagcagga caaaagaaag cacaagaaaa
     9601 acacgaagct gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga
     9661 tactgtagtc gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc
     9721 ttcaaaacaa attgctgaag ggttaaacaa acaatacaaa atcaaattag ataaacgaaa
     9781 aatcgaatta gaaaatccga tccgttcact aggctacaca aacgtacctg ttaagcttca
     9841 tcatgaggtg acggctacga tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc
     9901 gatgttcagc tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa
     9961 aacagctgca gcaatgaaag caaaagctgc aaatcgattt cagattgatt tcggtttctt
    10021 ttttgggaac agcaaaatag cataaagaaa acgagtataa taaaagagaa ctattttttt
    10081 attacacttt cggtacgaat gatgactttt ctctcatttt cttgtaaaat gaagcaattg
    10141 aagacaagga gcgatacgta tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat
    10201 tgaagctgaa caagcggttt taggctctgt ttttcttgac gcagaaacga tcattgatgc
    10261 catggagtac atagagccaa aagactttta tcgtcgtggc catcaaatta tttttgagac
    10321 aatgatcgag ttaaatgatc gtaatgaagc gattgacgtg gttactgtca aagaccggct
    10381 ggaacaagcc aatttgttag aagatgccgg tggtttaagt tatctctcag acttggcatt
    10441 agcagttccg acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt
    10501 gctgcgtaca ttgatccaga cagcaacagg aatcgttacg aaaggattcg agcaaggaga
    10561 agaagttcaa acaattttag atgatgccga acgcagtatc ttggaggttt cggaaaaaag
    10621 aaatcgaagc ggctttttat ctattgctga agtactgaac tccacgattg aaaatatcga
    10681 tcgactagct caaaacaatg aagaaatcac aggtctacca actggctatc aaggtctgga
    10741 taaaatgaca gcaggtttcc agcccgaaga actaatcatt cttgctgcac gtccagcggt
    10801 aggtaaaaca gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc
    10861 tgttgcgata tttagtttgg aaatgggggc agaatctctt gtaaatcgga tgctttgtgc
    10921 agaaggttcg attgaggcca gccatttacg tacaggtcag ctttcggaag aagaatggtc
    10981 gaatctgatc gttgcgatgg ggagtctttc tcgagcaaat atttttatcg atgatacacc
    11041 agggatcaaa atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa
    11101 tctaggcttg atattgatcg attatttgca gttgattgaa ggtaacggta aagagaaccg
    11161 tcaacaagaa gtatcagata tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt
    11221 acctgtcatt gctctttcac agttatctcg tggagtagaa cagcgacaag acaaacgacc
    11281 agtgctaagt gatattcgtg aatccggatc aattgaacaa gatgcggaca tcgtagcctt
    11341 cttgtatcga gatgattatt atgaaagaga aggcggagat gacgatgatg gaaacgagcc
    11401 aaaaaatgac aacatcatcg aagtcatcat cgagaagaac cgaagcggtg cacgaggaac
    11461 agttgaacta ctgtttatca aagaatacaa taagttttcg tcgctttcgc caagagaaga
    11521 attttaaaga agaggttgtg agccttgagc aatcccgaga gctctcgtaa gaagatgcaa
    11581 ggagtaaccg caggaacgac tagagtactt aatttaaaca gatctccaaa gggattgtga
    11641 caaaagtctt gtcacagtcc ctttttccga ataaacggtg ttcaatcagc tgacccttgg
    11701 tattagctca gcaaacgtca aaatatgagg aactattttg acgtttgttt ctctaatact
    11761 caggtcaaaa cgctgatctt acgacctctt ttccttaaac ggtgttctcc tgccaactcc
    11821 tcggtcacaa gtcacaatat catcaaacat gagaagcagt tttttgaaat tgctccttgt
    11881 gagtcagagt t
//
LOCUS       DACELF010000082        11278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.085, whole genome shotgun sequence.
ACCESSION   DACELF010000082 DACELF010000000
VERSION     DACELF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.085"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(40..1419)
                     /locus_tag="IW687_002178"
     CDS             complement(40..1419)
                     /locus_tag="IW687_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ0951429.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IW687_002179"
     CDS             complement(1440..2987)
                     /locus_tag="IW687_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951430.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IW687_002180"
     CDS             complement(3236..4015)
                     /locus_tag="IW687_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0951431.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IW687_002181"
     CDS             complement(4090..4548)
                     /locus_tag="IW687_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0951432.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IW687_002182"
     CDS             complement(4560..5819)
                     /locus_tag="IW687_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ0951433.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IW687_002183"
     CDS             complement(5935..6249)
                     /locus_tag="IW687_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0951434.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IW687_002184"
     CDS             complement(6395..7891)
                     /locus_tag="IW687_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0951435.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IW687_002185"
     CDS             complement(8069..8548)
                     /locus_tag="IW687_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ0951436.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IW687_002186"
     CDS             complement(8924..9169)
                     /locus_tag="IW687_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951437.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IW687_002187"
     CDS             9274..10047
                     /locus_tag="IW687_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951438.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IW687_002188"
     CDS             complement(10276..10524)
                     /locus_tag="IW687_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0951439.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IW687_002189"
     CDS             complement(10623..10937)
                     /locus_tag="IW687_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951440.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            complement(11074..>11278)
                     /locus_tag="IW687_002190"
     CDS             complement(11074..>11278)
                     /locus_tag="IW687_002190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951441.1"
                     /translation="PTQNIIEPNKNGEELYEYAMILNNPEYIESGKAETVNYYYELPS
                     EIQNYQIVIREADGYFVGLPFTL"
BASE COUNT         3504 a         2318 c         1690 g         3766 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt atttggttct ataatatttt gtgttggt
//
LOCUS       DACELF010000083        10851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.087, whole genome shotgun sequence.
ACCESSION   DACELF010000083 DACELF010000000
VERSION     DACELF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.087"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..770)
                     /locus_tag="IW687_002191"
     CDS             complement(54..770)
                     /locus_tag="IW687_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0951442.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(820..1029)
                     /locus_tag="IW687_002192"
     CDS             complement(820..1029)
                     /locus_tag="IW687_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951443.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1306..2579
                     /locus_tag="IW687_002193"
                     /pseudo
     CDS             1306..2579
                     /locus_tag="IW687_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2686..3717)
                     /locus_tag="IW687_002194"
     CDS             complement(2686..3717)
                     /locus_tag="IW687_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951444.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3724..4554)
                     /locus_tag="IW687_002195"
     CDS             complement(3724..4554)
                     /locus_tag="IW687_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951445.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4551..5357)
                     /locus_tag="IW687_002196"
     CDS             complement(4551..5357)
                     /locus_tag="IW687_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0951446.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5363..6187)
                     /locus_tag="IW687_002197"
     CDS             complement(5363..6187)
                     /locus_tag="IW687_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0951447.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6177..7277)
                     /locus_tag="IW687_002198"
     CDS             complement(6177..7277)
                     /locus_tag="IW687_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0951448.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7455..8240
                     /locus_tag="IW687_002199"
     CDS             7455..8240
                     /locus_tag="IW687_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0951449.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8273..8656
                     /locus_tag="IW687_002200"
     CDS             8273..8656
                     /locus_tag="IW687_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0951450.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8732..8863
                     /locus_tag="IW687_002201"
     CDS             8732..8863
                     /locus_tag="IW687_002201"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0951451.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            8832..9068
                     /locus_tag="IW687_002202"
     CDS             8832..9068
                     /locus_tag="IW687_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0951452.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9146..10117)
                     /locus_tag="IW687_002203"
     CDS             complement(9146..10117)
                     /locus_tag="IW687_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0951453.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3225 a         2516 c         2071 g         3039 t
ORIGIN      
        1 cgtaatctga aaatcagaag aagtaccgtc ggctcaagcg atattggaaa ctcttactca
       61 aagattccac gacattggag ccgctcaaac ggcattatca tcgactgttt aaacgaccaa
      121 ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg
      181 acaccttcca tatccagagc agtcgccaca tcacggggat ggatgccatc caaattaaaa
      241 gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt aaatggtgat accgccgatc
      301 gcttgtaatt taggtaaaac gtaggcaacc agttcttgct cataacgatg aatattctcc
      361 ataccaatct cattcaagta atcgattgct cctgcaatgt tcggagtacc agcttcaaat
      421 ttccatggca gctctttcca tgaactatcg taaagattga caaaatctat catttcaccg
      481 ccgaattcga ctggctccat ttgttctaac aattgacgtt tgccatataa tacaccgatt
      541 cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt
      601 acatcaactg tcatatgcgg tgctgcttgg gcaccgactg gactatcctc aactacaaca
      661 gcctgttcaa ccctcagccg tttcaagcac aacaaataac cttctggatc tggtttgccg
      721 acctgaagat catcgatcgt cacaaaggaa tcaatttttt ccttaatcat cggatgtgct
      781 tcaaaaaaat agtctagctc ccgcagttca gaattgagac tactccgatt ttccagtgct
      841 gctgatccgc tgctcaaaat gttcagcatc cactggtctc ccaaccgctt gacttatccc
      901 ttcataaaag gacagttctg ctgccgtccc aacaccttgt tgatagtctg caaaaggaat
      961 tttatagccg aactgctcca gtaaataggc ttcagtaaat agtgatgaat actctcgtaa
     1021 aacatccatt cactgtcaat catcgtccca tcaaaatcaa aaataagtgc tttggtcatc
     1081 ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct gctctctcta cagtatcagc
     1141 ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg tggcgctata atttctagga
     1201 ctgatagtaa atcattctat cagtcctttt gagcataaaa aaacaaaaca tacagaagct
     1261 ccgttagaat tgaatcacca caacacaatc aaaggagcgt tctgtatgcc tgatcttcat
     1321 cttatcaaac tattactagg aatcaaagat ggaaacattc aaatcactca tgtcgagaag
     1381 aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca cgctcattaa aacgattcag
     1441 ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga tcgttaagaa cggaaagaaa
     1501 aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga cttatcttgc tttggcaaag
     1561 caacggtacc tctgtcgtgg ctgtcacacc tattttacgg cgaataccta cattgttgaa
     1621 aaaaactgtt ttatcgccaa acaagtacat tataaaatat tggaggaatt gactgaaaaa
     1681 caagcaatga cgacgatttc taaacattgc ggcgtttctt ggtcaaccgt ttcgcgaaca
     1741 ctcgcttctc tgctccccat gacaaacgtg aaaataaatt ggttgcctcg ctgttcattg
     1801 gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct attctttttc ttgtatggat
     1861 ggcgatactg ggaaattact ggatattctt ccttcacgta agaagaaaga tctcatttct
     1921 tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa aaatactcgt aacagatatg
     1981 aacgcctctt atgctttgct gataaaagag tgctttcctc atgccaaact cgttgtggat
     2041 cgctttcaca tcgtcaagca cctcattcgc agttttaagg atatttgact atgagtcatg
     2101 aaaagcttcg accgaaacga tccaatacaa gccaaacact atcgacaagt gaaggcttta
     2161 tctcgacttc tgatcaaacg acaggacacg cttgtttatg ataaatggac aaaatggcgt
     2221 aattttggtt gggcctattt aaccgaaagt gaagtcgtag aacgtctact ttctacttct
     2281 gatgaactac gaatagctta tgcttactat caagaaattc ttcaagcttt ttatgacaaa
     2341 gaagcggaca tcttctttca gcttgtaaaa acaatgccca agtcggttcc aagagaactg
     2401 catcatataa aaaaagcgtt tataaactat gaatccggca ttcgtttagc cttagagttg
     2461 ccttactcaa atggcaaaat agaaaatctg catacccaca tcaaagcatt gaaacgaata
     2521 gcttacgggt taggatgaga accagaattt tcttaatcaa tcatctgatc acttattaaa
     2581 cataaaaaaa catctaaagc agaaaatcaa ttctacctta gatgttctta tatcactcta
     2641 tcagtcctat ttgacaaaga gccagaaatg tttgccgact atacactagc tttgttgtgc
     2701 aaaatgctga gtatcaattt ccacagggat tgctagaata tctttcagct gaatgtataa
     2761 ggcatcgcta attttctctt cccgatcgtt caaccgatcc actaaaattt ctttgccctc
     2821 cagcgtttga aaaacatacc gttgaatgtt gcctaaaata aatgattcct tccatagaac
     2881 cggaatctta accccctgag gaaccggttc aaattgaatc agctccggtc ttaaataata
     2941 aatgcagcga ttgcccttag cggaaaactc gtgggtatac tgattcagct cataccattc
     3001 gaaacgatta tagttgccga taaattccgc aacaaatcgg ctctgaggat tctgatacaa
     3061 ttccttcgag gtggaaacct gagccacccg cccagcttcc atcacaaaaa tccgatcact
     3121 gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc atcgtcatcc ctagcgattg
     3181 ctggatagcg cgcaaatccc gctgcagctg ttttctaatt cgggcatcca aagcacttaa
     3241 tggctcatcc agcagcagga ctttcggttc agtgaccaat gagcgagcca gtgccacccg
     3301 ttgtcgttga ccgccagaaa gaaactggac atttttatct gcctgctccg tcatatccac
     3361 caatgatagc atctcaaaaa ccttttcctg aatcacctca ggactcagct tctttacctt
     3421 caaaccaaag gctacatttt caaaaacagt catagttgga aacaatgcat actgttgaaa
     3481 aacaaagccg atattgcgat ccttcgtagg cttatcggca atattctcat tgtccagcac
     3541 aatctcaccg ctctttggtt gttccaatcc cgtgatacag cgcagcaacg tagactttcc
     3601 gcacccgctg ggacctagta aagtaaccag ctcacccttt tcaatctgaa tttcatcaat
     3661 agctaaaatc tgtttttcat caaaaaacag attgagatca tgtatttcta aaaacatttt
     3721 ccgtcacaac tcctttaagg gcttcttttt cgactgcttc tcttccctcg tctgcttaag
     3781 ggacagctgt tgcttttgtc gataagtcag cataaaggta gttaaagcaa ttcccaccat
     3841 aaacagcaaa tacaaaacca tcacagcgct agccaaatgg ccgttttcgt tcatccgccg
     3901 cagcatatag atgcgtaccg tctgaaatcg accgccaatc aacaggttcg tcagcatgta
     3961 ctccccaaaa accgaagaaa aaatcatcag agaaactagg gtcacaccga tacggatgtt
     4021 tggaaacagc acctggataa taatcgtaga catcggggcg cccaaggttt ctgctgcatc
     4081 cactaattcc ttcagattaa ttaagcgcat ctggttgtta agactaagat agacaatcgg
     4141 caacgccgta ataaacaatg cacctaccaa cactacaaac attgggatac cagtttcagc
     4201 atacgttcga agcaaagccg ttaccaaaat cactcctggt aaagcatacg gcagcaggag
     4261 cagcttttcc agccaacgat tcaaacgagg gaaatacaaa tgaatccaaa taatggtggg
     4321 aatcatcaac aacagaatcg tcaccaagac cacacctgct aataaaagtg agcgacccac
     4381 cgcagcaata aattccctgt ccgtcagcag ctgttggaac cattgcaagg taaaatcact
     4441 aggtaaaagc gattttgacc atttagtgga gctggcatac aaaatcgttc caataatcgg
     4501 cagcaagtaa tacaggacaa agcagaacaa aatcggtttt attaactttt tcacgtctgc
     4561 accaccttgc ctttcttttg aataatcaac attgccgtta ccagcaggac acctaaagcg
     4621 actgccaacg cactgcctaa attaggcttg gcgaacacat ctcccgacac taaagccgca
     4681 attcgaatcg ttaaaagatt tacattactg ctggtcaggg catacgctgt ttcataggtc
     4741 cccatgccat tagcaaataa gatgacaaag gtgctggcaa tcgatggtcg caaaaaaggc
     4801 aaagccacct tgaaccaagc atacgctgat gaagcgccca gcaattgtgc ggactccagc
     4861 cattcctttt tcacttcctt catgctggga tacaaaaaca agacccctaa cggaatttgg
     4921 aaataggtat acgtgatggt caatccccac caagaataca aattgaagtc cgctagaaat
     4981 ggcaaaatca ttttcagcac acctacattc cccaacaaaa taataaaaga gaatgccagc
     5041 ggaatcccag caaaattagc tgccaaattg gaaacaaagg tgattttctc ctgcaattta
     5101 gtcgatagat tcattaagca taaacataaa ataaacgtcc cgattaaccc aatcacactg
     5161 gagctgacag caatcagtac gctgttaaca aaggcctgtg tgtaatattt gttgctgaaa
     5221 atatcactgt aattggccaa tgtccactgg ttagttaaat cactgtgcaa gctgcacaga
     5281 accataaaaa gcagcggcag cagtaaaaat gcacagaaga taaaggaggc aggaaccaga
     5341 accaagcgtt ttttcatctc tttcatcccc tcatcaattc ttgaatgtcc tgcattttct
     5401 ctgacggtgc aattcctaat aaccagcaaa acaatggagc cgccagcagc tgtggtacag
     5461 tttgttcctt cagcttttgg cgtttatttg aaatcaaata aaaaggaact tcacgatgtg
     5521 cagccaagct gccgccatgc agtccgtgcg catccatccc gtgatccgca gtcaccacga
     5581 cttgataacc tttttgcagc cattcaggaa ggtaaatagc caataatctg tccgcctgat
     5641 taaccgctgc tgcatattca tgagaatcgg cggtaaattt gtgaccgaca tcatcaatat
     5701 tcatcgaatg tacgaccaaa aagtctggct gatactgatt cactaaataa tgggcatccg
     5761 caaataaatg cgaatcaggg taagtgtcct cactataaaa gatgccatgc tgaatcagtc
     5821 gctcctcctg atgctggatg cgatcaacca aacgattata gggggcttga ttgtacaact
     5881 cgctgtacca gtggtaagcg gcggctcctg tttttccgcc agcttcctta actaattcaa
     5941 acagcgacca ctctttggat cgctgagcat ggtaattact atagatgcca ttttcatagg
     6001 ctggaacgcc ggtcatcagt acttcgtata acggacgtga attgctgggc agctcacttt
     6061 ttaccttcaa taagcttgct tgctggtgct caactaaatg attcatgaac ccaagctggg
     6121 cgacggctgt gtcgtagcga cagccgtcta gtataactac aatcagtttg tcaagtctac
     6181 tttgcatagg tcagtacctc cgcctgccaa agctctggaa gctctacaga ggtcttgttc
     6241 caagcttcat tgtcttcaac tggcaccgcc gcttcataag catcctccgg cagtaatttt
     6301 tcagctactt ctttaggcag ctctaccttg cgaataggtc gcgcatagcc ctttgccaaa
     6361 ttgatttgcc cttcatcaga taaaatatat tcccgtgcta gctttgccgc attgggatga
     6421 ggggcatttt tattgattaa ggttgtatac ccgctaatga tggagccgtc tgttggcaca
     6481 gttacctcaa aacgctcgga gtcaatttgg tcacgataat ttagtccatt gaagtcccaa
     6541 acaactgcca catcgatttc acctttttca agatttgcta cgctggcatc aacggtggac
     6601 aaacggcctt tttctgccat ttttttgtag taattcagac ctggctgaat atccttttcg
     6661 tcacctccgt tggcaatcgc cgcagccaat acagcaaatt gtgcttggtt ggcaatcgtc
     6721 acatccccca cgctaacttg atagtcaccc tcttgcagct ccttccagct agtcggtgct
     6781 tgcttcacat tctctttatc ggtgataaag gaaatggttc ccgtatagct taacaaccaa
     6841 tccccatcat catcctttgc ccaatccgga atttcctccc aatagttggt cttataaggt
     6901 aaggttaact ctttgctttc agccaacgga ccgaagctga taccgacatc accaatatcg
     6961 gcggttccat tttttccttc tgattcaaac ttagccaatt cttctgcact ggacatatcc
     7021 gtatccacgt gacttaagcc gtattctttt tcaaggtcgt tccaagtgcc tacccaattt
     7081 gcccaagtat ccggcattcc caagctggca atctctcctt cttccttggc tttttctata
     7141 atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg ttgcttcacc tgagacgcag
     7201 cctgtcagca ggatacttac tgttgctgca caaaataaac cgatagtcat gttacgaatt
     7261 ttttcattca ttttcattca ttcttctccc ctttcgataa ttccattcta acaaagcttt
     7321 gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca atgtaaaggt tgtatatggt
     7381 ttgtaaattt cgacgaattc ccgttaacct tctaaagctt ttgcggtatg atagtagaaa
     7441 aaaggaggct ccctatgcag tattatgatc agcatctcca cacgtatttt tcctttgatt
     7501 cagaggaaaa atttgaaaac tatttagcct atcagccgga gtttttcgtt tctacggatc
     7561 actttgattt aaacaatcca tctaccggaa aagatgacat tccggattat ccagcctata
     7621 taaacaaact ggactcttta aaagaaacct attccaccaa atttctgcga ggtatcgaga
     7681 ttggcgttgt tccaggacaa gaagagcgga tcgtggatta cctcgctcag catccttatg
     7741 atttaaagct ggtaagtatt catcaaaatg gccgctttga ttatatggac gatgtggttt
     7801 taaccaaaga tcccattcaa actaccaagg aatattttga tcagatgttg caggtcttga
     7861 cggattttcc agaaggagat attttgactc actttgatta tggattacgc cgcttcacct
     7921 tcacagcgga agaattcgcg gagcattttg aggctcagct gctaacgatt ttcaaaaagg
     7981 ttgttgaacg agaattggcg atggagttaa atgccaagag cttcttgaaa tacggcaacg
     8041 aagaccttta ccgttatgcc gttcctttgt atcaaagtgt cggcggaaag ctctttacct
     8101 taggctcgga tgctcatgtt gccgaagatt atcagctggg ctttaaaaaa atgagtcagc
     8161 tgttggcaga ctgtcaggta gaaaagttgc tagtcattca aggaaaagaa cgccgcctga
     8221 cgccgctgcc gattttgtga ttcctatata atagaagaaa gtggtgaaaa aaatgagcaa
     8281 cctgtgggac tacaatcaag aggcacccat ccattattta attgcgcgac attgggatgc
     8341 gttgaaaatt gaggcggttt gccgatcctt gttagcagcc gttccaaaac aacaactgga
     8401 aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt caggcttatt ttgcagcctt
     8461 taaagatcag cctttggaat atttgcatgc gcagtttcat cttttttatc aagtagccgc
     8521 cccagatgac tacaacgatt tacgaggaca gctgcaattg acctttcaag cggatgaaac
     8581 cgcctatacc gttttattgg gaatggctcg ccttggtgat caggcgaagg tcgagtggcg
     8641 gattttcgat atttgatggc aggatcaagg cgtttcagtt gcaaaccttc ctctctttca
     8701 attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc accgatgctg gtgaaattcg
     8761 tctccgctta tttcctgacc aagctcccct ggctgtagcc aactggcgct ccctagctga
     8821 gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat taaggaattt gttattcaag
     8881 gtggcgcttt ggatggttct ggtgatgaag ctgtttccag ctatgagggc tttttcgctg
     8941 atgaagtgca tcgcggactt tatcatttta atggcgcctt ggccttaggg gaccacggcc
     9001 cccacaccaa cggcaaccag ttttttattg tgcaaaacac aaaggcgcag gctgacctgc
     9061 taatgtaaag caagcatact tggagcttgg aggactaccg gagctggatg gtcgttatac
     9121 tgtttttgga caggtatacc aagggttaga aatcgtagca gcgatcgcag ctcagccgac
     9181 aaacagtgaa gatgtaccga ttgagccagt gttcattcga caaattaact tcgaaaaaac
     9241 atcgtaaaaa gacactcact gacttcagca aaagctctat tcttgagctg gctgcagcta
     9301 gtgagtgtct ttcatttatc cataagataa agcttggatt caataaataa ataaatgttt
     9361 tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa
     9421 acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg atactccagt cccaatgctt
     9481 ggtaaccgtg cataccatag cagtggtacg gtagtaattc taacggtacg aaccgatcaa
     9541 gatctgccac aaaacaagca atcgtcagca gctcggtcag gttcatattg atacctggaa
     9601 ccacaggaac tcgaattcgc acttcgcagt taggatactc aattaaaaag cgacgaatat
     9661 tcttcaaaat ttgttggtta tctaagccgg tccattgttt gtggagcttg ctggtaaaca
     9721 tttttacatc cacaaacaat gtatccacat agggagctac cttttggatt tcattataag
     9781 caccacaaaa gctggtttcc agtacggtat tgattccaat gtatttcgat ttttgcaaaa
     9841 tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc gctaatggtc actccgccat
     9901 ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc cattacctct tcagcggtca
     9961 tggtctggcc aaatcctttt tcaatctgct tgctttgtga ttcgggaatc gaacaccagg
    10021 cacagcgtaa tgggcagccc tttaaataaa caacggtccg taaaccagca ccatcatgta
    10081 gagaaccctg ttctattctt aatactgttc cttccatata gatttttgaa ttacacacta
    10141 gtttaaacta aaacacacaa ggaaaaactt accatttaat cataaaatta gctcatataa
    10201 atatactagg atgtaaaata gactggtcaa aatctggtca aaaaagagga ctaatacagg
    10261 tagtcctctt ttttgttatt ttaatattct aaatcatttt ttattggttt attcccttat
    10321 tcgatgtaga ctcaaatcac tatatcacta gatatcaaac ggcgtcatac ggaatcaaaa
    10381 acatcaaatt tgacctgtta aatggtcatt gaataacatt caaaatcaat aaaacggagt
    10441 cagacacgga gtcaaaaacg ctgagagtaa aaaatatata cagccttgta ggtaaagtac
    10501 cacagggctg tatatttttt atttcagatt accttaaatg attccattta ctttttatct
    10561 cattttttcc tactagttag tatcaaatac tctcctattt tctcaataaa ctatttttgg
    10621 atacatggta tctgaaacag ctaaaacctt gtcattacag caaaaaatca cgttatccag
    10681 actgtagaca aagtccattt tatggatgat gtctacagtc ttttctttta taatagattt
    10741 atgaaaacga atgcaaggag tgatagctat gatgtctaaa aacaatacga atggtagaaa
    10801 tcaatttgcg atgcttacga ttgatgatct agtttctcaa gatcatctcg t
//
LOCUS       DACELF010000084        10767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.088, whole genome shotgun sequence.
ACCESSION   DACELF010000084 DACELF010000000
VERSION     DACELF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.088"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW687_002204"
     CDS             79..1125
                     /locus_tag="IW687_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0951454.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW687_002205"
     CDS             1474..2106
                     /locus_tag="IW687_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0951455.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IW687_002206"
     CDS             2694..4133
                     /locus_tag="IW687_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0951456.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IW687_002207"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IW687_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IW687_002208"
     CDS             4895..5110
                     /locus_tag="IW687_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951457.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IW687_002209"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IW687_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IW687_002210"
     CDS             complement(5770..6681)
                     /locus_tag="IW687_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0951458.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IW687_002211"
     CDS             complement(6700..7704)
                     /locus_tag="IW687_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0951459.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IW687_002212"
     CDS             complement(7701..9824)
                     /locus_tag="IW687_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0951460.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IW687_002213"
     CDS             complement(9829..10749)
                     /locus_tag="IW687_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0951461.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACELF010000085        10767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.089, whole genome shotgun sequence.
ACCESSION   DACELF010000085 DACELF010000000
VERSION     DACELF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.089"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            314..2014
                     /locus_tag="IW687_002214"
     CDS             314..2014
                     /locus_tag="IW687_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951462.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(2116..2949)
                     /locus_tag="IW687_002215"
     CDS             complement(2116..2949)
                     /locus_tag="IW687_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0951463.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3253..5418)
                     /locus_tag="IW687_002216"
     CDS             complement(3253..5418)
                     /locus_tag="IW687_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951464.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            5678..7972
                     /locus_tag="IW687_002217"
     CDS             5678..7972
                     /locus_tag="IW687_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0951465.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            7965..8630
                     /gene="pgmB"
                     /locus_tag="IW687_002218"
     CDS             7965..8630
                     /gene="pgmB"
                     /locus_tag="IW687_002218"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0951466.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            8623..9630
                     /locus_tag="IW687_002219"
     CDS             8623..9630
                     /locus_tag="IW687_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0951467.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            9652..10671
                     /locus_tag="IW687_002220"
     CDS             9652..10671
                     /locus_tag="IW687_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0951468.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         3261 a         2283 c         1956 g         3267 t
ORIGIN      
        1 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
       61 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
      121 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
      181 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
      241 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
      301 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
      361 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
      421 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
      481 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
      541 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
      601 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
      661 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
      721 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
      781 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
      841 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
      901 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
      961 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
     1021 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
     1081 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
     1141 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
     1201 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
     1261 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
     1321 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
     1381 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
     1441 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
     1501 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
     1561 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
     1621 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
     1681 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
     1741 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
     1801 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
     1861 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
     1921 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
     1981 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
     2041 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
     2101 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
     2161 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
     2221 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
     2281 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
     2341 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
     2401 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
     2461 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
     2521 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
     2581 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
     2641 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
     2701 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
     2761 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
     2821 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
     2881 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
     2941 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
     3001 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
     3061 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
     3121 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
     3181 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
     3241 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
     3301 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
     3361 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
     3421 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
     3481 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
     3541 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
     3601 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
     3661 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
     3721 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
     3781 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
     3841 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
     3901 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
     3961 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
     4021 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
     4081 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat acgtaacaac
     4141 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
     4201 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
     4261 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
     4321 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
     4381 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
     4441 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
     4501 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
     4561 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
     4621 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
     4681 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
     4741 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
     4801 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
     4861 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
     4921 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
     4981 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
     5041 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
     5101 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
     5161 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
     5221 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
     5281 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
     5341 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
     5401 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
     5461 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
     5521 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
     5581 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
     5641 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
     5701 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
     5761 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
     5821 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
     5881 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
     5941 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
     6001 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
     6061 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
     6121 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
     6181 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
     6241 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
     6301 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
     6361 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
     6421 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
     6481 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
     6541 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
     6601 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
     6661 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
     6721 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
     6781 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
     6841 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
     6901 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
     6961 ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg gcgatacgga
     7021 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
     7081 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
     7141 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
     7201 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
     7261 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
     7321 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
     7381 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
     7441 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
     7501 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
     7561 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
     7621 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
     7681 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
     7741 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
     7801 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
     7861 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
     7921 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
     7981 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
     8041 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
     8101 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
     8161 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
     8221 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
     8281 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
     8341 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
     8401 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
     8461 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
     8521 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
     8581 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
     8641 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
     8701 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
     8761 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
     8821 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
     8881 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
     8941 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
     9001 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
     9061 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
     9121 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
     9181 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
     9241 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
     9301 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
     9361 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
     9421 acttctaata aaagtatggt attattttct acgactggat tcgaagctga tttctctgtc
     9481 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
     9541 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
     9601 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
     9661 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
     9721 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
     9781 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
     9841 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
     9901 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
     9961 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    10021 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    10081 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    10141 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    10201 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    10261 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    10321 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    10381 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    10441 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    10501 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    10561 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    10621 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    10681 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    10741 ttctaacaca ttgtttatct gtaaaaa
//
LOCUS       DACELF010000086        10369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.090, whole genome shotgun sequence.
ACCESSION   DACELF010000086 DACELF010000000
VERSION     DACELF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.090"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..168
                     /locus_tag="IW687_002221"
     CDS             <1..168
                     /locus_tag="IW687_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0951469.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVAPGDAQRLSENLKIMQ
                     KELQKIWQQLK"
     gene            150..1064
                     /locus_tag="IW687_002222"
     CDS             150..1064
                     /locus_tag="IW687_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0951470.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1304..1993
                     /locus_tag="IW687_002223"
     CDS             1304..1993
                     /locus_tag="IW687_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951471.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKV
                     EEACSELFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2113..2199)
                     /locus_tag="IW687_002224"
     CDS             complement(2113..2199)
                     /locus_tag="IW687_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0951472.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            2845..3777
                     /locus_tag="IW687_002225"
     CDS             2845..3777
                     /locus_tag="IW687_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0951473.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            3794..4264
                     /locus_tag="IW687_002226"
     CDS             3794..4264
                     /locus_tag="IW687_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0951474.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            4348..4665
                     /locus_tag="IW687_002227"
     CDS             4348..4665
                     /locus_tag="IW687_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951475.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            4955..6136
                     /locus_tag="IW687_002228"
     CDS             4955..6136
                     /locus_tag="IW687_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0951476.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            complement(6365..7513)
                     /gene="tnpB"
                     /locus_tag="IW687_002229"
     CDS             complement(6365..7513)
                     /gene="tnpB"
                     /locus_tag="IW687_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0951477.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(7530..7934)
                     /locus_tag="IW687_002230"
     CDS             complement(7530..7934)
                     /locus_tag="IW687_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0951478.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            8563..9717
                     /locus_tag="IW687_002231"
     CDS             8563..9717
                     /locus_tag="IW687_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0951479.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            9711..10133
                     /locus_tag="IW687_002232"
     CDS             9711..10133
                     /locus_tag="IW687_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951480.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT         3732 a         1699 c         1679 g         3259 t
ORIGIN      
        1 caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgccaagtgg
       61 tgcaatacac accaacatga cgttgctcct ggagacgccc aacgtttatc cgaaaaccta
      121 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagt cgaaccactt
      181 cctgtagccg agcaatcaat tacgcagaac cacgagcaac cattaaaaca ggaataaact
      241 gtgacgtcga ttatgccaaa agtgaaatga aacaaattcg catgctgtat ggaaaagacg
      301 accgtgtaca ggcacactta ttgattcaat cttttcgacc aggagaaatt accgctgaaa
      361 aagctaatca actaggggca gagtatgcaa aaaaaatagc ccccgatcac caaacagcta
      421 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
      481 tggagactgg aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa tgttatgacc
      541 taaatgacga gatttgtctt gcacataact taccaatcac tgagcgagga aagaaaccag
      601 aaaaacgaac catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt tggaaagacg
      661 aaattcggat tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc tatgaccaat
      721 tttgtaattc cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa aatttcactt
      781 acgaattgat ccacaaaaag aaaaaagtac gatccaacag actaggaaag gactacgaaa
      841 aggagacgat tttacatgag ctggacagac gagaacgaac ctataaccaa cagcgaacca
      901 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
      961 aacgaactct tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac catgaacaac
     1021 acgttgaaag aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacata
     1081 gaaaacgaaa taaagaaaca acgtgctttt ctcgttcctt atacgcaaga aaggatacaa
     1141 actacgattg atttacaagt taaaaaactc catcaagagc tattagacca gcgaaataag
     1201 gaacagaaaa aggtgcttat tcaacgatca ttggattttc ttgccccctt atgttgctta
     1261 gtcctgacac tgttcgcatt ttggttagta tggggaggct aaaatggcat tttatttacc
     1321 ctatctactc atttttgtta gtatttcagg aagtatttgg ctcatctata aaatttttca
     1381 aacaagatat tcattaaaag gctctaaaat tcgatttaag cgatttttct ttcttggctg
     1441 tatattttcc ttgataatcg tttcaagcgg cttattcggc gttttagagg gaaataagcg
     1501 cgtttctaga agtattttat taggaaatgt cactcaaaag tatgaatcag cgcgtgataa
     1561 aaagaaaaaa gagcaagcat tagcacaaaa aatggaagag tttaccactt gttatgagga
     1621 aatgaacgat atttttgtga atcaagaaaa acgtttgacg gataaaaaca tggaaaaact
     1681 cactcgtctt tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag acaactatga
     1741 gcgaaccaaa aaagacgtgc aatatgtgaa agatacgaaa gtggaagaag cttgtagtga
     1801 gttatttgga gatacaattc ctttttcaac tagcgaacaa gaacgaaaag aacgacaaca
     1861 atcagtcaca tatgaacgct atgaaatctt gcttcaacaa gcaattaaca tacaaaatct
     1921 gactaaaaaa gaaacagctt taaattattt aaaaagcgtc aaggaatggt tagatcagca
     1981 acagcaaaat tgaatttgat tgaaattcat gttaaactaa acatgcaaat gttcaaacat
     2041 tagatacacg aaagccccag agaggtcgga ttctctgggg ctttttttag gttgatgtcg
     2101 cctttactga ttttacttct tgtttcggtt attcaaccaa taactgaata cagagataca
     2161 aactcctaca agaagggggc aaataaacag ttcaaacatc agatacacct ccttccagtc
     2221 gcaagactgt cggataggcg acaagttatt ataccctaca aaattaaatc tgtgtggcaa
     2281 ttttaagtat ctcttatttc cgggttgtta gggcttgccc tgaccgcctg tagggcgctt
     2341 taaattccag ataaagtttt ttatactaag actgtcttaa aacggcttag acaagctctt
     2401 agagcctcaa ccctcctttt tgaacaatgg ttttagcaaa ggagctggag gcgactgcgc
     2461 aggtataatg tgagagcgta gcgatcacac ctttttggcg accgtaggga gcgaggtttt
     2521 gaattagcag tttagaaaag ttcgcgaacc tgtttaaaga aaaaataatt gtttttttag
     2581 gtgggaaatt ttgtctccct cttttataat ctttttttaa acctcttttt aaacctcttt
     2641 aacgtcggct gtgtccttac tcccccaagg tatacaaaat tttataggca tgaaattatc
     2701 tgtttatagg catgaaatta tctgtttata ggcatgaaat tatctgttta taggcatgaa
     2761 attatctgtt ttaggtattg tttttagaca taatatgtcc taaaatgatg tcaataaaat
     2821 catttagact ggagtaacaa ctaaatggca aatgaaatcg taaagtatga accagaatta
     2881 aataccattc ctttaagaaa atttacccca atagaaatga acttgttttt ttctattgtc
     2941 tctagaatgc gcgataaagg taatcaaaaa atacgctttt atttcagcca acttaaagat
     3001 ttaagtgcgt ataagcagac tgcaggagaa gtctttataa aagatttggc aagaacatac
     3061 gatcatttga tggatttacg tttcggaaga aaaagcaatg gtggattaac aatagaacga
     3121 tttacaatgt ttactgattt tgaaattcat ggagaagtcg ataaccctta tgtagatata
     3181 aaagtccacg aaaaagcttt gcctttactt aataatctag aaagctgggt acgtttctct
     3241 ttaacagaat ttcgagattt aaatagcagt tatgctaaaa caatgtttag attgctaaaa
     3301 caataccgaa ctcaaggatg ggctgaattt tctaaggaag attttttaga gctattagat
     3361 attcctaaaa gctatagaca aacaaatata aatcagtttg tactcaaacc aataaaagaa
     3421 gaactaactc cactatttaa aggattaact attcgtaaaa aatatggtaa aggtcgaggt
     3481 aaacctgtta ttggttatcg ttttacttgg aaagctgaaa tcaaccatgc tgacgatttt
     3541 tcaaaaggga agcaagaaga tttacgtata aaattattta atattgaaca caatggagaa
     3601 ttaacacaaa aagaaaaatg gcgtgctaaa gatcgaatac taaatcttcc gttaggtact
     3661 catgaagctg attttaaaaa acaacaacaa accgaaaaag aagaagcgga aaaacaatca
     3721 atctcaaatg agcttaaaca agatttatta gaaaacttac aaaacttatt cgattgaagc
     3781 ataaggagta tttatgatag ataaaacgat caaagaactc gcggaagaat ttcatgtttc
     3841 aaaacaagca attaggaaaa ggctatcagc tgattttaga gaaaactatg tgcaaactgt
     3901 tactaggaac ggagtgaaaa ccttagttgt cagctacaaa ggatatttgc ttttaaaaca
     3961 acatttttta ggtagtaaca ccactggtaa ccaagtagaa accgttgcta gtaacgatga
     4021 aaactcgatt attgtttttt tacatgaaca gctccaagaa aaagatactc aaataaaaca
     4081 aatgcaaaag ttactcgacc agcaacaaca actcaccttg caggcaaatc aacagatcga
     4141 aaaactgcaa gaacagttac aattagctta tacggaagaa cctgaagaag aggaattaac
     4201 ttctgtcaat aaagaaaaca acaatgcaat aacaacagaa aaaaagtggt ggaaattttg
     4261 gtaacaatta ccaactttcc ataaagaaga aatcaatttc tatagactct atctttctaa
     4321 gaataaaaaa tatgtttagg tgatcaaatg actataagta aagtaatcaa acaaaaaaga
     4381 atagaaaaac aactaactca agaagattta gctgaaatgc ttttagtatc taaaaaaacc
     4441 atttctaatt gggaaaatgg tcgaacaata ccagatacag aaaatttaat tcagtttgct
     4501 aagttatttg atttatcact agacaatatt ttgttaggag aagaaaaaat tatggtaaaa
     4561 aattatggta aaaaatcata caaaaacact aatactatta tttcattttg taattttttt
     4621 aagtttagtt ttcaccttaa ttcagtccgg attcttttat tttagccatt caattgccac
     4681 ttggttatta atattttttc taagtttact attaataatt aatacattaa taattaatac
     4741 tttaattatt atctttaata aacatagaaa cttatttctt cacagcatat ttccaattgt
     4801 tttattaatt tttctatact ttatataaaa agatagaact atttagtaag ataaaaaagt
     4861 agctaattgt aattttcgtc taaaactcaa aactagtaaa aataacttta ttgtgttatt
     4921 tttatttata aattatgaaa aaggaccttt cgaaatgaaa aataatagaa tctacaaaaa
     4981 tcagttgaat tatgggcttt tattgccagt ttttctgcta tgtatcattg gattactttc
     5041 tttatatgtt gctttatatc acgactctta cactaattca aaaatcacaa aagaattaat
     5101 taaacaagta ttgtggtata gtattgggtt cacttcagca ataactattt atttttttaa
     5161 tcctaaaatt ttgtggaaat taactcctat attttacgga ttatgcatca taatgttatt
     5221 acttctcttg aaattctata atccacaatt agcagtctta actggagcaa aaaattggtt
     5281 ttatattgct ggtttttcgt ttcaacctac tgagttagtc aaaataggat acacccttat
     5341 gctagctaaa ataaccgtca attatcagca aaaatctatt actagtaata cgaaagatga
     5401 ttggatttat ttacttaaaa cttttattgc tactcttcca ataggaatat tattattttt
     5461 tcagaacgat ctaggaacaa tgttagtctt cattgttatt tatattttta ttttgtttac
     5521 gtctaacata aatataaaaa taattgcccc aataatcgcc attggtgttg ttttatcctt
     5581 aatattttta tatttggtta tatcagatac aggaagagat ttcttattaa aaataggatt
     5641 tcacgactat cagtttaaaa gaatagactc atggttgaat ccatttttag atccaaatgg
     5701 atctagctat cagttagcaa atagtttaat tgctattggc tctggcggac ttttaggtac
     5761 tggttttaat gtatcaaaca ttcacgttcc tgttagagaa tccgatatga tattttctgt
     5821 aattggagaa aattttggct ttataggaag ttgtcttgta atattcctat acttttattt
     5881 aatttatcaa atgtataaaa catgttttaa aagcaataac ttatttttca cctatatcgg
     5941 tataggcatt gttgcaatga ttttttttca tgtttttgaa aatataggtg caagtatcgg
     6001 attactacct ctaactggta ttccactccc ctatattagt caaggtggtt ctgccattct
     6061 tagtaatttt ttggggttgg gatttatgtt gtgttgtagt agatatacta acgaaaaaaa
     6121 aggaacacaa aaatgaattc aaaataacac tttatgaata gaatgtctat ttcaataaaa
     6181 attatgatac cttaaagtta atcagtgaac tactcaccac ttagctaaac ctgacggttc
     6241 ttaacgcttg aagtgggagc ttcttgggaa tagagcatac ttgatagcgt atttctttac
     6301 taacagcggt gtctcttatt taccaagcta tccccgtagt tcctacggtt cttgatgatt
     6361 ggatctaagc caatcccata gaatgtagtc ttagaccttc ggtcagaata ttgatactag
     6421 cattgatatc acgatcatga tgagtatgac aaacaggaca agtccattct cgaatttcaa
     6481 gagatttctt gccatcttta tgtccgcact ctgaacaaat ctgactagac ggaaaccatt
     6541 tatctacttt gatgattttt cgtccatacc agtcagcctt gtattgtaat ttagtcacaa
     6601 actttgacca agaaacatca gaaatacttt tagctaattt atgatttcgc aacatacctt
     6661 ttgtgtttaa gtcttcaata cagataatat cgtgattttt gatcatttct gtactcaact
     6721 tattcagaaa atcagtacgt tgattcatta ctttttcatg caatctagct actttgcgtt
     6781 tttgtttttg ataattttta gcttcaaata gattggtacc tttattttta gccaataaag
     6841 cacgcctaga caatttgcgt tgttcacgct ttagtttctt ttccattttg gacgtgaatt
     6901 tattattatc aattttttgt ccatcagaaa gaatcgcaaa gtccataata cctaagtcaa
     6961 taccaattgt ggaattagtt ttaggtaatt cgataatttc ttctttacac aacaaagaaa
     7021 tatagtattt accgctagaa tgacgtgata ttgtagcgga tttgataatc ccttttggtt
     7081 gtctatgaag cttaattctt actaatgact tcaatttagg aactttgatg aatttactat
     7141 caatcaaagc aactgtacca ttttgattat tcgttgtata actctgaaca ggatttttct
     7201 tacttttgaa acgtggaaat ccaacggatt tatcccgaaa aaaattcttg tatgctttat
     7261 ctaaattaag ttgggcattg gctaaagcaa ggctatcaat ttctttcaaa aacggaaatt
     7321 ctttcttgta tttagctggt gtcggaaaag tcattttttt agaagaatca tttttaactt
     7381 cttcatacgc cttttttcgg tcatcaagca ttagattgta gaccttacgg acacaaccaa
     7441 aagatttggc aaagaagatt tcttgttcct ctgttggata gattctgaat ttgtatgctt
     7501 ttagttgttc cataaagttc acctactttc tatttatgat tgtttccttg attttggata
     7561 tacttcttaa tcacatcaat tggtgcgcca ccagttgtca gaagacaaaa acttttagac
     7621 cagaacattt ctttccaaag aaattgtttg actcttggaa agtcacgttt tatcaatctt
     7681 gagctggcac ttttataggc attgatgaat tttgtcaatt ctgttttagg ttgagctttg
     7741 aacataatat gaacgtggtc tttatcatga ttccactcca ctaaagtaat gtgatacgat
     7801 tctgaaattc tttcaaaagt agttttggca taatcagata tttcatcatc aatcacttgt
     7861 ctacgatatt tcgttactaa aacaaggtgg taataaagaa gaaatactga atgattattt
     7921 gtatctaatt ccatgttata tcaactcatt tcttgtatag actgattgta acatggaaca
     7981 aaataaaaag gcaatacgcc ttagttgtcg gtcttcgagt taccaacggt aaccctcata
     8041 ccgaacggca ttcatcaccc acctatagag gatgggcgac ttctgcctaa ttctgttaaa
     8101 ttcaaattat tacaacagtc ctaacgaaga attgtttgta aaacaaacgg ataaataata
     8161 aaaaagactg ctctttcgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag
     8221 caacgcgctt atgaagcgct accaaaaatt tatcatattt tatgaaaaat aacaccccag
     8281 taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaactgca gaaaatcaag
     8341 ccttaaaaag agcttttgac ctagaccaat gaaaaatcac aaagtgagcc cctgtatccc
     8401 ttggtagaga ggctacttgg catagtcgac attatgttaa ctaacgctat aaataacgat
     8461 tatgttccca aaacaatatt ttatgaacta taacaaaaat ttgttatcgc atattgtata
     8521 ttttaattat aggtgtatac cacttaaatt taacacaaag gcgtgattat tttgaaaccc
     8581 aaaaagaaaa tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga
     8641 attacaaata cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa
     8701 gaacaagcaa ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta
     8761 atggaaaata acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc
     8821 gatggagaaa atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat
     8881 acgattaaat atatttatac cgtcaaaaaa aatacaaatg gagaatattc aggaagtatt
     8941 tcacaatttt tagcaaaaga acttcaaaat ctagtagata gtaatataac agatacaacc
     9001 ttattttttc aagataatag catttattat tccgataatg gacaaattaa actactttgg
     9061 aaatcacctt taagtacaga aaaaaatggt gtttcttcct ttacagaaaa aaatgatctt
     9121 atatccgtaa atataaaaaa taaaattaaa aatttctcta aaaaacaaaa ttttcctgcg
     9181 gttaaacgtg taaaaagtgt ctcctccgga gataacttta ttatgattaa ttggcgtata
     9241 gcagaaaccc aaacaagttt tccgtggtgt gctgcttacg tagaagctgc tatcttatgt
     9301 aacaaaacag atgtacatgc tacttcagct agagccatta tgcattatac ctatcctaat
     9361 ttaaacaaca gtcaaattga atcaaaaagt cttaatcaag atcaaataat aaaatatgca
     9421 aattactgtg gtgtttatcc taaaaaagta gcacgaatac taaacctttc agaggtccaa
     9481 acacaactaa gaaaaggaaa tgccatttat atgggaactg ctggttatgg aaattatagt
     9541 aacagccgtc atgcacttgc attatttggg tgggtcaatc aaggtggtta tcaaacttac
     9601 tatgtgtgga atccttggta taactaccca actgtaataa gtgcaaatgc aggaccagtt
     9661 acaattcctg tacctggagg tggttttact tgggacaata ccattacaaa ttggtaaata
     9721 aacccattgc tttagttatg ggtctagtgt tagttctttt cttaactagt tgttcaaaaa
     9781 aacaagaaat acaaaagact gatctgattc cttttctagg aacttggcaa actaaagatc
     9841 attcagaacg tctagtaatc actaatttaa atacttcaaa aaatatagaa caatatacca
     9901 tcttagtaaa agagaacaat cataaaattc aaatcattga taaaaattta aatataaatg
     9961 aacaagtttt catttacaaa gcaacaaata atttactaac actgcgctct aataaaaaac
    10021 aattagatta cacttttagt ttggattctc caaaaaagct cgtatttatt ttgacaacga
    10081 atgatgggac agaagggaca gctaaaccaa ttgaattttt taaagctgat tgatgaatag
    10141 ttgtttgata ttacaaaaca ataatttcac cgaaggtgaa acaagcgaaa ctttacaaaa
    10201 agtggctagc atttgctagc cttttttgta ttgtggagcg tgggttttga gggggattca
    10261 agggggagct tgcgcccctt tgaaaagcgg ttatttgcac caaatacgta ggtatttggc
    10321 tagcaaatat agagcttgcc aagccctttt ggtgagcttt actcattgt
//
LOCUS       DACELF010000087        10019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.091, whole genome shotgun sequence.
ACCESSION   DACELF010000087 DACELF010000000
VERSION     DACELF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.091"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            395..1702
                     /locus_tag="IW687_002233"
     CDS             395..1702
                     /locus_tag="IW687_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0951481.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1821..3020
                     /locus_tag="IW687_002234"
     CDS             1821..3020
                     /locus_tag="IW687_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0951482.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(3047..4315)
                     /locus_tag="IW687_002235"
     CDS             complement(3047..4315)
                     /locus_tag="IW687_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0951483.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(4478..5124)
                     /locus_tag="IW687_002236"
                     /pseudo
     CDS             complement(4478..5124)
                     /locus_tag="IW687_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(5532..6035)
                     /locus_tag="IW687_002237"
     CDS             complement(5532..6035)
                     /locus_tag="IW687_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0951484.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(6158..6922)
                     /locus_tag="IW687_002238"
     CDS             complement(6158..6922)
                     /locus_tag="IW687_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0951485.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            7030..7305
                     /locus_tag="IW687_002239"
     CDS             7030..7305
                     /locus_tag="IW687_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0951486.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            7743..8693
                     /gene="yidC"
                     /locus_tag="IW687_002240"
     CDS             7743..8693
                     /gene="yidC"
                     /locus_tag="IW687_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0951487.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(8872..9072)
                     /locus_tag="IW687_002241"
     CDS             complement(8872..9072)
                     /locus_tag="IW687_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951488.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            9877..>10019
                     /locus_tag="IW687_002242"
     CDS             9877..>10019
                     /locus_tag="IW687_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0951489.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQG"
BASE COUNT         3056 a         2079 c         1791 g         3093 t
ORIGIN      
        1 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
       61 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
      121 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
      181 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
      241 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
      301 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
      361 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
      421 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
      481 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
      541 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
      601 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
      661 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
      721 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
      781 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
      841 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
      901 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
      961 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     1021 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     1081 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     1141 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     1201 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     1261 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     1321 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     1381 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     1441 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     1501 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     1561 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     1621 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     1681 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     1741 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     1801 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     1861 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     1921 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     1981 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     2041 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     2101 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     2161 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     2221 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     2281 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     2341 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     2401 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     2461 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     2521 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     2581 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     2641 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     2701 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     2761 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     2821 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     2881 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     2941 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     3001 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     3061 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     3121 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     3181 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     3241 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     3301 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     3361 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     3421 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     3481 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     3541 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     3601 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     3661 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     3721 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     3781 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     3841 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     3901 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     3961 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     4021 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     4081 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     4141 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     4201 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     4261 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     4321 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     4381 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     4441 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     4501 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     4561 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     4621 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     4681 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     4741 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     4801 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     4861 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     4921 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     4981 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     5041 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     5101 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     5161 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     5221 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     5281 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     5341 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     5401 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     5461 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     5521 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     5581 cttggtgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     5641 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     5701 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     5761 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     5821 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     5881 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     5941 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     6001 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     6061 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     6121 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     6181 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     6241 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     6301 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     6361 ctgtgccata cactgtaaac cctttgtctt tcattaaagg aatcacttct gtcaaatctt
     6421 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     6481 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     6541 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     6601 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     6661 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     6721 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     6781 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     6841 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     6901 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     6961 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     7021 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     7081 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     7141 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     7201 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     7261 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     7321 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     7381 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     7441 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     7501 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     7561 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     7621 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     7681 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     7741 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     7801 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     7861 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     7921 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     7981 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     8041 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     8101 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     8161 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     8221 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     8281 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     8341 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     8401 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
     8461 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
     8521 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
     8581 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
     8641 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
     8701 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
     8761 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
     8821 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
     8881 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
     8941 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
     9001 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
     9061 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
     9121 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
     9181 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
     9241 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
     9301 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
     9361 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
     9421 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
     9481 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
     9541 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
     9601 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
     9661 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
     9721 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
     9781 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
     9841 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
     9901 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
     9961 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaagg
//
LOCUS       DACELF010000088         9986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.092, whole genome shotgun sequence.
ACCESSION   DACELF010000088 DACELF010000000
VERSION     DACELF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.092"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            136..342
                     /locus_tag="IW687_002243"
     CDS             136..342
                     /locus_tag="IW687_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0951490.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(544..1545)
                     /locus_tag="IW687_002244"
     CDS             complement(544..1545)
                     /locus_tag="IW687_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0951491.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1550..3463)
                     /locus_tag="IW687_002245"
     CDS             complement(1550..3463)
                     /locus_tag="IW687_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0951492.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3631..4137)
                     /locus_tag="IW687_002246"
     CDS             complement(3631..4137)
                     /locus_tag="IW687_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0951493.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4297..4737
                     /locus_tag="IW687_002247"
     CDS             4297..4737
                     /locus_tag="IW687_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0951494.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4763..5920)
                     /locus_tag="IW687_002248"
     CDS             complement(4763..5920)
                     /locus_tag="IW687_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951495.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5923..6279)
                     /locus_tag="IW687_002249"
     CDS             complement(5923..6279)
                     /locus_tag="IW687_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951496.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6577..7551)
                     /gene="sgrA"
                     /locus_tag="IW687_002250"
     CDS             complement(6577..7551)
                     /gene="sgrA"
                     /locus_tag="IW687_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0951497.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7747..8586)
                     /locus_tag="IW687_002251"
     CDS             complement(7747..8586)
                     /locus_tag="IW687_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0951498.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8771..9658
                     /locus_tag="IW687_002252"
     CDS             8771..9658
                     /locus_tag="IW687_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0951499.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         3017 a         2007 c         1494 g         3468 t
ORIGIN      
        1 cattaattgc ttatcattag ccatctttgt ttcaagttcg ctagatgatc attttattca
       61 catcctaaca aacttgtcac atttatagat tcttaatcta tttatcatac aattataaaa
      121 aaggaggtgc tgcttatgaa agctatccat atttctttgc tttttttcgc tagtgcattg
      181 attttatttg ctattcatct ttgttcccag ccttatatca ataacaacgg aatattagtg
      241 gaaccagcat tcttttgctt accactcggc tttttgtccc ttcttgcctc cgcaggcttc
      301 ggatttgttg ctttttttaa acgatcaaaa caacaaatct gacttataaa atttacgtaa
      361 aagagatcgt gacaataatc gtgtgatatt cccaaatttc gaataaaagg tgccccttgc
      421 catctctaac tcctcagtca caagttacaa tattcataaa ccatgaaaag cttgtttatt
      481 gaaattgctc cttgtgactt ggagctaagt gacagattac aacctctttt gcatcataag
      541 actttactcc ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt gtttattgct
      601 gaaatcaaac tgatattcat ggttcaaaac agcatttttc tccgcataat ctacttttgt
      661 gactttcacc cccgctttct caaatacaga agttacttct tccatttgtt tcgtaaacgt
      721 atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat gtctgaccaa
      781 ggctaaactt ttgaccttct ctgattgttc aaagtcctta ttatcaaagt aagcccatcc
      841 acatttttca taaagaaagc tagagatttt atcacttact tgagtaagtg cagaaggatc
      901 caacagacca gacaaagaaa taaacttcgt aatctgcttg ttctccaaag tcggatcaag
      961 atgatcagct tttttaattt cttcattttg aagtgctaac aaatattctc cactaatttg
     1021 tgcacctgcc gaatttccac caatcgaaat attcgaccaa tctgcttgat cttttatctc
     1081 ttttttagct gcttgaatgg cttcgttcaa ttggataact ggtgtaggat gttttgcttc
     1141 cggtgatcgt tcataatcta tatttacgat gatttgctgc gtgtctgaac atagatattc
     1201 caaataatca cgacaatcat ttttatcccc tcctacatat gcgcctccat gcatccagaa
     1261 aagaatcggt aattttttct tttccacttt cggtgtgtag atatccatat tattattttt
     1321 gtactttgaa ggataggata tttctttttt tatatcgatt tgttcgattt tttgctggaa
     1381 atcatctggt cgggcatata cttgttcttt ttcacttgtg tcaaattgtt ttctaacaag
     1441 caagctaatc ggctttggtg tgatttgatt aatcaaaaat acagtgacta aaataactac
     1501 gatgataact ggtactgcaa tcactattcg cttgaaccat ttcattcatt taccctctct
     1561 ttcttcgcca aatccatgtg ataatcgtgc taacagataa aaatccacct gaataaatga
     1621 gaagcgaatg tttcttttct ccagttttcg gcaacattct tttttttgca tctgtctttt
     1681 tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc acttgattga tcctctcgag
     1741 gttcattctt tcccttattg tctgcattct ctgtcggatc ttgctttttt ggtggagcta
     1801 ctgggatttt tttgtaatag aaaataatag ttggtatcgt ctcattaaaa atcccttcag
     1861 tcggtccttc aacatttgct aattcgtatc cttcgaatgt cttttgctct gtcatataat
     1921 tttctccata agctccgtaa agctggattt cgtcctggtc tttgagtaga cttttattgt
     1981 tttcatccac atactttacg tttatctgat catggaacac tactttcaaa aagtccgtta
     2041 catgtttcgt gtaggcagca tggtcaactt cctgtgcctt tccatgccct gctccttcta
     2101 ctaataaaag atctttatac cctcctgata actcatataa tttttgcgcg ttagacacag
     2161 gtactacatc atctgctgtt ccatggataa tcaatttagg caatgaatct gacattccaa
     2221 tcgtaggtag agcagctttt gtatccaaat gcatctcctg atcaaaatag tattgtttca
     2281 aaaggcccat aaataaatct tcatgctctt gatccccaat gatgtctaac tgatttttta
     2341 tggcatccgg tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt tgttcatcaa
     2401 ttgatgcaaa tccgcaattt tcaataattc ctttgacatt gacaggaata tctttcgcta
     2461 atacagacat catcgtagct gctcccatcg aaccaccata aagtaaaatc ttttgagatg
     2521 gccgctctcg aacttcttga tttatccagt aaaggacgtc atctgactca tattgtccat
     2581 aggtcacata atcgcctccg ctattaccag tcgctctgga atcaacaaat aataaattat
     2641 acccggcctt ataaaaatca ttgtattctt ctgtaacgat cccgttattc caattccctc
     2701 gaaatccgcc atggatcatg actgttttat ctgaaccttg atcgacataa tatgcatata
     2761 catccatcat tttgccatct tgtttgttta gtacagtttg atgaagttgt tttgtatttg
     2821 gatagaagcc tgtgttttct gtaggcgttg tcccatcatc tgtacctcca agcatcgaca
     2881 tcaacacact gttttttgca aaagcgatat cgtacaatag ataagtagca atcagatgtt
     2941 tgataatctc tttatctaca ccataatcat taacagcaag ctcagtgact aataactcta
     3001 ttaattttga cttgatgtct tctgttggtc cattaagtat ttctgtcatc tgtttaccga
     3061 agtaattcaa atattcttta ccattgaaat tttcaccttt accgatatca tcattttctg
     3121 tcccattctc tttgtcactt actttagcag aagaggattc gtcgtttgac atttttgtag
     3181 ctgttgcttc ttctgtttgt tggttatctg tggtagcggt cgtttcttga tctgtgctag
     3241 taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta ctcgatttcg
     3301 tttcatttcc agcatcagta gtttctgtgg tttctacctc ccgagattcc gattcggaag
     3361 aaatgctctc tccatatact gtccattgcg ggacgcttgt acttacagca aataagatga
     3421 ccaacgctcc ctttaatact tgttttgttc tcaagctatt cactcctttt tatgtttcta
     3481 ggagaattat atgtaaacgc aaacattttt tatacaaaaa ataaatggtt tatcccataa
     3541 aaatagaaca ggattatttt ctcattttac gtaataaagc agaatggata aaccagtatc
     3601 cattctgctt tttttaaacg aagaaacaac ttattgctcc aaaaatgtca gaaatcgttt
     3661 atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag gattatcata
     3721 agttgtcgtt gctcctctac acatcataag ctggtatcct aatccatgag caaacttcac
     3781 agtcgtatct atacaatact ctgcttgtgc cccacataat tcgatcgaat gacagtccaa
     3841 ttcagttaat aaaacactca aattagtctt ataaaatgaa ttcgcatggg tttttctcac
     3901 aaaataactg tgctcaggaa tagataattc cgacaatagc tcccatggtt ttgtttgtgg
     3961 cactaattga gcatcttcgt gttgaacgaa aataactggt ttcccaatcg tttcgtatgt
     4021 tttgattctt ttattcacta atgctgtcac tgtttctaat tcgtgtatcg gcgaagaatc
     4081 aaaacatacc cctttttgta aatcgatcac tactattgcg tcggctgtgt ttgacatact
     4141 tgtgccttct ttcttttttc ttgatttaat agtatttctt atctagctag ttccattata
     4201 cgtgacaaca ttttcttatt ttacacctct aaatggcact tttcgactat ttcttcatgt
     4261 tattatttca taaatactta gctattggag gaatgtatga tgattaaatt gtatacaggt
     4321 tctggctgct cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga tataccgttt
     4381 atcgaacaga acttaattcg tcaaccacta accaaaaatg aatttatcca cttactaaaa
     4441 ttgacaaaca acggcgtgac cgaaatgctc tctactcatt ctattagtta taaaaatctt
     4501 gttcacagaa tagatgaact cagtttgagt gaactatata ttgagattcg tacatcccct
     4561 tctttattga aaaaaccgat tatctacgac caatctgcaa aacttttgac tggatttagc
     4621 gaagataaaa tcaggatgtt cctttctaaa actaaaagaa agcaagagct caacgaactc
     4681 ttgctttctg gtcatcttgt cgattttaaa gaaaataatc tttttgcaat gtcttaattt
     4741 tggcataaac aatctgcttt tcttaattaa cgaaatccaa tttttccaat atggatctat
     4801 ttttctcatt cctgattttt atgatttcag ctttcagacg cgcccaatca ttttttgtag
     4861 gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc atatgccttt ttgtcgatag
     4921 gataatcgct aatgacaata tcaggtaact gatcagcgga tgcactatat tttattgggc
     4981 gttcagtcat agataatatc cttttctttg cccataattc ctgtgtttta ccatactttg
     5041 attgaatata gaatatgaca ggttgttcgt gttttataaa aatatccctg actaacatac
     5101 agtattgaaa aatcaaccgt ctgttctttt cataataagt attaagctct ggaattctgc
     5161 gagctacccc actaaaaaac tgttgcatga ttggatacat cactggaaat atatgctgaa
     5221 aatctctgtc ttctgctgat tttccaaaag catccacctt cttttttgtc ccaaaaaccc
     5281 ttgaatacaa atggatcatt gccaaattga catatagata atttttttct gaagttgtaa
     5341 aacaaatctt gaagtttttt tcaaaatttt ctacaataga cgattcaatc cagccccaac
     5401 tttcttgtac tttatctgga gtatccaaca aatcttcttc caaataggtg ttcattacac
     5461 caaacataaa ataaaggtaa gcttgctctg ttggagtgat gtgattcatc gaccagaagt
     5521 ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt atcaatggat
     5581 gcacaaattt gttcatctct tctgggacat acgtaatcga gtggccttgt ttcattcgtt
     5641 gcaacgaaat tcttagtagg ctgatccatt tctttctatc tatactacgc gtatatggcg
     5701 catgtttcgc aaatttttct aatagttgac gtttattttt tatacttgat aatatatcag
     5761 aatgattttc gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg atacggtatt
     5821 cttctccttc tatcggatta ccttttttca agctgcaaga taaatgatag tttttcaaaa
     5881 tagtattaag ttctcttaat tttctttgaa tcgtacgcaa acttatataa ttttttgact
     5941 aagattttct aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat
     6001 cgttccctct aaaagtatag aataaacttt actcagtaaa aactgagaag aaaactcgac
     6061 ttttatcatc ttgttttctg aaagaaaaga taatttatat tctggatacc tttcgcaaaa
     6121 cgattctgct tgttgaatca ccgaattcat cttagccagg gtgtagttta gatctaaagc
     6181 gatatccttc accacatata atttctcgtc tctttcataa caatagttca aaaattgata
     6241 tacttgcctt tcattgatat caataaattc ttcaaacaaa gtacatactc ctttttatct
     6301 attatttaga cttattccga cagctacatg aataacgtac aaatagattc gttactgatt
     6361 tttcagagat actaccatgc gcagtgtgca ttgtcaatta ttcttgctct tattttccat
     6421 ttgtgttcct gttatttcct gtttctcaaa tatgttagta aacaaaaata gatctggcaa
     6481 agctacaact tcgccaggca atacagcact ctcgaatgaa atattcactc aaaaatctat
     6541 cgttttattc aattttagat ctgttgcttt tattctttat ttcactcgat ggaagagaga
     6601 acgaaaatag cttgctccta caatcaaaat gagtaacaca cccatccatg taattagctt
     6661 gttcaaggtt ctactaccag tttctggtaa atcgcttttt ctattatctg tttctttcgc
     6721 tttttcttga tgatcaattc cacgagaaac aaaatcaggc tgttttagca cactttttag
     6781 gttgctccct gcatttctct tttgttcctt agttggtatg ataggtacca aatcaagttc
     6841 attatttact tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac tctcactact
     6901 catgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgct
     6961 tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt
     7021 gctactttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct
     7081 ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt
     7141 caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt
     7201 actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc
     7261 ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc
     7321 atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt
     7381 gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt
     7441 aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc
     7501 aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac
     7561 tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac
     7621 acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac
     7681 tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt
     7741 catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag
     7801 taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc
     7861 aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg
     7921 ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat
     7981 atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca
     8041 acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa
     8101 aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg
     8161 atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat
     8221 aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt
     8281 caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc
     8341 taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc
     8401 gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc
     8461 aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag
     8521 aattcctaaa acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct
     8581 gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg
     8641 aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct
     8701 acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa
     8761 taaggaaagg atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg
     8821 tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg
     8881 tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac
     8941 actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc
     9001 ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa
     9061 ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat
     9121 gctggctttc caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac
     9181 tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga
     9241 agaagcagat aaagcaaaaa aagaagcaaa tgaaggggaa aatttctcca agcttgttca
     9301 agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac
     9361 caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt
     9421 ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa
     9481 aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa
     9541 aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt
     9601 gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac
     9661 aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct
     9721 tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca
     9781 aaacaaatca ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc
     9841 tatcacatga atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt
     9901 gccgtatcgc ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa
     9961 aaagactaaa aatatccctt ctggac
//
LOCUS       DACELF010000089         9870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.093, whole genome shotgun sequence.
ACCESSION   DACELF010000089 DACELF010000000
VERSION     DACELF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.093"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..267)
                     /locus_tag="IW687_002253"
     CDS             complement(22..267)
                     /locus_tag="IW687_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951500.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IW687_002254"
     CDS             complement(381..959)
                     /locus_tag="IW687_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0951501.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IW687_002255"
     CDS             complement(1036..1566)
                     /locus_tag="IW687_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0951502.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW687_002256"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW687_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0951503.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IW687_002257"
     CDS             complement(2423..2818)
                     /locus_tag="IW687_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0951504.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IW687_002258"
     CDS             complement(2815..4227)
                     /locus_tag="IW687_002258"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0951505.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW687_002259"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW687_002259"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0951506.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IW687_002260"
     CDS             complement(5082..6542)
                     /locus_tag="IW687_002260"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0951507.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IW687_002261"
     CDS             complement(6712..7869)
                     /locus_tag="IW687_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0951508.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW687_002262"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW687_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0951509.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IW687_002263"
     CDS             complement(9336..9863)
                     /locus_tag="IW687_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0951510.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2212 c         1784 g         3366 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt
//
LOCUS       DACELF010000090         9861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.094, whole genome shotgun sequence.
ACCESSION   DACELF010000090 DACELF010000000
VERSION     DACELF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.094"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..534)
                     /locus_tag="IW687_002264"
     CDS             complement(22..534)
                     /locus_tag="IW687_002264"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0951511.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(548..2845)
                     /gene="recJ"
                     /locus_tag="IW687_002265"
     CDS             complement(548..2845)
                     /gene="recJ"
                     /locus_tag="IW687_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0951512.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(2987..3421)
                     /locus_tag="IW687_002266"
     CDS             complement(2987..3421)
                     /locus_tag="IW687_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0951513.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3431..4219)
                     /locus_tag="IW687_002267"
     CDS             complement(3431..4219)
                     /locus_tag="IW687_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0951514.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4216..5157)
                     /locus_tag="IW687_002268"
     CDS             complement(4216..5157)
                     /locus_tag="IW687_002268"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0951515.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5334..5921)
                     /locus_tag="IW687_002269"
     CDS             complement(5334..5921)
                     /locus_tag="IW687_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0951516.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6142..7455)
                     /gene="obgE"
                     /locus_tag="IW687_002270"
     CDS             complement(6142..7455)
                     /gene="obgE"
                     /locus_tag="IW687_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0951517.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7743..8753
                     /gene="gap"
                     /locus_tag="IW687_002271"
     CDS             7743..8753
                     /gene="gap"
                     /locus_tag="IW687_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0951518.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8838..9773)
                     /locus_tag="IW687_002272"
     CDS             complement(8838..9773)
                     /locus_tag="IW687_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0951519.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2709 a         2261 c         1705 g         3186 t
ORIGIN      
        1 agaatagctt cttttttctg cttaatattc cattaatgta atgatgtcat atcctttgat
       61 tttgtcgcgt ccatggagat ccatcaattc gatcaaaaat gcgcagccta cgactacacc
      121 gcctaattgt tcgatcagtt cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc
      181 gtcacaaatc agcacacgtt gacctggttt aatggcatcc ttatgcaatg tcaatgtatc
      241 tgttccatat tctaatccat aagtcacttc gatggtttct cgaggtaact tccctttttt
      301 gcggactggg gcaaaaccga ctcctaactc ataagcaaca ggacatccta cgatgaatcc
      361 acgggcttct ggcccaacta ccatatcaat tcgtttttct tttgcataat ttacgatttg
      421 tttagtagct tcccgataag cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac
      481 aattcctttt gacggatagt cgggaatact ggcaatataa tcttttaaat ccactctttg
      541 tttcctccta ggttgttaac catttcttta tggtaggaat atcgctcaaa agcaaaaatt
      601 cctcaacttt gattttattg agccgtcgct ggtataatct gctctcagtc aaaggatgac
      661 tttctgggct agcgactttt tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa
      721 aaaacacctg tatcataaaa attaataatt tttcagggat acgcaaatat tgagctattg
      781 cttttatttt atgacgtata tcaattcgtt cttgagaatg gatcaattta aacaattttg
      841 cgtactgttc tcttgtgcct accccattca aataggcctc atcaggtgaa atccccatca
      901 gataaacacg attgaatgaa taagaccgaa aaacttcttt cagatccgct aaatcatctg
      961 gacaatccat aacaactaga gagtttggtt ttcttttttc aattattgcc tttacagaat
     1021 ctagtccttc gaaaacaatc gcagcttcct ggataatctc aggcacgaat ttcaacgtcg
     1081 aagaatcaaa tgctaaataa aggctacctt ccgacgttcg gctctgttca cggaaacgtt
     1141 ttgcccgcca atcaaatact tgcagaccac ccacagaaaa atcagatacc atcagctgcg
     1201 gtttcttacg cccgttccat tcattgatcg aaagctttcc taccacatct acctgattat
     1261 tcagcagttc ttcttcttga gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg
     1321 catcggaaag gacaaatttc agatgctgtt ggtttgctcc gattcttttg atattttctg
     1381 cttggacttg tcgaaataaa aactgaggca aaggattatc cgtaccaaat ggcgccaata
     1441 gtttcagctc atcaattcgc tctactgtta cttcatttgg cagcaatact tcatcaattc
     1501 tcaactcagg ccctctcatc aagtcaattt gatgatcgac tatatattgg ttcatcttct
     1561 cctgtagtat agtgacattt tcacttggca tcgttaaacc gactgctgca tgatgtcctc
     1621 cgaagtaagt aaataaatct cgcatctggt caagcatttc aaaaagattc aaggcttcaa
     1681 tactacgtcc agatccttta gctgatccat cttcttttaa agtaaggaca agcgttggtt
     1741 tacccgtttc attcattatt ttcccagcaa caatgcccaa aacgccttca tgccatcctc
     1801 ggtttaccaa tagatggata tgattctcct tattgaccat cgctaatgct tcttgtgtta
     1861 tctgctctac gattgctttt ctttcttcat tgatctcatt caattttttt gcttgaacgg
     1921 atgcttcctc ttcatcaaac gtcgcaagta acgagacggc aggacgtgga tcgcccatcc
     1981 gaccgattgc atttagtctg ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc
     2041 tcatttttac gccgctttct tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa
     2101 tggcatcgat tcctaatgaa acaaggattc gattctcatc agtcaatgaa actaaatcgg
     2161 cgatcgttcc tatcgctact aaatccaaaa attctgcagg cggttcttcc aataatgcag
     2221 aagctacttt gaatgctact ccgactcctg ccaaatctgg aaatggatag tgccccgctg
     2281 gatgacgagg gtgaacaatc gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat
     2341 ctgtaatgat cacgtctaca ccagcttgct gagcgtatgc taccgcatca tttcctgcta
     2401 ccccgttatc aacagtgatg atcaactgga ttccttcttc gatcttttct tggtataccg
     2461 attggtttgg tccataccca tgctcaaatc gatttggcag gaatacttct acttctgcac
     2521 caagtaattc cagtgtttct ttcattaccg ttgcactggt gatcccatct gcgtcataat
     2581 caccgtaaac gaggattttt tctccactga ttacagcttc ttggattcgg ctgattgctt
     2641 tatccatatc gtgtatcaga tacggatcat gtaattcttg ttcctttgga tgtaaaaatc
     2701 ggttgatatc cttttcctca tggtaacctc tttgccacag taacttaccg acaaatggag
     2761 ataatttcag ttcacggatc atttggagaa aggattcaga aggctctgtc tcgcttaact
     2821 tccaatcaaa atttgcttgt ctcactttct cactcctaac ttagttatta tagcaaagga
     2881 aatcagcaaa aaaaagcaga aagaaagaat aaaaacagat cagatccgct agacagatgt
     2941 tcagcaggac ctgatctttt gtacgaccat ttatgaaata tccctattag tcaaaataat
     3001 tgaagctttg tttgggcgat tgagtttttt gttcttccgg cgctttcaat tgactgattt
     3061 gtgcggtttt cgcttgtaac tccgcctgat aagcagcttc caactccgca cgttcgtttc
     3121 taaactcgcg ttgctgttct tcttttgctt cttcaatttt cttttgaagt tctgtttgat
     3181 attctgacat ttcctgagtc agctgtttga tttgcttttt ttgctgccat agtgttgccg
     3241 aagcagtcaa aaatacaatc agtgcaccaa tcagcgcaga accgatgatc actaagatca
     3301 atggcgcaga aaaggaagtg aagccaaaac ttacaggaac atttttattg ttcaatacag
     3361 caaaaagaac aataatcaaa actaaaacaa atccaagaat cacattgctt tgctttttca
     3421 attccttcac ctactttttg ttaaaaattc cgctggctaa ataatcacca atcgttggaa
     3481 acagcgtata aaattttgat gcaacttcta aaataaacgg ttgattgatt tcccgttttg
     3541 gatgtctcat attgcggacg atcatttttg ccaacttggc aggttctaaa acgatttgtc
     3601 cgactttttc caaatagctt ccgcttggat cagccttatc gaagaaattt gtctcgattg
     3661 gacctggatt cactgtcgta acagctacgc caagtggttt caattctaat cgcaaagcgt
     3721 tggagaagcc aaggactgca aatttcgtgg cagaataaac cgttgatttt gcagttgcca
     3781 tttttccagc catagaggcg atattaatga tatgaccttg tcttctttct gccatatcga
     3841 tcgcaaattt ttgtgtcaaa accatcattc ctaagatgtt gacttcaaac atatcgtatg
     3901 cttttccaag gtcgaatgta aggaagtctt cgaaaatccc aaaacccgca ttgtttacca
     3961 gaatatcgac gcgcccgact tcttccatca ccttttcata taattcgtcc acgctttcag
     4021 gattgctgac gtctaccttg aaagcataag cctctttgcc gctcaagcgt ctgcactcgt
     4081 ctctgacacc ttctacaaat gccattcttc gtgcgcaact tataacgatt gctccttgct
     4141 ttgctgcttc ataacagatt tgtgcgccca gaccgcctga actacctgtt actaaaacaa
     4201 ctttatcttc taaattcatg cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa
     4261 atctttcata atcgtcgtgt tttcaaacac ttcttgtgct tctttttcaa gctctaaagc
     4321 tgcttttcct aaatatctcg cactgatatg agtaagtagc agccgttttg cctgcgcttc
     4381 ttttgcaact tcagcagctt gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc
     4441 ttcatcttta ttaaatgtgc tttcatgtac cagaatatcg ctattttccg caagcacaac
     4501 ggaattatgt gtttttctcg tatcgcctaa aatagtcacg attcgtcctt ttttatctgg
     4561 acctacgaaa tcttttccat tgatcgtccg cccatcttct agttggatcg tttcaccttg
     4621 cttcagtttg ccgtacagtg gtccagctgg aatacctaat tcttttagac gatccacctg
     4681 caattctcct ttatgatcat gttcaaccac acgataacca aaactgtcga tcccatgatt
     4741 tagttttttt gtataaacag aaaattgctg atcagtaaaa atcggggctg tttcatttag
     4801 ctcaatgaat ttcagcggat aacttaaacg cgtttgcgaa atacctaaag agacttttat
     4861 gtattcctct atcccttttg gcccgtaaat ctctaatggc gtatccccac cttgaaatga
     4921 ccggctgctg atgagcccgg gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa
     4981 aattttaccg atttttctcg gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc
     5041 gccacagtcg aacaaccaaa cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt
     5101 cacatttcta tgttttgcag gaacacctgc acctgttcct aaaaattgta attccataat
     5161 ctacctgact ttctttaagt tcctctttaa ttttagggta aaaagaaggg ataatcaacc
     5221 gtaagtcgag aatgggttca aattaaaaat aacgttctaa agcgatagcg ttttctttga
     5281 aaattactat aaaaacagca gcgaacccgt ttgagatcct ctgctgtttt ttattaattt
     5341 gaagatccag aactttcatt gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt
     5401 tgtgcagctt ctttcgcatg attttcagct tctttcgcat catgcacatc tttttcgaca
     5461 tcttcataat ccaagcgagt aatttctccg cctaattcca tcattaccaa ttgattcagt
     5521 gggcgattgt cctcttcatc agcaatcaag atcagtcctg tagctccttc accgatttta
     5581 tttcctacaa agtcgaacac ggtctgggca tcttttattt ctttggcatc ttgtgtcgca
     5641 ccgatcattc ctccggcaaa ccatcctaat aaaatcccta gcggaccacc taatacacca
     5701 accaacattc cgattaatcc acccgtagaa gatttgttat taccggtaaa gtcgataaaa
     5761 tcttctattt tgaattgatg tgtcccatta ggatcatggc tgacaactgc catctgttct
     5821 cctttaatcg aacgaacagc atgtaacttt ttgatttctg aaaatgcctg atatgcctgg
     5881 ctttctactt gaaaatttaa aatgattact cgtttgctca ttaaaaacgc ccctttcata
     5941 ccttcttgtt atttatttta cctttaattg aaaaataaga aaagaaaagc actctttttt
     6001 tccaataaaa tagggctgtg acaaaagtcg tatcacagct cctttttccg aataaacggc
     6061 gttcttgaag ctgatcactt ctgtcacaac ctcccttaaa tctgaatcta tattttttgg
     6121 tatttgtttt tatttgcttg cctattcgac aaattcaaat tcaaactcac cgatacgtac
     6181 aaggtcgcca tctttcgcac cgcgtgcccg aagagcttca tccacaccca taccacgtaa
     6241 ttgacgagca aaacgcatca ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt
     6301 ttcgatcttc tcaccagata agatccacgt tgcatctgaa tcacgatcga tttggaactc
     6361 aggaccttct ggattgaatc catagtgtac agtttcttct tccagttctt cttcgtacaa
     6421 tgggaattct ggcgttactt ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc
     6481 ttgtcgagtc actccagaaa ttggaaatac tgggatgtca tcagcaaatt catcttcttt
     6541 ttccttgttc agctgttctt tgaatttcac tagattttcc ggtgcatccg gcatatccat
     6601 tttgtttgcc acgatgattt gcggacgctc tagcaagcgt aagttataag tagataattc
     6661 tttgttgatg gcaagatagt cttcataagg atctcgtcct tccatcccac tcatatcgat
     6721 cacatgtaaa attactcgtg ttctttcgat atgacgaagg aattgtgtac ccagcccaac
     6781 accttgcgaa gctccttcaa tcaagcctgg caagtctgct gcggcaaaac ttctgccatc
     6841 acttgtagta accattccta gattaggtac gagcgtcgtg aaatgataag cgccgatttt
     6901 tggacgagca gatgaaataa cggatagtaa agtcgatttc ccgacagatg gaaaacctac
     6961 aagtccaacg tctgccaata ctttcaattc tagttcgatt tttcgctctt gtcctggttc
     7021 accgttttca gcaatttccg gtgcaggatt ccgaggagag gcaaaacgga tattccctcg
     7081 tccaccgcgt ccaccttttg ccacaactag tgtttgacca ttttcaatta aatctcctaa
     7141 aagagcacca gtctccgcat ctcggacagt ggttccttgt ggtactttaa cataggtatc
     7201 ttctgaacca cgaccgtgca tccctttact catcccattt tcacctggct gcgctttgaa
     7261 atggcgattg aaccgaaaat ccatcaatgt acgtaaccct tcatcgacga tcaagatgac
     7321 atcgccgcca cgaccaccgt ctccgccagc cggtccgccg tcaggaacgt atttttcgcg
     7381 acgaaatgca accattccgt ctccgccctt gccagctttc acatcgatcg ttacttgatc
     7441 taaaaacatg gacataatta agtcctccta ttattcattt atttatttga ctgcctgaat
     7501 ttccacaata aacaaaagtc ttacttattt tatcggtgaa atctgatttt gtctatcatt
     7561 tcagcaaaaa gattgtttac cttcttcttt ttttatcatt tggtaaggtt tccaaccttt
     7621 tttctgctac caagtataat tttttgacaa aatattcttt gcgtagcaga ataacagtgt
     7681 attaattgta ttaattgagg atgccgtttc acagcacccc taataaagga ggaacttttg
     7741 atatgacagt aaaagtaggt attaacggat ttgggcgtat tggccgtctg gctttccgcc
     7801 gcatcaaaga agtctcagat gatattgaag tagtagcaat caacgactta acaagtccaa
     7861 ctatgttagc acaattattg caatttgact ctactcatgg tacttatcct ggcacagtta
     7921 ctgcaacaga agactcaatc gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg
     7981 acgcaagcaa aatcccttgg gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg
     8041 gtttttatac atctgaagaa aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag
     8101 ttatttctgc cccagctggt gctatgaaaa ccatcgtcta taacgtcaat gatgatacat
     8161 tagatgaaaa cgacaaaatc atctcagcag gttcatgtac aacaaactgt ttagcaccta
     8221 tggcttattt cttaaataat gaatttggga tcgaagttgg tacaatgaca acagttcatg
     8281 cctacacttc cacacaaatg ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg
     8341 ctcgttcagc tgctgacaat actatcccac attctactgg tgctgcgaaa gcaatcggct
     8401 tagttatccc tgagctacaa ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg
     8461 atggttcatt aactgaattg gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa
     8521 acgaagcaat caaaaaacac acagttgaca atccttcatt cgggtatgat gaccgtcaaa
     8581 tcgtttctag cgatgtcatt ggaacaactc aaggttcgat ctttgaccca acgcaaacag
     8641 aagtaacaac tgctggtgat ttccaacttg ttaaaactgt tgcatggtat gacaatgagt
     8701 atggatttac ttgccaaatg attcgcttac tagaaaaatt cgctaacttg taatttgata
     8761 gatcaatcga taaaagaagc ctttgactgg atccagtcaa aggcttcttt cttttcatcc
     8821 cctactttta aactcgttca atgaacatat tggcttaaaa attgttggac tacttgctga
     8881 tatttttcag gatctgtctt gtatgcttcc gcatgctcgg ctccttttac aacatacttt
     8941 tcttttggtc ccttcgttgc acgatagacc ttatctaaca tttcaaatgg tacaaatgta
     9001 tcttcgtctc catgtataaa gagcattggg cgctggtttt tcttcaattg ttccactgca
     9061 tttgcttcac cgaaaaagta gccagcacgg acttttgtca ctagactcgt tacttgcata
     9121 agcggaaatg ctggaagact aaacatatcg tctagctgat aagcaagctc atcatgaacg
     9181 gaagaataac cgcaatcttc aatgattgct ttcacattat ctggtagttt ttctccgcta
     9241 gtcatcataa ctgttgctgc tcccatgctg acaccgtata gaacgatttc ttgcgatttt
     9301 ccattgtttt ctagcacttt gtttatccac tgtacatagt ctttacgctc tggccatcca
     9361 aatccaatgt aatccccttc gctttttcca tgtcctcttg catcaggaac aaggacattg
     9421 taacccatat catgaaacat ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag
     9481 ccatgagcca caagagctgt tttttcactt ttcgtttcag caggaagata aatcgctgat
     9541 aatttcagac cgtcttttga agtgatggtc caatcgcttc ggttttcttt tgaactaaac
     9601 catttatgtg catccgtttc tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa
     9661 aagtctttct cagaaggcac aacagcataa ttataaaaat aattccctgc aaacactaac
     9721 gcaacaatca gagctaaagc gacgcctaaa aggattttca tccaaagttt cataaatcat
     9781 ccatctcatt tctctttttt catataacct tgaattagta taatcaaaga atctctacta
     9841 tgcaatgttc atttttataa c
//
LOCUS       DACELF010000091         9859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.095, whole genome shotgun sequence.
ACCESSION   DACELF010000091 DACELF010000000
VERSION     DACELF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.095"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..339)
                     /locus_tag="IW687_002273"
     CDS             complement(<1..339)
                     /locus_tag="IW687_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0951520.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSEL"
     gene            452..1372
                     /locus_tag="IW687_002274"
     CDS             452..1372
                     /locus_tag="IW687_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0951521.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            1445..2380
                     /gene="murB"
                     /locus_tag="IW687_002275"
     CDS             1445..2380
                     /gene="murB"
                     /locus_tag="IW687_002275"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0951522.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            2678..4156
                     /locus_tag="IW687_002276"
     CDS             2678..4156
                     /locus_tag="IW687_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0951523.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4433..6493
                     /locus_tag="IW687_002277"
     CDS             4433..6493
                     /locus_tag="IW687_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951524.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            6576..7145
                     /locus_tag="IW687_002278"
     CDS             6576..7145
                     /locus_tag="IW687_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951525.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7320..8126)
                     /locus_tag="IW687_002279"
     CDS             complement(7320..8126)
                     /locus_tag="IW687_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951526.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8264..9778
                     /locus_tag="IW687_002280"
     CDS             8264..9778
                     /locus_tag="IW687_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0951527.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3533 a         1619 c         1964 g         2743 t
ORIGIN      
        1 taattcgctt tgagaatttg gcgtgtaaca ggttacaaga aagaaatcgg aatattccaa
       61 agtaatcaga cggccttctg tatcatggat atcgataccc attccgtaag tggcattcaa
      121 tgctggtttt ttagcaaaaa tcgctgtgcc ggaatagcct tttcgttccg cgtaattcca
      181 atattgataa taacctggaa gatctaaatc aatttgtcct gcttggagtt tcgtttcctg
      241 cagacagaaa aaatcggcgt ctaactcatg aaaaacttct aaaaaatttt tatttacgat
      301 ggcacgaagc ccgtttacgt tccaagagat aaatttcaat aaaagcacac tccttcgaat
      361 taatttcctt acctttgcga tactattgta cctcatttta gtaaaaaatg gtgtaagatg
      421 aaagtaacta aatgatccag gaggaaagta gatgaagatt ggtgtaatcg gtgtagggaa
      481 tatcgcagaa aaagcatatt taccaactta tgcgaaaaaa caaggaacac ttgattttta
      541 ttttgcaaca agaaataaag aaacaaaaga aagaattcaa aaagagtacg ggttccaaca
      601 tctatatgaa acgatcgatg agcttttaga agaaaaaatc gaagcatgta tgatccacgc
      661 agcaacaaaa gtacattatg aattagccaa aaaatgtcta gaaaatggta tccatgttta
      721 tattgacaaa ccactgagtg tccatctaaa tgaagttcgt gaattgcaag aattagctga
      781 taagaaccaa ataattttga tgatcggttt caaccggcgc tttgctccta tggtagaaga
      841 attgaaacgt atcccagata agcggttgat ccacctgcaa aaaaatcgaa tagcggcaaa
      901 agaaacgaca gagttcgtcc tttatgattt gtttttacat cttattgata cagctgtgta
      961 tttattagat gaacccattt tgagaacaaa ataccagata agagaaacaa aggaattttt
     1021 ggaatatgcc attttacaat tagaaacaca aaatcaaacg gctattttaa cgatggattt
     1081 agcaagtggc gctaatatag aaaaatatca agtaacaagt aaaaatggta cttatgaagt
     1141 aaacgatctg acccagcttg tcattcaaga tgctgatgga aaaaaagtga aagaatttgg
     1201 cgactggaca aacacgctag tcaaacgcgg gtttgagcca atggtagagg ccttcttttc
     1261 tcagatccgt ttaggtaaat cggaaacaga attgcttaaa caaaaagaag ttgtaagaag
     1321 tcatgaatta tgtgaggaag tgcttaaaga tcactaccgt catcaacttt gaacaagact
     1381 ggcaagattg tattaagcgt gatagaataa tgaggacgtt tgagaaaaga aaggaaaatc
     1441 ctcagtgaat aaagaagaaa tagttgaaag cttagccacg attactatat tgtttgatga
     1501 accattgatg aactatacgt ttacgaaaac aggtggacca gctgatgtcc ttgcattccc
     1561 gaaaaaacaa gaagaagtaa agcaaatcat cgattattgc cgaatccatg atataccttg
     1621 gatggtatta ggaaatgcaa gcaatctgat cgttcaagat ggcgggattc gcggtgtagt
     1681 catcatgctg actgaaatga aacagattca tgtcaaagga acgatggtca ttgcggaagc
     1741 aggtgcctca ctgattgata cgacttatgc agctttggca gaatcattga ctggatttga
     1801 atttgcttgc ggcatcccgg gaagtgtcgg tggagcggtt tatatgaatg caggtgcgta
     1861 tggcggagag atcaaagatg tttttgctga agtggatctt ttgcttgaag atggaacatt
     1921 gaaaactttg acaaaagacg aaatggtatt ttcttatcgt cacagtaaag tccaagaatt
     1981 acgtgctatc gtattggaag ctcgattcag tttgcaaaca ggggattatg aagcaatcaa
     2041 agcaagaatg gatgagttga ccgaattgcg tcaatcaaaa cagccattag aatacccttc
     2101 atgcggaagt gtctttaagc gcccagtagg acactataca ggtcagctga tccaacaagc
     2161 aggattgcaa ggcctgaaat ggggcggtgc acaagtctct gaaaaacatg ctggattcat
     2221 cgtcaatatc gatcatgcga cagcgacaga ttatattgaa ttgattgcgc acatccaaga
     2281 agtaatcaaa gaaaaatttg atgtgtcttt agaaacagaa gtgcggatca ttggagaaaa
     2341 acaagcaata tcagaaacaa gcagaagttt agccaattaa taagtaatgg aacttaaaat
     2401 aagtaaaaga gcaggacggt ggccgtcctg ctcttttttg tggttgttta cgcaaatggc
     2461 gcaaatcttg tacattttaa cacaataaaa caaaaaatat agaatatttc ctcataaatt
     2521 tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa
     2581 acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc
     2641 aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga
     2701 agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta
     2761 tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta
     2821 tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc
     2881 agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag
     2941 aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt
     3001 aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag
     3061 aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt
     3121 tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg
     3181 tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt
     3241 tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac
     3301 cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga
     3361 tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt
     3421 tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat
     3481 cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga
     3541 tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat
     3601 tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca
     3661 tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca
     3721 gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca
     3781 agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga
     3841 ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt
     3901 ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg
     3961 atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt
     4021 atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac
     4081 tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac
     4141 tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt
     4201 tatgtttttt tcaaagaaaa ggtaactaga tataactaga tataactaga tatattactt
     4261 aacaaaatta aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa
     4321 aaaaaatact ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct
     4381 tatgaaaatt actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc
     4441 aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg
     4501 ttctgaaaaa cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg
     4561 tgtctatatg catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc
     4621 tgtttctgag accactgatt cccctatcac tattactcat actttcactg atgcaaatgg
     4681 aaaacagcta cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa
     4741 ccactactgg ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc
     4801 gctttctatc ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac
     4861 tgatattttg aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta
     4921 tgtatatgaa aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac
     4981 aaaagtttcg atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga
     5041 tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc
     5101 tactatttcc ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt
     5161 gatccatgtt cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg
     5221 agagagttac gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg
     5281 aaataaagaa gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc
     5341 cgttgtatta acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt
     5401 ttatgtagaa gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac
     5461 aaataaagaa cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg
     5521 cctacctgct gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa
     5581 tgggaaagtc acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca
     5641 ctggtcggag atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg
     5701 aggggaaatt acctttactt atgtttatat accagacaat cgttcattag aaggtcatga
     5761 tcttattgtt catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa
     5821 ttccttaggt aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt
     5881 ggaggatggt tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga
     5941 cccgtattca ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa
     6001 acagatagat caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca
     6061 aatggctcat gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc
     6121 aagaatatct agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc
     6181 cccacaagtt tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga
     6241 aaaagaattt gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac
     6301 gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc
     6361 acttcctagt ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga
     6421 tttcatgcga ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga
     6481 tgactggttc tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt
     6541 tatttggcat aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg
     6601 cagaaatctg gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag
     6661 gttttgaatt tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt
     6721 ccgttgggga atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc
     6781 cttatcctga agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag
     6841 tagcaacctg taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt
     6901 ggttagcagt gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag
     6961 tcttgcaaga atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat
     7021 ggagccatca ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc
     7081 tagacggcac acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata
     7141 aataacgaaa tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct
     7201 catatcttac ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa
     7261 aaagaagggg cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt
     7321 tatcgtttga tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt
     7381 cgactataga ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa
     7441 tccttcatag tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct
     7501 gttttaccat ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag
     7561 acagtgtttc cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt
     7621 tgcatatcga attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca
     7681 actgtcatcg aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt
     7741 tttgtatttt tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg
     7801 ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct
     7861 cctaaaactg ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc
     7921 tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat
     7981 aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg
     8041 atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc
     8101 gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac
     8161 taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga
     8221 aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc
     8281 tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa
     8341 tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga
     8401 acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc
     8461 tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat
     8521 tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc
     8581 ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga
     8641 agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga
     8701 tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac
     8761 gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg
     8821 tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt
     8881 caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca
     8941 tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg
     9001 ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac
     9061 gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca
     9121 aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt
     9181 gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt
     9241 tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa
     9301 atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta
     9361 tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga
     9421 tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa
     9481 aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta
     9541 ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac
     9601 gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga
     9661 agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat
     9721 tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa
     9781 tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc
     9841 tgattgaaca ctgtttagt
//
LOCUS       DACELF010000092         9634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.096, whole genome shotgun sequence.
ACCESSION   DACELF010000092 DACELF010000000
VERSION     DACELF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.096"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            368..1225
                     /locus_tag="IW687_002281"
     CDS             368..1225
                     /locus_tag="IW687_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0951528.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(1296..2153)
                     /locus_tag="IW687_002282"
     CDS             complement(1296..2153)
                     /locus_tag="IW687_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0951529.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(2138..4840)
                     /locus_tag="IW687_002283"
     CDS             complement(2138..4840)
                     /locus_tag="IW687_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0951530.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(4853..6142)
                     /locus_tag="IW687_002284"
     CDS             complement(4853..6142)
                     /locus_tag="IW687_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0951531.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            6292..7338
                     /locus_tag="IW687_002285"
     CDS             6292..7338
                     /locus_tag="IW687_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0951532.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            7340..9493
                     /locus_tag="IW687_002286"
     CDS             7340..9493
                     /locus_tag="IW687_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0951533.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTELYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2767 a         1985 c         1750 g         3132 t
ORIGIN      
        1 cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct
       61 ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag
      121 cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg
      181 attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt
      241 caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa
      301 gttttttctt aaatatgttt taaaaatggt acaattagtc tgcaattgca ctgaaaggag
      361 tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga
      421 cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa
      481 aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca
      541 gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc
      601 gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg
      661 aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc
      721 ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag
      781 cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga
      841 acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat
      901 gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga
      961 taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac
     1021 acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca
     1081 taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat
     1141 gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa
     1201 agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat
     1261 gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct
     1321 aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca
     1381 cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt
     1441 tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac
     1501 gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga
     1561 tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct
     1621 gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga
     1681 tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca
     1741 ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt
     1801 ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa
     1861 gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct
     1921 tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg
     1981 ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga
     2041 tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca
     2101 tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt
     2161 cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct
     2221 tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca
     2281 atttttctaa atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc
     2341 tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg
     2401 aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt
     2461 atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga
     2521 accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt
     2581 ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat
     2641 tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt
     2701 gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca
     2761 ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga
     2821 cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac
     2881 tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt
     2941 ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg
     3001 cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta
     3061 ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag
     3121 gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat
     3181 tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca
     3241 aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt
     3301 ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt
     3361 ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg
     3421 gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata gtggccccac
     3481 tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt
     3541 caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag
     3601 ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg
     3661 tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt
     3721 catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc
     3781 aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat
     3841 ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta
     3901 gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg
     3961 ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt
     4021 cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact
     4081 cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg
     4141 gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat
     4201 ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc
     4261 cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca gggcctttcc
     4321 accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc
     4381 caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt
     4441 gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc
     4501 cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga
     4561 taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg
     4621 cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg
     4681 tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat
     4741 cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat
     4801 cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt
     4861 ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa
     4921 accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg
     4981 ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg
     5041 ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg
     5101 atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg
     5161 ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg
     5221 cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt
     5281 catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct
     5341 cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg
     5401 tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca
     5461 gatatccata acggcaagca tcatcgctag gacggaaacc agaccatgtc attcctgtaa
     5521 aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc
     5581 gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt
     5641 ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa
     5701 gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga
     5761 tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga
     5821 aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga
     5881 tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag
     5941 tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat
     6001 tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc
     6061 agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt
     6121 ttggtatatc tttataaacc actaataaaa ccttctttct atttgatata tctttaaaga
     6181 ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt
     6241 tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta tatgactcaa
     6301 ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct
     6361 gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc
     6421 acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg
     6481 aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca
     6541 cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag
     6601 ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt
     6661 ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct
     6721 cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc
     6781 gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc
     6841 caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat
     6901 ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa
     6961 gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta
     7021 gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat
     7081 gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat
     7141 ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca
     7201 accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg
     7261 ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa
     7321 tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa
     7381 tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa
     7441 ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg
     7501 cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca
     7561 tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag
     7621 ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg
     7681 ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta
     7741 cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat
     7801 agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt
     7861 ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc
     7921 caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg
     7981 atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa
     8041 ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg
     8101 tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg
     8161 ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta
     8221 tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga
     8281 tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat
     8341 gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc
     8401 tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt
     8461 aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac
     8521 ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg
     8581 ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga
     8641 tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca
     8701 gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa
     8761 agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc
     8821 agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg
     8881 aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc
     8941 aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat
     9001 agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag
     9061 ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt
     9121 cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta
     9181 tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg
     9241 tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga
     9301 agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga
     9361 atatacggaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca
     9421 attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc
     9481 aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
     9541 aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct
     9601 tttgaacacc gtttattgag aggttgtgaa atca
//
LOCUS       DACELF010000093         9536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.097, whole genome shotgun sequence.
ACCESSION   DACELF010000093 DACELF010000000
VERSION     DACELF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.097"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..1350)
                     /locus_tag="IW687_002287"
     CDS             complement(58..1350)
                     /locus_tag="IW687_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0951534.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1347..1970)
                     /locus_tag="IW687_002288"
     CDS             complement(1347..1970)
                     /locus_tag="IW687_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951535.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(1973..2809)
                     /locus_tag="IW687_002289"
     CDS             complement(1973..2809)
                     /locus_tag="IW687_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0951536.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2826..3713)
                     /locus_tag="IW687_002290"
     CDS             complement(2826..3713)
                     /locus_tag="IW687_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0951537.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3729..5012)
                     /locus_tag="IW687_002291"
     CDS             complement(3729..5012)
                     /locus_tag="IW687_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0951538.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5695..6378)
                     /locus_tag="IW687_002292"
     CDS             complement(5695..6378)
                     /locus_tag="IW687_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0951539.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6396..7013)
                     /locus_tag="IW687_002293"
     CDS             complement(6396..7013)
                     /locus_tag="IW687_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0951540.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7023..7847)
                     /locus_tag="IW687_002294"
     CDS             complement(7023..7847)
                     /locus_tag="IW687_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0951541.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7840..8400)
                     /locus_tag="IW687_002295"
     CDS             complement(7840..8400)
                     /locus_tag="IW687_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951542.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8574..9530
                     /locus_tag="IW687_002296"
     CDS             8574..9530
                     /locus_tag="IW687_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0951543.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         2838 a         2086 c         1640 g         2972 t
ORIGIN      
        1 atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa tctattctta
       61 ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa tagaatgatt
      121 tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg cccgattcca
      181 cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat ctcctccgcc
      241 atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca tcggatagac
      301 atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg cattgatgta
      361 agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag aaacattgaa
      421 tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag tttcatattt
      481 gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac tgcggtcacg
      541 aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct cattggctga
      601 caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc gactgtaaaa
      661 tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc caccgaatgc
      721 aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat gatgggtaga
      781 tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc tgaaataatc
      841 tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat caccaatcac
      901 atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat aattaaaagt
      961 gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag ctttttcctc
     1021 atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg cacgaatgat
     1081 ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc gtacgtcatc
     1141 cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc catacttgtt
     1201 cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa aaccgactag
     1261 ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg ctctcccacc
     1321 aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc ttttaagcaa
     1381 aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa atcatgctgt
     1441 atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt gacgtttttt
     1501 gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt atcttctttg
     1561 cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag acacaataga
     1621 aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc aactgcaaaa
     1681 ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat ttttcctttc
     1741 ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga aggatacatc
     1801 atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa agtgaacaaa
     1861 catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa aatccgacag
     1921 acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat attcagccct
     1981 tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag aaaatcagaa
     2041 aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc gtactagatc
     2101 cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc ttgtttaaat
     2161 aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg atcgtcgtga
     2221 tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac ttgctgcatc
     2281 cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat tgcatgatct
     2341 ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca tacgtcccaa
     2401 ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt gcaggaatca
     2461 tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg attctcgata
     2521 atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact gcagagatga
     2581 caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc catccttttg
     2641 cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc ataatctctt
     2701 gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg actagaccta
     2761 atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt tcttcctcct
     2821 ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt gacagctccc
     2881 ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt aaaagaaaaa
     2941 gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc accttgggta
     3001 atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac caagttatac
     3061 aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca tttgttcgct
     3121 ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc tgcagcaaag
     3181 atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat catgaccagt
     3241 ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa aatattcact
     3301 aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact gccaccgatc
     3361 aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt cctcgttaaa
     3421 agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa tacatagaga
     3481 aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat ataattatcc
     3541 aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata ataaagtgac
     3601 aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga agcaaatacg
     3661 taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt catgccttcc
     3721 tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc tgcttcttct
     3781 gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc ttgaatttcc
     3841 ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga aatcatttct
     3901 aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg ttctcgaata
     3961 ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc ttcactgttt
     4021 tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt tgccatactc
     4081 agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttct gcgaggtgga
     4141 ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt caactggtta
     4201 cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga cagatccgtc
     4261 gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag atttttacgc
     4321 agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt agatagatct
     4381 tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat acagccagct
     4441 ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat ttgatagctg
     4501 atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt taattgttca
     4561 aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag tccaactaat
     4621 tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat atcaattatc
     4681 ccttgctcca catattcatc taatggttca atctgattga tgtattgagg aaattgattg
     4741 ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa ttttgtgtaa
     4801 tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt ttcttcataa
     4861 agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca agccatgttc
     4921 agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa cataacactt
     4981 aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc cattgttatt
     5041 tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt tcaaggggaa
     5101 taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata ctttcaagaa
     5161 aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt caatgatggg
     5221 aggataatta cgcaaagaaa aaaggatatc agacaactgc ccaatatcct tgacatagct
     5281 aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata actagtaatt
     5341 ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat ggcaacttga
     5401 ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta aattgtcggg
     5461 tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat aaatcaagac
     5521 atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat gtacattaca
     5581 ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt actggagtac
     5641 aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca cctgttattc
     5701 atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa gctctccgct
     5761 acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag caatactttc
     5821 ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac gaaaatggcg
     5881 tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac cagctgctaa
     5941 cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa tatcaactac
     6001 tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga cccctaacga
     6061 accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg tcaaaccacc
     6121 acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag taatcgctgg
     6181 atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac gtatgatcgc
     6241 aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga tcccgcttgc
     6301 ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca tgatttctgg
     6361 cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata ctgattcaat
     6421 tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt cgcataacct
     6481 gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac tttttcatac
     6541 agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc catggactgt
     6601 tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc gttaacaaat
     6661 tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc gaaaagatta
     6721 ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc ttgtcctaaa
     6781 atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg aacaagccgt
     6841 tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt aaaaggagaa
     6901 gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc ggcaaccact
     6961 gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc catgatcaat
     7021 tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg tttggtgata
     7081 tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt gatcttagtt
     7141 atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc attatctgcg
     7201 atccatttag ctccaggctg atctccgtaa cttgcatcca atacagtaga aggataactg
     7261 ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc gctgtatcct
     7321 ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac accttgacga
     7381 ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac agaccggtaa
     7441 gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa tgcttcatat
     7501 tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa ttgagtagac
     7561 tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt ccaatcgtct
     7621 gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc ctttaccttg
     7681 ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct tgttcgttcg
     7741 actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac atagcccatt
     7801 gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg aaaaatcctt
     7861 tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca gattttcatt
     7921 cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat ctgctggcag
     7981 acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta ctttcaagtc
     8041 aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt ttgctgcttt
     8101 gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg caacggcaac
     8161 gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta gcatccattc
     8221 ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat ttttctcgcc
     8281 atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta aaaaattttt
     8341 ttcagtttca aactgttcta ataatacact cataataaga tcaatatata cttgatgcac
     8401 gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac ctcttaaatc
     8461 gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg tgatttttgt
     8521 tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt cagatgaaaa
     8581 aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc gaaagcttca
     8641 atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa gcggaaaact
     8701 tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg ttagacgatg
     8761 ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac catatcttgg
     8821 cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg aataaaaaag
     8881 aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca gaagcgatga
     8941 caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca ggaaaactgg
     9001 gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac cctaaaaacc
     9061 ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg tatgctgtat
     9121 ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca atggttcctt
     9181 ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa aatgaacttg
     9241 ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt gcctttgaga
     9301 acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc ttatttacag
     9361 atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat tacgaaataa
     9421 agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc ttgacaaaag
     9481 atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa ttttaa
//
LOCUS       DACELF010000094         8985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.098, whole genome shotgun sequence.
ACCESSION   DACELF010000094 DACELF010000000
VERSION     DACELF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.098"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..911
                     /locus_tag="IW687_002297"
     CDS             <1..911
                     /locus_tag="IW687_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0951544.1"
                     /translation="KTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQ
                     NVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLK
                     EDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            1152..1745
                     /locus_tag="IW687_002298"
     CDS             1152..1745
                     /locus_tag="IW687_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0951545.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            1758..2657
                     /locus_tag="IW687_002299"
     CDS             1758..2657
                     /locus_tag="IW687_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0951546.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            2660..3133
                     /gene="ssb"
                     /locus_tag="IW687_002300"
     CDS             2660..3133
                     /gene="ssb"
                     /locus_tag="IW687_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0951547.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            3499..3759
                     /locus_tag="IW687_002301"
     CDS             3499..3759
                     /locus_tag="IW687_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951548.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            4204..4410
                     /locus_tag="IW687_002302"
     CDS             4204..4410
                     /locus_tag="IW687_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0951549.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4410..4661
                     /locus_tag="IW687_002303"
     CDS             4410..4661
                     /locus_tag="IW687_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951550.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            4677..5093
                     /locus_tag="IW687_002304"
     CDS             4677..5093
                     /locus_tag="IW687_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951551.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            5075..5347
                     /locus_tag="IW687_002305"
     CDS             5075..5347
                     /locus_tag="IW687_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951552.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            5514..5915
                     /gene="tnpA"
                     /locus_tag="IW687_002306"
     CDS             5514..5915
                     /gene="tnpA"
                     /locus_tag="IW687_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0951553.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            5927..7072
                     /gene="tnpB"
                     /locus_tag="IW687_002307"
     CDS             5927..7072
                     /gene="tnpB"
                     /locus_tag="IW687_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0951554.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            7310..7573
                     /locus_tag="IW687_002308"
     CDS             7310..7573
                     /locus_tag="IW687_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0951555.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            7567..7917
                     /locus_tag="IW687_002309"
     CDS             7567..7917
                     /locus_tag="IW687_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0951556.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(7941..8555)
                     /locus_tag="IW687_002310"
     CDS             complement(7941..8555)
                     /locus_tag="IW687_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951557.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         3313 a         1439 c         1729 g         2504 t
ORIGIN      
        1 taaagacagt cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga
       61 gcgacagaaa agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg
      121 tgacggtaga tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag
      181 gtacgttgat cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat
      241 ccatattgaa agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa
      301 ctttgaaaca aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag
      361 ggattttact ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg
      421 ccaaatggga aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct
      481 tagctaatat ccaaaaattt gtctctcact tgttggaagt cgtaccggga caaatccagt
      541 caacagattt tggatcaacg ttacaagagg tgaaagcagc cagtgaaaaa caggaagcaa
      601 cgcgtcattt aggtgtttgt ccaaaatgtc gggaacaaga agtcttgctt tatcaaaatg
      661 tggcggcatg tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga
      721 aactcacagc cacacaactg aaagagatca ttcaaaacgg ccgaacaagc caatcagtca
      781 gaggactaaa aggtcagaaa ggttcgtttg aagcaacgat cgttttaaaa gaagacttta
      841 caacaggctt tgaattttct gaaaagaaaa aaacgaacta taagaaaaga acacgccgca
      901 ctacaaaata atgaaggggt tatcctatgc gaaatacaac acacaaacaa caagaaatcg
      961 aaaatcaaaa agcaaaaatt cgtttagaac aagagaaatt acgtgcaaat aaaagaagac
     1021 tgcaacaatt aaagaaccag cagcgcattc aaaaagaaat cgaattagat cgatagacgt
     1081 taatcttgtt acaaataaac tactatgata tatttgatgt agtgtatttg taacgaaggg
     1141 aggggaagta aatgcaagat aaactgaaag aattgttggc tattcgcgat ggaaatttat
     1201 ctatgaagga agccaggaaa tttggaatcg cagcaaccac tgtacaaaga ttggtgaatc
     1261 aaggaaaatt gatcaaagtt gatcgtggtt attatgtaga ggatggtcat ggaatagatg
     1321 atttattttg gcttcagcaa cgattttcca ggggaatctt ctctcatgaa acgactttag
     1381 atatttacca gctttctacg aatatgccga aattcattca cttaactttt ccccatggct
     1441 ataacgttga tcgttcaatt acaaaagagc agcagttaca atttcatttc gtgaagaaag
     1501 aagtttatga actaggaaaa gtagagggaa cttcctttca aggaaatcct attactatgt
     1561 atgataaaga gcgtacactt tgtgatattt ggaatccgag atatgaattt gaatatgaaa
     1621 tgaaattaga agcattaaaa gagtacatgg aagacccatt gagagatcca agtaaattaa
     1681 gagattacat gacaaaacta catgtagaaa aagaaatgaa ataccacatg caaagcttat
     1741 attgatggag gaaaaagatg acaccgacac aatttgcagc aaaaacaaga aatttagcaa
     1801 agagtacagg attaaatgca cagttagtgc aacggcattt tatgatggat cgtctaattg
     1861 aacaaatttc tgaatcaaag tacaaaaatg acttcatttt gaaaggcggc tttttattag
     1921 gatcaaaata tggaatcgat agaaggacaa caattgatat cgatacgaca tttagaaata
     1981 gcaaattaac tgaagaaaaa ttgaagagca tttttaatga tttaactaga aaacccacaa
     2041 aagaaggcat tgttttctct attcaaggaa tgaaggaaac aagagaagcc gatttttatc
     2101 ctggatttca aattaaattg gtggctaatt tagagaaagt acgtgtaccg tttaaaatgg
     2161 acgtgacaac aggagattct atttatcctg aagtagatac acactatcac caattaatgt
     2221 ttgaagataa aaaagtggag attccagctt atccaacaga acaaattatt gcagaaaagc
     2281 taagtgctac gttcagcttt ggcacagata attcgagagc aaaagattac tacgacttat
     2341 ttaccattcc gaaactagaa aatatcgata cgaaagagtt atacaaaagt gttcgaaata
     2401 catttacaaa acgtggggat acaaaaccgt tgtcctttta ttatgaaaga gaaatggata
     2461 agatcagagg aaatgactat ttggaaaagc agtgggaaaa atatcgctca gttaacacgt
     2521 ttgcgaattc catttcattt acagatacag tcaacgaaat tgatcaccta atgaaaagtg
     2581 tgatcaaaat tgaaaatcaa gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc
     2641 aagaaatgga gagataaaga tgattaataa tgtgacatta gtaggacgct taaccaaaga
     2701 tcccgattta cgttatactg caagtggcac agcagtagcg acttttaccc tagccgtcaa
     2761 ccgaaatttc gcgaatcaaa acggcaatcg agaagccgat tttattaact gcgtgatttg
     2821 gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa ggcggcttga tcggtgttgt
     2881 cggacggatt caaacacgaa cttatgacaa ccagcaagga caacgtgtgt atgtgactga
     2941 agttgtagca gataactttc aactattaga aagtaaagca gcgacagaga gccgtgcgca
     3001 cgctgatcaa tcaaccacca gtccaagccc cactaccttt gaacaacggg atacaaacaa
     3061 tggattggac gcttcacaaa atccatttgg tggacaatca atcgatatta gcgatgatga
     3121 tctaccattc taagtagcat tttctattgg ttgcatttgc tacggtttat gttataattt
     3181 agtcaacaaa aagaattttc aatctataac caaaaaccac tagctggcgg gctagtggtt
     3241 ttcttttacg tttctattat aagaattaac gggtaggctt actgtctttc ttggcaaatt
     3301 tacaatcaag ccatttgcag atatagtgcg cggctacttc tgccatgatt gcaaaaacaa
     3361 aatcaaaaag aattttcaaa cctataacct cctccccttg ccagattagg ccggggtagc
     3421 agacagcaag actattatag cataattgaa atattcaaat gattggattt ttgctataat
     3481 tttagcgagg tgattgtgat gaacaaatat gttggtttgc ttgataaaat ccgagtaata
     3541 aaaacgcaac cgttattggt tcgctttact ttacaaacga ttcatgaatc gattaattgt
     3601 gtagttgcag atattgaaat tatcgataaa ttgctaatta tggatgatgg aaaatacaat
     3661 attgccgtca caggtcactt caataaacga aaccagctag ttattgctag catgtatgtt
     3721 agaaatcccg atcagtttac gcgattgatg ggcatataaa aaaagaagag ttgcaatcct
     3781 aatgtaggac tgcagctctt tttgttttac atagaaacaa gaaaaatgaa aaggcctttt
     3841 taggaaggcg cgagcgcctt ctcaattgaa aaacctctct cactccaacc gccaccgccg
     3901 ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaagggtct tgcaaagcaa
     3961 gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt atctctttat
     4021 tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt
     4081 aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc tggccaacaa
     4141 atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca
     4201 atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat
     4261 catgcaccac atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat
     4321 cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt
     4381 ttgattgatg agaaaggaaa gaaacgttga tgaaaacaca acacaaatta ttgactgtaa
     4441 aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagctt
     4501 tacttattcc gcggtactgg acgacagaaa cccctttagt agaggatgca ccgactatgc
     4561 tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa
     4621 cttatgaaaa cgaatattgg ttaaccgaat tagagaacta aaaaggagaa aaaaatatgg
     4681 ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg
     4741 aggatatgac attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat
     4801 tgtcggtagc tgtggaagaa atgattgaat caggtgtcga aatgaatctc attgcgacgt
     4861 attatcgaac actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac
     4921 tatgcttgga agtaggcgtc aaaccaagca caatcaaacc ttctgaatac caagctttag
     4981 aactaacgta tgcgaaattt atcgaaacga aaaaagctaa gactgtttca agtgaatggt
     5041 tagattattt tgaaaatatc aataagtatg gcgcatgtta catcatgaag taatcagacg
     5101 gattcaaaaa gactttagtt gtatggaaga aatgaagtac tttgttcaaa atgttgaaga
     5161 acagttatat ttggaaagcg aaacgagtca aacaaaggtc acagtcagcc ataatcgttt
     5221 agatgtgtat aaacgtcgtc ctgaatcatt aaaaaaagga gcggagcttc tacgactaga
     5281 aacagaaaaa tacgtcatga atgtttacct agaaaaaagt gagttggtcg aaaattttat
     5341 ggactagccg agaacacccg tgattttaat catgggatga atcggattca gttgtgacct
     5401 agctttagct aggttaaaca actgacaaat ataaactaaa taatagtgaa atgtcttgca
     5461 ataattgatt ttgtagaaat acatatatta aaataattgc gaggtgtttt tgtatgtcag
     5521 aaattcatta tggacggggt tatgtctatt ctattcaata tcatcttgtt tggtgtgtaa
     5581 aatatcgaca tgatgttttg tatggtcagg tagatattga tgtcaaacag tttttgaaac
     5641 aaattgctga tgataataat ataaaaatta tagaaataga atcagataaa gaccatattc
     5701 atttactcat tgaatgcacc ccacaacact atattcctag tattgttaaa gtatttaaag
     5761 gcgcatccgc tagactgctg tttaaaaaac atcctgaact taaacaactc ctttggggtg
     5821 gacatctttg gaatccaagc tactttgtag ctactgttag cgaaaataca gaagaacaaa
     5881 ttagaaaata tatacaaaat cagaaaaaga aatgaggtga atccgtatgc tacaacatag
     5941 ggcttataaa tttagaatct atcctaatca agaacaagaa atattaatga ctaaaactat
     6001 tggctgttca agatttgtct ataattattt tttgaatttg tggaatcatg aatatacgac
     6061 tactggaaaa ggattatctt ataattcttg ttctgccatg cttccacaaa tgaaaagaaa
     6121 tgcagagaca atttggttaa aagaagttga cagtattgcg attcaatcct cattgaaaaa
     6181 tctttctgat gctttttctc gcttctttaa aaagcaaaat aaacgaccac aatttaaaag
     6241 taaaaaacat cctatacaaa gttatacaac taaaaatgtg aataatagta tcgaaattag
     6301 aaacaatttc gtaaaactac ctaagttagg atttgttaaa tttgcgaaaa gtcaagagcc
     6361 caaagggcga atcttaaatg ctacgattcg aaaaaattcg agtggtaaat tttttgtttc
     6421 catcctgtgt gaagaagaaa tagttgagtt tcctaaatct aattccgcta ttggaatcga
     6481 ccttggtata actgattttg caatcctttc tgatggtcaa aagattgata acaataaatt
     6541 cacgtcaaaa atggcaaaaa aactcaaacg tgaacaacgt aagttgtcaa aacgtgcatt
     6601 actagctaaa aagaaaggcg tcaatctctt tgaagctaaa aattatcaaa aacaaaaacg
     6661 aaaagtagca cgactacatg aaaaagtaat gaatcaacgc aatgactttt taaacaagtt
     6721 gagcacagag attatcaaaa accacgatat tatctgtata gaagacttaa atacaaaagg
     6781 tatgttgcgt aatcataaac tagcaaaaag catttctgat gtgtcttggt ctagttttgt
     6841 gactaagtta caatacaaag ctaactggta cggtcggaaa atcatcaaag tagataaatg
     6901 gtttccatct agtcaaatct gttcaaattg tggacacaaa gacggcaaga aatctctaga
     6961 aattcgagaa tggacttgtc ctgtttgtca tgctcatcat gaccgagata tcaatgctag
     7021 tatcaatatt ttgaccgaag gtctaagatt acatgtcatg ggattagctt agatccaatc
     7081 atcaaaaacc gtaggaacta cggggatagc ttggtaaata agagaaaccg ctgtgagtaa
     7141 agaaatacgc ttgcaagtat gctctattcc caagaatctc gtgactttag tcatgagagg
     7201 ttcaaggatt catggaatgt taatctaatt taatagaaaa ggataattaa gtatatacgt
     7261 tttttcttat gtatgttata ctatgtatat acagaaagag gtgtctacta tggaaatgtt
     7321 acctataaaa aaatggggaa atagtaatgg ccttagattg ccaaagtata ttatggaata
     7381 tcttggaatt catacagaag acaaagtgaa gataatacaa gaagaagtaa acggtcagaa
     7441 gcggctaatt atcgaagcgg ctaatataga aaatgaatta acaattgaac agttatttga
     7501 aaattacact gatgaaagaa atcatgtgac gattcaaaat ttaggtgaag cagtaggtaa
     7561 tgaaaaatgg tgagaaaacc tagacaaggc gatattctat tattgaatac agcacctcga
     7621 tctggacacg aacagacagg taaaagacct tatattgttt taagtcatga tattattgcg
     7681 gattatagta acgttgttac agttgctcca atttctagta caactagaaa ctatccgcta
     7741 tatgtaaata taaactcaga atatgaaatg aagacaacag gaaaagtatt attagatcaa
     7801 ctaacgacaa ttgattatga agccagagag tgtatttttt tggaaaaagc gcatgatagt
     7861 ttagttgagg agttattgat gaaagttaga acagtttttc aaaaagtaag caaataaaat
     7921 agaaacagca gcatgatcaa ctattcgtga ttgaccatgc tgtctttttt tattctatat
     7981 acggtttgtc tcgtaacatt gtagtctttt gcaatcttag cgatcgcaac accttttttt
     8041 agatcctcaa cgatgttttt ataaacaaga cgacgttggg gatcttttgc attggcactg
     8101 tatagcttag gacgtccttt ataaacaccg ttcgcttttg caatcttaat gccttgtgcc
     8161 tgacgtcgtt tagattcggt acgttcttgt tcagcgatca tggccaaaat ttgaatgatc
     8221 aaatctttaa tgaatttatc caggagagga ttgccaattg cttcggccat gatcggtaga
     8281 cttgtgatga tcagctgcac atttttcttc ttcaagtaat tgactgaagc gatgatctcg
     8341 tcgtaatttc ttccaagtcg atcaatagct tccacaataa aaatatcttg atctcggacg
     8401 aaatccagtg cttcttgaaa aacaggtcga tgagtaatcg ttgcgccaga ttgtttctca
     8461 atgaaaattc tctctgcacc gaattttttc atttcttcaa tctgccgttg ttcattttga
     8521 tcgatggatg agacacgtat gtatgcaact ctcaaaattt ttacctactt tctgcaacta
     8581 tttaaaaaac accctaatca tacacgagaa aaccagattt ttcaatgtcg cggtcgtgtc
     8641 tagttagggt ataccttata tagacaaaaa ctaatgatgg aggaaatagt atgagaattt
     8701 tttgtaagtg tctactgata acttattctc aaatgttgta aggattttat tcatttgtta
     8761 atattaaaga acggaactcc aaaaaagtcc gtactctaat ttgcagtctc tcactctttt
     8821 agagtgagag tttttattag attttcaaat taattagtaa agtgatagaa taaattgatc
     8881 atacaaatca aaagaagaag cagggtgata gcatacctat gcttcttttt ttatgtattt
     8941 agctatttct tctttacaat atcgaacata tgtttgtata atgtg
//
LOCUS       DACELF010000095         8748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.099, whole genome shotgun sequence.
ACCESSION   DACELF010000095 DACELF010000000
VERSION     DACELF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.099"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..807)
                     /locus_tag="IW687_002311"
     CDS             complement(310..807)
                     /locus_tag="IW687_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0951558.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IW687_002312"
     CDS             complement(847..1656)
                     /locus_tag="IW687_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0951559.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IW687_002313"
     CDS             1990..2847
                     /locus_tag="IW687_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0951560.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IW687_002314"
     CDS             2840..3742
                     /locus_tag="IW687_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0951561.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IW687_002315"
     CDS             3775..4125
                     /locus_tag="IW687_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0951562.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IW687_002316"
     CDS             4159..5097
                     /locus_tag="IW687_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0951563.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IW687_002317"
     CDS             5175..6068
                     /locus_tag="IW687_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0951564.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IW687_002318"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IW687_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0951565.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IW687_002319"
     CDS             6683..6871
                     /locus_tag="IW687_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0951566.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IW687_002320"
     CDS             6884..7786
                     /locus_tag="IW687_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0951567.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IW687_002321"
     CDS             7818..8060
                     /locus_tag="IW687_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0951568.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IW687_002322"
     CDS             complement(8316..8687)
                     /locus_tag="IW687_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951569.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3047 a         1515 c         1699 g         2487 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaa
//
LOCUS       DACELF010000096         8672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.100, whole genome shotgun sequence.
ACCESSION   DACELF010000096 DACELF010000000
VERSION     DACELF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.100"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(68..1099)
                     /gene="queA"
                     /locus_tag="IW687_002323"
     CDS             complement(68..1099)
                     /gene="queA"
                     /locus_tag="IW687_002323"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0951570.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1292..2467
                     /locus_tag="IW687_002324"
     CDS             1292..2467
                     /locus_tag="IW687_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0951571.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2471..3112
                     /locus_tag="IW687_002325"
     CDS             2471..3112
                     /locus_tag="IW687_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951572.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3109..4026
                     /locus_tag="IW687_002326"
     CDS             3109..4026
                     /locus_tag="IW687_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0951573.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4023..4694
                     /locus_tag="IW687_002327"
     CDS             4023..4694
                     /locus_tag="IW687_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951574.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4890..5724)
                     /locus_tag="IW687_002328"
                     /pseudo
     CDS             complement(4890..5724)
                     /locus_tag="IW687_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5925..6191)
                     /locus_tag="IW687_002329"
     CDS             complement(5925..6191)
                     /locus_tag="IW687_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0951575.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6191..6454)
                     /locus_tag="IW687_002330"
     CDS             complement(6191..6454)
                     /locus_tag="IW687_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0951576.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            6929..7114
                     /locus_tag="IW687_002331"
     CDS             6929..7114
                     /locus_tag="IW687_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0951577.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7256..>8672)
                     /locus_tag="IW687_002332"
     CDS             complement(7256..>8672)
                     /locus_tag="IW687_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0951578.1"
                     /translation="PALYGHQVAISVTIVLLLMMLNLRGLRESASFLLFPVYTFILVI
                     SLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILRAFSSGSSSLTGVEAISNAVP
                     FFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIVPEKEVTVLSQIGKAVFGHGI
                     LYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMPHMYQDRGDRLGYSNGIITLA
                     LGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVVKWKKEGKRWLSKSIANITGA
                     FISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDHYKKVAEQLRLENDAKLHDYD
                     GNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDEDVEKEKEIEAEFKKHFPDVR
                     FSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIPQFVPNRRWQNILHNQTSLRL
                     RLRLSWRENIVVSTYSYHLKK"
BASE COUNT         2540 a         1831 c         1665 g         2636 t
ORIGIN      
        1 ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt
       61 cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa
      121 tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga
      181 ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat
      241 agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt
      301 tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc
      361 catttttacg tacttggttc aatcgctcag ctgcttcttc cgtcaaacgg tagaattcac
      421 tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta
      481 catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt
      541 tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag
      601 tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc
      661 ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc
      721 cgtgttccaa ttcttctttc actaccgctt gcaatctgcc atcaccaaaa gagattcttg
      781 tcccaacttt ggcacgtttc gcaggtttta tcaatgtctc ccacgtatct ccttgtgtat
      841 tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag
      901 caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga
      961 tttcatggaa atgtttatct tccatctctc caattttccg atccaaaaca agtaaacgag
     1021 aatgatcgcg gtcttttaat ggtgtttgtg cgatcaactc ttccggcaag tcaaaatcaa
     1081 aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca
     1141 tgtttttcat tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa
     1201 taaaaaaact accctatttg tattttttca ttgttttagg catactagta atagatagtt
     1261 cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat
     1321 ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt
     1381 catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg
     1441 aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga
     1501 tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca
     1561 catgacgatc cgtgaaaata tcacccttgt ccaaaaattg ttgaaagtca gtcaagaaga
     1621 acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga
     1681 tcgctaccca catgaattat ctggtgggca gcagcagcgt attggagtcg ttcgtgccct
     1741 tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg atcccatcac
     1801 tcgtgattct ttgcaagacc ttgtcaaaga tttacaagag cgcttgggaa aaacgatcgt
     1861 atttgtaacc catgacatgg atgaagcgat taagctagca agccgtatcg ccatcatgag
     1921 tgaaggccgt gtgatccaat ttgatacacc agaaaatatc ttacgtcatc ctgcaaacga
     1981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     2041 agtcggagaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     2101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     2161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aagcgaggaa aagtatccag
     2221 tgtcggcgac atattaaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     2281 taccttgcaa cgcattttaa aacgcggact gaaatacgtg ccggtcgttg atgaacagaa
     2341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     2401 gggagaagat cccgcaacta ctgatgttgc agagtcagat actcctgaag aatctaagga
     2461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     2521 tgaacatctt tatatttcgg gacttgcttt gatcattggt atcatcattg ctgtaccgct
     2581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     2641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     2701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     2761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     2821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     2881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     2941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     3001 tttggcaggt acgatccctg taacgatttt agcactattg gctgattggc ttctaggtct
     3061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     3121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc tttccctggt
     3181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     3241 cagatcttag caagcctagt tgccggaatg gttgaacatt atacagataa aaataccgca
     3301 atcatcaaca atctggctac cacaactatc aaccatcaag cgatgatgaa cggagatgct
     3361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     3421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacgat
     3481 caaacgtggt tcccgtccta tggttttgaa aatacgtatg tctttcttgt tcgaaaagac
     3541 accgcacaaa aataccattt atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     3601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     3661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     3721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     3781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     3841 ccagttgtaa acaacaaaat attagctgat acgcctcatt taaaagaagc cttaaataag
     3901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     3961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
     4021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
     4081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
     4141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
     4201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
     4261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
     4321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
     4381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
     4441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
     4501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
     4561 gaaccaatgc aacagatggc acatcgatta ttttagcagg tgcattgcca actgcattaa
     4621 tggtgattgt cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
     4681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
     4741 aaggcttttt tagttctgtc attttttaag gcaataatcc actctgattc ttccctcctt
     4801 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
     4861 gccaacgggt tcatctctgc atccaccgac taagtttttt tcttatccgc taaggcattt
     4921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
     4981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
     5041 agccttttcc atcagtcctc gattgttcga tgccttcagc tacatcatca aaactggtga
     5101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
     5161 tggctaaaaa tatatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
     5221 accggtacat gattcttatg gactgcatcc gtcgcaacta gacttggcgg tacgatgatc
     5281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
     5341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
     5401 cgaaagcgcc acgactttga cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
     5461 tttggatacc cgctctgcta aagaaacgac actggtattg tatttcgtat ctgtatcttt
     5521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
     5581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
     5641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
     5701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
     5761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
     5821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
     5881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
     5941 caggcagaat aaatttccac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
     6001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ctcaaaagac
     6061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
     6121 aaattcgatt gcttgatttt tcttaaatcc actgtcgcag agtttttgat tgcgactgta
     6181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ccactgtcgt
     6241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
     6301 gaatcgctct ccagtcttct agcccaatca atacagcatt gtttccacta cgatcactaa
     6361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
     6421 gagctgtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
     6481 ttataacgta cttgattaag tacgtcaaaa aatacaataa agcacttaaa ttcagcaaac
     6541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
     6601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
     6661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
     6721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacccacgaa
     6781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
     6841 tgattgcctc tttcgtctcc tgccattcat gttcactatc gatttgatac ttgcttagac
     6901 cgtcgggttt gtgaatcacc aattaaaaat ggcactgaat tatggtaaca aaaaatggtc
     6961 acttgacgag gctgagattt ctacttatga agacacatgg cacttaagag tcgaggtaaa
     7021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
     7081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
     7141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgggaataa gacaaaacag
     7201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
     7261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
     7321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
     7381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
     7441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
     7501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
     7561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
     7621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
     7681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
     7741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
     7801 accgatagac gaacaaaatc gcaatgattg catatgagat gaacgcacct gtaatattgg
     7861 caatcgactt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
     7921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
     7981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
     8041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
     8101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
     8161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
     8221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
     8281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
     8341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
     8401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
     8461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
     8521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
     8581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
     8641 aaatagcgac ttgatgaccg tagagtgctg gc
//
LOCUS       DACELF010000097         8546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.101, whole genome shotgun sequence.
ACCESSION   DACELF010000097 DACELF010000000
VERSION     DACELF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.101"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            453..1025
                     /locus_tag="IW687_002333"
     CDS             453..1025
                     /locus_tag="IW687_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0951579.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1257..1874
                     /locus_tag="IW687_002334"
     CDS             1257..1874
                     /locus_tag="IW687_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0951580.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="IW687_002335"
     CDS             2102..2440
                     /locus_tag="IW687_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951581.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /locus_tag="IW687_002336"
     CDS             2425..3279
                     /locus_tag="IW687_002336"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0951582.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="IW687_002337"
     CDS             3426..3659
                     /locus_tag="IW687_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ0951583.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3857..5761
                     /locus_tag="IW687_002338"
     CDS             3857..5761
                     /locus_tag="IW687_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0951584.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKEFTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            6117..6482
                     /locus_tag="IW687_002339"
     CDS             6117..6482
                     /locus_tag="IW687_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951585.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            6499..7110
                     /locus_tag="IW687_002340"
     CDS             6499..7110
                     /locus_tag="IW687_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ0951586.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILDL"
     gene            7138..8463
                     /locus_tag="IW687_002341"
     CDS             7138..8463
                     /locus_tag="IW687_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase P"
                     /protein_id="HAQ0951587.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNAVLLQSFKSTIK"
BASE COUNT         2788 a         1368 c         1868 g         2522 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaaa
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtgaaa tcagttaacg gtattgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa accaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg ttcttaaagc aaaagtcaag gccgtttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacgttct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaatttac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 agtggatgat atagatgaag gggatatctt gctcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggtgaagc
     4381 tattccggtc aacaagctag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggaa aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcggaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagtaatgt ggatacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg ggaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa ggaactgaag ttgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc tcagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggctacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcagttg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcgg aaagaacaaa aagaagtatc agcacctaca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa ataagagtat
     5881 agagtatgtt ctttataaaa ttaattttcg tgtaaatgat taaaaaattt aataataaaa
     5941 tttaaattcc tattaagttg taacacatga aattgcttaa aatagtgtat caaaaacctt
     6001 gttttattaa tgttgaattt atttgaaagg tctacagatt aacatattat tgacaaaaca
     6061 atatttaaat agttatactt cattcaaacg tctgtttgaa cgaggtggtg aagttaatga
     6121 ctgcagaagt atgtacaact acttgtatac ataaagaaaa agtagctagt gttaaaaata
     6181 atttaaaaga taaagacctt tcaactttac ttgtattagg aaaatgtttt agcgattctt
     6241 ctaggattaa aattttctat gctttagaaa cgtataagga gatgtgcgtt tgtgatttag
     6301 cagcgatact taatgctagt attgctacaa cgtcacacca tttacgtttt ttgaaaaaga
     6361 acggaatggc gaaatcacgt caatatggaa aggtagttta ttactcttta gcgaatgaag
     6421 agattctttc cgctgtgcag gcttttctaa agttgtcaga aaatagttct atgaaagctt
     6481 agtttggagg tagttatttt gttacaaaac attttatcag cactcgctgt atatatttct
     6541 accagtattg attatttatt tatcttgctg attatttttt ctcaaaacca tactaaaaaa
     6601 ggtctccgtc agattttttt tggtcaatat cttggaacag gaatcttggt agccataagt
     6661 ttgtttgcag catatgtgct aaattttatt cctcaagatt ggatcattgg tcttctggga
     6721 ctaattccga tttatttagg gattcgtgtt gcttttgtgg gcgaagagga ggaagaagaa
     6781 ggggaagtcg ttgagaagct aggatctaga ggaacgaatc gtttgttttg gacagtttcc
     6841 ttaataacga ttgcttctgg tggcgataat ttagggattt atattccttt ctttacctcg
     6901 ttatcttttt cagaaattgt cacttcttta atcgtctttg ctatctctgt tgcggttctt
     6961 tgctatatta gttataaatt tgcgaaaatt tcttttgtct ctgaaacact agaaaaatat
     7021 gaacgaatca ttgtacctat cgtatttatt ggattaggga tttttattat gatagaaaat
     7081 ggaaccatcc aaactatatt agatttgtag agatcttatt agtaaaggaa gcaaacgatg
     7141 agtgaaaata aaaagtacca tgaatggata ttagaaggaa tcgactgtgc aaattgtgca
     7201 gacaaagtag aaagaggcgt cgccaaagtt gcaggtgttg ccaatagtag tgtgaactat
     7261 atgacagaaa ccttgagttt cgaagtgaca gaggaaaaag aatatcttgt tttatccaat
     7321 gtgcaacaaa aagtaaaaaa attggagcct gatatcactt taaaaaataa agtggacggt
     7381 aggctgattg aaataaatcc ggcattaatc aagaaaagta atttaaaaga cgataaaaaa
     7441 aactacgaat ggatattaga aggaatcgat tgtgcaaact gtgctgccaa agtggaaaga
     7501 ggagttgcta cagttcccgg cgttacaaac agcagcgtca attttatgac agagacactg
     7561 agcttcgagg tagcaggtga aaataaaact gacgtgttgg ctagtgtaaa acaaacagta
     7621 ggaactttag aacctgacgt tgttctgcga aaaaaatcgg acggtacatt gattgaacat
     7681 ggaactgact ctaataaaaa ttcgacttct aacaaatcga tttctgataa agatgagaca
     7741 aaccaaaagc agacgaatag agcttatctg gccagtcttc tcggaaataa aactaaaatt
     7801 acccttattc gtttaatact aggattagga atattagcga caggaatatt tctccctatg
     7861 gatggacaag cctctcttgg gttgttcatt ctggcttatt taatcaccgg ttatgacgtt
     7921 gtaggacgtg ccattagaaa tattttccga ggtcaagtat tcgacgagaa ctttctaatg
     7981 accattgcga cgttaagtgc cttctatatt caagagtatc ccgaagctgt agcagtcatg
     8041 ctattttatc aagtgggaga agtattccaa gatattgcta tttcaaaatc tcggcgctcc
     8101 attgcggacc taatggatat tcgacctgat tatgccaatt tagtcacagg aaacaacgta
     8161 gaaaaagttg ctcctgaagc aattaagatt ggaaatatta ttctcattcg tccgggcgaa
     8221 aaagtgccat tggatggaaa agtagtggaa ggtgcttctg cgatggacac ttctgcttta
     8281 acaggcgaat ccgttccaag aagcgtgaag ccggaagaca ctattttgag cggatttatt
     8341 aataaaaacg gattgctaaa agtagttgta gaaaagccct tttcggagtc tactgtgaag
     8401 aaaattttag atttggtcca aaatgcggtt ctgttgcaaa gttttaaatc tactatcaaa
     8461 taaggtagaa taatagaaaa agatagtagg aggaatgacg atgaatcatt ttaaaggaaa
     8521 gcaatttcag caggatgtga ttattg
//
LOCUS       DACELF010000098         8480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.102, whole genome shotgun sequence.
ACCESSION   DACELF010000098 DACELF010000000
VERSION     DACELF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.102"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="IW687_002342"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="IW687_002342"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0951588.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="IW687_002343"
     CDS             complement(1023..2126)
                     /locus_tag="IW687_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0951589.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3151)
                     /locus_tag="IW687_002344"
     CDS             complement(2216..3151)
                     /locus_tag="IW687_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0951590.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="IW687_002345"
     CDS             3453..5387
                     /locus_tag="IW687_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0951591.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5844..6260
                     /locus_tag="IW687_002346"
     CDS             5844..6260
                     /locus_tag="IW687_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0951592.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6274..6840
                     /locus_tag="IW687_002347"
     CDS             6274..6840
                     /locus_tag="IW687_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0951593.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6863..7849
                     /locus_tag="IW687_002348"
     CDS             6863..7849
                     /locus_tag="IW687_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0951594.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8004..>8480)
                     /gene="scm"
                     /locus_tag="IW687_002349"
     CDS             complement(8004..>8480)
                     /gene="scm"
                     /locus_tag="IW687_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0951595.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2488 a         1637 c         1723 g         2632 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc ttcttcgaaa
     5581 aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct tcttatttct
     5641 tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc tgctgcttct
     5701 cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct tcgttcttat
     5761 tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat tgggtgagtt
     5821 aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa agtctgctca
     5881 aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta ccagtcatga
     5941 tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg cgtgtggatg
     6001 gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca tatacttaca
     6061 aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta cggataaaag
     6121 aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa aatggcgaaa
     6181 ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat ggaggtgacg
     6241 aagacagtct gatttattag accttcacac accatgagta aattacaaaa aatgatttat
     6301 atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat gatcccttat
     6361 cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac gatcgttgcc
     6421 atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact attcctgact
     6481 actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc tttgcttagt
     6541 tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat catcggaatt
     6601 agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc tttcattgca
     6661 caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat tttatcaggg
     6721 attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact tcaacgattc
     6781 caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac caaaaaataa
     6841 tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact atcctgcttt
     6901 aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc tgaaaaacaa
     6961 acctgtcgaa caagcaatca aagaaacgat ccatgccggc ggaaagctgt tgcgtcctgc
     7021 ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag ccatcgcttt
     7081 ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata tcgttgatga
     7141 agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg tcgtttctgt
     7201 ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt actccacttc
     7261 cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg gcgaattagg
     7321 gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg aaaatatttc
     7381 aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg aaagtggaac
     7441 aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga tggcctttca
     7501 gattatcgat gatattttag attatacaaa agagagccag gaaatcggca aacctgtact
     7561 agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc aaaaaaacaa
     7621 accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag atgtcgacgc
     7681 cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt tagcggcctc
     7741 ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc ttcgaacaaa
     7801 agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa tagggaaagg
     7861 ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca aattgaattg
     7921 tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta gcgacgttgc
     7981 tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat aaaagtgatt
     8041 cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt cgattgactt
     8101 ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt tttattagtt
     8161 aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact tgcatgacgt
     8221 gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga atctgagcta
     8281 tcacttgtgg tagaggatgt atctgtactg tcacttgaag ctgacgtgtc cgtgctatca
     8341 cttgtagtgg tagatgaatc tgagctatca cttgtggtag aggtagtatc tgtactgtca
     8401 cttgaagctg acgtgtccgt gctatcactt gttgtggtag atgtatccgt actgtcgctt
     8461 gaagctgacg tgtccgtgct
//
LOCUS       DACELF010000099         8425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.103, whole genome shotgun sequence.
ACCESSION   DACELF010000099 DACELF010000000
VERSION     DACELF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.103"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            471..2108
                     /locus_tag="IW687_002350"
     CDS             471..2108
                     /locus_tag="IW687_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0951596.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW687_002351"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW687_002351"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0951597.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IW687_002352"
     CDS             complement(3184..3819)
                     /locus_tag="IW687_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0951598.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW687_002353"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW687_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0951599.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IW687_002354"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IW687_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8425
                     /locus_tag="IW687_002355"
     CDS             6417..>8425
                     /locus_tag="IW687_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0951600.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2800 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaat
//
LOCUS       DACELF010000100         8391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.104, whole genome shotgun sequence.
ACCESSION   DACELF010000100 DACELF010000000
VERSION     DACELF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.104"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..973)
                     /locus_tag="IW687_002356"
     CDS             complement(131..973)
                     /locus_tag="IW687_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0951601.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1010..2476)
                     /locus_tag="IW687_002357"
     CDS             complement(1010..2476)
                     /locus_tag="IW687_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0951602.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2532..4376)
                     /locus_tag="IW687_002358"
     CDS             complement(2532..4376)
                     /locus_tag="IW687_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951603.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4978..5928
                     /locus_tag="IW687_002359"
     CDS             4978..5928
                     /locus_tag="IW687_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0951604.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5973..6606)
                     /locus_tag="IW687_002360"
                     /pseudo
     CDS             complement(5973..6606)
                     /locus_tag="IW687_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6866..7414)
                     /locus_tag="IW687_002361"
     CDS             complement(6866..7414)
                     /locus_tag="IW687_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951605.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7415..8272)
                     /locus_tag="IW687_002362"
     CDS             complement(7415..8272)
                     /locus_tag="IW687_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0951606.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT         2508 a         1711 c         1314 g         2858 t
ORIGIN      
        1 agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata acaaacagct
       61 ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca tcaatagtat
      121 tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca cctaggtata
      181 caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct ttgatcttag
      241 ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat tcttcctcac
      301 catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca tgtgtaatga
      361 agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac cggaccaaat
      421 tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca ggatcttccg
      481 attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga agatcaattc
      541 catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct ttgggatagt
      601 agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt gcgcgttcaa
      661 ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt tttttatcta
      721 atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca ataggtattt
      781 cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga aatattttat
      841 ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc cctttaccta
      901 atgcaatcat ttctttgcca ttttcttcaa ccagtaccac acttgaattc aatacttttt
      961 ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac tactcgaata
     1021 aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc tttttcaagc
     1081 ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca tatcgtttag
     1141 acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta taagctatca
     1201 aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta aagtagtcga
     1261 tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc ataccatttt
     1321 ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa attttcaaac
     1381 cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc ttaaccccta
     1441 aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc atggacatgt
     1501 aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga tcttcatctt
     1561 ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca ggatattctc
     1621 ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag gtcagctcaa
     1681 cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata caaccaactt
     1741 ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca acaaattgat
     1801 gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc tcttggataa
     1861 ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat gtcagccaat
     1921 attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca aaatcatcaa
     1981 ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc atttcataat
     2041 gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag acgttttcat
     2101 aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac aagcgagtcc
     2161 aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct aaatcttctt
     2221 tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca gatttgtctt
     2281 cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa tctttgttgt
     2341 ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg tctacattcc
     2401 aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct tttggaaaac
     2461 ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata ttttttttca
     2521 ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt ggttaccaat
     2581 ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac acagataact
     2641 gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag cttttgtcct
     2701 ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc taactgaaca
     2761 gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa accaatagca
     2821 tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa tatcccttct
     2881 tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt gtcttttact
     2941 tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc tgaaacaacg
     3001 gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata aagaaccaac
     3061 gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca aaccagattc
     3121 attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc cactaaagca
     3181 aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga aaacataacg
     3241 gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc agtaattccg
     3301 aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc ttttgttttg
     3361 atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga agccgggaga
     3421 taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat cattgcttta
     3481 tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt tgcaacccat
     3541 ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac attataacct
     3601 aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat cataggtaca
     3661 aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag ttgagcataa
     3721 aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt atccaatcca
     3781 aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag gatcaatgca
     3841 ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt agtcattgcc
     3901 accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat ctgataggtt
     3961 tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga cttcaatact
     4021 cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa atctaataga
     4081 actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac attgtcacct
     4141 aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat tacttggcat
     4201 tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct catatttacc
     4261 ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa atgggatatg
     4321 ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta attcatggtg
     4381 tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat cctcaacaca
     4441 aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga aacgcataca
     4501 ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg tttaagttat
     4561 attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt aatcattttt
     4621 tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt ttctaagaat
     4681 atcttccaac atatataact cttattctct tctatcttct agcagatgtt ctgtcttttt
     4741 aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga ggttttttat
     4801 ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta ctaaaaaatg
     4861 ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata tcgatattgc
     4921 ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt ttataagatg
     4981 aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat cagtaattta
     5041 gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg aagaacaaat
     5101 gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat caatactcgt
     5161 ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc ggtaaaagat
     5221 gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac agattccatt
     5281 ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca aatgaaaact
     5341 gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac tggagttgga
     5401 aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt tattcatgag
     5461 aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga attattgtcg
     5521 gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga tcgaaatgga
     5581 ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag attgcctcct
     5641 gaagggcaag agaaactatt tgtttatatg gatggcggaa cttattctag agtcggtgaa
     5701 actatccatc acgcctccaa tgtacgttta gtttttgcga ctacagaagg caaagaggat
     5761 tttctggaaa cctttatgcg aagaatccct atacatatcc atatcccaaa tattgaggaa
     5821 cgaggtattt tagaaaaaaa acaaattatc gaaaatactc tctttagcga aagcaaaaac
     5881 tttagcaagg ctattgatgt ttctcctaaa gtcactagtg ttgcataaat atagtcagaa
     5941 tattctgaat tattattttc ttgaaaaaag taaaaaaaat cgtatcccaa actttcatct
     6001 acatttccag aaagatcttc atgttgataa tcaattcctg aatcaatgac cgctaccttc
     6061 acatccttag aacccttggt ttcttcccaa gcggatggca tgctaatctc tcttaaatta
     6121 gcttgtttac tccaattagg atcgttatcg atttcctaaa gaatcagttg tatttggctc
     6181 ctcaacatca ttcaacggct ctagttcaac cacatagttt ggctctgcat aattgaccat
     6241 atcagaagca ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac
     6301 taataatact tgatctcgct tattataatc gctttcattt gtcctaagag tcaaatcttt
     6361 ttccaccata acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct
     6421 ttgtgtagcc tttaaactga caattagctc attgggtaca tactctattt gagagtcatc
     6481 taattggctc ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc
     6541 catgaaccca aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt
     6601 tttcatctac atctcctcct aatagtttta ttttagagtt agctctatta cttatttact
     6661 aactcaacaa cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca
     6721 ccctaaaatc ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa
     6781 aaataaatcc cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg
     6841 tagttactta gttcttcttt gattattatc taactttatt attcattccc caccctttgc
     6901 gcagtgagtc ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat
     6961 catctaattc tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt
     7021 cattaaacaa atcttgttta tcttcttgcg taagattctg tttaatatac tcatctaaag
     7081 cttttctaag caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg
     7141 aagtcgcatt taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa
     7201 actttctgat tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg
     7261 aggaagtttt tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa
     7321 ttgcttgctg ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt
     7381 tcccaaaaat atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct
     7441 cgtgttcttc caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt
     7501 gttttaagag acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta
     7561 agcgcgcatt tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt
     7621 gtttatacat ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag
     7681 caacatataa atttgtatca aattctttca caaaaaagtc catatactca agatattgca
     7741 tcgcatttct catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt
     7801 acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa
     7861 actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat
     7921 tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat
     7981 agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt
     8041 cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga
     8101 agttcatatg cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta
     8161 tcaaggttct ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac
     8221 ttttacctac gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac
     8281 ctcttttctt ttagcaatat tcatttgata ctattataac atatttattt ttagttacta
     8341 ccaaaaaaaa gatatatatg tactacctta taattaataa gtaaaaggta g
//
LOCUS       DACELF010000101         7400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.106, whole genome shotgun sequence.
ACCESSION   DACELF010000101 DACELF010000000
VERSION     DACELF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.106"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..151
                     /locus_tag="IW687_002363"
     CDS             68..151
                     /locus_tag="IW687_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0951607.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IW687_002364"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IW687_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0951608.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IW687_002365"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IW687_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IW687_002366"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IW687_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IW687_002367"
     CDS             2307..2552
                     /locus_tag="IW687_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0951609.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="IW687_002368"
     CDS             2655..3524
                     /locus_tag="IW687_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0951610.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="IW687_002369"
     CDS             3505..4239
                     /locus_tag="IW687_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0951611.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW687_002370"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="IW687_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="IW687_002371"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="IW687_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0951612.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="IW687_002372"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="IW687_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0951613.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6718..6906)
                     /locus_tag="IW687_002373"
                     /pseudo
     CDS             complement(6718..6906)
                     /locus_tag="IW687_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6886..>7400
                     /locus_tag="IW687_002374"
     CDS             6886..>7400
                     /locus_tag="IW687_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0951614.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2133 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga
//
LOCUS       DACELF010000102         7187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.107, whole genome shotgun sequence.
ACCESSION   DACELF010000102 DACELF010000000
VERSION     DACELF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.107"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW687_002375"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW687_002375"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0951615.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW687_002376"
     CDS             complement(1172..1867)
                     /locus_tag="IW687_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0951616.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW687_002377"
     CDS             complement(1880..2596)
                     /locus_tag="IW687_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0951617.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IW687_002378"
     CDS             2926..3534
                     /locus_tag="IW687_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0951618.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW687_002379"
     CDS             3859..4182
                     /locus_tag="IW687_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0951619.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW687_002380"
     CDS             4364..5734
                     /locus_tag="IW687_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0951620.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IW687_002381"
     CDS             6081..>7187
                     /locus_tag="IW687_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0951621.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACELF010000103         7179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.108, whole genome shotgun sequence.
ACCESSION   DACELF010000103 DACELF010000000
VERSION     DACELF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.108"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..219)
                     /locus_tag="IW687_002382"
     CDS             complement(<1..219)
                     /locus_tag="IW687_002382"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0951622.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="IW687_002383"
     CDS             complement(330..584)
                     /locus_tag="IW687_002383"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0951623.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="IW687_002384"
     CDS             complement(777..1052)
                     /locus_tag="IW687_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0951624.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="IW687_002385"
     CDS             complement(1024..1977)
                     /locus_tag="IW687_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0951625.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="IW687_002386"
     CDS             2589..4082
                     /locus_tag="IW687_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0951626.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..4513
                     /locus_tag="IW687_002387"
     CDS             4217..4513
                     /locus_tag="IW687_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0951627.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4537..5496)
                     /locus_tag="IW687_002388"
     CDS             complement(4537..5496)
                     /locus_tag="IW687_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0951628.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5679..6530
                     /locus_tag="IW687_002389"
     CDS             5679..6530
                     /locus_tag="IW687_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951629.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6551..6763
                     /locus_tag="IW687_002390"
     CDS             6551..6763
                     /locus_tag="IW687_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951630.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2389 a         1156 c         1296 g         2338 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
     4501 gtatatccag tagtagtttt tatttactct taattgtcaa attaagttag acaaatcatc
     4561 aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg
     4621 aatattattt cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc
     4681 catagatttc aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct
     4741 ttgagacggc gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg
     4801 tttccaattt gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg
     4861 tacagattca aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg
     4921 tttcaatgtg atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg
     4981 tttcaaacct acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata
     5041 atctttttca cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg
     5101 tccatttggt ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt
     5161 gaacatacga taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat
     5221 cacatcgggt gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg
     5281 taaaacaagc gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc
     5341 taaggctgat tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg
     5401 attcaagaaa tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat
     5461 tacaagttca tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt
     5521 tggacggaac tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta
     5581 caatcggcgt acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag
     5641 gaaaacgtta aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg
     5701 aacacacctg gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat
     5761 tatggccatt aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt
     5821 actcgattgg agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag
     5881 catcagacat ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta
     5941 ctcaattagt catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc
     6001 tattcgattt aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat
     6061 ctggaagaac aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg
     6121 gacacatgta tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga
     6181 aacttattaa acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg
     6241 gttatgagta caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc
     6301 acacgagtat ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg
     6361 aaatgaaagc aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc
     6421 tacgatatgg catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat
     6481 acattttttg gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa
     6541 tcgtttgaaa atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac
     6601 cttaaagaag gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa
     6661 aacgggtacg atgaacattg aataccgaac agaatattta gaattaacga atcaaactga
     6721 ttttttgttg attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa
     6781 ttgattgatg aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct
     6841 ttatttgtaa agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg
     6901 ctgtgcctat tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag
     6961 tgaaaggata gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc
     7021 ttttcgctat cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga
     7081 tgagaaaatc gtaagagaaa ttgatcgaag atgtgacgaa cggttctgtt gcaaagtttt
     7141 aaatctacta tcaaataagg tagaataata gaaaaagat
//
LOCUS       DACELF010000104         7153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.109, whole genome shotgun sequence.
ACCESSION   DACELF010000104 DACELF010000000
VERSION     DACELF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.109"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1368)
                     /locus_tag="IW687_002391"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="IW687_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ0951631.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="IW687_002392"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="IW687_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0951632.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="IW687_002393"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="IW687_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="IW687_002394"
     CDS             complement(2652..2882)
                     /locus_tag="IW687_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0951633.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="IW687_002395"
     CDS             complement(3112..3930)
                     /locus_tag="IW687_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0951634.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="IW687_002396"
     CDS             4091..4780
                     /locus_tag="IW687_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0951635.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="IW687_002397"
     CDS             4794..6296
                     /locus_tag="IW687_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0951636.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="IW687_002398"
     CDS             6309..6785
                     /locus_tag="IW687_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0951637.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="IW687_002399"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="IW687_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2316 a         1131 c         1295 g         2411 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DACELF010000105         7031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.110, whole genome shotgun sequence.
ACCESSION   DACELF010000105 DACELF010000000
VERSION     DACELF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.110"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            54..506
                     /locus_tag="IW687_002400"
     CDS             54..506
                     /locus_tag="IW687_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951638.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IW687_002401"
     CDS             520..1209
                     /locus_tag="IW687_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951639.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IW687_002402"
     CDS             1334..3685
                     /locus_tag="IW687_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0951640.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IW687_002403"
     CDS             3765..4160
                     /locus_tag="IW687_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951641.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IW687_002404"
     CDS             4485..5102
                     /locus_tag="IW687_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951642.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IW687_002405"
     CDS             5143..5442
                     /locus_tag="IW687_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951643.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IW687_002406"
     CDS             5476..5643
                     /locus_tag="IW687_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0951644.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6251)
                     /locus_tag="IW687_002407"
     CDS             complement(5688..6251)
                     /locus_tag="IW687_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951645.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6279..6959)
                     /locus_tag="IW687_002408"
     CDS             complement(6279..6959)
                     /locus_tag="IW687_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAQ0951646.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT         2365 a         1025 c         1385 g         2256 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt
     6241 ttgaaaatca aacgcgccct ctctgaactc caaatatctt aggctgttat tcccattaat
     6301 accttgattt cagtagacac cgaaaagccg aagagcgttc catttcttcg gttcttttta
     6361 tatattcctc gaatggtctc catgccctta atcgtggaag aggctgtacg gagactttga
     6421 taaaatttat ttcgtcgttt aataggtcga tggtcttgtt ctattaaatt gttaagatac
     6481 ttcacagttc ggtgctctgt cttagtatat aaacccacac tctgtaactt tctaaaggcg
     6541 gagccaagag aaggtgcttt atcggtcaca attgctttcg gctcaccaaa ctgtttatgg
     6601 agtcgtttta agaaagcata ggctgcttgc gtatcccgtt tctttcgtaa ccagatatct
     6661 aaggttaagc cgtccgcatc aattgcacga taaagataat gccaacgtcc cttaattttg
     6721 atataggttt cgtccatttt ccatgaatag aaggattgtc tatttttctt cttccaaaga
     6781 taatagagga ctttgctgta ctcttgcacc caacgataaa tcgtagtatg acaaacattt
     6841 attccacgat catataacaa ttcctgaact tcacgatagc ttagattgta acgcaggtag
     6901 taaccaacag cgacaataat gacgtctttt ttgaattgtt tgcctttaaa atgattcatt
     6961 actctgtcct ctctgtcttt tttctcaatt ttacactaaa atagattttt tggaaaactt
     7021 tgcaacagaa c
//
LOCUS       DACELF010000106         6630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.111, whole genome shotgun sequence.
ACCESSION   DACELF010000106 DACELF010000000
VERSION     DACELF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.111"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            810..1484
                     /locus_tag="IW687_002409"
     CDS             810..1484
                     /locus_tag="IW687_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0951647.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="IW687_002410"
     CDS             1481..1822
                     /locus_tag="IW687_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951648.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="IW687_002411"
     CDS             1852..2211
                     /locus_tag="IW687_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0951649.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2735..4816
                     /gene="cadA"
                     /locus_tag="IW687_002412"
     CDS             2735..4816
                     /gene="cadA"
                     /locus_tag="IW687_002412"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0951650.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5127..6593
                     /locus_tag="IW687_002413"
     CDS             5127..6593
                     /locus_tag="IW687_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0951651.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2195 a         1077 c         1417 g         1941 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa gaatacacac
     5041 ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca
     5101 aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg
     5161 cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc
     5221 aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt attccatacg
     5281 cattgatcgt tggacaatta gggtcagcct tcaaggatag taaaggaggc gtcagttcat
     5341 gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg
     5401 tcgtccatat tccttatttg gcacaaaagc ctcaagcaat cctgattgct ggcggttggg
     5461 cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt
     5521 cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag
     5581 tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag
     5641 ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag
     5701 tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat
     5761 ttgcagttgg aggagcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa
     5821 aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag
     5881 gctcagtcgc aatgggtatg ctgtttgcta gcgataatat tcctaaagac ttgatggcaa
     5941 atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga
     6001 tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg
     6061 atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac
     6121 gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag
     6181 gaatcattat tttgttgccg atctttggta tcaaagatat gaatgaatta gtaaaatggt
     6241 tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata
     6301 tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa
     6361 aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag
     6421 gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc caattattca
     6481 cgaatatcat cacaccaatt gtcttgatct tattaggtat gatcttgcca gcaattgctc
     6541 gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa
     6601 atacttgtta caagaggttg tgacagaagt
//
LOCUS       DACELF010000107         6363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.112, whole genome shotgun sequence.
ACCESSION   DACELF010000107 DACELF010000000
VERSION     DACELF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.112"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..1857
                     /gene="tet"
                     /locus_tag="IW687_002414"
     CDS             25..1857
                     /gene="tet"
                     /locus_tag="IW687_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAQ0951652.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSMEIIIKQKVELHPNMCVMSCTEPEQWDVVIEGNDDLLEKY
                     MSGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYM
                     ERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVME
                     GIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE
                     PYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTF
                     FTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1967..2017
                     /locus_tag="IW687_002415"
                     /pseudo
     CDS             1967..2017
                     /locus_tag="IW687_002415"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2051..3427
                     /gene="tet(L)"
                     /locus_tag="IW687_002416"
     CDS             2051..3427
                     /gene="tet(L)"
                     /locus_tag="IW687_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0951653.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            3991..5253
                     /locus_tag="IW687_002417"
     CDS             3991..5253
                     /locus_tag="IW687_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0951654.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISNLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5498..>6363
                     /locus_tag="IW687_002418"
     CDS             5498..>6363
                     /locus_tag="IW687_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0951655.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT         2203 a          913 c         1310 g         1937 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctatggaa attataatca aacagaaagt agagctgcac
      421 cctaatatgt gtgtgatgag ctgtacggaa cctgagcaat gggatgtggt aatagaagga
      481 aatgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt agaactcgaa
      541 caagaggaaa tcagaagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta tgtggattct
     1081 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     1141 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
     1201 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
     1261 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
     1321 agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata
     1381 cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa aatctgtttt
     1441 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
     1501 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     1561 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     1621 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     1681 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     1741 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     1801 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     1861 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
     1921 agatttgaat taaagtgtaa aggaggaatt aattatgaag tgaaatgaat gtaacagggt
     1981 tcaattaaaa gagggaagcg tatcattaac cctataaact acgtctgccc tcattattgg
     2041 agggtgaaat gtgaatacat cctattcaca atcgaattta cgacacaacc aaattttaat
     2101 ttggctttgc attttatctt tttttagcgt attaaatgaa atggttttga acgtctcatt
     2161 acctgatatt gcaaatgatt ttaataaacc acctgcgagt acaaactggg tgaacacagc
     2221 ctttatgtta accttttcca ttggaacagc tgtatatgga aagctatctg atcaattagg
     2281 catcaaaagg ttactcctat ttggaattat aataaattgt ttcgggtcgg taattgggtt
     2341 tgttggccat tctttctttt ccttacttat tatggctcgt tttattcaag gggctggtgc
     2401 agctgcattt ccagcactcg taatggttgt agttgcgcgc tatattccaa aggaaaatag
     2461 gggtaaagca tttggtctta ttggatcgat agtagccatg ggagaaggag tcggtccagc
     2521 gattggtgga atgatagccc attatattca ttggtcctat cttctactca ttcctatgat
     2581 aacaattatc actgttccgt ttcttatgaa attattaaag aaagaagtaa ggataaaagg
     2641 tcattttgat atcaaaggaa ttatactaat gtctgtaggc attgtatttt ttatgttgtt
     2701 tacaacatca tatagcattt cttttcttat cgttagcgtg ctgtcattcc tgatatttgt
     2761 aaaacatatc aggaaagtaa cagatccttt tgttgatccc ggattaggga aaaatatacc
     2821 ttttatgatt ggagttcttt gtgggggaat tatatttgga acagtagcag ggtttgtctc
     2881 tatggttcct tatatgatga aagatgttca ccagctaagt actgccgaaa tcggaagtgt
     2941 aattattttc cctggaacaa tgagtgtcat tattttcggc tacattggtg ggatacttgt
     3001 tgatagaaga ggtcctttat acgtgttaaa catcggagtt acatttcttt ctgttagctt
     3061 tttaactgct tcctttcttt tagaaacaac atcatggttc atgacaatta taatcgtatt
     3121 tgttttaggt gggctttcgt tcaccaaaac agttatatca acaattgttt caagtagctt
     3181 gaaacagcag gaagctggtg ctggaatgag tttgcttaac tttaccagct ttttatcaga
     3241 gggaacaggt attgcaattg taggtggttt attatccata cccttacttg atcaaaggtt
     3301 gttacctatg gaagttgatc agtcaactta tctgtatagt aatttgttat tacttttttc
     3361 aggaatcatt gtcattagtt ggctggttac cttgaatgta tataaacatt ctcaaaggga
     3421 tttctaaatc gttaagggat caactttggg agagagttca aaattgatcc tttttttata
     3481 acaggaattc aaatcttttt gttccattaa agggcgcgat tgctgaataa aagatacgag
     3541 agacctctct tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa
     3601 agacaataaa aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca
     3661 aaataaaaat tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata
     3721 ctacctgtcc cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt
     3781 ggcagagggc aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt
     3841 tttatggttt tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa
     3901 gtatagtgtg ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat
     3961 ttgtgccacc taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa
     4021 gtgaaatcag ctggactaaa aggcatgcaa tttcataatc aaagagagcg aaaaagtaga
     4081 acgaatgatg atattgacca tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa
     4141 aatattgatt acaacgaacg tgtcaaagaa attattgaat cacaaaaaac aggtacaaga
     4201 aaaacgagga aagatgctgt tcttgtaaat gagttgctag taacatctga ccgagatttt
     4261 tttgagcaac tggatccagg agaacaaaaa cgattttttg aggaaagtta taaattattt
     4321 tccgaacgat atggcaagca aaatattgct tatgcaacag ttcataatga tgagcaaacc
     4381 cctcacatgc atttaggtgt tgtgcctatg cgtgatggaa aactgcaagg aaaaaatgtg
     4441 tttaatcgtc aagaactgtt atggctacaa gataaattcc ccgagcatat gaaaaaacag
     4501 ggttttgagt tgaagcgtgg tgaacgtggc tctgaccgta aacatattga gacagctaaa
     4561 tttaaaaaac aaactttgga aaaagagatt gattttctag aaaaaaattt agcagttaaa
     4621 aaagatgaat ggactgctta tagcgataaa gttaaatcag atttagaagt accagcgaaa
     4681 cgacacatga aaagtgttga agtgccaacg ggtgaaaagt ccatgtttgg tttgggaaaa
     4741 gaaataatga aaacagaaaa gaaaccaacc aaaaatgttg ttatatcgga gcgtgattat
     4801 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
     4861 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
     4921 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
     4981 cttgaagaaa acaagtcttt aaagtctaaa ataagcaatt taaagcgtga tgtgagttta
     5041 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
     5101 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
     5161 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
     5221 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
     5281 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
     5341 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
     5401 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgaccaa
     5461 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
     5521 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
     5581 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
     5641 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
     5701 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
     5761 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
     5821 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
     5881 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
     5941 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
     6001 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
     6061 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
     6121 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
     6181 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
     6241 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
     6301 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
     6361 tga
//
LOCUS       DACELF010000108         6237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.113, whole genome shotgun sequence.
ACCESSION   DACELF010000108 DACELF010000000
VERSION     DACELF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.113"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..637)
                     /locus_tag="IW687_002419"
     CDS             complement(23..637)
                     /locus_tag="IW687_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951656.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="IW687_002420"
     CDS             951..1373
                     /locus_tag="IW687_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951657.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="IW687_002421"
     CDS             1383..1586
                     /locus_tag="IW687_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951658.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="IW687_002422"
     CDS             1797..2381
                     /locus_tag="IW687_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951659.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..2618
                     /locus_tag="IW687_002423"
                     /pseudo
     CDS             2550..2618
                     /locus_tag="IW687_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3323..5185
                     /gene="ltrA"
                     /locus_tag="IW687_002424"
     CDS             3323..5185
                     /gene="ltrA"
                     /locus_tag="IW687_002424"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0951660.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESRDDHIFNNLMEIVTS
                     PNNILLAYRNIKGNSGSVSPGVDNKNIDDLKEIPNADFIRIVQGKFSEYKPQPVKRVE
                     IPKPNGKNRPLGIPTIWDRIVQQCLLQVLEPIMEAKLHDKNYGFRPNRSAHHALAQAV
                     RLAQLSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGIQDRWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDCYQANQGYWNKG
                     NKYRALRRSKLKEIYIVRYADDFKIFCRRRSDADNIFIATKLWLKERLKLDISLEKSK
                     VVNLKKKKSEFLGFTMKLVRKRKTFVIETHMCPKAMKKVSDNLTKQIKVIQHTPTKED
                     LQKQLVKYNSMVIGIHQYYRRATHISVDCPKINQRIRIIIKNRLDVKKNGSPPSDFVK
                     KNYARSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSAQRGRCRVTKELLELEDIYCHRIIPKNLGGKDNFQ
                     NLVIIHQNIHQLIHENDRTRASLLITRMRITGAQLEIINQFRKKAQKCELSLNL"
     gene            5267..>6237
                     /locus_tag="IW687_002425"
     CDS             5267..>6237
                     /locus_tag="IW687_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0951661.1"
                     /translation="MEIISKDYLSLSSVECIERNLDPLTTELVVMSRSDNTGSGLILA
                     SSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFRRYVADEDL
                     LIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIATVGVGDNPLL
                     AKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGILSIRD
                     LANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRN
                     EIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMK"
BASE COUNT         2142 a         1054 c         1227 g         1814 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acggtgcgaa acgttcctaa ctgaaaaggt
     2641 taggcacaca agactttctt gtgtagaact aatgatttga ggagtgaaat gacggtgtaa
     2701 cgacctgaag cgctccctct gatactccga ttggcaatac ctttagttag aataggaatt
     2761 gtcaaaagct cggtaaagtc ggctgagagt taccgtagtt gcattatgca atcgaaagct
     2821 gttcagcggt gaatggtgta acctataggt cgggagtcca gaaattactc atggtgagaa
     2881 tgtgcagttg cactgacgaa ctcgcgaatg tacgaatcta taagcttggg gtgcagaaat
     2941 gtatcttacc cgcacaaata ttactgtgac aaagggtgta gcttgcggtt aagtaagaat
     3001 cgtagatatg aacaaccgtg caatgtggta gacattggaa agttacaggt tcagagtgag
     3061 cacctaaaag taaatgcagg gataaaatca ttggaacgtt gaaagctagt gtcgtggagg
     3121 actaacttcc tttgaaacgg ttgagacgaa gaataataag ctcatttaca ctagtgaaag
     3181 tagtggcaca gtacctatga agcagtgata ataaactgta gagggatagc cacaagtcaa
     3241 tttactctac taacagtaat gatataatcc atagaatcga gtatgacaaa ataaacgccg
     3301 acgtccgaaa ggaggcggtg ctatggaaaa cgtatcagct tcacattcaa tccgtcatat
     3361 ggaatactat ggcatgattg acagctttga taatctatac aaagaaagtc gcgatgatca
     3421 tattttcaat aaccttatgg aaattgtaac ctcgccaaat aatatcttac tagcgtatag
     3481 aaatattaaa ggaaattctg ggagtgtatc cccaggcgtt gataacaaaa atattgatga
     3541 tttaaaagaa atacctaatg cggattttat cagaatcgtg caaggcaaat tttcagaata
     3601 taaaccacaa ccagttaaaa gagttgaaat cccaaagcca aacgggaaaa atcgcccact
     3661 cggcatccca actatttggg atagaatcgt acaacagtgt ctactacaag tcctagaacc
     3721 cattatggaa gcaaaattac atgataaaaa ttatggtttt cgtcctaacc ggtcagcaca
     3781 ccatgcactt gctcaagcag tacgcttagc acaattgtct aaacttactt ttgtagtaga
     3841 tatagatatt gagggattct ttgataatgt aaaccattca aaactcatta aacagctatg
     3901 gacattagga attcaggaca gatggctttt aggtgttatt agggcaatgc ttaaagcacc
     3961 gatcatccat aaggatgggc gaatcgaaca ccctaagaaa ggaactccgc agggcggaat
     4021 tctttcccct cttctcgcaa atgttgtgtt aaatgaatta gactggtgga tatcatcaca
     4081 gtgggaaacc caccccacaa gacataatta cgattgctat caagctaatc aggggtattg
     4141 gaataagggt aacaaatatc gagcgttacg tagatcaaag ttaaaagaga tctatattgt
     4201 gagatacgcc gatgacttca aaattttctg tagaagacgt agcgatgcgg acaatatatt
     4261 tatagctacc aaattatggc taaaggagcg cctgaagctc gatattagcc tagaaaaatc
     4321 aaaagtggtt aatctgaaga aaaagaaatc cgaattcctt ggtttcacta tgaaattggt
     4381 tcgtaaaaga aagacctttg tcatagaaac tcacatgtgc cctaaagcta tgaaaaaagt
     4441 atctgataat ctaacaaaac aaataaaggt aattcagcac acccctacca aagaggattt
     4501 acaaaaacaa ctagtgaaat acaactcgat ggtgataggg attcatcaat actatagaag
     4561 ggctacccac attagtgtgg attgcccaaa aattaaccaa cgtataagga ttatcatcaa
     4621 aaatcgattg gacgttaaga aaaacggatc accgcctagt gatttcgtta aaaaaaacta
     4681 tgcacgttca aaacagttaa gatggattaa tggctatcct atgattcctc taggattcgt
     4741 taaacacagc catacatcgt tacctaaaat ggggatttgt aagtacacgc ccgaaggacg
     4801 tgtgctaata cacaaatatc ttaccttccc aatcgatatt atcaaccaac ttatgaatat
     4861 gaataacccg gatgaaagca ttgaatttag ggataatcgt gtctctaagt actctgcaca
     4921 acgaggcagg tgccgagtaa caaaagagct cttggagcta gaagatattt attgtcaccg
     4981 tatcataccc aaaaatcttg gtggaaaaga taacttccaa aatctagtta tcattcatca
     5041 aaacattcat caacttattc atgagaatga tagaacgaga gcttcactcc tcataacgag
     5101 gatgaggatt acaggcgctc aacttgagat aatcaaccaa ttccggaaaa aagctcagaa
     5161 gtgtgaattg tcgctgaatc tataactgta tgataattgt tagtaaacat tgatggaacg
     5221 ccgtatgcgg tgaaagtcgc acgtacggtg tggagcgggg gaaaatctgg agattatatc
     5281 aaaggattac ctatcgctat caagtgtcga atgtatagaa agaaatttag atccattaac
     5341 aacagaactt gttgttatga gtcgatctga taatactggt tcaggattga tattagcttc
     5401 ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg agtagaccac gtgacttacc
     5461 acaaccattt cctaaaacac tacacgttgt tccaccacgt atgaacctat atatcaagcg
     5521 aaatatgcag gtaaataata ttttcagaag atatgtggct gatgaagatc tactgattta
     5581 ctcgatcgat gaatcgattt taaaagtgac aaaatcactg aatcttttta cgactgaaga
     5641 aacacgaagt cagcgaagga agaagctcgc tcaaatgatc caagaacgta ttaaagaaga
     5701 gttaggtttg attgctacgg taggcgtcgg cgataatccc cttttggcta aattagcctt
     5761 agacaatgaa gcaaagcata atgaaggatt tattgcggaa tggacgtacg aaaacgttcc
     5821 tgagaaagta tggaatattc ccgaaatgac ggacttttgg ggaattggtt cgcgaatgaa
     5881 aaaaaggctc aaccaaatgg gaattttgag cattagagac cttgctaact ggaatcctta
     5941 tacgattaag aatagattag gtgtgattgg attacagctg tacttccatg ctaatgggat
     6001 cgatcgaaca gatatagcaa tcccaccaga accgacaaag gaaaaatcat atggaaattc
     6061 acaagtgtta ccacgagatt atactagaag aaatgaaatc gagctggtgg taaaagaaat
     6121 ggcggaacaa gtagcgatac gcattaggca gcataactgt aaaacaggtt gtgtccattt
     6181 acacattggc acgtcgatct tagaaacgag accagggttt tcacaccaga tgaagat
//
LOCUS       DACELF010000109         6222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.114, whole genome shotgun sequence.
ACCESSION   DACELF010000109 DACELF010000000
VERSION     DACELF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.114"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            221..655
                     /locus_tag="IW687_002426"
     CDS             221..655
                     /locus_tag="IW687_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0951662.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IW687_002427"
     CDS             678..998
                     /locus_tag="IW687_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951663.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IW687_002428"
     CDS             1139..1330
                     /locus_tag="IW687_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951664.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IW687_002429"
     CDS             complement(1401..1658)
                     /locus_tag="IW687_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0951665.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IW687_002430"
     CDS             1816..2079
                     /locus_tag="IW687_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951666.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IW687_002431"
     CDS             2103..2291
                     /locus_tag="IW687_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951667.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="IW687_002432"
     CDS             complement(2374..2559)
                     /locus_tag="IW687_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951668.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="IW687_002433"
     CDS             2774..3145
                     /locus_tag="IW687_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0951669.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="IW687_002434"
     tRNA            complement(3295..3367)
                     /locus_tag="IW687_002434"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="IW687_002435"
     CDS             complement(3413..3841)
                     /locus_tag="IW687_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951670.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="IW687_002436"
     CDS             complement(3901..4659)
                     /locus_tag="IW687_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0951671.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4954..5151
                     /locus_tag="IW687_002437"
     CDS             4954..5151
                     /locus_tag="IW687_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951672.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5257..5739
                     /locus_tag="IW687_002438"
     CDS             5257..5739
                     /locus_tag="IW687_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951673.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(5797..>6222)
                     /locus_tag="IW687_002439"
     CDS             complement(5797..>6222)
                     /locus_tag="IW687_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0951674.1"
                     /translation="HEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDT
                     DVVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAK
                     EWNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2111 a          964 c         1228 g         1919 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     4801 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     4861 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     4921 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     4981 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     5041 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     5101 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     5161 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     5221 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     5281 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     5341 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     5401 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     5461 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     5521 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     5581 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     5641 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     5701 gatgaggcag agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat
     5761 gtttgtcaca acctctgttt tttatttcaa ataaaattat ccaaatacgc ggaagcgatc
     5821 ggcatcgttc atgtattctt tttccccagc cggtgtttct ggtgaaccga atactaattg
     5881 cgaacgtagt ttccagtttg aagggatatt ccattctttt gcaactgctt cgtcaataac
     5941 tgggttgtaa tgttgcaagt ttgcgcccaa tcctttatca actaatgcta cccaagtatt
     6001 agctgttgcg attccgtttg attgttctga ccaatctggg aagttgtctg catataattc
     6061 aaattgttct tgtagacctt ttaccacgtc agtatctttg aagaataata ctgtaccata
     6121 accatttttg aaaccagcta atttgttttg tgtatttggg aaagcttctg ctggagtcaa
     6181 tgggcgaagt gcttcttcag tgatttccca aagtttttca tg
//
LOCUS       DACELF010000110         6172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.115, whole genome shotgun sequence.
ACCESSION   DACELF010000110 DACELF010000000
VERSION     DACELF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.115"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            725..811
                     /locus_tag="IW687_002440"
     CDS             725..811
                     /locus_tag="IW687_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0951675.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IW687_002441"
     CDS             complement(932..1621)
                     /locus_tag="IW687_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951676.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IW687_002442"
     CDS             complement(1628..1906)
                     /locus_tag="IW687_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951677.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IW687_002443"
     CDS             complement(1866..2780)
                     /locus_tag="IW687_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0951678.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IW687_002444"
     CDS             complement(2762..3142)
                     /locus_tag="IW687_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0951679.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3591..3878)
                     /locus_tag="IW687_002445"
     CDS             complement(3591..3878)
                     /locus_tag="IW687_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0951680.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3898..4122)
                     /locus_tag="IW687_002446"
     CDS             complement(3898..4122)
                     /locus_tag="IW687_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0951681.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4762..5295)
                     /locus_tag="IW687_002447"
     CDS             complement(4762..5295)
                     /locus_tag="IW687_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0951682.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5316..6158)
                     /locus_tag="IW687_002448"
     CDS             complement(5316..6158)
                     /locus_tag="IW687_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0951683.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1151 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc ccactctcca caatacaaaa
     3361 aagactagca aatgctagcc actttttgta aagtttcgct tgtttcacct tcggtgaaat
     3421 tgttgcaaat caataattag gtagagctac atttttttcg ttgttcaaag attcttatgc
     3481 catttactat taataaatct ataaatatga taatttttcg gctctataat ttatgggact
     3541 gatgaatcag aattgattct tcagtcccat tttcatttta ttccaaagaa ttaactccaa
     3601 ataccaatag aagcccatcc tctatatact tgagattcct tactaacaat ttcttgtaat
     3661 tctattaaag atttaggtaa tccaaattta tgttttaaaa tatatcttcc aagaaaacta
     3721 cttaatttca tactaacttc accaacactt tttccctcac tgatttcttt agaataaaga
     3781 aagagctttt cttttaagtc aggatagtca tctatagttg cttcattgta tactttacta
     3841 attgcattta aaagtttttc ttctttagta aaatccatta taataacctc cctaccacta
     3901 ccttctaggt gcccatggac catgattaac ccaaccatta acaataattt ttccaatttc
     3961 tcctttagct tgattccaat ctacccaaca tttttctttg ttacaataaa gaccatttcc
     4021 atagtaagta gctgcatcaa cttttattcc tgtaccgatg ccagctagac aagttcctaa
     4081 tattcctaga ataacacaat gttttaatac tttctttttc atgattgttt ttccttttcg
     4141 tttttttaaa attgcttgtt atattaaata atcttagtaa ttttattata taacatttta
     4201 ttatataaca atgattaacg tcataaaaag tagttttgaa ttcataatcg tttaaataaa
     4261 attataagat cagttattta aacagctagt agacataatg tacatactac caagtaacgc
     4321 ctgtaccaag ggatccaggc gctgattttg tgaattttta ttggtctagg ttaaaagctc
     4381 tttttaaggc ttggttttct gcggttgttt ctttctaatt ctttgtgaat attgatcggt
     4441 cattttttaa atacttgtta tttttgtttg tgtttgttat gctttttaca tatctcatat
     4501 tcatttatta ttcttttcta aggttaataa taggataatc tctttcagaa agactgctcg
     4561 tacgagcagt cttttcttat ttatcaagtt tttctacaaa tacttcctca ttttgactgt
     4621 tgtagtaatt aggattgagt tttagttccc cttttttatt tcctttgttg gccactgata
     4681 ataacattgg gctaaagctt aatattccta gtaggataga accgaatagc aagtgttttt
     4741 ttgtcataag tatttccttc ttcatttcca tagctgccac cattttttcc aattctttgt
     4801 ttgttcgtcc attgtttctg ttgcttcaga aagagtcgtt gaactattct ctgaagactc
     4861 ttccgtataa gtcaactgca gttgctcctg aagtttttcg atttgttggt tggcttgcag
     4921 ggtgagttgt tgttgctggt cgagtagttt ttgtatatga gaattatttt gctctttgag
     4981 ggcaagttgt tctcgtaaaa attcattttc ttcacgcaac tgatctagca acttgtttgc
     5041 aaccacgttt ttgtttggta tataagcatt tttatcgttg ttttgttcat gtttatgcaa
     5101 cttatctagc aacctttgat tgtctaagtt gcgagcaagt tgcaatactc cagaacgcaa
     5161 tacaacctta taaccacgct catttttagc tatcatggaa gaatctaatt tttggatatg
     5221 tttcgatatt gcttgcctac tcatcttaaa aagatctgac aattccttta ttgtaaaggt
     5281 ttcttgtggt tgcatactgg ttgcctccta ggttgctact ggaataaatt atctaaatca
     5341 gataataaac tttttttctc tttttccgaa agtgcggact ctgtttgttt ttcaagagct
     5401 tttttctctt tttctagttg tttgatttgt tggaggagtt caagttctcg tttcttctgt
     5461 ttgatttttt cgttaattgt tgtagcgggt tcttcgtatt ctttttgttt atcagctaaa
     5521 gccaatgctt tattttggtc ttctttggtt ggtgcatgtt tgttaaagtc gtctgctttt
     5581 ttgatctcag gtttgaacgt aaattgatag ccaataacag gttttccccg tccttttccg
     5641 tatttctttt tgattgttag tcctttgaag attgcagtta gttcttgacg tataggtttt
     5701 ataactcgtt tatctatatc aggttgctta taacttttag gaatatctaa taattcaaga
     5761 aagtcttctt tggagaaata tgcgaaacct ttggttctgt attgctttaa cagacgaaac
     5821 atagtctttg catagccact ttgtagctcg ttgaattgtt gtaaagaata gcgtacccac
     5881 tcgtctaaat tatttaacaa aggcagtgct ttttcgtaaa ttcgtatatc tacgtaagga
     5941 ttttctacgt ccccgttgat tttaaattct gtaaacatta caaacatatc gtaagaaagc
     6001 ccagactcac ttctacgacc aaagcgtaaa gataagattt tttcataagt tgcttttaaa
     6061 tcttctacaa atcgtacatt tgcagttgct ttgtagttac ttaaatcttt tagttggtca
     6121 aacgtaaaac gaactgtctt atcccctttt tcacgcattc tagacacgat gg
//
LOCUS       DACELF010000111         6136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.116, whole genome shotgun sequence.
ACCESSION   DACELF010000111 DACELF010000000
VERSION     DACELF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.116"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW687_002449"
     CDS             complement(207..1169)
                     /locus_tag="IW687_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0951684.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW687_002450"
     CDS             complement(1171..2610)
                     /locus_tag="IW687_002450"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0951685.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW687_002451"
     CDS             2795..4741
                     /locus_tag="IW687_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0951686.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IW687_002452"
     CDS             5005..5877
                     /locus_tag="IW687_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0951687.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1947 a         1055 c         1083 g         2051 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttcttc
//
LOCUS       DACELF010000112         5974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.117, whole genome shotgun sequence.
ACCESSION   DACELF010000112 DACELF010000000
VERSION     DACELF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.117"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            738..1472
                     /locus_tag="IW687_002453"
     CDS             738..1472
                     /locus_tag="IW687_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0951688.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1488..2657
                     /locus_tag="IW687_002454"
     CDS             1488..2657
                     /locus_tag="IW687_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0951689.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2673..3668
                     /gene="lacD"
                     /locus_tag="IW687_002455"
     CDS             2673..3668
                     /gene="lacD"
                     /locus_tag="IW687_002455"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0951690.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3747..4673
                     /locus_tag="IW687_002456"
     CDS             3747..4673
                     /locus_tag="IW687_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0951691.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4692..5942
                     /locus_tag="IW687_002457"
     CDS             4692..5942
                     /locus_tag="IW687_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0951692.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2003 a          974 c         1223 g         1774 t
ORIGIN      
        1 atccgaggag gctcattgtc aaggacaatc ggaatagcat acggaaagaa gttctgcacc
       61 ttataaaatt tgtcaaaaaa actgttgcca ttccaaagtt tgaaaaatgc tatttcgagg
      121 tagaaggata ttatataagc acggttgact aaacgaagtg ataataaaaa aagttattca
      181 agaataagaa aaattatgat acgaattcag ataaactgga gaatacagag acctttaaaa
      241 gggataagaa acaacataaa ttcctcttaa gagagaacga aaggacaaag ccagcaactt
      301 gatacacgag ctagtattag aggaatttat tctcatagac tcccttttga atacgtttca
      361 actttaattt tcatgttcat aaatgatttg aaaccaaagc aaatgcgttg cacttttcct
      421 ccgtcttccg aaaatcgttc aattcactgg taagtagtca tttgatagtt tgaggcaaat
      481 tgttgattga tgaagggtag gagctaaaaa aagataccag ttgaagagag aaaaagaaaa
      541 aaatagaggg tgttcttgaa tgaaaaaagt tggaaaatta tttttcattt ccttttattc
      601 ctattaaagt cagtgatgtc aatcatatta acaagactaa aaatgaaaat aaaaaaaaga
      661 ttgttcttag tattgactca taaaaaatac cactactata cttacattgt ggttatagcc
      721 agttaggaga gacatcgatg caaaatacct ttcaatcaaa atcactttat tatcagctgg
      781 tagaaatatt gaaacaacga atcgaaaaca gtatggtcgc tcatgacaag ctgccatcag
      841 aaagagagtt gaccagtttt tatggcgtta gtcgcacgac ggtgcgacta gcattgcaag
      901 aactggaaaa aatgggctac atttatcgac aacatggaaa aggaaccttt gtttcggaat
      961 taaggacaaa tgcgactgat ctagctggcg catatagttt taccgaacaa atgcgggcaa
     1021 tgggcaaaca tcctgaaacc aagatattgg ccttcaagaa gattgaagca actaaatatt
     1081 tagcgagtca tctgaatgtt caactaggtg agcctttatt tcaattagac cgattgcgct
     1141 tagctgatga tttgccaatg atgatcgaga aatcctattt gccagtgaag ctatttatgt
     1201 ccctttctca agaaattcta tttctgaaac ctttatataa tttgttttca gagaatttca
     1261 atcaaatcgt acggttggct gacgaggaat tttatgccag cattgtccag aaagaagata
     1321 gtgagttgct aaatattcca gaaggatcac cagtgctcca tttagttcgc tcaacctata
     1381 atctcgagaa cgaagtgatt gaatttacat taagtgtagc gcgggctgat aaatttcatt
     1441 ataagatcag gcatctgcgt tccactaact gaattggagg aaagaaaatg tttatgttct
     1501 caaaagaaga actacgaaag ctaggagcag aaaatactac ccgagaaatt cgacagcaac
     1561 cggaactgtg gcatgagacg tttgaccgtt atcaagaaac agttgcagat ttagatcgtt
     1621 tgtttacgga tgcgaaacaa catgctggag agcgaccaat ccgagtcatt tttacagggg
     1681 ccggaacctc gcaatatgtg ggagatacac taattccgtt tcttactagt caaggaaata
     1741 cgaaccagtt tatttttcaa agtattggtt cgaccgatat cgttgctacg ccaaaagaat
     1801 acctgattcc tgatgagaca acgatcttgg tctcatttgc ccgtagtgga aatagtccag
     1861 aaagtctagc cgcagttaag atggccaatc agctggtcaa caagatctat catatcgtga
     1921 tcacctgtgc accagacgga ttattggcta cacaaatgcc tacaaaaaaa gaaaatctag
     1981 ttttactaat gccaaacaaa gccaatgacg ctggttttgc tatgacaagc agtttcagct
     2041 gtatgacgct gactgcgcta cttatttttg atcaagcaag caatcagcaa aaagaaagat
     2101 atgttcatca attaagccga ctaggagaag aagtcatcca acgagaatca gagatccaag
     2161 ggtatctggc atcagacttt catcgcatca tttacttagg ttcgggaagc ttaggcggat
     2221 tgactagaga agcgcaatta aaagttctcg agttaactgc tggaaaaatc gcaaccgtat
     2281 ttgatacatc tttaggattc agacatggtc ccaaatcttt tgtcaatgag caaacattgg
     2341 ttttcctgtt tgtcagcaat gactcatata cgcagaaata cgatgtagac atgctggaag
     2401 aaatcaaaaa tgacggtatt gcccaacgcg ttgttggaat tggtcaaaag ggttcattgt
     2461 ttagtggtga ttcctttgaa ttagttgcag attcgttact gccggatggc tatcttgctt
     2521 tagcagatat catggtagcc caaacgatcg cattactttg ttcggtgaag gttggtaata
     2581 caccagatac accatcgcca agtggaacag tcaatcgtgt ggtcaaaggc gtaacgattt
     2641 atccatatga aaaataggaa agagtgtgaa gcatgttaga atggtcggaa aataaaagac
     2701 aagcaatgga acgtctatcg acgccagaag gcattattac cgctttggcg atcgatcagc
     2761 gaggcgcgct gaagaaaatg atgtcggctt tagggatgga agcgacaaag gaaaaaattg
     2821 agcagtttaa aacactaatt tcccaagaat tgactcctta tgcctcagca attttattgg
     2881 atcctgaata cggactgcct gctgcacaga agcgtgcagc agcgagcggt ctgctgttgg
     2941 catatgaaaa aactggttat gatcaaaccg tacccggccg cttgccggat ctgctgtcgg
     3001 actgttcagt tctgcgattg aaagagttag gtgcggatgc ggtgaaattt ttactgtatt
     3061 acgattatga agaagcagag gaaatcaatc ggcaaaagca agtttttgtt gaacgaattg
     3121 gaagcgaatg catggctgaa ggactgccat tttatttaga gttattgtca tatgatgcta
     3181 aaaatgtaga tacgaaatcg ctttcctatg ccaaagcaaa accgcgcaaa gtgatcgaaa
     3241 tgatgaaaga attctcgaaa acccagtata aagtagatgt cttaaaggtt gaagtaccag
     3301 taaacatgtc atttgttgaa ggaaatgcaa caggggagat cgcctataca cgtcaagaag
     3361 ctgccgctta ttttaaagaa caaagcgatg caacagattt gccgtatatc ttcttaagtg
     3421 ctggggtatc agcggcatta ttcatggaga cattgcgttt tgccaaacat gctggggcac
     3481 gattcaacgg tgtactctgt ggcagagcaa cttggaaaga tgcagtcgct cctttcgttg
     3541 aaaaaggaga agcggcaact ttagaatggc tgacagaaca agggacacaa aacattcgtc
     3601 aattgaatga agtgataaga gaaactgcca tcccatggta tgagaaagta agtgaaagca
     3661 cttgttaatg tttctaatat attagtagtg ataagagata tgtgtgatta taatttatca
     3721 ttattgaaac tgggaggata ttttatatga ataaaagatt gttagattat tcagcttcag
     3781 attttttgaa agctaagccg atggaattaa agcaggcaat tttggcttct gaaggtagaa
     3841 caattatggc agaaaatgtg ccaagtagct gtccttacat gggaaaatca gtgacaaatg
     3901 cagaattgga gagagctgct ggagctgatt taattttgtt taatgcacta gatcttttta
     3961 atcctcaaat ttcaggtgta cctttaactc taaaagagaa tccaataatc tggattaaaa
     4021 aagctttagg aagagctatt ggtgtaaatt tagaacctgt agacaaagaa gttttgatga
     4081 gtgaaaatcg aactgaaatt tcaagtggta gaatcgtttc tcctgagact ttaaaaaagg
     4141 caaatgaatt aggctttaat tttatttgtt taactggaaa tccaggaaca ggagtatcta
     4201 atagaactat tgagaaagca ataacattat ccaaatcaat ttttaatgga ttaattatcg
     4261 ctggaaaaat gcatggtgct ggtgttgatg aaccaattat gaacttagat atagcaaaaa
     4321 gatttattaa aacaggtgta gatattttat tagttcctgc tccatacact gttccgtatt
     4381 tcatggaaag tgatttgcga ggaatcgttg attatataaa agattttaat agaaataaat
     4441 ctattgagga aaaagttcta gtactaacgg ctaatggaac aagccaagac tcttctgacc
     4501 aagaaactat taaaaaaatt gcattagcat caaaagcatg tggagctgat attcaacaca
     4561 taggggattc tttaaatggt atttgtcttc cagaaaatat ttttgctttg ggtcaagcaa
     4621 ttcggggata tcgtcatcaa attattatgt taggaaaatc taattatcgt taattaagga
     4681 ggaaatgaaa gatgatcgac aaagttcaaa aaatagctgc aaaaattagt aatcaaagac
     4741 atctactagc tttaagagac agcatggctg cagttatgcc attaatcatt attggttcct
     4801 tttttatgtt attagcaaat ttcccgatag aaggatttac aaaatggtta tctgaaattg
     4861 gagtacaatc ctttttgaac aaagcatcag attcaacatt tggtattatt ggattagctg
     4921 ttacctactc tttgggacac aatctagcaa agacttatgg tgtagactca ttatcagcag
     4981 gattattatc tttgagtgga tatgttttac ttacaccttt attggatgca gatggttcta
     5041 taggatttcc aacaaaatac tttggtacct cgggtctttt tgttggtatt attgtagcac
     5101 ttgtatctac agaaattttc cggtggtttg tacttaaaaa tattacaatt aaaatgccgg
     5161 caagcgttcc accaaatgta agccgagctt tcgttgctat tattcctggc ttttttgtcg
     5221 ttctactatg gtttattgtt gttgtaattt gttataaact tggaattgaa aatgttcatg
     5281 ctcttattgc agatacactc gcaaaaccac tatctttact gacgaaaact ttaccaggaa
     5341 ttatattggt tatacttatt caatgtttct tttggatgtt tggtattcat ggtgcacagg
     5401 taacggggcc aataattgaa ccgttattat tgcaaaactc tgatgtaaat agaattgctt
     5461 atcaagctgg aaaagaactt cctaacatta taacttatga attcttatat aactttgttt
     5521 tttctggtgg tgcaggttgt ttatttgcac ttgccatttt aacatttttc ttttcaaaga
     5581 gtcaggaaaa taaaacttta ggtaagttat caatcgctcc agtaagtttt caagtagcag
     5641 agccccttct atttgggttt ccaactatat taaatataaa aatgttaatt ccatttgtaa
     5701 ctgcaccagt agtaacaaca ttaattactt atttttcaat gagtatggga ttagttgcaa
     5761 aacctgtagg agcaacaatc ccatggacaa ctccaccgat aattgccgga tttttagctt
     5821 ctggtggacg aataagtggt gcagtgattc aagtaataac aattgcaata aatgttttga
     5881 tatactatcc attctttaaa ttagatgata atgccaagct aaaaagcgaa aaaaatgatt
     5941 agaactacct atatgtttct aactgtagtt aagt
//
LOCUS       DACELF010000113         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.118, whole genome shotgun sequence.
ACCESSION   DACELF010000113 DACELF010000000
VERSION     DACELF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.118"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..1170)
                     /locus_tag="IW687_002458"
     CDS             complement(76..1170)
                     /locus_tag="IW687_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0951693.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IW687_002459"
     CDS             1359..2120
                     /locus_tag="IW687_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0951694.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW687_002460"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW687_002460"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0951695.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IW687_002461"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IW687_002461"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0951696.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IW687_002462"
     CDS             complement(3760..4773)
                     /locus_tag="IW687_002462"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0951697.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IW687_002463"
     CDS             4938..5858
                     /locus_tag="IW687_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0951698.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACELF010000114         5909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.119, whole genome shotgun sequence.
ACCESSION   DACELF010000114 DACELF010000000
VERSION     DACELF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.119"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2015)
                     /gene="copB"
                     /locus_tag="IW687_002464"
     CDS             complement(<1..2015)
                     /gene="copB"
                     /locus_tag="IW687_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0951699.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNL"
     gene            complement(2035..4221)
                     /locus_tag="IW687_002465"
     CDS             complement(2035..4221)
                     /locus_tag="IW687_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0951700.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4221..4430)
                     /gene="copZ"
                     /locus_tag="IW687_002466"
     CDS             complement(4221..4430)
                     /gene="copZ"
                     /locus_tag="IW687_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0951701.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4443..4883)
                     /locus_tag="IW687_002467"
     CDS             complement(4443..4883)
                     /locus_tag="IW687_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0951702.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(4957..5496)
                     /locus_tag="IW687_002468"
     CDS             complement(4957..5496)
                     /locus_tag="IW687_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0951703.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1525 a         1328 c         1040 g         2016 t
ORIGIN      
        1 aggttctgga tcattttctt tcgcgtttgc ttggctagat caagaaaatg caaaatatct
       61 tttggatcgc tgtttgtcaa tacgacatcc gcagaatcga ttgcgatatc tgtccctgca
      121 ccgattgcca tcccgattga agcacgggct aaacttggcg catcattgat cccatcaccc
      181 accatgatta cttttttccc ttggtcaagg tatttctgga caacagcttc tttatcatct
      241 ggtaataaac cgccataata ttcttcgatc cctaaataat ctgctactgc ttgggcagcg
      301 tttttattgt caccagtaag catgacagga gtgatttttc tttctttcaa tgttcggata
      361 aattcttttg cttctgtttt tactacgtca cctaaagcaa cgattgcagc tagatgattt
      421 tctaaaatca ggaagctgat ggtatttcct tgttcttggt aaggtttcaa taatgcttgt
      481 tctacgttta atccgagacg ctcaacttct ttttcattaa ctattttcac ttgttgtttt
      541 ttgactgttg cctctagccc gactcctgaa agatttttta gattttcagc ttgataaggc
      601 ttgatttcat gttctttcaa ataattcata atgccaacag ccaatggatg attggcattc
      661 gcttccaagg ctcctagata ttgaaggatt tcatttttat tgaacgcatc atcaagtact
      721 tcgacacccg ttacagtaaa ttttccttct gttagtgttc ctgttttgtc cagcatgatc
      781 acatctagat cattggcttg ctccagtgca ttgcggtttt tcaaaagtaa tccgttttta
      841 gctgctattg atgtggagcg agcaacaact aatgggatag ctaaacctag agcatgagga
      901 caggcgatga taaagactgt taccatacgt tctaatgcat taggaagatc tgtcaagaac
      961 aaccaggcga tgaatgccag gatacccaca attaatgcga cataaaacag ccattttgcc
     1021 actttgtcag ataatgactc tagcttggat ttttctcctt gtgcttttcg caccatttcc
     1081 ataaccttcg ctagataacc attttcacca gttccagtta cttcgatttc gatcgttcca
     1141 tccccattga tagaaccgcc aatcactttg tcattggctt gttttttgat tcctcttgat
     1201 tcgccagtca cagctgattc gtcaacaatt gtttcacctt tcaagattac accatctgtt
     1261 ggcattttat caccagaccg gacgatcaaa cgatcacctt cgtttacttc tttcaatgaa
     1321 actgtctctt cttttccatc tttcgtcaag cgtttcactg attctggcag taattctgct
     1381 agtttttgta aagcattgct ggcattcgac acagcattca tttcaatcca gtgacctagt
     1441 agcataataa cgatcaaagt agccaattcc cagaagaaat ccatgacgtg tgtatgtgga
     1501 ttgagcagat ttgcgataaa cgaatagaca ctataaatat atgcaactgt aatccccatc
     1561 gcgatcagtg tcatcatagc aggacttttt tgtttcaatt ccatcttagc tccacttaaa
     1621 aatggctgac cgccatagat aaatagtata gttgctaaga tcaatacgag ccaatcagaa
     1681 ccaggaaacg ttacttgaaa tggaaattcc attcccatca ttggtgaaaa aagaatgatt
     1741 gggatagcta ggataagcga tagccaaaat ttttgtttga aattccccat gtgcatggaa
     1801 tgatccatgc cgccgtgttc gtgatccatg tgcatcgaat ggtccatttg tttgctgttt
     1861 gctatatctc catggttatg tcccatgtgc atggaatgat ccatctgact atggtcatgt
     1921 ttcatctcca tctgtttttc atgagattct gtactttctt tgtttactac ttctttttta
     1981 tgctcatgtt ttttctccat atcgttccca ttcattttta ctccaaccct tctcttactt
     2041 tttcatgtgt cgatttaagc tcagggagtt caacaataca ctgacggaac taaacgccat
     2101 cgctcctcct gcaatgattg gattcaaaaa gcctagtgcg gcaaaaggaa taccaatcgt
     2161 attatagaca aaagcccaga aaagattttg tttgattttt ctcaatgtca acctagacaa
     2221 ctcgattgtt tgtgcaatgg atgccaaact gctattcatc agcgtcacat cggctgtttc
     2281 catcgcaatg tccgttccac ttcccattgc cataccgaca tcagctaatg ctaaggccgg
     2341 tgcatcattg attccatcgc ctgccatacc gacttttttg ccatctttct gaagtttttc
     2401 tacgtaggct gctttatcct ctggaagtac ttcagcaaag atatgctttg gatcaatacc
     2461 aacttgttta ccaatcgttt cggctgccag tttgttgtct cctgtcagca taaaaacatc
     2521 taatccttta ttctggagct gcttgattgc ttgtttggct tctagtttga tttgatcagc
     2581 cacggcaatc cacccgatca cttgcttttc atcagctaaa aacatgactg tttttccctg
     2641 ctgttctaat tctaacgcgt gttcttggta ttcatcaaat gataggttca attcaattaa
     2701 tcgtttacgt gtacccgcaa aatagcgtac tccattaatc gtaccgctga tcccagcacc
     2761 tgggtgggct gtaaaatcaa tcatatcgta tgttgctgct tcttgcagtt ttccgtattc
     2821 cacgattgct ttacctagtg gatgttcaga agcttgttct aaagtataaa aaatagatag
     2881 gacttctttt gaaccgacta gatctgtaac tttgggcttt ccttctgtaa tcgttccagt
     2941 tttatccaaa acaatgctgt ctaagtgagc agctgcttct aatgcttctc cgcctttaat
     3001 taaaattcca tttcgtgcac caacacccgt tcctaccata atagctgttg gggtcgctaa
     3061 acctaatgca catggacacg caatgactaa tacagagaca ctatggatca atgcgagttg
     3121 ccaatcacca gtgatcaaac ctgtagctat gagcgtgata aacgcgatga ctaatacgat
     3181 cggaacaaaa atccctgaga tccgatctgc gattttttgg attggggctt tgctcccttg
     3241 agcatcttcc accatttgaa tgatttgcgc taatactgtc tgcttgccga cttgtgtgac
     3301 ttccatgtgt agcagaccat tcgtattgat tgtcccgcca aaaagcgtgt catccggatt
     3361 cttttctaca ggaagatttt caccagtaag catactttca tctatcgcac tgcttcctga
     3421 aataattcga ccatctgctg gaatctgctc accaggacgg atgactagtt gatcaccaac
     3481 gactacttct tcaatcgcta acgtttcttc tttcccgttt cttatcactt gggcggtttt
     3541 tgtttgaaga gacatcaatt gtttgattgc attacctgtt ttcgtttttg ctgtatgttc
     3601 caaatatttg ccgagcaaaa tcagcgtgat gatcatactg ctgctttcaa aataaagatc
     3661 ttgggggtgt ccacggaaaa atccgttgta aatgctcaat gcaaaggcag ccgatgttcc
     3721 aattgctacc aatacatcca tatttggtgc tttcgtcttg attgcatggt acgcaccttt
     3781 ataaaaacga gcaccgacat aaaattggac aggagcagac agcactaact ggacgattgg
     3841 aaaatgaaaa aagtgaacaa tcgccgcatg gcttcctaac atcatagcaa tcattgacaa
     3901 catcaacggc aaagtcaaaa ccgtactcaa tataagatcg aatagcattt ttttcaaata
     3961 cgcttgcttt tcttctgcaa ttttttgttt gtgggcttca tcgtacaaaa ttgcaccata
     4021 accgatgttt tctacgcttt ggattaattt ttcagtggtc gtcccttcat atttgacact
     4081 tgctttttca gtggccaagt tgactgtggc attgaccaca ccaggttgtc cgttcagctc
     4141 tttttcgatc ctagcagaac aatttgcaca agtcatacct gtaatgacga acgtatccat
     4201 tttggtttct gctgttgcca tcaaatcacc tctgctttat aacccaatcc attgattgct
     4261 tgacaaatct tttcggattg aacttttgtt tcatcaaact tgaccgtacc gtttgctttt
     4321 ttcaaattta ctttcacttt ttgcacgccg tcaagtgcgc ttactgtttc ttctactctt
     4381 gctgcacaat gattgcagct cataccttcg attgaaaatt gttgtttcat tttttcttcc
     4441 tcctattgat gttgtttaca ttcacattga cctgggatac agttacattc gatcgtatct
     4501 actggttgct tttgttccag ttgtttcaaa atctcattga tatcttcttg agtcaaagtg
     4561 gcttcttcta ctagatccgc aatcgtctct ccaatccttt ttgcacagat atgggaaaaa
     4621 agattttccg tcgcactgcg aactgtttcc atctccgaga cagctggatg ataaatgaat
     4681 ttttttcctt cttgttctgt ccagatcact tcttttttta ccaaacgtcc cagcaaagtt
     4741 ttgatcgttg ctggtttcca atccatcgaa tcagctaaga tctgcgtgat ttcttgggct
     4801 gtcgtctgtc caagtgtcca aataacacgc atgatctccc actctgaatc actgatcttt
     4861 actggcgttt tgactttttc catttatttc acacctttcg tttacaaatg taatcattag
     4921 cttatgaata cagattacgc ttgtaaacaa aaactgtcaa ttatttttgc tctctatttt
     4981 caatttagtt aaaaattctt gtttctctcg tcttgtttta aggatgatca gtttatttct
     5041 tgcattagct acttggatca gctgctgtat tttcggttca ttgttttctt caaaatgcca
     5101 gataaaagat aaaaattcta agtattccct gtttagtttc tctgaaaatg attcaggcat
     5161 atctggccga gattttttgt ttatctttct ttttagagaa cgccaaacta ctcggtagat
     5221 ggcgagataa cgcggcactt taagccaaat gatcttatct gcttctttaa tcctttcctc
     5281 taatgtagaa gtataatttc cttcaacaat ccatgacgga ttagatacca taaacgattg
     5341 ctgttcttgc aaaaaccact tttttgcctg aatactgtag tctgttgtat gccataaaag
     5401 gtctagcggc agtagcggaa agtcggtgat ttctttgatc ttccttgcga gggtcgattt
     5461 tcccgaccca ctttgtccaa tcacgatgat cttcattctt ctccctcact tctctttgga
     5521 atttggttcg atcataccac ttataacgcc gcaaaaaaac aagggactgt gacaaaagtc
     5581 gtgtcaaagt cccttgtttc gaataaatgg tgttcaatca gctgaccttc agcattaact
     5641 caaaaaacgg caaaatatga ggagctattt tttcgtttgt tcctctaata ctcaggtcaa
     5701 atcgctgatt ttacaacctc ttccttaaac ggtgttctcc tgccaacacc tcggtcacaa
     5761 ggcacaatag tcaacaaaca tgagaagttg ttttttgaaa ttgctccttg tgactcggag
     5821 ttaaacggca ggctcactac ctctttctta aacggtgttc aaaagcttgc atctgcggta
     5881 ataagcccag caaaacgaaa aatatgagg
//
LOCUS       DACELF010000115         5905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.120, whole genome shotgun sequence.
ACCESSION   DACELF010000115 DACELF010000000
VERSION     DACELF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.120"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..667)
                     /locus_tag="IW687_002469"
     CDS             complement(95..667)
                     /locus_tag="IW687_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0951704.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW687_002470"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW687_002470"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0951705.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IW687_002471"
     CDS             complement(1352..1867)
                     /locus_tag="IW687_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0951706.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IW687_002472"
     CDS             complement(1869..2153)
                     /locus_tag="IW687_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0951707.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IW687_002473"
     CDS             complement(2187..3515)
                     /locus_tag="IW687_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0951708.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IW687_002474"
     CDS             complement(3529..3984)
                     /locus_tag="IW687_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0951709.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5852)
                     /locus_tag="IW687_002475"
     CDS             complement(3987..5852)
                     /locus_tag="IW687_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0951710.1"
                     /translation="MAVSTKTIYQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVE
                     HLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRII
                     NKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSK
                     VISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRY
                     METYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLI
                     ERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVW
                     YAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVR
                     RFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIME
                     AYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELE
                     ANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRK
                     VKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2003 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatt
//
LOCUS       DACELF010000116         5863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.121, whole genome shotgun sequence.
ACCESSION   DACELF010000116 DACELF010000000
VERSION     DACELF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.121"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2680
                     /locus_tag="IW687_002476"
     CDS             <1..2680
                     /locus_tag="IW687_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0951711.1"
                     /translation="SRFSIDIHPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNL
                     LADTIFVAHNIYFDYNFLNHELMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSE
                     SLGFIHENPHQADSDAEVTGQLLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYH
                     VIEEMKEKPEPLDPSLEIVDGLALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLM
                     YRKEQNRLMNAVYDHFTKDESKDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVS
                     LVLQQQIIEKDIPLLNQLLDQPIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQM
                     AVLVWLTQTKTGDLDELHLTNLNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIR
                     QSNFLIVNHAFLIQETQRKEPLLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQI
                     QQLEEPNQLFDRIQSYLPKEHEALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPN
                     GQYPEEVMMTKEMLDQLPLESGTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMR
                     IDLASLYSLFDQLDKQYLLMDQWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPS
                     TVWYPRYDRILYIGGTLKIGPNRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEA
                     PAVHQVSNQAYIDYLETALTGLIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREI
                     LAQGFGGSREKLLKRFILSEKSVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPM
                     VKARNAYLESEGINPFYQESVPKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYG
                     ARIIHALPKKVPIKELAMEEIYQDIHEFLKKNEEGS"
     gene            2727..3254
                     /locus_tag="IW687_002477"
     CDS             2727..3254
                     /locus_tag="IW687_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0951712.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3275..4465
                     /locus_tag="IW687_002478"
     CDS             3275..4465
                     /locus_tag="IW687_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0951713.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4505..5803
                     /gene="asnS"
                     /locus_tag="IW687_002479"
     CDS             4505..5803
                     /gene="asnS"
                     /locus_tag="IW687_002479"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0951714.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         1946 a         1101 c         1282 g         1534 t
ORIGIN      
        1 ttcacgtttt tcgatcgata ttcatccagg gcggaagatt tcaaaacaaa tccagcattt
       61 aacagggatc acgaatcaac gcgtccaaaa agcgccttat ttcgaagatg tcgcccaaac
      121 gatttacaat cttttggcag acaccatatt tgtcgcgcat aatatttatt ttgattataa
      181 ttttttgaac catgaactca tgcgatgcgg acttcctagt ttgaagatac caggcatcga
      241 tacagtagaa cttgcacaaa ttttcttgcc gacggaacca agctttcgct tagctgattt
      301 gtcagaaagc cttggattta ttcatgagaa tcctcatcag gcagatagtg atgccgaagt
      361 gacgggtcaa cttcttttgc tgatcgaaga acggatgaga aaattgccaa tcatcacgtt
      421 agagcagatt gcgcgattaa gtagacacac aggtatggat acgagtcgtt tcatttatca
      481 tgtgattgaa gaaatgaaag aaaaaccaga gccacttgat ccctcattag aaatcgtcga
      541 tgggctagct ttgcagaaaa aagaagtgga actattcaca tccgtccatt atggcgagcg
      601 gacatatcca agaaaaaaac aagcaaaaga aaaaatgttt ggtaaaacac tgatgtatcg
      661 aaaagaacag aacagactga tgaatgcggt ctacgaccat ttcacaaaag atgaatcaaa
      721 agatttgatg atcgaagcag ctacaggaat gggtaaaaca atcggttatc tgctgccatt
      781 gagttatttg gctacacctg aaaaacccgc tgtcatcagt accgtttcgc tagttttgca
      841 gcagcagatc atagaaaaag acattccatt gctgaatcaa ttgctagatc agcccataca
      901 acctgtaatt atcaaaagct atcgccacta tattgattta cagcgtttta aaggcacact
      961 tgtggaaccg ccagaacaaa aacaatatgc tttgtatcaa atggcggtct tagtttggct
     1021 gacgcaaacg aagaccggtg atttggatga attacattta acaaatttga accattcatt
     1081 ctttgcagat atcgcgcaca gaggtattgg atttttagca agaaatcagt cattttacga
     1141 acaagacttt gttcgtttct tgcaaaagaa aatcaggcaa agtaattttt tgattgtcaa
     1201 tcatgcgttt ttaatacaag aaacacagcg aaaagaacca ttgcttccag ctagtccatt
     1261 tttgttgatc gatgaagtac atcatttgcc ggaaatcgct gaaaaagtaa atgacaaacg
     1321 attaagtatt aactatttcc ataaacaaat ccagcaatta gaagaaccaa atcagctgtt
     1381 tgaccgaata caaagctatt taccgaaaga acatgaagcc ttacgctggt tatcgattta
     1441 tcgagaagaa ctttctgcgc tatgcgaagc acagatttgt atggcagaag gattaatgag
     1501 tttagtgagc ggcaagcaac ctaacggaca gtatccagaa gaagtcatga tgacaaaaga
     1561 gatgttggat cagctgccac tggaatctgg gacagccatt gatcacctgc agctttttta
     1621 tcaggagatc cagcagctac agtcacaaat gcgtctgact tttgagatta tccaagaaag
     1681 ctggacaaac cagatgcgga tcgatttagc aagtttatat agcttatttg atcaattaga
     1741 caagcagtac ttgctgatgg atcaatggct ggaagactgg cgagatcatt tgatccattg
     1801 gttcgttcct gggaacacag cgcagcagat gatttttcat ctccatgatt ttcaagcggc
     1861 tatgttgccg tcgactgttt ggtatcctcg atatgatcgt attctttata ttggaggaac
     1921 gctgaagatc ggccctaatc gacactatct ctctaagcgg cttggcattg aagcagcacc
     1981 tcttaaagtg attgcctctc cttatgatta tgagaaacag gcaaggatct atgtgccaaa
     2041 agaggcacct gccgtccatc aagtcagcaa tcaagcatat atcgattatt tagagacagc
     2101 acttacaggt ctgatcgaac aagaagatcg gccaatactt gtgttattca cttctcatga
     2161 tgttttgcag aaggtctatc agaaaatgca tctgtctatg ctagaaaaag gacgagaaat
     2221 actagctcaa gggtttggcg gaagccggga gaagcttttg aaacgcttca ttttgtcaga
     2281 aaaaagcgta ttgtttggcg ctgacagctt ttgggaaggt gtagatttgc ctggggatgc
     2341 tttgcagatc gttgtagtca cacgtttacc cttcgataat ccgcttcgcc cgatggtcaa
     2401 agctaggaac gcttatttag aatcagaggg gatcaatcct ttttaccagg aatccgtacc
     2461 taaagcagct ttgaagttac gccaagcatt aggtcgcttg ataaggacgg agacagatcg
     2521 tggcgtattg cttattttgg atcgaagact agtcactgca aaatatggtg cacggatcat
     2581 ccatgcatta ccgaaaaaag taccgatcaa agagctagct atggaggaaa tctatcagga
     2641 catccatgaa ttcttaaaga aaaatgaaga aggaagttag aggagctgtc ttttttcata
     2701 attaaaatct gctataatga aaaggaatga agataaagga gcgacgaccc ttgaagaaaa
     2761 aacagacaaa ggaaaaaacg attaatcgcg tgttacttgg cttgatcgta tttttattag
     2821 cggtcatcgt gatcagctcg attttttact tgcgttccag tcgtccggcg gtgcaggcga
     2881 agaaagaagc agtagaaatc gcaaagaaat atgcaaatct tgaggaagcc gatcattttt
     2941 attggtttac tcgaaagaag acatactttt cactgacagg tacaaatgat aaaggaaaag
     3001 aaatcgccgt tatcattcct aaatctggtg aaaaagtaaa agtattggat caaacaaagg
     3061 gactgactga aaacgaagca aaacagacaa ttgctgcggc ccatcctgaa atccaaatcg
     3121 aaaaagctgc tttagggatg tatgatgatc agcctgtgtg ggaagtagta ggaaaagaaa
     3181 caaatgggaa actgaattat tacctgattg gctttgaatc tggagatgaa gtcaagacaa
     3241 tcaagggaat ttaaaaggta aaggaatgaa tgggatggag ttatcaaaaa gaacaaaacg
     3301 attagaaccg tctgtcactt tggcagctgc ggcaaaggcc aaggcattaa aagcaaaagg
     3361 cagagatgtg ctgagtttga ctgtgggaga accggatttt gcgacaccag aaaacatcca
     3421 agaagcagca atcgaagcaa tccgcaacgg gaaagctagc tattatacac caacagcagg
     3481 aatcccagag cttcgacagg caatcgttga ttatctgaag aaaaactatg gacttgtcta
     3541 tgaaccttca caaacagtcg taacagacgg agcgaaattt gctttgtata cccttttcca
     3601 aacgatcctt gacccgcaag atgaagtcat cattcctgtg ccttactggg tcagttacgg
     3661 tgagcaagta aaactggcag aaggacttcc tgtctttgtg aaaggggaag aatccaatag
     3721 ctggaaagtt actgttgaac agctagaaca agcacgttca gaaaaaacaa aagccgtaat
     3781 catcaattct ccatcgaatc cgactggaat gatttatagc aagaatgaac tgcaggcaat
     3841 cggtgaatgg gcagtaaaaa atgatatttt gatagttgct gatgatattt atggtcgttt
     3901 agtttataat ggcaatgagt ttactccaat tgcaacgatt tcagaagcaa tcaaaaatca
     3961 gactatcatc atcaacggcg tgtctaaaac atatgcgatg acaggctggc gcattggtta
     4021 tgcggtggga aatcctgtga tcatcaatgg aatgatcgct gttgcttccc agtcaaccag
     4081 taatccgaca gcagtcagtc aatatgcagc tgttgaagca ttgaaaggcg agcaagatac
     4141 agtggaagaa atgcggaacg cctttgaaga acgcctgaat acgttatatt cgcttgtggc
     4201 agaactgccg ggtgtttctc ttgaaaagcc acaaggagct ttttatcttt ttcctaatgt
     4261 aaaagaaacg ctacgcatgt gtaaatatga aaatgtcaca caatgggtag aagatctttt
     4321 agaagaaaca ggggtagcgc ttgtgactgg cgaaggcttt ggggcaccag aaaatgtccg
     4381 catgagttat gctactgatc gcgctacgtt agaagaagcg gtacgcagaa tcaagcaatt
     4441 tattgagagt aaaagtcaaa attaatccta aagaaattca aggaatataa aaggagagtc
     4501 atacgtggaa caaattcaaa tcatcgattc gaaaaaccat gtgggagaaa cagtaacaat
     4561 cggcgcttgg gtcgcaaata agcgatcaag cggaaaaatc gcatttttac aattaagaga
     4621 cggaacagcc tttttccaag gtgtagttgt aaaaagcgaa gtgtcggaag aagttttcca
     4681 aacagctaaa aatctacacc aagaaacatc cgtgttagta actggagaaa tccgtgaaga
     4741 cagtcgttca aaatttggtt atgaaatcgg aattacaaat gtggaagtgg taagcgagag
     4801 ccacgattat ccgatcacac caaaagaaca cggaacagac ttcttgatgg atcatcgtca
     4861 tttatggctg cgttcttcaa gacaacatgc gattatgcag atcagaaatg aaatcattcg
     4921 tgcaacgtat gaattcttta atcaacatca ttttgtcaaa attgatccgc cgatcttgac
     4981 tggttctgca ccagaaggca ctacggatct attcgaaaca aattactttg atcaaaaagc
     5041 ttatttatct caaagtggcc aattatacat ggaagctgct gctatggctt tcggaaaagt
     5101 cttttcattc ggtccgactt tccgtgctga aaaatcaaaa acacgacgtc atttgatcga
     5161 attctggatg atcgaaccag aaatggcttt cactcatcaa gaagaaagtt tagaagtaca
     5221 ggaacaatat gtagcattct tagtacaaaa tgttttagat cattgcgaat atgcattaga
     5281 tgtactagga cgagacaaag aagtcttaaa aacttataca aaattgccat tccctcgtat
     5341 tacttatgat gatgcgatcg aattactgaa gaaaaatgga tttgatgaca tcgaatgggg
     5401 cgaagacttt ggttcacctc atgaaacgtt cattgcgaat tcattcgaac agcctgtctt
     5461 tatcctaaac tatccaaaag ccatcaagcc attctacatg aaaccacatc caacaagaga
     5521 agacgtggtg atctgtgcag atatgatcgc accagaaggt tacggtgaaa ttattggcgg
     5581 aagcgaacgg gcaacagact atgatttctt gctagaacaa atccgtaatc acggtttgga
     5641 tgaaaaagaa tattcatggt atcttgatct acgtaaatac ggttctgttc cgcactctgg
     5701 ttttggtcta ggtcttgaac gaacagtcac ttggattgct ggtatcgacc atgtacgtga
     5761 agctattcca ttcccacgtt tgttgaaccg aatttatcca taagaggtga tgaaaaagct
     5821 gttctgcatc aagtaattag aacagccaag caaaaacagc tgt
//
LOCUS       DACELF010000117         5748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.122, whole genome shotgun sequence.
ACCESSION   DACELF010000117 DACELF010000000
VERSION     DACELF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.122"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..2193)
                     /locus_tag="IW687_002480"
                     /pseudo
     CDS             complement(43..2193)
                     /locus_tag="IW687_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2339..2914
                     /locus_tag="IW687_002481"
     CDS             2339..2914
                     /locus_tag="IW687_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951715.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3128..3823
                     /gene="vanR"
                     /locus_tag="IW687_002482"
     CDS             3128..3823
                     /gene="vanR"
                     /locus_tag="IW687_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0951716.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3801..4955
                     /locus_tag="IW687_002483"
     CDS             3801..4955
                     /locus_tag="IW687_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0951717.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(5053..>5748)
                     /locus_tag="IW687_002484"
     CDS             complement(5053..>5748)
                     /locus_tag="IW687_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0951718.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT         1777 a         1068 c         1122 g         1781 t
ORIGIN      
        1 tagtttgaaa tgcaattttc ttctttcagc tttttatttc ccacttgtaa atcggtagat
       61 ggtggctcct tttccagttc cataatgtgg atttgcatct gcatgtagtg atgaaacacc
      121 tagctgcatt ctcataccat ctgacgaaga tgttgtaccg tcgccccaat agaaaggcaa
      181 ttgtaattta tgatgaaagt ttactaatat ggcttgggct ttattcatgg catcttcata
      241 catgcgccat tgagatacat tggctagttg cttatatgta agtccgggtg tggcttcggc
      301 catcttgctc aagccaatat tcattcccat tcctaaaagg gcagccatga taatgattgt
      361 ttcttcctta tctggttttc gattattgga agcatgagtg aattgctcat gaaatcctgt
      421 tatatgggcc acatccatga gtaaatcagt taattttatt cttggtagca tctgataaag
      481 gcttgcacta aatttttttg cttcttctgg aacatctttt tctaagcgtg caagtgatag
      541 ctttcctttt tcaagagaaa ccccatctaa cttattggaa ttggcagcta accactttaa
      601 cctttcatta aagctgctgg ttctctccgt tatataatct tcgaatgata aactaactga
      661 taatctcgta ttccccttcg attgattcca tgtatcttcc gaaaacaaat attcctcaaa
      721 atccctatat tgtctgctgc caacaatgga aacatctcct gcccgaacat gctcccgaag
      781 ttctgttaaa acagccattt catagtaatg acgattaatt gttgtaccat catcctcgta
      841 taaatgtctt ttccatcgtt ttgaaataaa atccacaggt gagtcatcag gcacttttcg
      901 ctttccagat tcgttcattc ctcggataat ctcaacagct tgtaaaagtg gctcatttgc
      961 ctttgtagaa tgaaattcca atactcttaa tagcgttggc gtatattttc ttagtgaata
     1021 aaaccgtttt tgcagtaagt ctaaataatc atagtcggca ggacgtgcaa gttcctgagc
     1081 ctcttctact gaagagacaa aggtattcca ttcaataacc gattctaaaa ccttaaaaac
     1141 gtctaatttt tcctctcttg ctttaattaa tgcttgtccg atgttcgtaa agtgtataac
     1201 tttctcattt agctttttac cgttttgttt ctggatttcc tcttgagcct tacgaccttt
     1261 tgataacaaa ctaagtattt gcctatcatg aatttcaaac gctttatccg ttagctcctg
     1321 agtaagttgt aataaataga tggttaatat cgaataacgt ttattttctt gaaagtcacg
     1381 gaatgcatac ggctcgtatc ttgagcctaa gcgagacagc tgcaacaggc ggttacggtg
     1441 caaatgacta atttgcactg tttctaaatc cattcctcgt atgtattcga gtcgttctat
     1501 tatttttaga aaagtttcgg gtgaaggatg acccggtggc tcttttaacc aacccaatat
     1561 cgttttattg gattcggatg gatgctgcga ggtaataatc ccttcaagct tttctttttg
     1621 ctcatttgtt agagatttac taaccgtatt aaatagcttc ttttcagcca ttgcccttgc
     1681 ttcccacacc attctttcaa gtgtagtgat agcaggcagt ataattttgt tttttcttag
     1741 aaaatctatg cattcatgca gtagatgaat ggcatcacca ttttccaaag ctaattgatg
     1801 aaggtactta aatgtcattc gatattcact cagggtaaaa gttacaaagt cgtattcact
     1861 tcgaatttct ttcaaatgat cccaaagtgt attttccctt tgaggataat gatcaagcga
     1921 ggatggacta acaccaatct gtttcgatat atattgtatg accgaatctg ggatgctttt
     1981 gatatgagtg tatggccaac cgggataccg aagaacagct aattgaacag caaatcctaa
     2041 acggttttct tccctccttc gcttattaac tatttctaaa tcccgtttgg aaaaagtgaa
     2101 gtaggtcccc agtatccatt catcttcagg gatttgcata aaagcctgtc tctgttccgg
     2161 tgtaagcaat tctctacctc tcgcaatttt cattcagtat cattccattt ctgtattttc
     2221 aatttattag ttcaattata tatcaataga gtgtactcta ttgatacaaa tgtagtagac
     2281 tgataaaatc atagttaaga gcgtctcata agacttgtct caaaaatgag gtgatatttt
     2341 gcggaaaatc ggttatattc gtgtcagttc gactaaccag aatccttcaa gacaatttca
     2401 gcagttgaac gagatcggaa tggatattat atatgaagag aaagtttcag gagcaacaaa
     2461 ggatcgcgag caacttcaaa aagtgttaga cgatttacag gaagatgaca tcatttatgt
     2521 tacagactta actcgaatca ctcgtagtac acaagatcta tttgaattaa tcgataacat
     2581 acgagataaa aaggcaagtt taaaatcact aaaagataca tggcttgatt tatcagaaga
     2641 taatccatac agccaattct taattactgt aatggctggt gttaaccaat tagagcgaga
     2701 tcttattcgg atgagacaac gtgaagggat tgaattggct aagaaagaag gaaagtttaa
     2761 aggtcgatta aagaagtatc ataaaaatca cgcaggaatg aattatgcgg taaagctata
     2821 taaagaagga aatatgactg taaatcaaat ttgtgaaatt actaatgtat ctagggcttc
     2881 attatacagg aaattatcag aagtgaataa ttagccattc tgtattccgc taatgggcaa
     2941 tatttttaaa gaagaaaagg aaactataaa atattaacag cctcctagcg atgccgaaaa
     3001 gccctttgat aaaaaaagaa tcatcatctt aagaaattct tagtcattta ttatgtaaat
     3061 gcttataaat tcggccctat aatctgataa attattaagg gcaaacttat gtgaaagggt
     3121 gataactatg agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt
     3181 tgaattatac ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc
     3241 attggaatgt atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg
     3301 cacaagcggc cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat
     3361 gctgaccggg aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga
     3421 ttatataacg aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg
     3481 ccgatacaaa aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg
     3541 ccttgtcatt aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac
     3601 tcccaccgag ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc
     3661 cgagctgcta tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat
     3721 caccgtgcat atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata
     3781 tataaaaacg gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca
     3841 aactagaacg aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg
     3901 tgttgtatat tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg
     3961 aaaacaaata tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga
     4021 acaatataga tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg
     4081 tcatgctttc aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta
     4141 ttcagaacga agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc
     4201 tcaacacatt aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa
     4261 gaaaaaatga cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta
     4321 tcggttattt gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt
     4381 atgtgcatat cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg
     4441 agattacacg gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact
     4501 atatgctggt gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg
     4561 cggttattca cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag
     4621 tctttaacaa cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca
     4681 ttaccgcggg cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc
     4741 caaaagataa gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt
     4801 ccgatacggg tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg
     4861 gagggcagat ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag
     4921 cgatgccaga cttggttgat aaaaggaggt cctaagagat gtatataatt ttggctcttt
     4981 gtcaaatgat gttggtaaag agaaatctta gaataattgg agatcaagtg gatcagttcc
     5041 tcttgatctt ttttagttga aaatcaatgc gttgccatga cgaatcatga tgcgcaattt
     5101 gaaattataa aagctacgaa agccatatgc atttcgtttt aacactttga tatggttgtt
     5161 cagacattca agtgggccat ttgaataact ttgttgtaaa gcatttttga tttgtggcag
     5221 aaatttctgg aacgttctca aggtcgtctg atagccttcg ggaagcatga tattattggt
     5281 aagtagttct tcaaacgata cgtagtctcg ttcctttaca gcgtaaagaa agtcttgata
     5341 taactgatag cctacacgca aagcagggct ccctttcagt aagcgatcca caatatcggt
     5401 ctctgttaaa tgagctttga agccaggtcg ccaaaggcgt cgagtataat caagcttgcg
     5461 ttcatctttt tgaagaagtt tccagtatct ttttaattgc ttacctaatt gatagtgttt
     5521 ttgttctttg ctttttaata gttggttcgt ttctttgatt cgatgattgc gaaaggtacg
     5581 accaatatgt tggacgatgt gaaaccggtc aataatcagc tgtgcttttg gaaacagctt
     5641 tttaacgagc gatacataag gagcgtacat atcaatcacg atccattcga ccgcttcacg
     5701 aatttctcgt gaaaaatgac taaaataacg ttttaaaaaa ggcagttg
//
LOCUS       DACELF010000118         5689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.123, whole genome shotgun sequence.
ACCESSION   DACELF010000118 DACELF010000000
VERSION     DACELF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.123"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            213..1406
                     /locus_tag="IW687_002485"
     CDS             213..1406
                     /locus_tag="IW687_002485"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0951719.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1536..3008
                     /locus_tag="IW687_002486"
     CDS             1536..3008
                     /locus_tag="IW687_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0951720.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3091..3879
                     /locus_tag="IW687_002487"
     CDS             3091..3879
                     /locus_tag="IW687_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0951721.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3946..4614)
                     /locus_tag="IW687_002488"
     CDS             complement(3946..4614)
                     /locus_tag="IW687_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0951722.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4729..>5689
                     /locus_tag="IW687_002489"
     CDS             4729..>5689
                     /locus_tag="IW687_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951723.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDV"
BASE COUNT         1700 a          941 c         1248 g         1800 t
ORIGIN      
        1 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa
       61 tgtaaaatat ttttatattt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      121 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      181 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      241 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      301 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      361 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      421 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      481 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      541 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      601 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      661 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      721 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      781 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      841 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      901 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      961 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1021 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1081 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1141 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1201 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1261 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1321 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1381 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1441 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1501 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1561 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1621 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1681 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1741 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1801 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1861 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1921 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1981 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2041 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2101 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2161 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2221 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2281 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2341 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2401 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2461 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2521 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2581 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2641 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2701 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2761 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2821 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2881 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2941 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3001 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3061 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3121 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3181 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3241 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3301 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3361 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3421 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3481 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3541 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3601 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3661 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3721 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3781 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3841 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3901 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3961 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4021 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4081 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4141 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4201 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4261 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4321 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4381 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4441 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4501 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4561 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4621 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4681 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4741 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4801 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4861 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4921 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4981 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5041 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5101 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5161 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5221 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5281 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5341 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5401 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5461 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5521 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5581 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5641 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttc
//
LOCUS       DACELF010000119         5662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.124, whole genome shotgun sequence.
ACCESSION   DACELF010000119 DACELF010000000
VERSION     DACELF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.124"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..542)
                     /locus_tag="IW687_002490"
     CDS             complement(72..542)
                     /locus_tag="IW687_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0951724.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IW687_002491"
     CDS             872..2512
                     /locus_tag="IW687_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0951725.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IW687_002492"
     CDS             2666..3988
                     /locus_tag="IW687_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0951726.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4487)
                     /locus_tag="IW687_002493"
     CDS             complement(4056..4487)
                     /locus_tag="IW687_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951727.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4552..5388)
                     /locus_tag="IW687_002494"
     CDS             complement(4552..5388)
                     /locus_tag="IW687_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0951728.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1127 c         1037 g         1802 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4201 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4261 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4321 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4381 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4441 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4501 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4561 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4621 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4681 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4741 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4801 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4861 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     4921 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     4981 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5041 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5101 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5161 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5221 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5281 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5341 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5401 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5461 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5521 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5581 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5641 aagaggtcgt gagcctgccg tt
//
LOCUS       DACELF010000120         5612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.125, whole genome shotgun sequence.
ACCESSION   DACELF010000120 DACELF010000000
VERSION     DACELF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.125"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..310)
                     /locus_tag="IW687_002495"
     CDS             complement(<1..310)
                     /locus_tag="IW687_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951729.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     S"
     gene            complement(449..694)
                     /locus_tag="IW687_002496"
     CDS             complement(449..694)
                     /locus_tag="IW687_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0951730.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            887..1687
                     /locus_tag="IW687_002497"
     CDS             887..1687
                     /locus_tag="IW687_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0951731.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1706..3574
                     /locus_tag="IW687_002498"
     CDS             1706..3574
                     /locus_tag="IW687_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0951732.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3567..4058
                     /locus_tag="IW687_002499"
     CDS             3567..4058
                     /locus_tag="IW687_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0951733.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GIRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4046..4600
                     /gene="srlA"
                     /locus_tag="IW687_002500"
     CDS             4046..4600
                     /gene="srlA"
                     /locus_tag="IW687_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0951734.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4618..>5612
                     /locus_tag="IW687_002501"
     CDS             4618..>5612
                     /locus_tag="IW687_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0951735.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1965 a          891 c         1145 g         1611 t
ORIGIN      
        1 cggaacggtg ggcaataaga aatagaagtg ctttgtcaga gttttctaat ggaagagaag
       61 gtacagtgct ttcttgcatt tcagatgttg aagaaatgct aaacgactga atacttgatt
      121 ggctatgttg cgaatctaca ttaccagtag aagtattagt aatggaagta gtagaagtac
      181 ttttagttga accaacctca gttgttgaaa tggctgctga agattcttta acagaattct
      241 ttgttttata tccacaggca gaaaaaagta ttacagttcc caacaagcct gcgtaaatcc
      301 ctttatgcat gatagctcct cctaaaatag attatcttta ttcagtatat cacttaaaca
      361 aagaaaagca gatggtttaa aatgaaaaaa ccttcccaat aaagggaagg ttcatgaaga
      421 taataacttt tttaataatt atagctttct aatatctttc atcaactttt ctagtaatca
      481 ttgggaaaaa tttcaagcag tagatcgcac aaatacctac gacgatataa atgatcttcg
      541 caaaaatagt tgttgaacca ccagcaatca tagctactaa atcaagttcg aacaagccta
      601 ccaacagcca gttcaaccct cccacaatca ataaagttaa tgcaatagca tctaagtttt
      661 cattagaatt ctcctttctt aaatgataaa acaattatat atcaaaaaga gaaacattgc
      721 ttatataacg ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt tttttgctct
      781 tccattagta cacatttttt agctgaagct gtaaatttca tgtcttaatt ttttttagtg
      841 agcttttata ctggatttgt aagaaaaaag aaggagagga tcacatatgt cggattggtt
      901 ggatattgca ggaaaagtag taattgtaac tggaggttcc tcaggaattg gaagaagtat
      961 cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa ctgaatgccg
     1021 tatacagcat gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa cggacataga
     1081 agaagggata tataaagtaa tgaaacattt caagacgatc gatggtctag tcaataatgc
     1141 aggaataaat attccttctc ttctaataga tagaaatcat ccaaaaagca agtatgaatt
     1201 atcagaacaa gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat atttgatgag
     1261 ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga atctttcttc
     1321 tgaatcagga ttggaaggat cagaaggaca gagctgttat gctgctacaa aagcagcgat
     1381 gaacagtttt actcgttctt gggcaaaaga gttaggaaag aggaatgttc gtgtggtagg
     1441 tgttgcacca gggatactgg aagaaaccgg tttgagaaca caagaatatg aagaagctct
     1501 ttcttatacg agaggtataa gcgtcgaaca gctgagaaac ggttacagtc gaacaagtac
     1561 gatccctcta ggaaggagtg gtaagctgca agaagtggcg gatctagtct gctattatct
     1621 ttcagaaaga tcaagttata tcactggagt cacgactaat atttcaggag gtaaaacaag
     1681 aggataaagg atagggggga cgcacgtgac gctagtaaac agatggtacc aattattgaa
     1741 attactagtc actcacaaga aagtttcaat ccaggaagtg caaaaagaaa tcaaaaccag
     1801 taatcaaacc ataaaaaaag atattgggga gttgaatgaa gaaattgccg gtattgcaaa
     1861 aatcatccaa aaaaacaaac attttgaact tgaaatttat gattttgatt tttttgatga
     1921 gatcatgcaa ggaaaattga aaacagccag tgatttcaat tcggcatcca aaagaatcgc
     1981 ttatttacta aataggctga tcgaaacaga ggatttctta cgtatggatg atttgtctga
     2041 agaagtcggt gtcagcagag gaacagtggt caaggatcta aagacattaa aagagatgat
     2101 caatgatttt gatgtaaaga ttacaggcac accaaataaa ggactaagaa taataggaga
     2161 tgagctagaa ttacgactac ttttatttta ttttgtttct ccctactttt cagagacgaa
     2221 aacagaacag tttctagaag aaaaacaagc aatcaaacat tttcagaaga agacgaatgc
     2281 tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt atttctttga atagaatctc
     2341 tagtaactat ctgttgaaga agcccatcaa tcgttacagt gggtgttttg aatatatcca
     2401 agaattcaat gaactgatca atcagttgga agaaatctat gaactaactt tgagccaata
     2461 tgaaaaagat tttatcagtt ttcctttgaa catcatcaac acaagtataa cgatgataag
     2521 gaaagctaac tctgatttgc gcttatattt cgaccccatg atgatgcgta ttcgacgatt
     2581 agatctcatt gatctggatg agtccttctt atatcaagag atgaaacagc acctctcttt
     2641 attgataaat cgggtaattt ttcgttatcg attgactgat ttattttatg gtgagataga
     2701 aaaaaagtat cctttttctt accaaatcgc tagcgaatgc atacaagcat tagaagctat
     2761 cttgcaacat aagatctcta aagtagagat cagctaccta acgttgtatt tggagttagc
     2821 atttcgatat caaaaagagc atttttcctc taaaaaagta gccattgttc atacgacagg
     2881 aaaaggaaca gcattaatga tccaaagaca aatcaaaagg atattgggga gggatataca
     2941 aataacacat gtttcagaag aacaatatga aaaagtagat ctaaacaaat atttcgctgt
     3001 ttttacaacg attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc atttgactaa
     3061 tctttttgat gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa agcgattagc
     3121 cgggtttcat tctactgttt ttttctatca aatatggaaa gaaaaaaaga cgtatcaaga
     3181 atatttaaac aaattagtcg ctcctttgat cagaagctat tatgtggatg aagaatttga
     3241 aaaaaggatc ttgaaaagag aatatcagca aagcacattt gttaacaaag aatttgcttt
     3301 tcctcacaca accaaccagc tatctcaaca cattgtttta tcagtagggc taataactga
     3361 tcaaggttta gcagaaacag gaggaaatgg agttattttt ttatttgcca tacctgaaga
     3421 aatgacggct gaagttgaga atgaattatt tgagctatac gatatcctat ttgaactcat
     3481 aaaaatggga atagatgagt ttattgaatt agcaccgaca caagaatcat ttttaaaact
     3541 aatcaaaagt aaaggagaga gacttcatga atgatatgta tctattcata gttttcgcag
     3601 cttcagctta tgtgctgcaa gcagttatca gttggaagca actaaagcat ttcaatcaaa
     3661 catatagcga actgcgaaga aaaggaaaag tagcgattgg tatacgacct gggaaaatcc
     3721 gttcaggcac gattgttatg ctagctatag acgatcatga tcaagtttta gaagtcagaa
     3781 aaatgcaagg ggttacatct ttggcaaaat ttcattcatt acctgtctat gcgggtgaag
     3841 atatccatta catcgatcgt taccatccct cagttcgagc ggaagatcgt ttgacacaag
     3901 cagctttaga aaatgccaga gaagtatact tacgaataga ggcaggaaat gaaaaaagtg
     3961 aacaaccttc attgcctttt attgctatgc tcaataattt tcggatatgg aaaagtacga
     4021 tcaaagaaaa aaggagtgac aaacgatgga tatcataaca aaatttgctg agggatttat
     4081 gaggctgttc caaacaggtg cagaaacatt cataagctgg atgacaggaa tcgtaccagt
     4141 tgtcttgatg ctgatggttg caatgaatac gctgattgct tttttaggtg aagaccgagt
     4201 aacgaaagta gccaaattat cagcgaaaaa tccgcttact cgttaccttg tgttaccgtt
     4261 cttatctgct tttatgttgg gaaatcctat gtcatttaca atggctcgtt tccttccaga
     4321 atactacaaa ccaagctatt atgctgctca agctcaattt tgtcatacga gcaatggtgt
     4381 attcccccat attaatcctg gagaattgtt tgtatggatg gggattgctc aagggatcga
     4441 aaccttaggg ctaaattcga tggagctggc tatccgatac ttgcttgtgg gactgctgat
     4501 gaactttatt ggtggttgga taactgattt tactactgga tttgtatgca gacagcaagg
     4561 aatcgttctt agcaaaaaag tagagctttc agttgattag agaaaggaga gttttaaatg
     4621 tatggtgata ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt gattattagg
     4681 ccgacaaaag ataaacacaa gttcatctat gtcacaggtg gaggtgagag gccaatgatc
     4741 gtagataaaa tagaagaact gacaggtatg gagtctatca atgggttcaa aacatcaata
     4801 cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag ggactcttag atgtggaatt
     4861 tatcctaaaa aagggatacc aaccatcaat atcgttccca caggaaaaag tggcccatta
     4921 gcacaatata tcactgaaga tatttatgtt tcagcagtag ggttagaaca aatacagcca
     4981 ctaatggaaa ctgatgataa caaagaaaca gtaaatactg catctccaga taaaaagtac
     5041 aaatatgata cttctaaaaa gataaccgag caaaaagcag agcagcaaaa ctggatagca
     5101 aaaattggaa tgggcgcggg aaaagtagtt gctacattca atcaatcagc aagagaagca
     5161 gtagatacga tgatccggac agtgatccct tttatggctt tcgtttccct tttgattggt
     5221 gtgatacaag gttctgggat cggtaatgta tttgctaaca ttatggcacc tttagcagga
     5281 aatgcagttg gattaacaat cattggcttt atttgttcac tgccattttt atctcctttg
     5341 cttggtccag gggcagttat tagccaagtg attggaacat tgatcggtgt agagattggg
     5401 aaaggaaata ttcctcccca attagcatta ccggcactat ttgctatcaa tactcaaaat
     5461 gcatgtgatt ttattccagt cggtctaggt ttggaagaag caaaagctga gacagtagaa
     5521 gtaggtgtac catcagttct ttattcaaga tttcttaacg gcatcccacg agttatcatt
     5581 gcttgggtag caagttttgg attatatagc tg
//
LOCUS       DACELF010000121         5466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.126, whole genome shotgun sequence.
ACCESSION   DACELF010000121 DACELF010000000
VERSION     DACELF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.126"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW687_002502"
     CDS             <1..936
                     /locus_tag="IW687_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0951736.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW687_002503"
     CDS             939..3122
                     /locus_tag="IW687_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0951737.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW687_002504"
     CDS             3119..4120
                     /locus_tag="IW687_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0951738.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW687_002505"
     CDS             4135..5049
                     /locus_tag="IW687_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0951739.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW687_002506"
     CDS             5294..5410
                     /locus_tag="IW687_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0951740.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1727 a         1049 c         1156 g         1534 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgc
//
LOCUS       DACELF010000122         5464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.127, whole genome shotgun sequence.
ACCESSION   DACELF010000122 DACELF010000000
VERSION     DACELF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.127"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1106
                     /locus_tag="IW687_002507"
     CDS             132..1106
                     /locus_tag="IW687_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0951741.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IW687_002508"
     CDS             1088..1549
                     /locus_tag="IW687_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0951742.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IW687_002509"
     CDS             1599..2063
                     /locus_tag="IW687_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0951743.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IW687_002510"
     CDS             2077..2826
                     /locus_tag="IW687_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0951744.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IW687_002511"
     CDS             2826..3656
                     /locus_tag="IW687_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0951745.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IW687_002512"
     CDS             3656..4303
                     /locus_tag="IW687_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0951746.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IW687_002513"
     CDS             complement(4401..5177)
                     /locus_tag="IW687_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0951747.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IW687_002514"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IW687_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1874 a          738 c          972 g         1880 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 ttta
//
LOCUS       DACELF010000123         5322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.128, whole genome shotgun sequence.
ACCESSION   DACELF010000123 DACELF010000000
VERSION     DACELF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.128"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..210
                     /locus_tag="IW687_002515"
     CDS             10..210
                     /locus_tag="IW687_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0951748.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IW687_002516"
     CDS             288..962
                     /locus_tag="IW687_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0951749.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="IW687_002517"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="IW687_002517"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0951750.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="IW687_002518"
     CDS             1630..2337
                     /locus_tag="IW687_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0951751.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="IW687_002519"
     CDS             2327..2617
                     /locus_tag="IW687_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951752.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="IW687_002520"
     CDS             2876..4060
                     /locus_tag="IW687_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0951753.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="IW687_002521"
     CDS             4075..4194
                     /locus_tag="IW687_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0951754.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            4940..>5322
                     /locus_tag="IW687_002522"
     CDS             4940..>5322
                     /locus_tag="IW687_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0951755.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT         1780 a          858 c         1229 g         1455 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaatttat gtgcgactcg ttccttagtg aaaagctaag gcactaataa
     4261 aagaacgaaa aagtttggtt attaggagaa ctgataatct gacaggtgga atgatgaagt
     4321 aacgctctga aacgcctgcc ctgagcctcc gacaagcatt gtatgaggaa actcagaatg
     4381 tttgaagctc ggtaaagacg gctgaaagat accgtaatag ttgatgaaca tatatcttct
     4441 atcgaaaact atccagaggt gggtagcgta tccaacaggt cgggggtcta taaaatatct
     4501 atggttagaa tgttctataa ggaactgacg aaacagcgaa tgtacgggtc taaatttgaa
     4561 ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg tggcaaagta agaatatagg
     4621 ttatgaaatg ccatataacg ttacaggcgg tatcaagcca gcaggcttat agctagtgac
     4681 ctaaggatat atatgtatag ataagattat cggaacgagg aaaggtattg ggttctcatc
     4741 aagagaataa actgacgaag aacaataagc agttgaacta atgctgaaaa gcagagggcg
     4801 aacctaagaa attcttgtaa tgagaatgga ggaatgccct caagtcgtgt atatattaga
     4861 aaagtattat gttgtttcaa taaggattac gagtaagata aaaaagctca ctcaatttca
     4921 agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa acattgtgtg tagaagattt
     4981 aagacacgct gaatattacg aaatgcaaaa cacctttgat gatttatatg ccaaaagtaa
     5041 gaatggagat attttcactc atcttatgga tattatccta tcgagagaaa atatattact
     5101 ggcatatcga aatatcaaag caaatgcagg cagtaaaaca gcaggaacag acggtacaat
     5161 aattaaagat attggcaaat tgccagcaga aacagttgtc aaaaaagtaa gatacattgt
     5221 tgcaggcagt ccacatgggt atcgacctaa gccagtgagg cgaaaagaaa taccaaaacc
     5281 aaatggtaaa accagacctc taggtattcc ttgtatgtgg ga
//
LOCUS       DACELF010000124         5249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.129, whole genome shotgun sequence.
ACCESSION   DACELF010000124 DACELF010000000
VERSION     DACELF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.129"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..399)
                     /locus_tag="IW687_002523"
     CDS             complement(<1..399)
                     /locus_tag="IW687_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0951756.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDIND"
     gene            complement(818..1036)
                     /locus_tag="IW687_002524"
                     /pseudo
     CDS             complement(818..1036)
                     /locus_tag="IW687_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1067..2281)
                     /locus_tag="IW687_002525"
     CDS             complement(1067..2281)
                     /locus_tag="IW687_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0951757.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(2622..2864)
                     /locus_tag="IW687_002526"
     CDS             complement(2622..2864)
                     /locus_tag="IW687_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0951758.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2896..3453)
                     /locus_tag="IW687_002527"
     CDS             complement(2896..3453)
                     /locus_tag="IW687_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0951759.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(3466..3654)
                     /locus_tag="IW687_002528"
     CDS             complement(3466..3654)
                     /locus_tag="IW687_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0951760.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(3667..4233)
                     /gene="amaP"
                     /locus_tag="IW687_002529"
     CDS             complement(3667..4233)
                     /gene="amaP"
                     /locus_tag="IW687_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0951761.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1579 a         1074 c          796 g         1800 t
ORIGIN      
        1 atcattgata tcaaattctg gaaagaatgt atctccatct tcaatattta aatctacttc
       61 tgtgatatac attttatcta ctatttgcaa agcttcctta aatagtccat atccaccaga
      121 tatgtataca tctcgtcctt tagccaataa taatgcatct tctaatgatt taactgaaac
      181 taaattatct ccttgatact ctgttgtggt ggaaacaaca atattcattc tattaggcaa
      241 cggatgaccg atttcttcat aagactttcg ccccataata accacattac ccgttgttaa
      301 ctctctaaat tgcttttgtt ctccctttat tttccatggt atattaccat tcttgcctat
      361 aacattattc tttgacctcg caacaatcaa acctatcatc tatttcatcc tccataatat
      421 gatgtcgtac ctgtgattcc atcaagtgca ccacatctta acaaactcaa aattatctcg
      481 aactaaccag ctcgacaaat tccttttttg tcttcatctg cattatatca tattgttata
      541 agcattgcca tttcttttct gcttttgaaa agttacttat cagcctatgt tttgtaaagg
      601 gtgatattca gcatttctca cgcaacaagc ccttgacaaa ccatagacta ataagtttta
      661 aggtaatcaa gcagaaatga aatagtctgc tgtttcacgg caacaaaaaa gagataccaa
      721 cacacttatg tagtatctct tagtctagta gcaatattct tttgtttgca agcaggtaaa
      781 catgggctgt ttcttaatat ctttttgttt gggaaacacc tttttcccat cctttcaccg
      841 ttgccatctc tgctgcgcac aatgccacag ggataaacca tagcaaaccg cctaataaca
      901 agaaaaatac taagctgaag cctgaggtcg cgaatgtggg atattcatat aatgaaacga
      961 ccattgagat ggtcatagcc aaaaagccaa agaacgttaa tttttttgca ggaattttcg
     1021 ttttgctttg ttgcatgaat cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt
     1081 tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc ttttgtagta gtgatcgttg
     1141 taaagtgcat cgccgatttt aaaatcactg ttgtcagcac ataggcatac acggcagtaa
     1201 tgatgacacc aatcacttga atccctaaga agtaccagct gcttggattc gttagtaaat
     1261 ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt gaatccgccc atgccatgaa
     1321 caccccacac atcaagtgca tcatcccatt gtttcttttt acgaaaatca acagcagcat
     1381 gacagacgat acccgcaatg atcccaatca gcatggcact ttttgcttct acataaccgg
     1441 cacaaggcgt aatggctgcc aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa
     1501 agccatttcc ttttactttt gcaatgatca gccaagtaat catcgcaaat gcaagagcga
     1561 tgaatgttga accaaatgca ttgactgctt gttcatctgc tcgaagtgca ccaccggaat
     1621 taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat agcagctgcc ataagactgc
     1681 tatgctgact tctttctcct aaaggaatct ttctttgacc aagtgtgagg atacatgcta
     1741 aagagccgaa tccagctgtc gtgtggatga ctgctccacc agcgaaatct ctaaatccca
     1801 tctgatccaa aaatccgcca ccccatatcc agtggcaaac agggaaataa atcagcagat
     1861 tccaaaaaat aacaagtata atatagcctt ttaaattcaa tctccctgca aaagctcctg
     1921 tcatcaaagg aacggtaatc acgataaaca tcagttggta aagaaagaac attaaaaatg
     1981 gaatcgttgc accatgaaac atgttgggga agcttgtaac atgtctcatc aaaaagaaat
     2041 ctgccgggtt tccaataaca ccgccgatat cccgtccaaa ggccaatccg aatccaccaa
     2101 atacccacat aatcgttgtt atcccaacgg agagaaaaga ttgagacatg atcgttagta
     2161 tattattctt ccgtgctaat ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa
     2221 ccattgctgt acataaaatc atgaatgctg tatctcctgt attgataggt aatgtattca
     2281 tttttttcgc ctcctttcta cgattacgag tataaaagaa ggtgtagttg tttttttctt
     2341 ctgttttttt gaattaatac ttgcatttat aaaagacaga caattaataa aagcttgatg
     2401 tgttgtgctc tttttcgttt ttcacacaaa taaagacaaa aaataaacgg tcttcttttt
     2461 atagaaaacc gtttattttc gtcttttttt atttttaaag atattttttc ataaggaata
     2521 aagaatactt catataaaat atataaaaaa gaaccgcagc gagtgtcgct acggttctca
     2581 aatttgttct tcatttcaac ataagttttt cttcaatttg cttataattt acgtgcaaag
     2641 aatgaaacga ctaatactaa aatgattgca ccgatcaatg atggaatgat agccattcct
     2701 gcaattgttg gtccccaagt tcctagtaaa gcttgtccaa ttgctgaacc tactaaacca
     2761 gcaatgatgt ttgcgatgat ccccattgat gatcctttat tcgtgattgc tcctgcaatt
     2821 gctccaatga taccgccgac gattaatgcc caaataaatc ccattttaaa ttcctcctaa
     2881 ctgacttctt acttcttatt gtacacgtgg ttcgttattt tctttgactt tttgtgcacc
     2941 tttgcctaca gcagattttg ctttatctgt ttggcgtgaa gcaaattgtc ctgtggattt
     3001 tgctgcttct gttactttgt cttgtactgt ttcttgatct ttttggtatt cttcttgtgt
     3061 tttgatgtct gcaacattca cattcacttc gataacatct aaatgtgtca tattgtttac
     3121 ttcagtactg atcaattctt tgatttggtt gtaaatatct tcgatgtctt taccgtattc
     3181 tacgatcaca tctagatcca cagctacttg tttcttacct acttctgtat tgatacctgc
     3241 tgttacgtta tctgtgttga ctaatttttc agccatattt gagaaaaatc cgccatctaa
     3301 tgtcaataga ccatctacat ctttcaatgc ataaccgatg attttttgga tgactttgtc
     3361 ttcataagtc aattcaccat tcatcatgcg gttttctcct ttttcatttg ccaaaactga
     3421 tccatttgtt gtattcatgt ttttgttttc catctttgtt tcctcctatg atttaaaaaa
     3481 ttgatcaact attcctgact gtttcaaata aaaaccgatt cctgcgccaa ttgctgtcat
     3541 gattatgatc aacaatgtct tgaaaaatcc aatcgttaac cataaaacag ctagcagtag
     3601 tccaagaccc ccaaagataa tagggagctt gtatgctgaa aatagttctt tcattatttg
     3661 gccctcctat tccactcgag agtgcttgta gtctcttgta tcttcttgtt cttcataacc
     3721 gctgtatgtg actgccactt tgaccgtttc ttttgttccg agtactttag ttacttcttc
     3781 ttcgatatct tgcatcaata cacctgtttt ccctactaat gaagaagttc gtttcaagtc
     3841 tccgttgaca tggatatgga ttcgattttt cgttgaacgt atccgtactt taggtgaatg
     3901 aatgaactct tcttcgtgta aatgggaacg taccaaccct tcgatcgctt ttttgtcgat
     3961 tgttagtttg ccatcttccc gtttcataac gaaagttcct cttgactttg gatagaaaag
     4021 aacgactagc aagatgataa tagcaagaac ggcaaatact acagctgccc agaaaatata
     4081 ttgttgcatg tagtaattcg ttaaagtaaa gaagcgaaat cgttccaatt gttcaggcat
     4141 aatatacggc gcattgatca acgcaatcgc tagaaatact gaaagtagca gtaaactcat
     4201 gataacaccg attattttta accccttatt cattttccac ctcctataaa cttattttta
     4261 ccctgtaaat aactctaccg acaatcacag tatagatagc gaacagttag catagcatca
     4321 gtaaataaag cgctaccaaa cattgctgga tcataacggc gttttattgc cagtctgata
     4381 ctgaaataac aattgcttac ttattctcta tttacttttg attgctggct atacttaaag
     4441 gatattattc aagattaaaa attgcaaaga ttatgctcag ttattattac aagattctat
     4501 ttatttatta aattttttcg gagaattaaa aaatacaata aataacttta gttactttaa
     4561 tgcgttctta ctttattttt atattatcat ttttcttatt cacttctttg gtttatacta
     4621 ttcttataaa taaaaacgag gtatgtccta tgatgaaaag attgtactat tcattgatca
     4681 taacgattgg ctatttgatt gtatcaaatc taggaaatat ggtatttggt atcagtaaag
     4741 aattcagttg gactactaca ttatgggaat ctctattctt tttcatcttc gtatttttgt
     4801 tacaaaatta tcgaaaaaaa taaacatgtg ccatctatct gttggcacat gttttaggat
     4861 ttatggtact ttttattcgg caatccattt tatttttcta cttcttctta atacctctaa
     4921 cacttttgat ttataaacta atccaatgca tctacttgcg cttattaagc tatttcagct
     4981 agatagtacc accccatgcg tcaatgcttg attttccttt tgtataacat tgtataagca
     5041 ataaaggaca aataagtggt gattacacca aaccattgaa taagaaaaat tctgatacgt
     5101 aaataaacat gaacttgaaa aaattaatga atcggttagc tactacgtag ttgtcttttt
     5161 tctcatacag ttctgttaac ttacacttct cttcgctaga taataaaaaa tcctccaact
     5221 gtattgtcca atataatccc aggcatcta
//
LOCUS       DACELF010000125         4809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.132, whole genome shotgun sequence.
ACCESSION   DACELF010000125 DACELF010000000
VERSION     DACELF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.132"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IW687_002530"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="IW687_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0951762.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3252..3770)
                     /locus_tag="IW687_002531"
     CDS             complement(3252..3770)
                     /locus_tag="IW687_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0951763.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1580 a          786 c          730 g         1713 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccactta
//
LOCUS       DACELF010000126         4806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.133, whole genome shotgun sequence.
ACCESSION   DACELF010000126 DACELF010000000
VERSION     DACELF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.133"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..502)
                     /locus_tag="IW687_002532"
     CDS             complement(<1..502)
                     /locus_tag="IW687_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0951764.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIY"
     gene            587..763
                     /locus_tag="IW687_002533"
                     /pseudo
     CDS             587..763
                     /locus_tag="IW687_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(939..1508)
                     /locus_tag="IW687_002534"
     CDS             complement(939..1508)
                     /locus_tag="IW687_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0951765.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1582..1905)
                     /locus_tag="IW687_002535"
     CDS             complement(1582..1905)
                     /locus_tag="IW687_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951766.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2059..2871)
                     /locus_tag="IW687_002536"
     CDS             complement(2059..2871)
                     /locus_tag="IW687_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951767.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3225..4073
                     /locus_tag="IW687_002537"
     CDS             3225..4073
                     /locus_tag="IW687_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0951768.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4218..>4806
                     /locus_tag="IW687_002538"
     CDS             4218..>4806
                     /locus_tag="IW687_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0951769.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSP"
BASE COUNT         1394 a          953 c          852 g         1607 t
ORIGIN      
        1 tataaatccc atcagctctt actctagcta gttcttttcc agtattgtag tccataatga
       61 aaatatactc gtatttataa ttagcaatgt gccatccagc tacatgcaag tttgcgtttt
      121 cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc gtagaatttg
      181 cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt ggattatcat
      241 ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacacct atttcagaaa
      301 gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat aaatgcgatt
      361 tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat ttcattgctg
      421 aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct ttatcatgtg
      481 tgtgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta ctctacatct
      541 tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt cagctagaac
      601 gagacaagca ggaatacctt tagaagatat caaaggtttt ttaggtcata aagatgtttc
      661 cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact
      721 agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt tttaagctca
      781 ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat cactgatttt
      841 ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt
      901 caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg tagaaaaatt
      961 ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc tatgatttca
     1021 gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt cccagtttcc
     1081 atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt aataaaacct
     1141 agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt aatgagcgag
     1201 tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac ggaagctgtt
     1261 ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt cactgtttga
     1321 cgaaggacat ctagttcttg caagagcgtt tcgtattgtt cttgggtcag catcacgcca
     1381 gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc ttgagcaaat
     1441 acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc taatttcttt
     1501 aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga ctgacttgtt
     1561 ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga tatcggtaag
     1621 aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt cgttatggtc
     1681 tagtggaaaa aaatgatcga tgatcactag atctgcatac cgaggcggct tatttcgagt
     1741 acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta ccccagcaag
     1801 tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt caggagggtg
     1861 gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc actctactgc
     1921 ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat ctttggaatt
     1981 gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc ggtcatggat
     2041 accaattacg taacgaagtc attcttggta aagtactttt gtcagctttg atttgctttc
     2101 gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa
     2161 tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa tctggttaca
     2221 gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct gctcctctct
     2281 aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta aaatctcgat
     2341 ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa aggactgctc
     2401 tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag caaaacagca gatatcttac
     2461 ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc
     2521 aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt
     2581 caatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa caaacaagag
     2641 ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat gaagaatcag
     2701 ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg ttttaacatc
     2761 gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca ttagattgat
     2821 tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca tcgatcattt
     2881 ctcacctgat ttctaaacat attgtttgat caaaatgggt tcactcctaa aaagataaaa
     2941 aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta ttttactcaa
     3001 tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata agatcacttt
     3061 gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta tgagaaggtg
     3121 atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag ttgatataag
     3181 aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc gtggccctat
     3241 tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg ttactggtca
     3301 aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact cctgatctgc
     3361 tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag tttgaatttc
     3421 gtccgggacc gatctttacc acgatcttgt tagcagatga gatcaatcgt acgactcctc
     3481 gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc gacaacaata
     3541 cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc gagtacgaag
     3601 gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta caaatcggtt
     3661 atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa gtgttggata
     3721 acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg
     3781 tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca acaagaagtc
     3841 atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt caggcagcaa
     3901 aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg caggatttag
     3961 ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag gaagacgaaa
     4021 agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg tagattgagg
     4081 tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct ggtatatttc
     4141 atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt gttggccttt
     4201 tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa gaaactttcg
     4261 gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc
     4321 atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg cttcccagca
     4381 atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa gaagtccaat
     4441 tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt tttacagcct
     4501 atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt cctgtgactg
     4561 ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg gaaaaaaaac
     4621 agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct tatcgtccag
     4681 gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag tggttatacc
     4741 gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca
     4801 gtccaa
//
LOCUS       DACELF010000127         4784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.134, whole genome shotgun sequence.
ACCESSION   DACELF010000127 DACELF010000000
VERSION     DACELF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.134"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW687_002539"
     CDS             544..1107
                     /locus_tag="IW687_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0951770.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW687_002540"
     CDS             complement(1386..1673)
                     /locus_tag="IW687_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0951771.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW687_002541"
     CDS             complement(1663..1992)
                     /locus_tag="IW687_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0951772.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW687_002542"
     CDS             2297..2626
                     /locus_tag="IW687_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0951773.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW687_002543"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW687_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0951774.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW687_002544"
     CDS             complement(3551..4462)
                     /locus_tag="IW687_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0951775.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1564 a          824 c          722 g         1674 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tctt
//
LOCUS       DACELF010000128         4726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.135, whole genome shotgun sequence.
ACCESSION   DACELF010000128 DACELF010000000
VERSION     DACELF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.135"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW687_002545"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW687_002545"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0951776.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW687_002546"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW687_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0951777.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW687_002547"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW687_002547"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0951778.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACELF010000129         4647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.136, whole genome shotgun sequence.
ACCESSION   DACELF010000129 DACELF010000000
VERSION     DACELF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.136"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1844
                     /locus_tag="IW687_002548"
     CDS             33..1844
                     /locus_tag="IW687_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0951779.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            1905..3365
                     /locus_tag="IW687_002549"
     CDS             1905..3365
                     /locus_tag="IW687_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0951780.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3376..>4647
                     /locus_tag="IW687_002550"
     CDS             3376..>4647
                     /locus_tag="IW687_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0951781.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTIL"
BASE COUNT         1728 a          787 c         1001 g         1131 t
ORIGIN      
        1 ttttgaaaga ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat
       61 caaaaaaata ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag
      121 aggctgcgcc aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat
      181 tcaacgaaaa tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag
      241 aattgatcga tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata
      301 aattctattt tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag
      361 gggatggcgt caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag
      421 tacctgaaaa ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc
      481 agtcgaaaga aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta
      541 agcaagaagt caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt
      601 ctcctgaaga cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta
      661 gaaatcaatt attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg
      721 cgttgaaaaa agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca
      781 gaaaaacggt caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta
      841 caccagaaga taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact
      901 acacgattga aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga
      961 aaatctttcc gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc
     1021 aagctgggat tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa
     1081 gtgaagtcta tcaaagagaa ttaggacaag cacggggggc gtatgttcgt aacgaattta
     1141 ctggcgaaga agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa
     1201 gtattcatga attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta
     1261 aagaattgca ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt
     1321 cggagaaagc tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata
     1381 aggaagcggc ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca
     1441 ttcaaaccat ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag
     1501 cagaagtgaa gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc
     1561 ccgttggttc actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc
     1621 gaacgttaca tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt
     1681 taaaaggaaa aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc
     1741 taggaaatcc attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg
     1801 aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac
     1861 tatctgaaaa acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa
     1921 tgagtcttat tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa
     1981 tggctgtagt caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg
     2041 atcgaagaaa acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat
     2101 tagctaaact ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag
     2161 ccacagattt tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg
     2221 agacagaaaa gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga
     2281 attatctgaa ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag
     2341 ggttgattgc cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt cgagacgatc
     2401 gtttagatca tgcggtcgcc tttttatgga aagatccaca gacaaatgag acagtagggg
     2461 cgagttatca aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc
     2521 atatagataa aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc
     2581 agttaaaatt ttttgagagt agtattgatt tacttagcta tgctgcacta aatcgtgatc
     2641 agttaaacga tacgtggtta gtttcgatgg aagggttaaa acaccatgtg atcagtcatt
     2701 attttggtga ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga
     2761 tttgtgtgga taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg
     2821 cagttgatcc atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc
     2881 aagtgccaaa agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa aagatcacac
     2941 cagaagctat tatggccata cacaagacag aaaacaatct gcaattaacg aaccaattgg
     3001 tttccgctca caaagtaaat gcgtcttttg gtcagcagct ttctgttaac gattcaatag
     3061 aagctattaa tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gcttgtgaac
     3121 gcgtggatgg tacgtacgat tttgatcgtt tttatcaaga aaaaggggat attaatgctc
     3181 agattctttt ctcttataaa gcagaacaat actacaaagg ctataaaaat catgaacatg
     3241 aatttgtacc tgaagtaaaa aaggattgga atgatcaatt gaagcatgaa atctaccaac
     3301 aagagatcag aaagcaaaaa cgtgccatgt tgtttcaaca aggtagacag caagaaaggg
     3361 agtgaaatcg tcatgatgaa aaccgttatt ttagcagaaa aaccgtcaca agcgaaggct
     3421 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
     3481 ttgttttcag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
     3541 cccgatatgt acgatgaaaa ttggaaacaa tggtcattag agcatttgcc tattttccct
     3601 acacattatc actatcatgt tcctaaagat aagaaaaagc aattcaaggt ggtgaaacag
     3661 caacttcaat cggccgacac cattattatt gcgacagata gcgatcgtga aggggaactg
     3721 atagcttgga cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaagactt
     3781 tggatcaact ccttagaaaa agaagccatt tatcagggat tccagcagtt acgagatgca
     3841 gaagagacct acccaaaatt tgaagaagca caagcacgcc agatcgccga ttggttgatc
     3901 ggcatgaacg gtagcccgct gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     3961 ttttccttag gcagagtcca aaccccaacg ctttacatga tttatcaatt acaggaaaag
     4021 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     4081 ggcgcatttg atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa
     4141 gattatttaa aagaaaaggg ggttcaattg ggaaaacagc caggtacgat ccaccaagtt
     4201 gaaaccgaga aaaaaagtgc ggcaagtcca cgtctttttt ctctatccag ccttcaatct
     4261 aaaatgaatc agctcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     4321 tatgaaggga aatatctaag ctacccacga acagataccc cttatattac agaaggggag
     4381 tatgcgtatc tgttggatca cttagacgaa tacaaacact ttttaaaggc cgaagccatt
     4441 cctactccta ttcatacacc gaatagtcga tatgtgaata ataagaaagt ccaggaacat
     4501 tacgcgatca ttcctacaaa gacggtcatg acggcagcgg cttttgaaca attaagtcca
     4561 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     4621 tatacctatg aagaaacaac gatcttg
//
LOCUS       DACELF010000130         4572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.137, whole genome shotgun sequence.
ACCESSION   DACELF010000130 DACELF010000000
VERSION     DACELF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.137"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(338..1291)
                     /locus_tag="IW687_002551"
     CDS             complement(338..1291)
                     /locus_tag="IW687_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0951782.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="IW687_002552"
     CDS             complement(1314..2069)
                     /locus_tag="IW687_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0951783.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="IW687_002553"
     CDS             complement(2066..3025)
                     /locus_tag="IW687_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0951784.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="IW687_002554"
     CDS             complement(3018..3968)
                     /locus_tag="IW687_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0951785.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="IW687_002555"
     CDS             4118..4570
                     /locus_tag="IW687_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0951786.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1491 a          962 c          775 g         1344 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa aa
//
LOCUS       DACELF010000131         4420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.139, whole genome shotgun sequence.
ACCESSION   DACELF010000131 DACELF010000000
VERSION     DACELF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.139"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            63..917
                     /locus_tag="IW687_002556"
     CDS             63..917
                     /locus_tag="IW687_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0951787.1"
                     /translation="MENSLYLVKEPNTHLRDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINEQKYYLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGELVEYY
                     LKEIGVGKENLTFKVENLNSFKGIIFETLSYDQDNLISELSRQTQVYKFLELLLSSAS
                     YLKEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAIKLSIHENYLSRLFKSDMGISI
                     KEYISEIKLSIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGAYRKRRL
                     GLGIINVE"
     gene            1014..3263
                     /locus_tag="IW687_002557"
     CDS             1014..3263
                     /locus_tag="IW687_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0951788.1"
                     /translation="MYKNNEMNIIYRSENQQFHLFNQEISYIFKISDDGKLLHLYYGE
                     NLPDNDYTHLIEMHHRPMTTYRKENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLTDFIYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYVLDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLLADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3266..>4420
                     /locus_tag="IW687_002558"
     CDS             3266..>4420
                     /locus_tag="IW687_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0951789.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVIVFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLANINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          809 g         1429 t
ORIGIN      
        1 ttatgtaata atataaacat gatgaaacaa aaattatcca aatctaactt aggaggacta
       61 atatggaaaa ttctctatat ttagtgaaag agcctaatac ccatttgaga gattgttact
      121 ttaatttttg tggatactca aaaacagaac ctagacatag ttttggacca gctattcgag
      181 aacagtactt aatacacata gtcttagaag gtgaaggtta ttattcaatt aatgaacaaa
      241 aatactatct gaaaaaagga caaggttttg tgattccacc aggagtatct actttctatc
      301 aagcagatga aaaaaatcct tggtcgtatg tatggttggg attaagtgga gaacttgtag
      361 aatactatct aaaagaaatc ggtgttggaa aggagaatct tacctttaaa gtcgaaaact
      421 taaattcttt taaaggaata atctttgaaa ctttatctta tgatcaggat aatctaatta
      481 gtgaattatc cagacaaaca caagtttata aatttttaga actcctcttg agctcagcga
      541 gttatttgaa ggaagggata attactaaaa agatgaatcc ttttgtacgt caaacaatag
      601 aaataatccg tgagaatgct tatcaaaagt tatctgttaa agatattgct ataaaactgt
      661 cgatacacga aaattattta tcacgattgt ttaagagtga tatgggaata tcaataaaag
      721 agtatatcag tgaaattaag ctttcgattg caaatgacct actatcatca actgaatatt
      781 caattcagca aatcagtgat aagataggct tttcgacagt tcaatctttt tctaaagctt
      841 tcaagaaggc caatggtgtt tcacccgggg cgtatcgaaa acgtaggtta ggtcttggaa
      901 ttattaatgt tgaataaact aaacatttga ttttgataaa tattcgttac tttaaacgct
      961 tactatttat gtaagcgttt tttataatgc aataaagaag tggaggagag cgtatgtata
     1021 agaataatga aatgaatatt atttatcgat cagaaaatca acaatttcat ttgtttaatc
     1081 aggagataag ttacattttt aaaatatcgg atgatggtaa attactgcat ttatattatg
     1141 gtgagaactt accagacaac gattatactc atctaattga gatgcaccat cgacctatga
     1201 ctacttatag aaaggaaaac gatttattat attccctaga gcacttgaaa caagaatttc
     1261 ctgaatatgg tacaacagat tttagacatc ctgccatttc actactacag aaaaatggct
     1321 ctcgtctaac agattttata tatcaaggat acgaagtaga agaaggaaaa ccaaaattag
     1381 aaggtttacc tgctacctat gttgataata atgatgaaag caaaacattg aaagtttatg
     1441 ttttagattc agttacaaat gttcaagtag aattattata tacaatttat agagattatc
     1501 ctgttattac tcgtagcgcg cgagttatga ataaaggaaa agatattcaa ataatcgaga
     1561 atattagcag tttatcattg gatttacccg atgcaaatta tgaatggcaa caattatctg
     1621 gtgcttgggg aagagaaaga actattaagt cgcgtacttt acaacaagga attcaatcaa
     1681 ttgaatctac tcgaggtatt tcaagccatc agcataaccc atttgttact ttaaaaagaa
     1741 aaaatactga tgaaaatcaa ggagaagcta ttggtgcagc actagtttac tcaggaaact
     1801 tcttaattca agcagaagta gatacatggg atgttactag actacaagtt ggcatcaatc
     1861 catttggatt taattggaaa ctcaatccag gagaaatatt tacagcacca gaagcgattc
     1921 tggtttattc aagtaagggt ttaaatgcca tgagtcaaac ttttcatcaa ctatttagaa
     1981 aacgcttagc tcgtggtaag tggcgagaaa aagaccgtcc tgtcttaatt aataattggg
     2041 aagcaacata ttttgatttt gacgaagaaa agttggtaaa tattgctaaa aaagcatttg
     2101 atgttggtat tgaattattt gtattagatg atggctggtt tggagaaaga gaaaacgatt
     2161 gtgcaggatt aggagattgg catgtaaatc ctaaaagact acctagagga atcggcagcc
     2221 tttcagaaaa aattcgtagt tttggtatga aatttggttt atggtttgaa cctgaaatgg
     2281 tgaataagga tagtgagtta tatcgcacac atccgaattg gattatttct acacctaatc
     2341 gaaaacctaa tcatggtagg aaccaatatg tattaaattt tgggttagat gaggttgttg
     2401 aaaatatttt cgatcaaatg tgtaaaatca ttgatgaatc aaacttagat tacattaagt
     2461 gggatatgaa tcgtccattg actgatgtat ttgacagtca cttgttagca gatcaacaag
     2521 gagaagtatt ccacagatat gtactaggcg tatatcgctt atatgaaaaa cttataacaa
     2581 aatatcctaa catactattt gagtcttgtt catcaggtgg tgggcgcttt gatcctggaa
     2641 tgcttcacta tgcacctcaa gcttgggcta gtgatgattc agatgcaatt gaaaggttaa
     2701 aaattcaata tggaactagt atgctgtatc ctctttcgtc tattggtgct catgtatcaa
     2761 ttgttccaaa tcatcaaaca aaccgtataa cacctataaa aactagagga aatgtagctt
     2821 tttttggtgc atttggatat gagcttgact taaatgaatt aagtgaaggc gatttgagga
     2881 ttgtaaaaga acaaattgcc ttttataaaa aatatagaac cataattcat aatggtactt
     2941 tttatagact attatcacca ttcgatgatg ataatcaaac cgcgtggatg gttgttagtc
     3001 atgataagaa aacggctatt gttgctcatt ataaaacatt aaatgaggta aatgctcctt
     3061 accgccgact gaaattaaaa gggttagata atgacggact ttataatgta ggacaaaata
     3121 gtttgcgatc tggaatggaa ttgatgagag caggactagt ctgttcagat gcatctagtg
     3181 gacaaataaa tgatcagaat cgacgcccag aaacatttga ttttgattcc aagatttggg
     3241 ttataacaca ggaggaaaag tagagatgaa taaccaaaaa agcttacttc aaaaaggagc
     3301 aatcttaagt atttcactaa tgttaacaag ctctcaagca attaatggtg ttattccaca
     3361 aatgaaacag tctttgggga tatcacaaag tcaaagtgaa cttttaggaa ctgcaccaag
     3421 tataactgtt attgtgttta tcttattatc tagttacttt gccaaaagaa taggaatgaa
     3481 aaagacaata attttaggtt tattattagc tggtatagga ggaattattc cagtatttac
     3541 agctaccttc caaaccgttc ttattagtcg agtaatatta ggtgccggtt taggagtata
     3601 taattcattg gctgtgacat atatcagctc tttatactct ggcaatacta gagcaacgct
     3661 attaggtatt agaaattcaa tggaagcttt aggtcagaca atccttattt ttcttgctgg
     3721 agttttagcg aacattaatt ggacggcttc gtttttagtc tatgcaattg cgtttcctgt
     3781 agctctctta ttcgcgctaa aagttcctga aattagagat gaaaatagtg acatacctga
     3841 aaatcatgta aaagaaaaga tgaatccaat ggtttttgcc ctagttctat ttgcaatctt
     3901 actcgtaatg aatagtattg ctatttctgt acgatttgca tcaattgcaa ctgaaattaa
     3961 aggggtaaac tttaatgcta gtaactattt agctctaatg cctatcttag gaatactagc
     4021 aggatttatg tttggaccaa taaataagtg gataggaaaa ggaaccctat acttgggaat
     4081 tatattcttt ataatttcaa atttgttaat tgctgtgtca aacggtaata tgacgttcct
     4141 gctaacaggt ttatttttat caagtataac aggatcatgg tgcttcccat ttatttttag
     4201 taacttagat aaagtcacta caaagaatac aattaatttt gctacttcat tgatttttat
     4261 tggctgtaat attggtaatt ttatagctcc tattgctatg caattagcac aatttctaac
     4321 aagaagtaca aatttgactg ctccattttt tgtattcgca ggaatattta ttgtgatcct
     4381 atttgtaaca ttttttgcga caaataaaaa aaatactaga
//
LOCUS       DACELF010000132         4401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.140, whole genome shotgun sequence.
ACCESSION   DACELF010000132 DACELF010000000
VERSION     DACELF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.140"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..1619)
                     /locus_tag="IW687_002559"
     CDS             complement(99..1619)
                     /locus_tag="IW687_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0951790.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1715..1840)
                     /locus_tag="IW687_002560"
     CDS             complement(1715..1840)
                     /locus_tag="IW687_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0951791.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(1862..2047)
                     /locus_tag="IW687_002561"
     CDS             complement(1862..2047)
                     /locus_tag="IW687_002561"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0951792.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="IW687_002562"
     CDS             complement(2031..2213)
                     /locus_tag="IW687_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951793.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="IW687_002563"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="IW687_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="IW687_002564"
     CDS             3167..3766
                     /locus_tag="IW687_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0951794.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4401
                     /locus_tag="IW687_002565"
     CDS             3830..>4401
                     /locus_tag="IW687_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951795.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1403 a          799 c          785 g         1414 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag a
//
LOCUS       DACELF010000133         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.141, whole genome shotgun sequence.
ACCESSION   DACELF010000133 DACELF010000000
VERSION     DACELF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.141"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW687_002566"
     CDS             complement(83..1051)
                     /locus_tag="IW687_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0951796.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW687_002567"
     CDS             complement(1498..2904)
                     /locus_tag="IW687_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0951797.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW687_002568"
     CDS             complement(3126..3488)
                     /locus_tag="IW687_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951798.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW687_002569"
     CDS             complement(3492..3800)
                     /locus_tag="IW687_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951799.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW687_002570"
     CDS             complement(3809..4228)
                     /locus_tag="IW687_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951800.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW687_002571"
     CDS             complement(4221..4361)
                     /locus_tag="IW687_002571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951801.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACELF010000134         4266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.142, whole genome shotgun sequence.
ACCESSION   DACELF010000134 DACELF010000000
VERSION     DACELF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.142"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1485
                     /locus_tag="IW687_002572"
     CDS             <1..1485
                     /locus_tag="IW687_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0951802.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            1589..1813
                     /locus_tag="IW687_002573"
     CDS             1589..1813
                     /locus_tag="IW687_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951803.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            1826..2329
                     /locus_tag="IW687_002574"
     CDS             1826..2329
                     /locus_tag="IW687_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0951804.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            2389..2778
                     /locus_tag="IW687_002575"
     CDS             2389..2778
                     /locus_tag="IW687_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0951805.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2765..>4266
                     /locus_tag="IW687_002576"
     CDS             2765..>4266
                     /locus_tag="IW687_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0951806.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         1427 a          696 c          919 g         1224 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atcaggaact tacctatcca tatatcaatg
     1621 gtttttaacc aatgcccaat ccttagtctt actggcaatt gtggtcattg gtttgttttt
     1681 aggatttaag cgggagtttt ccaaactgat tggctttttg gtgattgctt tgattgcggt
     1741 cggattggtc tttaatgctt ctggggtgaa ggatgtctta ctcagtttgt ttaaccgaat
     1801 tgtcggtgcg taagggggaa gcacgatgga acagatgcgt gtttatattg cgaacctcgg
     1861 gaaatataac gaaggtgaat tggtgggggc gtggtttacc ccacctgtcg attttgatga
     1921 agtgaaagaa cgaattgggt taaatgacga atacgaggaa tatgcaattc atgattatga
     1981 acttcccttt gagattgatg agtatacgcc aattgaggaa attaatcggt tgtgtggctt
     2041 agcggaggaa cttgaaggaa cgccagttgg tgaagttgct tctgagattc aacatgcttt
     2101 cttcaattct tttgaggaga tggtagagca tgtggatgat attatctatt atccagattg
     2161 cgatgatatg agtgatgtag catatttctt gattgtagaa gctggtgatt ttggagaagt
     2221 accagaacac ttgaagacgt atattgattt tgatgcgtgt ggtagagact tggagattgg
     2281 aggcaattat ttggtaacga gtcgaggtgt ttttgagtat ccatattaac aaaattattg
     2341 aagggtagtt gatttctaaa ctatccttct ttttaaagga atgatttgat gaagaaaata
     2401 aaaagctaca ccagtatttg ggcagttgaa aaggtcattt atgcaattaa tgactttcaa
     2461 ctgccttttc ctgtgacttt taaccagatg gcttggtttg ttttatcctt gctgtttgtc
     2521 atcgtatttg cccatgtgcc tccactatct atgatagagg gggccttttt aaaatactta
     2581 ggtataccgg tggcagttac ttggttcatg tcgcaaaaaa cgtttgacgg taagaaacca
     2641 ttgggatttt tacgatcttt tattagctat cacttacgat ttaaggtcac tttcgcagga
     2701 aagaaagtca aagaacagaa gaaacgatgg gacgaaccca ttactctggt aaggagtgtg
     2761 aactatgtac ccgattaagt atattgaaaa taatcttgtc tttaatcaag agggagaatg
     2821 ttttgcctac tatgagttaa taccttacaa ttactccttt ttgtcacctg aacagaaata
     2881 tcaagtccac gataactttc gtcaattgat tgcgcaaaat cgtgaaggca agattcatgc
     2941 cttacaaatt gccacagaaa gtagtattcg tgccacgcaa gaacgatcaa aaaaggaaat
     3001 taccgggcga ttaacagaag tagcgaaaca acgaattgat ttacagacag aggcattagt
     3061 atccatgatt ggcgatagtc agattgatta tcgctttttt attggcttca aactgattgc
     3121 gacagatgaa gaggtcaatc taaagaatct caagaaatct ttcttctccg gctttcaaga
     3181 atttgtctat ggagtgaatc atcatttgat gggcgatttt gtttccttat ccaatgaaga
     3241 aattcgccga tataccaagc ttgaaaaact aatggaaagt aaattggctc gaagatttaa
     3301 agtcagacga gtcacaccga gtgatctgat gtatttgatt gaacatatct atggcgaaaa
     3361 gggtacacca tttgaagaat atgaatttca gctaccaaag aaaaagctga agtcggaaac
     3421 tttggtgaaa cgttacgact tattacgtcc cagtcgttgt ttgattgaag aaaagccccg
     3481 gtacttatgc atggaacatg agaatcatga atcatatgta gcgtacttaa caattaatac
     3541 aattgtgggg gaaatggagt ttccctcctc agaacttttc tattaccagc aacaacaatt
     3601 tacattccca attgacacta gtatgaatgt ggagattgtg acgaacaaaa aggcacttgc
     3661 taccgttcgc aataagaaaa aggaattaaa agacttggac aaccatgcct atcaatctga
     3721 caatgaaaca aattccaatg tcttagatgc attggattcc gtagatgaat tggaaactac
     3781 cttggatcag tccaaagaat ccatgtacaa actgagctat gtggttcgag taagtgccga
     3841 atcagtagat gaactaaagc gccggtgtga tgaagtgcta gatttttatg atgataccaa
     3901 tgtaaaacta gtccggcctt ttggcgatat gatggggcta catgaagaat tcttaccttc
     3961 tagtaagcga tacatgaatg attatatcca gtatgttacg tctgattttc tcgccggact
     4021 tggttttggc gccactcaga tgttagggga gttggaaggc atttactttg gttataacgt
     4081 agatacgggg cgcaatgttt acctgaaacc ggcattggcc tcacaagggg tcaagggttc
     4141 agttaccaat gcgctagcag cggcattcct cggttcactc ggtggcggaa agtcctttag
     4201 taacaatctc ttagtttatt acgccgtcct ttttggtgga caagctgtga ttgtcgatcc
     4261 aaaagg
//
LOCUS       DACELF010000135         4253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.143, whole genome shotgun sequence.
ACCESSION   DACELF010000135 DACELF010000000
VERSION     DACELF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.143"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..607)
                     /locus_tag="IW687_002577"
     CDS             complement(77..607)
                     /locus_tag="IW687_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0951807.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            712..1263
                     /locus_tag="IW687_002578"
     CDS             712..1263
                     /locus_tag="IW687_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0951808.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1416..1889)
                     /locus_tag="IW687_002579"
     CDS             complement(1416..1889)
                     /locus_tag="IW687_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0951809.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2027..3499
                     /locus_tag="IW687_002580"
     CDS             2027..3499
                     /locus_tag="IW687_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0951810.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3496..4206
                     /locus_tag="IW687_002581"
     CDS             3496..4206
                     /locus_tag="IW687_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0951811.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1329 a          763 c          829 g         1332 t
ORIGIN      
        1 atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct gatttcacaa
       61 cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat tttccgctac
      121 ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca ttgtctatac
      181 ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt ggataagttt
      241 catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa gtaacagtga
      301 tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt ttcttttcat
      361 ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg attttgaata
      421 cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg cctttgacca
      481 ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat tcatattccc
      541 aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat tctttaaatt
      601 ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca tgttttcgat
      661 tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact gatggcaaga
      721 aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat gacaggatat
      781 gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa acatagtcag
      841 atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca agtagagatc
      901 acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa ggaacaattg
      961 gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga atttgttctg
     1021 aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat tcagcagcag
     1081 cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac gatctttcct
     1141 actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca tgcgatcaat
     1201 cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa ccgaaaaaaa
     1261 taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa cgcagatcac
     1321 cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta gcatctactt
     1381 attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa caactgttcg
     1441 tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt ctcgttgtca
     1501 tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa aatcgtatag
     1561 cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg gacatggtgc
     1621 attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa tccgtcatct
     1681 aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt tctaggacaa
     1741 ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct taattgacgt
     1801 ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg atcattttcc
     1861 aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct cttgcttccc
     1921 ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga atttaggact
     1981 aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga gtaacaacca
     2041 acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg tcttattaat
     2101 tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc ctacattgat
     2161 gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt tcttgctagt
     2221 caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt caagaaattt
     2281 atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta cagcacctaa
     2341 ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag tatcgatgcc
     2401 attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat cagcaatggg
     2461 gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat ctggttatat
     2521 cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg ttgttcttgt
     2581 cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc ctagcattga
     2641 tattttatcc gctattttat caacgattgg attcggtagt ttactttacg gattttctag
     2701 tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg ttggtgtgat
     2761 tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct tggaacttcg
     2821 tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa caaatatggc
     2881 gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg gagaatcagc
     2941 attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta tgatgcctgt
     3001 gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag ggatgttcat
     3061 tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag ttttatatat
     3121 cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc cggtaacgac
     3181 ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg taaataatac
     3241 attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt tgacgaatgt
     3301 gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag cttataaaga
     3361 tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag cgatcatctt
     3421 tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg ttctaatgaa
     3481 agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc ttttgcttta
     3541 acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt cggactaatc
     3601 ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg aatggaaaaa
     3661 gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg agcagatatg
     3721 cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg aacaaatgaa
     3781 aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt agaaaagaca
     3841 gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa cgacatgtat
     3901 aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt gaatacattc
     3961 aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt agctgaatta
     4021 gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca agacggtatg
     4081 gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca acgtacgaaa
     4141 gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga agcaaaaggc
     4201 gaataatatt ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aac
//
LOCUS       DACELF010000136         4062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.144, whole genome shotgun sequence.
ACCESSION   DACELF010000136 DACELF010000000
VERSION     DACELF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.144"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            580..1269
                     /locus_tag="IW687_002582"
     CDS             580..1269
                     /locus_tag="IW687_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0951812.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1256..2464
                     /locus_tag="IW687_002583"
     CDS             1256..2464
                     /locus_tag="IW687_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0951813.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2758..4029
                     /gene="serS"
                     /locus_tag="IW687_002584"
     CDS             2758..4029
                     /gene="serS"
                     /locus_tag="IW687_002584"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0951814.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1319 a          701 c          808 g         1234 t
ORIGIN      
        1 cgattcgttt ttggttgccg tagcaacttt tatatcatat cagagagaca agtaattgtc
       61 aactcttttt taaaagttct ttcgatttcg tttttcgaaa tcttccagca actcatttat
      121 cataacttat cgtttgttat ttgtcaagaa cttttttgaa gtttttttcg atagaatcaa
      181 gcttcgcaag tggttctttt ttaacgactc agttatctta tcatgtcatt tccaacatgt
      241 caacaactta ttttttatta tttaataagt tattggtgtt cttttgaaca aataataata
      301 tatcaacatc acatgtgaaa gtcaataaga aaatgcttat ttatcaacat ttctagctta
      361 catgatcatt ggttagttct ggtccttttt cgttcgtctt tataactccg agagacgtaa
      421 aatccttttg ttttcagttt aatgttcatg ttcttgtctt attcacacca actagatgtc
      481 tcaccttcta aaattttcct taacaaagtg ctgcttttat ctatttctgc ttttttttgc
      541 tagaataata gtaacaatat gactctggag gatttattta tgaaaatttt gattgctgac
      601 gatgataaag aaatcgttga attattaagt atctatgttc acaatgaggg gtatgaagca
      661 gtaaaggctt atgatgggaa agaagcatta tctaaaatca gaacaatccc tgatattgaa
      721 ttacttattt tagatatcat gatgcctgag atggatggga tgcaagtagt gaaagagcta
      781 cgaaaagaat cccaaatacc aatcattatg ctgacagcaa aaactactga tatggataag
      841 atcaaaggac tggtcgctgg tgcagatgat tatgtgacca agccatttaa tccgttagaa
      901 gtaatggcta gagtaaagtc tttattacgg cgtacaaaaa tgcagattgc cccagatcag
      961 cctgatgtat tagaagtaaa tgcattaatc atcaataaag actctcatga agttaaaaca
     1021 atcgatggga aagaaatcca gttaacagct ttggaattcg gtatcctcta cttgcttgct
     1081 agtcatccta atcgagtatt tagcgcggat gaaatttttg agcgtgtttg gaaacaagaa
     1141 agtattgtct ctgctaaaac tgtcatggtt cacgttagtc atttaagaga taagattgaa
     1201 gaagcaactg gtggtgaaaa agtcattcaa accgtttggg gagtgggcta taaaattgac
     1261 gcaagatagt aaacgagcaa ctgaaaaacg ccgtcggatc aatttgactt caaaagaaat
     1321 cagcgaattg ttagcggaag gaatcatcac gattatcctt ttgcttctat taaatgtttc
     1381 tattctggtt gttatcagtt cggtgatcaa cagttcacct tctttgacga atgcgatttg
     1441 ggattcaaag aatatttttg cagaacgatt gaacactgat ttgttctgga acggccggaa
     1501 ttttattatt ccctttttct ttctattaga tattggtgtt ttgtattggc gcttgattcg
     1561 aagatatcgc caaatgcaat tgcggcatat catcagcgaa cttcactaca tcgcaaacgg
     1621 aaattatgat catcgtatcc cttttgaact gagtggtgac ttaagccgag tggtaacaag
     1681 tatcaatgga ttggtggata gtaccgtagc agcgattgaa gacgaacgta aaatcgaaaa
     1741 atcaaaagat gaattgatca cgaatgtcag tcatgatatc cgtaccccac tcacgtccat
     1801 cattggatac ctaggactga tagaagatgg ccagtatcac tcagaagaag atcttttaaa
     1861 gtatacgcat actgcttata tcaaagcaaa gcagatgaaa tctttagtgg acgacttatt
     1921 tgaatacact aaggtacgcc agcctgctgt cccggtgaat ttttctgcct ttgacatgat
     1981 ccagttgatc gaacagttag cagctgattt tgagttggaa gcttctaaaa aaaatatcca
     2041 aatacttgtt caatcaaaag tagactccct tatcatggat ggcgataccg aaaaacttgt
     2101 acgtgtcttc aataatcttt tgacgaatgc gttgaaatac ggaaaaggag caacgaagat
     2161 cgtaatcgaa gtagaacgta tcggttcaga agttgttgca acagtcaaaa ataacggagc
     2221 aatgatccca caacaggcaa tcgataatct attcgaccgt ttttatcgtg tagaagaatc
     2281 tcgttcacaa gcaactggag gaactggact tggtttagca atcgctcaaa gcattgtggc
     2341 tttgcacggc ggatacattt atgcaaaatc agataagcaa tggacttctt tcatcatgca
     2401 ccttccaatc aagaaaaatg aaaaattacc tattgataca caggaagtta ttgacgaaag
     2461 ctgaaatatt cgctattctt attacaatta ttagaataaa tgaaaaacac agaacccagt
     2521 acactttatt ctttttcaga gagctgatgg caggtgtaaa tcagcaaagg ataatttgga
     2581 agttccagtt cgtttacgtg ccctttcgaa ataagaaagg tcggttcaac agccgttatg
     2641 tgttcaacaa gtgcagctga cgctgaactt gggtggtacc gcgaatttca gactgattcg
     2701 tcccaagaat aatgggatgg atcagtcttt ttttattttc aaaaaggagg aaatcatatg
     2761 ttagatgtaa aaatgatgcg acaaaatatt gaagaagtac aagaaaaatt gatgactcgg
     2821 ggcgtaaaaa aagaaacatt agctgaattc atggaactag atgaaaatcg tcgtaagcta
     2881 ctagtcaaaa gtgaagaatt aaaaaaatat cgaaatgatg tttctgctga gatcgcccaa
     2941 ttgaagcgtg caaaagaaga tgcttctgct aaaatcgctg aaatgaaaga agtcggtggc
     3001 aacatcaaag cattggatac tgaaattgct gaagtggatg aaaaattgaa acaaattgca
     3061 actactttgc caaaccttcc ccatgattct gtgccagttg gaacagatga agaggacaat
     3121 gtagaaatcc gtcgttggag cgaaccacgt acatttgctt ttgaaccaaa accacactgg
     3181 gaaattgcag aaaatcttga tattttagat tttgaacgtg gcgcaaaagt agccggtagc
     3241 cgttttgtct attacaaagg attaggtgct cgattagaac gtgctgtcta caacttcatg
     3301 cttgatcagc atatttacga acacggctat actgaaatga tcactcctta tatcgtcaac
     3361 agtaaagcga tgtttggtac gggacaattt ccaaaattca aagaagatgt tttccaattg
     3421 gagaatactg atctaacatt aatcccaaca gctgaagtac cattaactaa ctattataat
     3481 gatgaaattt tagatggtgc agatttacct atttatttca ctgcactaag ccctgctttt
     3541 cgttctgaag ccggaagtgc tggtcgggat acacgaggac tgattcgtct tcaccaattc
     3601 aataaagtag aaatggtcaa attttcggat gtagagcatt cttacgaaga attggaaaaa
     3661 atgacagctg atgcagaaga tatcttgaaa aaattgcatt tgccttatcg tgtgatggcc
     3721 ttatcaactg gagacatggg cttttctgca gctaaaactt atgacttaga agtatggata
     3781 cctgcacaag atacttatcg tgaaatcagt tcttgttcta actgtgaaga tttccaagca
     3841 cgccgcgcaa tgatccgtta tcgcgataat gatggaaaaa tccactatac tcatactctg
     3901 aatggttcag gacttgcagt tggccgtaca gtagctgcca ttttggaaaa ctaccaaaat
     3961 gaagatggtt ctgtcacagt tccagaagcg ctcgttcctt atatggggaa tctgaaagtt
     4021 attaaataaa gaggctgtga aaaagccgct tagtttcgag ta
//
LOCUS       DACELF010000137         4016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.145, whole genome shotgun sequence.
ACCESSION   DACELF010000137 DACELF010000000
VERSION     DACELF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.145"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW687_002585"
     CDS             complement(176..1462)
                     /locus_tag="IW687_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0951815.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW687_002586"
     CDS             complement(1455..2723)
                     /locus_tag="IW687_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0951816.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW687_002587"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW687_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0951817.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW687_002588"
     CDS             complement(3495..3941)
                     /locus_tag="IW687_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951818.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1137 a          817 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatc
//
LOCUS       DACELF010000138         3992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.146, whole genome shotgun sequence.
ACCESSION   DACELF010000138 DACELF010000000
VERSION     DACELF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.146"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..2549)
                     /locus_tag="IW687_002589"
     CDS             complement(57..2549)
                     /locus_tag="IW687_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0951819.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2556..3023)
                     /locus_tag="IW687_002590"
     CDS             complement(2556..3023)
                     /locus_tag="IW687_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0951820.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3159..3232)
                     /locus_tag="IW687_002591"
     tRNA            complement(3159..3232)
                     /locus_tag="IW687_002591"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3196..3198),aa:Arg,seq:acg)
     gene            complement(3290..3363)
                     /locus_tag="IW687_002592"
     tRNA            complement(3290..3363)
                     /locus_tag="IW687_002592"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3327..3329),aa:Pro,seq:tgg)
     gene            complement(3385..3458)
                     /locus_tag="IW687_002593"
     tRNA            complement(3385..3458)
                     /locus_tag="IW687_002593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Arg,seq:acg)
     gene            complement(3469..3554)
                     /locus_tag="IW687_002594"
     tRNA            complement(3469..3554)
                     /locus_tag="IW687_002594"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3518..3520),aa:Leu,seq:taa)
     gene            complement(3571..3642)
                     /locus_tag="IW687_002595"
     tRNA            complement(3571..3642)
                     /locus_tag="IW687_002595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3608..3610),aa:Gly,seq:gcc)
     gene            complement(3654..3726)
                     /locus_tag="IW687_002596"
     tRNA            complement(3654..3726)
                     /locus_tag="IW687_002596"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3691..3693),aa:Thr,seq:tgt)
     gene            complement(3744..3825)
                     /locus_tag="IW687_002597"
     tRNA            complement(3744..3825)
                     /locus_tag="IW687_002597"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3789..3791),aa:Leu,seq:tag)
     gene            complement(3833..3905)
                     /locus_tag="IW687_002598"
     tRNA            complement(3833..3905)
                     /locus_tag="IW687_002598"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3870..3872),aa:Lys,seq:ttt)
     gene            complement(3909..3981)
                     /locus_tag="IW687_002599"
     tRNA            complement(3909..3981)
                     /locus_tag="IW687_002599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3946..3948),aa:Val,seq:tac)
BASE COUNT         1080 a          923 c          701 g         1288 t
ORIGIN      
        1 acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc aagtaactat
       61 agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt accttttgaa
      121 gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc ctcgctcaat
      181 cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata ttcaggatca
      241 aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg aatatcttgt
      301 tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg gatttcatct
      361 ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc aggtcggaaa
      421 gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt agtaacatct
      481 tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat atttgaagtc
      541 ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt taaatgacca
      601 tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc cacttcatct
      661 aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc accttcatca
      721 taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt ttccataaat
      781 tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc tgaaagtgct
      841 ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga accaattgga
      901 cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac tgctttgaca
      961 gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa caagcgttcg
     1021 ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac aactgtagca
     1081 acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc aattgcagaa
     1141 actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc agcattttca
     1201 aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat ttctagtttg
     1261 atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc tgctgattca
     1321 tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg aacagaaaga
     1381 ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc ttcatatctt
     1441 gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc tacttgaatg
     1501 cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata ttcatctagg
     1561 gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa gatattcgaa
     1621 gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc atcaatgaat
     1681 aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg atcttcaaat
     1741 tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat caggcgcttt
     1801 tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc ttcagcaatg
     1861 gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt ccgtcggcta
     1921 agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc cagcttgttt
     1981 tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt tccttgcggt
     2041 gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt tccagcagaa
     2101 tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag gctgatacct
     2161 aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct catcaaaata
     2221 tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc taaagcaaaa
     2281 acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga acggatacca
     2341 ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa atccatttca
     2401 cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa aacatgttct
     2461 gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc taatactgct
     2521 tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc ataacttaaa
     2581 cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc attgaaattc
     2641 aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt aattatttgg
     2701 tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt atcatcaaat
     2761 aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt tgcaattcgt
     2821 atataaccac caccaccacg tttgctttct actagataac cacgttggat ggtaaaacgg
     2881 gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc catttcaatc
     2941 cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga ttcaattaaa
     3001 tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg accttaatta
     3061 taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat cctttagaat
     3121 caacaaaaaa aaatacggaa tatactattc cgtatcgacg cgcccaagag gagtcgaacc
     3181 cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc gcatattcaa
     3241 tttaaaaaaa ttataaatca tttgaaaaca aaaagcaaga taaaaaaaat cgggaagaca
     3301 ggattcgaac ctgcgacccc ttggtcccaa accaagtgct ctaccaagct gagctacttc
     3361 ccgtttaaaa aataaaaaag actacgcgcc caagaggagt cgaaccccta accttttgat
     3421 ccgtagtcaa acactctatc cagttgagct atgggcgcat atttgaaatg ccgaggaccg
     3481 gaatcgaacc ggtacggtga tcactcaccg caggatttta agtcctgtgc gtctgccagt
     3541 tccgccaccc cggcgcgatt gctttggcaa agcggaaaac ggggttcgaa cccgcgaccc
     3601 ccaccttggc aaggtggtgc tctaccactg agctatttcc gcgtgtttaa agatgccggc
     3661 taaaggactt gaaccctcga ccctctgatt acaaatcaga tgctctacca actgagctaa
     3721 gccggcatgt aaaacttaaa ttatgcgggt gaagggactt gaacccccac gccgtaaggc
     3781 gctagatcct aaatctagtg cgtctgccaa ttccgccaca cccgcaaaaa tatgagccgt
     3841 acagggctcg aacctgtgac cctctgatta aaagtcagat gctctaccaa ctgagctaac
     3901 ggctcatatg gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc
     3961 tcccagctga gctaaacctc catggataac tt
//
LOCUS       DACELF010000139         3910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.147, whole genome shotgun sequence.
ACCESSION   DACELF010000139 DACELF010000000
VERSION     DACELF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.147"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..933)
                     /locus_tag="IW687_002600"
     CDS             complement(<1..933)
                     /locus_tag="IW687_002600"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0951821.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFL"
     gene            complement(1045..1377)
                     /locus_tag="IW687_002601"
     CDS             complement(1045..1377)
                     /locus_tag="IW687_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0951822.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1525..2400
                     /locus_tag="IW687_002602"
     CDS             1525..2400
                     /locus_tag="IW687_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0951823.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2482..3063)
                     /locus_tag="IW687_002603"
     CDS             complement(2482..3063)
                     /locus_tag="IW687_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0951824.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3069..>3910)
                     /locus_tag="IW687_002604"
     CDS             complement(3069..>3910)
                     /locus_tag="IW687_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0951825.1"
                     /translation="LAELYFSVDRFKEAAVVYETLLASGINEISGISMQERLGQSLSM
                     QGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQLKENQQAINYLEELREVNPHYQSLY
                     LYLGQALQEEEMIEEAQTVLEEGIKENPYQVELYHLASENAFRLHDRQRAEELLLKAL
                     EVGDKQDETLLTLSNLYLDDERYEDVIQVINQMEETANPYAEWNLAHAYNELEDFTVA
                     AVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRAQELLTYYLTLEPGDMEALSLLDDL
                     TER"
BASE COUNT         1085 a          820 c          677 g         1328 t
ORIGIN      
        1 taagaagtac aattcgaatg gttccaaagc tccagtttct cgttgtcgta gtccttggac
       61 aagtgcttga acgtctttaa tcatttggtt agaacacttc cacgatttca aaaaggaacg
      121 aatctcattt tcttttagat ccagttggct gatcaataat gcccatgctt ggctctcttc
      181 tttcaatggg acggatggta attcagcaaa acgtaaaagt gcttcgcctt cttctttcaa
      241 accgggacag caaatgtagc actctgtttc aataaatgca tggattcccg cggcccgttc
      301 atttcccaag agcatcttga tgaattccac atttatacgt tctacagata ttttacctaa
      361 tagctcgtgg ttttctttga tggcgtagaa ggtttcttcc tctaatttga accctagttg
      421 actgacaaaa cgcaacccgc gcatcattcg taaggcatct tcatggaacc gctcatgagg
      481 attgcctaca gctcgcaaga tctgttcttc tagatcttgc agaccatcga acaaatcgat
      541 gatttctcca tcttctttca aagcaaacgc attgatcgta aaatctcttc ttttcaaatc
      601 ttctgccaac gaacgaacga attcgacatg atctggtcta cggtagtcct gataagttga
      661 ttccgtccga aatgtggtga tctcatattg ttcatcattt tctaatacca acacagttcc
      721 atgttcgatc ccaatatcta tcgtttttgg aaaaagctgt ttgatttctt ctggaaatgc
      781 gcttgttgcg atatccacat catggattgg ctggttcagc aagatatcac gaacacttcc
      841 acctacaaaa taagcttcat atcctgcatt ctctaatttt ttcagaactg gaattgcctt
      901 ttggtattcc agaggtaatt gttttaattt catttttcca acctttcctg cgtgtttctt
      961 gtcctgagat aaagactcag ttgcatggtg actgtttgac aagtccgttt cagagtcttc
     1021 aaggccagga ccacttcatc ttgtttactc gtccttacgc tcaaagcgat tatggtctct
     1081 ttgattgaat ttttccatgt ttttttcaaa aacttcagtt aagtcgatat tcaatgaatt
     1141 agccatgatc atcgtaacga ataaaacatc accaagttct tctgctaccg agttaactgg
     1201 ttcagaagcc tttttgcttt tttctccata agtgtggttg acttctcttg ccaattcacc
     1261 tacttcttca gttaatcgag ccatctgagc taatggagag aaatacccca ccttgaattg
     1321 ctggatatat tcatccactt cctgctgcat actgtataaa gaacgctttt tatccatctt
     1381 tttccctcca aaactatctt accaaaactt taacgcaaat cctagataag agcaattgag
     1441 aaaatcgaat ttccatgcta catttaggaa ggaatttcta aatggaaatt ctgtgaaaat
     1501 acataattga aagctggaat tcatatggtc aaacgaaaaa cagtaaaaga agttcttttt
     1561 atcattatag gaacaagtat ttatgcattt ggtcttgttt acttgaacat cgctaatcat
     1621 ttagcagaag ggggagtctc agggatcacc ttgatcttaa gagctttatt tggtattgat
     1681 ccagcttata cgactttatt aatcaacatc ccattgattt tactaggagg aaagatcctc
     1741 ggtaaacgtt ccttggccta tactgttcta ggtacacttt ctttgtcatt ctttttatgg
     1801 atctggcaaa gggttccact agaaatcaat ttacagcatg atctactgat cgtttctcta
     1861 cttgcgggtt tagttgcagg tgtaggaagc gggatcgtct accgcatggg tggaacaaca
     1921 ggcggcagtg atattatcgc tcggatttta gaacaaaatt acggcatcac tatggggcgt
     1981 tccctattgg catttgatat catcgtctta cttgcttcat taacctatat cgacttgaaa
     2041 cgtatgatgt atacgttgat tgtttcgtat gttttcagtc gtgtgattga ttcgatctta
     2101 gatggcggat attctgcaaa aggtctactt gtcgtctcca acaaaagcga agagattgca
     2161 ccactactga tgaccggtct gcaaagaggt gtgactttcc tttctggaga aggcgcattc
     2221 tctggtaatt caaaaaaaat catctatatg gtcgttagct ctagtgaatt aactgaagct
     2281 aagaggatcg tccacgagat tgacgaaaaa gctttccttt caatcatcaa tgtgcatgaa
     2341 gtggaagggc aaggctttac ttatttgaag ccacagacaa gattactgaa aaaactataa
     2401 gaaaaaagac aaagataggc tatgaggaaa gattccacat ccagctgacc tttgtctttt
     2461 ttagtactat actattttta actaattgtt atgttcactt acccggttac ttttcttttg
     2521 tgtctgcaaa aggatctttc ggttaacttc atcagataat ttcaaaaaag catccctatc
     2581 tccatcttct agtgcttgat cgatttgagc ataaagcatt tttagttttg cctcttcttc
     2641 tttttcttga aaatatcgtt caattaattc agtatcttct tcactgactg aagaatccca
     2701 agaagaaaat ggattgtctt ctaagataga tagatattct cttgtctgcc aagcattttc
     2761 gaatatacat tccacatata aaggcttttt ccaattcaaa cggatttcat gaaaaatctg
     2821 gtctgtgtcc gtaaattctt ttccctgcaa aaacaatttc atgggttccc cttcaacaga
     2881 tagatcactg atctgcagtc ctcgatccgc aaaattcgcc ccttcaacaa agtggacatt
     2941 gcttaaaatc gcttcatgat tggcaagata attcaagatc caaaaaactt ctcttcggct
     3001 aaaagataca tgattgacca tccacgttaa aaaattcttc ttttcccgta catcaattag
     3061 catttttctc acctctctgt taggtcatct aataatgaca gtgcctccat atctcctggt
     3121 tcaagtgtta aataataagt caataattct tgcgctcttt tcagctgccc ttcttctctt
     3181 aagaaaaagg catactcttt caagaaatct ggttcatgtg atagttcatg ataagcctgt
     3241 tcatagtgta cagctgcaac ggtaaagtct tctaattcat tataagcatg ggctaggttc
     3301 cattctgcat aaggattagc agtttcttcc atctgattga tcacttgtat cacatcttca
     3361 tatctttcat catccaagta aagattactt aaagtaagta atgtttcatc ttgtttgtct
     3421 cctacttcta aagctttcag taaaagttct tctgctcgct gtctatcatg cagacggaaa
     3481 gcattttccg aggccaggtg ataaagttcg acttggtaag gattttcttt gattccttct
     3541 tctaacactg tttgtgcttc ttcaatcatt tcttcttctt gaagagcttg tcctagatat
     3601 agatataacg attgataatg cggattaact tctcgaagtt cttccagata gttgattgcc
     3661 tgctgatttt ctttcaattg aagattcgta aaagctagct ggaacagtcg atcatctgtc
     3721 ctttcttctt tcagtgcttc ttctaaaggt acgatagctg cttcgaattc tccttgcata
     3781 cttaaggatt gtcctaaacg ttcctgcatc gagattcctg aaatttcatt tatcccgctt
     3841 gccaacaatg tttcataaac cactgctgcc tctttgaaac gatcaactga aaaatacaat
     3901 tcagctagcg
//
LOCUS       DACELF010000140         3883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.148, whole genome shotgun sequence.
ACCESSION   DACELF010000140 DACELF010000000
VERSION     DACELF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.148"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW687_002605"
     CDS             82..3123
                     /locus_tag="IW687_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0951826.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IW687_002606"
     CDS             3313..>3883
                     /locus_tag="IW687_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ0951827.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACELF010000141         3863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.149, whole genome shotgun sequence.
ACCESSION   DACELF010000141 DACELF010000000
VERSION     DACELF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.149"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..348)
                     /locus_tag="IW687_002607"
     CDS             complement(<1..348)
                     /locus_tag="IW687_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0951828.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLID"
     gene            complement(945..3650)
                     /locus_tag="IW687_002608"
     CDS             complement(945..3650)
                     /locus_tag="IW687_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0951829.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         1068 a          778 c          697 g         1320 t
ORIGIN      
        1 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
       61 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
      121 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
      181 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
      241 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
      301 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
      361 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
      421 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
      481 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
      541 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
      601 cctttttgat atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct
      661 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
      721 catctgcggt aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt
      781 tcttattact tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag
      841 ctgaccctcg gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt
      901 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg
      961 ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc
     1021 gttcctacga ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca
     1081 tttggccagt aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc
     1141 aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct
     1201 ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat
     1261 aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac
     1321 acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg
     1381 atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct
     1441 cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt
     1501 gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa
     1561 ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag
     1621 tcactttctt ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct
     1681 tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag
     1741 tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca
     1801 tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata
     1861 aaatcagcgg ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct
     1921 gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca
     1981 tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact
     2041 gcattcgtta cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct
     2101 ttgcttagcg ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg
     2161 acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca
     2221 acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct
     2281 gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc
     2341 tgatcaataa ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac
     2401 tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat
     2461 acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt
     2521 acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca
     2581 tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt
     2641 tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt
     2701 acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct
     2761 tggatcgaag caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct
     2821 gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg
     2881 tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat
     2941 tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact
     3001 tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg
     3061 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
     3121 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
     3181 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
     3241 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
     3301 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
     3361 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
     3421 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
     3481 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
     3541 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
     3601 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
     3661 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
     3721 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
     3781 atattgagaa ataattaagg attacagact ttactttatc atgaaataaa agggaaagat
     3841 aattttatgc caaagttttt atc
//
LOCUS       DACELF010000142         3564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.150, whole genome shotgun sequence.
ACCESSION   DACELF010000142 DACELF010000000
VERSION     DACELF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.150"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(561..1646)
                     /locus_tag="IW687_002609"
     CDS             complement(561..1646)
                     /locus_tag="IW687_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0951830.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1966..2373)
                     /locus_tag="IW687_002610"
     CDS             complement(1966..2373)
                     /locus_tag="IW687_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951831.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(2392..3210)
                     /locus_tag="IW687_002611"
     CDS             complement(2392..3210)
                     /locus_tag="IW687_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0951832.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT         1090 a          560 c          494 g         1420 t
ORIGIN      
        1 ccgttacggg taataccgta acactgaaag gtaaacgcat taaggggtat agacttaatg
       61 ccacctgtgg acgtcgacga aggttggtag ccttccgagt ctcacagggc ctttcttttt
      121 tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatag cagacaatta aatttaaaac aactaaattt atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg gtaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaaat acatatgcaa acactagttc
      361 cctgtttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacatt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataataac tatttatttg
      481 taacatttgt aataaaatta aaattgtaaa aaagttctaa tttaaaattg atcattttta
      541 tagtgttcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc aataaaccag
      661 ttttttacat acacaaaaat caaattctgc aaattattta ctttctcagt cttttgtttt
      721 tgataagctt tcagcaacgt catatataat cctttatccg catcaattcc gtaagattca
      781 agttcttcat acacaactac cgtcttctca agttcctcgg ctttatgttt tgctttgtgt
      841 attgtggtga cggtttgttg ggcttcttcg taagtgttag aaaatgtttt tatcaagctt
      901 aatacctttt ctggtacaaa tgtgtttatg ctgtcttctt tcatgatttc aacgaagtta
      961 tctagcaaaa cttgattttg taattggtct gtttcagtgt cttttaagtg tcttttagtg
     1021 tctaaagatt tattaatata ataatcttgt gcgatttccg catttccagt gttatcaagg
     1081 gtttcagctg ttttttctcg acgtgcgatt tccgcatttc cagtgttatc aagggtttta
     1141 gcagtttcct ctcgacgtgc gatttccgca ttccgagaag aggttgattt atcaatagtt
     1201 tcgcttaaat cgtcatcgaa agaattttga ataaaaagat catcggcact tatttcaggt
     1261 tgaagtaaat agtatttatt tttttcattt tttttcgttt ttgggttaaa ttctcctttt
     1321 acgactgaaa tcaaattata tgtttctaac tcgtttaaaa tggtttttac tttgccttgg
     1381 tagcaattta atttgtccat taggtctgct tgtgtaaatt ccaaatataa tagaccatca
     1441 tcgtcgatcc atttttcatg taaggctcta cgaaattcat ttttaaaaag catataggct
     1501 atttttgagc tgtctttcat tttagagtat ttagaattct gaattaaaaa actaggaaat
     1561 ttataatatt tttcaatata aacattcgtt gctttatatc cccaattttt aattatcatt
     1621 ttttgagatt gattttctgt agacattaaa tttctccttc ttcttagaaa cttgtatata
     1681 ttcaggattt tcaacaagtg aactatacca tgtattaagg gtgaataaaa cactttcagt
     1741 atttaaggta taagttatat attaaaaaaa taagtataag ttatacttta aatacaaata
     1801 atgtataact tatactttaa ataataattt tataatgttg atttaatcgc aaatttacac
     1861 gttgtttatt aagttatacc ttaaatattt aatgtataac ttatacttta atattcattt
     1921 atctagtata acttatactt taattgaata ccgcatgaaa aaaaattatt ttggtaattt
     1981 ttcaaactct tcttgtattt cttctcttat gtgttcttct tttgctactc ctagtctatg
     2041 ctggatatat gagtctgcta atgtattaac aattttttcg aaagaaattc tccctttttc
     2101 actttgtaac tccgtcatat agtcttgtaa aattgagagt ttgctattta atgccggaga
     2161 gatcttttta ggaactgata ccttgtttct tttatcagga attgaaactg gtcggcctct
     2221 ttttttcaat gtaatatttc ttgtgttact caatgcacta ttgctaaagt tatattcacc
     2281 gcttattttc acatcttggc gtttgttatt gggtaataat ggctcaaatt caccattctc
     2341 catcatttta ggaatatcaa aatctttagg cattacttat gcaactcctt attatttatt
     2401 taaaaactct tgtctagcat ctagttctgc caaaatattt ataaatactt ggtgtgcttt
     2461 ataatcccat tgatcaaaat ttccattttt gtattttctt aaatgtatac caattttaga
     2521 ataccttctc aatctttttt gataatgaat gagatttttg gataccacgg ttccgtattt
     2581 ttcaattgct tcttctagga tttcttcatc aaaatcatca tttggttcta ccatacaagg
     2641 tacaatatct ataatttgca aatcaagatc gtatctatcg accatgaatt tttgataatt
     2701 tacatatttg ttgactccct ctaaggattc ttcagctgtt tgaaatgaaa ttatcgaata
     2761 gtctgaagct gccatagcat tatcggaaaa ttcgctaatg gttggtggta catcaataaa
     2821 tatttcttga taattgttct ttattggaag aagtaatttt ttcaagacag taacttggtc
     2881 taattcattt tcatgtttat tagcaaaagc ggagaatgat cgaaaactag tgtcacatgc
     2941 cagaaggtca agattttcat ttaccttaat aatactactt gctaattctc catttgcgac
     3001 accatttgtt atagtggttt tgatatcggt tataccgaaa gttcttgcta tgacttgtgt
     3061 taagttcgct tgagggtcta aatcaataat aagcgttttt ctatttctta tcattgcatt
     3121 gtcataagca agcatgctga ctatcttagt tttacctact cctcctttaa aattgccaac
     3181 tgtaaatgta gtcccttgaa aagtatccat gtaaatcctc ccaatcttta attgataatt
     3241 aaagtataac ttatatatcg gaaatggtca acattaaaag tataacttaa ataataatat
     3301 ctaaaaacgt ttggatatag cggtttttaa taataaaagt ataagttata ccatatatat
     3361 ttatggtata acttatactt ttattattaa aggtataact tataccttaa atattgcttt
     3421 tcgaaaatag ctattctagt ctatctgttt ctttactttt cgctgctgat ttgaattgaa
     3481 ttgatcatat tcattttcta gtattaataa cttttcgtat acatcggtca tattcatact
     3541 agaagcaaga cttagatcat atac
//
LOCUS       DACELF010000143         3543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.152, whole genome shotgun sequence.
ACCESSION   DACELF010000143 DACELF010000000
VERSION     DACELF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.152"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(560..1600)
                     /locus_tag="IW687_002612"
     CDS             complement(560..1600)
                     /locus_tag="IW687_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0951833.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IW687_002613"
     CDS             complement(1949..2275)
                     /locus_tag="IW687_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951834.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IW687_002614"
     CDS             complement(2262..3050)
                     /locus_tag="IW687_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0951835.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1084 a          584 c          487 g         1388 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aac
//
LOCUS       DACELF010000144         3486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.153, whole genome shotgun sequence.
ACCESSION   DACELF010000144 DACELF010000000
VERSION     DACELF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.153"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW687_002615"
     CDS             complement(543..2048)
                     /locus_tag="IW687_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0951836.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW687_002616"
     CDS             complement(2045..2731)
                     /locus_tag="IW687_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0951837.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..>3486)
                     /locus_tag="IW687_002617"
     CDS             complement(2924..>3486)
                     /locus_tag="IW687_002617"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0951838.1"
                     /translation="ARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFS
                     KIMSYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIEN
                     LLLDDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQ
                     RDYFGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT          998 a          700 c          562 g         1226 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaa
//
LOCUS       DACELF010000145         3408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.154, whole genome shotgun sequence.
ACCESSION   DACELF010000145 DACELF010000000
VERSION     DACELF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.154"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(573..1130)
                     /locus_tag="IW687_002618"
     CDS             complement(573..1130)
                     /locus_tag="IW687_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951839.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IW687_002619"
     CDS             complement(1309..1920)
                     /locus_tag="IW687_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951840.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IW687_002620"
     CDS             complement(2033..2290)
                     /locus_tag="IW687_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951841.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IW687_002621"
     CDS             complement(2887..3165)
                     /locus_tag="IW687_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951842.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..>3408)
                     /locus_tag="IW687_002622"
     CDS             complement(3199..>3408)
                     /locus_tag="IW687_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951843.1"
                     /translation="SMFGVVIFGVCAGLFLFILLQTVTLFSSNPTYGWRDSIALGMFA
                     GGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1021 a          707 c          456 g         1224 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattga
//
LOCUS       DACELF010000146         3392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.155, whole genome shotgun sequence.
ACCESSION   DACELF010000146 DACELF010000000
VERSION     DACELF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.155"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..476
                     /locus_tag="IW687_002623"
     CDS             210..476
                     /locus_tag="IW687_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0951844.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IW687_002624"
     CDS             503..802
                     /locus_tag="IW687_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0951845.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IW687_002625"
     CDS             976..1527
                     /locus_tag="IW687_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0951846.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="IW687_002626"
     CDS             1673..1966
                     /locus_tag="IW687_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951847.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="IW687_002627"
     CDS             1959..2255
                     /locus_tag="IW687_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0951848.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..3328
                     /locus_tag="IW687_002628"
     CDS             2330..3328
                     /locus_tag="IW687_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0951849.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1278 a          461 c          667 g          986 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaaga atttctcaac gtattggaat
     3301 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aaaaactgat
     3361 tttgagaaaa attatttaat tttcttagaa tc
//
LOCUS       DACELF010000147         3388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.156, whole genome shotgun sequence.
ACCESSION   DACELF010000147 DACELF010000000
VERSION     DACELF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.156"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            268..594
                     /locus_tag="IW687_002629"
     CDS             268..594
                     /locus_tag="IW687_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951850.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(711..1055)
                     /locus_tag="IW687_002630"
     CDS             complement(711..1055)
                     /locus_tag="IW687_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951851.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1131..2378)
                     /locus_tag="IW687_002631"
     CDS             complement(1131..2378)
                     /locus_tag="IW687_002631"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0951852.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2375..3181)
                     /gene="proB"
                     /locus_tag="IW687_002632"
     CDS             complement(2375..3181)
                     /gene="proB"
                     /locus_tag="IW687_002632"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0951853.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          991 a          669 c          673 g         1055 t
ORIGIN      
        1 caagcttttg aacgccgttt accaagaggt tgtgagactg tcgtttaact ctgaatcaca
       61 aggaacaatt tcaaaaaaca gtttctcatg tttgttgaat attgtgactt atgattgagg
      121 agttggcaga agaacaccat ttattcggaa aaaggggatg tgacacgatt tttgtcacat
      181 tccctttttt tctcaaaata atgaaactgg aaaattttca gtaggtaagc taaaattcat
      241 ttaagaaaaa gaatgggggg ataatagatg ctggaagaat attttcagga agtcgggttg
      301 acactcgttg aattacccat tgaattttca gatgatcggt tgatttatcg tggctatatc
      361 aaagaattgc caataatcaa tgcggaaggt gaaactagaa aagcgatgca gcaaaaaata
      421 gctggtatgt atcaggtgta tcgggaacag ctactggctg aatcagcgga agaagaaaaa
      481 gaaaaaacga tgaacttgtc catggatgag ctgcttcgtt attatgatgg cgaaacattt
      541 gacggatttt cgcttcttaa cgaagaggaa acagatttac caggcagctg gtaggcgaac
      601 gccgtttata tggaaaaaga ggctgtgaca aaatctttgt cacagcctct aaaaagtccg
      661 gcagtttttt gccgatcttc aaggcactct acttatcgta cggatatttt ttagcggaaa
      721 gtccgattca agtggttttt attcagctga tatttataag ataagtgaaa aatcccagca
      781 atcagtagcg taatcgtata agcgattgaa tgatcgaaaa aagaatagta attgattgtc
      841 gctaataagc ataatgcaac catgtaacta atgtgcaagc gtgtaaccag ccctttttgc
      901 ccgattttgt caaaatactg cgaccatttt tcccgttgac tccctatgct tcgttttttg
      961 aattgaaaga aaagggataa aataccgact cccagaaaag cagcgatatt cagagaaata
     1021 aggtaaataa aaaaaatcat taaaatttca aacatattga cacctttcta gttggataga
     1081 acctttacag aaaaaaggca aaaggcggga ataatgtttc acacgaaaca ttattcgcgg
     1141 atttgtccat cgccataaat gatatatttt gtggaagtta attcatttaa ccccattgga
     1201 ccacgggcat gcaacttttg agtagagata ccaatctctg caccaaaacc aaataccgat
     1261 ccgtctgtaa aacgagtgga ggcattgaca tagacagccg ccgcgtccac ttcatttaag
     1321 aaacgttgac tagccgcata actatctgtc acgatcgttt ctgaatgttt agtattatgc
     1381 cgattgatat gtgcaattgc ttcatccaaa gaatcgacca ctttgacagc aaggatataa
     1441 tctagatatt cagtatccca atctgtgtct tctgctaaag cagccgtctc tagatattct
     1501 aacgcacgag catccgcacg taaagagacc ccgtgttcta ccagttcttt ttcaatagct
     1561 ggcaagaaaa aaggtgcgat ctcagaatgg atcagcaatg tttctgccgc gttacagaca
     1621 gatggacggg aagcttttgc attgacgatg atgttcactg ccatagataa ctgtgcttct
     1681 ttatcgatat atacgtgatt atttcctgta cctgtttcaa tgataggtac cgttgcctgt
     1741 tctttgactc gttggattaa ttttgctccg cctcgaggaa tcagcacatc taagtattct
     1801 gtcaatcgca tcaactgtcc tgcagtttca tgagaagtat catcgacaaa ctggatggca
     1861 taaggagatt cgccagcact gaccagtact tgctgcaata attggactaa taattgattc
     1921 gagaagaatg cttctttgcc accgcgaagg ataacggcat tccctgattt gaatgctaga
     1981 ctagctgcat ctgtcgtgac atttggacga gattcgtaaa tgatgccggt cacgcctagt
     2041 ggcacgcgct ttttgccgat tctcaatccg tcggtgttcg tccacatctc atctactttt
     2101 ccaataggat cgtctaacga agcaattttt tcgatctctg tagccatggc attgattcgc
     2161 tcaggtgtca gctgcaagcg gtcttgcatc gtttcactga tattgttttc gactgctttt
     2221 tctaagtctt tatgatttgc ttcaatgatc gcttgttgat tctttttgat ggtttgtgcc
     2281 atttgaacaa gtagctcatt tttgcggttt gtaggcataa gtgctagttg ttgcgcgcta
     2341 tcttttgcct gttgtcctaa ttgcatcaag tcagtcattt caagtcctcc ttaaataaag
     2401 tacctacttc ttttcctgca agaatatcaa aaataattgc cggatttttt ccatttgcta
     2461 aaatcattgc gctgtttgct tcaaatatcc tagaagcggc ttttaattta ctctgcatac
     2521 cgcctgttcc aaatgtgcta ccagcaccac ctgcttgtgc cagaatcttt tcatcgattt
     2581 tatcgatata gcgatacatc tgagcctcgg gatttgtagt cggattatct gaataaaacc
     2641 cgtcgatatc agaaaggacg atcaaaaggt ctgccgcgac aagttttgcc atgatggctg
     2701 acagctgatc attatcgcca aattttgtca gatgatctaa ttcatccact gctacagtat
     2761 cgttttcgtt cacgataggg atcgtgtcca taatcaaaag ctgttccaat gtattcctca
     2821 catttttacg actttcagga taatctacca cgtctcttgt cacgaggatc tgtgccgtct
     2881 gttgattata tacactgaat ctttgattat agatattcat cagttcggct tgaccaacag
     2941 ccgccgcagc ttgctgttca ggaatcaaag tcggacgttc tgacaaatgt aatttatgca
     3001 taccaacgcc tacagctcct gatgagacta agatcacttc ctttccccga tttctaagat
     3061 cagaaaggac aaaagctaac tgatcgattg ccgcaagatt gatatttcca ttgggataaa
     3121 tcaatgtact tgttcctact ttgacaataa ttcgttttgc atttttaatt tctttacgca
     3181 tgtgacacct caatcaaata tccactcatt aatttcgttc tattctactt gttttgacga
     3241 gtaaagtcat gtaaaaaata gtttttttgt acaacttgca gtcgtttgga aaaagaacga
     3301 attaatggtt tactttaaga tagcagaatt tcttgaagaa tttgtaaaga aagtctttta
     3361 cagcctcgga tatgttagaa taggagta
//
LOCUS       DACELF010000148         3341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.157, whole genome shotgun sequence.
ACCESSION   DACELF010000148 DACELF010000000
VERSION     DACELF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.157"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..238
                     /locus_tag="IW687_002633"
     CDS             <1..238
                     /locus_tag="IW687_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951854.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IW687_002634"
     CDS             252..440
                     /locus_tag="IW687_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951855.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IW687_002635"
     CDS             452..1063
                     /locus_tag="IW687_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0951856.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IW687_002636"
     CDS             1048..2439
                     /locus_tag="IW687_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0951857.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IW687_002637"
     CDS             2655..2957
                     /locus_tag="IW687_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951858.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..3297
                     /locus_tag="IW687_002638"
     CDS             2950..3297
                     /locus_tag="IW687_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951859.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
BASE COUNT         1306 a          469 c          652 g          914 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa aggctcttcg tcaaatcgtg ttggtgaaga
     3301 caaatttgaa taaaacttag tccgatatgc ggcgcgttct g
//
LOCUS       DACELF010000149         3295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.158, whole genome shotgun sequence.
ACCESSION   DACELF010000149 DACELF010000000
VERSION     DACELF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.158"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..1103
                     /locus_tag="IW687_002639"
     CDS             135..1103
                     /locus_tag="IW687_002639"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0951860.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="IW687_002640"
     CDS             complement(1420..1944)
                     /locus_tag="IW687_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0951861.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..>3295
                     /gene="scm"
                     /locus_tag="IW687_002641"
     CDS             2231..>3295
                     /gene="scm"
                     /locus_tag="IW687_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0951862.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1049 a          667 c          611 g          968 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DACELF010000150         3292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.159, whole genome shotgun sequence.
ACCESSION   DACELF010000150 DACELF010000000
VERSION     DACELF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.159"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW687_002642"
     CDS             37..588
                     /locus_tag="IW687_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ0951863.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IW687_002643"
     CDS             1264..1530
                     /locus_tag="IW687_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951864.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IW687_002644"
     CDS             1588..2100
                     /locus_tag="IW687_002644"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0951865.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IW687_002645"
     CDS             2097..3134
                     /locus_tag="IW687_002645"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0951866.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACELF010000151         3231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.160, whole genome shotgun sequence.
ACCESSION   DACELF010000151 DACELF010000000
VERSION     DACELF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.160"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            183..1137
                     /locus_tag="IW687_002646"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IW687_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IW687_002647"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IW687_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IW687_002648"
     CDS             2184..2663
                     /locus_tag="IW687_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0951867.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3231
                     /locus_tag="IW687_002649"
     CDS             2679..>3231
                     /locus_tag="IW687_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0951868.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          979 a          539 c          751 g          962 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc g
//
LOCUS       DACELF010000152         3220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.161, whole genome shotgun sequence.
ACCESSION   DACELF010000152 DACELF010000000
VERSION     DACELF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.161"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW687_002650"
     CDS             50..1438
                     /locus_tag="IW687_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0951869.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3220
                     /locus_tag="IW687_002651"
     CDS             1776..>3220
                     /locus_tag="IW687_002651"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0951870.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga
//
LOCUS       DACELF010000153         3103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.162, whole genome shotgun sequence.
ACCESSION   DACELF010000153 DACELF010000000
VERSION     DACELF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.162"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1072
                     /locus_tag="IW687_002652"
     CDS             <1..1072
                     /locus_tag="IW687_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0951871.1"
                     /translation="VAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIV
                     SDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIR
                     KNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQER
                     NSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKA
                     AVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFV
                     GEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNP
                     MMILTMPVAQPVKPGDMIRKRK"
     gene            1206..2552
                     /gene="gor"
                     /locus_tag="IW687_002653"
     CDS             1206..2552
                     /gene="gor"
                     /locus_tag="IW687_002653"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0951872.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1039 a          493 c          685 g          886 t
ORIGIN      
        1 agttgctttt gcaaaagaac atgatgcgaa ggtatacgtt gctgctaata tggtcaccca
       61 tgaaggaaat gaagatggag caggggaatt tttccgcagt ttgcgtgatg tcggtatttc
      121 tgcagtgatt gtttctgatc cagctttgat cgaaatttgt gcgacagaag ccccaggtct
      181 gccgatccat ttatccactc aagcttcagc aactaatttt gagacattgg aattctggaa
      241 agaagaaggg ttggaacgtg tcgttttagc tcgtgaagtt tcaatggagg aagtggcaga
      301 gattcgtaaa aatacggata ccgaaatcga agcatttatc catggtgcca tgtgtatctc
      361 ttattctggt cgatgcacat tgtccaacca tatgtcgatg cgggatgcaa accgtggcgg
      421 atgttctcaa tcttgtcgct ggaagtatgg attgttcgat atgccttttg gtcaagaacg
      481 aaacagttta actgatacag gcgaagtgga agaagaattc tcgatgagtg cagtggatat
      541 gtcaatgatc gaacatattc cagaattgat tgaaaacggc gtagacagct tcaagatcga
      601 aggaagaatg aaatcgatcc attatgtatc tacggtgtca aatgtctata aagcggctgt
      661 agacagttat atggaagatc ctgaaaacta tgtctgcaag caagaatgga tcgacgagtt
      721 atggaaagta gcacaacgag aattagctac aggtttttac tacaatacac caactgaaaa
      781 cgaacaatta ttcggtgaac gtcgtaaaat cccagtatat aaatttgtcg gtgaagtatt
      841 atcttatgat gaggcaacac agattgctac gatacgtcag agaaacttgt tttctgtagg
      901 agatgaaatc gaattttatg gtccaggatt cagtcatttc agtcaaaccg ttgaagttat
      961 gcataatgaa gaaggcgaat ccattgaccg agcaccaaat ccaatgatga ttttaactat
     1021 gccagtcgca cagccggtga aacctggaga tatgatccga aaacgtaaat aaggaaaaag
     1081 ctgatatatc cctgttttaa gcatcttcgc atacaaaatt caagaaaagt gttacacttg
     1141 ttttctgtag aaaaaggatc gttaaataaa ggttttgatt gatctcttca ataaggagga
     1201 acaaaatgca aatatatgat tacatcgtaa ttggtggcgg ctctggcggt attgcttcag
     1261 caaacagagc agggatgcat ggcgcaaaag ttttgctgat tgaagcgaat gaattgggcg
     1321 gaacatgtgt caacgtcggt tgtgtaccta aaaaagttat gtggcaagca agtacgattt
     1381 tggaaacaat tgaacgagat gcctcaagtt acggtatcca agcagattta aaaaatgttg
     1441 atttcgccgg tctagtagaa agacgagaaa aatatattga ttttctacac ggtgcttatc
     1501 aaagaggatt agatagcaat catgtcgagg ctataaaagg ttacgctact tttatcgata
     1561 gtcaaacgat cgaagtaaac ggtgaaacct atcgtgctcc taaaatcttg atcgctactg
     1621 gaggccgtgc aagtaaaatg accattcctg gtggagaata cgcaattgat tcaaatggat
     1681 tttttgaatt aacagagtta cctaagagag caatcgtttt aggtgctgga tacattgcgg
     1741 ctgaactttc aggtgtattt cgtggtttag gtagtgaagt catgtgggca tatcgaaaag
     1801 aacgaccact tagaacgttt gataaaatgc tttcggacaa tttgatccaa atgtaccaag
     1861 aagctggaat aaaaacttac gcacatcata ttgctaaaga aattactaaa aacaatgacg
     1921 aatatacggt gctatttgaa aatggtgaaa ccttaactgg tgattgtgta ctttttgccg
     1981 gtggacgtgt accgaatacc gaaaaattag gtctggaaaa tacaaatgtg gaattggaca
     2041 aaaaagggtt tatcaaagta gataaatacc aaaatacaac ggatgaacat atttttgctg
     2101 ttggtgatgt gatcggtaaa ttagatctga ctcctgttgc aatcgcagct ggtagacgtt
     2161 tatcagaacg tttatttaat ggaaagaaag attcttatct tgactataaa cttgttccga
     2221 cagtcgtctt tacgcatcca ccaattgcaa caattggttt gacagaagaa gaagcattgg
     2281 aaaaatatgg cgaaaatgaa ttgaaagttt atcgttcacg ctttacccca atgtactttg
     2341 cattaaatga ttatcgccaa aaatgcgaaa tgaaattgat ctgtgtcgga aaagaagaaa
     2401 gaatcgtcgg cttacatgct attggtgttg gtgtcgatga gatgctgcaa ggttttgccg
     2461 tggctatcaa gatgggtgca actaaagaag actttgacaa tacagtagcc atccatccaa
     2521 caggagcgga agaattcgta acgatgcgat aatttaatat taacagttcc aacagctttt
     2581 tattgaacaa accatgtaaa accagaaaat cagtatactg attttctggt tttttgtttt
     2641 ttagttattt atgtaagatt gcttcgataa ttctcaaaac gaaataagta gggaagttgg
     2701 agattttgat tatcttcgta actgacgaag agtaaatgaa tccatttggt atgcactctt
     2761 aagttcaaaa tattggtatc gatgtttaaa agaaatagaa atgtatcgaa gaacagatgt
     2821 ttaaaaaagt gtgactttgt ataaataaat tagaagaagc aaacgaggat gttaaaagaa
     2881 taccatatag ttaccaaacc aagaaagttc tcgtcaagtt cgctggtacg aatcaaatca
     2941 agtgaaaaaa gggactgtga aaaaaattgt gtcatagtta gtcacttttt ccgaacaaat
     3001 agtgttcaat cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     3061 ttttccattt gtttctctaa tactcaggac aaaacactga ttt
//
LOCUS       DACELF010000154         3027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.163, whole genome shotgun sequence.
ACCESSION   DACELF010000154 DACELF010000000
VERSION     DACELF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.163"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            343..441
                     /locus_tag="IW687_002654"
     CDS             343..441
                     /locus_tag="IW687_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0951873.1"
                     /translation="MLEKLITLVIAPLFVGLVIELISHWLDEKDDN"
     gene            1167..1451
                     /locus_tag="IW687_002655"
     CDS             1167..1451
                     /locus_tag="IW687_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951874.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEILIGGMI"
     gene            1454..1870
                     /locus_tag="IW687_002656"
     CDS             1454..1870
                     /locus_tag="IW687_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951875.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNQPKNHQKGTNKTVTSSNFDPTKFLKR"
     gene            complement(1958..2680)
                     /locus_tag="IW687_002657"
     CDS             complement(1958..2680)
                     /locus_tag="IW687_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HAQ0951876.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMD
                     EKTNKKDWTSSDVIRNGKHYVKPHYHVLYIARNPVTIESVRKKIKRKLGNNSVAHLEI
                     IDHIKGSYEYLTHESKDAKEKNKHVYDKKDILHINDFDIDRYVTLDESQKKELRNQLF
                     DLVNHCGIVNMKELAAFLDWRGQEYGITNQRDVVDVIASNSNLFRMMFDANYQNGFRP
                     QYAKRIDSETGEMIEGVNKHVE"
     gene            complement(2761..2910)
                     /locus_tag="IW687_002658"
     CDS             complement(2761..2910)
                     /locus_tag="IW687_002658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951877.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKK
                     SEKGN"
BASE COUNT          953 a          640 c          454 g          980 t
ORIGIN      
        1 aaaaaaaaag gctctatcca ttggtacagt aaggataaag cactttttgg cacaaaaatt
       61 ggcactcggc acactatggg gggggtcgta gtacggaagc aaaattcgct tcctcgcccc
      121 ccagttgtcc caaattccaa atttttttca aaaattttcc agcgcttccg ctcggcaaaa
      181 tcgcaagcga ttttaacact gcctttttaa gggggtgttc aaattttgaa catttaaccg
      241 cctgttagaa atacggttaa gtttttccca ctcattctca aagaaaaaaa gagatatgct
      301 ataatcacac atggcaactt gccccgaaag ggggtgttct ctttgctaga aaaactcatc
      361 acgttagtta tcgcaccact ttttgtaggg ttagtcatcg aactaatttc tcactggtta
      421 gacgagaaag acgataacta aagcaagttg ctttctaatc cacacgtttg tttccaccac
      481 aagcgcaaaa aaaatcccaa cctacggaca taggttggga ttttgtgttc tcttgctctt
      541 gcaaaaactc atcacgtttc cacactgcta ttataacaag caataactga aaaatcaaag
      601 acacaagacc ttggggaatg tcattcccca aaccccttga gcctcctcct gccgaaatat
      661 ttctacgctc tcgctcctca tttcattcag ttctcggctc cacaatctaa cacgccgttt
      721 cgctaccagt cgtctttcag actccattct cgctcctctc acgtgttttg acgattgcgc
      781 cgataactaa acgaaataaa cgctaaaacg tcttagaacg caaaatatga gcttctaaga
      841 ctattccatt tcagttacat actctttata atcgttccat ttgatttctc tttcgtgcca
      901 ttcattatcg cttttcgcaa acagaacaac acgaatccat gtcactattt ttttgttttt
      961 taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat aggctgtgcc
     1021 tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg tagcttatgt
     1081 atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat acataaatta
     1141 tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt gatccaaagt
     1201 ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga gaaaaatgga
     1261 gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa cttacgactt
     1321 acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa acagaaaata
     1381 ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatt cttatcggag
     1441 gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt accctagtca
     1501 tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact actcggaagc
     1561 cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta ccagcaatca
     1621 ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca tcgcagaatt
     1681 cgctgattta tatctaaaag atacaggcgt tcttatcggc acaaattcta aagtatatca
     1741 agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc aaccaaagaa
     1801 tcatcaaaaa ggaacaaaca aaacagtcac ttctagcaat ttcgacccaa ctaaatttct
     1861 aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttccc aatattctat
     1921 tttttcattc caagcagtta acggaagttt tcgatattta ttcgacgtgt ttattcacgc
     1981 cttcaatcat ttctccagtt tcagaatcta tcctttttgc atattgcggc cgaaatccgt
     2041 tctggtaatt agcatcaaac atcatacgga aaagattgct gtttgaagct atgacatcaa
     2101 caacatctct ttgattagtg attccatact cttgacctct ccagtcaaga aatgcagcta
     2161 actctttcat attgactatt ccacaatgat taacaagatc aaacaattgg tttctcaact
     2221 ctttcttttg actctcgtcc aaagttacat aacgatcaat atcaaaatca ttaatatgca
     2281 agatatcttt tttatcgtat acatgtttat tcttttcttt tgcatctttt gattcatgcg
     2341 taagatactc atacgagcct ttgatatggt caattatttc taagtgagca accgaattat
     2401 ttcccaattt tctcttgatt ttctttctta cgctctcgat tgttacagga ttgcgagcaa
     2461 tataaaggac atgataatgt ggctttacat aatgcttacc gtttctgata acatcactac
     2521 tagtccaatc ctttttattt gttttttcgt ccatatcgtg taacggacta accgccattg
     2581 atactcctaa gctctcaagc tgttctctcc aattctctgg aatagattcg ggatatagta
     2641 aaaaaccaaa atgtctagct ttcgttatat tcttattcat gatatactta cctcaattga
     2701 acactatcag ttaagcggaa acggtgcgcc aacacatatg tttctgctct ttttttgcgc
     2761 tcaatttcct ttctctgatt ttttcaagtc ttctgttact agcaatttaa catatgtcga
     2821 catactcaaa cttttgtctt ttgctttttc ctcaagtttt cgcttaattt cactcggtaa
     2881 aattactacc agtctttctg attccgccat gcctttcagc ccctttagtt aattgttaat
     2941 atcatggtaa acttttgaca actgtattgt caatattttt ttctattttt caaaacggcg
     3001 gtcaaaacgg cggtcaaatg gcggtcg
//
LOCUS       DACELF010000155         3012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.164, whole genome shotgun sequence.
ACCESSION   DACELF010000155 DACELF010000000
VERSION     DACELF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.164"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW687_002659"
     CDS             complement(106..1053)
                     /locus_tag="IW687_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0951878.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW687_002660"
     CDS             1299..1763
                     /locus_tag="IW687_002660"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0951879.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW687_002661"
     CDS             complement(1825..2868)
                     /locus_tag="IW687_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0951880.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          996 a          597 c          577 g          842 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gc
//
LOCUS       DACELF010000156         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.165, whole genome shotgun sequence.
ACCESSION   DACELF010000156 DACELF010000000
VERSION     DACELF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.165"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..1172
                     /locus_tag="IW687_002662"
     CDS             204..1172
                     /locus_tag="IW687_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0951881.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IW687_002663"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IW687_002663"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0951882.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IW687_002664"
     CDS             2202..2810
                     /locus_tag="IW687_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0951883.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          709 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaag
//
LOCUS       DACELF010000157         2837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.166, whole genome shotgun sequence.
ACCESSION   DACELF010000157 DACELF010000000
VERSION     DACELF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.166"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(123..647)
                     /locus_tag="IW687_002665"
     CDS             complement(123..647)
                     /locus_tag="IW687_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951884.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW687_002666"
     CDS             complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW687_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0951885.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW687_002667"
     CDS             complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW687_002667"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0951886.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2369..>2837)
                     /locus_tag="IW687_002668"
     CDS             complement(2369..>2837)
                     /locus_tag="IW687_002668"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0951887.1"
                     /translation="QAHGLSFSVQPGVKIPPSLRNIYKELYDDLGIAPVQHGNLVSWA
                     KQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIVFILWGKPAQEKIK
                     MIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPIDWQLPETV"
BASE COUNT          747 a          662 c          487 g          941 t
ORIGIN      
        1 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
       61 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
      121 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
      181 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
      241 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
      301 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
      361 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
      421 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
      481 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
      541 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
      601 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
      661 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
      721 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
      781 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
      841 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
      901 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
      961 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     1021 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     1081 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     1141 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     1201 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     1261 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     1321 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     1381 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     1441 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     1501 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     1561 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     1621 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     1681 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     1741 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     1801 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     1861 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     1921 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     1981 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     2041 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     2101 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     2161 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     2221 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     2281 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     2341 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     2401 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     2461 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     2521 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     2581 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     2641 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     2701 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     2761 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     2821 ataagccatg agcttga
//
LOCUS       DACELF010000158         2808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.167, whole genome shotgun sequence.
ACCESSION   DACELF010000158 DACELF010000000
VERSION     DACELF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.167"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1737)
                     /gene="tkt"
                     /locus_tag="IW687_002669"
     CDS             complement(<1..1737)
                     /gene="tkt"
                     /locus_tag="IW687_002669"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0951888.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKD"
     gene            complement(1839..2090)
                     /locus_tag="IW687_002670"
     CDS             complement(1839..2090)
                     /locus_tag="IW687_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0951889.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2237..>2808
                     /gene="lexA"
                     /locus_tag="IW687_002671"
     CDS             2237..>2808
                     /gene="lexA"
                     /locus_tag="IW687_002671"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0951890.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILD"
BASE COUNT          743 a          632 c          533 g          900 t
ORIGIN      
        1 atctttgcct ttttcagcca attctttttg agctttcaca gccaaatcaa cttcagaacc
       61 tgttgcaatc aaaatacctt ctggtgtttc tccttgtgat ggagaaagaa catacgcgcc
      121 ttttttcacc atttcatctg ctacttcttt tgtagaaggc aatactggaa ggttttgacg
      181 actcaataca aggatagtag gcgcatctgt tgtctccatc gctactttcc aagcagcacg
      241 tgtttcattt ccgtctgccg gacgaatcac ttgaacacca ggcatgcagc gaacagatgc
      301 tagttgttca atcggttcgt gcgttggtcc gtcttctccg acagcaaccg agtcatgtgt
      361 taagacgtaa acaacaggag tatgttggat tgctgctaaa cgaactgctg gtcttagata
      421 atcgacaaag acaaagaatg ttccaccgta gatacgagtt ccgccatgaa gttggatacc
      481 attcatagcc gaagccattg caaattcgcg gacaccgaac cagatgttgc gtccttcata
      541 atgttcagga gtgaaatctt tatctgcagc caccattgta ttattggaac ctgacaagtc
      601 cgctgaacca ccccagaagc ttggtatagc tttcgataat tcttggatga cttctttact
      661 tgaaacacgg cttgcttgac tgcttccaac ttcataagtt ggcaattctg catcccagtt
      721 ttctggtagt ttgccatcaa aagcatcttc aaattgttgt gctaattcag gataagcttt
      781 tttataattg gcaaacattt cattccatgc atcttcagct ttttgtcctt cttctatcat
      841 cgtttgatgg aagcgggcag ctacttcttc tggaacagtg aaatctggat actcccaacc
      901 ataaacttct ttagccatct tgatgccatc ttcaccaaga ggagcaccat gaacagcaga
      961 tgttccttct ttaggagcac catagccgat gactgttttt acttcgatca atgttggttt
     1021 gtctgtttca gcttttgctt cttcaatcgc tttagagatt gcttctaggt catttccgtc
     1081 tttcactaag atatgttgcc agccatatgc ttcatagcga gcaccaacgt tttctgtgaa
     1141 agcttttgaa gttggaccgt ctagtgaaat atcgtttgaa tcgtataaaa cgatcaattt
     1201 ccctagtttc atatgacctg ccatcgaact tgcttcttga gatacccctt ccatcaagtc
     1261 tccgtcaccg catattgcat acgtatagtg atccatcaca ttaaagtttt ctttgttata
     1321 cgttgctgca aggtgcgctt ctgccattgc cataccgaca gccattgcaa tcccttgtcc
     1381 taatggccct gtagtggctt ctacaccatc tgtatgatgt acttcaggat gtcctggtgt
     1441 tttagaatcc cattgacgga attgtttcaa atcatcgatt gtcacttgat agccagctaa
     1501 atgaagcaag ctatacaaca ttgctgaccc atgtcctgcc gaaagaacga aacggtcgcg
     1561 gtcagcccag ttttttgatg tcgttggatt tactttcagg tgttttgtcc ataacgcata
     1621 tgccataggt gctgcgccca tcggcaatcc aggatgtcct gagtttgctt tttggactgc
     1681 ttcaatactt aatgtacgaa ttgtgttaac gccaagttgg tcaattttgt caaacaaaat
     1741 aacccctcct attaatttag gtttttttta cctttatttc ttacactttc attctataag
     1801 atgtcacacg aaaaagcaat ctttttcagt attttttact aaaagttgtc atttcggttg
     1861 tgtaagcctt ttttcttttg gatttctttt aatttttccg gggtcacgtc attgccctca
     1921 ggatcgacga ctttcattcc ttcgatatga tgacgcatac cggaacgaaa agcttccaaa
     1981 tactctttgc gcaagttgat ttgttcttgt tcttcggctt ttgagagacc attttccttc
     2041 tttttacgtg caagttcatt gatccgctct attttttcag gtgacagcat gctcagccct
     2101 ccttttactc tccttcatat tatataaaaa ctgaaaaaaa aacaactaga taaactaaat
     2161 aaaatacgaa cattcgtttg atttagcgct ttttatatgg tactatttat ttatcaagtg
     2221 aaagaaggtg taccttgtgg cccgacaaac tgaatcaaga caaatcgaag tcttacgata
     2281 tatccatgaa caagtaaccc aaaaaggata cccgccgact gtccgtgaaa tcggggaagc
     2341 tgttcagctt tcttctactt ctactgttca cggtcatctt gcacggcttg aaaaaaaagg
     2401 gtttatccaa agagatccga cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga
     2461 aaagattggg attcgtccaa caactattcc agtacttggc gtcgtaacag ctggagaacc
     2521 cattttagca gtagaggatg cttcagactt ctttccaatc cctcctaatc tttcttccga
     2581 agaaggcagt ctctttatgc tgacaattcg tggagatagt atgatcaatg ctgggatatt
     2641 ggatggtgac aacgtgatcg ttcggaagca agaaactgct caaaatggcg atattgtcat
     2701 tgcaatgact gccgaggacg aagcgacttg caaacgattc tataaagaaa aaaacttttt
     2761 ccgtctgcag cctgaaaacg acttgctcga accaatcatc ttagatca
//
LOCUS       DACELF010000159         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.168, whole genome shotgun sequence.
ACCESSION   DACELF010000159 DACELF010000000
VERSION     DACELF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.168"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW687_002672"
     CDS             <1..1023
                     /locus_tag="IW687_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0951891.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="IW687_002673"
     CDS             1157..2500
                     /locus_tag="IW687_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0951892.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..>2784
                     /locus_tag="IW687_002674"
     CDS             2612..>2784
                     /locus_tag="IW687_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0951893.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1111 a          361 c          517 g          795 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctgggaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac tttt
//
LOCUS       DACELF010000160         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.169, whole genome shotgun sequence.
ACCESSION   DACELF010000160 DACELF010000000
VERSION     DACELF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.169"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW687_002675"
     CDS             <1..197
                     /locus_tag="IW687_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951894.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW687_002676"
     CDS             314..811
                     /locus_tag="IW687_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0951895.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW687_002677"
     CDS             898..1290
                     /locus_tag="IW687_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0951896.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW687_002678"
     CDS             1274..>2778
                     /locus_tag="IW687_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0951897.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACELF010000161         2701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.171, whole genome shotgun sequence.
ACCESSION   DACELF010000161 DACELF010000000
VERSION     DACELF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.171"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..816
                     /locus_tag="IW687_002679"
                     /pseudo
     CDS             1..816
                     /locus_tag="IW687_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1062..1658
                     /locus_tag="IW687_002680"
     CDS             1062..1658
                     /locus_tag="IW687_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0951898.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1671..2570
                     /locus_tag="IW687_002681"
     CDS             1671..2570
                     /locus_tag="IW687_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0951899.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2573..>2701
                     /gene="ssb"
                     /locus_tag="IW687_002682"
     CDS             2573..>2701
                     /gene="ssb"
                     /locus_tag="IW687_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0951900.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
BASE COUNT         1015 a          443 c          579 g          664 t
ORIGIN      
        1 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
       61 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
      121 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag
      181 gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
      241 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
      301 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
      361 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg
      421 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
      481 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
      541 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
      661 tggcagcttg tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca
      721 acgattgttt taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg
      781 aacttcaaga aaagaacacg ccgcactgca aaataatgga gggttaatct tatgcgtaat
      841 accacacaca aacaacaaga aatcgaaaat caaaaagcca aaattcgttc agaacaggaa
      901 aaaatgcgtg ccagcaaacg aagactgcag caattgaaga accagcagag gattcaaaaa
      961 gaaatcgaat tagatcgata aaatcttcct aactattgta tacgtataaa cttatgttat
     1021 actgttttta taagtttata cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat
     1081 acaagctgct agaagaattt aatggaactt tggctttgaa ggatgccaaa aaagcgggca
     1141 tttctcctgt aacgatcaaa cgtatggtag atcgagggga gctggatcga gagtatccag
     1201 gttttttcac cttacctggt caatttcctg atgaattgtt tatggcgcaa aaaaaatatg
     1261 aacgcggcat tatttcacat attaccgcgt tagatttgta tgatttaaca gatatgatcc
     1321 ctagacagat cgatctgact gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg
     1381 aattcgcagt tgagctacac tatacaaaag cggaatggta tgaattaggt aagattgaaa
     1441 taaaaagtcg ctatgggaat cctattatag cttatgatcc agaaaaaacc ttgtgtgaca
     1501 tttggaatcc ctggtataac gttgaggatg aaatcaaagt aaaagcaatc aaaaactaca
     1561 tggagtcgga cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt ttgcctacag
     1621 ataaaacgat gcgctcttat attatggctt taaattaaag gagaatacac atgacaccta
     1681 cgcaattaaa agcaaaagtg aagaatattt ctagagaaaa agaagttgat ccgcagcttg
     1741 ttttaagaca ttttatgatg gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg
     1801 aaaattttgt gttaaaaggc ggcttcttaa ttggctcaaa gtatggcatt gaaaatcgaa
     1861 caactaaaga cattgatacg actttacggg aaatgaaagt aaccaaagaa acattgacaa
     1921 cggttctaaa cgatattttt tcaacgccga cgaaagaagg cattcaattt gagattcaag
     1981 gaatgaaaga aacgagggaa gctgattatt atccagggtt tagcttacgt gtactggcac
     2041 atttggaaaa catgcggcca gattttaagg tagatgttac tacaggtgac tcgatctatc
     2101 ctgcgacgat cactcacagt cataaattga tgtttgagga tcgaacgatt cctttggaat
     2161 cctatcctac agaacaaatt attgctgaaa aattaagtgc aacctttgat tttttaacag
     2221 ataattcgag aggaaaagat ttttatgatc tctatactat tcctaaaatg gagaagatcg
     2281 atgagaagac cttgaaagat agtgtgagaa atacatttac acgtcgagga aaaactgatc
     2341 cgttaaagat atactatgaa aaggatatgg atgtactgaa aaatagtcac gagctaaaag
     2401 atacctggaa gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga
     2461 caatggattc gattagtaat ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa
     2521 aactagctta tcaaagacac caacagatga atagagaaat ggagagataa agatgattaa
     2581 taatgtgaca ttagtaggac gcttaaccaa agatcctgat ttacgttata ccgcaagtgg
     2641 tactgcagta gcgactttta ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa
     2701 t
//
LOCUS       DACELF010000162         2659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.172, whole genome shotgun sequence.
ACCESSION   DACELF010000162 DACELF010000000
VERSION     DACELF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.172"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            440..>2659
                     /locus_tag="IW687_002683"
     CDS             440..>2659
                     /locus_tag="IW687_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0951901.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     "
BASE COUNT          820 a          487 c          628 g          724 t
ORIGIN      
        1 aaacaaaaat tttgttttcg ttacatcttc aacatttgaa acatctcttg ttcctattca
       61 attttcagac gtttacgtta ttcaattgtt acgtatcgca ttacttcttg tttcggatac
      121 ttcgtgatcc aaacatccat acgcattcac tatttatagg tttgtactct acacgcatgt
      181 ctgggtgaaa cctgtacttc taacaacttt atttgcttac actgttctat cgagaaattc
      241 ttctgtctat ttgatggtat cctccttcct tacatctata taatagcatc ctttggtagc
      301 gtttgcaaat ataagcactt taaattttac gctttcttaa caaataatcg ataagccctt
      361 tatttgaagt aaataaaaaa aggatgttat agtaaaataa gtaccttttt ttgtacgaag
      421 attttaaagg aggaatagga tggaagaata caagcagaca atagaagaag taaagaaggt
      481 aacagaagca gatccagata ctgggttgtc ttcacaagaa gtggatgaac ggcgaaaaaa
      541 gcagggattg aataaattcg atgaggcgcc gaaagaatcg atgatcaaaa aattctttcg
      601 aagcttatcc gattttacaa cgatcatcct tcttgtagca gcggtgattt ccttttatac
      661 cgcatttgct acagaacatg gggatttatt tgaaggactg ctgatcatcg ctatcgttgt
      721 gatcaactcg gtattggcga ttgtccaaga aggaaacgca gaaaaagcac tggaatcctt
      781 gcaagatatg aataaacaaa cggcaactgt catacgagac ggcaaagtga ccactgttga
      841 gtcagagcaa ctggtagtgg gtgatattct tgttttagag tccggtgatg ctatttctgc
      901 cgatgcccgt ttgatcgaag catcacaatt acgtgtagaa gaatcggcgt tgactggaga
      961 aagtgaagca gttgaaaaag atgcagcatt tattgctgaa gaagatgagt cattaggtga
     1021 ccaacttaat atggtattta aaggatgtac ggttgctgca ggtcgtggga aagcgattgt
     1081 cactgccatt ggtatggcta cagaaatggg taagatcgcg gatcttttga acgaaaatac
     1141 tatgcaaaaa acacctttac aggtccgatt gaatcagcta ggcaaacgaa tcagtatgat
     1201 cgctttagcg gctgcggctt tagtatttgt gattggtgaa ctgcaaggag aaccactcct
     1261 tgagatgttc atgacttcga tttccttggc tgttgcagct gtaccggaaa ccttgactgt
     1321 tattgttacg ttgacacttg catacggtgt gcagaagatg gcgaggaaac atgcgatcat
     1381 ccgacagttg ccagcagttg aaacgctagg aaccgctaat gttatctgct cagacaaaac
     1441 aggaacgctg acacaaaatg aaatgcgcgt ccgccgggta tggagcaaag aagatgaagt
     1501 aacgaatatc gaagattcaa tgacgaacag cgcgatggaa atattgaaaa tggctgcact
     1561 atgtacagat gtgacggtcg agcaagaaga tgacgacctt gttatcaaag gtaatccaac
     1621 agaagtagcc atcgtaaggg ctgttgaaga gaattatcat acaaaagcag aattagaaga
     1681 aaaatatcca cgagtaaacg agcttccttt tgattctgaa cgaaaaatga tgacaacagt
     1741 tcaccaaatg ggcgaaaaat acatctcaat caccaaagga gcatttgatg ttcttgctcc
     1801 gcgctttagt tcaggcgatg tagaacaagc ggcgatcgtt aatgatcgct ttggtaaacg
     1861 ggcactgaga gtgattgctg ttggttatgc tacttatgac gaagaaccgc aagatatttc
     1921 ttctgaagca ctggaaaaag acttacgttt gattggtctg atcggaatga tcgatccacc
     1981 acgtccagaa agtaaaggag caatcaagcg agcgaaaaag gctggcatca aaacagtcat
     2041 gatcactgga gatcatgtgg taactgccag tgccatagcg aaagaattag gtatcttgaa
     2101 tgatccgtca gaagcactct caggatcaga actgcatcaa ttatcagacg aagaattaga
     2161 tgcgcgtgtc aaagctctct ctgtctatgc tcgggtaacg ccagaagaca agatccggat
     2221 cgtcaaatct tggcaacgta cgggtgcagt cgtagcgatg actggggatg gtgtgaatga
     2281 tgcaccagcg ttgaaagcaa gtaacgttgg gtgtgccatg gggatcacag ggacagatgt
     2341 ggcgcaaagt gccgcagata tgattttgac ggatgacaac tttgcgacga tcgtagatgc
     2401 tgttagtcaa gggcgttcag tttatcagaa tattcgtaaa gcaatcaact ttttactaag
     2461 ctgtaatatt tcggagatct ttattgtttt gattgcgatg ctgttaggat ggggagcacc
     2521 atttacagct gtgcaactac tgtttgtcaa tgtagttgct gatggacttc caggatttgc
     2581 tttaggaaga gagccagcgg aacacggcat catggatcag ccacccattc cgaaaaatga
     2641 agggatcttt gctcgaggt
//
LOCUS       DACELF010000163         2648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.173, whole genome shotgun sequence.
ACCESSION   DACELF010000163 DACELF010000000
VERSION     DACELF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.173"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            708..2567
                     /gene="ltrA"
                     /locus_tag="IW687_002684"
     CDS             708..2567
                     /gene="ltrA"
                     /locus_tag="IW687_002684"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0951902.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1022 a          421 c          508 g          697 t
ORIGIN      
        1 ggttaggcac ttaaaagaag aaaacataag tttcctttta aggagaactg atattttgag
       61 cagtggaatg atggggtaac gccctgaaac gctcccttaa tactccgact ggctgtttaa
      121 catatagctg ttagatggtc agaagctcgg tgaagtcggc tgaaagttac cgtaaatatg
      181 ataaattcat atacgaaagt tgttcagagg tgaatgatgt aacctatagg tcggaggtct
      241 ataaaatact cacggtgaga atgtaagtaa tactactgac gaacttgcga atgaaagaat
      301 ctatacacgg actgtataga aatgtacagg attctaccgt atcaacggaa cagaatgtat
      361 gttgtggttt agtaagatta ttggttatga acaaccatgc aatgttacag gcattggaaa
      421 tatacaggtt caaagcaagc acctaaaagt atatataaag ataaaaatat cgaaacatgg
      481 gaagctggca atgtggagga aactatattc cattgaaaca tttgtgacga agaataataa
      541 gcacatttat gccagtgaaa gtggtggcac agtaccaatg aggatataga aatatatcgg
      601 aggaatagcc actagtcaaa agaaatcgtt aattaaatga tgtatttcat agaatcgagt
      661 aagacaaaat aaacactaca acctaagtaa acaaaggtag tggtgctatg aataaagtaa
      721 aaacaaataa aacacttcgt catagtgaat actatggcat gacacaaata tttgatgaac
      781 tgtatgtaaa aagtgataaa aaagaagttt ttacagagct catgacaatt attatgagtg
      841 atgaaaatat tcgactagca tacagaaata tcaagaataa caagggcagt tatacagcga
      901 gtaccgataa gaaaagtatt cgagacatta aaacactgtc agaagatacc tttctaactg
      961 aggttaaagt tctttttcgc tgttacaaac cacgagcagt tagacgtgta gaaattccca
     1021 aaccaaatgg aaaaacacgc cctctgggca ttccatcaat ctgggatagg ttaatacaac
     1081 agtgtatttt acaagtctta gaaccgatat gcgaagctaa atttcacaat tatagttatg
     1141 gatttcgacc aaaccgttcc acacatcatg cgattgctac ctgcaatcga ctggcaaata
     1201 gggggaagct acactttgta gtagatgttg atattaaagg attctttgat aatgtcaatc
     1261 actctaaact tttgcgtcaa atgtggacga ttggaatccg tgataaatgg ctattaggta
     1321 ttattcgatc catgctaaat gctcctatag tcctaccaac cggtaaagtc gaacacccta
     1381 aaaaaggtgt acctcaaggt ggtgtcctat cccctttact atctaatatc atattgaatg
     1441 aattagattg gtgggttgct tcacaatggc aaaataccac gattgagaca gaaaagatta
     1501 tggataggcg agataaaggc aaaggcattg ataagactaa tcagtatcgg gcattaagac
     1561 gaacaaactt aaaagaaata tacattgttc ggtatgctga cgacttcaaa atcttttgta
     1621 gaaaacgaag tcatgcagat aaaatatttc acgcaacgaa aatgtggctt aaagaacggt
     1681 tatcacttga aattagcgaa gaaaaatcca aaattgtcaa tctcaaaaaa ggtaaatcag
     1741 aatatctagg atttgaatta aagctaatca agaaaggtaa aaaatacgta gtcgaaagtc
     1801 atatgagcaa aaaatcaatg acaagagtta aaatccaatt aaagaaacaa ttacgtaaag
     1861 ttgccagacc gaagaatcat tgcgaacaag caaaagaaat tggattatat aattctatgg
     1921 tgattggaat ccatcaatat tatggaattg caacctgtgt aaatctcgat tgcagtaaaa
     1981 tagcctatac actaaaaccg ttcatcaatc acaaactgcc gacaaagaaa catggaaaga
     2041 tattaaatac atttatcaaa tcgaagtatg gaaaaagtaa agaaatgcga tggttgaata
     2101 atgtgccaat tgtaccgctc aactatgttc agtttagact tgctgtcccc ctatcagagg
     2161 gaacttgcaa gtacactgaa agtggacgaa gcaagataca cagtaaatta aaattagacc
     2221 tagccctttt acttcacatg atgagaaata gtaattatga aagaagtatt gaatttgtgg
     2281 ataacagaat atctaaatat tctgctcaaa agggaaaatg tgctatcacg ggtaaatttc
     2341 tcgaatacga agaaatccat tgccaccata tcgtaccagt aaaacaacag ggtacagaca
     2401 aatacagcaa cctcattatt attcacaaaa atgttcacgc attagtccat gctatcgaag
     2461 aaaaaatcat acacaaatac ctgaatgtat taaatctaac caatgaacaa atagaaaaac
     2521 taaatgaatt acgtgtcaaa gctggtaata gtgttttaac agtttaactt gaaaaatata
     2581 tcatacttaa cattcttttg atggaacgcc gtatgaagtg aaagtttcat gtacggtgtg
     2641 aagcgggg
//
LOCUS       DACELF010000164         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.174, whole genome shotgun sequence.
ACCESSION   DACELF010000164 DACELF010000000
VERSION     DACELF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.174"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW687_002685"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW687_002685"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0951903.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          941 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaa
//
LOCUS       DACELF010000165         2563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.175, whole genome shotgun sequence.
ACCESSION   DACELF010000165 DACELF010000000
VERSION     DACELF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.175"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1978
                     /locus_tag="IW687_002686"
     CDS             <1..1978
                     /locus_tag="IW687_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0951904.1"
                     /translation="QANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVELNAISPY
                     IQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAKNAYLTLD
                     QTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFGVDATNVT
                     LSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAESQTDISA
                     YLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQDYQKQLEA
                     TYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFATQMRRSPG
                     STIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVAQSLNLPA
                     VWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYSAFANDGQ
                     MYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTAAAANPAG
                     YTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGTSGNVVGK
                     VFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQDNLNQYGE
                     QAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            2046..2387
                     /locus_tag="IW687_002687"
     CDS             2046..2387
                     /locus_tag="IW687_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0951905.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT          902 a          479 c          592 g          590 t
ORIGIN      
        1 acaagcaaat gtcgaaacgt taaaatcagg attaagccaa tcgacagtca tttatgataa
       61 gaaaaacgaa gaagctggca cactgtacgc ccaaaaagga acatatgtag aattaaacgc
      121 gatttctccc tatatccaag atgcagtaat ctcgacagaa gataggaatt tttacgagca
      181 tcacgggttt gatatcaaag ggattgcccg tgcagcagtc cgaatggtct tgtctggcgg
      241 aacatcaggg ggcggaggaa gtacgatcac tcagcagctt gccaaaaatg cgtatctgac
      301 attagaccaa acgtttgatc gaaaagcgaa ggaattattt ttggcaatcg agatcgaaaa
      361 gaagtacagc aaagaagaaa tcttgaccat gtatcttaac aatgcctatt ttggcaacgg
      421 ggtatggggt gtccaagacg cttccagaaa gtattttgga gtagatgcaa cgaatgttac
      481 gctgtctgag gctgctactt tggcaggaat gctgaaaggt cctggtattt ataatccgat
      541 cgatcaccca aaaaatgcga atgatcggaa aaacacagta ctaagtgtaa tggaagaaaa
      601 cggaaaaatc acaaaagaac aagaacaagc agaaagccag acagatatca gcgcgtatct
      661 taatgacaca tacgataatt cagattctgg ttatcgttat ccttattatt ttgatgcagt
      721 gatcgatgaa gcagttaacg agtatggtct tgatgaagaa gatgtcatga ataaaggcta
      781 taaaatctat acagctttag atcaggatta tcaaaagcaa ttagaagcta cttatcaaaa
      841 cagtgcatta ttcccagcaa atgcttcaga tggtgccatg gtccaatctg cttcagtagc
      901 tttagatccg aatacaggtg gtgtacaagc actagtagga cgaagaggag atcatgtctt
      961 tagaggattc agctttgcta cacagatgcg tcgttcacca ggatcaacga tcaaaccatt
     1021 aagcgtatat acaccagcac tggaagctgg atacaagcct tccagtatct tgaaagacga
     1081 accgcaaagt tattacgatg cgcataattt tgacgggaca taccaaggag aagtaccgat
     1141 gtatcaagct gttgcacaaa gtttgaattt gcctgctgta tggcttttgc atgagatcgg
     1201 tttacaaaaa ggatatgata aagcagaaga attcggcctg cctttagcaa aatctgataa
     1261 gtattatggc ttagcccttg gtggactgga aaaaggagta tctccattga tcatggcggg
     1321 tgcttacagt gcatttgcca atgatggtca gatgtatacg ccgcatctta tcacgaaaat
     1381 cgttgattcg actggagcgg tgatcgtcga taacacaaac aaaaaaccaa aacaggtcat
     1441 atcaaaggaa acagccgatg aaatgaccag catgcttctt ggtacatttt caaatggaac
     1501 agcggctgct gcgaatcctg caggttatac gatagcaggg aaaacaggaa ccacagaaac
     1561 taatttcgat gccacaaaag caaatgacca atggatgatc ggttacacgc cagacgtagt
     1621 gatttcgaca tggatgggtt acgaagaatc cagcaagctg cactatttgg aagggacaag
     1681 tggaaatgtt gtcgggaaag tcttcaaatc agcggcagaa ggcatccttc cgtatacttc
     1741 aaaaacagga tttagtgtag cagatgccta tgccacagga ggacaagtgg tagctgcaga
     1801 tcaagttgga aacacaggaa attctaatgg agagtctgga aattggcaag ataacttgaa
     1861 tcaatatggt gaacaagcaa aagaagggct gaaaaacttc ggcgatatgg tgaaagaagg
     1921 cgttcaggga attggtgaag cggctaaaga cctctggcgg aaatatcaag gagagtagcc
     1981 cctttgaaaa cgaataaccc catgttacaa tagagagtaa ctactaaaag aaaagagggc
     2041 ataacatgtc aaacatctac gatagcgcga atcaaatcga acgtgagatc cgtgaattac
     2101 cagaattcaa agcgttgaaa gaagcgtacg agaaagtaca agcaaacgaa gaagcacaca
     2161 aactgttcaa agacttccaa gcgatgcaaa tcgaattaca agaaaaacaa atgagtggac
     2221 aagagttcag cgacgaagat gcaatgaaag cacaagaaat ggctgtgaaa atccaatcag
     2281 aagaagtaat caatgacttg atgcaaaaag agcaaggctt cagtacaatc atcaatgact
     2341 tgaaccgtat catcatgaca ccagttcgcg acctttacag cgaataaaac tattttatga
     2401 aacaacagag ggtgtgaaag agacgttcgg ccttgagcga tgacaaaatt gcttcttatt
     2461 gccgataggc tgtctcttga acaccattta ccaagaggtc gtgagcctac cgtttaactc
     2521 tgagttacaa ggaacaattt caaaaaacag cttctcatgt ttg
//
LOCUS       DACELF010000166         2557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.176, whole genome shotgun sequence.
ACCESSION   DACELF010000166 DACELF010000000
VERSION     DACELF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.176"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..711
                     /locus_tag="IW687_002688"
     CDS             <1..711
                     /locus_tag="IW687_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951906.1"
                     /translation="SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPR
                     LFDPTEGKVEVGGVDLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEM
                     EHASSIAQAKEFIEKLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDST
                     SALDARSEKLVREALDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELV
                     AGNRVYQEIFETQKGTEE"
     gene            714..2486
                     /locus_tag="IW687_002689"
     CDS             714..2486
                     /locus_tag="IW687_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951907.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT          829 a          438 c          564 g          726 t
ORIGIN      
        1 tcttttcgtt atcctggtga ggatacagat acgcttaaag atatcagctt ttctatcaaa
       61 cctggagaaa tggttggtat cgtcggggcg actggtgccg ggaagtcaac actagcacaa
      121 ttgatccctc gtctatttga tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt
      181 cgtcaagtaa atgaaaagag tttaagacaa acggtttcct ttgttttgca aaaagcaatt
      241 ttattctcag gaacgattgc tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa
      301 gagatggaac atgcttcttc tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat
      361 cgttacgaag caccaataga agaacgtagc agtaactttt ctggtggtca aaagcaacgt
      421 ttatccatta ctcgtggcgt tatcggtaat ccaaagatct tgatcctaga tgacagtacg
      481 agtgcactgg atgctcgctc tgaaaagctg gtccgagaag cactcgatcg agatttgaag
      541 ggaacaacga cgattgtcat cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc
      601 ttagtattgg atgaaggcag attagtcggc gaaggaaccc atgaggaatt ggtagctgga
      661 aaccgtgtct atcaggaaat ctttgaaact caaaaaggaa cggaggagta agtatgactg
      721 atctaataaa agcaagtaaa tttttctatc actatttgaa gcggtacaaa ctctcctttt
      781 tctttatctt tatcacagtg atcattgcta cctatctaca agtaaaggca cctcaataca
      841 tcggggaagc ttttcaagaa ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg
      901 ataaaagcaa atttgtttct gtaatatgga aactcttgct gttttatgta ctagctagtg
      961 cagccaactt tatctatagt attttgttca cacaagtagt agggaagtca acgaaccgaa
     1021 tgcgtatcgg tctattcaac aaattggaaa aattgacgat ccgtttcttt gattctcatc
     1081 aagacggtga aattttaagt cgttttacca gtgacttaga taatattcaa aatagtttga
     1141 accaagcttt gcttcaagtc atcacgaatg ctgttttact tgtcgggatc ttgatcatga
     1201 tgttccgcca aaatgtagag ttagcttggg ctacaattgc ttccactcca gtagccgttt
     1261 tgatagctgt cttgattatc cgcaaagcac ggaaatatgt ggatcttcag caagatgaag
     1321 tggggaaact aaatggttat atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa
     1381 atggcttaca agaagaaacg attgaaggat tcttagagca taatcaaagt gttcgcgaag
     1441 caaccttcaa aggacaagct tattctggtc tactatttcc gatgatgcaa ggaatgtcac
     1501 tggtcaatac agcgatagtt atctttttcg gcggctggtt agcattaaat ggtgatttgg
     1561 aacgttcggt ggcattaggc ttagttgtga cattcgtcca gtattcacaa caatattacc
     1621 aaccactgat gcagatttca tctggataca gcatgatcca attagcgatt acaggggcaa
     1681 gacgattgaa tgaaatattt gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat
     1741 ttgatggtgt tgaaaaaggt cttgcgttgc agcacgtaga ttttggttat tctccagaaa
     1801 aactagtctt gaaagatgtc tcgatcaatg tggacaaagg agaaatggtt gctcttgttg
     1861 gtccaacagg atcggggaaa acgacgatca tgaatctgct aaaccgtttt tacgatgtca
     1921 atgatggttc agtagtcatc gatggaatag atatccgaga ttatgatctt gatgcattac
     1981 gtagacatgt aggaatcgtt ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca
     2041 atatcacatt cggcaaaccg gacgcttcag atgaagaagt catcaatgca gcaaaacaag
     2101 cgaatattca tgacttcatc atgacgttag aaaaaggtta tgatacagag atatccgaag
     2161 aaaataactt attcagtaca ggtcaaaagc agttgatcag tattgctcga acgatcatca
     2221 caaatccatc tttattgatt ttggatgaag caacaagtaa tgtagacacg gtaacagaag
     2281 caaggatcca aaaagcaatg gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc
     2341 accggctgaa aacaatcttg aatgcagata gaatcgttgt cttgcgtgat ggggaagtca
     2401 ttgaagaagg cagtcatcat gagttattag cagaaaatgg attttatgcg gaactttatc
     2461 ataatcaatt tgtatttgaa agttaaatta aaagaacctc gttataacgg ggttcttttt
     2521 atatatttta tgttgtttgt ttttataagg ggcagaa
//
LOCUS       DACELF010000167         2515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.177, whole genome shotgun sequence.
ACCESSION   DACELF010000167 DACELF010000000
VERSION     DACELF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.177"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(493..1785)
                     /locus_tag="IW687_002690"
     CDS             complement(493..1785)
                     /locus_tag="IW687_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0951908.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IW687_002691"
     CDS             complement(2059..2319)
                     /locus_tag="IW687_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951909.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          847 a          463 c          380 g          825 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aaggc
//
LOCUS       DACELF010000168         2351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.178, whole genome shotgun sequence.
ACCESSION   DACELF010000168 DACELF010000000
VERSION     DACELF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.178"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..363
                     /locus_tag="IW687_002692"
     CDS             91..363
                     /locus_tag="IW687_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951910.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            808..987
                     /locus_tag="IW687_002693"
     CDS             808..987
                     /locus_tag="IW687_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951911.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1046..1402
                     /locus_tag="IW687_002694"
     CDS             1046..1402
                     /locus_tag="IW687_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951912.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          844 a          327 c          447 g          733 t
ORIGIN      
        1 ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt gtcggtagct
       61 gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa
      361 taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc
      421 aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa cgcctagtaa
      481 ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
      541 gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca tttgaacaag
      601 attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga tagtgcagct
      661 gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat cttttggcgg
      721 tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt tttcataggg
      781 ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa acggttaccc
      841 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtatggcaaa
      901 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
      961 tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat ataagtgata
     1021 tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt acaaattaaa
     1081 gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc atttgtatag
     1141 agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag ctggggatcg
     1201 atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc tttggcttat
     1261 tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact tagataagct
     1321 aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt tagaaagtga
     1381 cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat tattttatta
     1441 agaatgatgg aatataagga taaggttacc aacataacga tcattacttc tctgatatgc
     1501 tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc
     1561 ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta
     1621 tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa
     1681 gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga
     1741 catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat
     1801 gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa
     1861 gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa
     1921 atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt
     1981 gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac
     2041 atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg
     2101 attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa
     2161 tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc
     2221 aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga
     2281 tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt
     2341 aaaggttttt a
//
LOCUS       DACELF010000169         2337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.179, whole genome shotgun sequence.
ACCESSION   DACELF010000169 DACELF010000000
VERSION     DACELF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.179"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(110..475)
                     /locus_tag="IW687_002695"
     CDS             complement(110..475)
                     /locus_tag="IW687_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951913.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            683..1165
                     /locus_tag="IW687_002696"
     CDS             683..1165
                     /locus_tag="IW687_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951914.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1362..2252
                     /gene="pstS"
                     /locus_tag="IW687_002697"
     CDS             1362..2252
                     /gene="pstS"
                     /locus_tag="IW687_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0951915.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          815 a          377 c          466 g          679 t
ORIGIN      
        1 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
       61 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      121 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      181 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      241 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      301 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      361 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      421 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      481 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      541 atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta
      601 aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa
      661 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
      721 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
      781 tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga attttccagc
      841 tttgattgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
      901 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
      961 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattt
     1021 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
     1081 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
     1141 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaaacat atgagctgcg
     1201 taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg aaaaacgttt
     1261 acatgatttt tacataactt aactatattt ttacagcaac catacgaggg atttacagtc
     1321 gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa gtattgttta
     1381 ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga aatacagatg
     1441 gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc gttgcggtag
     1501 gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta caagaaaatc
     1561 caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc caagtcgaag
     1621 ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac ggagttgatg
     1681 cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc gtcaataaag
     1741 atacagatgt caaagatatc accaaacagg aattgattga tatctttaca ggaaaaatca
     1801 ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg
     1861 gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt
     1921 cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa
     1981 tcagctattt agctttctca tatctcgatg attccacaca agcactaagt atcgacggtg
     2041 tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca tatgaacata
     2101 tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac atgatgacag
     2161 aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca atgatggaag
     2221 tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc acggtagtcg
     2281 ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct ttaattg
//
LOCUS       DACELF010000170         2293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.180, whole genome shotgun sequence.
ACCESSION   DACELF010000170 DACELF010000000
VERSION     DACELF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.180"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..1598)
                     /locus_tag="IW687_002698"
     CDS             complement(51..1598)
                     /locus_tag="IW687_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0951916.1"
                     /translation="METTDTLLQLLQEAHKTNQTQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IW687_002699"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IW687_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0951917.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IW687_002700"
     CDS             complement(2043..2237)
                     /locus_tag="IW687_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951918.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          476 c          408 g          765 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg tttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACELF010000171         2276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.181, whole genome shotgun sequence.
ACCESSION   DACELF010000171 DACELF010000000
VERSION     DACELF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.181"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            56..883
                     /gene="panB"
                     /locus_tag="IW687_002701"
     CDS             56..883
                     /gene="panB"
                     /locus_tag="IW687_002701"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ0951919.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            898..1746
                     /locus_tag="IW687_002702"
     CDS             898..1746
                     /locus_tag="IW687_002702"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ0951920.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            1756..2151
                     /locus_tag="IW687_002703"
     CDS             1756..2151
                     /locus_tag="IW687_002703"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ0951921.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT          897 a          216 c          486 g          677 t
ORIGIN      
        1 atagagatta tagtgaatta gaaaagtatt taggagagag ttaggagaga aatagatgaa
       61 aaatacagca gttacattta aagaatctaa attaagaaat gaaaaattaa caatgcttac
      121 agcttatgac tattcaacag caaaaataat tgatgaagct gggataaatg gaatattagt
      181 aggagattct cttggtatgg tatgtctagg acatgaagat acactttcag taacaatgga
      241 agacatgatt catcatacta gagcagttac aagaggagct aagaacactt tagttgttgc
      301 agatatgcca tttatgtctt atcaaacttc agtatatgat tcagtagtta atgcaggaag
      361 attaataaaa gaaggacgag ctcaggttgt taaattagaa ggagggatag aagtatgcga
      421 taaaatagag gctatagtaa aggcttctat acctgttatg gcgcatatag gtttaacacc
      481 tcaatctgtt aatgcatttg gaggatttaa ggtacaagga aaggacaagg aagctgctaa
      541 agaattaata agagctgcta aagctgtaga aaaagcagga gcattcgctg tagtgttaga
      601 atgtgttcca actaagcttg cagagttaat ttctaaagag atatcaatac ctacaattgg
      661 tattggagca ggtgcagggt gtgatggaca aattcttgta tatcaagata tgttggggat
      721 gtatagtgat tttaccccta aatttgtgaa gaaatatgct aatttatcag aagagatgaa
      781 taaagcattt acaaaatata tagaggaagt aaaagatgga gtttttccag gtccagaaca
      841 tgggtttaaa ataagtgatg atgttttaga aaaattatat taaaagggtc aagaaatatg
      901 gttatattta aaacagtgaa tgatgttaga agtcaagtaa aagagtggaa aaaacaaggc
      961 ttaaaggtag gattagttcc tactatggga tatttacatg aaggtcatga aagtttaatt
     1021 agaaaagcat caaaagaaaa tgataaagtt gttgtaagta tctttgtaaa tccaactcag
     1081 tttgggaaaa atgaagattt aggtagttat ccaagagact tagaaagaga tatagaagta
     1141 tgcacaagag gtagagcaac agcaatattt aatcctgaag ttgaagagat gtattgtgac
     1201 aatgcaagta cttttgttaa tataactgga ttaacagaag gtttatgtgg agctagtagg
     1261 ccaatacatt ttagaggggt ttgcactgtt gtaagtaaat tatttaacat aataccagca
     1321 gatagagctt attttggaga aaaggatgca caacagttag ctgtaattaa aagaatggtt
     1381 agggacctta atattgatat agacgttgta gggtgtccaa ttataaggga agaggatgga
     1441 ctagctaaaa gttctagaaa cacatactta agtttagagg aaagatcttc agctactatt
     1501 ttaaataaat ctttaacttt agctaaggaa gctttaaata atggagagag agacagctta
     1561 aaaataattg agataataag taaaaatata aatacttata atttggcaaa gattgattat
     1621 gtagaagttg ttgattcttt atcattacaa agagtaaatt atattgaaaa atcagtttta
     1681 gttgcaatag cagtatttat aggaaaaaca agattaatag ataattttac atttgaatta
     1741 caataggggg ctataatgaa agttacaatg ttaaaaggaa aaatacatag agctacagtt
     1801 gaacaagctg agttagatta tgtaggaagt attactgttg atatgaattt attagaagca
     1861 gcaggaatat atgaatatga aaaagtacaa atagttgatg ttaataatgg aaatagattt
     1921 gaaacttata caatagctgg agagagagga acagggatga tttgcttgaa tggagctgca
     1981 gctagatgtg tatcaactgg agataaaata attttaatgg catattgtga attaaatgaa
     2041 aatgaggtta aggatcatag acctaaggtt gtttttgtcg atgataataa taaagttgaa
     2101 agagtaacat catatgaaaa acatggaagg ttatcagata taattcttta ggttttgtaa
     2161 tgttatttat atatagctag actattattg aaatatttat tgattactaa ttggatagat
     2221 gtcaataaag tagacacaaa aagagaagat acagactgta gacaaagtcc atttta
//
LOCUS       DACELF010000172         2237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.182, whole genome shotgun sequence.
ACCESSION   DACELF010000172 DACELF010000000
VERSION     DACELF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.182"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..445
                     /locus_tag="IW687_002704"
     CDS             191..445
                     /locus_tag="IW687_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0951922.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IW687_002705"
     CDS             446..763
                     /locus_tag="IW687_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0951923.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..>2237
                     /locus_tag="IW687_002706"
     CDS             1322..>2237
                     /locus_tag="IW687_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951924.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNK"
BASE COUNT          815 a          301 c          421 g          700 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaag
//
LOCUS       DACELF010000173         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.183, whole genome shotgun sequence.
ACCESSION   DACELF010000173 DACELF010000000
VERSION     DACELF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.183"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..390)
                     /locus_tag="IW687_002707"
     CDS             complement(94..390)
                     /locus_tag="IW687_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0951925.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IW687_002708"
     CDS             746..1102
                     /locus_tag="IW687_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0951926.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IW687_002709"
     CDS             1112..2011
                     /locus_tag="IW687_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0951927.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DACELF010000174         2221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.184, whole genome shotgun sequence.
ACCESSION   DACELF010000174 DACELF010000000
VERSION     DACELF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.184"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..410
                     /locus_tag="IW687_002710"
     CDS             <1..410
                     /locus_tag="IW687_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAQ0951928.1"
                     /translation="ENFGVTVAVEAGINHPIYNYQLAKRLIDEVASPNLKIILDCANL
                     MHKENHADQEKVIRGALDNLHGYITALHLKDYIMENGKIRMVPVGKGCLDFRPVLHYI
                     KYERPLMYATLEATPEIYVPEAIEHLQFIYQDC"
     gene            421..2175
                     /locus_tag="IW687_002711"
     CDS             421..2175
                     /locus_tag="IW687_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0951929.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT          674 a          436 c          430 g          681 t
ORIGIN      
        1 cagaaaattt tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact
       61 atcagttagc taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag
      121 attgtgcgaa tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg
      181 ccttggacaa tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa
      241 atggaaagat ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc
      301 attatatcaa atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt
      361 atgttcctga agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt
      421 atggaggaac gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg
      481 ctgcatcttt atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc
      541 aaacatcttc catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca
      601 tcgaaagatt cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg
      661 attggtcaaa atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt
      721 caagtagaag ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct
      781 tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct
      841 atccaaacgt tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc
      901 gtagctttag gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact
      961 gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca
     1021 gacttcaggc aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca
     1081 ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt
     1141 actcaaatct atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc
     1201 agctgggcta gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct
     1261 ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc
     1321 gtcaattccg tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct
     1381 atcacttcta ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa
     1441 aaaatccaac atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac
     1501 tcactcaaac cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct
     1561 tttgttgggc catctggctc tggaaaatct acactcctta acttattgat tggatttatc
     1621 cagccgactt caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga
     1681 agtgtgcgaa attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc
     1741 aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc
     1801 gaagctgcgc aactttcttc actgattgac cagcttcctg atggtctgga taccttagta
     1861 ggcgaacatg gcaacaagct gtctggtggc caaaagcaat gtatctccat tgcacgagcg
     1921 ttgatacgtc agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag
     1981 tcagagaaaa aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt
     2041 gtggctcatc ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga
     2101 aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc
     2161 ttgaaaaatg aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt
     2221 c
//
LOCUS       DACELF010000175         2117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.193, whole genome shotgun sequence.
ACCESSION   DACELF010000175 DACELF010000000
VERSION     DACELF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.193"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..384
                     /locus_tag="IW687_002712"
     CDS             <1..384
                     /locus_tag="IW687_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0951930.1"
                     /translation="KKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYRFTWKAEIN
                     HADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTEE
                     KQAEKRELSNKLKQDLLGNLQNLFD"
     gene            400..462
                     /locus_tag="IW687_002713"
                     /pseudo
     CDS             400..462
                     /locus_tag="IW687_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
     gene            1569..>2117
                     /locus_tag="IW687_002714"
     CDS             1569..>2117
                     /locus_tag="IW687_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0951931.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDIN"
BASE COUNT          716 a          336 c          422 g          643 t
ORIGIN      
        1 aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa tcttcaaagg tctgacaatc
       61 caaaagagat acggaaaagg acggggaaag cctgtcattg gttatcgttt tacttggaaa
      121 gctgaaatta accatgctga cgatttttcc aaagggaaac aagaagattt acgtataaaa
      181 ttatttaata ttgatcacaa tggagaatta acacgagaag aaaaatggcg cgctaaagat
      241 cgaatactaa atcttccctt aggcactcat gaagctgatt ttaaacaaca acaacaaact
      301 gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac tcaaacaaga tttactagga
      361 aatttacaaa acttattcga ttaagcttaa ggagtattta tggcagataa aacaatcaaa
      421 gaacttgcgg aagaattagg agttagtaaa acagcaatta acaatcatac taaattttac
      481 aaaaagtggc tagcattttc tagccacttt ttgtatggtg gagcgtgggt tttgaggggg
      541 attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat acggaggtat
      601 ttggctagca aatatagagc ttgccaaacc cttttagtga gttttgtaga aagtaccttt
      661 aaaggtactt tcacattacg aacgatacgt ccgtaggacg tactgaaagg gtgggactta
      721 tgagcaaatt aatgcaaaat ttggctagca aaaaagctaa aaaatatatg ctaataatcg
      781 agcgaaataa acactttttc aagtgagcga atccgccatg acaaagctga aataaactgc
      841 ttcagctttg tcatggcgtg tgccgacttt taaaaaagtc taggcacacg agcaaatatc
      901 tttatcaagc tctaatttgc gttttaagac gtttgaaacg cctaagtata taaatatata
      961 agaataggtt taagagtgct taaatcgaat tttagagggc atttagacaa ttttagggtt
     1021 gttagggctt gccctgaccg cctgtaaggc gttttaagaa caagataagg gatttatact
     1081 aggactgtct taaaatgtct taaacgaacg ctcagagctt ctcaaagggt tccttatgaa
     1141 cggcttttag caaaggagcc tttaggcgac tgcgcagtac aatgtgagag cgtagcgatc
     1201 acaccttttt ggcgaccgta ggaagcaagg ttttggattg gcagttttga aaaatgcgcg
     1261 aacctgttta aagaaaaaag aagtggtttt taggcgggaa aattagcccc cctcttttta
     1321 tatctctttt taaacctctt tttaaacctc tttaccgtcg gctgtatgct tactctccca
     1381 aggtgtgcag aatcttttgg gggtcttttt gtctgttttt aggggtcttt ttgtctgttt
     1441 ttaggggtct ttttgtctgt ttttgggggt ctttttgtct gtttttaggg gtctttaatt
     1501 aggggcttga cccctattag aaagctcttt ataattttgt taagtagtta atcttgaagg
     1561 aggattttat ggcaaatgaa attgtgaaat accaccatga attaaatacg attcctttac
     1621 gcaaatttac gtcagtagaa atgaatttat tcttctctat agtatctaga atgcgtgacg
     1681 taggcgataa aaaagtacaa tttacattcg aacaattaaa agatttaagc aattataaag
     1741 caactgcaaa tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt
     1801 tacgttttgg tagaagaagt gctgacggtt tgcaacgtga acgatttgtt ttatttaatc
     1861 aatttaaaat cgatggaaaa gctgatattc cttttgctga aatacaagtc catgagaaag
     1921 ccttaccctt acttaataat cttgaagaat gggttcgata ttctctccaa caattcaatg
     1981 aattagaaag cagttattcc aaaaccatgt ttagattatt aaaacaatac agaaccaaag
     2041 gaattgccta tttttcaaaa gaagactttc atgaattact tgatatccct aaaagttata
     2101 gagaaagtga tataaat
//
LOCUS       DACELF010000176         1986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.194, whole genome shotgun sequence.
ACCESSION   DACELF010000176 DACELF010000000
VERSION     DACELF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.194"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..1355)
                     /gene="glnA"
                     /locus_tag="IW687_002715"
     CDS             complement(15..1355)
                     /gene="glnA"
                     /locus_tag="IW687_002715"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0951932.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1383..1775)
                     /locus_tag="IW687_002716"
     CDS             complement(1383..1775)
                     /locus_tag="IW687_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0951933.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          565 a          438 c          336 g          647 t
ORIGIN      
        1 acggtattcc tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt
       61 tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt
      121 cctaatgcgt caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt
      181 gaaggtaagt cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta
      241 cggtcaacag ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac
      301 aatactgcca ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt
      361 gtggataaac cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat
      421 gcgacataaa caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga
      481 ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct
      541 gtttcactta gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac
      601 aaagacatat tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa
      661 gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata
      721 ttatcacaag catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct
      781 acttcatgat gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca
      841 cggcggcaat tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc
      901 aaggtcgttg tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt
      961 cctaaattga aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc
     1021 ccgcgaggat cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga
     1081 gcaacttttc catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac
     1141 aaatacatat cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc
     1201 attttatttt ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta
     1261 cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt
     1321 ttgatttctt cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt
     1381 attcagtcaa agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac
     1441 ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc
     1501 ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca
     1561 gccatattca atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc
     1621 agcgaataca tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca
     1681 taatagcgaa tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat
     1741 acggacatag atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag
     1801 ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac
     1861 atgagatctc tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt
     1921 tgattttcgt accagatacc ttttattttt tattatactg tactatcctt tattagtgtt
     1981 tttatt
//
LOCUS       DACELF010000177         1962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.195, whole genome shotgun sequence.
ACCESSION   DACELF010000177 DACELF010000000
VERSION     DACELF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.195"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..104)
                     /locus_tag="IW687_002717"
     CDS             complement(<1..104)
                     /locus_tag="IW687_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HAQ0951934.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDS"
     gene            847..1401
                     /locus_tag="IW687_002718"
     CDS             847..1401
                     /locus_tag="IW687_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0951935.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1655..>1962
                     /locus_tag="IW687_002719"
     CDS             1655..>1962
                     /locus_tag="IW687_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951936.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          758 a          212 c          331 g          661 t
ORIGIN      
        1 aagctatcga ttgtttgact gtacatttca taatcgggga tatcatcgac tttagtaaca
       61 ggattggcta agtcaaaatg ttcagtatat tttgatgaac gcatatgtag tcctcatttc
      121 ttgggagatt tgagactact atatgcgttt ttttgttgat ttaccaacat gtttagacat
      181 tttcctctag gaaaaaatta actagcacca cgggtatact actatatttt aggtttcaaa
      241 ttatataaat taataatata ctaccatttg ttggtagtat aatttaaagg taataagtta
      301 ttcttttatt actatgtttg ggttttatag caaggaaagt ttaataagag tttggatcta
      361 gcaaacaata ttaatgaggt cattaaatct tattatcttt agtgatataa cgaaatatct
      421 acacagacaa aattatgatc ttgcgtaaag ttataaaata tcaataaaaa tattttatta
      481 atgaaaagct tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg
      541 agttcttagt atattgttca aacttaaatt tcatataatt taattatgta aattagctat
      601 tctaagaact tctacggata attgcccgtc taaaatttaa ataagaggtt agaatcttca
      661 tataagtaag gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga
      721 tataaaatca taaactaaac cccttgattt tcttcacttt taacagagta tatatataag
      781 ttcatgatta tggtaaaata tatatataat catgaactca gaatattttt aagaagggaa
      841 acaatagtga gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag
      901 ttttggctaa gaagaacaag taatccagaa agagatagtt ttttattttt gtttggaatc
      961 aataatgggt tacgtatgtc agatattata ggattaaaag ttggagacat acgaggtaaa
     1021 agtaagccta ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat
     1081 ttgagagaag agattttatt atatactgaa ggtaaagaag aaaatgattg gttatttcca
     1141 agtcggcaac aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata
     1201 gcagaaaaat taggaagaga tgatatagga acacatacat taagaaaaac ttttgggtat
     1261 cactactaca agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg
     1321 caagcaatta caaaaaggta cattggtata actgaagatg aaataggggc atcgttacgt
     1381 ggttttaaat tgggaggcta attgagatat taggatatat tttttgtgta taatgaaaac
     1441 ttagaaggtg agattaatga agataactga attttcggaa gtatttaaaa cgtttagtaa
     1501 catttacaat agaataccag agatagaaca aaatgttgta gttgataatg attattatat
     1561 agagaatgat tttaataaac caagaaaata tgacaaccta tacaatttta tacacaatag
     1621 aacaacaata aataacaaca gtaacactcg agttttgatt aattcagcat attttcatat
     1681 taaatattta aagaaatatg agtatgaaga agttgtatta gattatgggc taaatagaca
     1741 gtcattttta atttatgcaa taaataacgg aaatttgcca agcaataaaa agaacataat
     1801 tattgaaaca tatgcctatc tggagggggg atctaaaaaa ctaattaagt catgtaatat
     1861 agagtatcaa ttatcagtag gagaaattaa atgtgttttc gaagaagatt tagaacttga
     1921 cttaaaaaaa atttttgcca gcattcatga ttatataggt tt
//
LOCUS       DACELF010000178         1926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.196, whole genome shotgun sequence.
ACCESSION   DACELF010000178 DACELF010000000
VERSION     DACELF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.196"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            339..1379
                     /locus_tag="IW687_002720"
     CDS             339..1379
                     /locus_tag="IW687_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0951937.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          736 a          255 c          306 g          629 t
ORIGIN      
        1 tagtatatat tactagtaat ggtagtatgt tttatatgca taaggtagtt cttaatttat
       61 tttttgtttt gacactggta gtacttaatt atctttaatt ttaaaactaa attttattca
      121 tacgtttttc tggtagtatt taattataaa taatatttat ttaaagtagt tcttaatgac
      181 tttaaaggta gtacataatt cattttattt ctgattttag gtagtattta attaaaaaac
      241 agttttaatg cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt
      301 ttacataaat aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta
      361 aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg
      421 acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat
      481 tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata
      541 cgacaaatga actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa
      601 aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag
      661 ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct
      721 acttactcga aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga
      781 aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta
      841 tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac
      901 ttaataataa tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac
      961 aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt
     1021 ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc
     1081 aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtggtattt
     1141 cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga
     1201 cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga
     1261 tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg
     1321 aaaaacgttt gaattatgaa agctccccaa gcactattac gaaagactgg ttagagtgaa
     1381 actttactga caaagtgatt gttgcttcat ggagtaacat taaaattaaa aattttttat
     1441 tattctaatt atattacgaa tattacaaaa taatagctat tttaatagaa tttataagtt
     1501 aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata
     1561 attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata
     1621 ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt
     1681 cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta attgtctgct
     1741 atgcttgtga tgaattgatt ttcgttctgc acttttggat gataaatttg gacaattgaa
     1801 aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca
     1861 tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg
     1921 caatgg
//
LOCUS       DACELF010000179         1881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.197, whole genome shotgun sequence.
ACCESSION   DACELF010000179 DACELF010000000
VERSION     DACELF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.197"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            153..1313
                     /locus_tag="IW687_002721"
     CDS             153..1313
                     /locus_tag="IW687_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0951938.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1329..1772
                     /locus_tag="IW687_002722"
     CDS             1329..1772
                     /locus_tag="IW687_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0951939.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          652 a          300 c          376 g          553 t
ORIGIN      
        1 aacaaaaaat atgagtagct atttttttgg gctgtactta ttacttgatg caggacagct
       61 ttttcacaac ctcatcggct ttcattttct atttttttcc tgaacccata taataaaaga
      121 aagaacaaaa aaacttgcaa gggaggacac atgtgaagca gagtaagcaa aaacagtctc
      181 gtcgactttt gtttcgtttg tttttattaa tatgtatcgt cgtagggtta ctgatcgctt
      241 tatatccatt ttatgtggat tctttaaata gttttattga tcaaaaacgt atcgaacaag
      301 tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa gaaaatggag gagaaaaacc
      361 aacgattaac tgaccaagga ttcaatcctg gtactgatcc tttcaatgaa aagaacagaa
      421 atgaaagtac aacatccagc caattagagg aatggctgat cggaagtatc aatataccta
      481 agatccaatt aaatatttca ctttatgatc ggctcaatgg taggatcctt gaaaatggag
      541 caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa ttcgacgcat tcagtgatct
      601 ctgctcacag cggtttacct aatcgacggt tatttaccga attagatcgt ttagaaaacg
      661 gagacacgtt tattttgact gttttggggg agaaattggc ttatcaagtt aaaaatatac
      721 aagtggtact gccagatgac acgtctgttc taacgatttc agaaggagaa gacttagtta
      781 ccttgcttac atgtacgccg tatatgatca acacgcatcg tttgctagtg acaggacatc
      841 ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa aggaaatcag gaaagaacca
      901 tgcggcaatg gtttattctt gcaggaacga taatagctgt agtcatactg ttagtgttta
      961 tcggacgact aatctatcag tatagattaa gtaaaaaagt gcttgatttt tcatttatta
     1021 ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt cattttgaaa cataagaaaa
     1081 aaacacttac tagaaatggt gtgccgtttt cagtacaaag cgaccatcat gggaaagtca
     1141 agctggatca attgcctggt ggaacttatc gaatcgtttc agctgatgat cctaaagttg
     1201 ctgtttcttt tggtattaga aaagtaaaac aagagaagat atactttttt gaggggcgaa
     1261 aacttgtaaa agagcttcaa aaaaatggtt tttattttaa aatcaatgaa taagtaagga
     1321 gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa agaactggaa tctgttctat
     1381 cgatttggct gcatgctaac ttggattctc atgaattcat tccatcaact tattgggaga
     1441 atcaagtaga atctgttagg aaagctttac cagatgcagt gatttactta gcagaaatag
     1501 atcaagaaat tattggtttt gctggggtga ttgatcagtt tatcgcagga atctttgtga
     1561 aaagggaata ccggcaacaa agaatcggta cgcaactaat gaaaaaaata aaagaaacca
     1621 ataaaacact gactttgaca gtctatcaaa aaaatcaatc agctattcat ttctatcttt
     1681 cacaaggttt tacaattcaa acacagcaaa aagatgaagc agtaaatgag gaggaacttg
     1741 tgatgaaatg gaatcaaaac gctgattttt gatgaacaga ataacgaaat aatgctccca
     1801 gcctaatttt ttgctgggag cattatttca ttttgaagtc actaatttaa ctgtgtgaat
     1861 atgcagttgt tacaatcttt g
//
LOCUS       DACELF010000180         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.198, whole genome shotgun sequence.
ACCESSION   DACELF010000180 DACELF010000000
VERSION     DACELF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.198"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(315..1607)
                     /locus_tag="IW687_002723"
     CDS             complement(315..1607)
                     /locus_tag="IW687_002723"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0951940.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          536 a          388 c          331 g          604 t
ORIGIN      
        1 tggagaggtt gtgaaaaagc tgctctgcat caagcagtaa gaacaatcag ataaaaatag
       61 cactccatat ttttcatctg attgggctta gtgccgtaga tgcaagcttt tgaacaccgt
      121 ttgttcggaa aaagggactg tgacacggtt tttgtcacag tcccttattc tgaataaaca
      181 gtgttcaaga gacagcctat cggcaataag gctaaacgtc tctttcacta cctcttcaag
      241 ttattattat cgtatagata aacacttcga ctcgctctgg taagaacgtc ctgattcagt
      301 cgtcgttttt atccttagat ttgcgcccag acgctttcta agatgtttgt ttgtgttcta
      361 tcaggtccga cagaaaatgt tgagatacgt acaccaacaa gctctgagat acgacgtaca
      421 tagttacgtg cgttttctgg cagttctgca agtgttttgc atcctgtaat gtcttctgac
      481 cagcctggca attcttcata gattggtttg cagcggttca actctttcaa gcttgctgga
      541 tagtggtaga ttagttcgcc atctaattca taagccgtac aaatttttac tgtttccaat
      601 ccacttaata catcgatcga gtttaaagat aggttggtaa tacctgaaac tcgtttagaa
      661 tggcgcataa ctacagaatc aaaccagcct acacgacgcg gacgaccagt tgtcgtaccg
      721 tattctcgtc ctacttcgcg gatttgttct cctgtttcat caaataattc agttggaaat
      781 ggtccatcac cgacacgtga cgtataggct ttgcagacgc cgacaacttt attgattttt
      841 gaaggcccta caccgcttcc gattgttacc ccaccggcta ctgggttaga agaagtaaca
      901 aaaggataag ttccctggtc gatatccagc attacacctt gtgcgccttc aaacaacacg
      961 cgtttgcctg catccaaagc atcattcaag ataacggaag tatctgtgac gtattgtttg
     1021 atttgttgtc cgtattcata atattcttca aaaatatcgt caaaatcaag tggttctgaa
     1081 tcaaacattt ttacaaattg acggtttttg tcatctagat tcaaacgaag acgttcttca
     1141 aaaatttctt tatctaataa gtctgcaata cggataccta cacgagctgc tttatccata
     1201 taggcaggtc cgattccttt aatcgttgta ccaatcttat tttcgccctt agcatcttct
     1261 tgtaattggt ccaatttgat atgataaggc aagattacgt gcgcgcggtc agaaatacgc
     1321 aaattacttg tttctacacc tttttcatgt aaataagcta attctttaac caatgattta
     1381 ggattgacta caacgccgtt tccgatcaca ctgatcttgt ctttataaaa aatcccagaa
     1441 ggaatcaaat gcagtttata agtcacaccg ttaaacttaa ttgtgtgccc tgcattgtcg
     1501 ccaccttggt accgtgcaat cacttcagca ttttcactga gaaagtctgt gatttttcct
     1561 ttcccttcat cgccccattg cgtacctact actactacag atgacatgtg aacacctcgt
     1621 tctatattat ttaatcctga accattgtat caataataga tacatatttc aaataaaatc
     1681 gaatgatttt tatttttatt ttataaaaac gcttaaaaaa cgaactttat ctttttagaa
     1741 tggttattaa aaaagaggtt gtaacagaag tggtcagctt caagaaataa gaaggaatgt
     1801 acgaaaattg cttctcaaat ttttgtgaaa tttcgactta tttttcgaag aagctactt
//
LOCUS       DACELF010000181         1827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.199, whole genome shotgun sequence.
ACCESSION   DACELF010000181 DACELF010000000
VERSION     DACELF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.199"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..132
                     /locus_tag="IW687_002724"
     CDS             <1..132
                     /locus_tag="IW687_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0951941.1"
                     /translation="STSPSTATFEQRNTATPNNNGLNASQNPFGGQSIDISDDDLPF"
     gene            177..410
                     /locus_tag="IW687_002725"
     CDS             177..410
                     /locus_tag="IW687_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951942.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(569..691)
                     /locus_tag="IW687_002726"
     CDS             complement(569..691)
                     /locus_tag="IW687_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0951943.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            881..1105
                     /locus_tag="IW687_002727"
     CDS             881..1105
                     /locus_tag="IW687_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951944.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1548..1754
                     /locus_tag="IW687_002728"
     CDS             1548..1754
                     /locus_tag="IW687_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0951945.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT          611 a          343 c          355 g          518 t
ORIGIN      
        1 tccaccagtc caagcaccgc cacctttgaa caacggaata cagcgacgcc taacaacaat
       61 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
      121 ctaccgtttt agtttgagaa agaaggagta agcatggcaa aaaaattagt ctgacgatgg
      181 atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc actgaaaatc
      241 ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat cttgtctgtt
      301 cttatctttt aaaaagagca ggaaaggata attccttttg gcgattgact aaagtcacag
      361 tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag agttaacggg
      421 aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag gagttgcgct
      481 aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag ttatttggaa
      541 ataaccgctg acctgctaaa gtagacggtt atttcttttt gtcattttta agcatattta
      601 caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt tgaattgtct
      661 catatgcgga caaaaggctt actcctttca tagattttag cacttacatt cataagcacc
      721 accacctttc cagaagtcgc caccgtcttc aaactttact gtcttgacta ttatagcata
      781 tctaaaatcg aataaaaatt ctcaatttta gctgtttttg ctataatttt agcgaggtga
      841 tcgtgatgga acaatatatt ggtttactcg ataaattagg gtgatcaaga cacagccatt
      901 gcttgtgcga tttacattac gcacgagtca tgaaccaatc aattgtgtag tggccaatat
      961 agaaatagcg gataaattgc tgattttgga agaaggcaag tatactattt cagtgacagg
     1021 tcacttcaat aaacgaaagc aactagtcat tgaatcaatg caaattagaa accctgatca
     1081 ttttacgcga tcgatgagca tataaaagaa gagctgcagt cctaaatgag gattgtagct
     1141 ctttttgttt tacatagaaa cagtaaaaat gaaaaggcct gtttaggaag gcacaagcgc
     1201 cttctcaatt gaaagacctt tctctcactc caaccgccac cgccgttcga gaagaaagga
     1261 agtgaacgct acgcgttcac taagataaag ggtcttgcat agcaagacaa aatagaaaaa
     1321 atttcctctc accaaaaggt tcgctcctgt tcgttatctc tttattgatt tgtcagaccg
     1381 gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg
     1441 cgcccttgtc ttgtcgcttt tcgttgattc attctggcca acaaatcaat aaagaaagaa
     1501 ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga atcaatcatg cgaaagaaat
     1561 ttgattattg gggtgttccc ttcagcgaat tattcccaaa ctatcatgca ccacatactg
     1621 tcgaatgcga ttgtggagaa agagcgaaat gtatcaaaag ctattgtttg tatcaatgtc
     1681 caacatgcgg gaaaaaatat accttgtctt atggcgatta tattttaatt gatgagaaag
     1741 gaaagaaacg ctgatgaaaa cacaacacaa attattgact gtaaaagaag tcgttcaaat
     1801 tttggtggac aaagaagtca tttctga
//
LOCUS       DACELF010000182         1818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.200, whole genome shotgun sequence.
ACCESSION   DACELF010000182 DACELF010000000
VERSION     DACELF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.200"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            227..565
                     /locus_tag="IW687_002729"
     CDS             227..565
                     /locus_tag="IW687_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951946.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(770..1009)
                     /locus_tag="IW687_002730"
     CDS             complement(770..1009)
                     /locus_tag="IW687_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0951947.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(985..1332)
                     /locus_tag="IW687_002731"
     CDS             complement(985..1332)
                     /locus_tag="IW687_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0951948.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          565 a          367 c          301 g          585 t
ORIGIN      
        1 tgtaaactat ctagaaaatt gttttgttta aagtatatct tattggatat aaatagaaat
       61 aaattgtagc cagttcattt tttctttaac taaaaaaata atccaagaaa cattgaacct
      121 atggatccaa aagctaccca aatactatag ctaacccccc gctaccttca attttgcaga
      181 taaaacgctt tgggagaaaa tttcaaagac aacaggccat cctatcatgc ccgattataa
      241 acaaaaagct ggctattcct tttgctctga caaatttcaa ttgggaaagt tcacgaaact
      301 agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt
      361 ggttcaacga atttcaacta gacaagactt tgctattgta gaacgtgaga tcgtagctat
      421 ttttgtagaa gaagggattt tacatgatcc aactcatatc aatgtggata aatggcaacc
      481 tcttatctat aaattcagag aatatacaac tgccaaccag tcgttaactt tgaattttag
      541 attccaagaa ttcaaagagt cataatgata aacatcgtta ggtataaata aaaaagactc
      601 gcagcacgag taaaaaacaa atagaagcta tacattagaa aggtcgactg tgaccctata
      661 gatgcagtat atgcagctta tgagtatttc ttctgttctc tttgtttttg ggatctgtgc
      721 tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa tcgattatgt tataccttct
      781 ccattactaa ttcaggcatc tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta
      841 cccaaggttc gatttgttct tcagtcaaga caagcggcat tcggtcatgg attggtgcga
      901 ctgtgcggtt aggttcagta gtcattatga ttgattctgc cattttttca tcctgatggc
      961 tacgataaaa accgccaata tacactattt tttccttatt tgaaaacata aatttgtctt
     1021 tttcttcatt ccactcattg ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt
     1081 ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt ttttaaatcc
     1141 ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt ttcttaacgt
     1201 cacaacaaga tttgacggaa aaacttctcc agctgctaat tccgctgcct gcttcggttg
     1261 tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat caaaaaaata
     1321 acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc aacaagaagt
     1381 tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac aagtgacaaa
     1441 ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     1501 acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac
     1561 gacctctggg taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     1621 aaatgtagaa agctattttc acaaaatttt tcttaatact cgaaactaag cgactttttc
     1681 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
     1741 cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt
     1801 tttcacaacc tctccact
//
LOCUS       DACELF010000183         1792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.201, whole genome shotgun sequence.
ACCESSION   DACELF010000183 DACELF010000000
VERSION     DACELF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.201"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW687_002732"
     CDS             <1..156
                     /locus_tag="IW687_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0951949.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IW687_002733"
     CDS             175..1452
                     /locus_tag="IW687_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951950.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..>1792
                     /locus_tag="IW687_002734"
     CDS             1462..>1792
                     /locus_tag="IW687_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0951951.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADE"
BASE COUNT          755 a          155 c          296 g          586 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aa
//
LOCUS       DACELF010000184         1701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.202, whole genome shotgun sequence.
ACCESSION   DACELF010000184 DACELF010000000
VERSION     DACELF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.202"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..255
                     /locus_tag="IW687_002735"
     CDS             <1..255
                     /locus_tag="IW687_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951952.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="IW687_002736"
                     /pseudo
     CDS             258..411
                     /locus_tag="IW687_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="IW687_002737"
     CDS             434..1639
                     /locus_tag="IW687_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0951953.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DACELF010000185         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.203, whole genome shotgun sequence.
ACCESSION   DACELF010000185 DACELF010000000
VERSION     DACELF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.203"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1297
                     /locus_tag="IW687_002738"
     CDS             128..1297
                     /locus_tag="IW687_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0951954.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          566 a          254 c          258 g          500 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataa
//
LOCUS       DACELF010000186         1564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.204, whole genome shotgun sequence.
ACCESSION   DACELF010000186 DACELF010000000
VERSION     DACELF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.204"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..1517
                     /locus_tag="IW687_002739"
     CDS             51..1517
                     /locus_tag="IW687_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0951955.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          290 c          330 g          510 t
ORIGIN      
        1 gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt atggaaaaac
       61 agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga
      121 tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat atgtacgata
      181 cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct
      241 acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag
      301 gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg
      361 ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg
      421 cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc
      481 gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac
      541 cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg
      601 ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg
      661 gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta
      721 acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc
      781 atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt
      841 attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat
      901 gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt
      961 catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga
     1021 tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat
     1081 tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc
     1141 ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg
     1201 gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg
     1261 ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat
     1321 ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg
     1381 cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg
     1441 cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa
     1501 tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc
     1561 tgtt
//
LOCUS       DACELF010000187         1549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.205, whole genome shotgun sequence.
ACCESSION   DACELF010000187 DACELF010000000
VERSION     DACELF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.205"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1361
                     /locus_tag="IW687_002740"
     rRNA            <1..1361
                     /locus_tag="IW687_002740"
                     /product="23S ribosomal RNA"
     gene            1455..>1549
                     /gene="rrf"
                     /locus_tag="IW687_002741"
     rRNA            1455..>1549
                     /gene="rrf"
                     /locus_tag="IW687_002741"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          432 a          331 c          458 g          328 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaaagaact
     1381 cggaaaaaaa cttatgtgac ttcaaatcca gttttgagtg aacaaaattt actcaataaa
     1441 agataacacc acagtgtggt ggcgatagcg agaaggatac acctgtaacc atgccgaaca
     1501 cagaagttaa gcttcttagc gccgattgta gtgaggggtt gccccttgt
//
LOCUS       DACELF010000188         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.206, whole genome shotgun sequence.
ACCESSION   DACELF010000188 DACELF010000000
VERSION     DACELF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.206"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..289
                     /locus_tag="IW687_002742"
     CDS             <1..289
                     /locus_tag="IW687_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951956.1"
                     /translation="LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVL
                     FVPENSPEVSEAIETLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            279..1145
                     /locus_tag="IW687_002743"
     CDS             279..1145
                     /locus_tag="IW687_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951957.1"
                     /translation="MTIKREKWLRGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSILWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAV
                     VTVLFVYLAARQFFSIRLLSRNTRFQSLTTVLLICFLTFSAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSG"
     gene            1242..>1530
                     /locus_tag="IW687_002744"
     CDS             1242..>1530
                     /locus_tag="IW687_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0951958.1"
                     /translation="MTLKKQAFLPYPYGNTTLTDFSLASGQTLYYLPESQRFIPVPNE
                     STRSATLQTERLVFTREELKSSQLKADDLENQQTNLQLPKSLPKRIEDLAVE"
BASE COUNT          461 a          296 c          287 g          486 t
ORIGIN      
        1 acttctgata ttaggcaacc gcgcctttca tggcaaagac cccggtcata cctattttgc
       61 ttctttggaa gctggcgatg agaaaacttt tttcgcacac ttgaaaaaat atgcaaaaaa
      121 acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt
      181 gtcaaaagat cgaacggttt atcttgttta tttcgtagaa aaacaactca tggtccaaga
      241 aaaagacaag atatgttcat tgccaggagg tgaatggatt gacgattaaa cgggaaaaat
      301 ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg tgctccgata tttgtagtag
      361 ctaatcaatt gcaaaacagc taccttctta tcctaactat tggtgtccta tgccttattg
      421 tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta atgataggtt
      481 gtttgttttt atattttcct tcgaagacat tttccatatt atggatctta cagttttttt
      541 cgtcagttac agaggaattc ttccatctat cccaaggtaa actttattat ttgccagaaa
      601 agacagcttt ttttctcata atgttgctga tctatttatt tattacattg accgtggatt
      661 acgactattg gtacgcaccc tttctttctt tgatggctta cttcatgatg cttaccgttt
      721 ttcggaaaaa agatttactg tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc
      781 tggcagcaag gcaatttttc tccatccggt tgttgtcgag aaacactcga ttccagtcat
      841 tgactacagt tctgcttatt tgtttcttaa cttttagcgc tatcttgcct ttatatcttc
      901 cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat ccgagaatat cttaatgatg
      961 aagggcttta tgacacactc catgagtatc aattaaccgg aagtgcaaga acaggcttca
     1021 gtgagaatga tgaacagctt ggtggacctt tatatgataa tggcgaagtt gtttttacag
     1081 cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa aaatcattac accggttctg
     1141 gatgaataga agatcaggac gcacccatcc aaccaatcca agaattccct gttgacatca
     1201 cgagctacca aaccgttata gaagacaaag aaaacattac attgacccta aaaaaacaag
     1261 ctttcttacc ttatccttat ggaaatacta ccttgactga tttttcatta gctagtggac
     1321 aaacgcttta ctatttgcct gaaagccagc gattcattcc tgtcccgaat gaatcgacac
     1381 gttctgcaac tttacaaaca gaaaggttag tttttacgag agaagaatta aaaagcagtc
     1441 agttaaaagc tgatgattta gaaaatcagc agacaaatct tcaattgcca aaaagtttgc
     1501 ctaaacgtat tgaggacttg gcggtcgaat
//
LOCUS       DACELF010000189         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.207, whole genome shotgun sequence.
ACCESSION   DACELF010000189 DACELF010000000
VERSION     DACELF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.207"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            119..382
                     /locus_tag="IW687_002745"
     CDS             119..382
                     /locus_tag="IW687_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0951959.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            382..654
                     /locus_tag="IW687_002746"
     CDS             382..654
                     /locus_tag="IW687_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0951960.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            731..1060
                     /locus_tag="IW687_002747"
     CDS             731..1060
                     /locus_tag="IW687_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0951961.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1050..1337
                     /locus_tag="IW687_002748"
     CDS             1050..1337
                     /locus_tag="IW687_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0951962.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          558 a          190 c          293 g          468 t
ORIGIN      
        1 aggaaacgga taacacttta caatgctgtt ttggtactcc aatttaaaca aaacaatcga
       61 atttacgtac ttaattaagt acgttagaat ggagatataa attagagggg gagtagatat
      121 ggcaaaaaca gtcttaaatc ctagctcagc acggaaaaat ttttataagt tgttgaaaga
      181 tgtaaatgag aatcatacgg aaattgaaat cattagtgag cgcagtgaaa ataatgcggt
      241 gcttattggt ttggatgatt ggaaagcaat caaagaaacg ctattacttg agcaaatagg
      301 cactttgggt gttgttcgta agcgagaaaa agatgatagt ggatttacta atgttgatga
      361 aattgattgg gataacttat aatgaataac tattctgtga tgattaaaaa ctcggctaaa
      421 gctgatttaa agaaaatcaa acagtctaat ttgaaacctc aatttgagaa ggttattcag
      481 actttaaagg aagatcccta tctccctacc caatcatttg agaagttaaa acctacacat
      541 gaaggtaggt attctagaag attgaataga caacaccggg ttgtttataa ggtagatgaa
      601 gatgaacacg tggttgaaat ttattcagca aggacgcatt acgagtcaca gtaattactt
      661 gtaaatacat tatctcgtgg tataatgtat ttactaagta attacatact gttagaaatg
      721 agggatttaa atgattacaa aaacaagaaa acaaggcaat tcaatcatgt tgacagtacc
      781 taaagatttt aatgttccta atggagtcga agttgaagca aagttggtag agaatggtat
      841 tctttatgaa tttgttgaac cacaaaaaga attttatgat tttagcgaag atattttgtc
      901 tgatataatt gcagaaggct acgataaaga tgagattctt gtagaattta agaatagaaa
      961 aaacaaaatg cattcttcct ttagagatat tgctgaagat acacttacta attctaaagt
     1021 aatgactaaa gaggagttgg caacagaaat tggcttatga aatcgttcct tcaaaacatg
     1081 tcattaagta tttaaaaaag ctaaaagaga agccattgaa agaaaaattt ttgaatttaa
     1141 tttatgatga aattgcgatt gatccatata agggtgaaca aaaaactggg gatttatcag
     1201 gtatttggtc tagtgggttc aaatatgcgg gcacaacata tagagtagct tacgagatta
     1261 tagaaaacaa ggttattcca gtattgttat gtggaacgca tgaaaacttt tatgaacagt
     1321 tgaaaaaatt aatctagtga tttgttgtcg ttcttaagaa ggacggcttc ttttttatcg
     1381 aaaatctgat tcgtctacat gactaaagtc gcgagtattc tctgcttctc gataaattaa
     1441 ataacgtcat tactttgagg aaagctataa atcaatatat ttcagctttt ccttatttat
     1501 gtactacct
//
LOCUS       DACELF010000190         1500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.208, whole genome shotgun sequence.
ACCESSION   DACELF010000190 DACELF010000000
VERSION     DACELF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.208"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            8..514
                     /locus_tag="IW687_002749"
     CDS             8..514
                     /locus_tag="IW687_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951963.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            501..953
                     /locus_tag="IW687_002750"
     CDS             501..953
                     /locus_tag="IW687_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951964.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            946..1344
                     /locus_tag="IW687_002751"
     CDS             946..1344
                     /locus_tag="IW687_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951965.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          587 a          179 c          262 g          472 t
ORIGIN      
        1 tagtgagatg gacattaagt ggttggttca acaaaatgat agcaatttag aattggcgat
       61 taaatatctc gaagaaacta tctttgaaga tgaacatctt acagataatt ttttacaagt
      121 attaaaatat cttgaaattt actctgttaa aaaaaataaa ttaattggtg aaaatgattc
      181 tccaataaaa acacctattg agctaagctt aagaaatcgg atgggaatac tccaaagatc
      241 ggaaatagta aaagaacttt tttatcataa atttagttat gaaataagac tagatgatac
      301 ttatgagcat tatagaattg tttttttcgt atacaattct atagaagatg ctactgcgac
      361 tactgcatta acattcggtt ttacgaaaaa tggaacaatc aattcggata aaacccgtca
      421 agctgctaca gaaagtgatg atatatgtaa gaaagtatgc aatggggaag agaattactg
      481 gataggggag gaaaagttga atgaaatcta ttaatgattt attaaaaaaa catcctgatt
      541 tatataaaaa attaaattct gaacaaagaa acctgtccaa acaattactt aaaataagat
      601 taaaaaaaca aaaaaacgtg acagaattct caaaaatgtt aggaataagt gaaaaaaaat
      661 atcttgaatt tgagttcgga gatatgcata ttcctgttgc agagtacaat aaacttttga
      721 aacaagttga aaatttgtcc tataaagatt caatgaattt cactattaag gttgctggcg
      781 gccctattaa atatgaccgt attgaaaata aaccttatat tcggaataaa agttactcag
      841 tatttaaaag tagaaagaga aaagaagtat cgtcaaatat ggtctatgct agtgttaatt
      901 accaagatac gagccgctat attgtagaaa aagaggtata tgtacatgga taaaggaaat
      961 agtgttactt ttgcacttga aaatattagt ttgaataaaa ttcattttga attaactgat
     1021 aattttagtt atggagattt agagtttaat attgaacttg gatataaaga agacgaagca
     1081 gagtatctag aaaaaaaact tgtttcttta cgagctcaca ttgtaggggg gatggaagga
     1141 gcaccggtag atattttact cttttcagtt attcgtgttt ctacaaatga acagccatat
     1201 aaaaaattag tagaagaatc tatgggagag ctagctaaac ctatcctgaa taaagcttct
     1261 aatttgattt ctaatttata tagcgaagta ggagttatgc ctacagttat tgatttatat
     1321 cagctatata gtcaacaaaa ataaaataca agtaatagag ttatctattt aaatttgcaa
     1381 gacaaagacc tcaaccagtc atccttggtt gaggcttttt tgctctgtaa ttaactcgta
     1441 tagatcctgc aagatagttt gtttctgaat aaatgattga tatgaagtat ttagatgact
//
LOCUS       DACELF010000191         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.209, whole genome shotgun sequence.
ACCESSION   DACELF010000191 DACELF010000000
VERSION     DACELF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.209"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW687_002752"
     CDS             <1..226
                     /locus_tag="IW687_002752"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0951966.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW687_002753"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW687_002753"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IW687_002754"
     CDS             533..1336
                     /locus_tag="IW687_002754"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0951967.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACELF010000192         1439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.210, whole genome shotgun sequence.
ACCESSION   DACELF010000192 DACELF010000000
VERSION     DACELF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.210"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..498)
                     /locus_tag="IW687_002755"
     CDS             complement(<1..498)
                     /locus_tag="IW687_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0951968.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYN"
     gene            complement(680..1336)
                     /locus_tag="IW687_002756"
     CDS             complement(680..1336)
                     /locus_tag="IW687_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0951969.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
BASE COUNT          447 a          282 c          180 g          530 t
ORIGIN      
        1 gttataagtc tgtgttttac aaagccttaa ctttatttcc ctgtctatgt tgcccaaaag
       61 acgaacccca tttcctaaca gagtaggaat aacagaaatg taatatttat caatcatatt
      121 tttgcttacc aactgctgaa caagatttgc tccgccacaa atccaaatat cctttcctgc
      181 ttcttgtttt agccgttcca ataataaaac aggattttca cttgtaaaat gaatttgttg
      241 ggaagaattg cactctttat gtgttaatac ataagttgta aatttattat atacccattc
      301 ttgcggagaa agttcagtaa caacctgatg agaagtattc caccccatta agacagtatc
      361 tatatctttg gtaaattctg tataagtgtc gatgttttca ttgtcatttc catgaccatt
      421 tagccaagct actccaccat taccgtccgc aatatatccg tcaaggctca tggcaataaa
      481 taaaactact tttctcattt tttctctttt ataaatacaa acaggaattt atttctcttt
      541 tttactcata agggaacaac tgattgctac aaataaaccc attaaaatcc atggaaatgc
      601 tgctcttata aaatcactaa ccatacttta ttcctcctta aattccaatt tccttagttc
      661 tacaaactga aatttttgcc tatgaatgta tcttgttctt tatacgcaaa caggcttctg
      721 tgtgcataat ccaatgtctt gaaaacggca tttgaattag tccacaaata tctttattac
      781 accaataatc aatcagccaa attgcttttt cattgtcgtt tactacattc aacgattcta
      841 agtatccttt tctttctttc ggtatttttc ttttcaattc atcataagat aaccgttcaa
      901 gcattttctc agtgctttcc catacttcga aaatatataa atataattct tccagattaa
      961 gctgttttga aaagtctgca atctgctgtt tcatgagttc atttcctgtt gtaatgatag
     1021 gtgaattgat acgctcttga taattgcctg caaaaaatac ttgttcatct tcatttatca
     1081 ctgtatgcac aactatatct tcaatgcgga aaatatgcca aatagaataa gcaatcgtct
     1141 tactatgata accatcagcg tttatgaagg ggattgagtt aaaatcttct ctgcataatt
     1201 cttctttgaa agatactaag gtttgtatta actgacttcg taaagtaagt aaagtatcaa
     1261 tacccctctt ataagtatct ttctttttta tttgtgcctg cattgtttta ttaagttcag
     1321 accattcctt attcatattc aatcctctct caaattctga ttttatcgcc ccatacaaac
     1381 tggaatttgt cttacaacaa tttatatatg cataaaaagc aatatattta tgaaaacac
//
LOCUS       DACELF010000193         1429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.211, whole genome shotgun sequence.
ACCESSION   DACELF010000193 DACELF010000000
VERSION     DACELF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.211"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..542)
                     /locus_tag="IW687_002757"
     CDS             complement(<1..542)
                     /locus_tag="IW687_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0951970.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFL"
     gene            complement(666..1262)
                     /locus_tag="IW687_002758"
     CDS             complement(666..1262)
                     /locus_tag="IW687_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0951971.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          560 a          254 c          231 g          384 t
ORIGIN      
        1 aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc aaaaataaaa
       61 tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa acttaatccg
      121 ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat gggtctcact
      181 ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg ttctttcttt
      241 ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa cagtaaaatc
      301 gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa aattgaagga
      361 atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg ggtcgtactg
      421 ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac atcacggaaa
      481 taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt caaataattc
      541 atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa agggtgcagt
      601 gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat tttacaaaat
      661 aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc catactatat
      721 aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt cactgacatg
      781 tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag aaaaatagct
      841 agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata tttgttatat
      901 gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc tagcagaaaa
      961 atgataatcg aaccaatgca gattccaata tagttataga taaatccagc ataagggcca
     1021 aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat aactacttgc
     1081 aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc tactaatcct
     1141 cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt cagcgcgatt
     1201 gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc agcaatattc
     1261 attattcatc aactccttag aggttctttg ttaactatac tttactagaa aagcaactaa
     1321 ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc ataagtataa
     1381 aagattgatc tcaaaaaaac atccaactaa agacttattt aataacttg
//
LOCUS       DACELF010000194         1420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.212, whole genome shotgun sequence.
ACCESSION   DACELF010000194 DACELF010000000
VERSION     DACELF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.212"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW687_002759"
     CDS             complement(862..1203)
                     /locus_tag="IW687_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0951972.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1394)
                     /locus_tag="IW687_002760"
     CDS             complement(1203..1394)
                     /locus_tag="IW687_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0951973.1"
                     /translation="MVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEA
                     EYYEKDEWEDLTPEGREIL"
BASE COUNT          449 a          230 c          205 g          536 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt
//
LOCUS       DACELF010000195         1403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.213, whole genome shotgun sequence.
ACCESSION   DACELF010000195 DACELF010000000
VERSION     DACELF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.213"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            92..1387
                     /locus_tag="IW687_002761"
     CDS             92..1387
                     /locus_tag="IW687_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0951974.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          276 c          265 g          412 t
ORIGIN      
        1 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
       61 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      121 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      181 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      241 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      301 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      361 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      421 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      481 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      541 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      601 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      661 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
      721 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
      781 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
      841 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
      901 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
      961 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1021 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1081 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1141 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1201 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1261 cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg cgtacggctt
     1321 tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa catttttaac
     1381 caaataaaca gaacagaaag agg
//
LOCUS       DACELF010000196         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.214, whole genome shotgun sequence.
ACCESSION   DACELF010000196 DACELF010000000
VERSION     DACELF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.214"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            581..994
                     /locus_tag="IW687_002762"
     CDS             581..994
                     /locus_tag="IW687_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0951975.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            1131..1391
                     /locus_tag="IW687_002763"
     CDS             1131..1391
                     /locus_tag="IW687_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951976.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT          459 a          215 c          227 g          494 t
ORIGIN      
        1 attatttgtt cacagcttcc agcaaacaaa tccgttccca aggaggatct ggaagcgttg
       61 gtttttatcg agtgtcaccc gaaggatcga ttacgatgac agatgcaaat ggaacaccat
      121 tttgattaga tgatattata cggatttaca gtactagcaa gtcattattg gtatcatttt
      181 ttctgagaaa gttatacgga gtataaataa gcatttgcat taacctaata attcgttaca
      241 gctatttctc ttatgaatat actcacttct ctacttgctc tcttgataaa tttttggtat
      301 ctacttcatt ttcggtcaac tgagttaatg aaatttattg tcgggcggtt ttctagattc
      361 aatttttcta tatttggata gaagaattta cttttttacc ttttgacata ttttcttctt
      421 attattttaa tgatcccttt ttcaaattag aaagatagcc tttgtatcgt attagatgaa
      481 atattcacaa agctaaccat tatacttgca tttttttcaa aatagagtta ttacgtaaat
      541 agggataaat aaaaaatact tattgaaagg atgatcaaaa atggcaaatg tacctagcat
      601 tcgcgacatg ttccctgatt tcaatgacgt gtttagtcca gcattcaatg attttttagg
      661 tgtttcaagt tatccaaaag tagatttagt cgaaaatgaa aaagaataca aactaactgc
      721 tgatatgcct ggctgtgaca aagaagacac gacagttgaa tattcagata atactttgac
      781 gatctccgcc aatcatgagt cccatacaga agataaagaa gatggcaact atgtacggaa
      841 agaaagacat tcggtctctt acaaacgttc attctatctt cctaatgttg atgaagaaaa
      901 aatcactgga acctttaaaa acggtgtgct gaaattggta ttacctaaaa cagcttatca
      961 gccaaaagaa acaaaaaaaa ttgagttaaa ttagaactta tcgcctctcg atgaatgagg
     1021 ggcgattttt atatatttgg ataagaacga gtatgtttaa attttcaaaa tgagcaaaaa
     1081 aatattttgt ttttacgata aagatgttat gatctctctt ggtaaaaaat atgggagttg
     1141 atcatttgat tgtcttaagt aaacttttgt tttggatacc atttatcata atgaccagtg
     1201 ctttctttta cttattcaaa aggcctaaca aatacttact cttatctgct tgtccgatga
     1261 tctattttat ccataagatt agaggatttt attattacga ctctggtatc tcattgtttt
     1321 gctacgaaat cggcttatta gcttcggcca tctttttcat atttgtgtta gaatatttca
     1381 gaaaaaaata aactt
//
LOCUS       DACELF010000197         1385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.215, whole genome shotgun sequence.
ACCESSION   DACELF010000197 DACELF010000000
VERSION     DACELF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.215"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1307
                     /locus_tag="IW687_002764"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="IW687_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0951977.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          595 a          103 c          233 g          454 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagt
//
LOCUS       DACELF010000198         1336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.216, whole genome shotgun sequence.
ACCESSION   DACELF010000198 DACELF010000000
VERSION     DACELF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.216"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1129)
                     /locus_tag="IW687_002765"
     CDS             complement(146..1129)
                     /locus_tag="IW687_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0951978.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DACELF010000199         1327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.217, whole genome shotgun sequence.
ACCESSION   DACELF010000199 DACELF010000000
VERSION     DACELF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.217"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..507)
                     /locus_tag="IW687_002766"
     CDS             complement(<1..507)
                     /locus_tag="IW687_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0951979.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNN"
     gene            complement(504..1157)
                     /locus_tag="IW687_002767"
     CDS             complement(504..1157)
                     /locus_tag="IW687_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0951980.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          429 a          211 c          120 g          567 t
ORIGIN      
        1 attatttttt attgattttg cataaattaa agaaataata gcagaaaata aaattgatat
       61 taataaagaa tatggtaaaa atttaaggct taaatctttc gcatcttttt gcatatcagc
      121 tgtagaaaca aattgtagat ttgttttttt accatcatta agctttattt ctctttcttc
      181 aattatcaaa gaattactat cactttctaa attaacattt atattatctt tgatttgtat
      241 ttcattctta ttattttttt cttttatgaa tacttttatt tcactactct tggaataaaa
      301 ctccaaagtt tgttctatat attttatttc ttttccattc atattactag aaatttcatt
      361 tgccatatta tgaatctttt cttttctcgt ctccaaatat gttttaggaa agataaaaaa
      421 aattaatgaa tgaactaata taattattat tccaagaaca gaaaatattt gtatgaacat
      481 ttttggaaat atctttaatt ttttcatttt ctctccaatt tatatcctac atttcttata
      541 gtagttatac agtctaattg taattttttt ctaagttctt ttatatatac atctattact
      601 cgatcataag gagtttcttc gctatctttc catacataat caataatttg cattcttgtt
      661 aacacttgtc cattattatc caataaacat tttaatactt ctagttcttt tgcatttaca
      721 tctatttctt caccatttat tttagctgta tagctattaa aattaaccga tagatcctta
      781 tattcaaact tctctaaatg tccataattc ttcttaatta aagaatctat tctagctttt
      841 aagaccggca atgaaaatgg cttttctaca tatccgtcta ctaaattagt aaatgcatca
      901 attttatatt cttcatcact aaatgcagtc aagattagaa ttggtaaatt gcttttcttt
      961 ctaatttctt tcaacacttc taaaccattt ataaaaggta tctgaatgtc taagataacc
     1021 aaatttatat cgctattaaa ttttgacaag gcttctcttc catctttagc ttgaataaca
     1081 gtatatccaa attctgaaag atattcactt attccctctc ttatcatatt atcatcttca
     1141 acagttaata ttttcatata tacctccact taagcctata cactagatat tatacagcat
     1201 attatggctc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     1261 ttttaataaa tgcaaacaga caccttgaaa tcctttagaa aaaaggcttc tataatattt
     1321 tgtgttg
//
LOCUS       DACELF010000200         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.218, whole genome shotgun sequence.
ACCESSION   DACELF010000200 DACELF010000000
VERSION     DACELF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.218"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..1248
                     /locus_tag="IW687_002768"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IW687_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACELF010000201         1299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.219, whole genome shotgun sequence.
ACCESSION   DACELF010000201 DACELF010000000
VERSION     DACELF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.219"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..357
                     /locus_tag="IW687_002769"
     CDS             <1..357
                     /locus_tag="IW687_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-transporting ATPase"
                     /protein_id="HAQ0951981.1"
                     /translation="LGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFNVRSSQSI
                     FKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGILSIVPIAV
                     NELIKFHRLPEAEEEE"
     gene            complement(525..1223)
                     /locus_tag="IW687_002770"
     CDS             complement(525..1223)
                     /locus_tag="IW687_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0951982.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT          384 a          268 c          242 g          405 t
ORIGIN      
        1 ttaggtagtt atgtcgatac tatcagccca tgggtagacg caagtcagca tgtcggtcaa
       61 actgttgcat tcttgatctt agcttattca tcgattctgc atgtgttcaa tgtacgcagc
      121 agccaatcga tcttcaaagt caatttagca accaataaag ctttggtaga aatggcgtta
      181 ctggcattag ctattacaac ggctgttgct ttgctgccat ttacgcaaga actattcggc
      241 ctagtacaca tcagtttgaa tcactggttc ttggtaggga ttttatctat cgtaccaatc
      301 gcggtgaatg aactgatcaa gttccatcga ttaccggaag cagaagaaga ggaatgatta
      361 actgtttcta agttgatttt ctttttactg gaactataca aaaaggaggt gtgacatgaa
      421 tgttgtcaca cctccttttt gctaatgggc tgaatgtttc ttttactgcc tctcgataaa
      481 cggtgttctc ctaccaaccc ctcggtcaca agtcacactc tcttttaaat atcttttggc
      541 aattgttcat aaaatgctgc ttttgttgcg gaaatgtatg ggctgagcca tagataacca
      601 ataccagcag ttgctaatgc taagatatgc cagccgataa atgaaagatc taaccagaaa
      661 agttgtcctt tataaccatc catcaatttt cgacttgcag tgatcgtatc cagtacttta
      721 ggtgtttgtc ctgtttcttg gacgatatcg tagtagataa aatagctttg agaataagaa
      781 tagcttttaa tgattccagg aataactaat agcaaacccc ataatgttgt aaagatcgag
      841 atcagcacag ctaagccgat cactccgccg ataaacaaac ctttgaatcc acgaaatgca
      901 tctttgaatg gttcgatctg atctttttgt ccacgtaaaa tatcaagata agtccaagaa
      961 ataccgctca tgaacaaagc gctaatgatg ctggaaataa ttccgccacc gcctcctgaa
     1021 ctaccgcctc ctgaagttgt cgcatctgag acgctcgctg atccatcata tatggtggtg
     1081 gcaaataaga taataggaat cataataaga agaatagcag caataccaat caatgtaggg
     1141 attagattta gtaaaacagc ttgtccccaa cgtcctttta gactctcttt tgcttggcgt
     1201 tttaattctg cagaagattg catgattttt ccctcctttt ctttaataac accaatatac
     1261 catgtatcaa aaaatatttc tgtcataatg tttttctct
//
LOCUS       DACELF010000202         1268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.220, whole genome shotgun sequence.
ACCESSION   DACELF010000202 DACELF010000000
VERSION     DACELF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.220"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..411
                     /gene="mgsA"
                     /locus_tag="IW687_002771"
     CDS             1..411
                     /gene="mgsA"
                     /locus_tag="IW687_002771"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0951983.1"
                     /translation="MIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEATGLP
                     IHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYEIPLA
                     TNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            734..>1268
                     /locus_tag="IW687_002772"
     CDS             734..>1268
                     /locus_tag="IW687_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0951984.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT          378 a          231 c          278 g          381 t
ORIGIN      
        1 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
       61 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
      121 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
      181 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
      241 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
      301 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
      361 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
      421 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
      481 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
      541 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
      601 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
      661 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
      721 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
      781 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
      841 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
      901 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
      961 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
     1021 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
     1081 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
     1141 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
     1201 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
     1261 cgctaaat
//
LOCUS       DACELF010000203         1259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.221, whole genome shotgun sequence.
ACCESSION   DACELF010000203 DACELF010000000
VERSION     DACELF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.221"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW687_002773"
     CDS             5..403
                     /locus_tag="IW687_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0951985.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW687_002774"
     CDS             387..1082
                     /locus_tag="IW687_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0951986.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          475 a          189 c          258 g          337 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataa
//
LOCUS       DACELF010000204         1247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.222, whole genome shotgun sequence.
ACCESSION   DACELF010000204 DACELF010000000
VERSION     DACELF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.222"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(69..1115)
                     /locus_tag="IW687_002775"
     CDS             complement(69..1115)
                     /locus_tag="IW687_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951987.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT          378 a          237 c          215 g          417 t
ORIGIN      
        1 agatttgtgc ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg
       61 tattcatttc aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc
      121 aataattctt tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca
      181 agtatttttt tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa
      241 atctgttcca gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt
      301 agttggaaat caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa
      361 aattttttca tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca
      421 aacagtttac cataatttga caggttttct gcctctaatt gataaaaata gatcatttct
      481 tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac
      541 agattttgca aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca
      601 atctgctttc cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc
      661 agattgatgg tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt
      721 aatgctcggc ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt
      781 gtatgttttt gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata
      841 aacatggatt gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt
      901 aaatgattgc attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat
      961 aacgggttga tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt
     1021 ggctgatcaa tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc
     1081 gtcaacgcat cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt
     1141 attttaccat tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg
     1201 gaaataaata aaaaggctct ataatttatg ggactgatga atcagaa
//
LOCUS       DACELF010000205         1236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.223, whole genome shotgun sequence.
ACCESSION   DACELF010000205 DACELF010000000
VERSION     DACELF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.223"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1236
                     /locus_tag="IW687_002776"
     CDS             <1..>1236
                     /locus_tag="IW687_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0951988.1"
                     /translation="TKEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKE
                     TVKIKDVKTDITEKTEVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESD
                     AENQSVSKEGTDLEAKIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQ
                     KKKSKKVPYLVLAAVLLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYT
                     DDHHQFIRADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIA
                     PAIVDDHLADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIV
                     FKDGKVVDSATRDQYKTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKK
                     AEEAKKAAEEQAKQAAQAAA"
BASE COUNT          578 a          204 c          239 g          215 t
ORIGIN      
        1 acaaaagaag agcacgcgaa agagctgctc aaaatgatca gtgaaaaaat tgcacaggaa
       61 aacaaagtag agccagaaaa ggaacaagaa actgaaaaag actctgatag agaaaacgcc
      121 acaggtaaag aaacagtaaa aataaaagac gtaaaaacgg atatcacaga aaaaactgaa
      181 gtatttactg aaaaacaatt agaaaaggaa aaaacgaata aactgccgga agcagagaaa
      241 aaagaaatac tcgaaaacac agacaaaaag tcaacagagc ccgatgaaat aaatagtgaa
      301 tccgatgctg aaaatcaatc tgtaagcaaa gagggtactg atcttgaagc aaaaatagaa
      361 aaagaagaga accagcctaa gctgtttgaa gcagaagcga aatcaataac aatcgtgcca
      421 gatactgaaa aaacacatca agaatcaaac ttagaaaaaa cagatccaga taaagcgcaa
      481 aagaaaaaat caaaaaaagt accttatctc gttttagcag cagtacttct actcggtgga
      541 ggaagctggg cttattatga ccatcatcaa aaagtagaag ccgaagtagc agcagaagca
      601 aaaagacagc aacaaatgga agaacttcat gagaaacttg ctgcctttta tacagatgat
      661 caccatcaat ttattcgtgc agatatgatt catcaagatt tatcaaaact aaaaaatgat
      721 ttatccagtc ttaagaatga aaaaggatat tcagagttag aaacaactta ccaagatatc
      781 caatctaaga tccgagatat ccaaaaagtg aatgagtggt tcatagcacc tgccatcgtc
      841 gatgatcatc tagcagatga catcaagttg aaagcagata aagagatcca agcaatcgaa
      901 acggataaca cagattttgg aaaattgatt caacaagcac aaacagaagc taaaaatcaa
      961 tatgctgaat tgcaaacagc taaagaaaaa acagctatag tctttaaaga cggaaaagta
     1021 gtggattcag caacacgcga ccaatacaag acggcaaaag aagcagtaga aaaagtgaaa
     1081 aatacagatc tatctgctca gttgaatgaa caattgaaac aagtagataa gatgctcact
     1141 gataaagaga aaaaagaaga agaggaaaag aaaaaagcag aagaagcgaa aaaagcagct
     1201 gaagaacaag caaaacaagc cgctcaagca gcagct
//
LOCUS       DACELF010000206         1230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.224, whole genome shotgun sequence.
ACCESSION   DACELF010000206 DACELF010000000
VERSION     DACELF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.224"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            29..1207
                     /locus_tag="IW687_002777"
     CDS             29..1207
                     /locus_tag="IW687_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0951989.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          452 a          229 c          205 g          344 t
ORIGIN      
        1 accaattcac agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac
       61 tctagtcact aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa
      121 cacactccta cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg
      181 ttttaattca ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg
      241 tgaattaaca ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc
      301 agcctacaaa agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg
      361 tgttacgatg tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc
      421 acaaaccatg tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag
      481 agccttaaat gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg
      541 tcaaaccgta tccaaagaag cgatttatat tgccattggt atacgagaag acggcactaa
      601 agaagtactg agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct
      661 acaggatatt aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa
      721 aggcatgaaa gatactatcc atcaaattta tcctaaagca aaatatcagc attgttgtat
      781 ccatgtatct cgtaacatcg ctcataaagt acgtgtcaaa gaccgaaaag aaatctgtga
      841 tgactttaag gctgtttatc aagctaactc aaaagaagaa gcgaatacct tcttatccgg
      901 catgattgag aaatggaaga aaaactatcc taaagtgacg cagtcactca tagaaaacca
      961 agacttatta actttttatg attttccacc tagcattcgt agaaccattt actcaaccaa
     1021 tctaatcgag tctttcaata agcaaattaa aagatacagc cgtagaaaag agcagtttca
     1081 aaatgaagaa tcactagaac gctttctagt cagcattttt gatacataca atcaaaaatt
     1141 tctaaacaga agccataaag gttttcaaca ggtaaccgat acattagttt caatgtttac
     1201 tgagtaacta attattttgc aggaggacaa
//
LOCUS       DACELF010000207         1230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.225, whole genome shotgun sequence.
ACCESSION   DACELF010000207 DACELF010000000
VERSION     DACELF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.225"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1104
                     /locus_tag="IW687_002778"
     CDS             <1..1104
                     /locus_tag="IW687_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0951990.1"
                     /translation="RYQLVAEQELDKNDLSYRVMQQVFDYYQLPLFEDEYHYIYSDTF
                     DTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNLYAHLSMRLSRKKKFVNEYNPF
                     KEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAYIALHFLVSYEREEDLRSVRAV
                     YICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQEMIATKDPDLVISIFPINGIDR
                     PFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLNLRETSINKDTLEDFSKELILK
                     AYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITFDQQYMAEGRQVDQIILDKGTL
                     VGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1104..>1230
                     /locus_tag="IW687_002779"
     CDS             1104..>1230
                     /locus_tag="IW687_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAQ0951991.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQ"
BASE COUNT          441 a          196 c          228 g          365 t
ORIGIN      
        1 cgttatcaat tggttgctga acaagaatta gacaaaaacg atctttctta tcgcgtcatg
       61 cagcaggtct ttgattatta tcagttgcca ctgttcgagg atgaatatca ttatatctat
      121 agtgatacat tcgacacgat tcaccagcaa gacgtcatga aactagttga gaatgtgatt
      181 tataaagtaa gtaaagcaat caaccattct ttttatcaag atccacaatt gttgacgaat
      241 ctgtatgctc atttatccat gcgattatcg agaaagaaaa aatttgtcaa tgaatataat
      301 ccttttaaag aagatatcaa gcagaaatac ccggaattat ttgatgcgat caagcaagca
      361 gttcgacaag aaataagtga acaaaatatt ctgatcaatg attctttcgt ggcatatatt
      421 gccttacact ttttggtttc ctacgaaaga gaagaagatc tacgaagcgt tagagccgtc
      481 tacatttgtt caacagggtt gggtgtgact agtctgatca agcaaaagat caatgaagaa
      541 ttaaataata tcactatcgt ggcatttgct tcagtattga atgcgcaaga aatgattgct
      601 accaaagatc ctgatttggt gattagtatt tttccaatta atggcatcga tcgtcctttc
      661 atcaaagtcc atccattacc cactgaacag gacttgaaaa aaattcaaca aataataaaa
      721 aaaatacttg cttcttccaa tcagcaggaa aggcataact tgaatcttag agaaacgtcc
      781 atcaataaag atactttaga agactttagc aaggaattga ttttgaaagc ttatacgatt
      841 tatgaacgtt tacttgtcat tttggaagca gaattgatcc aagaatatcg agaagctttt
      901 ttgcttcatg tattcttaat ggttcatcgt atcacatttg atcaacaata tatggcagaa
      961 ggtcgtcaag tagaccagat tattttagac aaaggaacac tggtaggaaa aatcgaagca
     1021 gtttttgcag aaaataattt aggaatcaat cagtcagaaa tcagtgcatt attctcatat
     1081 ataaaagaag gagcgtcaag ttagtgaaaa tgtcagacga ggctttaaaa attatcgagg
     1141 aaagtcaaaa tcgggaaaag ttaaaacaag tgattgatct aattgaagag cgattagtag
     1201 aacatgctat tgtaccaacc gagttgcagt
//
LOCUS       DACELF010000208         1230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.226, whole genome shotgun sequence.
ACCESSION   DACELF010000208 DACELF010000000
VERSION     DACELF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.226"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..330
                     /locus_tag="IW687_002780"
     CDS             <1..330
                     /locus_tag="IW687_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0951992.1"
                     /translation="SSTPSSSNSANQPIKSARASDIADASNPAWTWAPGVQSKVIATC
                     IQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFYIVTINCKTGW
                     FGGNGSN"
     gene            526..>1230
                     /locus_tag="IW687_002781"
     CDS             526..>1230
                     /locus_tag="IW687_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0951993.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSAD"
BASE COUNT          427 a          220 c          255 g          328 t
ORIGIN      
        1 tcctcgaccc cttcctcttc aaactcagcg aatcagccaa tcaaatctgc cagagcaagt
       61 gatattgccg atgcatctaa tccagcatgg acgtgggcac caggtgtcca aagcaaagtg
      121 attgctacat gtatccaacg tggatatatc gttgaaggag gttacagatt agaaaaagcc
      181 agaatcgaaa atggggaagg ctattataat ctatatgcca cttcaacaaa atctgcattg
      241 atgaaaggca ttggagaaag tgctttgcca ttttacatcg taaccatcaa ctgtaaaact
      301 ggatggttcg gaggaaatgg gagcaattaa ttttaggcaa atgaaagagg ctgtgagcct
      361 gccgtttaac accgtttatt cgaaaaaggg gagtatgaca caactgttgt catactcctt
      421 ttttcacctt tccaaaccaa ggctcagcta ttcaagtaga taacattatg ttatgatagc
      481 aaagaaatgt attaagaaaa cctttcacta tagagggaga aaaaaatgaa cgatttatta
      541 gcaactatat ttacaggtat tattattgat gaaaatgaaa aaaactactt tgtccaaaaa
      601 aacggacaaa cctttggctt aagcaaatca gaaggtgaac acaaaatcgg tgaagcagtt
      661 gaaggattcg gttaccaaaa tcaaaaacat gaaaatcgga tcactacaca aattcctaaa
      721 agccgtatcg gtcattacgc ttttggaatc gttactgcta cacgaaaaga tttaggtgcg
      781 tttgtcgata tcggtcttcc agataaagac tttgtcgtat ctcttgatga attgccgaca
      841 atgacagagc tatggcctaa aaaaggggat cgcttgatga tttcccttag agttgatgca
      901 aaagaccgta tttggggcag tttagcagat gaaaaaatat tcaaatcttt aagcaaatat
      961 ggaacagaag aaatgaaaaa caaagatgtg ataggaacag cataccgctt gaaattaaca
     1021 ggtacgtacg ttttaacgga agatttctat cttgggttta tccatccttc tgaacgatat
     1081 aatgaacctc gtctaggcga gcagataaaa ggtcgagtga ttggtgtccg accagacggt
     1141 gtactgaatc tttcattgaa gccaagagca tatgaagcta tatcagatga tgcacaaatg
     1201 attttaacca ttttagaacg ttctgcagat
//
LOCUS       DACELF010000209         1224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.227, whole genome shotgun sequence.
ACCESSION   DACELF010000209 DACELF010000000
VERSION     DACELF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.227"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..333)
                     /locus_tag="IW687_002782"
     CDS             complement(<1..333)
                     /locus_tag="IW687_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0951994.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
     gene            complement(463..>1224)
                     /locus_tag="IW687_002783"
     CDS             complement(463..>1224)
                     /locus_tag="IW687_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0951995.1"
                     /translation="GLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQD
                     ATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGS
                     LIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFL
                     AQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFM
                     IYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          392 a          226 c          173 g          433 t
ORIGIN      
        1 gatgtcggag acatctcgtt ttctattagg aatcaactca ggttcaaaag aagcctcccg
       61 gtctctggga acatcaatgt ttactttacc aaagctcgtt ttaagcgtct tattgccata
      121 accgtttcgt cgattgtcat gtttctttgg ctctttagaa tgggcgtcat accctaaatg
      181 gttattcaat tctccttgaa gcattttttc aaataaggga ccaaacacat cttttaaaac
      241 atcttgcata tcctctacag attcgggttg ataagtattc aaaatggatt cagctaactt
      301 ctcagcatca ggatttcttt tctttctagc catcgtgtaa tcacctcttg atcttatttt
      361 agaaaaataa aaaccgtgaa gcaaccaccc gcctaggatt gatggttact tacacggttt
      421 atattacact ctcatttttg gtgctaaaaa gcaccgaaaa ttttaattat tgtcaattaa
      481 taattctttt ggattccttc ttaatatatt gaatgaagaa ataatcaatg atacaagaag
      541 tactaaactc ataaatataa ctacataaat catgaattta ggtaatacat tgatatctaa
      601 acttgataat gtcttgttaa atccttcagc ttctgcccca ccacctaatt ggctagatgc
      661 agattgttta gctatttgtt tagctatatc accagtaacc ttattcaata tattattccc
      721 aagcttgtca gctgtatatt gagctaagaa ataggaacca acgaatgcgg ggattgatat
      781 aaataccatc tcaattagga attgaccaaa aatttctaat tttgatatac ctaatgatag
      841 taatactgct atttctttct ttctagcatt catccataag aataataata atgaaactac
      901 aactcctgca aatattaatg agccaaaaaa taatttattt gaaattgagt aaatacctga
      961 tatagattgt tgtaatgcag ggtaatttga tgaactttta atcaaattat attctctcca
     1021 atttatatct aatttcccaa gatcttttat tacactatca agattcttat caccttttac
     1081 aaagaatgtt gcatcttggt atacagctgt atcttcggta ttaccataaa ctttagcagc
     1141 tgtatgaata tcagtgatta atgtattttc gtatagttct tgtgctgcac ttactacacc
     1201 gctattatgc ccatcgaata gacc
//
LOCUS       DACELF010000210         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.228, whole genome shotgun sequence.
ACCESSION   DACELF010000210 DACELF010000000
VERSION     DACELF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.228"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW687_002784"
     CDS             414..947
                     /locus_tag="IW687_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0951996.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACELF010000211         1206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.229, whole genome shotgun sequence.
ACCESSION   DACELF010000211 DACELF010000000
VERSION     DACELF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.229"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..1072
                     /locus_tag="IW687_002785"
     CDS             149..1072
                     /locus_tag="IW687_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0951997.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
BASE COUNT          446 a          191 c          240 g          329 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgca
//
LOCUS       DACELF010000212         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.230, whole genome shotgun sequence.
ACCESSION   DACELF010000212 DACELF010000000
VERSION     DACELF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.230"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW687_002786"
     CDS             <1..1076
                     /locus_tag="IW687_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0951998.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACELF010000213         1180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.231, whole genome shotgun sequence.
ACCESSION   DACELF010000213 DACELF010000000
VERSION     DACELF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.231"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(298..720)
                     /locus_tag="IW687_002787"
     CDS             complement(298..720)
                     /locus_tag="IW687_002787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0951999.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(734..>1180)
                     /locus_tag="IW687_002788"
     CDS             complement(734..>1180)
                     /locus_tag="IW687_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0952000.1"
                     /translation="HLDATTNLERKLTEQGIYPAVDPLASSSSALAPEIVGEEHYKVA
                     TEVQHVLQRYRELQDIIAILGMDELSDQEKVLVSRARRVQFFLSQNFNVAEQFTGLPG
                     SYVPVEETVKGFREILEGKYDDLPEEAFRSVGRIEDVVEKAKTLGY"
BASE COUNT          317 a          269 c          219 g          375 t
ORIGIN      
        1 agtcccttat tccaaataaa cggtgttcaa tcatctgacc ctcggtatta gctcaaaaaa
       61 cggcaaaata tgaggaacta ttttccattt gtttctctaa tactcaggac aaaacgctga
      121 tttcacaacc tccgatatac ggtgttccag aagtagcttc ttcgaaaaat aagctgagat
      181 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatgtcttga agctgaccac
      241 ttctgtcata acctcttttt actgttctta aacacggata aacatccgca tctcatttta
      301 tgagtgctta gatactttga tacgattgat tgctcggtgc aacgcaacag ttgcccgttt
      361 caattcgttc gtatcttgtt ttgcttttgc ttcttcgatt tggcgttctg cgcgttgttt
      421 tgcacgttcc gcacgagata catcgatatc acgttcacgt tccgcactat cagcaacgat
      481 tgaaacaaca ttatcacgaa cttccatgat tccgccatta acagcgatcc aatcgacgtg
      541 ggtatctgaa tctgttcgtt ttacacgaac ttcatcaatc gtcaatggaa cgatgattgg
      601 tgcatgttta ggcaaaatcc caagctcacc agctgtcgta cgtgcaacga cgatagctgc
      661 atggtgatca tagaccaaac cggcaggagt taccacattt actgttaaat attgatccat
      721 ggggcacccc tttctagtag cctagtgttt tcgctttttc tacgacgtct tcgattcggc
      781 caacacttcg gaaagcttct tcaggaagat catcgtattt gccttccaag atttcgcgga
      841 atcctttcac tgtttcttct acagggacat atgaacctgg aagaccagta aattgttcag
      901 ccacgttaaa gttttgtgac aagaagaatt ggactctgcg ggcacgagaa actaacactt
      961 tttcttgatc ggataattca tccataccta agatagcaat gatatcttgt aattcacgat
     1021 atcgttgcaa tacgtgttgg acttctgttg ctactttata atgttcttct cccacgattt
     1081 caggtgctaa agcacttgaa gaagaagcca atggatctac cgctggataa atcccttgtt
     1141 ctgttaattt acgttccaag ttggttgtcg catccaaatg
//
LOCUS       DACELF010000214         1138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.232, whole genome shotgun sequence.
ACCESSION   DACELF010000214 DACELF010000000
VERSION     DACELF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.232"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1138)
                     /locus_tag="IW687_002789"
     rRNA            complement(<1..>1138)
                     /locus_tag="IW687_002789"
                     /product="16S ribosomal RNA"
BASE COUNT          241 a          350 c          254 g          293 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgtt
//
LOCUS       DACELF010000215         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.233, whole genome shotgun sequence.
ACCESSION   DACELF010000215 DACELF010000000
VERSION     DACELF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.233"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            58..351
                     /locus_tag="IW687_002790"
     CDS             58..351
                     /locus_tag="IW687_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952001.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            348..572
                     /locus_tag="IW687_002791"
     CDS             348..572
                     /locus_tag="IW687_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0952002.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            569..>1137
                     /locus_tag="IW687_002792"
     CDS             569..>1137
                     /locus_tag="IW687_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0952003.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          389 a          175 c          240 g          333 t
ORIGIN      
        1 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
       61 attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt
      121 ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa
      181 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
      241 atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg
      301 gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa
      361 cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg
      421 ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg
      481 gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct
      541 cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag
      601 cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga
      661 gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt
      721 gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta
      781 taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt
      841 gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca
      901 gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat
      961 tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac
     1021 ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa
     1081 ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgc
//
LOCUS       DACELF010000216         1136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.234, whole genome shotgun sequence.
ACCESSION   DACELF010000216 DACELF010000000
VERSION     DACELF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.234"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..751
                     /locus_tag="IW687_002793"
     CDS             68..751
                     /locus_tag="IW687_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0952004.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..>1136
                     /locus_tag="IW687_002794"
     CDS             753..>1136
                     /locus_tag="IW687_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0952005.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEA"
BASE COUNT          380 a          241 c          205 g          310 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagct
//
LOCUS       DACELF010000217         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.235, whole genome shotgun sequence.
ACCESSION   DACELF010000217 DACELF010000000
VERSION     DACELF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.235"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..>1134
                     /locus_tag="IW687_002795"
     CDS             join(55..331,331..>1134)
                     /locus_tag="IW687_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0952006.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DACELF010000218         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.237, whole genome shotgun sequence.
ACCESSION   DACELF010000218 DACELF010000000
VERSION     DACELF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.237"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..280)
                     /locus_tag="IW687_002796"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IW687_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IW687_002797"
     CDS             complement(546..740)
                     /locus_tag="IW687_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952007.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          369 a          184 c          155 g          354 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc cc
//
LOCUS       DACELF010000219         1048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.238, whole genome shotgun sequence.
ACCESSION   DACELF010000219 DACELF010000000
VERSION     DACELF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.238"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW687_002798"
     CDS             complement(<1..132)
                     /locus_tag="IW687_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0952008.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..>1048
                     /locus_tag="IW687_002799"
     CDS             542..>1048
                     /locus_tag="IW687_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAQ0952009.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQW"
BASE COUNT          421 a          176 c          214 g          237 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggacgagtga cgcaatgg
//
LOCUS       DACELF010000220         1023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.239, whole genome shotgun sequence.
ACCESSION   DACELF010000220 DACELF010000000
VERSION     DACELF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.239"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..980
                     /locus_tag="IW687_002800"
                     /pseudo
     CDS             1..980
                     /locus_tag="IW687_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          334 a          189 c          235 g          265 t
ORIGIN      
        1 ccagaacaag cagaattgat cgcactaaga aagaaaaata agcaactcga aatggagaat
       61 gatattttaa agcaagcggc gctgatattc ggacgaaaag acaagtaatt gatgccaaca
      121 agcataaata ttccatatca gcgatgtgca aaattctaaa tatttctcgt cagacctact
      181 attatcaagc gaaaccgatc gaaaatgagt ccgacttaga agagatcgtt caggaagagt
      241 ttattcgaaa ccgaaaggct tacggtaccc gaaaattgaa gaagtgttta gcaaagcgtg
      301 ggcttcaact cagtcggcgc cgaatcggtc gaatcatgaa acgccgcgga ttgacatcta
      361 cctatacgat cgctcatttt aaagggcaac gaacagcttg taatgaagcg aaaacagcga
      421 atgtattaga tcggaccttt acacaagaac agccattgga agccatcgtt acggatctta
      481 cttatgttcg cgtggggaaa aagtggcatt atatctgctt aatacttgat ttgtttaatc
      541 gagaaattat tggttattcc tgtggtgaga agaaagatgc ctcattggta aaagaagcct
      601 ttggacggat accgtattct ttaacagacg tcaagctttt tcatacagac cggggaaagg
      661 aatttgataa ccaaaccatt catgagattc tgaatggttt tggaattact cgttcattga
      721 gtaggaaggg ttgtccgtat gataatgccg ttgtggaatc aacctataaa tctgtcaaag
      781 tagaattcgt gcatcaatac caatttgaga cactggcaca gctacgtcta gaattgtttg
      841 attatgtgca ttggtggaac tatctacgct tacatggcac gttggcgtat gaaacaccga
      901 tccaaattcg acaacagaga ttggcgaagc gaatccttga taatgagcgc ggatctgata
      961 cctctggaga ggcagcgtaa ttgaatgatt gtgcttctgc cggagaaaat cagatccgag
     1021 gat
//
LOCUS       DACELF010000221         1021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.240, whole genome shotgun sequence.
ACCESSION   DACELF010000221 DACELF010000000
VERSION     DACELF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.240"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1021
                     /locus_tag="IW687_002801"
     CDS             <1..>1021
                     /locus_tag="IW687_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952010.1"
                     /translation="KDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKNFAK
                     EAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSIPVD
                     DQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPVDVV
                     TALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKDSFE
                     NPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSSELA
                     AQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDRRQV
                     LNALAD"
BASE COUNT          336 a          184 c          222 g          279 t
ORIGIN      
        1 aaaggataaa gaattattga cacaattgac ttcagaaggc gctaaaggac cattcgtcac
       61 gatcatgctg aatacgcatg tcgcccatca agatgtggaa aaagatcaga tcaaattcaa
      121 aaattttgcg aaagaagcaa agaaacgttt tgagaaaaaa tattccgaac atgactggtc
      181 cccgttccaa gaaaaaattg aaaatttgtt aggagatcct tcgttttggc gtagtgcaac
      241 tacgagtgta gcggtcatta taactgctga ccagacctat atccatcgtt tgagtatccc
      301 ggtagacgat caatactatg taagtgatct tccttattta ctggctatta tcaaaaatgg
      361 gcagttcaac tattcttatt atttattggg attgaaccgc gactccatga aattgtatga
      421 ggtcagcaac aaacaagtaa aagaagtatc tctaccagaa ggggcaccgg ttgatgtcgt
      481 tactgcattg ggatctgaac tgacaggtgg cggtttgaac ttctcttcta atggaggagg
      541 cggagcccga gatggagtaa ctttccatgg cgtaaatcca aaagatgaag aagtagagat
      601 cgattgggtc aactattacc aagctgttga tacgtacttg aaggattctt tcgaaaatcc
      661 tgaacaatgg ccgatttatt tatttgcatt accagaaaat cagacgatgt tcaaaaaaat
      721 tgctaaaaat ccctattatt gtgcagacgt tgcgatttct gcttctcctg cgcagctatc
      781 ctttagagat atcgaagaag gggcgaagaa gctgtcaagt gaactcgcag ctcaagaatc
      841 aaaagaatac aataaactct tagataagaa attcatcgat cagttagttg atatcgttcc
      901 agctgcgaaa gaaggaaaag tgtcacattt atttatcgct acgtcaaact tagctgatgg
      961 tttcggtgaa gatcctgaaa cagaatatga tcggcgtcaa gtgttgaatg cattagcaga
     1021 t
//
LOCUS       DACELF010000222         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.241, whole genome shotgun sequence.
ACCESSION   DACELF010000222 DACELF010000000
VERSION     DACELF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.241"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW687_002802"
     CDS             complement(45..779)
                     /locus_tag="IW687_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0952011.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          352 a          209 c          181 g          260 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aa
//
LOCUS       DACELF010000223          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.242, whole genome shotgun sequence.
ACCESSION   DACELF010000223 DACELF010000000
VERSION     DACELF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.242"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..211
                     /locus_tag="IW687_002803"
     CDS             <1..211
                     /locus_tag="IW687_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952012.1"
                     /translation="KNVYVQTTDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFL
                     HSTAAAFAFTHTQKYDGHHPVIYEV"
     gene            663..863
                     /locus_tag="IW687_002804"
     CDS             663..863
                     /locus_tag="IW687_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0952013.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          340 a          167 c          184 g          307 t
ORIGIN      
        1 caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa cagaagaacg
       61 gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt atgaaagttg
      121 ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc agaaatatga
      181 tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa actaatctcg
      241 ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca gaaataccat
      301 gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac agaaccgata
      361 agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa aaaaacacca
      421 actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat gtaatcgctg
      481 agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa acggtatgat
      541 atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa taaacatctt
      601 ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg aggattttta
      661 ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga ttcatcactg
      721 cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac ggattcaaaa
      781 ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc ggacctcaag
      841 caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc agacatttgc
      901 gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt tctgcacttt
      961 gtagtataat ttcaaaagaa cattagaggc aggagaag
//
LOCUS       DACELF010000224          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.243, whole genome shotgun sequence.
ACCESSION   DACELF010000224 DACELF010000000
VERSION     DACELF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.243"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            281..972
                     /locus_tag="IW687_002805"
                     /pseudo
     CDS             281..972
                     /locus_tag="IW687_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          428 a          141 c          166 g          263 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaatc agttaacaga tacacaaaag
      841 aaattagacg aagcaactgc tgaaaactca agcttgaaac aatatattca aaagttagaa
      901 aaagcaaaag atgaagtgga agatacagct gataaatctc agcaaattgt tgaagaacat
      961 ttgcgtaaat aatctttctt aacaagtaaa cctaaatt
//
LOCUS       DACELF010000225          990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.244, whole genome shotgun sequence.
ACCESSION   DACELF010000225 DACELF010000000
VERSION     DACELF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.244"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            166..639
                     /locus_tag="IW687_002806"
     CDS             166..639
                     /locus_tag="IW687_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0952014.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            648..875
                     /locus_tag="IW687_002807"
     CDS             648..875
                     /locus_tag="IW687_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0952015.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          334 a          148 c          163 g          345 t
ORIGIN      
        1 taaatgtttc ttacaatcaa aaaaagctgt taaatgcact ccacaccttc atactctatc
       61 attttttata aacgatatat ttttattgtt tttcgataaa aaatagttgt tttacaataa
      121 ttattctgct atattttagc tatcaaagat aaaagaggga taaaaatgaa atttaaaaca
      181 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
      241 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      301 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      361 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      421 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      481 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
      541 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
      601 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
      661 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
      721 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
      781 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
      841 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
      901 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca
      961 gattttttat ttcgattgaa taccgtttta
//
LOCUS       DACELF010000226          954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.245, whole genome shotgun sequence.
ACCESSION   DACELF010000226 DACELF010000000
VERSION     DACELF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.245"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..95)
                     /locus_tag="IW687_002808"
     tRNA            complement(25..95)
                     /locus_tag="IW687_002808"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Cys,seq:gca)
     gene            complement(141..212)
                     /locus_tag="IW687_002809"
     tRNA            complement(141..212)
                     /locus_tag="IW687_002809"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Gln,seq:ttg)
     gene            complement(224..296)
                     /locus_tag="IW687_002810"
     tRNA            complement(224..296)
                     /locus_tag="IW687_002810"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:His,seq:gtg)
     gene            complement(299..372)
                     /locus_tag="IW687_002811"
     tRNA            complement(299..372)
                     /locus_tag="IW687_002811"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Trp,seq:cca)
     gene            complement(379..459)
                     /locus_tag="IW687_002812"
     tRNA            complement(379..459)
                     /locus_tag="IW687_002812"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:Tyr,seq:gta)
     gene            complement(461..536)
                     /locus_tag="IW687_002813"
     tRNA            complement(461..536)
                     /locus_tag="IW687_002813"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Phe,seq:gaa)
     gene            complement(572..644)
                     /locus_tag="IW687_002814"
     tRNA            complement(572..644)
                     /locus_tag="IW687_002814"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Asp,seq:gtc)
     gene            complement(654..726)
                     /locus_tag="IW687_002815"
     tRNA            complement(654..726)
                     /locus_tag="IW687_002815"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Val,seq:tac)
     gene            complement(742..813)
                     /locus_tag="IW687_002816"
     tRNA            complement(742..813)
                     /locus_tag="IW687_002816"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(778..780),aa:Glu,seq:ttc)
     gene            complement(828..917)
                     /locus_tag="IW687_002817"
     tRNA            complement(828..917)
                     /locus_tag="IW687_002817"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Ser,seq:gga)
BASE COUNT          269 a          247 c          218 g          220 t
ORIGIN      
        1 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
       61 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
      121 taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
      181 agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat gatgggaatc
      241 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatg
      301 gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt tctaccttta
      361 aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct aaggaacgga
      421 tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg ggacagaatc
      481 gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac cgcgccatat
      541 tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga acccgcgatc
      601 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca ttatggaggt
      661 taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca gctgagctaa
      721 acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta ccgccgtgaa
      781 agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg
      841 attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg ctccttcggc
      901 ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgag
//
LOCUS       DACELF010000227          908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.246, whole genome shotgun sequence.
ACCESSION   DACELF010000227 DACELF010000000
VERSION     DACELF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.246"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>908
                     /locus_tag="IW687_002818"
     CDS             82..>908
                     /locus_tag="IW687_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0952016.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQK"
BASE COUNT          303 a          168 c          180 g          257 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaag
//
LOCUS       DACELF010000228          907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.247, whole genome shotgun sequence.
ACCESSION   DACELF010000228 DACELF010000000
VERSION     DACELF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.247"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..808
                     /locus_tag="IW687_002819"
     CDS             <1..808
                     /locus_tag="IW687_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ0952017.1"
                     /translation="HDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGL
                     RTGTDNQGNEEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQ
                     PAIEQPNESENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLSVLLFFLLLFRK
                     SLRWTSYLIYLHFFASTNTIAYQKLLNPLETLVPRKPAESLQEYSLRIEQEFKLDLRF
                     IRLTTQYEAEIYGRQADPVKFDKKTYLEITHIIRKTVYKKILTKGSRFKK"
BASE COUNT          297 a          191 c          161 g          258 t
ORIGIN      
        1 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
       61 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
      121 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
      181 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
      241 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
      301 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
      361 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
      421 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
      481 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
      541 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
      601 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
      661 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
      721 taaaaaaacc tatttagaaa taacgcatat aataagaaaa acggtataca aaaaaattct
      781 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctcgaat
      841 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
      901 tcacaac
//
LOCUS       DACELF010000229          904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.248, whole genome shotgun sequence.
ACCESSION   DACELF010000229 DACELF010000000
VERSION     DACELF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.248"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..650)
                     /locus_tag="IW687_002820"
     CDS             complement(<1..650)
                     /locus_tag="IW687_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0952018.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAF"
     gene            626..799
                     /locus_tag="IW687_002821"
     CDS             626..799
                     /locus_tag="IW687_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0952019.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
BASE COUNT          280 a          150 c          185 g          289 t
ORIGIN      
        1 aaaaaagcca gctttagaat atgataatcg tcatagtttc taggggtata accagtgaga
       61 gaatggaact tgttcaatcg atattgcaag gtgtttttat gaataaataa ttcttctgag
      121 caatttttga tgcttccgtt atttttactg aagcatgcca aagtcttctt gaacaaatgt
      181 acttcttctt ctgtcaggct tttcaaaata cgttttacat ataatgttcg ttgatctgac
      241 gggattgctg gtaaaacgat cccgaggtct aaatcttcaa aaaagcggat ctcgttcaca
      301 gattgatgga aagctatcca ttcgcaaact tgtttggcat gaaggaaaga ttgcggtgtc
      361 tcttgctctg tataacaaag cgacccaatt ccaaagttta gctgtaactg atacatttta
      421 ttaaactttg tctggatttt atcaagcaat gatagcaatg aggctctgga aatccgattg
      481 actaggataa tgatttgttg tctatgtaca gtgagcaaag cctgtccgta ataagagaag
      541 accctttcga gcaactccaa taaaggattg atccgttctt gacacaattg ctgattgtca
      601 gaaagttgtc ccacaactac ataatatgga gaagaaaaat caagctcaat taagtgaggg
      661 tggtatcgac tatgtacttg atatcatcaa tgaaccgatg gtactttctt atgcgatgga
      721 acaggtagag cgattgcttg tttgtgcagg aaaacaagcg atgcggctga tacgattagg
      781 tgagaaaata agaagataaa aatacacggt cccttcatgg cgaccgtgca tttttggttt
      841 ttattaaata tttaatactt tattcaagaa atcttttgtc cgttcatttt gcggattttc
      901 aaag
//
LOCUS       DACELF010000230          901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.249, whole genome shotgun sequence.
ACCESSION   DACELF010000230 DACELF010000000
VERSION     DACELF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.249"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW687_002822"
     CDS             complement(<1..563)
                     /locus_tag="IW687_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0952020.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
     gene            complement(574..780)
                     /locus_tag="IW687_002823"
     CDS             complement(574..780)
                     /locus_tag="IW687_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0952021.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          247 a          201 c          145 g          308 t
ORIGIN      
        1 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
       61 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      121 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      181 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      241 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      301 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      361 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      421 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      481 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      541 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      601 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      661 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      721 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      781 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      841 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      901 c
//
LOCUS       DACELF010000231          897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.250, whole genome shotgun sequence.
ACCESSION   DACELF010000231 DACELF010000000
VERSION     DACELF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.250"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            86..>897
                     /gene="gndA"
                     /locus_tag="IW687_002824"
     CDS             86..>897
                     /gene="gndA"
                     /locus_tag="IW687_002824"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0952022.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQ"
BASE COUNT          309 a          155 c          209 g          224 t
ORIGIN      
        1 atgttgttct ttagtttgca atgtagccgt gttaagctat aatgtaagag attagggaga
       61 aatcccgtat gtaggaggag ttcatatgtc aaaacaacaa atcggcgttg tcggtatggc
      121 cgttatgggt aaaaatctag ccttgaacat agaaagccgc ggctattcag tggctttatt
      181 caatcgtaca ggggctaaaa caaccgctgt agtagaagaa cacccagata aaaatttcaa
      241 agcaacatat acaatcgaag aatttgtaga atctatcgag aaaccacgtc ggatcttgct
      301 aatggtcaaa gcaggaccgg caacagatgc aacaatccaa gagttacttc ctcaccttga
      361 taaaggcgat attttgattg acggaggaaa tacattcttt aaagatacga tgcgtcgtaa
      421 tgaagagtta gccaactctg gcatcaactt tatcggtaca ggcgtttccg gtggtgaaga
      481 aggagcttta aaaggacctt caatcatgcc tggtggacaa aaagaagcct atgcgctagt
      541 tgcaccaatc ttagagcaaa tttctgcaaa agcagaagac ggagcaccat gtgtcacata
      601 tattggccca aatggagccg gacattatgt gaaaatggta cacaacggta ttgagtatgg
      661 agatatgcaa ctgattgctg aatcttatga cttaatgaag aatattttaa acctttcagt
      721 agaagaaatg gctgatatct ttaaagaatg gaatcaaggc gaattagaca gctatttgat
      781 agaaatcact gctgacattc tgacacgtaa agacgatgaa ggaacaggta aaccagtagt
      841 agatgtgatc cttgatgcag ctgggaataa aggaacaggt aaatggacaa gccaaag
//
LOCUS       DACELF010000232          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.251, whole genome shotgun sequence.
ACCESSION   DACELF010000232 DACELF010000000
VERSION     DACELF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.251"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..888
                     /locus_tag="IW687_002825"
     CDS             <1..888
                     /locus_tag="IW687_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0952023.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACELF010000233          885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.252, whole genome shotgun sequence.
ACCESSION   DACELF010000233 DACELF010000000
VERSION     DACELF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.252"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..502
                     /locus_tag="IW687_002826"
     CDS             <1..502
                     /locus_tag="IW687_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0952024.1"
                     /translation="KEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNE
                     IDESLAEILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEM
                     SQNVQRLTWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAM
                     AAEGLV"
     gene            614..>885
                     /gene="pgi"
                     /locus_tag="IW687_002827"
     CDS             614..>885
                     /gene="pgi"
                     /locus_tag="IW687_002827"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0952025.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAA"
BASE COUNT          304 a          155 c          208 g          218 t
ORIGIN      
        1 caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta
       61 tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc
      121 tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat
      181 cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc
      241 aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt
      301 agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct
      361 ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga
      421 cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat
      481 ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt
      541 ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata
      601 aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa
      661 cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga
      721 aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa
      781 agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt
      841 agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagc
//
LOCUS       DACELF010000234          884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.253, whole genome shotgun sequence.
ACCESSION   DACELF010000234 DACELF010000000
VERSION     DACELF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.253"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..332
                     /locus_tag="IW687_002828"
     CDS             <1..332
                     /locus_tag="IW687_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0952026.1"
                     /translation="ESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILA
                     DEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVN
                     KMNLEVE"
     gene            332..>884
                     /locus_tag="IW687_002829"
     CDS             332..>884
                     /locus_tag="IW687_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952027.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIK"
BASE COUNT          383 a           91 c          158 g          252 t
ORIGIN      
        1 atgaaagtat cttgtttgaa ctaggtttag ataaaaaaca aataaaaaga aatgttatga
       61 aattatctgg tggacagcaa caaagagtag ctattgctag agcattggta tcagatgctc
      121 caataatact agctgatgag cctactggta atctagacag tgttactgct ggagaaataa
      181 ttaatatatt aaagacatta gctaaagata gaaataaatg tgtaatagtt gtaacacata
      241 gtaaggaagt agcagattct gcggatatca ttttagaact aagtggtaaa aagttgaaaa
      301 aagtaaataa aatgaatttg gaggttgaat aatgataaaa aatgcatttg cttatgtaac
      361 tagaaaaagt ttaaaatcat taattatcat attagttatt ttatctatgt caactttaag
      421 tctcattagt ttatctatta aagatgctac ggacagagct tcaaaagaaa catttgctaa
      481 tataacaaat agtttttcta tggagataaa tagacaagta aatccaggaa cacctagagg
      541 tgggggaaat gtaaaaggtg aagatattaa aaagatatct caaacagata gtatagactc
      601 ctatgtaaaa agaataaaca gtgttgcaga tttagttgat tatgatatta ttgaaactca
      661 agagactctt gcgaatcaat cacctgaaag agcaaagaat tttaaaagaa cagttatgtt
      721 aactggggtt aacgactcaa caaaagaaac aaaatttgta tcagaagcat ataaattagt
      781 agaaggaaag catttagaaa atgaagataa aaataaaatc ttaatgcata aagatttagc
      841 tgaaaaaaat aaccttaaag ttggagataa gataaaaata aaat
//
LOCUS       DACELF010000235          880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.255, whole genome shotgun sequence.
ACCESSION   DACELF010000235 DACELF010000000
VERSION     DACELF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.255"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..848
                     /locus_tag="IW687_002830"
     CDS             147..848
                     /locus_tag="IW687_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0952028.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          318 a          154 c          146 g          262 t
ORIGIN      
        1 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
       61 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
      121 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
      181 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
      241 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
      301 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
      361 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
      421 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
      481 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
      541 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
      601 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
      661 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
      721 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc
      781 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
      841 gagaataacg cgaacaaaaa taagcagtga ccctgataaa
//
LOCUS       DACELF010000236          865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.256, whole genome shotgun sequence.
ACCESSION   DACELF010000236 DACELF010000000
VERSION     DACELF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.256"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..865
                     /locus_tag="IW687_002831"
                     /pseudo
     CDS             116..865
                     /locus_tag="IW687_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
BASE COUNT          319 a          105 c          146 g          295 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagat
//
LOCUS       DACELF010000237          862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.257, whole genome shotgun sequence.
ACCESSION   DACELF010000237 DACELF010000000
VERSION     DACELF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.257"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          295 a          159 c          115 g          293 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gt
//
LOCUS       DACELF010000238          847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.258, whole genome shotgun sequence.
ACCESSION   DACELF010000238 DACELF010000000
VERSION     DACELF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.258"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..>847
                     /locus_tag="IW687_002832"
     CDS             206..>847
                     /locus_tag="IW687_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0952029.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIR"
BASE COUNT          309 a          107 c          175 g          256 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcga
//
LOCUS       DACELF010000239          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.259, whole genome shotgun sequence.
ACCESSION   DACELF010000239 DACELF010000000
VERSION     DACELF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.259"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..522
                     /locus_tag="IW687_002833"
     CDS             <1..522
                     /locus_tag="IW687_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0952030.1"
                     /translation="VPDDFITQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMY
                     NKKLEQETKRKIVPPEIKTWWRLELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKH
                     TDRIMIAGLISDQNLWAGLARHTKYKYRNLLKKESQSDELVNHLRETFSESADVLKKE
                     LDTWLLGLDVTEE"
BASE COUNT          270 a          133 c          212 g          231 t
ORIGIN      
        1 gtgcctgatg attttattac gcaatatcgt gtggttgacc ctgtttcatt caaaccaatc
       61 tatgggcggt ctgggaagct ggaaacggca tattggggta gtcgtgccag tgagcgacaa
      121 ataaggatgt acaacaaaaa attggaacaa gaaaccaaac ggaaaattgt accaccggaa
      181 ataaaaactt ggtggcgact ggaattgcag ttacggcgtg gaaaagctac tgattggtat
      241 gcgatggttc atgaaagttt agacagtttc gcaagtcctc attttttgcc gactgataca
      301 aagcatactg atagaattat gattgcagga ttgatttcag atcaaaattt atgggctggc
      361 ttggctcgtc atacaaaata taaatatcga aatttgttga aaaaagaaag ccaaagtgat
      421 gagttagtta atcatttacg agagacattt tcggaatcgg cagatgtttt gaaaaaagaa
      481 ttggatacgt ggcttttggg gttggacgtg acggaagaat gagtggatga atttttgtcc
      541 acttttttat tttcagcaca agtcggcatg gtgggtcgtg cgtttgcagc aagagcgaaa
      601 aaacgctaac gaatccgcaa atgccgactt ggataaaaca gttgcgcaaa gagacttggc
      661 tttttataat aaatttaggg atatcccagt gtcactcttt ggggtaattc gggggaagga
      721 gttacccctc actctggaaa attagagcga agcagacgag attatcccaa tgaatatgac
      781 ggcttactcg gagtgatacc gagtaaaaaa gtcagataag tgagagtaaa tttacgtcaa
      841 tgttta
//
LOCUS       DACELF010000240          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.260, whole genome shotgun sequence.
ACCESSION   DACELF010000240 DACELF010000000
VERSION     DACELF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.260"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..244
                     /locus_tag="IW687_002834"
     CDS             5..244
                     /locus_tag="IW687_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952031.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            311..550
                     /locus_tag="IW687_002835"
     CDS             311..550
                     /locus_tag="IW687_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952032.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            677..>846
                     /locus_tag="IW687_002836"
     CDS             677..>846
                     /locus_tag="IW687_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0952033.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQ"
BASE COUNT          343 a          101 c          166 g          236 t
ORIGIN      
        1 ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa aaggaaggta
       61 tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaatccc tgtctaaagg
      121 aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa cccataatgg
      181 aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga taacccaaga
      241 ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa caagaaaggt
      301 aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga aagaaatgga
      361 agaaaagttg ctaaaaatga tgaaacaaaa acaccttaaa agattatctg tcatgcaata
      421 tatcaatgat atgaagataa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa
      481 ctttgaacag ttgaggcgaa cttatgtaaa aacaggttcc aattgccaac tattattaga
      541 agtttcttga cgattatgat atgaaaatga tcaattatga tgtggcattg ttattgaata
      601 aaaagaatag taaaatttaa catggtttat cttggttaga tgtaggtaaa ttaattcata
      661 tatagaaggt agatagatga ataaaaacaa taaaacttta tttaatcctt acgtgggcgt
      721 aatcattgtt tttcttcttt ttagttattt tgcttatcaa ggttatcaaa aggacaaaat
      781 cgctgaaaaa gtagatggtg taacaaaaac aatccatatt caggaacagg ctaaaaccta
      841 tcaaga
//
LOCUS       DACELF010000241          842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.261, whole genome shotgun sequence.
ACCESSION   DACELF010000241 DACELF010000000
VERSION     DACELF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.261"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..230
                     /locus_tag="IW687_002837"
     CDS             33..230
                     /locus_tag="IW687_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952034.1"
                     /translation="MEDSLLINLYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDAR
                     SDHRRYDYIKYTFVDPERYKD"
     gene            complement(266..637)
                     /locus_tag="IW687_002838"
     CDS             complement(266..637)
                     /locus_tag="IW687_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952035.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(639..>842)
                     /locus_tag="IW687_002839"
     CDS             complement(639..>842)
                     /locus_tag="IW687_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952036.1"
                     /translation="IFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSELFSSDYDVL
                     DTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          279 a          141 c          111 g          311 t
ORIGIN      
        1 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
       61 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
      121 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      181 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
      241 aacaaacaaa ctcgcttgaa attcattagg tgagtttgtt tattattttt tctgcttctc
      301 gattcatcag cgccatatct gttttcgtaa tggtttcatt aggaatatag tcagcacatt
      361 ttcttttctc taacattcta tacccactat tatgtattcc taaggtattc aattccttaa
      421 ctgttgaaaa caaattccaa acggtttggt ggcttccttt tccgctactt ccattataaa
      481 taatatcata attttctttc aaatactcat cagatttttt aaaagcactg tagtatgctc
      541 ttgaaacagc agttcttcta taagattctt tagaattata tttatcatct gattcaattt
      601 gtttggaaat agataaatat tcattaaagt caaacatttt attcccccaa agttgctact
      661 acagatttcc ccatatattt atagtcagat aatacattat cgagagtatc caaaacatcg
      721 taatctgagg aaaacaattc agatgttgtt ttgataacaa agaaatacaa gtttaaatct
      781 tcatcatatt ttatattgat ttcagctttt ctttttaaaa ccgtatttat cacatcaaaa
      841 at
//
LOCUS       DACELF010000242          838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.262, whole genome shotgun sequence.
ACCESSION   DACELF010000242 DACELF010000000
VERSION     DACELF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.262"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>838
                     /locus_tag="IW687_002840"
     CDS             <1..>838
                     /locus_tag="IW687_002840"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0952037.1"
                     /translation="APQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAI
                     AFRVFKELLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVL
                     TAVGLLPIAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTEL
                     LINYEPGMQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFET
                     VVKVEKPRKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKI
                     PE"
BASE COUNT          289 a          162 c          176 g          211 t
ORIGIN      
        1 aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga
       61 tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta
      121 ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag
      181 aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag
      241 aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct
      301 ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat
      361 tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg
      421 aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt
      481 tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg
      541 gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg
      601 atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg
      661 tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg
      721 gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa
      781 cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaat
//
LOCUS       DACELF010000243          807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.263, whole genome shotgun sequence.
ACCESSION   DACELF010000243 DACELF010000000
VERSION     DACELF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.263"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            235..642
                     /locus_tag="IW687_002841"
     CDS             235..642
                     /locus_tag="IW687_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952038.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          300 a          108 c          140 g          259 t
ORIGIN      
        1 ttgttagtaa aactgtatac gaatttagat agtaaaaaaa cagtttgttc cttatataga
       61 ggcactttta ctactgatta aatgtcagat atgcttacaa atatacttat aaatatgctt
      121 acaaaattag ctaatatttc cgttgacaaa gctttgttac tattgttaaa ctaaattgac
      181 tagcaaaagt gaaacataaa tttattaaat aaatgtaaaa attaggagaa atcaatgtct
      241 tatagagtac aattaataat ttcagaagat gtagaaagtc agcaatttgg cactaatgtt
      301 attaataaag taattaatcc agctctttct attaatgcgc ctttaattcc tactgcttta
      361 tcattttctg taacagctgt ggtgtctgag atagaggata cagaaaagat aaaaatagta
      421 gaaattgaag ttttaaacaa gaatgaaaaa caaatttttt ctacgggtga agtatcggta
      481 agcttgcctc ctcaagttaa cgatataaac tttaatataa acgccagaaa tgtcttggtt
      541 gaagaagcag gagagcatta tgctgttgtt aaatttaatg gaactgagat tggaaggcaa
      601 atctttgata tcaaggtcaa caaaccagtg gaaaaaaatt aaggagacga tacggatgct
      661 tgatatagta ccatcaaata cacatcggag tggtagctta ataaattttg ctgcgatttt
      721 tgcttgcgca tcggtaattc tagcatctcc agttgttgtg gatgcaagtt ctactccgaa
      781 aactaaaaat gataatcaac ttgtaat
//
LOCUS       DACELF010000244          787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.264, whole genome shotgun sequence.
ACCESSION   DACELF010000244 DACELF010000000
VERSION     DACELF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.264"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW687_002842"
     CDS             24..716
                     /locus_tag="IW687_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0952039.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DACELF010000245          785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.265, whole genome shotgun sequence.
ACCESSION   DACELF010000245 DACELF010000000
VERSION     DACELF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.265"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            360..758
                     /gene="spxA"
                     /locus_tag="IW687_002843"
     CDS             360..758
                     /gene="spxA"
                     /locus_tag="IW687_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0952040.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          294 a          109 c          138 g          244 t
ORIGIN      
        1 aacagtatta tttaaaatga cagaaaacat tactattaaa gaaataaaaa acataaaaaa
       61 tcttaagaaa tcggtttcat ttagcttttg agataaaata atagtacagt tttatacgct
      121 gtttcatatg tttagaaaat ttgaattaga tttgaaaact ctaattttta aatgataaaa
      181 aaagcgagaa tgctggtttt actgtatatt aaccttagat aactttcatt tttttaaaaa
      241 aattatgaaa aaaaggtagc tatttaaaag ttgattatgt ataatagaaa cagatgggag
      301 attattatga tcttctgtta caaaaaagtg attcttatca cgaaaaggag tggtgcacgt
      361 tgttaacatt atatacttcc ccaagttgta cttcctgcag aaaggctcgt gcgtggttgc
      421 aagaacatca gattccattt gttgaacgta atattttttc agaaccattg aacagttctg
      481 aattaaaagc gattttacaa atgacagaag acggaacaga agaaatcatc tcgacacgtt
      541 ccaaagtatt ccaaaaactg aacatggatt tagatgatct gccgttacaa gaattattgg
      601 aacttgtgca aaacaatcct ggattgcttc gccgtccaat catgattgat gataaacgtc
      661 tgcaagttgg ttttaatgaa gatgaaattc gtcggttcct accgcgcgat gtacgacaat
      721 tagaattacg tcaagcacaa ttgatggctg gattatagaa ggataatacg aaacttggaa
      781 aagac
//
LOCUS       DACELF010000246          782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.266, whole genome shotgun sequence.
ACCESSION   DACELF010000246 DACELF010000000
VERSION     DACELF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.266"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            220..>782
                     /locus_tag="IW687_002844"
     CDS             220..>782
                     /locus_tag="IW687_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0952041.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          251 a           87 c          152 g          292 t
ORIGIN      
        1 ccgataagaa attttttcaa tgattattat tagtcttata aaaaacagaa tgtaaaattt
       61 atgtgcgcga gtatttagtt ttccattttt tgtttgatag atatatagca ttgttgaagc
      121 atgggatagt ggtgccaatt ttatcaatga ggttggtcag gcagttaaca taattggtga
      181 tgctataaat ccatttaatt ggaattggag gaattaggtg tggaaacagt gagattatgg
      241 atattcatga aaaattttcg ttcggctttg agatatgtta ttctggaatc aaaatcaggt
      301 tattatctga ttgacacaga tcactatact ttttggggag gctacctttt tccgattttt
      361 aattggataa tcccccataa ggttatgaaa attaacttgc ctgcagaaga aataaataga
      421 ttaatactca acaaagattt acaaaggaaa agagagaaag aacattctaa tgttgcttgg
      481 ataggtccag catttgcagt agtgacttct tcaattgtcg gtcctatgct agagagtatt
      541 tatagtgatt tttctattgg aatacgttat ttatttgtta ctttaagtat agctgttctt
      601 ttgtgcataa agggttatgt tagcaagaag aataaagata ttttaacact aatcggtgta
      661 aacaatttat ctagtactaa aattatactg tttcctgaat ctataaaaga aggatcaagg
      721 ttgttgataa tttttataat gcttatactg ttttctgttt tatttggggt gatgtttata
      781 ga
//
LOCUS       DACELF010000247          774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.268, whole genome shotgun sequence.
ACCESSION   DACELF010000247 DACELF010000000
VERSION     DACELF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.268"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(248..772)
                     /locus_tag="IW687_002845"
     CDS             complement(248..772)
                     /locus_tag="IW687_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952042.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DACELF010000248          759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.270, whole genome shotgun sequence.
ACCESSION   DACELF010000248 DACELF010000000
VERSION     DACELF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.270"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..714
                     /locus_tag="IW687_002846"
     CDS             <1..714
                     /locus_tag="IW687_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0952043.1"
                     /translation="QINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTP
                     LASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYL
                     KINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVN
                     GVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNI
                     EYKVLQDEEFFIFKIKIFS"
BASE COUNT          335 a           52 c          105 g          267 t
ORIGIN      
        1 caaattaatg atttgtattc tactttatta agaacaattg acgatttgga atttaaaaat
       61 aaagaaattt taaaattaga aaagttaaaa tatgactttt ttaaaggtgc atcccatgaa
      121 cttaaaaccc ctcttgctag tcttaaaata atactggaaa acatgaaata caatattgga
      181 aaatataaag aaagagatat ttatattaat gaatgtattg aaatcgttga tagtttaaca
      241 aaaaatattt ctcaaatatt atcagttcat tctatagaaa atttgaataa tgatgaagaa
      301 tatttgaaaa taaatgatac attagaagac atattaaaaa agtatgaaat attagcaaat
      361 caaaagaaaa taactgtcaa taattatata ttagacgaga atgtttatat aggaaaaaca
      421 gctttaaaga ttattctatc taatttgatt agtaatgcgg taaaatatac tgatgtaaat
      481 ggggtaatta atattgggat agttaatgat tggttatata tagagaattc atacggtaac
      541 aataaaattt cgaatatgga taaaatattt gatgttaaat ttgatttaaa taaggaaaat
      601 agtaatggat taggtttata tattgtaagt aatattctga ataattacaa tatagaatat
      661 aaagtattgc aggatgaaga attttttatc tttaaaataa aaatattttc ttaattaaga
      721 tataattttg caatagttat tgaaaaatag agacttatt
//
LOCUS       DACELF010000249          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.272, whole genome shotgun sequence.
ACCESSION   DACELF010000249 DACELF010000000
VERSION     DACELF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.272"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>741
                     /locus_tag="IW687_002847"
     rRNA            <1..>741
                     /locus_tag="IW687_002847"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          151 c          220 g          144 t
ORIGIN      
        1 caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga tcctgagtac
       61 ggcggaacac gagaaattcc gtcggaatcc gggaggacca tctcccaagg ctaaatactc
      121 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg
      181 agtgaaatag aacctgaaac cgtgtgccta caacaagtca aagcccgtta atgggtgatg
      241 gcgtgccttt tgtagaatga accggcgagt tacgattgca tgcgaggtta agttgaagag
      301 acggagccgc agcgaaagcg agtctgaata gggcgtttga gtatgtagtc gtagacccga
      361 aaccatgtga tctacccatg tccaggttga aggtgcggta aaacgcactg gaggaccgaa
      421 cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag cggagaaatt ccaaacgaac
      481 ttggagatag ctggttctct ccgaaatagc tttagggcta gcctcggaat tgagaatgat
      541 ggaggtagag cactgtttgg actaggggcc catctcgggt taccgaattc agataaactc
      601 cgaatgccat tcattcatat ccgggagtca gactgtgagt gataagatcc atagtcgaaa
      661 gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt
      721 ggggttgcac agacaactag g
//
LOCUS       DACELF010000250          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.273, whole genome shotgun sequence.
ACCESSION   DACELF010000250 DACELF010000000
VERSION     DACELF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.273"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            23..>734
                     /locus_tag="IW687_002848"
     CDS             23..>734
                     /locus_tag="IW687_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0952044.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNV"
BASE COUNT          258 a          127 c          139 g          210 t
ORIGIN      
        1 gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat
       61 aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg
      121 ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga
      181 atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc
      241 cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat
      301 tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa
      361 aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca
      421 tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta
      481 tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca
      541 taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc
      601 gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc
      661 gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca
      721 gatcagtaat gtca
//
LOCUS       DACELF010000251          701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.274, whole genome shotgun sequence.
ACCESSION   DACELF010000251 DACELF010000000
VERSION     DACELF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.274"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          271 a          100 c          103 g          227 t
ORIGIN      
        1 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
       61 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      121 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      181 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      241 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      301 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      361 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      421 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      481 aataaaaaat aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa
      541 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      601 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      661 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag t
//
LOCUS       DACELF010000252          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.276, whole genome shotgun sequence.
ACCESSION   DACELF010000252 DACELF010000000
VERSION     DACELF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.276"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            180..>635
                     /locus_tag="IW687_002849"
     CDS             180..>635
                     /locus_tag="IW687_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0952045.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT          224 a           87 c          129 g          195 t
ORIGIN      
        1 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
       61 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
      121 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
      181 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
      241 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
      301 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
      361 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
      421 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
      481 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
      541 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
      601 atggaatctg tcaggaatgc tatgaaaaag aaaaa
//
LOCUS       DACELF010000253          628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.277, whole genome shotgun sequence.
ACCESSION   DACELF010000253 DACELF010000000
VERSION     DACELF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.277"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>628
                     /locus_tag="IW687_002850"
     CDS             <1..>628
                     /locus_tag="IW687_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ0952046.1"
                     /translation="KNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFM
                     IQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFTTIPKKNSRSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGF
                     EDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRL"
BASE COUNT          234 a          123 c          109 g          162 t
ORIGIN      
        1 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
       61 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      121 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      181 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      241 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      301 tcctaagaaa tatctttacc accattccta aaaaaaattc aagatctttc agagaagctg
      361 ttaaaggaat ttttaagttc acagatatta acttagcgcg tgaggctaaa aatcgattga
      421 ttcatgatta tatcgatcaa ccaaaatatt caaaagcttg cgcatcattg gatgatggat
      481 tcgaagacgc ctttcaatat accgtacaag gaaattccca caatcgacta aagagtacca
      541 atctaattga acgactgaat caagaagtac gcagaagaga aaagattatt cgcatcttcc
      601 ccaatcaaac atcagccaat cgcttaat
//
LOCUS       DACELF010000254          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.279, whole genome shotgun sequence.
ACCESSION   DACELF010000254 DACELF010000000
VERSION     DACELF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.279"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            416..>609
                     /locus_tag="IW687_002851"
     CDS             416..>609
                     /locus_tag="IW687_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952047.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
BASE COUNT          190 a           98 c          139 g          182 t
ORIGIN      
        1 tgaaacacgt tagaggagcg aaaatggagc ctgtaaggcg actggtagcg gaacggtcgt
       61 gttagattgt gtagccgata gctgaacgaa ataaatgctg gcagcgccat tctatttcgg
      121 caggaggagg ctcaaggggt tcggggaatg aaattccctg aggtcttttt attttttcta
      181 gaggagtgct ataatcaaag aaaatgagaa aaaagagtaa aaaaatagtc gagcagtgtt
      241 gacgcactct cgacctttac ggaaactact tcacctagtt ccgtaactca agttcattat
      301 acgaatattt ttaggtttct tcaagctaga gatttttgga tgtggatttt tagagttacg
      361 tgaactctgg aaattctttc caagaatctc ttttttcgtt gaggaggagc tatttttgag
      421 taaaaaaggc ataatcgtaa atgagtggga aaaacttaac cgtatttcta acaggcggtt
      481 aaatgcacaa actatgtgca cccccttaaa aatgcaatgg tcaattgacc gaattaccat
      541 tgttggtaca ttaagggaaa atatttatta tcacactccc catgatgtgt tgattttaga
      601 ttttgaaca
//
LOCUS       DACELF010000255          594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.280, whole genome shotgun sequence.
ACCESSION   DACELF010000255 DACELF010000000
VERSION     DACELF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.280"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..>594
                     /locus_tag="IW687_002852"
     CDS             6..>594
                     /locus_tag="IW687_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0952048.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNF"
BASE COUNT          209 a           79 c           99 g          207 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat tttt
//
LOCUS       DACELF010000256          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.281, whole genome shotgun sequence.
ACCESSION   DACELF010000256 DACELF010000000
VERSION     DACELF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.281"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(195..425)
                     /locus_tag="IW687_002853"
     CDS             complement(195..425)
                     /locus_tag="IW687_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0952049.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
BASE COUNT          228 a          106 c           79 g          174 t
ORIGIN      
        1 aaactagcag ctaaaatagg caaaacagca tttttagctc cttcaattct tactgttcca
       61 tttaattgat tgccacctct tacgatgatc tcttccatgt tgttccctcc aaaaaattcc
      121 aatgagcata cacttactca aaatcttttc tattataaca tccttgcttt taaaatagta
      181 ccttttttta aaatttacag gctaacgaaa agatttcggc ataaagcgat aacttctaag
      241 aaaaaattac ttgcagtgta ccctaaaaaa attgaaaaaa gtacgattgc cattctaatt
      301 tgcgtattat gcattggtct gaaaaaagtt tcaatgcgta acgaacgtaa agaccagaag
      361 gctaagtaga taaacgccaa atgactcacg attcgaagca ccgcatcaat tccgtatact
      421 tgcataatga cgtcctttct aacgattgtt ttataataac taataaaatg ataataccat
      481 aaaatgtata aaaggaaaaa caagtatact taaaaatata ggaatcccct tacatttttt
      541 aggaaataaa tgctaaaaat atgcaaaaaa agggactgag acaaaag
//
LOCUS       DACELF010000257          584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.282, whole genome shotgun sequence.
ACCESSION   DACELF010000257 DACELF010000000
VERSION     DACELF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.282"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>584
                     /locus_tag="IW687_002854"
     CDS             <1..>584
                     /locus_tag="IW687_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0952050.1"
                     /translation="HQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER
                     PVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFN
                     SEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKN
                     FFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          201 a          108 c          108 g          167 t
ORIGIN      
        1 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
       61 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      121 cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      181 atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      241 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      301 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      361 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      421 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      481 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      541 aaccaacatg atattgatat atattttgcg gaccctggaa cacc
//
LOCUS       DACELF010000258          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.283, whole genome shotgun sequence.
ACCESSION   DACELF010000258 DACELF010000000
VERSION     DACELF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.283"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..476
                     /locus_tag="IW687_002855"
     CDS             <1..476
                     /locus_tag="IW687_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0952051.1"
                     /translation="RGYNSTKNAYFYGLKIHIIVTKTGFPVTYSITDPSVHDVKVLET
                     LSEEANLPNILGDKGYVSHKIHEKLALKGITVLVPSRKNMDKLGKIDHRLLTRQRKAV
                     ETVFSSLERLGFQNFKSRSIQGLESKFESILLSYSILLSRAQQHFEGTLRYSLGY"
BASE COUNT          203 a           94 c          105 g          176 t
ORIGIN      
        1 gtaggggcta taactcaacg aaaaacgctt atttttatgg tcttaaaatt catattatcg
       61 tcactaaaac aggctttcca gttacttatt caatcacgga tcctagtgtt catgatgtga
      121 aagttttaga gactttatcg gaagaagcaa accttccaaa tatccttgga gataaaggct
      181 atgtcagcca taaaatccat gaaaagcttg cccttaaggg cattacagtc ttagttccgt
      241 cacgaaaaaa catggataaa ctagggaaga tagaccatcg attacttact agacaaagaa
      301 aagcagtaga aactgttttt tcttctctcg aaagactagg ttttcaaaat ttcaaatcac
      361 gttctattca agggctagaa agtaagtttg aaagcatatt attatcgtac agtattcttt
      421 taagccgagc acagcaacat tttgaaggga ctttgagata ttctttaggg tattaaaatt
      481 aactagcacc acgggtatat ttttattata cacagcttgt cgcttggata gaactttagt
      541 ataattaaac gaaaaagagg taaatgaaat ggaaatgt
//
LOCUS       DACELF010000259          545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.285, whole genome shotgun sequence.
ACCESSION   DACELF010000259 DACELF010000000
VERSION     DACELF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.285"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..210
                     /locus_tag="IW687_002856"
     CDS             <1..210
                     /locus_tag="IW687_002856"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="catalyzes the addition and repair of the
                     3'-terminal CCA sequence in tRNA; these proteins belong to
                     the CCA-adding enzyme subfamily 2 which does not have
                     phosphohydrolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0952052.1"
                     /translation="AVGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAE
                     KAVVEQRIKNDKKQVLDFLSKEKLI"
     gene            364..>545
                     /locus_tag="IW687_002857"
     CDS             364..>545
                     /locus_tag="IW687_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952053.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFH"
BASE COUNT          206 a           80 c          110 g          149 t
ORIGIN      
        1 gccgtaggag aatcctatga tcgtttacct atcaagtcat taagcgaatt agcagtagat
       61 ggtaaaatct tgctagaaga aactggaaaa aaacctggta agtgggttag tgaagctttg
      121 caattagctg aaaaagccgt cgtagaacaa agaataaaaa atgataaaaa acaggttttg
      181 gattttttat cgaaagaaaa attgatttaa aaaacactag actgtatctg ctcagctttg
      241 ttggctactt aaagtgggca gatacgtcat tagaagaaaa aacataagat gtgaccactt
      301 acataaattt gtgctaaact gaggtagtac aagaataaat tacatcataa aggagtcttt
      361 ttaatgagta aagaaatgac agcgctaaaa ttttatttta gaaatggaga aacctggacg
      421 atcaaccgcc gtcatatcgg cgatctttgg atcaaacaaa tcacaacaag ttttggacga
      481 atcaatggca gtgaatttgt tgaaatccat ccgtgtgctg gatttaaaat cgaaatcttc
      541 catga
//
LOCUS       DACELF010000260          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.286, whole genome shotgun sequence.
ACCESSION   DACELF010000260 DACELF010000000
VERSION     DACELF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.286"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..136)
                     /locus_tag="IW687_002858"
     CDS             complement(<1..136)
                     /locus_tag="IW687_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0952054.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     D"
     gene            complement(123..452)
                     /locus_tag="IW687_002859"
     CDS             complement(123..452)
                     /locus_tag="IW687_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952055.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          165 a          115 c           55 g          208 t
ORIGIN      
        1 cgtctaatgc taaaacatct aatcctttac aatttccgcc tcttctaaac gtcacaaatc
       61 ctttgtaatc ccaacgttgg aaagaaacct taatcgtatg tgtgcccaat cgtatattcg
      121 gatcataatt tttcatttgt tgtaacctcc tcataatcag ttagtactat aaaggtatct
      181 ttgtttttat ccaaaaaatc taataaatct tttgtagaag atatctcttc atcaacttca
      241 ccataataaa taccttcgat aattgttgct atatctgaaa aactaatttt tactggacat
      301 ttatcgtcta ccgcattcaa taccgacaaa attgcttctt tttctgcaat ctctcgtttg
      361 aatttctttg gagctaatcg catgacactc aagtcatttt caagcttttc aatccatgca
      421 gttttactca ttttcgcaaa tttttgaatc attcctctac cacctttgct atctactaaa
      481 ttagttttac ttcataacga atatctttat cgtttttcgt tcactcaaaa atatataaaa
      541 taa
//
LOCUS       DACELF010000261          537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.287, whole genome shotgun sequence.
ACCESSION   DACELF010000261 DACELF010000000
VERSION     DACELF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.287"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..>537
                     /locus_tag="IW687_002860"
     CDS             10..>537
                     /locus_tag="IW687_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ0952056.1"
                     /translation="MTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDN
                     PLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMS
                     IRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYT
                     RRNEIELVVKEMAEQV"
BASE COUNT          194 a           89 c          127 g          127 t
ORIGIN      
        1 atcttaaaag tgacccgatc actgaatctt tttacgactg aaggaacacg aagtcaacgg
       61 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgattgct
      121 acagtaggtg tcggagataa tccccttttg gctaaactag ccttagacaa cgaagcaaag
      181 cataatgaag gatttgttgc ggaatggacg tacgaaaacg ttcctgaaaa agtatggaat
      241 attcctgaaa tgacggactt ttggggaatt ggctctcgaa tgaaaaaaag actcaatcag
      301 atggggatta tgagcattag agatcttgct aactggaatc cttatacgat taagaataga
      361 ctaggtgtga ttggcttaca gctgtacttc catgctaacg gaatcgatcg aacagatata
      421 gcaatcccgc cagaaccgac aaaggaaaaa tcttatggta attcacaagt gttaccacga
      481 gattatacta gaagaaatga aatcgagctg gtggtaaaag aaatggcgga acaagta
//
LOCUS       DACELF010000262          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.288, whole genome shotgun sequence.
ACCESSION   DACELF010000262 DACELF010000000
VERSION     DACELF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.288"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          208 a           61 c           75 g          192 t
ORIGIN      
        1 atgttaattt atactaaatt aaataaagta ctacctttaa taggtagtac tttattatca
       61 agccattatt ccttcttcta ctagttcttt ttgtcacata attttgcaat caataaatgt
      121 tgtgcaataa gtataagaat tccattttct aaatttgaaa ggtgatccat aatttcatgt
      181 tcaagtagtt tattaattgt ccacttgtcg cttcatctaa aaattttata ggttttagga
      241 atgctattcg cttattttag gaagatttta actgtttgct atagatatat atcatactag
      301 tatcgtaata aaagtactct aaaagaagaa ttttcaaata cgcaaaatta agtacgaaag
      361 agttgaaaca aatttggaga aaatgagcat agaatgtctg atgagttaaa tagagattac
      421 gtccatataa ttgtgtaaaa gtggagcaaa attaggaatt acaaacaaaa cactaaatta
      481 gtttatataa ttttccgtaa tatttaatat atatgaaaag gaaaacgtag aaaaaa
//
LOCUS       DACELF010000263          520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.290, whole genome shotgun sequence.
ACCESSION   DACELF010000263 DACELF010000000
VERSION     DACELF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.290"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..>520
                     /locus_tag="IW687_002861"
     CDS             81..>520
                     /locus_tag="IW687_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0952057.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEP"
BASE COUNT          197 a           96 c           92 g          135 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat
//
LOCUS       DACELF010000264          519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.291, whole genome shotgun sequence.
ACCESSION   DACELF010000264 DACELF010000000
VERSION     DACELF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.291"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..425
                     /locus_tag="IW687_002862"
     CDS             135..425
                     /locus_tag="IW687_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952058.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          184 a           76 c          120 g          139 t
ORIGIN      
        1 catgtccttc atcttcgcag gaagtgccaa tatgatttta agaaaggtaa tagacaaaaa
       61 gtcaaaaaga taaaacagag tttcttcttt acaaagggaa tgtatgttcg tataatggta
      121 ttgaggtgaa ggttatggag aaaaaatatg gaacagtaga taagatccgt actttgcaag
      181 ttagtgccag accgttgatc cgtttcagtt taaatggggt caattgtttg attgcttccc
      241 atagtttgaa cttcctagct gaagtagacg aagggatgaa gttagtcgtt acaggttatt
      301 ataataatag aaagcaattt gttgtgagat cctatcatgt gttagggaaa cccaaaattg
      361 tggtagaata tgagaaaagt ctatacccaa gaaaaaaggt ccgacaagat gaaagaataa
      421 actagaaaac aagcggctga gacaaaaatg tcctagccgc ttgttttgga aagatggatg
      481 cagacgatgg gatttgaacc cacacgagca tacgctcac
//
LOCUS       DACELF010000265          519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.292, whole genome shotgun sequence.
ACCESSION   DACELF010000265 DACELF010000000
VERSION     DACELF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.292"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW687_002863"
     CDS             168..362
                     /locus_tag="IW687_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952059.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          221 a           64 c           83 g          151 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatc
//
LOCUS       DACELF010000266          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.293, whole genome shotgun sequence.
ACCESSION   DACELF010000266 DACELF010000000
VERSION     DACELF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.293"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            87..305
                     /locus_tag="IW687_002864"
     CDS             87..305
                     /locus_tag="IW687_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952060.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          160 a           86 c           94 g          175 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcag
//
LOCUS       DACELF010000267          492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.294, whole genome shotgun sequence.
ACCESSION   DACELF010000267 DACELF010000000
VERSION     DACELF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.294"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            229..>492
                     /locus_tag="IW687_002865"
     CDS             229..>492
                     /locus_tag="IW687_002865"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0952061.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          160 a           97 c           75 g          160 t
ORIGIN      
        1 ataaatattt tcaaaaacct tttcataatc ctcacccttt cttacactga taaatcatgc
       61 gtctaatatt caaattatac tcgatatgct ttagctaatc agccaactct gtttgtctac
      121 tatcattata tcaatagaca atatctgctc ccgtctttat gatcgtgcca aagcaatcaa
      181 aagaaaattg cgaatcactt agatcgaaag tttactcaaa caaaagcatt gtcagcggtt
      241 gtcacggatt taacttatgt tcgggtcggg acaaattggg cttatatttg tttgatgatt
      301 gatttattca atcgtgaaat tattggccat tcttgcggaa ataaaaaaga tgcgcagctt
      361 gtaaaaaggg ccattcaaag cattccttac tcacttcaag aaatcgaact cttccatacg
      421 gatcgtggaa aagaatttga taaccaaacc attcagaatc tcatgaatgg tttggttatc
      481 aaattccttt cc
//
LOCUS       DACELF010000268          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.295, whole genome shotgun sequence.
ACCESSION   DACELF010000268 DACELF010000000
VERSION     DACELF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.295"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>482
                     /locus_tag="IW687_002866"
     CDS             <1..>482
                     /locus_tag="IW687_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ0952062.1"
                     /translation="FRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGSYDRT
                     VKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIADLIEK
                     MYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMDATYIPLKRQTVSKEAIYI
                     A"
BASE COUNT          147 a          112 c           99 g          124 t
ORIGIN      
        1 ttccgtgtcc atttggaaaa agctgtcaat acgcttctca aaacggagtt aacggctttc
       61 ctcgattacg aaaagtacga tcgcattggt tttaacacgg gtaattctcg taacggctcc
      121 tatgaccgta cggtcaagac cgagtacggg gaacttcatc tccagattcc gcgcgaccgc
      181 aacggcgagt tcaagcaaca gactgttcct gcttatagac ggacgaatga taccttagag
      241 gagaccgtca ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt
      301 gagaaaatgt atgggcatca ctacacgccc caaaccatgt ccaacatgac taaagtgctg
      361 actgaagaag taaatgcatt taaagttaga actctaaatg ataagtatgt cgctattttc
      421 atggatgcta cttatattcc tttaaaacgg caaaccgtct ccaaagaagc aatctatatt
      481 gc
//
LOCUS       DACELF010000269          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.298, whole genome shotgun sequence.
ACCESSION   DACELF010000269 DACELF010000000
VERSION     DACELF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.298"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..430)
                     /locus_tag="IW687_002867"
     CDS             complement(<1..430)
                     /locus_tag="IW687_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0952063.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACELF010000270          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.299, whole genome shotgun sequence.
ACCESSION   DACELF010000270 DACELF010000000
VERSION     DACELF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.299"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          144 a           91 c           67 g          151 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaacact
       61 ttatacataa aaaaagtcaa gtattagaaa aaagttcaaa agatcaagtg tgcgcttcgc
      121 tcccactccc ctcgacgttt tagtagcctt tccctcactt cgttcagtcc aagccaacta
      181 aaaagttttc gggatacatt ctccttctct tcctaataat caattaaaat tttaatcttt
      241 atatttctgc taatcactca ggaaacagta cagaaaaaca aacacgtatc atagatataa
      301 atgtaatggc atagtgcggg ttttattttc agcctgtatc atagctaaac aaatcgagtt
      361 gtgtgtccgt ttttgggcgt tctgctagct tgtttaaagt ctcttgaatg aatgtatgtt
      421 ctaagtcaaa aaactctgac agcgccttta tat
//
LOCUS       DACELF010000271          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.denovo.302, whole genome shotgun sequence.
ACCESSION   DACELF010000271 DACELF010000000
VERSION     DACELF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.denovo.302"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..417
                     /locus_tag="IW687_002868"
     CDS             <1..417
                     /locus_tag="IW687_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0952064.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVDG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          156 a           75 c           86 g          115 t
ORIGIN      
        1 acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttggag aaatcgaaaa cgcaaacttg
      121 catgtggatg gatggcacat tgctaattat aaatacgagt atattttcat tatggattac
      181 aatactggaa aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
      241 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctaataaga aagtctatgt aatgatgcgt gcaacgaatg atccagaagg gaacactaaa
      361 ggcggagcac aagattttca tgataaacgc tggtatttaa atattccgca acgataaaaa
      421 aagcccctcg at
//
LOCUS       DACELF010000272        23728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.041, whole genome shotgun sequence.
ACCESSION   DACELF010000272 DACELF010000000
VERSION     DACELF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.041"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW687_002869"
     CDS             289..564
                     /locus_tag="IW687_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952065.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW687_002870"
     CDS             577..2655
                     /locus_tag="IW687_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0952066.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW687_002871"
     CDS             2815..4701
                     /locus_tag="IW687_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0952067.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW687_002872"
     CDS             4713..5660
                     /locus_tag="IW687_002872"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0952068.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IW687_002873"
     CDS             5680..6207
                     /locus_tag="IW687_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0952069.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IW687_002874"
     CDS             complement(6270..6923)
                     /locus_tag="IW687_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0952070.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IW687_002875"
     CDS             7055..7897
                     /locus_tag="IW687_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0952071.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8055..8951
                     /locus_tag="IW687_002876"
     CDS             8055..8951
                     /locus_tag="IW687_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0952072.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8954..9667
                     /locus_tag="IW687_002877"
     CDS             8954..9667
                     /locus_tag="IW687_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0952073.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9687..10205
                     /gene="msrA"
                     /locus_tag="IW687_002878"
     CDS             9687..10205
                     /gene="msrA"
                     /locus_tag="IW687_002878"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0952074.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10202..10429
                     /locus_tag="IW687_002879"
     CDS             10202..10429
                     /locus_tag="IW687_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0952075.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10581..11132
                     /gene="lepB"
                     /locus_tag="IW687_002880"
     CDS             10581..11132
                     /gene="lepB"
                     /locus_tag="IW687_002880"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0952076.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11215..11757)
                     /locus_tag="IW687_002881"
     CDS             complement(11215..11757)
                     /locus_tag="IW687_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0952077.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11994..12392)
                     /locus_tag="IW687_002882"
     CDS             complement(11994..12392)
                     /locus_tag="IW687_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0952078.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12652..13512
                     /gene="ylqF"
                     /locus_tag="IW687_002883"
     CDS             12652..13512
                     /gene="ylqF"
                     /locus_tag="IW687_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0952079.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13505..14272
                     /locus_tag="IW687_002884"
     CDS             13505..14272
                     /locus_tag="IW687_002884"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0952080.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14328..15185
                     /gene="dprA"
                     /locus_tag="IW687_002885"
     CDS             14328..15185
                     /gene="dprA"
                     /locus_tag="IW687_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0952081.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15307..17385
                     /gene="topA"
                     /locus_tag="IW687_002886"
     CDS             15307..17385
                     /gene="topA"
                     /locus_tag="IW687_002886"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0952082.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17354..18730
                     /gene="trmFO"
                     /locus_tag="IW687_002887"
     CDS             17354..18730
                     /gene="trmFO"
                     /locus_tag="IW687_002887"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0952083.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18928..19833
                     /gene="xerC"
                     /locus_tag="IW687_002888"
     CDS             18928..19833
                     /gene="xerC"
                     /locus_tag="IW687_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0952084.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19870..20418
                     /gene="hslV"
                     /locus_tag="IW687_002889"
     CDS             19870..20418
                     /gene="hslV"
                     /locus_tag="IW687_002889"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0952085.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20432..21832
                     /gene="hslU"
                     /locus_tag="IW687_002890"
     CDS             20432..21832
                     /gene="hslU"
                     /locus_tag="IW687_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0952086.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21850..22641
                     /gene="codY"
                     /locus_tag="IW687_002891"
     CDS             21850..22641
                     /gene="codY"
                     /locus_tag="IW687_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0952087.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22723..23598
                     /locus_tag="IW687_002892"
     CDS             22723..23598
                     /locus_tag="IW687_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0952088.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8212 a         4091 c         4999 g         6426 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
     8041 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
     8101 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
     8161 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
     8221 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
     8281 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
     8341 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
     8401 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
     8461 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
     8521 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
     8581 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
     8641 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
     8701 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
     8761 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
     8821 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
     8881 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
     8941 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
     9001 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
     9061 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
     9121 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
     9181 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
     9241 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
     9301 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
     9361 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
     9421 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
     9481 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
     9541 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
     9601 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
     9661 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
     9721 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
     9781 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
     9841 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
     9901 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
     9961 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    10021 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    10081 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    10141 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    10201 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    10261 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    10321 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    10381 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    10441 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    10501 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    10561 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    10621 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    10681 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    10741 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    10801 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    10861 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    10921 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    10981 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    11041 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    11101 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    11161 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    11221 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    11281 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    11341 tgcaccaaac aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat
    11401 caataattgg gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc
    11461 attaataaat ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc
    11521 aaatatcgtg aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc
    11581 atatagataa cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg
    11641 accaatcgct atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa
    11701 caatcttgca ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat
    11761 attctctcct ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac
    11821 acattggtga tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt
    11881 ttataaactg taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc
    11941 gtatcatccg cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc
    12001 ttcatccata cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt
    12061 tcttcttcag gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt
    12121 tgagcaaatt tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt
    12181 gtttttttat tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag
    12241 agaagcatta tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat
    12301 tcttcatttt ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca
    12361 acaggaaaat taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg
    12421 tctatcatct cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa
    12481 ataaaaattt gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc
    12541 acttaactaa tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa
    12601 aaaacaagta aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc
    12661 caatggtttc cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat
    12721 gtggacatcg tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg
    12781 ctagatcaga tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca
    12841 gatccacaac agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta
    12901 gctatcacgg cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca
    12961 ttgaaagaaa aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca
    13021 atggtcttag gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag
    13081 aaaattgcac agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta
    13141 ggtaatgagt tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa
    13201 gaaatcggca aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat
    13261 gatttagcga tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat
    13321 cgttatggtt tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt
    13381 acagcaaaac gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat
    13441 attcgtcaag gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa
    13501 agtgatgact gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga
    13561 tgatcctaca ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc
    13621 ttcttttgaa cgacgattga aaaaggaagc attaattaag gaaaaatatg aagaaatgtt
    13681 ttgctttgaa aagcaagggt acgcacaagg tcaccaatgg attgccggca ttgatgaagt
    13741 aggaagagga ccattagctg gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga
    13801 gatcatatta gggctgaatg actccaaaca attatcagag aaaaagcgag aacttcttta
    13861 tcaacaagtt caagaaaaag ccctggcaat cgggatcggg atcgttgatc agcagacaat
    13921 cgatgatgtg aatatttatg aagcaagtaa attagcgatg atccaagcag tagaaaaatt
    13981 gtccattgta ccaagctatt tgttgatcga tgcgatgacc ttggatttgc ctattcagca
    14041 ggaaaaaata atcaaagggg atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc
    14101 taaagtatat cgagaccaat tgatgaaaga atatgacgtg ctttatcctg gatatggttt
    14161 ctcgcataat gcaggttatg gaacaaagga acacttagaa ggacttgaac gacaaggaat
    14221 tacgccaatc catcgtaaaa gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc
    14281 cgcttttacg tatcttatta gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag
    14341 aagaaaattt attgaaactt gcttatacaa aaggaatcag tagtcaagga aaatgggcac
    14401 ttgctaactg gatgatgcag tatccttata aagaaatcgg atttgaagac atcattaaga
    14461 taggtaaaat aaccaaacac cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta
    14521 actggggcaa aatccaatcg aaacagtcgt ttatcacttg ctttgatccc aaatatcccc
    14581 ctcagctgct tcacttgacc tatccgccaa ttgtactgtt ttataatggt gatctatctt
    14641 tactttcttg taacatgtta agcgttgttg gtggaagaca cacttctgct ttagccaaaa
    14701 aaacagttta ttatttacta aaaccagtga tagaagctgg atatgtgatc gtcagtggct
    14761 gcgcgaaagg gatagataca tacggtcacc aagcagcaat ctctcatggt ggacggacaa
    14821 tcgcagtcat tggaacaggg atagagcaag tctatcctgc tgaaaatcgt gacttgcaag
    14881 aggtgattgc aaatgatcat cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa
    14941 agcattgttt tccaatgaga aatcggatta tcgcagcttt gtctaaagga acatgtgtct
    15001 ttgaagccaa gaaaaaaagc ggctctctga taacagcaca gcaagcattg gatcttggaa
    15061 gaacagtttt ttctgttcca ggaaatattt taacagaaca ttctaccgga tgtcatcatt
    15121 tgatccaaga cggtgcagtt tgcacggttt cgggtgaaga tatcttggca gaatttaaag
    15181 aataacacca tacttttcat ttgcttttct tgacaaacgt tttctgaatg gatatgatta
    15241 gctacgattt gtatgaatcc tcttttaata gtaggaacac gacaatggaa aggagtccat
    15301 tcagtaatgg cgtataaata tttagtgatt gtagaatcac ctgcaaaagc caagacaatt
    15361 gagaaatatc ttggacgaaa ctataaagta atggccagtg ttggccatat aagagattta
    15421 ccgaaaagta aaatgggtat cgattttgaa aacaattatg aaccccatta tatttctata
    15481 cgcggaaaag gcgatgtcat caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt
    15541 tacttggcaa gtgacccgga tagagaagga gaagcgattg cttggcattt agcgtaccta
    15601 cttgggttgg atctgaaaga aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca
    15661 gtcaaagcag cttttaagga accaagaacg atcgatgtag atttagtaga tgcacagcaa
    15721 gctcgtcgta ccttagacag aatcgttggt tattcgatca gtcctattct ctggcgtaag
    15781 gtcaagaaag ggttaagtgc aggacgtgtc caatctgtcg ctttaaaaat tattattgac
    15841 cgtgaaaaag agatccgaga atttgttcca gaagaatatt ggagcatcga cggtaatttt
    15901 aaaaaagctc gcaagaaatt caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa
    15961 ttaccagatg cacaaagtgt aaaagaagtc actgctagaa ttgatggaac agacttcgaa
    16021 gttaaaaaag tagaaaagaa agagcgcaaa agaaaccctg cgaatccttt cactacaagt
    16081 agcctgcaac aagaagctgc ccgtaagtta aactttcgaa caagaaaaac catgatggta
    16141 gcacagcaat tatatgaagg aatcactttg ggtaaacaag gaacagtcgg cttgattaca
    16201 tacatgcgta ctgattctac ccggattgcc gatacagcaa aagcagaggc cgcagcattt
    16261 attgaagata cgtatggaaa agaattttct agacatgatc accgaaagat caaaaatgct
    16321 caaggcgcac aagatgccca tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat
    16381 gaactcaaac aatatttaga taaagatcaa ttgaaacttt atacattgat ctggtcacgt
    16441 tttgtggcta gtcagatgac accagctatt ttagatacaa tgaaagtgac attagaacaa
    16501 aatggtgtca tcttcattgc gaacggttca aaaatcaaat tcaaaggatt catgcaagtg
    16561 tatgtggaag gtagagatga cggcaaagaa gagaaagaaa atattcttcc agaacttgaa
    16621 gaaggcgatg tagtccaatc tgttgatatt gaaccaaaac aacattttac tcaaccacca
    16681 gcacgtttta gtgaggctac gttgattcgt gcactagaag aaaacggggt agggcgacct
    16741 tccacttatg caccaacact tgatacgatc caaagacgct actatgtaaa attgacacaa
    16801 aaaagatttg agccgactga actaggagaa attgtcaatt cattaatctg tgaattcttc
    16861 cctcaaatcg tcgatatcca tttcacagca gaaatggaag gagatttgga taaaattgaa
    16921 gaaggcacgg aagcgtgggt caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa
    16981 ctgacgaatg cggaagaaaa aatcgaaaaa attcaaatca aagatgaacc agcaggattc
    17041 gattgtgatg tctgtggaca tccgatggtg ataaaattag ggaaatatgg gaagttttat
    17101 gcttgcagta attttcctga ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg
    17161 acttgcccag tatgtcatga aggacaagtc attgaacgaa aatctaagaa aaacagacta
    17221 ttctatggtt gttcccgttt cccagaatgt gaattcactt cttgggataa accagtgggc
    17281 agaaattgtc caaaatgtga cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa
    17341 gtagtctgtg taaatggaga ttacgaagaa gacatccaaa aataatagaa aagaggttct
    17401 ttcattgaaa atggtaaatg ttgttggagc agggcttgcc ggaagtgaag ctgcttggca
    17461 gatcgcacaa gcaggagtcc ctgtccgttt atatgaaatg cgtccagtca aatcgactga
    17521 ggcgcatcac acaagtaatt ttgctgaact agtatgttcc aattcattaa gaggaaacag
    17581 tttggctaat gcagtagggt tattgaaaga agaaatgcgc cgattgaatt cggtagtaat
    17641 cacttcggca gatgaaacag cagtgccagc tggtggtgcc ttagctgttg accgcgattc
    17701 tttttctgaa acaattaccc gaaaagtcaa agagcatcca ttagtaacag tgataaacga
    17761 agaaattact gaaataccgg aaggaatcac cgtagtagca acaggacctt tgacttctca
    17821 tccattagca gaaagcatca aagcattcaa tggttcagat ggtttttatt tctatgatgc
    17881 agctgcacca atcgtagaca aagctacgat cgatatggat aaagtatatt tgaagtcccg
    17941 ctatgacaaa ggagaagcag cttacttgaa ctgtccgatg acagaagagg aattcaaagc
    18001 tttccatgag gcactaatca atgccgaagt ggcagaactt aaatcttttg agaaagaaaa
    18061 atattttgaa ggatgtatgc caattgaagt aatggcacag cgaggattca aaacgatgct
    18121 tttcggacct atgaaaccag ttggattgga agatcctaaa actggcaaac gtccttatgc
    18181 tgttatccag ctgcgtcaag acaatgctgc agcttcttta tacaacatcg tgggctttca
    18241 gactcacttg aaatggggcg agcaaaaacg tgttttccgg atgattcctg ggttagaaaa
    18301 tgctgaattc gttcgctatg gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt
    18361 gctgaaaccg acttaccaat caacgaaacg cgaagatctg ttctttgcag gtcagatgac
    18421 tggtgtagaa ggctatgttg aaagtgcagc gagcggacta cttgctggaa tcaacgccgc
    18481 acgcttagca aaagaaatgg aaccagtcga gtttccacaa gaaacagcta ttggaagtat
    18541 ggcttactac attactcatg cggaaggaaa acatttccaa ccgatgaatg ccaactatgg
    18601 tctgtttcct gaactgccag agcgcattcg agacaaaaaa gcaagatatg aagcaattgc
    18661 acaacgagct ttaaaagcta atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc
    18721 aaatgagtaa ggaaactcca tgaagagggt gagacggata gtctcgctct ttttcttgcg
    18781 tttttcttaa gaatttctca aacaaaatgt gaggaattgc gccaattgtg aacaaactta
    18841 gaaatattgg ttaaattctg acaatttatt tgtcttttta taaacgctgt gctaaattat
    18901 tctctgggac aaaaggaagg tgagaaaatg acagaagcca cgtgggaaca aaaatttttg
    18961 cgttatttga ttgtcgaaag aggatattca gaaaaaacga aagaagcata tgaagaagac
    19021 attctccatt tcaaacattt tttggaggaa tctggagacg ctgatctgct gaaggcggaa
    19081 tattttacgg ttcgtgtcta tttgagtgca ttagctgatc atcgtcctag ctatagcaga
    19141 aattctatca gtcgcaagat agccagccta cgttcgtttt atcagtatct tctcaaagaa
    19201 gaagtgataa aagaaaatcc attttcttac gttcatctca aaaagaaaaa tttgagactg
    19261 ccacgttttt tttatgaaaa tgaaatgcaa gcactttttg acagcgtagc aggagatagt
    19321 cctttggatt tgaggaaccg cgctttactg gaagtccttt acgggtcagg catccgttta
    19381 agtgagtgta gcagtttgtc ggtatcagac attgattttg attcagaggt catgttgatc
    19441 catggtaaag ggaacaaaga gagatatgct cctctaggtt cttttgctca agatgctctg
    19501 caagaatatt tcagggaagg ccgccaggta ttgatgacaa aatatcacga agagcatgat
    19561 atcgtttttg tgaatcatca tggaaaacca atcacgccta ctggaattga atatgtcttg
    19621 aatcaaatga tcaaaaaaag cagtttggat agtgatatcc atcctcacat gctgcgccat
    19681 acatttgcta ctcatttatt aaataatggg gcagatttgc ggacagtcca agaattgcta
    19741 ggacatgcca atttatcgac tacccagatc tatgcccatg taacaaaaga aagtttacaa
    19801 aaaaactatc gttctttcca tccaagagcg taaaaataag gaaaagaaca aatttaggag
    19861 gaaacaaaca tggtcgaatc accatttcat tcaaccacta tctgtgccgt cgaaaaagac
    19921 gggaaatttg ctatggctgg agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa
    19981 ggcactgcaa agaaagtacg acgtatctac aatggagaag ttgttgtagg tttcgccgga
    20041 agtgttgcag atgcgtttac tttagaagaa aaatttgaag gcaaactaaa cgaatataac
    20101 gggaatctac aacgcgcagc tgtcgaatta gctcaagaat ggcggacaca gcaatctatg
    20161 caaaaacttg aagcgatgct gatcgtaatg aatgataaag aaatgcttct tgtttcaggt
    20221 acaggagaag ttatcacacc agatgatggt atcttagcta tcggatcagg agggaatttt
    20281 gcgttggctg cagcccgagc aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa
    20341 attgcagaaa atgcgttaaa tattgccgca gatatctgta tctttactaa tcataatatt
    20401 atcgtagaag aactgtaaga ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca
    20461 aatcgtaagt gaattagatg aatatatcgt aggacagcac gcagcaaaaa aatctgttgc
    20521 cgttgctttg cgtaaccgct atcgccgttt gcagttagat gaaaaaatgc aacaagatat
    20581 tacaccaaaa aatatgctga tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg
    20641 acgtttggca aaaatcgtca atgcaccatt tgtcaaagtc gaagcaacaa aatttactga
    20701 agtcggttat gtcggaagag atgtagaatc aatggttcgg gatttagtag aaaatgcgat
    20761 ccaaatcgta gaaaaagagc agtacagccg tgtatacaca agtgctaaaa aaaatgcaga
    20821 aaaacgtttg gtaaaaatct tggtacctgg tatcaaaaaa gaacagaaac aatccactaa
    20881 tcaatttgaa caaatgatga atctttttaa tgctagtcag caaaataccg ctgaaccgca
    20941 agaagaagtg acagaagata ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa
    21001 gggtttactg gatagtcgtg aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc
    21061 aatgatgaac aatggattgg agcaaatggg gatcgaccta aatgaaactc taggtgcttt
    21121 gacaccgaag aaaaaagtgg aacggacggt aacagtcaaa gaagctttag agttattgat
    21181 caaggaagaa tcagcaaaat tagtcaagga tgctgatatc catagtgagg caatcaaatt
    21241 agcggaagaa agtggaatta tttttatcga tgaaatcgat aagatcactt ctaaatcaca
    21301 gcaatctgga gaagtatcca gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg
    21361 ttcacaagta aacacaaaat acgggatcat ccaaacagat cacgtcctat tcattgcttc
    21421 aggagctttc catttgtcta aaccaagtga tctaattcca gaacttcaag gacgcttccc
    21481 gatacgggta gaattagatg atttgacagc tgaagatttt gtgaagattc tgaccgaacc
    21541 aaataatgct ttagtaaaac aatacgtcgc gttgatcggt acagaaaatg tgtcagttac
    21601 gtttacaaaa gaagcaattg aacggatcgc tcaaatcgct ttcgatgtca accgtgacac
    21661 agataatatc ggcgcacgca gactgcatac cattttggaa aaactactag aagatctatt
    21721 atttgaagca ccagatatgc aaatgggaga aataacgatc acagaagcat atgtggatga
    21781 caaacttggc tcaattgcgg caaatgaaga tttaagtcgc tatattttat aaaaaatcgg
    21841 aggacgacaa tgacagactt gctaacgaaa acccgcaaga taaacacact tttgcagcaa
    21901 aagaatactt ttgacatgca agcagatttg ccttacgata aaatggctgt cacactaggt
    21961 ggtattcttg acagcaacac ctatatcata ggaagtgacg gagtattact aggttataat
    22021 gaacgtcacg atgtgaacaa tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct
    22081 gaatcttata ctgatacagt ggcacagttg atgaaaactg aagctaatat cccaattact
    22141 agtgatatta ctgcatttcc ggtagagtca agagagaaat atccatttgg tctgacgact
    22201 gtcgttccga tctttggagc tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa
    22261 caatcgtttg cagatgacga tctagtctta gcagaatatg gagctacggt tgtcggtatg
    22321 cagatccttt atcagaagtc tcgaagcatc gaagcagatg tacggagtgc aacggctgtc
    22381 caaatggcaa tcaacacatt atcttatagc gaattgaagg ctgtacaagc tatctttgaa
    22441 gcattggatg gagatgaagg tcgtctgact gcttctaata ttgccgatag tatcggcatc
    22501 actcgttctg tgatcgtcaa tgctttacga aaattggaat cagctggaat cattgaatct
    22561 cgttcgttag gcatgaaggg aacttattta aaagtattga attcccgttt taaagacgag
    22621 ttagcaaaac acagttatta gaagtaaggg gactggggca gatagaaatg tttgttccag
    22681 ccttcccatt ttaaaaagta agtcgaagtt ggaggaatgg caatgactgt tattattcaa
    22741 aatgaccagc tgatcgccga aatcgctgaa cacggtgcgg aattgatcag tctgaaatca
    22801 aaagaacatg gtttagagta tatctggcaa ggtgatccag cgtattgggg cagacatgca
    22861 ccggttttat tcccttttgt tggacgttta aaagatgatc agtatgttta tcaagggaaa
    22921 acatatcaaa tggggcaaca cggatttgct cgggatatgg attttgaagt cattgaacat
    22981 ggacaagaaa tggctagttt catcttaagg agtacgccgg agacattaga aaagtatcca
    23041 tttgattttg aattagtgat cagctatgaa ttaggaggag acggcatcac ggttcattat
    23101 caagtggcaa ataccggtga aaaagaaatg ttcttttcta tcggtggaca tcctgctttc
    23161 aatgtacctc ttgaagaagg attgtcattt gaagatttct atctggcatt ttctccattg
    23221 aaatcaagat tacgcctacc attgaaaggc ccattcatcg atatggatca aaagacgtta
    23281 ggtcagacaa atacgaatct ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt
    23341 tttgaaacaa ggggtctaaa tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta
    23401 actttaagct ataaagatat gccttatgta gggatctggt ctccttatcc aaaagaagcg
    23461 cctttcgttt gtatcgaacc gtggtgcgga atcgctgaca cggtgaacag tagtggaaac
    23521 ttgatggaaa aagtagggat caatcaacta ggtgcccatg aaatcttcaa gacaaaatat
    23581 tccatcacag tgaagtaaga ggttgtgacc gagaagttag cagggaacac cgtttaccaa
    23641 ggtgtgtaaa aaagctatcc tgtaagtaat aagggagtgt gacatcgatt tgtggctctt
    23701 tgtcaataag gactgatgag ttgtgcaa
//
LOCUS       DACELF010000273        23727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.042, whole genome shotgun sequence.
ACCESSION   DACELF010000273 DACELF010000000
VERSION     DACELF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.042"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW687_002893"
     CDS             289..564
                     /locus_tag="IW687_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952089.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW687_002894"
     CDS             577..2655
                     /locus_tag="IW687_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0952090.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW687_002895"
     CDS             2815..4701
                     /locus_tag="IW687_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0952091.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW687_002896"
     CDS             4713..5660
                     /locus_tag="IW687_002896"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0952092.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6206
                     /locus_tag="IW687_002897"
                     /pseudo
     CDS             5680..6206
                     /locus_tag="IW687_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            complement(6269..6922)
                     /locus_tag="IW687_002898"
     CDS             complement(6269..6922)
                     /locus_tag="IW687_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0952093.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7054..7896
                     /locus_tag="IW687_002899"
     CDS             7054..7896
                     /locus_tag="IW687_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0952094.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8054..8950
                     /locus_tag="IW687_002900"
     CDS             8054..8950
                     /locus_tag="IW687_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0952095.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8953..9666
                     /locus_tag="IW687_002901"
     CDS             8953..9666
                     /locus_tag="IW687_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0952096.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9686..10204
                     /gene="msrA"
                     /locus_tag="IW687_002902"
     CDS             9686..10204
                     /gene="msrA"
                     /locus_tag="IW687_002902"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0952097.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10201..10428
                     /locus_tag="IW687_002903"
     CDS             10201..10428
                     /locus_tag="IW687_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0952098.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10580..11131
                     /gene="lepB"
                     /locus_tag="IW687_002904"
     CDS             10580..11131
                     /gene="lepB"
                     /locus_tag="IW687_002904"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0952099.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11214..11756)
                     /locus_tag="IW687_002905"
     CDS             complement(11214..11756)
                     /locus_tag="IW687_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0952100.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11993..12391)
                     /locus_tag="IW687_002906"
     CDS             complement(11993..12391)
                     /locus_tag="IW687_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0952101.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12651..13511
                     /gene="ylqF"
                     /locus_tag="IW687_002907"
     CDS             12651..13511
                     /gene="ylqF"
                     /locus_tag="IW687_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0952102.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13504..14271
                     /locus_tag="IW687_002908"
     CDS             13504..14271
                     /locus_tag="IW687_002908"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0952103.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14327..15184
                     /gene="dprA"
                     /locus_tag="IW687_002909"
     CDS             14327..15184
                     /gene="dprA"
                     /locus_tag="IW687_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0952104.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15306..17384
                     /gene="topA"
                     /locus_tag="IW687_002910"
     CDS             15306..17384
                     /gene="topA"
                     /locus_tag="IW687_002910"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0952105.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17353..18729
                     /gene="trmFO"
                     /locus_tag="IW687_002911"
     CDS             17353..18729
                     /gene="trmFO"
                     /locus_tag="IW687_002911"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0952106.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18927..19832
                     /gene="xerC"
                     /locus_tag="IW687_002912"
     CDS             18927..19832
                     /gene="xerC"
                     /locus_tag="IW687_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0952107.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19869..20417
                     /gene="hslV"
                     /locus_tag="IW687_002913"
     CDS             19869..20417
                     /gene="hslV"
                     /locus_tag="IW687_002913"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0952108.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20431..21831
                     /gene="hslU"
                     /locus_tag="IW687_002914"
     CDS             20431..21831
                     /gene="hslU"
                     /locus_tag="IW687_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0952109.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21849..22640
                     /gene="codY"
                     /locus_tag="IW687_002915"
     CDS             21849..22640
                     /gene="codY"
                     /locus_tag="IW687_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0952110.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22722..23597
                     /locus_tag="IW687_002916"
     CDS             22722..23597
                     /locus_tag="IW687_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0952111.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8211 a         4091 c         4999 g         6426 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaacattcg
     5821 aagggatggg tagccgtccg ttgccgaatc gtcagacgat tgttttgacg agagattctt
     5881 cctataaagc agaaggcgtg catgtcatgc atagtgtgga agacgtgctt gctctggaaa
     5941 aagaaacaga cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc
     6001 cattttgtac gatattatac cgtacagtca tccatcatgt attcgatgga gatgcttatt
     6061 ttccacctgt tgactggtct gattggtcac tgatcaatac tagtcaagga gaaatggatg
     6121 aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa
     6181 gcgggacagg cgaattgacg atataaaaaa gctatggata gactagctgt tatttagagc
     6241 tgtcccatcc atagctttat tttttttatt aagtaaagaa taaaacagag aagtacataa
     6301 aacctgcacc taacataaca aacaaatgcc aaacaacatg catatagcgg atagaattca
     6361 agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc aaaataacac
     6421 cagtcattcc aagagagtga taaagaggaa cagcagccac gacacagagc caccccataa
     6481 aaatatagat gatcgtagat acttttgata ctctctcccg tttatgcaac gtcaacgctt
     6541 tatatataat gccaagaatc gcaaacaacc agattaatac aaataaagtc caacctagcc
     6601 atcctcggat actcagcaaa caaaaaggag tataacttcc tgcaatcaaa agaaaaatcg
     6661 aactatgatc gaatacttgg aacacctttt ttgcttttgt aaaaatcaat gaatgaaaca
     6721 aagtagacgt taaaaataaa agaatcatca tcgatccata gatagcataa gagacaatat
     6781 ggagggagga atgcaagtgg gcacctttta ctaaaagaac cactagtccc gcgatactca
     6841 atccagtccc aattccatgg gtgactgcat taaacacttc atttaatatt tcatacgttc
     6901 gattaaattt cgttttttcc atgaaaacgg ctcctttact aggtgtaatt aatgaaaaat
     6961 cattatttgt ctgttatact aaaaatagaa attgtcttac atgtagttta acatgaataa
     7021 ggcatcttgt taaagaaaga gagagagaaa ataatgacaa atgtaaaaat cgtaacagat
     7081 tcttcatgta cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt catgccgcta
     7141 tccatcatga tcgacggaac agtatatccc gatgatgacc aactagacgg cgaaagattt
     7201 atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc aattggagaa
     7261 ttcatctcga tgtatgatga gctaggagaa gacggcagtg aagtgatttc gatccatatg
     7321 acaaaaggct tgagtggaac agtagaagca gcaagacaag ccagtaatct aacaaaaacg
     7381 aaagtaacgg ttatcgacag tgataccact gaccaaggat tatcttttca ggtaattcga
     7441 gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa tccttgaaga agtcaaaaaa
     7501 gtacaagaca atacaaaatt atacatcgga atatccacac tagataactt ggtaaaaggt
     7561 ggaagaatca gccgaacaac tggtttgctg tctagtgttt tgaacatacg cgtggtgatg
     7621 aatttcgccc attctgaatt aatccctgta actaaggggc gaggaaagaa aacgttcgat
     7681 aaatggttta ataatttgaa aaatgaactg aaagaattgc caaatgtgcg acaaatcggt
     7741 atttcacatg cggatgcaaa agaattggcg caagaattca aagagggttt gcaagaatta
     7801 ttcccggaca tgcatatccc tgtactgcat acgaatccgg ttatcgctac gcatactggt
     7861 aaagacgcgt ttgcaatcat gtattatgta gattagtcga aaagaggata agccctcagc
     7921 ctgaaaggct tgcagcttat tctctttttc tttataaaat taggaaaata attgttttct
     7981 tttttcataa agactacctt tttgataaaa tttttgataa cattagaacc aatcgaatac
     8041 aagaggtgaa aaaatgaaat taacgaaacg acatttgatc ctcgttttgt tcccattatt
     8101 aattgcgatc atcatctatg caggtttagc tgcttctata ccgaaagctg gcccactaat
     8161 gaaaccagta actactgtga gagaagatcc aaataaaaaa gaaaaacttc actacatagc
     8221 tatcggagat tcgctgactg aaggagtagg agatcaaacc aagcaaggag gattcgttcc
     8281 ccttgtcgca aatgatctaa aagaccgtta taatttgacc gcggttgaaa cagaaaacta
     8341 tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga gtaaaaacaa aagaagaaat
     8401 tcgaaacaat atcaaaacag ctgattttat cacactgact gttggcggta atgatttgat
     8461 gaaagttatc caaaataatt tattcgggtt aacggtcaat tcctttaaaa agccactgaa
     8521 aaactatcag gaaaatgtca cagaactatt ggaagaattt cgatccctca ataaaaccgc
     8581 acctatttac attttaggca tctacaatcc attttatttg aactttcctg agatcactga
     8641 catgcagaca attgttaaca attggaacaa aggaacagaa acggtgacaa aaaaaatgcc
     8701 gaactgctac tttatcccaa tcaatgattt gctttatcaa ggattgaatc aggaagtagg
     8761 tgtcacagaa gaaggaacag atcaaacaac tagcacgaca gaaaatctaa atggcatcaa
     8821 aaataatgtg ctctatgaag aagatcattt ccatccaaat aatttaggtt atcaattgat
     8881 ggcaaatgca gtgagagatc agctgatcga gacacaaaac caatggttag agaaggagga
     8941 tgatcaataa acatgaaaac aagatctgac gtatcttcaa acaaaacaaa gaaaacaaat
     9001 tactggaaat tcgccttcct tatccttctt ggggtagtac ttggttcgac cgtattttta
     9061 ggcagccgta tttttgctaa tcgggaacca gaactcccag aaataccagc tcttacggaa
     9121 agacaaggag atcctgtctt aacgatcaat tcaaataaag aaaaagtcaa tcaaatcatc
     9181 agctttttcc ttagtgagta tcaaaaagat agtgatattg aatataaatt ttatttggaa
     9241 aatgaagcac tactcaatgg tactttcgaa gtactaggtt ttcctatcga tttctattta
     9301 tattttgatc cgtatgtgat ggaaaacgga aatgtccaac taaaagctag aagtctttct
     9361 atcggaacat tgagtttgcc aatcaaagat gtgatgaata tgatcaagag aaactataaa
     9421 ttaccagatt ggatcgaagt caatacagat gatctgacta tcatgcttag gttggatcaa
     9481 ttccgaatgc aaaacgggat gtatattaaa gcagataaaa tcgacttagt aaatgatgac
     9541 attcggttta gtctatattt gccaaaagat acctctgatg cttcaaacaa ctcaaatacc
     9601 aacaaaacta gcaccagcag tactgaaagc aagacaaaag aaaataaaaa atcatcggat
     9661 gagtagtatt agagggggaa tcataatgga acgagcaata tttgcaggag gctgcttctg
     9721 gtgtatgatc cagcctttcg acacgcttcc tggtatccat acagtgatgt caggatatac
     9781 tggagggcat gtaccagatc cgacttatga acaagtaaaa agccaaacga ctggacatac
     9841 agaagcagtg gagattatct atgatccaac attgatttca tatgaacaat tagtagaact
     9901 ttactggcag caaactgatc caactgacgc tttcggacaa ttcgaggatc gcggtgacaa
     9961 ttatcgcccg gtgatctttt atttgacaga agaacagcgg atcattgctg aaaaaagtaa
    10021 gaaacagtta gctgaaagtg gacgtttcga acagccaatc gttacaaaaa tcgaaccagc
    10081 ccaaccattt tatctagcag aagaagacca tcaagagtat tataaaaagc atcctgaaaa
    10141 ttttaaaaga aaccatgcac gccgggctgc ctttatcgaa gaaaattgga ggaacgaagg
    10201 gtgaatcgaa gcttttttca ttatttaatg acactgcgag gcggtggcac acatgatgct
    10261 ttatctgttt tcgctacaga agccggtaaa gacatccaat ttcctaagca cagctctagc
    10321 tacgaagaaa tttctaatta tctggaactg aatgtcgact atttgccgtc catggatatc
    10381 tttgacgaag catgggaaaa atatttagaa aataatcatg acatataaga gatagttttg
    10441 tactttatga tcaaaaaagt tttttgtcca atagcctaat tgaaagaatt atttttattt
    10501 cagctatcta gtcgcatttt tttgatattt ttcttataat aaagaaagaa tgaaaaaaag
    10561 agggatggat tgatagagat tgacaaaaaa acaacgctat attaatcgct tctggcttgt
    10621 attcaaatac ttacttgttt ctgtattcct tgcgttcatg ttgcggggat ttttattcat
    10681 cccggtacca gtggaaggca attcaatgga aaacgtgttg aagcaaggtg atatggttgt
    10741 gatggaaaaa ttttcggaga tcagacgatt tgatattgtt gtttttcaat tagcagatgg
    10801 cacgatttat atcaaacgag taatcggtct tccaggagaa aatgtcagct accaaaatga
    10861 tcaattgaag atcaatggga aagtagttaa ggaaccttat ctaactaaaa acctgaaatc
    10921 agatcatgca aatgcatcct atacgacgga ctttacacta caagagctaa cgggacaatc
    10981 caagcttccg gaagataatt attttgtact tggagataat cgaagagttt caaaagatag
    11041 tcgttcgttt ggcacgatca ataaaactga tattctaggt aaagcaagat ttgtctatta
    11101 tccacttgat gaaataaaat ggatccagtg agaaacaata aagcgtgcag tctgttgaga
    11161 ctgcacgctt tattgtttaa tccaaaatca gtttactacc ttagtacgta aaatcaaaca
    11221 gctcgtttaa acacttttct agcctctaaa gttttgataa atgaataaac aaccattatt
    11281 tgaataggga tttggatcat attcctgatg aatctaccta gaaaaattac tttgaaatct
    11341 gcaccaaaca aaacagacat cagccaagaa ttgataaaaa ggctgactgg gatcataatc
    11401 aataattggg cagcgatgac ggaaggcagg tttatctttt tatgatataa aaataagcca
    11461 ttaataaatc caccaaggaa agccgtgata agaaaagcag cttgaaacgt tcctgaacca
    11521 aatatcgtga aatttaaagt gttcgacaac atttcaagca aaccagccca gatcggtcca
    11581 tatagataac ccataagcgc catacttaaa aatccgaatg tgatttgaaa ataagcagga
    11641 ccaatcgcta tcttgtataa gatgacatca agggctaata aaagagcaga ggtcgacaac
    11701 aatcttgcat tcatttcggg caatctaaaa acgtcttttg caatcaaagt gttcaaaata
    11761 ttctctcctt tgtcagttaa taataaaagg ataccataaa atcaccaatg tgtcatgaca
    11821 cattggtgat ttacatacac tggcatctaa caattaaaaa aagggtgaga agactgcttt
    11881 tataaactgt aaaaaagaga ttgtacgaat aaaaaaaata gaggcggaga atacttatcg
    11941 tatcatccgc ctttgttttt ttgttataaa aaacgtcaga ttagttatct gcttacatct
    12001 tcatccatac gggttctaat gtatttccgt ctagatcttg gacttctaat gaaaacattt
    12061 cttcttcagg cattcccata tctacttcgt ggaaagtacc accattttct tttgcaattt
    12121 gagcaaattt ttttacttca tccgcatttt caacactaaa cgaaatcaaa gcaccacttg
    12181 tttttttatt atcagctatt tctttatctt ttataaattt tttataaaaa tcatgagaga
    12241 gaagcattat ccaaaaagtg tcatcccata ccattgaacc agcttcttca gtagaaaatt
    12301 cttcattttt tttaaaaccc agcttttcat aaaaagcaac ggaacgattg acatcagcaa
    12361 caggaaaatt aacaaaaacc atactattca tttgtttcgt ctcctttctg ttttgttcgt
    12421 ctatcatctc ttatttagga aacgctgtca aaaatttaga ttcatatata gaaaccaaaa
    12481 taaaaatttg catcatcaaa aaagtgaaga tgtgatagaa tgaggactgg ttcactttca
    12541 cttaactaat agatggaatt tctttataat aaaaaagtga atttatattg aaaaagaaaa
    12601 aaacaagtaa agccggggaa tattccggta tgaaaggaat ggagagaaca atgaccatcc
    12661 aatggtttcc gggacatatg gcaaaagccc gacgagaagt ttctgaaaaa atgaaatatg
    12721 tggacatcgt atttgaattg gtagacgcac gattaccttt atcttctcgt aatccaatgc
    12781 tagatcagat cattcagcaa aaacctcgcc ttgttttatt gaataaagcg gatctggcag
    12841 atccacaaca gacaaagctg tggcaagaat attttcgtaa acaaggccat cttgcattag
    12901 ctatcacggc acaagaaagt aaaggaatta aaggtctagt acctgcggct aaagaagcat
    12961 tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa gcctcgcgct attcgggcaa
    13021 tggtcttagg tattccaaat gttgggaaat ctacgttgat gaatcgacta gtaggcaaga
    13081 aaattgcaca gacaggaaat aagcctggcg taacaaaagg tcagcagtgg cttcgtttag
    13141 gtaatgagtt ggaattgtta gacacaccag ggattttatg gcccaaattt gaagatgaag
    13201 aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga ccagcttgtc catttagatg
    13261 atttagcgat ttacggatta gacttttttg cccgatatta ttcaggaaga atcagcgatc
    13321 gttatggttt atcaatcgag caagaagaaa aattggcacc ggaattactg atggatatta
    13381 cagcaaaacg tgggtataaa gaagactatg atcgtggcag cgaattagtc gtgcatgata
    13441 ttcgtcaagg aaaacttgga cgatacacac tggatcgttg cgaagaaatg gagcaaaaaa
    13501 gtgatgactg aatccatcca aagtatcaaa gaacgattga aaagcataca gagaatagat
    13561 gatcctacac tagaatcatt aagaaatgat ccacgcaaag gcgtgcagca agcactggct
    13621 tcttttgaac gacgattgaa aaaggaagca ttaattaagg aaaaatatga agaaatgttt
    13681 tgctttgaaa agcaagggta cgcacaaggt caccaatgga ttgccggcat tgatgaagta
    13741 ggaagaggac cattagctgg acctgtcgtg gcagctgcag ttatcttgcc gaaaaatgag
    13801 atcatattag ggctgaatga ctccaaacaa ttatcagaga aaaagcgaga acttctttat
    13861 caacaagttc aagaaaaagc cctggcaatc gggatcggga tcgttgatca gcagacaatc
    13921 gatgatgtga atatttatga agcaagtaaa ttagcgatga tccaagcagt agaaaaattg
    13981 tccattgtac caagctattt gttgatcgat gcgatgacct tggatttgcc tattcagcag
    14041 gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg ctgctgcaag tatcattgct
    14101 aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc tttatcctgg atatggtttc
    14161 tcgcataatg caggttatgg aacaaaggaa cacttagaag gacttgaacg acaaggaatt
    14221 acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat atttacaata aaaaacagcc
    14281 gcttttacgt atcttattag aaagagagac gaaaggcggt tttatgatgt atcaaataga
    14341 agaaaattta ttgaaacttg cttatacaaa aggaatcagt agtcaaggaa aatgggcact
    14401 tgctaactgg atgatgcagt atccttataa agaaatcgga tttgaagaca tcattaagat
    14461 aggtaaaata accaaacacc gggagctgtt tcgcaaaagt tggcaggaaa ttcacgctaa
    14521 ctggggcaaa atccaatcga aacagtcgtt tatcacttgc tttgatccca aatatccccc
    14581 tcagctgctt cacttgacct atccgccaat tgtactgttt tataatggtg atctatcttt
    14641 actttcttgt aacatgttaa gcgttgttgg tggaagacac acttctgctt tagccaaaaa
    14701 aacagtttat tatttactaa aaccagtgat agaagctgga tatgtgatcg tcagtggctg
    14761 cgcgaaaggg atagatacat acggtcacca agcagcaatc tctcatggtg gacggacaat
    14821 cgcagtcatt ggaacaggga tagagcaagt ctatcctgct gaaaatcgtg acttgcaaga
    14881 ggtgattgca aatgatcatc ttcttttgag tgaatatcct cctcaatccg gcccgaaaaa
    14941 gcattgtttt ccaatgagaa atcggattat cgcagctttg tctaaaggaa catgtgtctt
    15001 tgaagccaag aaaaaaagcg gctctctgat aacagcacag caagcattgg atcttggaag
    15061 aacagttttt tctgttccag gaaatatttt aacagaacat tctaccggat gtcatcattt
    15121 gatccaagac ggtgcagttt gcacggtttc gggtgaagat atcttggcag aatttaaaga
    15181 ataacaccat acttttcatt tgcttttctt gacaaacgtt ttctgaatgg atatgattag
    15241 ctacgatttg tatgaatcct cttttaatag taggaacacg acaatggaaa ggagtccatt
    15301 cagtaatggc gtataaatat ttagtgattg tagaatcacc tgcaaaagcc aagacaattg
    15361 agaaatatct tggacgaaac tataaagtaa tggccagtgt tggccatata agagatttac
    15421 cgaaaagtaa aatgggtatc gattttgaaa acaattatga accccattat atttctatac
    15481 gcggaaaagg cgatgtcatc aaaagcctga aagccgcagc aaaaaaagct caaaaagttt
    15541 acttggcaag tgacccggat agagaaggag aagcgattgc ttggcattta gcgtacctac
    15601 ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa tgaaatcaca aaagacgcag
    15661 tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga tttagtagat gcacagcaag
    15721 ctcgtcgtac cttagacaga atcgttggtt attcgatcag tcctattctc tggcgtaagg
    15781 tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc tttaaaaatt attattgacc
    15841 gtgaaaaaga gatccgagaa tttgttccag aagaatattg gagcatcgac ggtaatttta
    15901 aaaaagctcg caagaaattc aaagcaaatt tctggggaat cgacggtaag aaaaagaaat
    15961 taccagatgc acaaagtgta aaagaagtca ctgctagaat tgatggaaca gacttcgaag
    16021 ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc gaatcctttc actacaagta
    16081 gcctgcaaca agaagctgcc cgtaagttaa actttcgaac aagaaaaacc atgatggtag
    16141 cacagcaatt atatgaagga atcactttgg gtaaacaagg aacagtcggc ttgattacat
    16201 acatgcgtac tgattctacc cggattgccg atacagcaaa agcagaggcc gcagcattta
    16261 ttgaagatac gtatggaaaa gaattttcta gacatgatca ccgaaagatc aaaaatgctc
    16321 aaggcgcaca agatgcccat gaagctgtgc gtccaacgag tgtgcttcgt acaccagatg
    16381 aactcaaaca atatttagat aaagatcaat tgaaacttta tacattgatc tggtcacgtt
    16441 ttgtggctag tcagatgaca ccagctattt tagatacaat gaaagtgaca ttagaacaaa
    16501 atggtgtcat cttcattgcg aacggttcaa aaatcaaatt caaaggattc atgcaagtgt
    16561 atgtggaagg tagagatgac ggcaaagaag agaaagaaaa tattcttcca gaacttgaag
    16621 aaggcgatgt agtccaatct gttgatattg aaccaaaaca acattttact caaccaccag
    16681 cacgttttag tgaggctacg ttgattcgtg cactagaaga aaacggggta gggcgacctt
    16741 ccacttatgc accaacactt gatacgatcc aaagacgcta ctatgtaaaa ttgacacaaa
    16801 aaagatttga gccgactgaa ctaggagaaa ttgtcaattc attaatctgt gaattcttcc
    16861 ctcaaatcgt cgatatccat ttcacagcag aaatggaagg agatttggat aaaattgaag
    16921 aaggcacgga agcgtgggtc aaagtggttg atcgtttcta taaaccgttt gaaaaagaac
    16981 tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa agatgaacca gcaggattcg
    17041 attgtgatgt ctgtggacat ccgatggtga taaaattagg gaaatatggg aagttttatg
    17101 cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat cgtaaaagaa atcggtgtga
    17161 cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa atctaagaaa aacagactat
    17221 tctatggttg ttcccgtttc ccagaatgtg aattcacttc ttgggataaa ccagtgggca
    17281 gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa agtcaaaggc ggtaaacaag
    17341 tagtctgtgt aaatggagat tacgaagaag acatccaaaa ataatagaaa agaggttctt
    17401 tcattgaaaa tggtaaatgt tgttggagca gggcttgccg gaagtgaagc tgcttggcag
    17461 atcgcacaag caggagtccc tgtccgttta tatgaaatgc gtccagtcaa atcgactgag
    17521 gcgcatcaca caagtaattt tgctgaacta gtatgttcca attcattaag aggaaacagt
    17581 ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc gattgaattc ggtagtaatc
    17641 acttcggcag atgaaacagc agtgccagct ggtggtgcct tagctgttga ccgcgattct
    17701 ttttctgaaa caattacccg aaaagtcaaa gagcatccat tagtaacagt gataaacgaa
    17761 gaaattactg aaataccgga aggaatcacc gtagtagcaa caggaccttt gacttctcat
    17821 ccattagcag aaagcatcaa agcattcaat ggttcagatg gtttttattt ctatgatgca
    17881 gctgcaccaa tcgtagacaa agctacgatc gatatggata aagtatattt gaagtcccgc
    17941 tatgacaaag gagaagcagc ttacttgaac tgtccgatga cagaagagga attcaaagct
    18001 ttccatgagg cactaatcaa tgccgaagtg gcagaactta aatcttttga gaaagaaaaa
    18061 tattttgaag gatgtatgcc aattgaagta atggcacagc gaggattcaa aacgatgctt
    18121 ttcggaccta tgaaaccagt tggattggaa gatcctaaaa ctggcaaacg tccttatgct
    18181 gttatccagc tgcgtcaaga caatgctgca gcttctttat acaacatcgt gggctttcag
    18241 actcacttga aatggggcga gcaaaaacgt gttttccgga tgattcctgg gttagaaaat
    18301 gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct ttatgaattc gccagaattg
    18361 ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt tctttgcagg tcagatgact
    18421 ggtgtagaag gctatgttga aagtgcagcg agcggactac ttgctggaat caacgccgca
    18481 cgcttagcaa aagaaatgga accagtcgag tttccacaag aaacagctat tggaagtatg
    18541 gcttactaca ttactcatgc ggaaggaaaa catttccaac cgatgaatgc caactatggt
    18601 ctgtttcctg aactgccaga gcgcattcga gacaaaaaag caagatatga agcaattgca
    18661 caacgagctt taaaagctaa tgaggcaata aaaacagaat tagaaaaaac tgcaatccca
    18721 aatgagtaag gaaactccat gaagagggtg agacggatag tctcgctctt tttcttgcgt
    18781 ttttcttaag aatttctcaa acaaaatgtg aggaattgcg ccaattgtga acaaacttag
    18841 aaatattggt taaattctga caatttattt gtctttttat aaacgctgtg ctaaattatt
    18901 ctctgggaca aaaggaaggt gagaaaatga cagaagccac gtgggaacaa aaatttttgc
    18961 gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa agaagcatat gaagaagaca
    19021 ttctccattt caaacatttt ttggaggaat ctggagacgc tgatctgctg aaggcggaat
    19081 attttacggt tcgtgtctat ttgagtgcat tagctgatca tcgtcctagc tatagcagaa
    19141 attctatcag tcgcaagata gccagcctac gttcgtttta tcagtatctt ctcaaagaag
    19201 aagtgataaa agaaaatcca ttttcttacg ttcatctcaa aaagaaaaat ttgagactgc
    19261 cacgtttttt ttatgaaaat gaaatgcaag cactttttga cagcgtagca ggagatagtc
    19321 ctttggattt gaggaaccgc gctttactgg aagtccttta cgggtcaggc atccgtttaa
    19381 gtgagtgtag cagtttgtcg gtatcagaca ttgattttga ttcagaggtc atgttgatcc
    19441 atggtaaagg gaacaaagag agatatgctc ctctaggttc ttttgctcaa gatgctctgc
    19501 aagaatattt cagggaaggc cgccaggtat tgatgacaaa atatcacgaa gagcatgata
    19561 tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac tggaattgaa tatgtcttga
    19621 atcaaatgat caaaaaaagc agtttggata gtgatatcca tcctcacatg ctgcgccata
    19681 catttgctac tcatttatta aataatgggg cagatttgcg gacagtccaa gaattgctag
    19741 gacatgccaa tttatcgact acccagatct atgcccatgt aacaaaagaa agtttacaaa
    19801 aaaactatcg ttctttccat ccaagagcgt aaaaataagg aaaagaacaa atttaggagg
    19861 aaacaaacat ggtcgaatca ccatttcatt caaccactat ctgtgccgtc gaaaaagacg
    19921 ggaaatttgc tatggctgga gatggccaag taacaatggg tgaatctgtt gtcatgaaag
    19981 gcactgcaaa gaaagtacga cgtatctaca atggagaagt tgttgtaggt ttcgccggaa
    20041 gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg caaactaaac gaatataacg
    20101 ggaatctaca acgcgcagct gtcgaattag ctcaagaatg gcggacacag caatctatgc
    20161 aaaaacttga agcgatgctg atcgtaatga atgataaaga aatgcttctt gtttcaggta
    20221 caggagaagt tatcacacca gatgatggta tcttagctat cggatcagga gggaattttg
    20281 cgttggctgc agcccgagca atgaaaaact atgctcaaaa cgacatgagt gcgaaagaaa
    20341 ttgcagaaaa tgcgttaaat attgccgcag atatctgtat ctttactaat cataatatta
    20401 tcgtagaaga actgtaagag gtgagtagat atgtatgaat taaaacaaac accaagacaa
    20461 atcgtaagtg aattagatga atatatcgta ggacagcacg cagcaaaaaa atctgttgcc
    20521 gttgctttgc gtaaccgcta tcgccgtttg cagttagatg aaaaaatgca acaagatatt
    20581 acaccaaaaa atatgctgat gatcggacct acaggggtcg gtaaaacaga aatcgcacga
    20641 cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg aagcaacaaa atttactgaa
    20701 gtcggttatg tcggaagaga tgtagaatca atggttcggg atttagtaga aaatgcgatc
    20761 caaatcgtag aaaaagagca gtacagccgt gtatacacaa gtgctaaaaa aaatgcagaa
    20821 aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag aacagaaaca atccactaat
    20881 caatttgaac aaatgatgaa tctttttaat gctagtcagc aaaataccgc tgaaccgcaa
    20941 gaagaagtga cagaagatat tcgaatcaat cgccgtacag tcttggaaca actacaaaag
    21001 ggtttactgg atagtcgtga agtaaccatt gaagttgaag accaaaagaa acctgcacca
    21061 atgatgaaca atggattgga gcaaatgggg atcgacctaa atgaaactct aggtgctttg
    21121 acaccgaaga aaaaagtgga acggacggta acagtcaaag aagctttaga gttattgatc
    21181 aaggaagaat cagcaaaatt agtcaaggat gctgatatcc atagtgaggc aatcaaatta
    21241 gcggaagaaa gtggaattat ttttatcgat gaaatcgata agatcacttc taaatcacag
    21301 caatctggag aagtatccag agaaggcgtc caaagagaca tattgccaat tgtcgaaggt
    21361 tcacaagtaa acacaaaata cgggatcatc caaacagatc acgtcctatt cattgcttca
    21421 ggagctttcc atttgtctaa accaagtgat ctaattccag aacttcaagg acgcttcccg
    21481 atacgggtag aattagatga tttgacagct gaagattttg tgaagattct gaccgaacca
    21541 aataatgctt tagtaaaaca atacgtcgcg ttgatcggta cagaaaatgt gtcagttacg
    21601 tttacaaaag aagcaattga acggatcgct caaatcgctt tcgatgtcaa ccgtgacaca
    21661 gataatatcg gcgcacgcag actgcatacc attttggaaa aactactaga agatctatta
    21721 tttgaagcac cagatatgca aatgggagaa ataacgatca cagaagcata tgtggatgac
    21781 aaacttggct caattgcggc aaatgaagat ttaagtcgct atattttata aaaaatcgga
    21841 ggacgacaat gacagacttg ctaacgaaaa cccgcaagat aaacacactt ttgcagcaaa
    21901 agaatacttt tgacatgcaa gcagatttgc cttacgataa aatggctgtc acactaggtg
    21961 gtattcttga cagcaacacc tatatcatag gaagtgacgg agtattacta ggttataatg
    22021 aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt tgttgagaaa caatttcctg
    22081 aatcttatac tgatacagtg gcacagttga tgaaaactga agctaatatc ccaattacta
    22141 gtgatattac tgcatttccg gtagagtcaa gagagaaata tccatttggt ctgacgactg
    22201 tcgttccgat ctttggagct ggcgaacgtt tgggtacgat tatcttatct cgtatggaac
    22261 aatcgtttgc agatgacgat ctagtcttag cagaatatgg agctacggtt gtcggtatgc
    22321 agatccttta tcagaagtct cgaagcatcg aagcagatgt acggagtgca acggctgtcc
    22381 aaatggcaat caacacatta tcttatagcg aattgaaggc tgtacaagct atctttgaag
    22441 cattggatgg agatgaaggt cgtctgactg cttctaatat tgccgatagt atcggcatca
    22501 ctcgttctgt gatcgtcaat gctttacgaa aattggaatc agctggaatc attgaatctc
    22561 gttcgttagg catgaaggga acttatttaa aagtattgaa ttcccgtttt aaagacgagt
    22621 tagcaaaaca cagttattag aagtaagggg actggggcag atagaaatgt ttgttccagc
    22681 cttcccattt taaaaagtaa gtcgaagttg gaggaatggc aatgactgtt attattcaaa
    22741 atgaccagct gatcgccgaa atcgctgaac acggtgcgga attgatcagt ctgaaatcaa
    22801 aagaacatgg tttagagtat atctggcaag gtgatccagc gtattggggc agacatgcac
    22861 cggttttatt cccttttgtt ggacgtttaa aagatgatca gtatgtttat caagggaaaa
    22921 catatcaaat ggggcaacac ggatttgctc gggatatgga ttttgaagtc attgaacatg
    22981 gacaagaaat ggctagtttc atcttaagga gtacgccgga gacattagaa aagtatccat
    23041 ttgattttga attagtgatc agctatgaat taggaggaga cggcatcacg gttcattatc
    23101 aagtggcaaa taccggtgaa aaagaaatgt tcttttctat cggtggacat cctgctttca
    23161 atgtacctct tgaagaagga ttgtcatttg aagatttcta tctggcattt tctccattga
    23221 aatcaagatt acgcctacca ttgaaaggcc cattcatcga tatggatcaa aagacgttag
    23281 gtcagacaaa tacgaatctg gctttgaatc gtgaactttt tgagcaagat gccttggttt
    23341 ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga aaaatcttcc catagcgtaa
    23401 ctttaagcta taaagatatg ccttatgtag ggatctggtc tccttatcca aaagaagcgc
    23461 ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac ggtgaacagt agtggaaact
    23521 tgatggaaaa agtagggatc aatcaactag gtgcccatga aatcttcaag acaaaatatt
    23581 ccatcacagt gaagtaagag gttgtgaccg agaagttagc agggaacacc gtttaccaag
    23641 gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg acatcgattt gtggctcttt
    23701 gtcaataagg actgatgagt tgtgcaa
//
LOCUS       DACELF010000274        20863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.048, whole genome shotgun sequence.
ACCESSION   DACELF010000274 DACELF010000000
VERSION     DACELF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.048"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            267..1304
                     /locus_tag="IW687_002917"
     CDS             267..1304
                     /locus_tag="IW687_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0952112.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IW687_002918"
     CDS             complement(1393..1716)
                     /locus_tag="IW687_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0952113.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IW687_002919"
     CDS             complement(1703..2410)
                     /locus_tag="IW687_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0952114.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IW687_002920"
     CDS             2496..2837
                     /locus_tag="IW687_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0952115.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IW687_002921"
     CDS             complement(3008..3856)
                     /locus_tag="IW687_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952116.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IW687_002922"
     CDS             4454..5317
                     /locus_tag="IW687_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0952117.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IW687_002923"
     CDS             5417..5899
                     /locus_tag="IW687_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0952118.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IW687_002924"
     CDS             complement(6162..6629)
                     /locus_tag="IW687_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0952119.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IW687_002925"
     CDS             6752..7603
                     /locus_tag="IW687_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0952120.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IW687_002926"
     CDS             complement(7854..9812)
                     /locus_tag="IW687_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0952121.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IW687_002927"
     CDS             complement(9809..10681)
                     /locus_tag="IW687_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0952122.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IW687_002928"
     CDS             complement(11023..11895)
                     /locus_tag="IW687_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0952123.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IW687_002929"
     CDS             12023..12592
                     /locus_tag="IW687_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0952124.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IW687_002930"
     CDS             12659..13396
                     /locus_tag="IW687_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0952125.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IW687_002931"
     CDS             13627..14895
                     /locus_tag="IW687_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0952126.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IW687_002932"
     CDS             15382..15846
                     /locus_tag="IW687_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0952127.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IW687_002933"
     CDS             15830..16459
                     /locus_tag="IW687_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0952128.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IW687_002934"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IW687_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0952129.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IW687_002935"
     CDS             18338..18784
                     /locus_tag="IW687_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0952130.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IW687_002936"
     CDS             complement(18983..19498)
                     /locus_tag="IW687_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0952131.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="IW687_002937"
     CDS             19615..20817
                     /locus_tag="IW687_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0952132.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACELF010000275        11130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.086, whole genome shotgun sequence.
ACCESSION   DACELF010000275 DACELF010000000
VERSION     DACELF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.086"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            118..1620
                     /locus_tag="IW687_002938"
     CDS             118..1620
                     /locus_tag="IW687_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0952133.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1687..2214
                     /locus_tag="IW687_002939"
     CDS             1687..2214
                     /locus_tag="IW687_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0952134.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2425..3486
                     /locus_tag="IW687_002940"
     CDS             2425..3486
                     /locus_tag="IW687_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0952135.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3489..4385
                     /gene="murQ"
                     /locus_tag="IW687_002941"
     CDS             3489..4385
                     /gene="murQ"
                     /locus_tag="IW687_002941"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0952136.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4399..6381
                     /locus_tag="IW687_002942"
     CDS             4399..6381
                     /locus_tag="IW687_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0952137.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6446..6892)
                     /gene="nrdI"
                     /locus_tag="IW687_002943"
     CDS             complement(6446..6892)
                     /gene="nrdI"
                     /locus_tag="IW687_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0952138.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7103..8527
                     /locus_tag="IW687_002944"
     CDS             7103..8527
                     /locus_tag="IW687_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0952139.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8708..9481)
                     /locus_tag="IW687_002945"
     CDS             complement(8708..9481)
                     /locus_tag="IW687_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0952140.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9478..10383)
                     /locus_tag="IW687_002946"
     CDS             complement(9478..10383)
                     /locus_tag="IW687_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0952141.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(10537..10857)
                     /locus_tag="IW687_002947"
     CDS             complement(10537..10857)
                     /locus_tag="IW687_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0952142.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3561 a         1986 c         2380 g         3203 t
ORIGIN      
        1 tttcgggatt ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc caacccttgc
       61 atcctcttta gccagttctg aaaataggac tggtcttttt ttacgaggtg atggaaaatg
      121 tctaaaatcg aaataaaaaa tctgacattc ggctacgaca gccaaggcac attattattt
      181 gaacaagcaa atctaaattt tgacacacaa tggaaactag gacttatcgg acgaaacggt
      241 cgaggaaaga caactttact gaatattcta caaaacaaac taccttatca agggcaagta
      301 atccatcagc aagaatttgc ctatttcccg caacagacaa aagataaaga acgtttaacc
      361 tattacgtgt taaatgatat tacggatttt gagatatggg aaatcgaaag agagctccaa
      421 ttgatgcaaa cagatcctga aatcttatgg agagaattca gcacactatc ggggggagag
      481 aagacaaaag tcctactggc acttttattt gtggatgaca ctcatttccc gttaatcgat
      541 gaaccaacga atcatttgga tatctctggt agaaaacaag tagcggctta tttgaaaaag
      601 aaaaaacaag gcttcatcgt ggtcagccat gaccggggat ttatcgatga agtagtggac
      661 catgttttag caatcgaaaa aagtcaactg gaactttatc aagggaattt ctctatctat
      721 gaagaacaga aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa
      781 gaagtcagta ggctaaagaa aacagcagct gaaaaagccg aatggtctcg ttcccgagaa
      841 ggagataaaa caaagaaaca agtcggattc atcgatactg aatctagacg agtgaataaa
      901 ggagcagtgg gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg
      961 atggagaccc agatcagcga gaaagaaaaa ctattaaaag atatcgaata tatcgattct
     1021 ttgacgatga atagccaagc gtctcaccat aagcgacttt taagcgtaga agatcttcaa
     1081 ttagggtatg aaaatctgtt attcgagcca attcatttta caatcgagcc tcatcagcgg
     1141 gtggcgattt caggtcctaa cggtgcagga aagtcatcca ttatccatta tcttctgggg
     1201 gcattcaacg gcaaggttat aggagaaaaa taccagccaa aacatctgag cattagttat
     1261 gcaagccaaa attatgaaga caatcgagga acgttggcgg aatttgcaga gaaaaaccaa
     1321 gtagactacc aagcattttt gaacaacctc cgaaagcttg ggatggaaag agatgttttt
     1381 cataacaaga tcgagcagat gagtatgggc caacggaaaa aagtggaatt ggctaaatct
     1441 ttatcacagc cagctgaact atatacatgg gatgaaccat tgaattattt ggatgtcttc
     1501 aatcaagaac aattagaaca actgatcttg aacgtgaaac ctgccatgtt actagtggaa
     1561 catgatcaaa ccttcctgga taaagtatct actgagatta tttctcttga gagaatctaa
     1621 cccttgtaat ctcaagaaca acaaggtatg gttaagggga actttgccaa cgaaggagag
     1681 aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt
     1741 tgcggacggg cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca gaaactagca
     1801 gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga
     1861 gcaagagata cagcacaagc agttgctgat cgtctcagct tgccgctgac cgttgatgaa
     1921 cgattggtag aaatggattt tggggattat gatggactgc caagcaaaga tgagaacttt
     1981 caaaaagcac gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt cttagatgtg
     2041 tatgcacgga tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg
     2101 ttagtttgcc ataatgcctt gataagagtg atcaatgctt attttcatcc gatgcctaat
     2161 gaggggttct ttacattcat ggtagataat acagaattgg tttcttatga gtaattatgt
     2221 ttttgtgtta tttaaataaa atatttcaag gcactgcttt tgcttagcag tgcctttttt
     2281 aggttatgac aattcttttg ataatataaa ctcaaccttt agaaatgaaa tttaattatt
     2341 ttaataaaag tattgaaatt ttgtttcttt agatatatac tctagtcata gaaatgaaga
     2401 cggatacaga aaaagaggga taatatgttt ggcttttcaa tatttatgaa cgattctttg
     2461 tcagaagaga agaaacagta tatccgccaa atggcatcca atggctttgt cggtattttc
     2521 acttccatgc atataccaga agatgatgtt tcggcatatc aaaagcgatt gatcgaacta
     2581 ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg atatttcagg agaggcattg
     2641 gaacgagcgg gtttttcatt agaagatcta acgcctttga aagcaattgg tgtaacaggt
     2701 ttaagaatgg attatcatat ttccaatcag cagattgccg actggtcaca tcaattgaaa
     2761 atcagtttga atgctagcac gatcacgcct aaagatatcg atgaattgaa agaagcagaa
     2821 gctgatttct cacagattga agcttggcat aattattatc cacgtcccga aacagggctg
     2881 gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa
     2941 ggttttgtac caggagatac agagttgaga ggcccactat atcaaggatt gccaactttg
     3001 gaagaacatc gagggatcca tcctcttgca ggcgctctag atctattagc aagtaacacg
     3061 gatattgttt acatcggaga tgcaggtctg tcagaaaacg ttcaggagca atttgcttct
     3121 ttccaaaaag aacagacagt cttgctccat acggaacctg tagatgaaga attttacgaa
     3181 tatatattag ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta
     3241 gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa atacagctac gcgtatgaaa
     3301 gggtctatta ctttagataa cgaaaagtat ttgcgctata tgggtgagat ccaattaacc
     3361 aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat caaagaagag
     3421 ctaccattga tcaaccaaat caaagctggt atgaactatc aatttataag aaaagaagga
     3481 agataaaaat gagcaaaatc aatttagaga acctaacaac ggaacgcaga aaccaagaaa
     3541 cttttggatt agatgagatg agtatttctc aagccgttca actaatgaac aaagaagatc
     3601 agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg attgctaaca
     3661 cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat gggagccgga acaagtggaa
     3721 gactaggtgt gttagatgct gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg
     3781 tcataggtct aattgctggt ggagaagaag caatgactgt agctgtcgaa ggggccgaag
     3841 attcttatga gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa aatgatatag
     3901 ttattggtat cgcagctagc ggtcgtacac catatgtgat cggtggatta gattacgcaa
     3961 aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa acaagccgaa atcagtaaac
     4021 acgcggattt cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc tctactcgat
     4081 tgaagtcagg aacagcacaa aaacttatct tgaatatgat ttctactatc agtatgattg
     4141 gtataggtaa agtattcaat aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa
     4201 tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc tgattacgaa acagctgaaa
     4261 agtactttga agaagcagat caaaatgtta agctggctat cgtcatgatt ttgacaaata
     4321 gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta aaagggacct
     4381 tatgaggggg aaaagaccat ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa
     4441 atttataaag gggcaggcgg catgggcaat gtagaaagca ttgtccactg catgactcgt
     4501 gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa agcgattcca
     4561 ggagtgcttg gtgtagtaga agatgaacag cttcaagtca tcatcggacc tgggaaagtg
     4621 aacaaagtag cacagcaaat ggttgatttg gcaggcgtga aattaggaga aaaactttct
     4681 tctgtaagta cagctacagc aaattcatca gcatctggaa aagaccgtgt aaacgaacgg
     4741 gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat caaatcggta
     4801 ttaaaagaca tttctaatat ctttgtgcca atgattcctg catttgtcgg tactgggatc
     4861 gtggcaggga ttgccgcagt tttagcaaac ttagtcacag ccggaacact tgatgcagca
     4921 acatggcaac aatatatcga tgtgatgaac atcttgaaaa acgggatgtt tagctaccta
     4981 gttatctata caggtatcaa tgctgcacaa gtttttggtg caacaccaac tcttggtgga
     5041 gttatcgggg ccgttgtgat gctgacaggg atgaatccgg aagcaccact gaaaaactta
     5101 tttactggtg aagctcttgc agctggacaa ggtgggatcc taggtgtcat cttcgctgtt
     5161 tggctattgt caatcgttga gaaaaaactg cataaagtag tacctgatgc ggtagatatc
     5221 atcgttacac caactctttc attgattgcc atcggtctga ttgaaatttt cttcatcatg
     5281 cctttagcag gtttcatttc tgatggcatg gtcggtggga tcaactgggt acttggcgtt
     5341 ggtggagcat ttgccggctt cgttctaggc acattattct tgccgatggt tatgttcgga
     5401 ctgcatcaaa ttttgactcc aatccatgtg caaatgattg atgaaactgg tcgtacgcta
     5461 ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg
     5521 attcgctgca aaaaagataa agaattaaca gaaatgatca aaggagcttt accagtcggg
     5581 atcctaggaa tcggcgaacc attgatttac ggagtaacat tacctctagg acgcccattt
     5641 attactgctt gtatcggtgg aggtatcggc ggggcagtga tcggaatgat cggaaatgtc
     5701 ggggctatcg ctattggacc ttcaggagcc gctttgatcc cattgatctc tgacggtaaa
     5761 tggttaggct acatccttgg attattggca gcttatgctg gtggcttcgt tgctacattc
     5821 ttcttcggta ttcctaaaga ccgtttagaa aaagaagaat tagcgactga aacagccaca
     5881 gtaaatgaag tgcctgaaaa agtagtacct caaacttcag ctgaaagtgt gactatcctc
     5941 tcatcagttg ctgacggaac agttgtttct ttagataaag caagtgatcc agtttttgca
     6001 caaaagatga tgggtgaagg ctactttgta gaaccagaaa acggacatat ctattctcca
     6061 gttactgggg tagtcagctc tgtcttccca acgaaacacg ctattgggat cacaacgcca
     6121 aatggtttag aaatcttgct tcatatggga atcaacacag ttgacttagg cggtacgcca
     6181 tttgatgtga aagtaacaga agggcagcaa gtgacacctg atacgcttgt agcagaagct
     6241 gacttagaag cgatcaaagc agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat
     6301 atggatcgtg taaaaaactt tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct
     6361 gtgatggatg tcaaagctta atatgataga aacaatagaa acgaaagaag aaactgaaat
     6421 ctatttttca gcttcttctt ttttattatt tattgttttc tgctaaaatc tgataaattc
     6481 gtgtcacatc agatggtgtt ccccgtaatt cataatctgc caagaaagga aagccaaatg
     6541 tttgtgcata ttgtttggct gttagacaat actgattatt gaagttttta tttccgctac
     6601 cgactacacc aagacaaagt ttatgattgt cttcatgttc cagatattca cgcatcgttt
     6661 cggtcaagat ttctgtatca ccgttatcta aaccatttcc gccgtctaaa taagtaggaa
     6721 cgaaagtaaa aaatggttct gtttctttcg taaaatcact attttcatgg atttctttta
     6781 aagtaacttc aggattgaca ggatcttctg catgcatttt ttcagcatat tctgctagat
     6841 gtttggcaaa ggcacgcgta tttcctgaaa tcgatatata taaaatgttc atggatcatc
     6901 ctccaattct ttccttttag tatagcatct tttttaagtt ttttggtggt gtaatttgga
     6961 aagggactag gcgctttgct tgtataatga gaaaaaagcc ggtagaatta tatttctatt
     7021 aaagttttta ttaattaagt aaaaataaaa aaggtttgtt atactttttg ataaaagcgg
     7081 ttttattttg gaggatgctt acatggatac agagcaacag aaaaaaggaa ttggtttaat
     7141 tccacttgct gccttagtaa ttggttcagc gattggaggt ggcgtatttg gtattatgac
     7201 ggacatctcc ggatcggctg gcggcccagc tattttcagt tgggtactcg taggaacagc
     7261 aatgttgatg ctctccttgt cgatcaataa tatcaataaa aaacgtccag atttaaaagg
     7321 cgggatcttt agttacgcag aagaaggttt tggccgtttc gctggtttta tcagcggttg
     7381 gggttactgg ctcacatgct ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc
     7441 attaggatat ttcttcccag tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag
     7501 tacagtcttt ttatggggat atgcatggct agtgaatcgg ggagtcgaaa atgctagttt
     7561 cgtcaatgct atcgtcacat tctgtaaatt agtgccatta tttttattta ttgtagtagc
     7621 tatcagcacg ttttcgatcc aacaattcat tgataacttt tctagcgaca ttatattgaa
     7681 tgcgaatgga cagccgatcc cgttttccag tcaagtattg agttgcatca tggtgatgct
     7741 ttgggtattt gttggtattg aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc
     7801 agatgttggg aaagcaacga ttctagggat attcggcttg attattattt acgtcttggt
     7861 ttccatgctt ccatatggga caatgacgat gaatcagctg caaacgatca aggcgcagcc
     7921 tgctatgggc tatgtgtttg aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg
     7981 cggtttgatc atttctttag ttggtgtttg gctctcatgg acgatcttac cgattgaaac
     8041 aatgggaaac atggctgaag acggactttt gcctaaagca tggggaaaac tcaacaaaaa
     8101 tggggcacca acttatgcta tcgtattgac gacgttatgt acgaatgtct ttttactttc
     8161 attattattc actgacagtg cttataactt tgcttacaca cttggtacag cagctatttt
     8221 cttcacgtgg cttttcttag gattgtacca aatgaaactt tcttatcagc ggaaagaatg
     8281 ggtccaattt ttagtagggc ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt
     8341 agcctttaat gaagtaatgc ttgttcttgt tcttttcttg ccaggcattt atttttacct
     8401 caaagcacgt aaggagcaaa acgagcctgt acaacatatc cacttagata agattttact
     8461 tataggtgtg acgatggtcg gagtgattgc aatggtttta tttgccactg ggatgcttca
     8521 cgtatagaag tataaaagaa gtataaagag aggttgtgag cctgtcgttt attcaaaaac
     8581 atactaggaa aggagctgtg acacaaatgt tgtcacagct cctttttcag aataaacggt
     8641 gtttaagaga cagccaatcg gcaataaggc tgaacgtctc tttcacagcc tcttttttga
     8701 tgagtcatca tacatttttt cgcttaaata cgagatatcc taatgctaag aacaaaacga
     8761 tgtaagctaa attaccgaat agcatttgat tagtagaaag atgagtcaat tgcttcatga
     8821 tctcttcgtt tccaatttgg ttttgcagat tcaacatact gatcggattc catttgatcc
     8881 actcccattt ttggatgagt gcaataagga taccagatat gattgacgaa gcaaaataaa
     8941 agacgattcc tgctgagatg gatgcgccgg aactattgat aaagcaagca atcatcaaca
     9001 ctaaacttaa aatcagccat aaaccgatat aactacccaa agtataagtg aacaagcttt
     9061 ggatcagtgt ttgaccagta gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca
     9121 gtttcatcaa cacagtgaca ataatagcta aaaggtataa ataaacagaa taaataacta
     9181 atgtgatcca tttgcttact aatatttctc ctcgagaata ctttcgataa aggagatttt
     9241 tcaatgtacc atattgtgct tccatcgaaa tgatcgtgct tgcagctgca atcatgataa
     9301 agacaatcca agatgtggca ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg
     9361 tcgtgtattt tttggctaga agtgctgttc ctatgagcaa aacaactaat agaacagata
     9421 aaacagcagt tgactttttc ttgatcaatt taaataattc ttgttttaca agcaatgtca
     9481 tttttcttct tcccccttta ctgaatccag aatttccaat agagaagatt ccaaatcatt
     9541 cgtcagatgc tggacgtctg tcacatggat attttgtgca gacaatagct ggatcacggt
     9601 tcctaataat tcttcgtttt cttttaaaag cttgatttta tcaggatctt ccaaagtata
     9661 tccagcttct gtcaagatcg ttcgagcagc tagatcattt gttgtagaga taacgatgaa
     9721 atcttgcgct gtttggttca attcagccat tgtacattgt ttgatgatct ttccttgatt
     9781 gatcaggatc agctcatcga ccattttttc aagctcgctg agtaagtgac tggagatcaa
     9841 gaaagaggtg ccctgagtag ccaagtcggt aatgattttt cgcacatctc ttgttgcttg
     9901 tggatccaaa ccgttcatcg gctcatctaa aataacgata gacggatggt tcagtaaggc
     9961 aagagcaatt cccaattttt gtttcattcc caacgagtag gatttcgctt ttttgtgaat
    10021 gtacttttcc atcttgagct gattcacgat agtgtcgata gcagatacat cgttggtttc
    10081 agagaataac ttcagatgat catagcctgt taagaatgga tagatacccg ggtattcgat
    10141 caatgctcct acttgttcca accctttatg actagaggga gaaatctcat tttcaccaat
    10201 acagattgtt ccatctgagt aatggatcag tcctaaaata gctttcatga tcgttgtttt
    10261 tccagcgcca ttaggaccta ctaatccaac gattttgcca ggggataagc taaaactgac
    10321 atcgctgagg gcttgtttgt gaccaaagtt cttggataat tcttggaccg aaagactttt
    10381 caaaaatatt cactcctttt ctatagatag tacaagcata cacctgatga gtggagaagt
    10441 cgctaggtca ttggacttat ttcttatttt ttcataaaaa aattgaatat taaataaaaa
    10501 ggcttatttt ttctgatcag tacttattcc aagtgtctag tggccgctag taatcttgat
    10561 gccaatgata ctgatgagta gcatccccac aaagaaccaa gtggaaagat tcagcttatc
    10621 atgaaacaag accacaccaa ttatgaccga accaactgcg ccaatccctg tccagatagg
    10681 ataagagaga ctcatcggca aacttttgat tgcctttgac aaaaagtaaa aactagcaat
    10741 catgccaatc actgtgtaaa aagaataatt gattttagaa aaaccatcac tccatttcat
    10801 catcgttgac cagaatactt ccaaaattcc tgctatgatt aattccatcc atgccatact
    10861 tacattcctc cttaactata aaaaaataca caaaaagagg aaaatcatcc cttcaaagac
    10921 ctaatggaat gatcatcctc tttatcctac ccggtggcag attgtatatc tttttctgta
    10981 gtatagcaca cctatccaga tatcggcaat ctcatacttt ttagtaaagt gtaccaagca
    11041 ctgacggata tatcgttagc ggaaaagaag atgaatgctt tgcaatttca tagaaagaag
    11101 ttgttattta ttaatcattt aaaaaaaaca
//
LOCUS       DACELF010000276         7482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.105, whole genome shotgun sequence.
ACCESSION   DACELF010000276 DACELF010000000
VERSION     DACELF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.105"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW687_002948"
     CDS             109..192
                     /locus_tag="IW687_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0952143.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW687_002949"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW687_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0952144.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW687_002950"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW687_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW687_002951"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW687_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW687_002952"
     CDS             2348..2593
                     /locus_tag="IW687_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0952145.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW687_002953"
     CDS             2696..3565
                     /locus_tag="IW687_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0952146.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW687_002954"
     CDS             3546..4280
                     /locus_tag="IW687_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0952147.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW687_002955"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW687_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW687_002956"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW687_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0952148.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW687_002957"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW687_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0952149.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IW687_002958"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IW687_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IW687_002959"
     CDS             6927..7469
                     /locus_tag="IW687_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0952150.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACELF010000277         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.130, whole genome shotgun sequence.
ACCESSION   DACELF010000277 DACELF010000000
VERSION     DACELF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.130"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW687_002960"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW687_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW687_002961"
     CDS             2326..2901
                     /locus_tag="IW687_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0952151.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW687_002962"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW687_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0952152.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW687_002963"
     CDS             3788..4942
                     /locus_tag="IW687_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0952153.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACELF010000278         4865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.131, whole genome shotgun sequence.
ACCESSION   DACELF010000278 DACELF010000000
VERSION     DACELF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.131"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            364..1275
                     /locus_tag="IW687_002964"
     CDS             364..1275
                     /locus_tag="IW687_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0952154.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1428..1913
                     /gene="vanZ"
                     /locus_tag="IW687_002965"
     CDS             1428..1913
                     /gene="vanZ"
                     /locus_tag="IW687_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0952155.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2200..2529)
                     /locus_tag="IW687_002966"
     CDS             complement(2200..2529)
                     /locus_tag="IW687_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0952156.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2834..3163
                     /locus_tag="IW687_002967"
     CDS             2834..3163
                     /locus_tag="IW687_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0952157.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3153..3440
                     /locus_tag="IW687_002968"
     CDS             3153..3440
                     /locus_tag="IW687_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0952158.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3719..4282)
                     /locus_tag="IW687_002969"
     CDS             complement(3719..4282)
                     /locus_tag="IW687_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0952159.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1705 a          734 c          837 g         1589 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt gaagatgtaa ggcagagcga
       61 tattgcggtc attatctgcg tgcgctgcgg caagatagcc tgataataag actgatcgca
      121 tagaggggtg gtatttcaca ccgcccattg tcaacaggca gttcagcctc gttaaattca
      181 gcatgggtat cacttatgaa aattcatcta cattggtgat aatagtaaat ccagtagggc
      241 gaaataattg actgtaattt acggggcaaa acggcacaat ctcaaacgag attgtgccgt
      301 ttaaggggaa gattctagaa atatttcata cttccaacta tatagttaag gaggagactg
      361 aaaatgaaga agttgttttt tttattgtta ttgttattct taatatactt aggttatgac
      421 tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta tgatcaaaat
      481 cccaaagaac atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca
      541 gaagaacagg tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa
      601 gaaagtgtga agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac
      661 gggttgcttg atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg
      721 gtcaatgatg ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac
      781 tttgatgagc aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt
      841 tatagtgagc ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa
      901 cgagcccctg aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt
      961 tatccagagg acaaaacaga gttaacagga attcaatatg aaccatggca tattcgctat
     1021 gttggtttac cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg
     1081 gattacctaa aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc
     1141 ttttattatc ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag
     1201 atatcaggaa acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact
     1261 aattcaagga ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt
     1321 actagcactt ttggggtaac gttagctttt taatttaaaa cccacgttaa ctaggacatt
     1381 gctatactaa tgatacaact taaacaaaag aattagagga aattatattg ggaaaaatat
     1441 tatctagagg attgctagct ttatatttag tgacactaat ctggttagtg ttattcaaat
     1501 tacaatacaa tattttatca gtatttaatt atcatcaaag aagtcttaac ttgactccat
     1561 ttactgctac tgggaatttc agagagatga tagataatgt tataatcttt attccatttg
     1621 gcttgctttt gaatgtcaat tttaaagaaa tcggattttt acctaagttt gcttttgtac
     1681 tggttttaag tcttactttt gaaataattc aatttatctt cgctattgga gcgacagaca
     1741 taacagatgt aattacaaat actgttggag gctttcttgg actgaaatta tatggtttaa
     1801 gcaataagca tatgaatcaa aaaaaattag acagagttat tatttttgta ggtatacttt
     1861 tgctcgtatt attgctcgtt taccgtaccc atttaagaat aaattacgtg taagatgtct
     1921 aaatcaagca atctgatctt tcatacacat aaagatattg aatgaattgg attagatgga
     1981 aaacgggatg tggggaaact cgcccgtagg tgtgaagtga ggggaaaacc ggtgataaag
     2041 taaaaagctt acctaacact atagtaacaa agaaagccca attatcaatt ttagtgctga
     2101 ggaattggtc tctttaataa atttccttaa cgttgtaaat ccgcattttc ctgacggtac
     2161 ccccaaacta agatttcata gatagatttt ctgataactt taaaaaacac gaatagcgga
     2221 aaggatgtct tcattcgcta aagagtaata aactactttt ccatcttgac gtgattttgc
     2281 cattccatgt ttttttaaaa aacgtaaatg gtgtgacgtt gtagcgacac tagcagtaag
     2341 tatttctgct aaatcacaga cgcacatttc cttataggtt tctaaagcat agaaaatttt
     2401 aattctagaa aaatcgctga aacatttccc taacacaagt aagtttgaaa aatctttttg
     2461 atccaattta ttttttattc catttacttt ttctttatgt acgacagtca cttggcaaac
     2521 ttttttcatt catctcacct cacattcaaa cgattatttg aatgaagtat acctatttga
     2581 tcactctttt gtcaatctac cttaattcca atacactatt ataagcaatt tcatgtattg
     2641 aagtaaactt acttgcaagt gcgtttttga ttccaagtta aaaacatatt attattctac
     2701 ataatttcct aatcaaataa aactcctttg aaaattgttc taaaggagtt ttattatttt
     2761 gtatttctac taaaaatagc gtacaatgta attactaagt aattacgaaa tgtaaaataa
     2821 tggaggaaat aatatgatta caaaaacaag aaaacaagga aattctatta tgttaacggt
     2881 ccctaaagaa ttcgatgtgc ccaatggtgt tgaagtagaa gctaagcttg tagagaatgg
     2941 tatcctttat gagtttgtag agcctaaaaa agaatttttt gattttagtg aagatatttt
     3001 ggctgatatt ctttcagaag gttatagcaa acaagagatc ttaaaagaat ttagaaatag
     3061 aaaaaatgcg ttaacttctg catttaaatc tattgctgaa gatactgttg caaattctaa
     3121 accgatgaca aaagaggagt tggcagcaga aattggctta tgagattcta ccttctaaac
     3181 acgtaataaa gtatctaaaa aaattaaaag agaaaacatt aaaagaacaa tttttaacga
     3241 taatttatga tgagatagca gttcgtcctc attcgggaga acaaaaaacg ggtgatttat
     3301 caggaatttg ggctattgga ttcaagtatg caggtacgac atatcgcgtt gcatatgaaa
     3361 ttaaagataa tgcagtgata cctatcttac tatgtggaac gcatgaaaat ttttatgaac
     3421 aattaaaaaa gattaggtaa gaacccaatg tttcaacttg agaggataga cgacacttta
     3481 aaagataatt tttaacagct gagagttgta ataccctata attttaaacc actaacctat
     3541 atatttatga aaaatagaaa aaattaaaac gcattttaag cctctttcaa attaaaagct
     3601 tcatccttat gcacaaagag ggcttaaaat gcgttatttt atattgtttt ttagtaataa
     3661 taaaaaaatc actctaattc tcccataaat attatagatt cctaaagatt aaaatagatt
     3721 attccctatt gtcttttaag tagcggtaga gtgttgcctt gctaatacca gtcatttccg
     3781 taatttcttt tacgctataa acctgacttt tatacaattt tactgctcga tcaacatctt
     3841 tctggttcac tcgtggacgg ccacctttac gacctctcgc acgtgcactt ttcaaacctt
     3901 ctttggtacg ttcaacaatt aaatcacgtt cgaactgact aaatgcttgg aagacagtca
     3961 tcattaagcg tccgtgaggc gtgctggtat cgaagttttc tttcaagctc accagcttaa
     4021 catggtgttc ttcaaaatag gtaacaaggt caatcaaatc ctttgtacta cgacccaatc
     4081 gagaaaaact ttctacaatt accgtatctc ccggacgaat tttatctttt aaacgattta
     4141 gttggggacg aaccgctttg gttcccgtca ttttctcagt taggatttca ttacaatttt
     4201 gttcattgag taagtctaat tgacgattta gttcttgtga acgtgtactc actcgggcat
     4261 aaccataaat gtatccactc atgcttttct tcctttcgta ctttaattct ggcttgattg
     4321 tatcataaaa gggagttata gatacatagt taatgcaaca agttatgata caaaatatga
     4381 cctcttttat ttaataaaag aggtcatttc attttgtctc ataatcgatt gtttttgata
     4441 cgagtgtgta tacgaaataa acaaaaaaat cttaagtaaa tttctagata cctaatttaa
     4501 tcctaactag tgtatcctaa agttaataac gagtttatca caatgaaaat tataaaatgc
     4561 tcccatatta gatataacat gcaatatttg aattagaact acaaaattta ttcgttttat
     4621 aaagtaaata atgttgagat actaaggaat agaatattat aaggaattga gctgaatgta
     4681 ttggataaaa aaagaataac tttattagag atggactata aaaatgctcg taattttctt
     4741 ctgaagtctg aaagctattt gaatgctcag ctaccagaat atattagttt tgacgaaact
     4801 ttaaaatctg ctaaaaaagt tttaacttcg ccgtttgtat aattaagcga gacaaataaa
     4861 aaagc
//
LOCUS       DACELF010000279         4446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.138, whole genome shotgun sequence.
ACCESSION   DACELF010000279 DACELF010000000
VERSION     DACELF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.138"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            183..749
                     /gene="amaP"
                     /locus_tag="IW687_002970"
     CDS             183..749
                     /gene="amaP"
                     /locus_tag="IW687_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0952160.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            762..950
                     /locus_tag="IW687_002971"
     CDS             762..950
                     /locus_tag="IW687_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0952161.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            963..1520
                     /locus_tag="IW687_002972"
     CDS             963..1520
                     /locus_tag="IW687_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0952162.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1552..1794
                     /locus_tag="IW687_002973"
     CDS             1552..1794
                     /locus_tag="IW687_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0952163.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2135..3349
                     /locus_tag="IW687_002974"
     CDS             2135..3349
                     /locus_tag="IW687_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0952164.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3380..3598
                     /locus_tag="IW687_002975"
                     /pseudo
     CDS             3380..3598
                     /locus_tag="IW687_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4017..>4446
                     /gene="dfrF"
                     /locus_tag="IW687_002976"
     CDS             4017..>4446
                     /gene="dfrF"
                     /locus_tag="IW687_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0952165.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLG"
BASE COUNT         1480 a          693 c          955 g         1318 t
ORIGIN      
        1 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
       61 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
      121 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
      181 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
      241 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
      301 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
      361 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
      421 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
      481 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
      541 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
      601 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
      661 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
      721 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
      781 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
      841 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
      901 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
      961 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     1021 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     1081 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     1141 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     1201 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     1261 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     1321 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     1381 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     1441 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     1501 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     1561 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     1621 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     1681 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     1741 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     1801 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     1861 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     1921 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     1981 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     2041 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     2101 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     2161 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     2221 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     2281 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     2341 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     2401 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     2461 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     2521 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     2581 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     2641 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     2701 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     2761 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     2821 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     2881 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     2941 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     3001 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     3061 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     3121 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     3181 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     3241 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     3301 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     3361 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     3421 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     3481 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     3541 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt
     3601 ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat
     3661 attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt tgttgccgtg
     3721 aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc tatggtttgt
     3781 caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata ggctgataag
     3841 taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata taatgcagat
     3901 gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag tttgttaaga
     3961 tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg aaatagatga
     4021 taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt aatataccat
     4081 ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat gtggttatta
     4141 tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg aatattgttg
     4201 tttccaccac aacagagtat caaggagata atttagtttc agttaaatca ttagaagatg
     4261 cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga ctatttaagg
     4321 aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat attgaagatg
     4381 gagatacatt ctttccagaa tttgatatca atgattttga agttttgata ggggaaacac
     4441 ttggtg
//
LOCUS       DACELF010000280         3558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.151, whole genome shotgun sequence.
ACCESSION   DACELF010000280 DACELF010000000
VERSION     DACELF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.151"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..399
                     /locus_tag="IW687_002977"
     CDS             <1..399
                     /locus_tag="IW687_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0952166.1"
                     /translation="SPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYL
                     SRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKG
                     YHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..559
                     /locus_tag="IW687_002978"
                     /pseudo
     CDS             509..559
                     /locus_tag="IW687_002978"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            593..1969
                     /gene="tet(L)"
                     /locus_tag="IW687_002979"
     CDS             593..1969
                     /gene="tet(L)"
                     /locus_tag="IW687_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0952167.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2533..>3558
                     /locus_tag="IW687_002980"
     CDS             2533..>3558
                     /locus_tag="IW687_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0952168.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKIS"
BASE COUNT         1172 a          523 c          727 g         1136 t
ORIGIN      
        1 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
       61 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
      121 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
      181 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
      241 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
      301 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
      361 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata
      421 aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt
      481 aaaggaggaa ttaattatga agtgaaatga atgtaacagg gttcaattaa aagagggaag
      541 cgtatcatta accctataaa ctacgtctgc cctcattatt ggagggtgaa atgtgaatac
      601 atcctattca caatcgaatt tacgacacaa ccaaatttta atttggcttt gcattttatc
      661 tttttttagc gtattaaatg aaatggtttt gaacgtctca ttacctgata ttgcaaatga
      721 ttttaataaa ccacctgcga gtacaaactg ggtgaacaca gcctttatgt taaccttttc
      781 cattggaaca gctgtatatg gaaagctatc tgatcaatta ggcatcaaaa ggttactcct
      841 atttggaatt ataataaatt gtttcgggtc ggtaattggg tttgttggcc attctttctt
      901 ttccttactt attatggctc gttttattca aggggctggt gcagctgcat ttccagcact
      961 cgtaatggtt gtagttgcgc gctatattcc aaaggaaaat aggggtaaag catttggtct
     1021 tattggatcg atagtagcca tgggagaagg agtcggtcca gcgattggtg gaatgatagc
     1081 ccattatatt cattggtcct atcttctact cattcctatg ataacaatta tcactgttcc
     1141 gtttcttatg aaattattaa agaaagaagt aaggataaaa ggtcattttg atatcaaagg
     1201 aattatacta atgtctgtag gcattgtatt ttttatgttg tttacaacat catatagcat
     1261 ttcttttctt atcgttagcg tgctgtcatt cctgatattt gtaaaacata tcaggaaagt
     1321 aacagatcct tttgttgatc ccggattagg gaaaaatata ccttttatga ttggagttct
     1381 ttgtggggga attatatttg gaacagtagc agggtttgtc tctatggttc cttatatgat
     1441 gaaagatgtt caccagctaa gtactgccga aatcggaagt gtaattattt tccctggaac
     1501 aatgagtgtc attattttcg gctacattgg tgggatactt gttgatagaa gaggtccttt
     1561 atacgtgtta aacatcggag ttacatttct ttctgttagc tttttaactg cttcctttct
     1621 tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag gtgggctttc
     1681 gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc aggaagctgg
     1741 tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag gtattgcaat
     1801 tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta tggaagttga
     1861 tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca ttgtcattag
     1921 ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa tcgttaaggg
     1981 atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat tcaaatcttt
     2041 ttgttccatt aaagggcgcg attgctgaat aaaagatacg agagacctct cttgtatctt
     2101 ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata aaaattttat
     2161 tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa attggcaagg
     2221 gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt cccttgctga
     2281 tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg gcaggttttt
     2341 ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt tttggtcggc
     2401 actgccgaca gcctcgcaga gcacacactt tatgaatata aagtatagtg tgttatactt
     2461 tacttggaag tggttgccgg aaagagcgaa aatgcctcac atttgtgcca cctaaaaagg
     2521 agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc agctggacta
     2581 aaaggcatgc aatttcataa tcaaagagag cgaaaaagta gaacgaatga tgatattgac
     2641 catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata aaaatattga ttacaacgaa
     2701 cgtgtcaaag aaattattga atcacaaaaa acaggtacaa gaaaaacgag gaaagatgct
     2761 gttcttgtaa atgagttgct agtaacatct gaccgagatt tttttgagca actggatcca
     2821 ggagaacaaa aacgattttt tgaggaaagt tataaattat tttccgaacg atatggcaag
     2881 caaaatattg cttatgcaac agttcataat gatgagcaaa cccctcacat gcatttaggt
     2941 gttgtgccta tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg tcaagaactg
     3001 ttatggctac aagataaatt ccccgagcat atgaaaaaac agggttttga gttgaagcgt
     3061 ggtgaacgtg gctctgaccg taaacatatt gagacagcta aatttaaaaa acaaactttg
     3121 gaaaaagaga ttgattttct agaaaaaaat ttagcagtta aaaaagatga atggactgct
     3181 tatagcgata aagttaaatc agatttagaa gtaccagcga aacgacacat gaaaagtgtt
     3241 gaagtgccaa cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat gaaaacagaa
     3301 aagaaaccaa ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt agtgactgct
     3361 gcgagagata acgataggtt aaaacagcat gttagaaatc tcatgagtac tgatatggcg
     3421 agagaatata aaaaattaag taaagaacat gggcaagtta aagaaaaata tagtggtctt
     3481 gtagagcgat ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga aaacaagtct
     3541 ttaaagtcta aaataagc
//
LOCUS       DACELF010000281         2753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.170, whole genome shotgun sequence.
ACCESSION   DACELF010000281 DACELF010000000
VERSION     DACELF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.170"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..966
                     /gene="vanH"
                     /locus_tag="IW687_002981"
     CDS             7..966
                     /gene="vanH"
                     /locus_tag="IW687_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanH-A/VanH-Pt family D-lactate dehydrogenase"
                     /protein_id="HAQ0952169.1"
                     /translation="MGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSAPF
                     NQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSPD
                     SVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMK
                     QGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLL
                     KLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            959..1990
                     /gene="vanA"
                     /locus_tag="IW687_002982"
     CDS             959..1990
                     /gene="vanA"
                     /locus_tag="IW687_002982"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0952170.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            1996..2604
                     /locus_tag="IW687_002983"
     CDS             1996..2604
                     /locus_tag="IW687_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0952171.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          818 a          530 c          678 g          727 t
ORIGIN      
        1 aataacatcg gcattactgt ttatggatgt gagcaggatg aggcagatgc attccatgct
       61 ctttcgcctc gctttggcgt tatggcaacg ataattaacg ccaacgtgtc ggaatccaac
      121 gccaaatccg cgcctttcaa tcaatgtatc agtgtgggac ataaatcaga gatttccgcc
      181 tctattcttc ttgcgctgaa gagagccggt gtgaaatata tttctacccg aagcatcggc
      241 tgcaatcata tagatacaac tgctgctaag agaatgggca tcactgtcga caatgtggcg
      301 tactcgccgg atagcgttgc cgattatact atgatgctaa ttcttatggc agtacgcaac
      361 gtaaaatcga ttgtgcgctc tgtggaaaaa catgatttca ggttggacag cgaccgtggc
      421 aaggtactca gcgacatgac agttggtgtg gtgggaacgg gccagatagg caaagcggtt
      481 attgagcggc tgcgaggatt tggatgtaaa gtgttggctt atagtcgcag ccgaagtata
      541 gaggtaaact atgtaccgtt tgatgagttg ctgcaaaata gcgatatcgt tacgcttcat
      601 gtgccgctca atacggatac gcactatatt atcagccacg aacaaataca gagaatgaag
      661 caaggagcat ttcttatcaa tactgggcgc ggtccacttg tagataccta tgagttggtt
      721 aaagcattag aaaacgggaa actgggcggt gccgcattgg atgtattgga aggagaggaa
      781 gagtttttct actctgattg cacccaaaaa ccaattgata atcaattttt acttaaactt
      841 caaagaatgc ctaacgtgat aatcacaccg catacggcct attataccga gcaagcgttg
      901 cgtgataccg ttgaaaaaac cattaaaaac tgtttggatt ttgaaaggag acaggagcat
      961 gaatagaata aaagttgcaa tactgtttgg gggttgctca gaggagcatg acgtatcggt
     1021 aaaatctgca atagagatag ccgctaacat taataaagaa aaatacgagc cgttatacat
     1081 tggaattacg aaatctggtg tatggaaaat gtgcgaaaaa ccttgcgcgg aatgggaaaa
     1141 cgacaattgc tattcagctg tactctcgcc ggataaaaaa atgcacggat tacttgttaa
     1201 aaagaaccat gaatatgaaa tcaaccatgt tgatgtagca ttttcagctt tgcatggcaa
     1261 gtcaggtgaa gatggatcca tacaaggtct gtttgaattg tccggtatcc cttttgtagg
     1321 ctgcgatatt caaagctcag caatttgtat ggacaaatcg ttgacataca tcgttgcgaa
     1381 aaatgctggg atagctactc ccgccttttg ggttattaat aaagatgata ggccggtggc
     1441 agctacgttt acctatcctg tttttgttaa gccggcgcgt tcaggctcat ccttcggtgt
     1501 gaaaaaagtc aatagcgcgg acgaattgga ctacgcaatt gaatcggcaa gacaatatga
     1561 cagcaaaatc ttaattgagc aggctgtttc gggctgtgag gtcggttgtg cggtattggg
     1621 aaacagtgcc gcgttagctg ttggcgaggt ggaccaaatc aggctgcagt acggaatctt
     1681 tcgtattcat caggaagtcg agccggaaaa aggctctgaa aacgcagtta taaccgttcc
     1741 cgcagacctt tcagcagagg agcgaggacg gatacaggaa acggcaaaaa aaatatataa
     1801 agcgctcggc tgtagaggtc tagcccgtgt ggatatgttt ttacaagata acggccgcat
     1861 tgtactgaac gaagtcaata ctctgcccgg tttcacgtca tacagtcgtt atccccgtat
     1921 gatggccgct gcaggtattg cacttcccga actgattgac cgcttgatcg tattagcgtt
     1981 aaaggggtga taagcatgga aataggattt acttttttag atgaaatagt acacggtgtt
     2041 cgttgggacg ctaaatatgc cacttgggat aatttcaccg gaaaaccggt tgacggttat
     2101 gaagtaaatc gcattgtagg gacatacgag ttggctgaat cgcttttgaa ggcaaaagaa
     2161 ctggctgcta cccaagggta cggattgctt ctatgggacg gttaccgtcc taatcgtgct
     2221 gtaaactgtt ttatgcaatg ggctgcacag ccggaaaata acctgacaaa ggaaagttat
     2281 tatcccaata ttgaccgaac tgagatgatt tcaaaaggat acgtggcttc aaaatcaagc
     2341 catagccgcg gcagtgccat tgatcttacg ctttatcgat tagacacggg tgagcttgta
     2401 ccaatgggga gccgatttga ttttatggat gaacgctctc atcatgcggc aaatggaata
     2461 tcatgcaatg aagcgcaaaa tcgcagacgt ttgcgctcca tcatggaaaa cagtgggttt
     2521 gaagcatata gcctcgaatg gtggcactat gtattaagag acgaaccata ccccaatagc
     2581 tattttgatt tccccgttaa ataaactttt aaccgttgca cggacaaact atataagcta
     2641 actctttcgg caggaaaccc gacgtatgta actggttctt agggaattta tatatagtag
     2701 atagtattga agaggttctg ttgcaaagtt ttaaataaag aataaaatcc ctt
//
LOCUS       DACELF010000282         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.185, whole genome shotgun sequence.
ACCESSION   DACELF010000282 DACELF010000000
VERSION     DACELF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.185"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002984"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952172.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2051..2101
                     /locus_tag="IW687_002985"
                     /pseudo
     CDS             2051..2101
                     /locus_tag="IW687_002985"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          759 a          336 c          452 g          647 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctat ggaaattata
      481 atcaaacaga aagtagagct gcaccctaat atgtgtgtga tgagctgtac ggaacctgag
      541 caatgggatg tggtaataga aggaaatgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgaaat gaatgtaaca gggttcaatt aaaagaggga agcgtatcat taaccctata
     2101 aactacgtct gccctcatta ttggagggtg aaatgtgaat acatcctatt cacaatcgaa
     2161 tttacgacac aaccaaattt taatttggct ttgc
//
LOCUS       DACELF010000283         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.186, whole genome shotgun sequence.
ACCESSION   DACELF010000283 DACELF010000000
VERSION     DACELF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.186"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002986"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952173.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2051..2101
                     /locus_tag="IW687_002987"
                     /pseudo
     CDS             2051..2101
                     /locus_tag="IW687_002987"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          758 a          339 c          450 g          647 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgaaat gaatgtaaca gggttcaatt aaaagaggga agcgtatcat taaccctata
     2101 aactacgtct gccctcatta ttggagggtg aaatgtgaat acatcctatt cacaatcgaa
     2161 tttacgacac aaccaaattt taatttggct ttgc
//
LOCUS       DACELF010000284         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.187, whole genome shotgun sequence.
ACCESSION   DACELF010000284 DACELF010000000
VERSION     DACELF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.187"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002988"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952174.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2051..2101
                     /locus_tag="IW687_002989"
                     /pseudo
     CDS             2051..2101
                     /locus_tag="IW687_002989"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          758 a          336 c          453 g          647 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctat ggaaattata
      481 atcaaacaga aagtagagct gcaccctaat atgtgtgtga tgagctgtac ggaacctgag
      541 caatgggatg tggtaataga aggaaatgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgaaat gaatgtaaca gggttcaatt aaaagaggga agcgtatcat taaccctata
     2101 aactacgtct gccctcatta ttggagggtg aaatgtgaat acatcctatt cacaatcgaa
     2161 tttacgacac aaccaaattt taatttggct ttgc
//
LOCUS       DACELF010000285         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.188, whole genome shotgun sequence.
ACCESSION   DACELF010000285 DACELF010000000
VERSION     DACELF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.188"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002990"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IW687_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952175.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2051..2101
                     /locus_tag="IW687_002991"
                     /pseudo
     CDS             2051..2101
                     /locus_tag="IW687_002991"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          757 a          339 c          451 g          647 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgaaat gaatgtaaca gggttcaatt aaaagaggga agcgtatcat taaccctata
     2101 aactacgtct gccctcatta ttggagggtg aaatgtgaat acatcctatt cacaatcgaa
     2161 tttacgacac aaccaaattt taatttggct ttgc
//
LOCUS       DACELF010000286         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.189, whole genome shotgun sequence.
ACCESSION   DACELF010000286 DACELF010000000
VERSION     DACELF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.189"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002992"
     CDS             22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952176.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYSP
                     VSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVD
                     TQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPN
                     SRIDKVRYMFNKIT"
     gene            2048..2098
                     /locus_tag="IW687_002993"
                     /pseudo
     CDS             2048..2098
                     /locus_tag="IW687_002993"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          758 a          336 c          452 g          645 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctat ggaaattata
      481 atcaaacaga aagtagagct gcaccctaat atgtgtgtga tgagctgtac ggaacctgag
      541 caatgggatg tggtaataga aggaaatgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttataca gccctgttag taccccagca
     1561 gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa
     1621 ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca
     1681 tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag
     1741 gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact
     1801 ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc
     1861 ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg
     1921 ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata
     1981 agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaat taattatgaa
     2041 gtgaaatgaa tgtaacaggg ttcaattaaa agagggaagc gtatcattaa ccctataaac
     2101 tacgtctgcc ctcattattg gagggtgaaa tgtgaataca tcctattcac aatcgaattt
     2161 acgacacaac caaattttaa tttggctttg c
//
LOCUS       DACELF010000287         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.190, whole genome shotgun sequence.
ACCESSION   DACELF010000287 DACELF010000000
VERSION     DACELF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.190"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002994"
     CDS             22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952177.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYSP
                     VSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVD
                     TQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPN
                     SRIDKVRYMFNKIT"
     gene            2048..2098
                     /locus_tag="IW687_002995"
                     /pseudo
     CDS             2048..2098
                     /locus_tag="IW687_002995"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          757 a          339 c          450 g          645 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttataca gccctgttag taccccagca
     1561 gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa
     1621 ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca
     1681 tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag
     1741 gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact
     1801 ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc
     1861 ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg
     1921 ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata
     1981 agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaat taattatgaa
     2041 gtgaaatgaa tgtaacaggg ttcaattaaa agagggaagc gtatcattaa ccctataaac
     2101 tacgtctgcc ctcattattg gagggtgaaa tgtgaataca tcctattcac aatcgaattt
     2161 acgacacaac caaattttaa tttggctttg c
//
LOCUS       DACELF010000288         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.191, whole genome shotgun sequence.
ACCESSION   DACELF010000288 DACELF010000000
VERSION     DACELF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.191"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002996"
     CDS             22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952178.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYSP
                     VSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVD
                     TQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPN
                     SRIDKVRYMFNKIT"
     gene            2048..2098
                     /locus_tag="IW687_002997"
                     /pseudo
     CDS             2048..2098
                     /locus_tag="IW687_002997"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          757 a          336 c          453 g          645 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctat ggaaattata
      481 atcaaacaga aagtagagct gcaccctaat atgtgtgtga tgagctgtac ggaacctgag
      541 caatgggatg tggtaataga aggaaatgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttataca gccctgttag taccccagca
     1561 gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa
     1621 ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca
     1681 tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag
     1741 gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact
     1801 ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc
     1861 ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg
     1921 ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata
     1981 agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaat taattatgaa
     2041 gtgaaatgaa tgtaacaggg ttcaattaaa agagggaagc gtatcattaa ccctataaac
     2101 tacgtctgcc ctcattattg gagggtgaaa tgtgaataca tcctattcac aatcgaattt
     2161 acgacacaac caaattttaa tttggctttg c
//
LOCUS       DACELF010000289         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.192, whole genome shotgun sequence.
ACCESSION   DACELF010000289 DACELF010000000
VERSION     DACELF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.192"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002998"
     CDS             22..1938
                     /gene="tet(M)"
                     /locus_tag="IW687_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0952179.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYSP
                     VSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVD
                     TQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPN
                     SRIDKVRYMFNKIT"
     gene            2048..2098
                     /locus_tag="IW687_002999"
                     /pseudo
     CDS             2048..2098
                     /locus_tag="IW687_002999"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
BASE COUNT          756 a          339 c          451 g          645 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttataca gccctgttag taccccagca
     1561 gattttcgga tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa
     1621 ttgttagagc catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca
     1681 tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag
     1741 gtcattctta gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact
     1801 ttctttacaa atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc
     1861 ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg
     1921 ttcaataaaa taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata
     1981 agatgaaata ttttttaata aagatttgaa ttaaagtgta aaggaggaat taattatgaa
     2041 gtgaaatgaa tgtaacaggg ttcaattaaa agagggaagc gtatcattaa ccctataaac
     2101 tacgtctgcc ctcattattg gagggtgaaa tgtgaataca tcctattcac aatcgaattt
     2161 acgacacaac caaattttaa tttggctttg c
//
LOCUS       DACELF010000290         1083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.236, whole genome shotgun sequence.
ACCESSION   DACELF010000290 DACELF010000000
VERSION     DACELF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.236"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            470..1018
                     /gene="aac(6')"
                     /locus_tag="IW687_003000"
     CDS             470..1018
                     /gene="aac(6')"
                     /locus_tag="IW687_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0952180.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          394 a          158 c          211 g          320 t
ORIGIN      
        1 tgaacctaag acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata
       61 agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc
      121 ctcttagtat ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat
      181 taagattatt atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc
      241 aaaagataat ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc
      301 tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta taagtaagat
      361 aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg agaacatttc
      421 gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta tgataatcag
      481 tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac tgagactgac
      541 ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga tgaatccaga
      601 acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg gtgcaatccc
      661 tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct cccgacgaaa
      721 gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt ccagaggagg
      781 aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa gtcaaacgga
      841 cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg aacatccgta
      901 tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg caaatggctg
      961 ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt ctcaataata
     1021 aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt acgaaaatta
     1081 ctt
//
LOCUS       DACELF010000291          763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.269, whole genome shotgun sequence.
ACCESSION   DACELF010000291 DACELF010000000
VERSION     DACELF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.269"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..644
                     /locus_tag="IW687_003001"
     CDS             <1..644
                     /locus_tag="IW687_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAQ0952181.1"
                     /translation="DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMH
                     GLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKK
                     CLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDIL
                     RMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          327 a           54 c          131 g          251 t
ORIGIN      
        1 gtgatgaatt atctatacta ggttataaag aaattaaagg aactttttta acaccagaaa
       61 tttattctac tatgtcagaa gaagaacaaa atttgttaaa acgagatatt gccagttttt
      121 taagacaaat gcacggttta gattatacag atattagtga atgtactatt gataataaac
      181 aaaatgtatt agaagagtat atattgttgc gtgaaactat ttataatgat ttaactgata
      241 tagaaaaaga ttatatagaa agttttatgg aaagactaaa tgcaacaaca gtttttgagg
      301 gtaaaaagtg tttatgccat aatgatttta gttgtaatca tctattgtta gatggcaata
      361 atagattaac tggaataatt gattttggag attctggaat tatagatgaa tattgtgatt
      421 ttatatactt acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat
      481 taagaatgta tggaaatata gatattgaga aagcaaaaga atatcaagat atagttgaag
      541 aatattatcc tattgaaact attgtttatg gaattaaaaa tattaaacag gaatttatcg
      601 aaaatggtag aaaagaaatt tataaaagga cttataaaga ttgattatat aatatatgaa
      661 aagctattat aaaagacatt agtattaaat agtttaaaaa aatgaaaaat aataaaggaa
      721 gtgagtcaag tccagactcc tgtgtaaaat gctatacaat gtt
//
LOCUS       DACELF010000292          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E34
            SAMN07141389-rid9869123.guided.271, whole genome shotgun sequence.
ACCESSION   DACELF010000292 DACELF010000000
VERSION     DACELF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141389
            Sequence Read Archive: SRR5811509
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740839.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740839.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2020 22:27:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,956
            Genes (coding)                    :: 2,880
            CDSs (with protein)               :: 2,880
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 29 of 76
            Pseudo Genes (incomplete)         :: 43 of 76
            Pseudo Genes (internal stop)      :: 14 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141389-rid9869123.guided.271"
                     /isolate="E34"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>742
                     /locus_tag="IW687_003002"
     CDS             <1..>742
                     /locus_tag="IW687_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0952182.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIK"
BASE COUNT          312 a           65 c          121 g          244 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aa
//
